Anahid Rezaeefar, Mir Ali Farajzadeh, Mahboob Nemati, Mohammad Reza Afshar Mogaddam and Farzaneh Lotfipour
{"title":"Retraction: Application of new N- and S-doped amorphous carbon in D-μSPE and its combination with deep eutectic solvent-based DLLME for the extraction of some mycotoxins from soymilk","authors":"Anahid Rezaeefar, Mir Ali Farajzadeh, Mahboob Nemati, Mohammad Reza Afshar Mogaddam and Farzaneh Lotfipour","doi":"10.1039/D3AY90109B","DOIUrl":"10.1039/D3AY90109B","url":null,"abstract":"<p >Retraction of ‘Application of new N- and S-doped amorphous carbon in D-μSPE and its combination with deep eutectic solvent-based DLLME for the extraction of some mycotoxins from soymilk’ by Anahid Rezaeefar <em>et al.</em>, <em>Anal. Methods</em>, 2021, <strong>13</strong>, 4604–4613, https://doi.org/10.1039/D1AY01057C.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 9","pages":" 2224-2224"},"PeriodicalIF":2.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ay/d3ay90109b?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hasanain Najm Abed Altuwaijari, Mohammad Reza Afshar Mogaddam, Mir Ali Farajzadeh and Saeed Mohammad Sorouraddin
{"title":"In situ sorbent formation dispersive solid phase extraction for multi-elemental analysis: a new approach using cyclen and a magnetic ionic liquid†","authors":"Hasanain Najm Abed Altuwaijari, Mohammad Reza Afshar Mogaddam, Mir Ali Farajzadeh and Saeed Mohammad Sorouraddin","doi":"10.1039/D4AY01111B","DOIUrl":"10.1039/D4AY01111B","url":null,"abstract":"<p >This study introduces an <em>in situ</em> sorbent formation dispersive solid-phase extraction method for the simultaneous extraction of seven metal ions (V, Cr, Ni, Cu, Zn, Cd, and Pb) in petroleum samples. For this purpose, a home-made extraction device was used to perform the method on a relatively high volume of sample solution. The method employed cyclen as both a sorbent and a chelator. Also, a magnetic ionic liquid was used as the collection/dissolution solvent. Rigorous optimization yielded a wide linear range, low detection limits, good precision (relative standard deviations ≤8.5%), and high extraction recoveries (56–72%). The method's performance was validated in real petroleum samples from Iraq and Iran, demonstrating its applicability, accuracy, and robustness against matrix effects. The method was successfully utilized for the determination of target metal ions in real samples.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 10","pages":" 2273-2281"},"PeriodicalIF":2.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sean dos Santos Araújo, Wilson Tiago Fonseca, Maria Fátima das Graças Fernandes da Silva, Moacir Rossi Forim, João Batista Fernandes and Ronaldo Censi Faria
{"title":"Disposable electrochemical biosensor based on acetylcholinesterase for inhibition assays using a natural substance and plant extracts†","authors":"Sean dos Santos Araújo, Wilson Tiago Fonseca, Maria Fátima das Graças Fernandes da Silva, Moacir Rossi Forim, João Batista Fernandes and Ronaldo Censi Faria","doi":"10.1039/D4AY02084G","DOIUrl":"10.1039/D4AY02084G","url":null,"abstract":"<p >In general, insects are considered pests in agricultural areas, and their control is essential for high productivity of the cultivated areas. Control of these insects can be achieved by the inhibition of enzymes present in the insect's body. The enzyme acetylcholinesterase (AChE) is present in the neuromuscular junctions of vertebrates and invertebrates, and it is an important target for pest control. Herein, we present the development of a disposable electrochemical biosensor based on AChE (Bio-AChE) to rapidly evaluate the presence of new potential inhibitors in crude extracts of plants. Bio-AChE was constructed by modifying the disposable screen-printed carbon electrode (SPCE) with glutathione-decorated gold nanoparticles on which AChE was covalently immobilized. Electrochemical studies confirm the effective immobilization of the enzyme, and the Bio-AChE was applied to assess the inhibitory activity of azadirachtin, obtained from <em>Azadirachta indica</em>, which is a well-known AChE inhibitor. The proposed biosensor showed excellent results, demonstrating the inhibition activity of azadirachtin against AChE. The crude extracts of <em>Picramnia riedelli</em>, <em>P. ciliata</em>, and <em>Toona ciliata</em> were evaluated with the Bio-AChE, and all showed inhibition percentage values of around 50%. The extracts were evaluated by <small><sup>1</sup></small>H NMR spectra, which identified classes of natural compounds that could be responsible for the inhibition activity. The proposed disposable Bio-AChE was shown to be a reliable method for simple and rapid screening of new inhibitors in plant extracts, opening an avenue for the screening of new natural products with potential for pest control.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 11","pages":" 2417-2426"},"PeriodicalIF":2.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maarten Dill, Sophia Barhdadi, Celine Vanhee and Eric Deconinck
