Advanced Science最新文献

A Synergistic Complexation-Encapsulation Paradigm Enables Unprecedented Radioactive Remediation. 协同络合-封装模式使前所未有的放射性修复。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-10-03 DOI: 10.1002/advs.202504940

Qilong Tang, Huaixin Hao, Xue Dong, Chao Xu, Zhipeng Wang

引用次数: 0

Bioinspired Cellulose-Based Ultra-Slippery Film with Superior Transmittance, Anti-Fouling and De-Icing Properties for the Durable and Efficient Output of Solar Panels. 生物启发纤维素基超滑薄膜，具有优越的透光性，防污和除冰性能，用于太阳能电池板的耐用和高效输出。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-10-03 DOI: 10.1002/advs.202514626

Hujun Wang, Chuangqi Mo, Xueping Zhang, Jing Zheng, Gaohui Han, Haonan Qiu, Bo Li, Kai Yin, Zhongrong Zhou

{"title":"Bioinspired Cellulose-Based Ultra-Slippery Film with Superior Transmittance, Anti-Fouling and De-Icing Properties for the Durable and Efficient Output of Solar Panels.","authors":"Hujun Wang, Chuangqi Mo, Xueping Zhang, Jing Zheng, Gaohui Han, Haonan Qiu, Bo Li, Kai Yin, Zhongrong Zhou","doi":"10.1002/advs.202514626","DOIUrl":"https://doi.org/10.1002/advs.202514626","url":null,"abstract":"High optical transmittance can endow solar panels with sufficient light energy intake, while anti-fouling and anti-icing properties ensure stable power generation in environments where dust, bird droppings, algae, and ice are prone to accumulate. A highly transparent and ultra-slippery surface is promising for meeting these requirements. However, it remains a huge challenge to achieve superior transmittance, anti-fouling, anti-icing, and durability on the same surface to ensure high energy conversion efficiency for solar panels. Herein, a bioinspired cellulose-based ultra-slippery film (BCUSF) with an extremely low water sliding angle (SA = 0.4°) and high transmittance (≈95% of bake glass) is reported. Benefiting from the impressive slippery property, remarkably low ice adhesion strength (0.38 kPa), and superior self-cleaning and anti-fouling performances are also demonstrated. Moreover, the BCUSF exhibits excellent durability and robustness, maintaining a SA of 0.8° after suffering high shear at 9000 r min-1. Accordingly, the BCUSF with highly comprehensive performance enables solar panels to maintain high energy-conversion efficiency after repeated accumulation/cleaning of ice (ice adhesion strength = 0.91 kPa after 25 tests) and dust, or sand impact. It is envisioned that the BCUSF can boost the practical applications of slippery films on solar panels.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e14626"},"PeriodicalIF":14.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lignin-Containing Nanocellulose Mediated Interlayer Modulation Unlocks Stable and Redispersible MXene. 含木质素纳米纤维素介导的层间调制解锁稳定和可再分散的MXene。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-10-03 DOI: 10.1002/advs.202508665

Shuyang He, Zhen Yu, Shan Li, Shijie Lei, Lin Zhu, Ke Zhao, Fangxia Yang, Ningning Cao, Yuyan Liu, Zhimin Fan

{"title":"Lignin-Containing Nanocellulose Mediated Interlayer Modulation Unlocks Stable and Redispersible MXene.","authors":"Shuyang He, Zhen Yu, Shan Li, Shijie Lei, Lin Zhu, Ke Zhao, Fangxia Yang, Ningning Cao, Yuyan Liu, Zhimin Fan","doi":"10.1002/advs.202508665","DOIUrl":"https://doi.org/10.1002/advs.202508665","url":null,"abstract":"Titanium carbide (Ti3C2Tx) MXene combines exceptional conductivity, mechanical robustness, and multifunctionality, positioning it as a promising material for diverse applications. However, its industrial deployment remains hampered by the inability to precisely control redispersibility and oxidative stability. Herein, an interlayer chemical modulation strategy is reported, mediated by amphiphilic lignin-containing nanocellulose (LNC). Competitive interactions between the hydrophilic and hydrophobic segments of LNC within MXene interlayers enable precise tuning of spacing and interfacial chemistry. This approach allows rapid, industrial-scale spray drying to produce semi-solid MXene with long-term reversible redispersibility and outstanding oxidative stability. The resulting material can be fully redispersed into monolayer MXene even after 180 days of storage while maintaining high conductivity (≈7000 S cm-1). Moreover, by adjusting the post-drying time-dependent window, MXene powders can be programmably switched from a dynamically reversible to a permanently fixed structure, broadening their utility across multiple domains. This approach is expected to resolve the long-standing industry bottlenecks of MXene, including its susceptibility to oxidation, high transportation costs, and challenges in reprocessing, thereby opening a new path for the rapid transition of MXene from laboratory to commercial application.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e08665"},"PeriodicalIF":14.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sonoepigenetic Modification Mechanoprimes Early Osteogenic Commitment in Mesenchymal Stem Cells. 超声表观遗传修饰机制促进间充质干细胞早期成骨承诺。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-10-03 DOI: 10.1002/advs.202509860

