Analytical Methods最新文献_第9页

Cost-effective quantification of uric acid using niobium oxide and graphene oxide-modified pencil-drawn electrodes on PVC substrates† 使用聚氯乙烯基底上的氧化铌和氧化石墨烯改性铅笔绘制电极进行尿酸定量分析，具有成本效益。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-09-30 DOI: 10.1039/D4AY01345J

Douglas P. M. Saraiva, Bruno Ferreira, Leonardo M. A. Ribeiro, Thiago R. L. C. Paixão and Mauro Bertotti

{"title":"Cost-effective quantification of uric acid using niobium oxide and graphene oxide-modified pencil-drawn electrodes on PVC substrates†","authors":"Douglas P. M. Saraiva, Bruno Ferreira, Leonardo M. A. Ribeiro, Thiago R. L. C. Paixão and Mauro Bertotti","doi":"10.1039/D4AY01345J","DOIUrl":"10.1039/D4AY01345J","url":null,"abstract":"This study introduces a cost-effective approach for quantifying uric acid (UA), the main antioxidant species in human physiology and implicated in inflammatory regulation. Using a PVC substrate and pencil drawing technique, electrodes were fabricated and modified with niobium oxide and graphene oxide via a straightforward “drop casting” method. The nanostructures of the substrate, electrode, and modified electrode were evaluated using SEM images. The synergistic effect between these materials significantly facilitated the uric acid oxidation process with a 400 mV peak potential shift and 45% current increase. The evaluation of the electrode's response to common blood and urine components showed minimal deviation. Among the components tested—ascorbic acid, glucose, nitrate, nitrite, cysteine, urea, creatinine, and ammonium ion—only the ammonium ion exhibited a 10% interference at concentrations commonly found in urine. The sensors showed a good detection limit of 8.7 μmol L−1, with a wide linear range from 8.7 to 2000 μmol L−1 with a correlation factor of 0.9993 for five different sensors. The reproducibility and repeatability of the produced sensors were estimated by the RSD at 4% and 1%, respectively. Synthetic urine samples spiked exhibited reliable analysis, with recovery values within a 5% error margin. This work presents a practical, simple, and affordable sensor platform for rapid and accurate UA quantification.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142363518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in the CRISPR/Cas system-based visual detection method 基于 CRISPR/Cas 系统的视觉检测方法的最新进展。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-09-30 DOI: 10.1039/D4AY01147C

Jinrong Chen, Hang Su, June Hyun Kim, Lishang Liu and Rui Liu

引用次数: 0

A new filtration system for extraction and accurate quantification of microplastics† 用于提取和准确量化微塑料的新型过滤系统。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-09-30 DOI: 10.1039/D4AY01276C

JuHye Kim, Jihye Kwon, JeongHyun Kwon, Muhammad Zain Siddiqui, Gijeong Woo, Mijung Choi, Sungguan Hong, Chuan Ma, Shogo Kumagai, Atsushi Watanabe, Norio Teramae, Su Shiung Lam and Young-Min Kim

{"title":"A new filtration system for extraction and accurate quantification of microplastics†","authors":"JuHye Kim, Jihye Kwon, JeongHyun Kwon, Muhammad Zain Siddiqui, Gijeong Woo, Mijung Choi, Sungguan Hong, Chuan Ma, Shogo Kumagai, Atsushi Watanabe, Norio Teramae, Su Shiung Lam and Young-Min Kim","doi":"10.1039/D4AY01276C","DOIUrl":"10.1039/D4AY01276C","url":null,"abstract":"The analysis of microplastics is crucial due to their widespread occurrence and significant impact as environmental pollutants. Appropriate extraction and analytical techniques are necessary to evaluate the abundance, dispersal, and effects of microplastics. In this context, the filtration step to extract microplastics from solution media is indispensable for quantifying microplastics accurately. Usually, a pretreatment procedure such as density separation or solvent extraction is employed before filtration. Nevertheless, the adsorption of microplastic particles onto the glassware surface is known to occur and careful rinsing is inevitable for a suitable recovery rate. The study presents a novel filtration device developed for easy use to increase microplastic recovery from liquid samples. Fluorescent polyethylene (PE) particles were used to examine recovery by optical microscopy and pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS). The conventional filtration system (CFS) exhibited lower microplastic recovery (45.1 ± 7.9%, n = 10) due to the adsorption of microplastics onto the inner surface and the bottom edge of the upper glass funnel. In contrast, the newly developed modified filtration system (MFS) enables fast suction of liquid samples by removing the glass frit with the upper glass funnel and filter being spatially separated. The significantly improved recovery rates using MFS for PE particles were 100% (n = 5) and 93.4 ± 3.4% (n = 10), respectively, confirming the feasibility of the newly developed filtration system to analyze microplastics using Py-GC/MS. The study highlights the importance of filtration processing in establishing analytical standards for assessing and managing microplastics by suggesting MFS as an advanced filtration system.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142337421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct determination of lithium in brine samples using handheld LIBS without sample treatment: sample introduction by venturi system 使用手持式 LIBS 直接测定盐水样品中的锂，无需样品处理：通过文丘里管系统引入样品。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-09-27 DOI: 10.1039/D3AY02025H

