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The Neurobehavioral Symptom Inventory: Psychometric Properties and Symptom Comparisons in Women With and Without Brain Injuries Due to Intimate Partner Violence. 神经行为症状量表:亲密伴侣暴力导致脑损伤和未造成脑损伤的女性的心理计量特性和症状比较》(Psychometric Properties and Symptom Comparisons in Women With and Without Brain Jury due to Intimate Partner Violence.
IF 4.3 2区 心理学
Assessment Pub Date : 2025-01-01 Epub Date: 2024-03-14 DOI: 10.1177/10731911241236687
Justin E Karr, Agnes E White, Sharon E Leong, T K Logan
{"title":"The Neurobehavioral Symptom Inventory: Psychometric Properties and Symptom Comparisons in Women With and Without Brain Injuries Due to Intimate Partner Violence.","authors":"Justin E Karr, Agnes E White, Sharon E Leong, T K Logan","doi":"10.1177/10731911241236687","DOIUrl":"10.1177/10731911241236687","url":null,"abstract":"<p><p>This study psychometrically evaluated the Neurobehavioral Symptom Inventory (NSI) among women survivors of intimate partner violence (IPV) and compared symptoms between women with no brain injury history (<i>n</i> = 93) and women with IPV-related brain injury history (<i>n</i> = 112). Women completed the NSI and questionnaires on traumatic brain injury (TBI), hypoxic-ischemic brain injury (HI-BI), and lifetime IPV history. A four-factor NSI model, including affective, somatosensory, cognitive, and vestibular factors, had the best fit (comparative fit index = 0.970, root mean square error of approximation = 0.064), with strong reliability for the total score (<i>ω</i> = .93) and subscale scores (<i>ω</i> range = .72-.89). In group comparisons, women with IPV-related brain injuries reported greater total, affective, and cognitive symptom severity after adjusting for age and education; however, no group differences were observed after adjusting for IPV severity. When examining lifetime number of brain injuries, HI-BI count was independently predictive of total, cognitive, and vestibular symptom severity after adjusting for age, education, and IPV severity; whereas TBI count did not independently predict any NSI scores after adjusting for these covariates. The NSI had acceptable psychometric properties for measuring neurobehavioral symptoms among women survivors of IPV. The association between HI-BI count and cognitive and vestibular symptoms may indicate the importance of studying repetitive nonfatal strangulation as an injury mechanism in this population.</p>","PeriodicalId":8577,"journal":{"name":"Assessment","volume":" ","pages":"102-118"},"PeriodicalIF":4.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140118677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Measuring Process Factors of Fluid Reasoning Using Multidimensional Computerized Adaptive Testing. 利用多维计算机适应性测试测量流体推理的过程因素。
IF 4.3 2区 心理学
Assessment Pub Date : 2025-01-01 Epub Date: 2024-03-16 DOI: 10.1177/10731911241236351
Hanif Akhtar, Kristof Kovacs
{"title":"Measuring Process Factors of Fluid Reasoning Using Multidimensional Computerized Adaptive Testing.","authors":"Hanif Akhtar, Kristof Kovacs","doi":"10.1177/10731911241236351","DOIUrl":"10.1177/10731911241236351","url":null,"abstract":"<p><p>Although many fluid reasoning (Gf) tests have been developed, there is a lack of figural tests measuring its lower-order process factors simultaneously. The present article introduces the development of the Multidimensional Induction-Deduction Computerized Adaptive Test (MID-CAT) to measure two process factors of Gf. The MID-CAT is designed to provide an instrument that is flexible, efficient, and entirely free for non-commercial use. We created 530 items and administered them to a sample of <i>N</i> = 2,247. Items were fitted and calibrated using the Rasch model. The results indicate that the final item pool has a wide range of difficulties that could precisely measure a wide range of test-takers' abilities. A simulation study also indicates that MID-CAT provides greater measurement efficiency than separate-unidimensional CAT or fixed-item test. In the discussion, we provide perspectives on how the MID-CAT can be used for future research.</p>","PeriodicalId":8577,"journal":{"name":"Assessment","volume":" ","pages":"32-47"},"PeriodicalIF":4.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140139840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Age-Neutral Measurement Of Personality Functioning and Maladaptive Personality Traits. 人格功能和不良人格特质的年龄中性测量。