Lizebona A Ambattu, Blanca Del Rosal, Carmelo Ferrai, Leslie Y Yeo

{"title":"Sonoepigenetic Modification Mechanoprimes Early Osteogenic Commitment in Mesenchymal Stem Cells.","authors":"Lizebona A Ambattu, Blanca Del Rosal, Carmelo Ferrai, Leslie Y Yeo","doi":"10.1002/advs.202509860","DOIUrl":"https://doi.org/10.1002/advs.202509860","url":null,"abstract":"Cells effectively balance and integrate numerous pathways to adapt to external signals in an attempt to regain homeostasis, although the complex nuclear mechanotransduction mechanism through which this occurs is not as yet fully understood. Contrary to prevalent thought that the relay of extracellular cues to the nucleus to effect its fate and function predominantly relies on direct transmission through the cytoskeletal structure, we demonstrate through the use of high frequency (10 MHz) nanomechanostimulation that induced fluctuations of the cells' nuclear chromatin response are primarily influenced by the spatiotemporal dynamics associated with the bidirectional crosstalk between two key second messengers, namely calcium (Ca2+) and cyclic adenosine monophosphate (cAMP). In particular, this conditioning is shown to be an adaptive response to the mechanostimuli and correlates with a \"mechanopriming\" effect. Notably, brief (10 mins) daily exposure to the mechanostimulation was sufficient to direct mesenchymal stem cells toward an osteogenic lineage in as little as three days-without the need for osteogenic factors.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e09860"},"PeriodicalIF":14.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tumor Intrinsic METTL5 Modulates ATF4 Translation to Prevent T Cell-Induced Ferroptosis in Ovarian Cancer. 肿瘤内生性METTL5调节ATF4翻译预防卵巢癌T细胞诱导的铁下垂。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-10-03 DOI: 10.1002/advs.202507718

Jiakai Hou, Cheng-Wei Ju, Nicholas A Egan, Yanjun Wei, Yunfei Wang, Minghao Dang, Tianyi Zhou, Leilei Shi, Ningbo Zheng, Si Chen, Ashley M Guerrero, Xiaofang Liang, Wanfu Wu, Areej Akhtar, Chitra Dhiman, Debanwita Roy Burman, Andro E Gerges, Mason D Flores, Han Li, Li-Sheng Zhang, Marleen Kok, Xiaobo Mao, Linghua Wang, Qin Feng, Yiwen Chen, Sanghoon Lee, Daniel J McGrail, Nidhi Sahni, Chuan He, Amir A Jazaeri, Weiyi Peng

{"title":"Tumor Intrinsic METTL5 Modulates ATF4 Translation to Prevent T Cell-Induced Ferroptosis in Ovarian Cancer.","authors":"Jiakai Hou, Cheng-Wei Ju, Nicholas A Egan, Yanjun Wei, Yunfei Wang, Minghao Dang, Tianyi Zhou, Leilei Shi, Ningbo Zheng, Si Chen, Ashley M Guerrero, Xiaofang Liang, Wanfu Wu, Areej Akhtar, Chitra Dhiman, Debanwita Roy Burman, Andro E Gerges, Mason D Flores, Han Li, Li-Sheng Zhang, Marleen Kok, Xiaobo Mao, Linghua Wang, Qin Feng, Yiwen Chen, Sanghoon Lee, Daniel J McGrail, Nidhi Sahni, Chuan He, Amir A Jazaeri, Weiyi Peng","doi":"10.1002/advs.202507718","DOIUrl":"https://doi.org/10.1002/advs.202507718","url":null,"abstract":"Poor clinical responses to immune checkpoint blockade (ICB) observed in ovarian cancer (OC) highlight an unmet need to understand the mechanisms driving immune evasion in this disease. To address this, an integrative analysis is conducted by combining in vitro genome-wide immune screens, in vivo ICB screens, and clinical data mining, and METTL5 is identified as a crucial OC-intrinsic factor that promotes immune resistance. Immunologically \"cold\" OC tumors and poor responders to ICB exhibit elevated METTL5 expression. Mechanistically, knocking out (KO) METTL5 in OC disrupts ATF4 translation by altering 18S rRNA m6A levels, leading to the downregulation of SLC7A11 and SLC3A2, whose function is to suppress ferroptosis activity. Consequently, METTL5 KO enhances tumor sensitivity to T cell-mediated antitumor immunity. Notably, the immune-sensitive phenotypes seen in METTL5-KO tumors can be reversed by either ATF4 overexpression or ferroptosis inhibition. These findings underscore the central role of the METTL5/ATF4/ferroptosis axis in controlling OC responses to immunotherapy.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e07718"},"PeriodicalIF":14.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering Dimensional Configuration of Single-Atom S-Cu-S Sites as Reversible Electron Station for Enhanced Peroxidase-Mimicking. 单原子S-Cu-S位点作为增强过氧化物酶模拟的可逆电子站的工程尺寸构型。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-10-03 DOI: 10.1002/advs.202510133

