A highly sensitive flow injection chemiluminescence method for myoglobin detection in beef samples based on zinc ion-induced “dragging” of heme iron†

Abstract

We report for the first time that zinc ions (Zn²⁺) significantly enhance the chemiluminescence (CL) signal of the luminol–hydrogen peroxide (luminol–H₂O₂) system by “dragging” heme iron out of myoglobin (Mb). Integrating this with myoglobin aptamer (Mb aptamer) specific recognition enables sensitive and specific quantification of Mb content in beef samples.