{"title":"Sample preparation methods for elemental analysis in electronic cigarette aerosols: a critical review","authors":"Maarten Dill, Sophia Barhdadi, Celine Vanhee and Eric Deconinck","doi":"10.1039/D4AY02127D","DOIUrl":"10.1039/D4AY02127D","url":null,"abstract":"<p >In the last decade, e-cigarettes have been marketed as a less harmful alternative to classical tobacco smoking and are currently being used by millions of people. An e-cigarette consists of an e-liquid and a heating device, generating an aerosol that the user can inhale. Recently, several studies have shown that metals and metalloids, including As, Cd, and Pb, known carcinogens, were present in these aerosols. To ensure the accuracy of dose-toxicity estimations, it is essential to have access to reliable and reproducible methods for estimating the dose in question. Although more standardization methodologies were introduced in the analysis of metal(loid)s from aerosols, a huge divergence in sample preparation can be found in the literature. This work aimed to provide an overview of the sample preparation methods used to quantify these metal(loid)s. Therefore, a literature search was conducted in September 2024, that followed the PRISMA guidelines. Among the 48 articles selected, a wide variability in sample preparation was observed, specifically variations in aerosol generation characteristics and collection techniques. Despite the widespread use of methods as filters and impingers, many studies failed to validate critical steps such as aerosol recovery, blank corrections, and the extent of matrix effects. In addition, poor inorganic methodological practices were often applied, such as excessive use of glass materials for aerosol collection or other sample preparation steps. Therefore, further standardization of methodologies is urgently needed to improve the reliability of metal quantification in e-cigarette aerosols, which could potentially enhance regulatory frameworks and facilitate the routine analysis of e-cigarette emissions.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 9","pages":" 1997-2014"},"PeriodicalIF":2.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Duo Zhang, Hongyi Bai, Laijun Sun, Zibo Han and Shihao Zhang
{"title":"Ultraviolet spectral transfer based on a convolutional variational autoencoder model for detecting chemical oxygen demand in rivers","authors":"Duo Zhang, Hongyi Bai, Laijun Sun, Zibo Han and Shihao Zhang","doi":"10.1039/D4AY02052A","DOIUrl":"10.1039/D4AY02052A","url":null,"abstract":"<p >The spectral data shift generated using two different ultraviolet (UV) instruments to detect chemical oxygen demand (COD) in rivers often invalidates existing predictive models. To solve this problem, this study proposes a non-linear spectral transfer method based on a convolutional variational autoencoder (CVAE). This method extracts key features from spectral data using an encoder and reconstructs the original data using a decoder, which significantly reduces the impact of spectral shifts caused by differences in the resolution of measurement devices. Specifically, the encoder of the CVAE combines the convolutional layers with a sigmoid activation function to effectively capture the non-linear relationship between the spectra of the master and slave devices. Furthermore, in the face of the problem that traditional autoencoders lack the modeling ability for data distribution, this method adopts the idea of variational inference to learn the potential representation of COD spectral signals, thereby effectively capturing the global features of the COD spectral signals. Finally, we input the transformation spectra derived from the spectral space transformation, piecewise direct standardization, deep autoencoders, convolutional autoencoder, and the proposed method into the partial least squares multivariate calibration model. The results show that the proposed method reduces the root mean square error of the prediction by 1.0242, 0.4571, 0.4201, and 0.1965, respectively, compared to the other four methods. The above results indicate that the proposed method effectively solves the problem of model sharing between different instruments and provides new perspectives for research and applications in related fields.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 11","pages":" 2446-2455"},"PeriodicalIF":2.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yihao Wang, Xiaoou Han, Jiaqing Wang, Hongling Wang, Rong Yan and Na Yu
