Wiley最新文献

{"title":"Plant Responses to Anomalous Heat and Drought Events in the Sonoran Desert","authors":"Benjamin T. Wilder,&nbsp;Kevin R. Hultine,&nbsp;Wetherbee Bryan Dorshow,&nbsp;Sula E. Vanderplank,&nbsp;Blanca R. López,&nbsp;Alfonso Medel-Narváez,&nbsp;Monica Marvan,&nbsp;Kristen Kindl,&nbsp;Aryn Musgrave,&nbsp;Shane Macfarlan,&nbsp;Exequiel Ezcurra","doi":"10.1111/gcb.70217","DOIUrl":"https://doi.org/10.1111/gcb.70217","url":null,"abstract":"<p>A shift to greater aridification in dry regions of the world is ongoing and rapidly increasing in intensity, including in the biodiverse Sonoran Desert of the Southwest United States and northern Mexico. In addition to experiencing over two decades of drought, the Sonoran Desert is facing anomalous heat events that are increasing in frequency, evidenced in a record hot and dry period from 2020 to 2021. This article evaluates the impacts of the 2020–2021 region-wide heat and drought event at three scales: (1) a landscape level assessment of ecosystem stress across the entirety of the Sonoran Desert based on precipitation and temperature data from meteorological stations and a satellite-derived vegetation health index (VHI), (2) assessments of stress on iconic columnar cacti and succulent trees, and (3) mechanistic plant responses to extreme heat and drought, and secondary biotic stressors from insect attacks. 2020 was the hottest and driest year since 1980 across the Sonoran Desert region, and vegetation health, determined from VHI, was also near its lowest point. Field-based assessments of columnar cacti across the Sonoran Desert revealed high levels of acute plant stress, including cactus scorching, defined by rapid onset of discolored photosynthetic tissue that leads to permanent photosynthetic dysfunction and increased plant mortality. Tissue scorching corresponded with a three-fold increase in mortality of giant cactus species across the region relative to background levels following 2020–2021. Likewise, repeated plant health surveys show a persistent legacy of the 2020–2021 anomaly, resulting in a marked reduction in the current health and survival of the iconic giant saguaro (<i>Carnegiea gigantea</i>) in the northern Sonoran Desert. This multi-scale assessment of previously anomalous heat and drought events on succulent desert plants shows landscape-wide impacts that could fundamentally reshape populations of these keystone species and the communities that depend on them.</p>","PeriodicalId":175,"journal":{"name":"Global Change Biology","volume":"31 5","pages":""},"PeriodicalIF":10.8,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gcb.70217","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neurogrit Gold Attenuates 6-OHDA-Induced Dopaminergic Neurodegeneration in Parkinson's Model of Caenorhabditis elegans by Reducing α-Synuclein Accumulation and Pink/Pdr-1 Driven Mitochondrial Dysfunction","authors":"Acharya Balkrishna,&nbsp;Nishit Pathak,&nbsp;Rani Singh,&nbsp;Vivek Gohel,&nbsp;Yash Varshney,&nbsp;Rishabh Dev,&nbsp;Anurag Varshney","doi":"10.1111/cns.70401","DOIUrl":"https://doi.org/10.1111/cns.70401","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Parkinson's disease (PD) is a neurodegenerative disorder majorly associated with movement and behavioral disturbances. Pathologically, the loss of dopaminergic (DA) neurons triggered by the deposition of α-synuclein (SNCA) leads to the decrease in dopamine levels affecting motor and cognitive functions of the brain. Current pharmacotherapy for PD only addresses its symptoms but is not able to halt its progression. Traditional medicines are being increasingly used for the treatment of neurodegenerative disorders.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>The present study investigated the effects of Neurogrit Gold (NG), a herbo-mineral prescription medicine, on a Parkinson's model of <i>Caenorhabditis elegans</i>.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Chemical characterization of NG was performed on HPLC and GC–MS/MS platforms. Evaluation of NG was done in the neurotoxicant 6-OHDA-induced N2, BZ555, and NL5901 strains of <i>C. elegans</i>.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>It was observed that NG treatment did not hamper the lifespan, survival, and progeny development of <i>C. elegans</i> strains. The worms treated with NG were able to resist the deleterious effects of 6-OHDA on survival, progeny development, body bends, and chemotaxis in N2 and DA neuron degeneration in BZ555 worms. In NL5901 worms, NG treatment reduced SNCA aggregation, restored lipid content, as well as improved body bends, chemotaxis, and food uptake. Gene expression studies on 6-OHDA exposed and NG-treated N2 worms suggest that the neuroprotective effects of NG stem from its ability to regulate genes involved in mitochondrial autophagy (<i>pink-1</i>, <i>pdr-1</i>); dopamine synthesis (<i>cat-2</i>); redox (<i>sod-3</i>) and protein folding homeostasis (<i>hsf-1</i>, <i>hsp-12.3</i>).