IF 4.3 2区 心理学
Assessment Pub Date : 2025-01-01 Epub Date: 2024-03-28 DOI: 10.1177/10731911241240626
M F Facon, S P J van Alphen, E Dierckx, G Rossi
{"title":"Age-Neutral Measurement Of Personality Functioning and Maladaptive Personality Traits.","authors":"M F Facon, S P J van Alphen, E Dierckx, G Rossi","doi":"10.1177/10731911241240626","DOIUrl":"10.1177/10731911241240626","url":null,"abstract":"<p><p>As previous studies have shown that personality disorder (PD) assessment in older adults is often hampered because assessment tools are tailored toward younger adults, establishing the age-neutrality of novel tools is crucial. This study primarily aimed to evaluate the age-neutrality of the Level of Personality Functioning Brief Form (LPFS-BF 2.0) and the Personality Inventory for <i>DSM</i>-5 Modified + (PID-5-BF+M), using a sample of 254 community-dwelling adults. The analysis of Differential Item Functioning (DIF) demonstrated the age-neutrality of both instruments, with only 8.3% of LPFS-BF 2.0 items and 5.6% of PID-5-BF+M items exhibiting DIF. Differential Test Functioning (DTF) analyses revealed large DTF for the LPFS-BF 2.0 total score, indicating that age-specific norms might be necessary for this score. In summary, this study supports the use of these instruments in both older and younger adults, enhancing the assessment of PDs across the life span.</p>","PeriodicalId":8577,"journal":{"name":"Assessment","volume":" ","pages":"3-13"},"PeriodicalIF":4.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140317699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Macrophage‑driven pathogenesis in acute lung injury/acute respiratory disease syndrome: Harnessing natural products for therapeutic interventions (Review). 急性肺损伤/急性呼吸道疾病综合征中巨噬细胞驱动的发病机制:利用天然产品进行治疗干预(综述)。
IF 4.3 3区 医学
Molecular medicine reports Pub Date : 2025-01-01 Epub Date: 2024-11-08 DOI: 10.3892/mmr.2024.13381
Jincun Li, Wenyu Ma, Zilei Tang, Yingming Li, Ruiyu Zheng, Yuhuan Xie, Gang Li
{"title":"Macrophage‑driven pathogenesis in acute lung injury/acute respiratory disease syndrome: Harnessing natural products for therapeutic interventions (Review).","authors":"Jincun Li, Wenyu Ma, Zilei Tang, Yingming Li, Ruiyu Zheng, Yuhuan Xie, Gang Li","doi":"10.3892/mmr.2024.13381","DOIUrl":"10.3892/mmr.2024.13381","url":null,"abstract":"<p><p>Acute lung injury (ALI) or acute respiratory distress syndrome (ARDS) is a common respiratory disease characterized by hypoxemia and respiratory distress. It is associated with high morbidity and mortality. Due to the complex pathogenesis of ALI, the clinical management of patients with ALI/ARDS is challenging, resulting in numerous post‑treatment sequelae and compromising the quality of life of patients. Macrophages, as a class of innate immune cells, play an important role in ALI/ARDS. In recent years, the functions and phenotypes of macrophages have been better understood due to the development of flow cytometry, immunofluorescence, single‑cell sequencing and spatial genomics. However, no macrophage‑targeted drugs for the treatment of ALI/ARDS currently exist in clinical practice. Natural products are important for drug development, and it has been shown that numerous natural compounds from herbal medicine can alleviate ALI/ARDS caused by various factors by modulating macrophage abnormalities. In the present review, the natural products from herbal medicine that can modulate macrophage abnormalities in ALI/ARDS to treat ALI/ARDS are introduced, and their mechanisms of action, discovered in the previous five years (2019‑2024), are presented. This will provide novel ideas and directions for further research, to develop new drugs for the treatment of ALI/ARDS.</p>","PeriodicalId":18818,"journal":{"name":"Molecular medicine reports","volume":"31 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551695/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Melittin promotes the proliferation of Schwann cells in hyperglycemic environment by up‑regulating the Crabp2/Wnt/β‑catenin signaling pathway. Melittin 通过上调 Crabp2/Wnt/β-catenin 信号通路,促进高血糖环境下许旺细胞的增殖。
IF 4.3 3区 医学
Molecular medicine reports Pub Date : 2025-01-01 Epub Date: 2024-10-25 DOI: 10.3892/mmr.2024.13371
Qiuyi Zhang, Yuxia Chen, Wei Huang, Jiaqian Zhou, Dawei Yang