Wenjie Ma, Qian He, Jiancheng Sun, Yiqing Chen, Hongfei Su, Ludan Zhang, Xiao He, Yuguang Wang, Changjian Xie, Zhiyong Zhang, Xin Zhou, Yuliang Zhao, Wenyan Yin

{"title":"Engineering Dimensional Configuration of Single-Atom S-Cu-S Sites as Reversible Electron Station for Enhanced Peroxidase-Mimicking.","authors":"Wenjie Ma, Qian He, Jiancheng Sun, Yiqing Chen, Hongfei Su, Ludan Zhang, Xiao He, Yuguang Wang, Changjian Xie, Zhiyong Zhang, Xin Zhou, Yuliang Zhao, Wenyan Yin","doi":"10.1002/advs.202510133","DOIUrl":"https://doi.org/10.1002/advs.202510133","url":null,"abstract":"Boosting catalytic activity of single-atom nanozymes (SAzymes) to substitute natural metalloenzymes remains challenging due to the lack of enzyme-like secondary building blocks and proper 3D conformation. Herein, a natural amino acid L-cysteine (L-Cys)-triggered auto-assembly process engineers the spatial positioning of 3D-biomimetic S-Cu-S single-atom catalytic sites and adjacent L-Cys on sheet-like MoS2 nanozyme, achieving activated MoCC SAzymes. MoCC achieves a maximum Cu single-atom loading of 10.11% by suppressing aggregation through L-Cys coordination. Particularly, MoCC can properly bind and react with the H2O2 substrate, mimicking 3D catalytic pockets of natural enzymes. The maximum reaction velocity (4.56×10-7 M s-1), affinity (Michaelis constant, 0.65 mM), and specific activity (SA) (355.59 U mg-1) catalyzed by peroxidase (POD)-mimicking MoCC are 16.3-, 17.9-, and 1.2-fold higher than natural horseradish peroxidase (HRP). Density functional theory computations reveal that the S-Cu-S single-atom catalytic sites stabilized by L-Cys bonding function as a reversible electron flow workstation, triggering storage and transfer with MoS2, facilitating swift electron exchange with H2O2, reducing energy barrier for hydroxyl radicals generation. The optimized 3D S-Cu-S single-atom featuring L-Cys building of MoCC exhibits cascaded catalase-like activity and sono-piezocatalysis effect, non-invasively amplifying the generation of oxygen and singlet oxygen. Consequently, multiple free radicals can selectively eliminate dental bacteria and biofilms.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e10133"},"PeriodicalIF":14.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trimetallic Nanozyme-Embedded Smart Hydrogel Enables NIR-Controlled Bacterial Killing and Oxidative Stress Alleviation. 三金属纳米酶嵌入智能水凝胶使nir控制的细菌杀死和氧化应激缓解。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-10-03 DOI: 10.1002/advs.202512875

