Analytical Methods最新文献

{"title":"First-in-class hydrazone–pyrazoline sensors for selective detection of Zn2+, Cd2+, and Hg2+ in aqueous environments†","authors":"Alexander Ciupa","doi":"10.1039/D5AY00639B","DOIUrl":"10.1039/D5AY00639B","url":null,"abstract":"<p >Six first-in-class hydrazone–pyrazoline fluorescent sensors that selectively detect Zn<small>²⁺</small>, Cd<small>²⁺</small>, and Hg<small>²⁺</small> at three distinct wavelengths in aqueous environments are reported. These novel hybrid sensors are synthesized in just two steps from inexpensive, commercially available materials, enabling rapid generation and screening for desirable photophysical properties. Lead sensor <strong>P1</strong> was validated for <em>in situ</em> detection of group 12 metals in river and pond water using a portable, low-cost device. Crucially, this system enabled rapid, naked-eye detection of toxic metals directly in the environment without the need for expensive instrumentation or mains electricity. These findings establish a new direction in pyrazoline sensor research and set a high benchmark for future sensor development.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 27","pages":" 5782-5787"},"PeriodicalIF":2.7,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ay/d5ay00639b?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in upconversion nanoparticles for biomolecule detection: a review","authors":"Shuoshuo Cheng, Bin Huang, Kangqiang Lu, Jinhui Li, Herui Wen and Jialin Zhang","doi":"10.1039/D5AY00631G","DOIUrl":"10.1039/D5AY00631G","url":null,"abstract":"<p >Given the continuously increasing requirements for biological detection, upconversion nanoparticle (UCNP)-based techniques offer a novel research direction for biomolecule detection. Due to their superior and stable photophysical characteristics, UCNPs have received widespread attention and have been extensively applied for reliable and highly sensitive detection of biomolecules. Herein, we review articles based on three aspects: UCNP synthesis, surface functionalization, and application in biomolecule detection. The future development trend of UCNPs is also anticipated. Several synthesis methods are introduced, and the advantages and disadvantages of these synthesis methods are summarized and compared. Control of UCNP size and shape, as well as UCNP doping, is discussed. For surface modification, we introduce approaches such as surface silanization, ligand exchange, ligand oxidation, ligand attraction and electrostatic layer by layer assembly. In addition, we focus on UCNP-based luminescence sensors for the analysis of biomolecules, such as nucleic acids, amino acids, proteins, carbohydrates, uric acid and lipids. These upconversion nanomaterials were used as carriers for recognition, transmission or signal amplification and significantly improved the performance of biomolecule detection. Finally, the shortcomings of UCNP are analyzed and its future prospects are presented. This review provides new insights into the application of upconversion nanoparticles as an important element in biomolecule detection.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 27","pages":" 5563-5578"},"PeriodicalIF":2.7,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concentration dependence of Kubelka–Munk function and apparent absorbance button sample holder infrared spectra","authors":"Robert L. White","doi":"10.1039/D5AY00758E","DOIUrl":"10.1039/D5AY00758E","url":null,"abstract":"<p >Button sample holder infrared spectra in Kubelka–Munk function and apparent absorbance formats are evaluated. Trends in plots of vibration band area and peak intensity <em>versus</em> concentration are compared. Two test sample powders were comprised of different concentrations of caffeine dispersed in potassium chloride and copper thiosulfate mixed with kaolinite and potassium chloride in different ratios. When infrared spectra of caffeine and copper thiosulfate were measured by diffuse reflection, Kubelka–Munk calibration functions tended to be linear whereas apparent absorbance functions were curved. However, over narrow concentration ranges, both formats provided linear plots and exhibited similar sensitivities. For samples with the highest clay content, the kaolinite O–H stretching and inorganic oxide vibration band area <em>versus</em> concentration plots were linear over wider ranges than the Kubelka–Munk function plots.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 26","pages":" 5519-5526"},"PeriodicalIF":2.7,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ay/d5ay00758e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A highly sensitive flow injection chemiluminescence method for myoglobin detection in beef samples based on zinc ion-induced “dragging” of heme iron†","authors":"Yali Zhang, Yuhan Ma, Yapin Zhao and Jing Ha","doi":"10.1039/D5AY00774G","DOIUrl":"10.1039/D5AY00774G","url":null,"abstract":"<p >We report for the first time that zinc ions (Zn<small>²⁺</small>) significantly enhance the chemiluminescence (CL) signal of the luminol–hydrogen peroxide (luminol–H<small>₂</small>O<small>₂</small>) system by “dragging” heme iron out of myoglobin (Mb). Integrating this with myoglobin aptamer (Mb aptamer) specific recognition enables sensitive and specific quantification of Mb content in beef samples.