Nayadet Erbetta, Gonzalo Puebla, Dave Day, Morgan Jennings, Agustin Loureiro, Christopher Green, Loverchio Gallardo and Waldo Quiroz

{"title":"Direct determination of lithium in brine samples using handheld LIBS without sample treatment: sample introduction by venturi system","authors":"Nayadet Erbetta, Gonzalo Puebla, Dave Day, Morgan Jennings, Agustin Loureiro, Christopher Green, Loverchio Gallardo and Waldo Quiroz","doi":"10.1039/D3AY02025H","DOIUrl":"10.1039/D3AY02025H","url":null,"abstract":"Splashing is the main problem for the direct analysis of aqueous samples using LIBS since it generates serious precision and accuracy issues. This study demonstrates the direct determination of Li content in brines for the control of industrial mining processes using a portable LIBS device based on the direct laser impact on the sample, without any sample treatment, through the design of a sample injection system based on the Venturi effect. Our results demonstrated that the utilization of the 653.3 nm hydrogen line as an internal standard reduces the model calibration fitting error from 0.440 root mean square error in a standard calibration curve to 0.123 on the internal standard curve. Conversely, the development of a Venturi effect-based injection device using compressed air converts the brine into a fine mist which decrease splashing, resulting in an up to 10-fold error reduction, all without the necessity of employing an internal standard. Our results, evaluated by comparing them to the ASTM D3561-11 standard method using flame atomic absorption spectrometry, indicate that it is feasible to determine the lithium content in brine samples with an error of under 20% and a detection limit of 13 mg kg−1.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/ay/d3ay02025h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142337422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid determination of 54 dye components in hair dyes by liquid chromatography coupled to quadrupole orbitrap high-resolution mass spectrometry† 液相色谱-四极杆轨道rap高分辨质谱法快速测定染发剂中的 54 种染料成分。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-09-27 DOI: 10.1039/D4AY00981A

Jing Sun, Gao-Xu Xue, Xu Gong, Zai-Ping Zhang, Jie Xu, Lei Chen, Ling Cao, You-Long Feng and Yun-Jing Zhang

{"title":"Rapid determination of 54 dye components in hair dyes by liquid chromatography coupled to quadrupole orbitrap high-resolution mass spectrometry†","authors":"Jing Sun, Gao-Xu Xue, Xu Gong, Zai-Ping Zhang, Jie Xu, Lei Chen, Ling Cao, You-Long Feng and Yun-Jing Zhang","doi":"10.1039/D4AY00981A","DOIUrl":"10.1039/D4AY00981A","url":null,"abstract":"Chemical hair dye components can have allergenic, reproductive, and carcinogenic risks. Detecting restricted and prohibited ingredients in these products is challenging due to product diversity, isomer separation, instability, and wide polarity range. A method was developed using HPLC-high-resolution mass spectrometry for the qualitative and quantitative analysis of 54 hair dye components in various products. Samples were extracted with a 70% methanol solution, ultrasonicated in an ice bath, centrifuged, filtered, diluted with 25% methanol solution and 25% methanol solution containing 0.05% D-isoascorbic acid. Separation was achieved using an ACE Excel 3 C18 column (2.1 mm × 150 mm, 3 μm) with analysis conducted via quadrupole Orbitrap mass spectrometry. It showed good linear correlations, with detection limits of 0.1–23.5 ng mL−1, and quantitation limits of 0.2–78.1 ng mL−1. Average recovery ranged from 60.0% to 118.4%, with repeatability from 4.0% to 14.9%. Stability was confirmed within 48 hours. When applied to 20 batches of commercially available hair dyes, 24 hair dye components were found within permissible levels. The method is crucial for quality control of hair dyes, covering 10 common prohibited and 44 permissible hair dye components outlined in the Safety and Technical Standards for Cosmetics (2015 Edition). Compared to the standard methods, it can separate isomers in a single mobile phase system within 15 minutes in positive ion mode while maintaining sensitivity for phenol, hydroquinone, and other components in negative ion mode. Moreover, the pre-treatment strategy significantly improved stability and accuracy, enabling precise analysis of the 54 hair dye components.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142337426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paper-based colorimetric sensor using a single-atom nanozyme for the ultrasensitive detection of Cr(vi) in short-necked clams† 利用单原子纳米酶的纸基比色传感器超灵敏检测短颈蛤中的六（Cr）。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-09-27 DOI: 10.1039/D4AY00891J