{"title":"Melittin promotes the proliferation of Schwann cells in hyperglycemic environment by up‑regulating the Crabp2/Wnt/β‑catenin signaling pathway.","authors":"Qiuyi Zhang, Yuxia Chen, Wei Huang, Jiaqian Zhou, Dawei Yang","doi":"10.3892/mmr.2024.13371","DOIUrl":"10.3892/mmr.2024.13371","url":null,"abstract":"<p><p>The present study aimed to explore the effect of melittin (MLT) on the growth of Schwann cells (SCs) in high glucose conditions and to understand the mechanisms involved. The goal was to provide a theoretical basis for using MLT in the treatment of diabetic peripheral neuropathy (DPN). The CCK‑8 assay was used to measure cell activity at different concentrations of glucose and MLT. Flow cytometry was employed to analyze the effect of MLT on cell cycle phases and apoptosis in SCs under high glucose conditions. To identify differentially expressed proteins, 4D label‑free quantitative proteomics with liquid chromatography‑mass spectrometry was used, followed by biological analysis to explore potential mechanisms. PCR, western blotting and immunofluorescence were conducted to confirm these mechanisms. Melittin (0.2 <i>µ</i>g/ml) increased the proliferation of SCs in a high glucose environment. Flow cytometry showed that after MLT treatment, the proportion of cells in the G<sub>2</sub>/M+S phase increased and the combined ratio of early and late apoptosis decreased under high glucose conditions. Proteomics identified 1,784 proteins with significant changes in expression; 725 were upregulated, and 1,059 were downregulated. Kyoto Encyclopedia of Genes and Genomes analysis indicated that the differentially expressed proteins were mainly involved in metabolic pathways and neurodegenerative disease pathways. PCR, western blotting and immunofluorescence confirmed the increase in Crabp2, Wnt3a, C‑Jun, CDK4, CyclinD1 and proliferating cell nuclear antigen. In high glucose conditions, MLT protects SCs from glucose toxicity by upregulating the Crabp2/Wnt/β‑catenin signaling pathway, potentially providing a new treatment for DPN.</p>","PeriodicalId":18818,"journal":{"name":"Molecular medicine reports","volume":"31 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessing Between- and Within-Person Reliabilities of Items and Scale for Daily Procrastination: A Multilevel and Dynamic Approach. 评估日常拖延症的项目和量表在人与人之间和人与人之间的可靠性:多层次动态方法。
IF 4.3 2区 心理学
Assessment Pub Date : 2025-01-01 Epub Date: 2024-03-18 DOI: 10.1177/10731911241235467
Xiaohui Luo, Yueqin Hu, Hongyun Liu
{"title":"Assessing Between- and Within-Person Reliabilities of Items and Scale for Daily Procrastination: A Multilevel and Dynamic Approach.","authors":"Xiaohui Luo, Yueqin Hu, Hongyun Liu","doi":"10.1177/10731911241235467","DOIUrl":"10.1177/10731911241235467","url":null,"abstract":"<p><p>Intensive longitudinal data (ILD) has been collected to capture the dynamic fluctuations of procrastination; however, researchers have typically measured daily procrastination by modifying trait measures (e.g., adding a time reference \"today\") without adequately testing their reliabilities. The main purpose of this study was to use an advanced approach, dynamic structural equation modeling, to assess the between- and within-person reliabilities of a widely used six-item measure of daily procrastination. A total of 252 participants completed retrospective measures of various types of trait procrastination and daily measures of procrastination over 34 consecutive days. The results showed that the entire scale for daily procrastination and five of its six items had high between- and within-person reliabilities, but one item had much lower reliabilities, suggesting that this item may be inappropriate in everyday contexts. Furthermore, we found moderate to strong associations between the latent trait factor of procrastination and trait measures of procrastination. In addition, we identified substantial between-person variation in person-specific reliabilities and explored its relevant factors. Overall, this study assessed the reliabilities of a daily measure of procrastination, which facilitated future studies to obtain more reliable and consistent results and to better estimate the reliability of ILD.</p>","PeriodicalId":8577,"journal":{"name":"Assessment","volume":" ","pages":"61-76"},"PeriodicalIF":4.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140142655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Selective protein degradation through chaperone‑mediated autophagy: Implications for cellular homeostasis and disease (Review). 通过伴侣介导的自噬选择性降解蛋白质:对细胞稳态和疾病的影响(综述)。
