Advanced Science最新文献_第2页

cGAS Inhibits ALDH2 to Suppress Lipid Droplet Function and Regulate MASLD Progression. cGAS抑制ALDH2抑制脂滴功能并调节MASLD进展。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-10-03 DOI: 10.1002/advs.202508576

Ying Wang, Yu Deng, Jianfeng Chen, Quentin Hahn, David S Umbaugh, Zhigang Zhang, Yanqiong Zhang, Sarah E Rowe, Lupeng Li, Laura E Herring, Brian P Conlon, Edward A Miao, Blossom Damania, Anna Mae Diehl, Pengda Liu

{"title":"cGAS Inhibits ALDH2 to Suppress Lipid Droplet Function and Regulate MASLD Progression.","authors":"Ying Wang, Yu Deng, Jianfeng Chen, Quentin Hahn, David S Umbaugh, Zhigang Zhang, Yanqiong Zhang, Sarah E Rowe, Lupeng Li, Laura E Herring, Brian P Conlon, Edward A Miao, Blossom Damania, Anna Mae Diehl, Pengda Liu","doi":"10.1002/advs.202508576","DOIUrl":"https://doi.org/10.1002/advs.202508576","url":null,"abstract":"Cyclic GMP-AMP synthase (cGAS) is a cytosolic DNA sensor essential for host defense against microbial infections, but its role beyond innate immunity remains unclear. Here, a non-canonical function of cGAS in regulating aldehyde metabolism and lipid homeostasis is identified. This is demonstrated that cGAS directly binds to and suppresses ALDH2 (aldehyde dehydrogenase 2), a key enzyme in ethanol metabolism and lipid peroxidation. Loss of cGAS activates ALDH2, thereby enhancing ethanol tolerance in mice. Elevated ALDH2 activity upon cGAS loss increases aldehyde conversion into acetyl-CoA, promoting histone acetylation and transcription of lipid synthesis genes, which drives lipid droplet accumulation in cells and in cGas-/- mouse livers. These lipid droplets confer resistance to ferroptosis but simultaneously induce ER stress, impairing STING (stimulator of interferon genes) activation. Functionally, cGas-/- mice fed with a modified high-fat diet develop exacerbated metabolic dysfunction-associated steatotic liver disease (MASLD), characterized by excessive lipid droplet accumulation in livers compared to wild-type controls. In human MASLD patient cohorts, increased cGAS but reduced ALDH2 mRNA expression is observed relative to healthy individuals. Together, this findings uncover a previously unrecognized role of cGAS in metabolic regulation, independent of its innate immune function. By suppressing ALDH2, cGAS controls lipid droplet biogenesis and stress responses, with direct implications for MASLD pathogenesis.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e08576"},"PeriodicalIF":14.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual Regulation of Molecular Rigidity and Orbital Engineering of Pt(II) Emitters for High-Performance Deep-Blue OLEDs. 高性能深蓝oled中Pt（II）发射体分子刚度和轨道工程的双重调控。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-10-03 DOI: 10.1002/advs.202509722

Chengyao Zhang, Kewei Xu, Yun-Fang Yang, Yuanbin She, Guijie Li

{"title":"Dual Regulation of Molecular Rigidity and Orbital Engineering of Pt(II) Emitters for High-Performance Deep-Blue OLEDs.","authors":"Chengyao Zhang, Kewei Xu, Yun-Fang Yang, Yuanbin She, Guijie Li","doi":"10.1002/advs.202509722","DOIUrl":"https://doi.org/10.1002/advs.202509722","url":null,"abstract":"Blue phosphorescent organic light-emitting diodes (PhOLEDs) face critical challenges in terms of low color purity and severe efficiency roll-off. In this study, four novel tetradentate Pt(II) emitters (PtCY, PtCY-F, PtCY-tBu, PtCY-tBuF) are designed through a synergistic strategy of molecular orbital engineering and steric hindrance effect. By introducing a bulky diisopropylbiphenyl (diPrPh) group at the N-heterocyclic carbene (NHC) moiety to increase molecular rigidity and suppress intermolecular interactions, the Pt(II) emitters achieve narrowband deep-blue emission (452-457 nm) in dichloromethane, with full width at half maximum (FWHM) values of 18-23 nm. Combined with the introduction of fluorine atoms (─F) away from the lowest unoccupied molecular orbital (LUMO) distribution, the external quantum efficiency (EQE) is improved while avoiding the breakage of the C─F bond. PtCY-tBuF-based device B4 achieved a high maximum luminance of 45621 cd m-2, and record high EQEs of 27.1%, 24.3%, and 21.7% at high brightness levels of 1000, 5000, and 10 000 cd m- 2 respectively and lowest efficiency roll-off of 3.2% at 1000 cd m-2, among reported Pt(II)-based deep-blue OLEDs with CIEy < 0.15. This study provides a novel strategy for the development of highly efficient tetradentate Pt(II) emitters with high color purity for high-performance deep-blue PhOLED applications.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e09722"},"PeriodicalIF":14.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced Selenium Supplement Extends Lifespan and Delays Multi-Organs Aging by Regulating the Sik1 Pathway Through Maintaining Calcium Homeostasis. 增强硒补充通过维持钙稳态调节Sik1通路延长寿命和延缓多器官衰老。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-10-02 DOI: 10.1002/advs.202511813

