Analytical Methods最新文献_第9页

Defining selective chelation windows for Ca²⁺-Mg²⁺ discrimination in saline matrices: comparative performance of EDTA and EGTA. 在盐水基质中定义Ca2+-Mg2+的选择性螯合窗口：EDTA和EGTA的比较性能。

IF 3 3区化学

Analytical Methods Pub Date : 2026-10-20 DOI: 10.1039/d6ay02005d

Ghadeer H Albalawi, Ibtihaj Albalawi, Muhammad Ayaz, Fatimah A Alotaibi, Munawar Iqbal, Atheer G Almashhori, Fahad Ayesh Alharthi, Christopher Michael

{"title":"Defining selective chelation windows for Ca2+-Mg2+ discrimination in saline matrices: comparative performance of EDTA and EGTA.","authors":"Ghadeer H Albalawi, Ibtihaj Albalawi, Muhammad Ayaz, Fatimah A Alotaibi, Munawar Iqbal, Atheer G Almashhori, Fahad Ayesh Alharthi, Christopher Michael","doi":"10.1039/d6ay02005d","DOIUrl":"https://doi.org/10.1039/d6ay02005d","url":null,"abstract":"Selective discrimination of Ca2+ from Mg2+ remains challenging in saline matrices because both ions exhibit similar coordination behaviour and their chelation responses can be altered by solution conditions. EDTA and EGTA were systematically compared for Ca2+ and Mg2+ complexometric titration over 0-1000 ppm. The effects of pH (3-12), temperature (25-45 °C), NaCl (0-30 mM), KCl (0-19 000 mg L-1), SO42- (0-2700 mg L-1), and K+ (0-600 mg L-1) were evaluated in individual and equimolar Ca2+ : Mg2+ systems. Both chelators produced highly linear calibrations, but their responses differed markedly. EGTA increased Ca2+ calibration sensitivity by 32.9% relative to EDTA and showed substantially greater thermal stability. EDTA responded to both metals across pH 3-12, whereas EGTA produced no measurable Mg2+ endpoint at pH 3-7. Crucially, this Ca2+-selective response persisted in mixed Ca2+ : Mg2+ solutions containing chloride, sulphate, and potassium at the investigated concentrations. The study identifies an experimentally observed operational selectivity interval at pH 3-7, defined by differential endpoint detectability, in which Ca2+ remains measurable with EGTA while no stable visual Mg2+ endpoint is obtained under the investigated conditions. This matrix-resilient behaviour provides a practical basis for selective calcium analysis and future Ca2+/Mg2+ separation strategies.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149226756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Label-free fluorescent detection of miRNA-21 via EXPAR-triggered rolling circle amplification coupled with a dimeric/tandem G-quadruplex-thioflavin T reporter. 通过expar触发滚动环扩增和二聚体/串联g -四重-硫黄素T报告基因对miRNA-21进行无标记荧光检测。