IF 4.3 3区 医学
Molecular medicine reports Pub Date : 2025-01-01 Epub Date: 2024-11-08 DOI: 10.3892/mmr.2024.13378
Jiahui Huang, Jiazhen Wang
{"title":"Selective protein degradation through chaperone‑mediated autophagy: Implications for cellular homeostasis and disease (Review).","authors":"Jiahui Huang, Jiazhen Wang","doi":"10.3892/mmr.2024.13378","DOIUrl":"10.3892/mmr.2024.13378","url":null,"abstract":"<p><p>Cells rely on autophagy for the degradation and recycling of damaged proteins and organelles. Chaperone-mediated autophagy (CMA) is a selective process targeting proteins for degradation through the coordinated function of molecular chaperones and the lysosome‑associated membrane protein‑2A receptor (LAMP2A), pivotal in various cellular processes from signal transduction to the modulation of cellular responses under stress. In the present review, the intricate regulatory mechanisms of CMA were elucidated through multiple signaling pathways such as retinoic acid receptor (RAR)α, AMP‑activated protein kinase (AMPK), p38‑TEEB‑NLRP3, calcium signaling‑NFAT and PI3K/AKT, thereby expanding the current understanding of CMA regulation. A comprehensive exploration of CMA's versatile roles in cellular physiology were further provided, including its involvement in maintaining protein homeostasis, regulating ferroptosis, modulating metabolic diversity and influencing cell cycle and proliferation. Additionally, the impact of CMA on disease progression and therapeutic outcomes were highlighted, encompassing neurodegenerative disorders, cancer and various organ‑specific diseases. Therapeutic strategies targeting CMA, such as drug development and gene therapy were also proposed, providing valuable directions for future clinical research. By integrating recent research findings, the present review aimed to enhance the current understanding of cellular homeostasis processes and emphasize the potential of targeting CMA in therapeutic strategies for diseases marked by CMA dysfunction.</p>","PeriodicalId":18818,"journal":{"name":"Molecular medicine reports","volume":"31 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11542157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Low Overlap and High Heterogeneity Across Common Measures of Eating Disorder Pathology: A Content Analysis. 饮食失调症病理的通用测量方法存在低重叠和高异质性:内容分析
IF 4.3 2区 心理学
Assessment Pub Date : 2025-01-01 Epub Date: 2024-03-22 DOI: 10.1177/10731911241238084
Kara A Christensen Pacella, Lidia Wossen, Kelsey E Hagan
{"title":"Low Overlap and High Heterogeneity Across Common Measures of Eating Disorder Pathology: A Content Analysis.","authors":"Kara A Christensen Pacella, Lidia Wossen, Kelsey E Hagan","doi":"10.1177/10731911241238084","DOIUrl":"10.1177/10731911241238084","url":null,"abstract":"<p><p>This study evaluated symptoms assessed in common measures of eating disorder pathology and tested overlap to evaluate the extent to which measures may be interchangeable. Six measures were included: Bulimia Test-Revised, Eating Attitudes Test-26, Eating Disorder Diagnostic Scale, Eating Disorder Examination Questionnaire, Eating Pathology Symptoms Inventory, and Questionnaire for Eating Disorder Diagnoses. Content overlap was quantitatively estimated using the Jaccard Index. Mean overlap was low (.195), likely due to the wide range of symptoms (87) assessed. The mean overlap of each measure with all others was .117 - .267, and the overlap among individual measures was .083 - .382. Implications of low overlap among measures include variable characterization of eating disorder phenotypes and the risk for lower generalizability of findings due to measurement variability.</p>","PeriodicalId":8577,"journal":{"name":"Assessment","volume":" ","pages":"48-60"},"PeriodicalIF":4.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140193171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Corrigendum] Tripartite motif‑containing 11 regulates the proliferation and apoptosis of breast cancer cells. [更正] 含三方图案的 11 调节乳腺癌细胞的增殖和凋亡。
IF 4.3 3区 医学
Oncology reports Pub Date : 2025-01-01 Epub Date: 2024-11-08 DOI: 10.3892/or.2024.8838