Shuo Wu, Muhammad Arif Khan, Lifan Zhang, Hongbin Zhao, Tianzeng Huang, Hongmei Cao and Daixin Ye

{"title":"Paper-based colorimetric sensor using a single-atom nanozyme for the ultrasensitive detection of Cr(vi) in short-necked clams†","authors":"Shuo Wu, Muhammad Arif Khan, Lifan Zhang, Hongbin Zhao, Tianzeng Huang, Hongmei Cao and Daixin Ye","doi":"10.1039/D4AY00891J","DOIUrl":"10.1039/D4AY00891J","url":null,"abstract":"Single-atom nanozymes (SAzymes) as a class of highly active nanozymes with the advantages of high atom utilization, high catalytic activity and stability have attracted great attention. In this work, Fe–N–C SAzymes with exceptional oxidase (OXD)-like activity were achieved utilizing polyvinylpyrrolidone (PVP) as a template. The Fe–N–C SAzymes with remarkable OXD-like activity could oxidize TMB to blue oxTMB, but 8-hydroxyquinoline (8-HQ) as a metal chelator is capable of discoloring oxTMB. Thus, the addition of 8-HQ decolorized the solution. However, upon the introduction of Cr(VI) ions, 8-HQ preferentially chelated with the Cr(VI) ions, reversing the inhibition of the color reaction and restoring the blue color. Based on this phenomenon, we constructed a novel paper-based analytical device (PAD) that exhibited a linear range of 5–1000 μM and an LOD of 1.2 μM. Importantly, the PAD used in this study shows the merits of simplicity, low preparation costs, and rapid reaction times. When combined with smartphone RGB analysis, it enables the simultaneous analysis of eight different Cr(VI) concentrations without the need for large-scale instrumentation. Moreover, the proposed PAD displays high selectivity, accuracy and utility in testing actual short-necked clam samples. This work not only provides a simple and cost-effective method to detect Cr(VI) but also makes a contribution to rapid food testing.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142337425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell detection and linear discriminant analysis of bacterial Raman spectra in glass filter microholes 玻璃滤器微孔中细菌拉曼光谱的单细胞检测和线性判别分析。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-09-25 DOI: 10.1039/D4AY01272K

Daisuke Onoshima, Kentaro Uchida, Tomomine Iida, Takashi Kojima, Yukihiro Ikeda, Daijiro Iwata, Ikuo Nagasawa, Hiroshi Yukawa and Yoshinobu Baba

引用次数: 0

Label-free impedimetric analysis of microplastics dispersed in aqueous media polluted by Pb²⁺ ions. 对分散在受 Pb2+ 离子污染的水介质中的微塑料进行无标签阻抗分析。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-09-25 DOI: 10.1039/d4ay01324g

Davide Lascari, Salvatore Cataldo, Nicola Muratore, Giuseppe Prestopino, Bruno Pignataro, Giuseppe Lazzara, Giuseppe Arrabito, Alberto Pettignano

{"title":"Label-free impedimetric analysis of microplastics dispersed in aqueous media polluted by Pb2+ ions.","authors":"Davide Lascari, Salvatore Cataldo, Nicola Muratore, Giuseppe Prestopino, Bruno Pignataro, Giuseppe Lazzara, Giuseppe Arrabito, Alberto Pettignano","doi":"10.1039/d4ay01324g","DOIUrl":"https://doi.org/10.1039/d4ay01324g","url":null,"abstract":"The rapid differentiation between polluted and unpolluted microplastics (MPs) is critical for tracking their presence in the environment and underpinning their potential risks to humans. However, the quantitative analysis of polluted microplastics on the field is limited by the lack of rapid methods that do not need optical analysis nor their capture onto sophisticated electrochemical sensor platforms. Herein, a simple analytical approach for MPs dispersed in aqueous media leveraging electrochemical impedance spectroscopy (EIS) analysis on screen-printed sensors is presented. This method is demonstrated by the EIS-based analysis of two standards of microplastics beads (MPs), one of polystyrene (PS) and one of polystyrene carboxylated (PS-COOH), when exposed to aqueous solutions containing Pb2+ ions. The adsorption of Pb2+ ions on the MPs was quantitatively determined by voltammetric analysis. EIS permitted to rapidly (about 2 minutes) differentiate clean MPs from the Pb2+ polluted ones. These results could constitute a first-step towards the realization of a portable impedimetric sensor for the quantification of microplastics polluted by metal ions in aqueous solutions.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142337424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined detection of hepatitis B virus surface antigen and hepatitis B virus DNA using a DNA sensor† 使用 DNA 传感器联合检测乙型肝炎病毒表面抗原和乙型肝炎病毒 DNA。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-09-24 DOI: 10.1039/D4AY01629G