IF 3 3区化学

Analytical Methods Pub Date : 2026-10-20 DOI: 10.1039/d6ay01589a

Hanbo Zhang, Yuehua Yuan, Yulin Ren, Yubo Zhao, Yinxiao Qi, Feixue Feng

{"title":"Label-free fluorescent detection of miRNA-21 via EXPAR-triggered rolling circle amplification coupled with a dimeric/tandem G-quadruplex-thioflavin T reporter.","authors":"Hanbo Zhang, Yuehua Yuan, Yulin Ren, Yubo Zhao, Yinxiao Qi, Feixue Feng","doi":"10.1039/d6ay01589a","DOIUrl":"https://doi.org/10.1039/d6ay01589a","url":null,"abstract":"MicroRNA-21 is a well recognized oncogenic microRNA and is involved in the initiation and development of many cancers, making it an important candidate marker for diagnosis. In this work, a label-free fluorescent assay was designed for highly sensitive miRNA-21 detection by coupling exponential amplification reaction (EXPAR), rolling circle amplification (RCA), and dimeric G-quadruplex/thioflavin T (ThT) signal reporting. The designed asymmetric hairpin probe specifically recognizes the target and initiates the EXPAR, generating abundant trigger strands that subsequently activate RCA. The RCA products contain repeated G-rich segments encoding tandem/dimeric G4-forming motifs, which form multiple G-quadruplex units in the presence of K+. After ThT binds to these structures, a clear fluorescence output is generated. Benefiting from the effectively suppressed background leakage by the hairpin design and the significantly enhanced signal generation enabled by the EXPAR-RCA cascade, the assay exhibits excellent analytical performance. The fluorescence response shows a good linear correlation over the range of 10 fM to 10 nM, with a limit of detection (LOD) of 1.58 fM. The method also demonstrates high selectivity toward miRNA-21, capable of discriminating single-base mismatched sequences. Furthermore, analysis of diluted serum and cellular RNA samples demonstrated the preliminary applicability of the proposed strategy in biological matrices. The method provides a convenient, low-cost, and highly sensitive analytical platform for miRNA detection and bioanalytical research.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149226724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An anthocyanin-curcumin colorimetric array with internal standard-guided feature switching for monitoring volatile alkaline nitrogen gases in meat freshness assessment. 一种内部标准导向特征切换的花青素-姜黄素比色阵列，用于肉类新鲜度评估中挥发性碱性氮的监测。

IF 3 3区化学

Analytical Methods Pub Date : 2026-10-20 DOI: 10.1039/d6ay01887d

Ziqin Liao, Yu Bai, Yulin Chen, Jin Shang, Wei Gan, Yuzhu Wang, Xuan Wei

{"title":"An anthocyanin-curcumin colorimetric array with internal standard-guided feature switching for monitoring volatile alkaline nitrogen gases in meat freshness assessment.","authors":"Ziqin Liao, Yu Bai, Yulin Chen, Jin Shang, Wei Gan, Yuzhu Wang, Xuan Wei","doi":"10.1039/d6ay01887d","DOIUrl":"https://doi.org/10.1039/d6ay01887d","url":null,"abstract":"Smartphone-based colorimetric sensor arrays using natural dyes are promising for on-site meat freshness assessment, but varying illumination and imaging angles compromise reliability. To address this, we developed an anthocyanin-curcumin array incorporating an α-Fe2O3 internal standard and an internal-standard-guided dynamic feature-space switching (IS-DFSS) strategy and validated its effectiveness under complex imaging conditions using ammonia as the representative analyte because it is the dominant component of total volatile basic nitrogen (TVB-N). Eight colorimetric films and the internal standard film were hydrophobically modified and assembled into a 3 × 3 array. The water contact angle increased from 63.3° to 112.9°, ensuring signal stability in high humidity. The sensing units responded strongly to TVB-N components ammonia, trimethylamine (TMA), and dimethylamine (DMA). For ammonia, Euclidean distance increased from 1.23 to 24.7 at 50 ppm and 13.7 to 65.7 at 500 ppm over 120 min, while non-alkaline volatiles (ethanol, hexanal) and non-volatile food matrix components (protein, glucose, fat) were negligible. In pork at 4 °C, array response correlated with TVB-N content (R2 = 0.987). The RGB values from 174 ammonia-responsive and 126 environment-interference images were averaged over three replicates to construct datasets, deriving HSV and Lab features. IS-DFSS with CatBoost reduced the mean absolute error to 15.85 ppm (45.46% reduction) and the mean false-signal error to 27.49 ppm (33.97% reduction) versus single RGB features, achieving R2 = 0.972. This method provides a robust, portable platform for monitoring TVB-N components, laying the foundation for reliable meat freshness assessment under complex imaging conditions.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149222891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevention of IED threats: a non-destructive and multiparametric detection of pre-blast explosive residues in fabrics using confocal Raman microscopy. 预防简易爆炸装置威胁：使用共聚焦拉曼显微镜对织物中爆炸前残留物的非破坏性和多参数检测。

IF 3 3区化学

Analytical Methods Pub Date : 2026-10-20 DOI: 10.1039/d5ay00685f

Giuseppina Gullifa, Flavia Iezzi, Antonietta Lombardozzi, Maria Cristina Pigro, Daniela Panetta, Alessandro Ruscitti, Chiara Albertini, Stefano Materazzi, Roberta Risoluti