{"title":"Comparative metabolomic in-depth exploration of red raspberry: new insights into changes in phytochemicals between different breeds","authors":"Yihao Wang, Xiaoou Han, Jiaqing Wang, Hongling Wang, Rong Yan and Na Yu","doi":"10.1039/D4AY01988A","DOIUrl":"10.1039/D4AY01988A","url":null,"abstract":"<p >Based on a non-targeted metabolomic approach, a comprehensive analysis of the metabolic profiles of two raspberry cultivars, Selina (TSM) and Tulameen (CSM), was conducted to explore the potential for further processing of these two varieties. A total of 752 metabolites were identified in both raspberry varieties, with 375 metabolites detected in negative ion mode and additional metabolites identified in positive ion mode. The total number of differential metabolites between TSM and CSM was 62, with orthogonal partial least squares discriminant analysis (OPLS-DA) revealing that the main differential metabolites between the two varieties were phenolic compounds, organic acids, and alkaloids. KEGG pathway analysis identified the top three significantly different metabolic pathways as biosynthesis of amino acids; valine, leucine, and isoleucine biosynthesis; and alanine, aspartate, and glutamate metabolism. In conclusion, this study comprehensively analyzed the metabolic characteristics of TSM and CSM raspberry varieties, providing valuable insights and revealing their potential application prospects. Compared to previous studies, this research provides new insights into the metabolic differences between TSM and CSM cultivars, specifically highlighting newly identified metabolites that were not reported before. These findings include novel differences in phenolic compounds and alkaloids, which play important roles in plant stress response and have potential health benefits. This enhances our understanding of the unique properties of each cultivar and underscores the biological functions of the newly identified metabolites, contributing to the broader field of functional food development.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 9","pages":" 2112-2124"},"PeriodicalIF":2.7,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yen Hai Tran, Trang Thuy Dao, Ung Dinh Nguyen, Thien Ba Tran, Loi Phuc Luu, Huy Quang Duong and Tho H. Ho
{"title":"Sensitive detection of circulating methylated SEPT9 in hepatocellular carcinoma patients using a novel quantitative PCR assay†","authors":"Yen Hai Tran, Trang Thuy Dao, Ung Dinh Nguyen, Thien Ba Tran, Loi Phuc Luu, Huy Quang Duong and Tho H. Ho","doi":"10.1039/D4AY02168A","DOIUrl":"10.1039/D4AY02168A","url":null,"abstract":"<p >Hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths worldwide. Early detection is crucial, yet reliable biomarkers are limited. Methylated <em>SEPT9</em> (m<em>SEPT9</em>) has emerged as a promising biomarker for HCC. Building upon previous ExBP technology, we enhanced the semi-nested realtime PCR assay by integrating TaqMan probes, enabling quantitative detection of mSEPT9 in plasma samples of HCC patients. The assay was validated using synthetic DNA standards and plasma samples from 49 HCC patients, 20 chronic liver disease (CLD) patients, and 32 healthy donors (HDs). Our assay demonstrated sensitivity in detecting methylation ratios as low as 1 : 100 000. The assay showed a strong linear correlation between Ct values and methylation levels over four orders of magnitude (<em>R</em><small><sup>2</sup></small> = 0.96178), indicating robust quantification. Clinically, the assay revealed significant differences in ΔCt values between HCC patients (median ΔCt = 19.55) and controls (CLD: 29.32 and HDs: 26.19, <em>p</em> < 0.005). ROC analysis for HCC <em>vs.</em> controls yielded an AUC of 0.729, with 77.55% sensitivity and 59.62% specificity at the optimal cutoff (≤25.98). Notably, the assay identified 72.73% of HCC cases with AFP levels below 20 ng mL<small><sup>−1</sup></small>, underscoring its potential in detecting AFP-negative cases. These findings suggest that the novel m<em>SEPT9</em> assay is a sensitive and specific tool for early HCC detection, offering prognostic value for clinical monitoring.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 9","pages":" 2181-2190"},"PeriodicalIF":2.7,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ay/d4ay02168a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Impact of mechanical calibration variations on USP prednisone PVT with standard and apex vessels†","authors":"Ethan J. Pozy and Li Tian","doi":"10.1039/D4AY02008A","DOIUrl":"10.1039/D4AY02008A","url":null,"abstract":"<p >Dissolution testing is a method to assess the impact of pharmaceutical formulation and bioequivalence between comparator drugs, among other uses. The dissolution paddle tester (USP Apparatus 2) has been widely used by industry to evaluate the rate of a drug's dissolution from a formulation. The United States Pharmacopeia (USP) issued a new Dissolution Performance Verification Standard (DPVS) – Prednisone Reference Standard in 2023, along with a new set of Performance Verification Test (PVT) criteria to be performed in addition to mechanical calibration for instrument qualification. Apex vessels are dissolution vessels with a convex bottom that have gained interest due to improved hydrodynamics and reduced accumulation of trapped drug product in formulation at the bottom of the vessel during dissolution testing. In this study, we evaluated the effect of changing mechanical calibration parameters within the acceptable mechanical calibration ranges and their impacts on the dissolution of the new DPVS – Prednisone tablets in deaerated water using both standard and apex vessels as well as whether the test results from varied apparatus configurations met the PVT acceptance criteria. All configurations within specified parameters for standard vessels fell within PVT acceptance criteria, but the higher dissolution rate in apex vessels fell beyond the current PVT acceptance range. This indicates that the current PVT or DPVS-Prednisone tablet is not suitable for the qualification of USP Apparatus 2 fitted with apex vessels.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 12","pages":" 2559-2566"},"PeriodicalIF":2.7,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ghizlène Boudghene Stambouli, Belkacem Benguella, Benamar Makhoukhi, Mohamed Said El-ouchdi and Ayman H. Kamel