Xianping Dai, Feng Geng, Mengshun Li, Ming Liu
{"title":"[Corrigendum] Tripartite motif‑containing 11 regulates the proliferation and apoptosis of breast cancer cells.","authors":"Xianping Dai, Feng Geng, Mengshun Li, Ming Liu","doi":"10.3892/or.2024.8838","DOIUrl":"10.3892/or.2024.8838","url":null,"abstract":"<p><p>Subsequently to the publication of the above paper, an interested reader drew to the authors' attention that, concerning the flow cytometric plots shown in Fig. 5A and B on p. 2572, each figure part contained a pair of duplicated data panels; specifically, the panels depicting the 'NC/5‑FU' and the 'shTRIM11/Gemcitabine' experiments in Fig 5A (MCF‑7 cells), and the 'NC/Paclitaxel' and 'shTRIM11/Adriamycin' experi-ments in Fig. 5B (MDA‑MB‑231 cells), were apparently identical. The authors were able to re‑examine their original data files, and realize that this figure was inadverently assembled incorrectly. The revised version of Fig. 5, now showing the correct data for the 'shTRIM11/Gemcitabine' experiment in Fig 5A and the 'NC/Paclitaxel' experiment in Fig. 5B, is shown on the next page. Note that the revisions made to this figure do not affect the overall conclusions reported in the paper. The authors are grateful to the Editor of <i>Oncology Reports</i> for allowing them the opportunity to publish this Corrigendum, and apologize to the readership for any inconvenience caused. [Oncology Reports 41: 2567‑2574, 2019; DOI: 10.3892/or.2019.7015].</p>","PeriodicalId":19527,"journal":{"name":"Oncology reports","volume":"53 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11566101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142605765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Long non‑coding RNA SNHG1 promotes autophagy in vascular smooth muscle cells induced by facilitating CLEC7A. 长非编码 RNA SNHG1 在促进 CLEC7A 的诱导下促进血管平滑肌细胞的自噬。
IF 4.3 3区 医学
Molecular medicine reports Pub Date : 2025-01-01 Epub Date: 2024-11-08 DOI: 10.3892/mmr.2024.13385
Hao-Wei Deng, Wen-Bin Teng, Shao-Dan Zhou, Zi-Ming Ye, Zi-Mei Dong, Rui-Ting Hu, Chao Qin
{"title":"Long non‑coding RNA SNHG1 promotes autophagy in vascular smooth muscle cells induced by facilitating CLEC7A.","authors":"Hao-Wei Deng, Wen-Bin Teng, Shao-Dan Zhou, Zi-Ming Ye, Zi-Mei Dong, Rui-Ting Hu, Chao Qin","doi":"10.3892/mmr.2024.13385","DOIUrl":"10.3892/mmr.2024.13385","url":null,"abstract":"<p><p>Long non‑coding RNAs serve a crucial role in autophagy of vascular smooth muscle cells (VSMCs). The present study aimed to investigate the effect of small nucleolar RNA host gene 1 (SNHG1) on autophagy in VSMCs and the associated underlying mechanisms. Rapamycin was used to induce autophagy in VSMCs and the effects of SNHG1 on the proliferation and migration of VSMCs and the change in phenotype were tested following overexpression and silencing of SNHG1. The target gene of SNHG1 was predicted and validated. SNHG1‑regulated autophagy of VSMCs via C‑type lectin domain family 7 member A (CLEC7A) was determined by combined silencing of SNHG1 and overexpression of CLEC7A. Rapamycin‑induced autophagy in VSMCs changed the cell phenotype from contractile to synthetic, with decreased expression of α‑smooth muscle actin and smooth muscle protein 22a and increased expression of osteopontin. Overexpression of SNHG1 caused the same change in phenotype while the opposite change was observed following SNHG1 silencing. Overexpression of SNHG1 promoted the proliferation and migration of VSMCs. CLEC7A was identified as a target gene of SNHG1 and a direct binding relationship between them was confirmed by RNA immunoprecipitation and RNA pull‑down assays. Overexpression of SNHG1 increased the expression of CLEC7A. The expression of both SNHG1 and CLEC7A was increased during autophagy of VSMCs. Overexpression of SNHG1 promoted autophagy of VSMCs and silencing of CLEC7A reduced this effect of SNHG1. In conclusion, SNHG1 and CLEC7A were increased in VSMCs following autophagy. SNHG1 promotes the conversion of VSMCs from a contractile phenotype to a synthetic phenotype by facilitating CLEC7A expression.</p>","PeriodicalId":18818,"journal":{"name":"Molecular medicine reports","volume":"31 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11564905/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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