{"title":"Prevention of IED threats: a non-destructive and multiparametric detection of pre-blast explosive residues in fabrics using confocal Raman microscopy.","authors":"Giuseppina Gullifa, Flavia Iezzi, Antonietta Lombardozzi, Maria Cristina Pigro, Daniela Panetta, Alessandro Ruscitti, Chiara Albertini, Stefano Materazzi, Roberta Risoluti","doi":"10.1039/d5ay00685f","DOIUrl":"https://doi.org/10.1039/d5ay00685f","url":null,"abstract":"Improvised explosive devices (IEDs) are widespread among criminal and terrorist cells since such organizations are more and more capable of creating evermore powerful and easily concealable explosive devices. This makes their detection not only challenging but also crucial to prevent bomb attacks. In particular, textiles are often a source of evidence of contamination as a consequence of manipulating explosives. This work proposes a novel, rapid and accurate confocal Raman microscopy method for the detection and identification of pre-blast explosive residues related to the handling, packaging, and carriage of explosive devices. Raman spectra were collected from explosive residues of TNT, TATP, PETN, Pyrodex, RDX, and ANFO trapped in different types of fibers (natural/synthetic, dyed/undyed) in order to evaluate the spectroscopic response in conditions that are as realistic as possible and to quantify the interfering effect of the substrate. The results demonstrated that all the investigated explosives were correctly identified with 100% accuracy, showing no sample thermodegradation or interfering contributions related to the textile substrate. This innovative approach permits the simultaneous detection of the most common explosives involved in IEDs by a fast, accurate and non-destructive platform able to be used as confirmatory analysis and to provide timely support for forensic investigation for homeland security.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149226690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-channel digital surface-enhanced Raman spectroscopy resolves GPC1⁺MUC4⁺ single-exosome heterogeneity released by PANC-1 and BxPC-3 pancreatic cancer cells. 双通道数字表面增强拉曼光谱分析胰腺癌细胞PANC-1和BxPC-3释放的GPC1+MUC4+单外泌体异质性。

IF 3 3区化学

Analytical Methods Pub Date : 2026-10-20 DOI: 10.1039/d6ay01831a

Xin Wang, Zuoxi Zhang, Bin Zheng

{"title":"Dual-channel digital surface-enhanced Raman spectroscopy resolves GPC1+MUC4+ single-exosome heterogeneity released by PANC-1 and BxPC-3 pancreatic cancer cells.","authors":"Xin Wang, Zuoxi Zhang, Bin Zheng","doi":"10.1039/d6ay01831a","DOIUrl":"https://doi.org/10.1039/d6ay01831a","url":null,"abstract":"Extracellular-vesicle populations average molecular signals across particles and can conceal rare, co-expressing phenotypes. We developed a dual-channel digital surface-enhanced Raman spectroscopy platform that partitions individual CD63-captured vesicles and identifies glypican-1 (GPC1) and mucin 4 (MUC4) with 4-mercaptobenzoic acid and 5,5'-dithiobis(2-nitrobenzoic acid) nanotags. The method was evaluated with eight independent batches from each of PANC-1, BxPC-3, HPDE and hTERT-HPNE cell models, seven blinded PANC-1/BxPC-3 mixtures, artificial-serum spikes and defined interferents. Across 49 256 single-vesicle events, beta-binomial modelling estimated GPC1+MUC4+ fractions of 13.8% for PANC-1 and 37.8% for BxPC-3. The adjusted between-model difference was 24.1 percentage points (batch-cluster bootstrap 95% confidence interval, 21.6-26.5; P = 5.47 × 10-9). The response was linear against log10 concentration from 1.0 × 105 to 1.0 × 109 particles per mL; the extrapolated detection-limit estimate was 1.0 × 104 particles per mL, the overall precision was 6.49% coefficient of variation, and the mean artificial-serum recovery was 100.5%. Blinded mixture estimates tracked the nominal BxPC-3 fraction, while grouped nested cross-validation classified the four cell sources with 90.6% accuracy. Digital SERS agreed with nanoflow cytometry, giving a concordance correlation coefficient of 0.980 and a mean relative bias of 1.51%. These results show that marker co-occurrence, rather than bulk abundance alone, can distinguish pancreatic-cell vesicle phenotypes and provide a quantitative route for studying source-dependent vesicle heterogeneity.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149226684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and application of a prediction system for benzene series emission concentration and inhalation exposure risk of children's toys. 儿童玩具苯系物排放浓度及吸入暴露风险预测系统的开发与应用。