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Neurogrit Gold has robust neuroprotective effects, making it a suitable treatment option against etiologies of Parkinson's disease.</p>\u0000 </section>\u0000 </div>","PeriodicalId":154,"journal":{"name":"CNS Neuroscience & Therapeutics","volume":"31 5","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cns.70401","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SorCS2 Is Important for Astrocytic Function in Neurovascular Signaling","authors":"Christian Staehr,&nbsp;Hande Login,&nbsp;Elizaveta V. Melnikova,&nbsp;Magdalena Bakun,&nbsp;Ewelina Ziemlinska,&nbsp;Lilian Kisiswa,&nbsp;Simin Berenji Ardestani,&nbsp;Stella Solveig Nolte,&nbsp;Hans Christian Beck,&nbsp;Line Mathilde Brostrup Hansen,&nbsp;Dmitry Postnov,&nbsp;Alexei Verkhratsky,&nbsp;Anna R. Malik,&nbsp;Anders Nykjaer,&nbsp;Vladimir V. Matchkov","doi":"10.1111/apha.70052","DOIUrl":"https://doi.org/10.1111/apha.70052","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>The receptor SorCS2 is involved in the trafficking of membrane receptors and transporters. It has been implicated in brain disorders and has previously been reported to be indispensable for ionotropic glutamatergic neurotransmission in the hippocampus.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>We aimed to study the role of SorCS2 in the control of astrocyte-neuron communication, critical for neurovascular coupling.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Brain slices from P8 and 2-month-old wild-type and SorCS2 knockout (<i>Sorcs2</i>^{<i>−/−</i>}) mice were immunostained for SorCS2, GFAP, AQP4, IB4, and CD31. Neurovascular coupling was assessed in vivo using laser speckle contrast imaging and ex vivo in live brain slices loaded with calcium-sensitive dye. Bulk and cell surface fraction proteomics was analyzed on freshly isolated and cultured astrocytes, respectively, and validated with Western blot and qPCR.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>SorCS2 was strongly expressed in astrocytes, primarily in their endfeet, of P8 mice; however, it was sparsely represented in 2-month-old mice. <i>Sorcs2</i>^{<i>−/−</i>} mice demonstrated reduced neurovascular coupling associated with a reduced astrocytic calcium response to neuronal excitation. No differences in vascularization or endothelium-dependent relaxation ex vivo between the 2-month-old groups were observed. Proteomics suggested changes in glutamatergic signaling and suppressed calcium signaling in <i>Sorcs2</i>^{<i>−/−</i>} brains from both P8 and 2-month-old mice. The increased abundance of glutamate metabotropic receptor 3 in <i>Sorcs2</i>^{<i>−/−</i>} astrocytes was validated by PCR and Western blot. In cultured <i>Sorcs2</i>^{<i>−/−</i>} astrocytes, AQP4 abundance was increased in the bulk lysate but reduced in the cell surface fraction, suggesting impaired trafficking.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The results suggest that SorCS2 expression is important for the development of neurovascular coupling, at least in part by modulating glutamatergic and calcium signaling in astrocytes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":107,"journal":{"name":"Acta Physiologica","volume":"241 6","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apha.70052","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front Cover: Engineering MoFe Nanostructures on Carbon Cloth for Sustainable Ammonia Production via Nitrogen Reduction (ChemCatChem 9/2025)","authors":"Shinyoung Kweon,&nbsp;Nilam Qureshi,&nbsp;Jun Ho Shim","doi":"10.1002/cctc.202580901","DOIUrl":"https://doi.org/10.1002/cctc.202580901","url":null,"abstract":"<p><b>The Front Cover</b> illustrates a MoFe/CC catalyst grown directly on carbon cloth and functioning as an efficient electrode for electrochemical ammonia synthesis. The optimized MoFe(7:3)/CC electrode enhances NH3 selectivity by suppressing the hydrogen evolution reaction at neutral pH. This catalyst achieves a high NH₃ production rate and Faradaic efficiency, thus demonstrating its potential for sustainable ammonia synthesis. More information can be found in the Research Article by S. Kweon, N. Qureshi and J. H. Shim (DOI: 10.1002/cctc.202401936).