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 25","pages":" 5155-5159"},"PeriodicalIF":2.7,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/Cas12a-assisted electrochemiluminescent detection of ochratoxin A based on COF@Ru coupled with a DNA tetrahedral scaffold †","authors":"Yang Cao, Chun Huang, Kui Li, Xi Zhang, Mengfei Tong, Jiale Tu, Kun Yang, Shuai Yuan and Hong Zhang","doi":"10.1039/D5AY00427F","DOIUrl":"10.1039/D5AY00427F","url":null,"abstract":"<p >To sensitively detect ochratoxin A (OTA), a CRISPR/Cas12a-assisted electrochemiluminescence (ECL) aptasensor based on COF@Ru coupled with a DNA tetrahedral scaffold (DTS) was successfully fabricated. The covalent organic framework (COF) acted as a confined micro-reactor for the tris(2,2′-bipyridyl)ruthenium(<small>II</small>)/tri-<em>n</em>-propylamine (Ru(bpy)<small>₃</small><small>²⁺</small>/TPrA) system and hence, evidently enhanced ECL signals. Au nanoparticles (AuNPs) coupled with DTS caused more ferrocene (Fc)-modified quenching DNA probe molecules to be attached to the surface of the sensing electrode, which could reduce the detection background. Activated CRISPR/Cas12a could effectively amplify the ECL signal since it could cut off a considerable amount of quenching DNA probe molecules and make them move away from the electrode. Owing to these strategies, the fabricated ECL aptasensor could detect OTA as low as 3.5 fg mL<small>⁻¹</small> in a linear detection range of 10<small>⁻⁵</small>–100 ng mL<small>⁻¹</small>. Furthermore, the CRISPR/Cas12a and COF@Ru coupled with DTS-based ECL biosensor possessed high stability and specificity. More importantly, the ECL biosensor could effectively detect OTA in food samples, indicating that it can play a role in monitoring OTA levels in the field of food safety.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 25","pages":" 5298-5307"},"PeriodicalIF":2.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous and sensitive determination of hydroquinone, catechol, and resorcinol with a palladium nanoparticles/poly(bromocresol green) modified glassy carbon electrode†","authors":"Çağrı Ceylan Koçak","doi":"10.1039/D5AY00511F","DOIUrl":"10.1039/D5AY00511F","url":null,"abstract":"<p >In this study, catechol (CC), hydroquinone (HQ), and resorcinol (RC) – dihydroxybenzene isomers – were determined simultaneously using the composite surface. The proposed material was prepared <em>via</em> electrochemical polymerization of Bromocresol Green (BCG) followed by electrodeposition of palladium nanoparticles (Pd). The developed electrode was characterized using X-ray Photoelectron Spectroscopy (XPS), Scanning Electron Microscopy (SEM), Electrochemical Impedance Spectroscopy (EIS), and X-ray Energy Dispersive Spectroscopy (EDX) techniques. Electrochemical measurements were performed using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) in pH 7 phosphate buffer solution. The Pd nanoparticle deposited polymerized BCG-modified glassy carbon electrode (Pd/Poly(BCG)/GCE) displayed enhanced current sensitivity for all three isomers compared to a bare GCE and Poly(BCG)/GCE. Thus, this was attributed to the synergistic effect of the improved active surface area of Poly(BCG) and the unique properties of Pd nanoparticles. Under optimized conditions, three well-separated oxidation peaks were achieved by DPV for HQ (19 mV), CC (130 mV), and RC (430 mV) at the Pd/Poly(BCG)/GCE during the simultaneous concentration change of the isomer mixture. The limit of detection values (S/N = 3) were found to be 89 nM, 74 nM, and 98 nM for HQ, CC, and RC, respectively. Thus, the high sensitivity, adequate reproducibility, good stability, and easy preparation of the Pd/Poly(BCG)/GCE surface made it a promising candidate for the determination of dihydroxybenzene isomers.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 27","pages":" 5662-5671"},"PeriodicalIF":2.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aptamer-based biosensors for tobramycin†","authors":"Jizhao Zhang and Qiang Zhao","doi":"10.1039/D5AY00680E","DOIUrl":"10.1039/D5AY00680E","url":null,"abstract":"<p >The widespread application of tobramycin has caused significant environmental issues and health risks in recent years. Currently, the simple, rapid, sensitive and selective detection of tobramycin is in high demand. Since the emergence of aptamers against tobramycin, aptamer-based biosensors have exhibited great potential for tobramycin detection. In this review, we comprehensively introduce the selection and study of DNA and RNA aptamers for tobramycin and summarize recent advances in biosensors, such as electrochemical sensors, colorimetric sensors, and fluorescence sensors, for tobramycin using aptamers as recognition elements. We elucidate the fundamental principles of these aptamer-based biosensors and their applications in tobramycin detection and the advantages and pitfalls of different types of aptamer-based biosensors. Aptamer-based biosensors show promise in environmental monitoring, medical health and other domains.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 27","pages":" 5605-5620"},"PeriodicalIF":2.