IF 3 3区化学

Analytical Methods Pub Date : 2026-10-19 DOI: 10.1039/d6ay01329e

Lihua Yu, Minghao Wang, Jingmin Wang, Jiannan Hu, Fengyun Han, Didi Wang, Fangzhou Liu, Cuiling Gao, Xiangke He

{"title":"Development and application of a prediction system for benzene series emission concentration and inhalation exposure risk of children's toys.","authors":"Lihua Yu, Minghao Wang, Jingmin Wang, Jiannan Hu, Fengyun Han, Didi Wang, Fangzhou Liu, Cuiling Gao, Xiangke He","doi":"10.1039/d6ay01329e","DOIUrl":"https://doi.org/10.1039/d6ay01329e","url":null,"abstract":"Children's toys possess both entertainment value and the core value of promoting children's physical and mental development. However, although children are exposed to volatile organic compounds in a variety of dyes and auxiliaries present on children's toys, the health hazard and the indoor air quality during playtime are poorly known. Specifically, reports on the exposure concentration and potential risk of volatile organic compounds released by children's toys in different timeframes and environments remain scarce. Here, we construct a prediction system for benzene series emission concentration from children's toys by integrating exposure routes, diffusion coefficients and toxicological data using the finite element method. The emission models of the benzene series from different types of children's toys are derived based on Fick's law and Green's function in this paper. The initial release concentration and diffusion coefficient of organic substances in children's toys are obtained through experiments in an environmental chamber and a thermal desorption gas chromatograph-mass spectrometer. Furthermore, an assessment was conducted on the inhalation exposure of different concentrations of toluene and xylene. For the first time, this system converts abstract chemical risks into immersive visual effects through presentation forms such as color and animation. This system achieved dynamic prediction of the spatial and temporal distribution concentrations of different compounds in various scenarios, and it can guide consumers in the use of children's toys and promote technological innovation in the production of children's toys.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149219629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An acetylcholinesterase electrochemical biosensor based on a three-dimensional nanoporous PdCe nanocubes/graphene interface for malathion determination. 基于三维纳米多孔PdCe纳米立方/石墨烯界面测定马拉硫磷的乙酰胆碱酯酶电化学生物传感器。

IF 3 3区化学

Analytical Methods Pub Date : 2026-10-19 DOI: 10.1039/d6ay01829g

Chunmei Gao, Chengzong Zhu, Hongyu Dai, Qiuyu Tang, Zhida Han, Yuxin Shi, Genhua Zhang, Rongjing Cui

{"title":"An acetylcholinesterase electrochemical biosensor based on a three-dimensional nanoporous PdCe nanocubes/graphene interface for malathion determination.","authors":"Chunmei Gao, Chengzong Zhu, Hongyu Dai, Qiuyu Tang, Zhida Han, Yuxin Shi, Genhua Zhang, Rongjing Cui","doi":"10.1039/d6ay01829g","DOIUrl":"https://doi.org/10.1039/d6ay01829g","url":null,"abstract":"Three-dimensional nanoporous PdCe (NP-PdCe) nanocubes were fabricated by dealloying PdCeAl alloy ribbons and integrated with graphene and chitosan to construct an acetylcholinesterase (AChE)-based electrochemical biosensor for malathion determination. The NP-PdCe nanocubes possess a bicontinuous nanoporous framework, mesoporous channels, and exposed Pd-Ce interfacial sites that provide an accessible surface for enzyme loading and promote thiocholine oxidation. Graphene (GR) forms a conductive network for electron transport, whereas chitosan provides a biocompatible film-forming matrix for enzyme immobilization. Under optimized conditions, the AChE/NP-PdCe/GR-CS/GCE biosensor exhibited two linear ranges (2.0-60 and 60-350 ng mL-1) and a reported detection limit of 1.30 ng mL-1. The sensor showed acceptable repeatability (RSD 3.35%), fabrication reproducibility (RSD 1.72%), four-week response retention (89.45%), and resistance to selected non-pesticide interferents. In practice, the biosensor was employed to detect malathion in tap water and cabbage samples with satisfactory recovery.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149219664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The electrochemiluminescence immunosensor based on the Ce(III,IV)-MOF dual-function platform is used for the ultra-sensitive detection of AFP. 基于Ce(III，IV)-MOF双功能平台的电化学发光免疫传感器用于AFP的超灵敏检测。