\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":141,"journal":{"name":"ChemCatChem","volume":"17 9","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cctc.202580901","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lane-Changing-Enabled Eco Approach Control for Autonomous Vehicles at a Signalized Intersection in Mixed Traffic Environment","authors":"Jiaqi Liu,&nbsp;Ting Qu,&nbsp;Shiying Dong,&nbsp;Bingzhao Gao","doi":"10.1049/itr2.70036","DOIUrl":"https://doi.org/10.1049/itr2.70036","url":null,"abstract":"<p>In complex urban traffic environments, vehicle-to-everything technology can reduce energy consumption and optimize travel time for vehicles at a signalized intersection. We propose a lane-changing-enabled eco approach control strategy that considers constraints from surrounding vehicles, traffic lights, and queues ahead. Our strategy adopts a hierarchical receding horizon control framework that provides the connected and autonomous vehicle with optimal lane and speed planning for adapting to dynamic traffic environment. In the upper level, we set up virtual traffic light for each lane via a mixed vehicle platoon model, utilizing their signal phase time constraints and constraints of surrounding vehicles to formulate a mixed-integer nonlinear programming problem to obtain optimal lane and speed reference. In the lower level, a car-following model is conducted in time domain and the reference speed is put into the controller to obtain the optimal velocity. Numerical experiment results show that our strategy is superior to regular eco-approach and departure strategy and lane-changing-enabled car-following strategy in terms of energy consumption reduction in the traffic scenarios without lane changing. In the traffic scenarios with lane changing, our strategy improves traffic efficiency by reducing travel time.</p>","PeriodicalId":50381,"journal":{"name":"IET Intelligent Transport Systems","volume":"19 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/itr2.70036","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Docosahexaenoic Acid and Nervonic Acid Synergically Enhance Cognitive Memory in Normal Mice via Brain Fatty Acids Remodeling","authors":"Hai-Yan Ou,&nbsp;Lei Feng,&nbsp;Rui-Ye Chen,&nbsp;Hui-Yun Lu,&nbsp;Shuang Cai,&nbsp;Zhi-You Yang","doi":"10.1002/efd2.70059","DOIUrl":"https://doi.org/10.1002/efd2.70059","url":null,"abstract":"<p>Docosahexaenoic acid (DHA) and nervonic acid (NA) are the main components of brain gray and white matter, respectively. Preclinical and clinical studies suggest the effects of DHA or NA supplementation on memory improvement in aging and Alzheimer's disease models. However, it is not yet clear whether dietary supplementation with DHA and NA can enhance memory by increasing their levels in gray and white matter. The present study aimed to examine the spatial memory and brain white and gray matter lipidomic profiles in adolescent mice with or without a DHA and NA-enriched diet. Spatial memory and conditional fear memory were evaluated in adolescent mice using the Morris Water Maze and passive avoidance test beginning at 7-week of age and mice were killed at 13-week of age to allow for the dissection of brain tissues. Combined low dose treatment of DHA (600 mg/kg) and NA (94 mg/kg) synergically enhanced spatial memory and fear memory function, and the effects were better than that of DHA intervention. While, high dose of DHA and NA treatment was detrimental to long-term spatial memory and fear memory, corresponding to the decreased levels of serotonin (5-HT) in the cortex and hippocampus, and imbalanced fatty acids in the white and gray matter. In addition, c-Fos positive neurons were increased in low dose DHA treated mice comparing to that of control mice. Lipidomic analyses suggest levels of NA, dihomo-γ-linolenic acid, EPA, DHA, and linoleic acid were increased in the white matter post low dose of DHA + NA treatment, as well as increased levels of dihomo-γ-linolenic acid, EPA, and DHA in the gray matter compared with control mice. Understanding how DHA and NA supplementation during the adolescent periods affects cognitive function in a dose-specific manner has important implications for determining the dietary requirements of DHA and NA. The present study reported for the first time that DHA and NA combination regulate spatial memory in a dose-specific manner and provides evidence that further research needs to consider how different ratios of DHA and NA regulate the fatty acid composition in white and gray matter, thereby influencing memory function.