Hang Gong, Shufen Yao, Yong Li, Chunyan Chen, Feng Chen and Changqun Cai

{"title":"Combined detection of hepatitis B virus surface antigen and hepatitis B virus DNA using a DNA sensor†","authors":"Hang Gong, Shufen Yao, Yong Li, Chunyan Chen, Feng Chen and Changqun Cai","doi":"10.1039/D4AY01629G","DOIUrl":"10.1039/D4AY01629G","url":null,"abstract":"Hepatitis B virus (HBV) infection is associated with the progression of liver disease. Occult HBV infection (OBI) is defined as the existence of detectable HBV DNA in HBV surface antigen negative individuals. However, HBV DNA is negative in serum while HBV surface antigen remains positive in incompletely cured chronic HBV infection. Hence, combined detection of HBV surface antigen and HBV DNA is essential for the accurate detection and rehabilitation of HBV infection. Therefore, a multiplex detection strategy based on branched DNA nanostructures was developed. The single-stranded segment of a branched DNA nanostructure (segments of S4 and S2) assembled by four single-stranded DNA was hybridized with the aptamer of HBV surface antigen and DNA hairpin to construct a DNA nanosensor, which can achieve high specificity identification and highly sensitive fluorescence responses to the targets. The detection limits of the developed nanosensor for HBV and HBV DNA are 50 pM and 5 nM, respectively. The combined detection of HBV surface antigen and HBV DNA provides a new insight for more thorough diagnostic evaluation of HBV infection and rehabilitation.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A colorimetric-aptamer-based assay for the determination of enrofloxacin through triggering the aggregation of gold nanoparticles† 通过触发金纳米粒子的聚合来测定恩诺沙星的比色-aptamer 分析法。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-09-23 DOI: 10.1039/D4AY01259C

Wei-Chuang Kong, Chen-Chen Li, Ai-Hong Zhang, Xin-Long Li, Qian-Rui Gong, Bing-Tan Jin, Xiao-Juan Jia, Xu-Ying Liu and Yan-Fei Kang

{"title":"A colorimetric-aptamer-based assay for the determination of enrofloxacin through triggering the aggregation of gold nanoparticles†","authors":"Wei-Chuang Kong, Chen-Chen Li, Ai-Hong Zhang, Xin-Long Li, Qian-Rui Gong, Bing-Tan Jin, Xiao-Juan Jia, Xu-Ying Liu and Yan-Fei Kang","doi":"10.1039/D4AY01259C","DOIUrl":"10.1039/D4AY01259C","url":null,"abstract":"Although enrofloxacin (ENR) is a widely used broad-spectrum antibiotic in veterinary medicine, its residues in animals can pose a risk to human health. Thus, we developed a new method for detecting ENR based on aptamers and AuNPs. In the absence of ENR, the aptamers attached to the surface of the AuNPs via electrostatic interactions to protect the AuNPs from NaCl, and the solution remained red. Conversely, the aptamer bonded with ENR, leading the aptamer to detach from the AuNP surface, and the color of the solution changed from red to blue. Based on this principle, ENR can be qualitatively detected by the naked eye and quantitatively detected by measuring the absorbance ratio at 650 nm and 530 nm. The experimental results showed a good linear relationship within the ENR concentration range of 0–400 nM, with a limit of detection (LOD) of 1.72 nM, which is satisfactory for detection in food safety. Additionally, this method has also been successfully applied to the detection of ENR in tap water, river water, milk, serum and urine, with good recovery rates and RSD values of less than 7%, indicating its great potential for ENR detection in environmental water samples. More importantly, the combination of this method with a smartphone platform provided great convenience for on-site and visual detection of ENR, offering promising applicability prospects.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142277386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0