IF 3 3区化学

Analytical Methods Pub Date : 2026-10-19 DOI: 10.1039/d6ay01621a

Li Liu, Haoyi Ren, Tiantian Ji, Shushu Li, Hongyu Xue, Chenglin Hong, Hongling Li

{"title":"The electrochemiluminescence immunosensor based on the Ce(III,IV)-MOF dual-function platform is used for the ultra-sensitive detection of AFP.","authors":"Li Liu, Haoyi Ren, Tiantian Ji, Shushu Li, Hongyu Xue, Chenglin Hong, Hongling Li","doi":"10.1039/d6ay01621a","DOIUrl":"https://doi.org/10.1039/d6ay01621a","url":null,"abstract":"To address the challenges of unstable immobilization of tris(2,2'-bipyridine) ruthenium(II) (Ru(bpy)32+) and inefficient generation of the tri-n-propylamine radical (TPrA˙) in the Ru(bpy)32+/TPrA electrochemiluminescence (ECL) system, a novel dual-function strategy using a mixed-valence cerium metal-organic framework (Ce(III,IV)-MOF) was introduced in this study. The Ce(III,IV)-MOF was employed both as a scaffold for Ru(bpy)32+ and as a co-reaction catalyst. The three-dimensional structure of the Ce(III,IV)-MOF provides a stable environment for Ru(bpy)32+, while the Ce(III)/Ce(IV) redox pair within the framework facilitates TPrA oxidation through reversible single-electron transfer, thereby enhancing TPrA˙ generation. This design co-localizes the luminophore and catalytic centers within a single framework, reducing shortening the electron-transfer distance between Ru(bpy)32+ and the TPrA˙ site, and consequently enhancing the ECL performance of the Ru(bpy)32+/TPrA system. Compared with single-valence MOFs and their physically mixed MOF composites, the ECL performance of the Ru@Ce (III,IV)-MOF/TPrA system is significantly improved, with the maximum ECL signal amplification factor reaching up to 2.7 times. The sensor developed using this strategy showed a broad linear range (1 × 10-4 ng mL-1-100 ng mL-1) and a low detection limit (0.036 pg mL-1), together with excellent selectivity, stability, and reproducibility. This strategy provides a promising route for constructing multifunctional MOF-based ECL sensors for sensitive tumor biomarker detection.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149219700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A self-contained ternary probe assay integrating self-priming cascade amplification with CRISPR/Cas12a for ultrasensitive detection of infant UTI-related microRNA. 一种集成自引级联扩增和CRISPR/Cas12a的独立三元探针检测方法，用于超灵敏检测婴儿尿路相关的microRNA。