</p>","PeriodicalId":11436,"journal":{"name":"eFood","volume":"6 3","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70059","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Isolated SEPIC-Based AC-AC Converter for Voltage Sag/Swell Mitigation","authors":"Iman Abdoli,&nbsp;Alireza Lahooti Eshkevari,&nbsp;Hafiz Furqan Ahmed,&nbsp;Mohammad Arasteh","doi":"10.1049/pel2.70044","DOIUrl":"https://doi.org/10.1049/pel2.70044","url":null,"abstract":"<p>This paper proposes a high-frequency isolated buck-boost AC-AC converter based on the single-ended-primary-inductance-converter (SEPIC) topology and its extension circuit with bipolar output. The proposed converters can achieve a wide range of voltage step-down and step-up operations with both same- and reversed-phase outputs. All configurations ensure low harmonic input current and are fully compatible with non-unity power factor loads. Comparisons with counterpart converters demonstrate that this topology outperforms in terms of inductor volume metrics, total switch voltage, and power switch utilisation factor. Unlike most counterparts, only one of the four power switches in the proposed topology operates at a high frequency in each voltage half-cycle, minimising switching losses and enhancing efficiency. Additionally, snubber circuits are eliminated, ensuring spike-free operation for power switches. The proposed bipolar output converter can be easily connected in series with the load as a dynamic voltage restorer without a line-frequency transformer. The topology benefits from continuous input current and regular operation with inductive loads. In this article, theoretical analysis is presented and supported by laboratory hardware verification. A 250W prototype has been built and tested as a dynamic voltage restorer (DVR). Results confirm the operation of the converter and demonstrate its capability to mitigate deep voltage sags/swells (≥ 50%) due to high-gain operation.</p>","PeriodicalId":56302,"journal":{"name":"IET Power Electronics","volume":"18 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/pel2.70044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing Sustainability in Fruit and Vegetable Packaging: The Role of Nanotechnology in Food Preservation","authors":"Farhang Hameed Awlqadr,&nbsp;Ammar B. Altemimi,&nbsp;Ali Muhi Aldeen Omar,&nbsp;Mohammed N. Saeed,&nbsp;Syamand Ahmed Qadir,&nbsp;Aryan Mahmood Faraj,&nbsp;Alaa Jabbar Abd Al-Manhel,&nbsp;Tablo H. Salih,&nbsp;Mohammad Ali Hesarinejad,&nbsp;Italo Rennan Sousa Vieira","doi":"10.1002/efd2.70060","DOIUrl":"https://doi.org/10.1002/efd2.70060","url":null,"abstract":"<p>Food packaging has seen a technological revolution through nanotechnology that provides highly innovative solutions to the increase of preservation, quality, and shelf life of fruits and vegetables. Moreover, this review has an in-depth examination of the most advanced nanotechnology packaging that realizes a significant increase in barrier properties, it is involved in including antimicrobial method, and is a pioneer in making use of intelligent packaging systems. Different nanomaterials, such as nano-coatings, nano-films, and nanofibers have proven their effectiveness in modulating oxygen and moisture permeability and repressing microbial contamination along with systems for real-time environmental monitoring. Furthermore, the incorporation of biodegradable nanocomposite films into the market provides a bio-based alternative to conventional packaging, which is a cheaper and environmentally friendly way of maintaining food safety and freshness. However, despite such developments, it can be seen that there are a number of challenges that still require a bit further probing. The future research should concern the most comprehensive analysis of nanoparticles that migrate from the packaging into the products and how they may affect people in the case of long-term exposure. Moreover, incorporating regulations related to the usage of nanotechnologies should be standardized to ensure that the adoption of nanotechnology in the food packaging process is safe and responsible. Green technology made the innovations in bio-based nanomaterials and eco-friendly nano-coatings become a reality, which further promoted the sustainability of the gains and still brought all the benefits intact. Additionally, integrating smart packaging systems with nanosensors will offer the possibility of food waste reduction and improvement of supply chain efficiency through real-time food quality monitoring. Continued interdisciplinary collaboration between food scientists, material engineers, and regulatory authorities will be crucial to addressing these challenges and unlocking the full potential of nanotechnology in food packaging.