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the link between polymeric surface composition and emerging bisphenol release in toys and childcare products: insights from the Swiss market†","authors":"Camille Rime, Sanja Delic, Anne Onidi, Lionel Cretegny, Davide Staedler and Fiorella Lucarini","doi":"10.1039/D5AY00678C","DOIUrl":"10.1039/D5AY00678C","url":null,"abstract":"<p >This study investigates the release of 14 bisphenols (BPs) from various toys and childcare plastic products available in the Swiss market, using artificial saliva as a simulant. A total of 162 samples were analyzed, revealing substantial differences in BP release across polymer types. Polyethylene (PE) and polypropylene (PP) exhibited the highest release rates, while polyethylene terephthalate (PET) and polyurethane (PUR) showed lower BP release. Statistically significant differences among polymers emphasize the impact of polymer composition on leaching potential. These results enhance the understanding of BP exposure risks from polymeric materials in children's products, underscoring the need for targeted regulatory standards. By identifying high-risk polymers, this study provides valuable guidance for selecting safer materials to reduce BP exposure in products for children.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 25","pages":" 5276-5285"},"PeriodicalIF":2.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ay/d5ay00678c?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel MIP electrochemical sensor based on a CuFe2O4NPs@rGO nanocomposite and its application in breast milk samples for the determination of fipronil†","authors":"Muath Njjar, Ezgi Zekiye Aktürk, Ahmet Kaya, Canan Onac and Abdullah Akdogan","doi":"10.1039/D5AY00911A","DOIUrl":"10.1039/D5AY00911A","url":null,"abstract":"<p > <em>Background</em>: fipronil, a widely utilized insecticide in agriculture, has been shown to have potential health implications as it can accumulate in the environment and affect human health. Electrochemical sensors, specifically those incorporating molecularly imprinted polymers (MIPs), provide an efficient way for the detection of fipronil because of their selectivity and specificity. The combination of CuFe<small>₂</small>O<small>₄</small>NPs and reduced graphene oxide (rGO) exhibits a synergistic effect that enhances sensitivity and selectivity. The composite's effective properties provide a robust platform for fipronil determination in various matrices. This study detected fipronil using an electrochemical sensor based on a glassy carbon electrode (GCE) modified with MIP@CuFe<small>₂</small>O<small>₄</small>NPs@rGO. <em>Results</em>: the synthesized MIP@CuFe<small>₂</small>O<small>₄</small>NPs@rGO material was characterized using various techniques such as Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (FESEM), X-ray diffraction (XRD) analysis, energy dispersive X-ray (EDX) analysis, Brunauer–Emmett–Teller (BET) analysis, X-ray photoelectron spectroscopy (XPS) analysis, and electrochemical impedance spectroscopy (EIS). The modified GCE showed enhanced electrochemical behavior for fipronil, as demonstrated by cyclic voltammetry (CV) and square wave voltammetry (SWV) techniques. Optimization of parameters such as pH, pyrrole concentration, and template concentration further improved sensor performance. The sensor exhibited a linear dynamic range of 1 to 6 nM, with a limit of detection (LOD) of 0.30 nM (S/N = 3) and a limit of quantification (LOQ) of 1.08 nM (S/N = 10), highlighting its sensitivity and reliability. The precision of the method was excellent, with a relative standard deviation of less than 4.0%. When applied to quantify fipronil in breast milk samples, the sensor showed high accuracy and precision, with recoveries ranging from 96.24% to 97.75%. <em>Significance</em>: the sensor offers several advantages, including high sensitivity, specificity, and accuracy. Its ability to detect fipronil in complex matrices such as breast milk highlights its potential for real-world applications in environmental and health monitoring. Overall, this research paves the way for the development of efficient, rapid and eco-friendly sensors for detecting pesticide residues in various environmental and biological samples.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 26","pages":" 5508-5518"},"PeriodicalIF":2.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144482557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BIScreener: enhancing breast cancer ultrasound diagnosis through integrated deep learning with interpretability†","authors":"Yao Chen, Peiling Wang, Jing Ouyang, Miduo Tan, Libo Nie, Yibo Zhang and Tong Wang","doi":"10.1039/D5AY00475F","DOIUrl":"10.1039/D5AY00475F","url":null,"abstract":"<p >Breast cancer is the leading cause of death among women worldwide, and early detection through the standardized BI-RADS framework helps physicians assess the risk of malignancy and guide appropriate diagnostic and treatment decisions. In this study, an interpretable deep learning model (BIScreener) was proposed for predicting BI-RADS classifications from breast ultrasound images, aiding in the accurate assessment of breast cancer risk and improving diagnostic efficiency. BIScreener utilizes the stacked generalization of three pretrained convolutional neural networks to analyze ultrasound images obtained from two specific instruments (Mindray R5 and HITACHI) used at local hospitals. BIScreener achieved a classification total accuracy of 90.0% and ROC-AUC value of 0.982 in the external test set for five BI-RADS categories. The proposed method achieved 83.8% classification total accuracy and 0.967 ROC-AUC value for seven BI-RADS categories. In addition, the model improved the diagnostic accuracy of two radiologists by more than 8.1% for five BI-RADS categories and by more than 4.8% for seven BI-RADS categories and reduced the explanation time by more than 19.0%, demonstrating its potential to accelerate and improve the breast cancer diagnosis process.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 27","pages":" 5704-5713"},"PeriodicalIF":2.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}