IF 3 3区化学

Analytical Methods Pub Date : 2026-10-19 DOI: 10.1039/d6ay01892k

Guoxian Deng, Xiaowen Xia, Xianping Li, Jie Guo, Guo Wei

{"title":"A self-contained ternary probe assay integrating self-priming cascade amplification with CRISPR/Cas12a for ultrasensitive detection of infant UTI-related microRNA.","authors":"Guoxian Deng, Xiaowen Xia, Xianping Li, Jie Guo, Guo Wei","doi":"10.1039/d6ay01892k","DOIUrl":"https://doi.org/10.1039/d6ay01892k","url":null,"abstract":"Accurate quantification of infant urinary tract infection (UTI)-associated microRNAs remains challenging due to their short length, low abundance, and high sequence homology. Herein, we present a sensitive fluorescent biosensor that integrates a self-priming extension cascade with CRISPR/Cas12a-mediated signal amplification, with the core innovation being a rationally designed ternary sensing probe that consolidates all recognition and amplification elements into a single, self-contained architecture on magnetic beads. This integrated design eliminates the need for multiple exogenously added primers, offering three inherent advantages: fixed stoichiometric ratios ensure reproducible reaction initiation, the unified format simplifies the operational workflow, and stringent coupling of amplification to target recognition suppresses non-specific background. Upon target recognition, the probe undergoes programmed conformational rearrangements that trigger sequential self-priming extension events, simultaneously generating fragments that drive autocatalytic feedback and T7 promoter-containing products for downstream crRNA transcription. The transcribed crRNA activates Cas12a collateral cleavage, producing exponentially amplified fluorescence. Under optimized conditions, the assay achieves a detection limit of 0.48 fM, excellent single-base discrimination, and reliable performance in human serum. Quantification results correlate strongly with qRT-PCR. This exogenously primer-free, self-contained cascade uniquely unifies signal amplification, target regeneration, and crRNA synthesis in a single probe format, while the seamless coupling with CRISPR/Cas12a trans-cleavage ensures both ultrahigh sensitivity and exceptional specificity. The proposed strategy provides a promising platform for early diagnosis and screening of infant UTI.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149219646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective and sensitive colorimetric detection of copper(II) using methylthiazole-quinolinol based sensor: application in real samples and its experimental and DFT studies. 甲基噻唑-喹啉醇基传感器选择性灵敏比色法检测铜（II）：在实际样品中的应用及其实验和DFT研究。

IF 3 3区化学

Analytical Methods Pub Date : 2026-10-16 DOI: 10.1039/d6ay01732k

Sareh Sadat Abolghasemi, Hadi Fallah Moafi, Seyed Hossein Mousavi

{"title":"Selective and sensitive colorimetric detection of copper(II) using methylthiazole-quinolinol based sensor: application in real samples and its experimental and DFT studies.","authors":"Sareh Sadat Abolghasemi, Hadi Fallah Moafi, Seyed Hossein Mousavi","doi":"10.1039/d6ay01732k","DOIUrl":"https://doi.org/10.1039/d6ay01732k","url":null,"abstract":"A novel methylthiazole-quinolinol-based sensor (MTAMQ) named (E)-2-methyl-5-((4-methylthiazol-2-yl)diazenyl)quinolin-8-ol was synthesized. In the presence of copper(II) ions (Cu2+), the MTAMQ sensor exhibits a stable color change from yellow to red. The MTAMQ sensor displayed a linear response toward Cu2+ ions over the concentration range of 0.10 to 55 µM (0.006 to 3.5 mg L-1), with a detection limit of 103.93 nM (6.60 µg L-1). The binding constant of the MTAMQ-Cu2+ complex was 1.41 × 104 M-1, and the stoichiometric ratio of complex formation was 1 : 1. Using EDTA, the MTAMQ sensor can be recycled and reused up to 4 times. Density functional theory (DFT) calculations were conducted for the MTAMQ sensor and the MTAMQ-Cu2+ complex. The MTAMQ sensor was successfully used to detect and quantify Cu2+ ions in various foodstuff and water samples, and the results were very close to those of the atomic absorption spectroscopy (AAS) method. To enable the detection of Cu2+ ions without the need for a complex device, paper test strips were prepared using the MTAMQ sensor and offered successful results. The unique features of the MTAMQ sensor, including naked-eye detection of copper ions in environmental samples without the need for specialized equipment, real-time analysis, cost-effectiveness, simple operation, rapid response, high stability under different conditions, and excellent selectivity and sensitivity, make it a promising alternative to previously reported colorimetric sensors.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149190187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0