</p>","PeriodicalId":11436,"journal":{"name":"eFood","volume":"6 3","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significance of Mutation Spots and Concurrent Gene Mutations on Prognosis and Clinical Outcomes in Myelodysplastic Syndromes With SF3B1 Mutation","authors":"Qi Liu,&nbsp;Fanhuan Xu,&nbsp;Juan Guo,&nbsp;Feng Xu,&nbsp;Xinhui Huang,&nbsp;Jianan Chen,&nbsp;Jiacheng Jin,&nbsp;Liyu Zhou,&nbsp;Qi He,&nbsp;Dong Wu,&nbsp;Luxi Song,&nbsp;Zheng Zhang,&nbsp;Cha Guo,&nbsp;Jiying Su,&nbsp;Yumei Zhang,&nbsp;Meng Yan,&nbsp;Chunkang Chang,&nbsp;Xiao Li,&nbsp;Lingyun Wu","doi":"10.1002/cam4.70930","DOIUrl":"https://doi.org/10.1002/cam4.70930","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Purpose</h3>\u0000 \u0000 <p>To investigate the clinical characteristics and prognosis of mutation spots and concomitant gene mutations in myelodysplastic syndromes (MDS) with <i>SF3B1</i> mutation (<i>SF3B1</i>^<i>mut</i>).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Patients and Methods</h3>\u0000 \u0000 <p>Patients diagnosed with MDS at Shanghai Jiao Tong University School of Medicine Affiliated Sixth People's Hospital from October 2008 to November 2023 were enrolled in this study. <i>SF3B1</i>^<i>mut</i> was identified by next-generation sequencing (NGS).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>One hundred and seven (8.7%) cases harbored the <i>SF3B1</i> mutation. The most frequent <i>SF3B1</i>^<i>mut</i>, noted in 47.66% of all patients, was the hotspot <i>K700E</i>. <i>K666</i> and <i>R625</i> were observed in 24.30% and 9.35%, respectively. Two less frequent mutation subtypes accounted for 5.61% of <i>H662</i> and 4.67% of <i>E622</i>. Patients with the <i>K666</i> mutation showed more severe thrombocytopenia (<i>p</i> = 0.032), significantly lower NK cell percentage (<i>p</i> = 0.001), and the Th1/Th2 ratio (<i>p</i> = 0.018) in the bone marrow (BM). The overall survival (OS) in patients with <i>E622</i> and <i>H662</i> mutations was significantly longer than that of patients with the <i>R625</i> mutation (<i>p</i> = 0.045) and the <i>K666</i> mutation (<i>p</i> = 0.010). Multi-variance analysis showed the <i>SF3B1</i> mutation involving the <i>K666</i> hotspot independently predicted overall survival in MDS (HR 2.094, <i>p</i> = 0.050). Notably, most (11/13, 84.6%) of concomitant <i>TP53</i> mutations were mono-hit, which did not affect the survival of patients in our cohort.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p><i>SF3B1</i>^<i>mut</i> patients with specific mutation spots and concomitant gene mutations showed distinct clinical features and prognosis. Consequently, a comprehensive study of specific subtypes is of great significance for improving the prognosis of patients with <i>SF3B1</i> mutations.</p>\u0000 </section>\u0000 </div>","PeriodicalId":139,"journal":{"name":"Cancer Medicine","volume":"14 9","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cam4.70930","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant PRV Expressing GP3 and GP5 of PRRSV Provides Effective Protection Against Coinfection With PRV and PRRSV","authors":"Ruhai Guo,&nbsp;Hui Li,&nbsp;Junda Li,&nbsp;Jahao Qv,&nbsp;Guofan Ren,&nbsp;Xiao Zhang,&nbsp;Saba Nasir,&nbsp;Jingnan Zhang,&nbsp;Chen Luo,&nbsp;Basit Zeshan,&nbsp;Yefei Zhou,&nbsp;Honglin Xie,&nbsp;Xinglong Wang","doi":"10.1155/tbed/4612568","DOIUrl":"https://doi.org/10.1155/tbed/4612568","url":null,"abstract":"<div>\u0000 <p>Porcine Reproductive and Respiratory Syndrome (PRRS) and Pseudorabies (PR) are highly contagious diseases caused by Porcine Reproductive and Respiratory Syndrome virus (PRRSV) and Pseudorabies virus (PRV). Due to the limited protective ability of currently commercialized vaccines against NADC30-like PRRSV and PRV variants, the pathological damage caused by coinfection of these two viruses has a significant impact on China’s pig farming industry. In this study, six recombinant PRV stains with TK and gI/gE deletions and fused expression of GM-CSF and GP3 and GP5 proteins from NADC30-Like PRRSV were constructed by using the HDR-CRISPR/Cas9^D10A system. After assessing growth characteristics and genetic stability, four strains demonstrating stable proliferation and expression of the GM-CSF, GP3, GP5 fusion protein in BHK-21 cells were selected. Evaluation of their ability to induce specific humoral and cellular immune responses in mice led to the selection of two strains with superior immunogenic effects: rPRV-ΔTK-GP3-GP5-eGFP-ΔgI/gE-mCHERRY-B and rPRV-ΔTK-eGFP-ΔgI/gE-GP3-GP5-mCHERRY-B. These strains were found to enhance NADC30-like PRRSV and PRV-specific immune responses in piglets, reduce pathological damage, and accelerate symptom resolution. In general, PRV is a promising viral vector for expressing PRRSV genes, and the data from this study provides references for new candidate vaccines against PRRSV.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/4612568","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}