Zehui Xiao, Jiangli Cao, Jifeng Liu, Zhiyong Song, Ting Du, Xinjun Du

{"title":"Trimetallic Nanozyme-Embedded Smart Hydrogel Enables NIR-Controlled Bacterial Killing and Oxidative Stress Alleviation.","authors":"Zehui Xiao, Jiangli Cao, Jifeng Liu, Zhiyong Song, Ting Du, Xinjun Du","doi":"10.1002/advs.202512875","DOIUrl":"https://doi.org/10.1002/advs.202512875","url":null,"abstract":"Nanozyme-based antibacterial therapy is limited by inefficient single-component nanozymes and complex infection microenvironments. A mild near infrared-I (NIR-I) photothermal-enhanced nanozyme catalytic system is developed using polymyxin B-modified trimetallic nanoparticles (AuMnCu) embedded in a smart hydrogel (AMCB-FTB) formed by 3-formylphenylboronic acid (FPBA), tobramycin (TOB), and tannic acid (TA). The AuMnCu nanozymes exhibit self-switching multi-enzyme activity, generating ROS for bacterial killing in non-NIR mode while scavenging ROS and producing oxygen post-disinfection to alleviate oxidative stress and hypoxia, promoting wound healing. Under NIR-I irradiation, mild hyperthermia (≈44.3 °C) further boosts catalytic activity, enhancing sterilization. The AMCB-FTB hydrogel is injectable, pH-/temperature-responsive, and releases tobramycin/tannic acid in acidic infection microenvironments, synergizing with photothermal therapy (PTT) and nanozyme activity for potent antibacterial effects. In vitro and in vivo studies confirm AMCB-FTB's programmable antibacterial, anti-inflammatory, and pro-regenerative functions via microenvironment self-regulation. RNA sequencing analysis confirm that AMCB-FTB combined with NIR disrupts bacterial energy metabolism, protein synthesis, and lipid pathways, effectively suppressing survival, motility, biofilm formation, and virulence. This work reports a microenvironment-responsive hydrogel with enzyme-mimetic ROS modulation properties, providing a novel pathway to develop thermal-enhanced catalytic materials for refractory diabetic wounds and infectious diseases.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e12875"},"PeriodicalIF":14.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single Cell and Spatial Transcriptomics Define a Proinflammatory and Profibrotic Niche After Kidney Injury. 单细胞和空间转录组学定义肾损伤后的促炎和纤维化生态位。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-10-03 DOI: 10.1002/advs.202503691

Li Li, Jinlin Liao, Yuxi Zhang, Zifu Yao, Junxin Huang, Kejia Wu, Lu Li, Yiling Peng, Haili Zhu, Xue Hong, Xi Liu, Lili Zhou, Fan Fan Hou, Haiyan Fu, Youhua Liu

{"title":"Single Cell and Spatial Transcriptomics Define a Proinflammatory and Profibrotic Niche After Kidney Injury.","authors":"Li Li, Jinlin Liao, Yuxi Zhang, Zifu Yao, Junxin Huang, Kejia Wu, Lu Li, Yiling Peng, Haili Zhu, Xue Hong, Xi Liu, Lili Zhou, Fan Fan Hou, Haiyan Fu, Youhua Liu","doi":"10.1002/advs.202503691","DOIUrl":"https://doi.org/10.1002/advs.202503691","url":null,"abstract":"Kidney fibrosis is the common outcome of chronic kidney disease (CKD). It often instigates in the focal sites by forming the fibrogenic niche after injury. In this study, using single-cell RNA sequencing (scRNA-seq) and a spatial transcriptomic (ST) approach, the cellular heterogeneity, spatial organization, and molecular interactions are delineated in the fibrotic kidney. Through analyses of the scRNA-seq and ST data from normal and fibrotic kidneys in mice subjected to unilateral ischemia-reperfusion injury, a tenascin C (TNC)-enriched, proinflammatory, and profibrotic microenvironment is identified that facilitated macrophage activation and promoted renal inflammation and fibrosis. Both TNC-enriched decellularized kidney tissue scaffold and exogenous TNC protein promoted bone marrow-derived macrophages activation though Toll-like receptor 4 (TLR4)/NF-κB signaling. Either pharmacological inhibition of TLR4 signaling or genetic knockout of its gene alleviated renal inflammation and fibrosis by inhibiting macrophage activation in vivo. Finally, chimeric mice that received bone marrow transplantation from TLR4-deficient donors are protected against kidney inflammation and fibrosis. These results suggest that TNC plays a crucial role in orchestrating the formation of a proinflammatory and profibrotic niche that promotes renal inflammation and fibrosis by activating macrophages via TLR4/NF-κB signaling. The findings underscore the complex interplay among fibroblasts, extracellular microenvironment, and macrophages that drive kidney fibrosis.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e03691"},"PeriodicalIF":14.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intravenous iRGD-Guided, RBC-Membrane Camouflaged Lactococcus Lactis Remodels Cold NSCLC and Enhances PD-1 Blockade. 静脉irgd引导，红细胞膜伪装乳酸乳球菌重塑冷NSCLC并增强PD-1阻断