{"title":"Physicochemical properties of mesoporous acid activated materials from Lemna minor for Bezaktiv Red S-MAX dye removal","authors":"Ghizlène Boudghene Stambouli, Belkacem Benguella, Benamar Makhoukhi, Mohamed Said El-ouchdi and Ayman H. Kamel","doi":"10.1039/D4AY02206H","DOIUrl":"10.1039/D4AY02206H","url":null,"abstract":"<p > <em>Lemna minor</em>, a cheap and easily accessible substance, was utilized to create activated carbon through acid activation [with H<small><sub>3</sub></small>PO<small><sub>4</sub></small> (ALM-P) and citric acid (ALM-C)]. Brunauer–Emmett–Teller (BET) analysis reveals that the <em>L. minor</em> activated materials (LACs) had a surface area of 208.54 m<small><sup>2</sup></small> g<small><sup>−1</sup></small> and 216.11 m<small><sup>2</sup></small> g<small><sup>−1</sup></small> after H<small><sub>3</sub></small>PO<small><sub>4</sub></small> and citric acid activation, respectively. The presence of rich hydroxyl, carboxyl, amide, and phosphate functional groups on the surface of LACs is revealed by Fourier transform infrared spectroscopy (FTIR). This enables easy Bezaktiv dye, type Red S-MAX (BRSM), binding to the surface through strong chemisorptive bonds or ion exchange. With a maximal mono-layer adsorption capacity (<em>q</em><small><sub>m</sub></small>) of 16.86 and 7.69 mg g<small><sup>−1</sup></small> at 25 °C, the pseudo-second-order model and Langmuir isotherm provided a good description of the kinetic and equilibrium data. The adsorption was caused in part by the intra-particle diffusion mechanism. With negative Δ<em>G</em>° and positive Δ<em>H</em>° values, the adsorption process was spontaneous and endothermic. Under optimum conditions of pH 3.0 and 25 °C, the maximum % removal for BRSM (90 mg L<small><sup>−1</sup></small>) was reported to be 77.65% within 90 min. These indicated that dye-contaminated water might be treated with the inexpensive LACs as a possible adsorbent.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 9","pages":" 2134-2143"},"PeriodicalIF":2.7,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Auraya Manaprasertsak, Robin Rydbergh, Qicheng Wu, Maria Slyusarenko, Christopher Carroll, Sarah R. Amend, Sofie Mohlin, Kenneth J. Pienta, Per Malmberg and Emma U. Hammarlund
{"title":"Chemical profiling of surviving cancer cells using ToF-SIMS and MCR analysis discriminates cell components†","authors":"Auraya Manaprasertsak, Robin Rydbergh, Qicheng Wu, Maria Slyusarenko, Christopher Carroll, Sarah R. Amend, Sofie Mohlin, Kenneth J. Pienta, Per Malmberg and Emma U. Hammarlund","doi":"10.1039/D4AY02238F","DOIUrl":"10.1039/D4AY02238F","url":null,"abstract":"<p >Cancer cells survive treatment through mechanisms that remain unclear. This study investigates the chemical changes that occur in cancer cells after treatment, focusing on lipid metabolism as a potential marker for survival and resistance. Using Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) and advanced multivariate statistical analysis, we compared the chemical profiles of untreated and surviving cancer cells. Region-of-Interest (ROI) analysis revealed distinct differences in the lipid compartments, with surviving cancer cells showing significant accumulation of lipid droplets. While Principal Component Analysis (PCA) was able to differentiate the chemistry of untreated and surviving cancer cells as well as their cellular components, Multivariate Curve Resolution (MCR) provided a clearer and more detailed distinction, enabling the identification of specific cellular features such as the cytoplasm, nucleus, and lipid droplets within the surviving cells. The separation of the chemistry in nucleus and lipid droplets emphasizes the effectiveness in complex spectral analysis. Furthermore, the ability to map the distribution of lipid droplets in surviving cells can advance our understanding of how these structures contribute to cancer cell survival during treatment. The study highlights the importance of lipid droplets as potential biomarkers for cancer cell adaptation and survival post-treatment, with implications for developing new therapeutic strategies.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 10","pages":" 2263-2272"},"PeriodicalIF":2.7,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ay/d4ay02238f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}