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-10-03 DOI: 10.1002/advs.202509604

Chen, Junmeng, Xiao Liu, Jie Shao, Aoxing Chen, Yi Mei, Xinyin Zhang, Qinyi Chen, Lin Li, Baorui Liu

{"title":"Intravenous iRGD-Guided, RBC-Membrane Camouflaged Lactococcus Lactis Remodels Cold NSCLC and Enhances PD-1 Blockade.","authors":"Chen, Junmeng, Xiao Liu, Jie Shao, Aoxing Chen, Yi Mei, Xinyin Zhang, Qinyi Chen, Lin Li, Baorui Liu","doi":"10.1002/advs.202509604","DOIUrl":"https://doi.org/10.1002/advs.202509604","url":null,"abstract":"Resistance to programmed-death-1/programmed-death-ligand-1 (PD-1/PD-L1) blockade in non-small-cell lung cancer (NSCLC) arises mainly from weak tumor immunogenicity and limited effector T-cell infiltration. Here, this work presents an intravenously deliverable \"living medicine\" that addresses these barriers through biomimetic cloaking, tumor-penetrating guidance, and synthetic-biology-driven cytokine release. Lactococcus lactis is engineered to co-secrete Flt3L and OX40L (FOLactis) and then camouflage with red-blood-cell membranes, producing long-circulating mRBC@FOLactis. Conjugation of the iRGD peptide (iRGD-mRBC@FOLactis) enables trans-endothelial migration and deep (≥200 µm) interstitial penetration, yielding a fourfold increase in intratumorally bacterial accumulation versus unmodified FOLactis. In the orthotopic Lewis lung carcinoma (LLC) model, a single intravenous dose of iRGD-mRBC@FOLactis combined with anti-PD-1 antibody achieves complete tumor regression in 60% of mice, doubles median survival (p < 0.001), and generates systemic tumor-specific immune memory. Mechanistically, local Flt3L and OX40L secretion expands cross-presenting dendritic cells (DCs), boosts CD8⁺ T-cell priming, and converts immunologically \"cold\" tumors into inflamed, T-cell-rich lesions, thereby overcoming primary resistance to checkpoint blockade. This multifunctional probiotic platform establishes a generalizable strategy for systemic delivery of living therapeutics and offers a powerful adjunct to PD-1/PD-L1 blockade for NSCLC and other treatment-resistant solid tumors.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e09604"},"PeriodicalIF":14.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Throughput Strategies for Streamlining Lipid Nanoparticle Development Pipeline. 精简脂质纳米颗粒开发管道的高通量策略。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-10-03 DOI: 10.1002/advs.202511551

Lois Lam, Stephanie Watson, Yogambha Ramaswamy, Gurvinder Singh

{"title":"High-Throughput Strategies for Streamlining Lipid Nanoparticle Development Pipeline.","authors":"Lois Lam, Stephanie Watson, Yogambha Ramaswamy, Gurvinder Singh","doi":"10.1002/advs.202511551","DOIUrl":"https://doi.org/10.1002/advs.202511551","url":null,"abstract":"Lipid nanoparticles (LNPs) have become clinically validated nanocarriers for nucleic acid delivery, enabling applications in mRNA vaccines and therapies for cancer, ocular, and infectious diseases. Identifying LNPs formulations with optimal physicochemical and pharmacokinetic properties using traditional low-throughput methods is resource-intensive and impractical for evaluating large libraries. Recent advances in automation, high-throughput platforms for lipid synthesis, characterization, and screening tools are transforming the landscape of LNP formulation. These strategies enable rapid multi-parametric generation and evaluation of hundreds to thousands of formulations across key properties such as size, charge, stability, biodistribution, cellular uptake, and intracellular trafficking. In parallel, advanced biomimetic models and in vivo multiplexed barcoding screening strategies provide deeper insights into tissue targeting and therapeutic delivery outcomes. This review provides an integrated framework that combines automation with high-throughput combinatorial synthesis, characterization, and in vitro/in vivo screening tools. In this development pipeline, performance benchmarks applied at each step systematically exclude suboptimal candidates, ensuring that only clinically viable LNP candidates advance. Future directions, including automation, high-throughput, and closed-loop machine learning guided design strategies, are further discussed to advance the development of next-generation LNP therapeutics and accelerate their translation from bench to bedside.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e11551"},"PeriodicalIF":14.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0