Yang Yu, Jintao Song, Mengjiao Guo, RuZe Ma, Mingyang Du, Zhe Xun, Xu Liu, RongXia Xu, Xiaochun Xie, Peilin Qi, Yujie Chen, Dan Shao, Chao Yang, Liang Wang, Xiaoyu Song, Difei Wang

{"title":"Enhanced Selenium Supplement Extends Lifespan and Delays Multi-Organs Aging by Regulating the Sik1 Pathway Through Maintaining Calcium Homeostasis.","authors":"Yang Yu, Jintao Song, Mengjiao Guo, RuZe Ma, Mingyang Du, Zhe Xun, Xu Liu, RongXia Xu, Xiaochun Xie, Peilin Qi, Yujie Chen, Dan Shao, Chao Yang, Liang Wang, Xiaoyu Song, Difei Wang","doi":"10.1002/advs.202511813","DOIUrl":"https://doi.org/10.1002/advs.202511813","url":null,"abstract":"Selenium supplementation has potential in treating aging-related disorders like neurodegenerative and cardiovascular diseases, but its use is limited by poor bioavailability, a narrow therapeutic window, and unclear mechanisms. To overcome this, redox-dual-responsive diselenide-bridged mesoporous silica nanoparticles (SeMSNs) are developed. SeMSNs effectively reduce oxidative stress and downregulate senescence markers (p16, p21), suppressing senescence in both naturally aged primary mouse embryonic fibroblasts (MEFs) and H2O2-induced HEK-293T cells. They show prolonged antioxidant effects (p < 0.05) and lower cytotoxicity (p < 0.01) than commercial selenomethionine. In aged mice, SeMSNs extend lifespan, reduce frailty, and improve age-related conditions, including muscle atrophy, renal dysfunction, cognitive decline, and hepatic steatosis, while restoring metabolic balance. They outperform conventional organically-bridged mesoporous silica nanoparticles (MSNs) and disulfide-bridged MSNs (SMSNs) (p < 0.01). Mechanistically, SeMSNs upregulate selenoproteins (GPx1, SelK), suppress endoplasmic reticulum (ER) stress-mediated calcium release, maintain calcium homeostasis, and inhibit NFATc2-driven Sik1 transcription, reducing p21/p16. Clinical data confirm an inverse correlation between selenium levels and aging biomarkers (p < 0.0001). SeMSNs also restore adipogenic differentiation in human adipose progenitor cells via calcium-NFATc2-Sik1 signaling. These results demonstrate the superiority of SeMSNs over traditional selenium forms, providing a nanotherapeutic strategy to combat multi-organ aging and promote healthy longevity.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e11813"},"PeriodicalIF":14.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-Pot Encapsulation of Enzymes in a Calcium Carboxylate Metal-Organic Framework for Improved Buffer Stability. 一锅包封酶在羧酸钙金属-有机框架提高缓冲稳定性。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-10-02 DOI: 10.1002/advs.202510960

Ying Shu, Weibin Liang, Jun Huang

{"title":"One-Pot Encapsulation of Enzymes in a Calcium Carboxylate Metal-Organic Framework for Improved Buffer Stability.","authors":"Ying Shu, Weibin Liang, Jun Huang","doi":"10.1002/advs.202510960","DOIUrl":"https://doi.org/10.1002/advs.202510960","url":null,"abstract":"Diverse metal-organic frameworks (MOFs) have been actively studied for enzyme encapsulation to enhance encapsulation efficiency (EE), retained enzymatic activity (REA), and stability. This study develops a biocompatible synthesis protocol for a MOF constructed from Ca2⁺ and 4,5-imidazoledicarboxylate ligand (termed CaIDC) in water at room temperature. This method enabled in situ, one-pot encapsulation of various enzymes, including bovine serum albumin (BSA), glucose oxidase (GOx), catalase (CAT), and esterase (EST). Compared to widely studied zeolitic imidazolate frameworks (ZIFs), CaIDC-based biocomposites demonstrated enhanced chemical robustness in phosphate buffer at pH 6.0 and 7.4. To optimize the synthesis process, a machine learning-assisted workflow incorporating Latin hypercube sampling (LHS) is developed for an efficient exploration of the entire synthesis space. As a model system, the optimized EST@CaIDC sample (EC19) exhibited EE, REA, and protein loading (Ploading) values of 28.7%, 20.1%, and 4.2 wt.%, respectively. In all, this study presents the development of a robust CaIDC-based platform for enzyme encapsulation and the implementation of an efficient ML-assisted optimization strategy, offering a pathway to advance enzyme encapsulation technologies with enhanced catalytic performance and sustainability.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e10960"},"PeriodicalIF":14.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deacetylation of ACLY Mediates RNA M⁶A-Modification of NOXA and Promotes Chemoresistance of Colorectal Cancer. ACLY去乙酰化介导NOXA RNA m6a修饰促进结直肠癌化疗耐药

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-10-02 DOI: 10.1002/advs.202503323

Jun Wen, Mengqin Shen, Haitao Zhao, Liu Liu, Qian Hua, Xiaoping Zhao, Jianjun Liu, Haizhong Feng, Gang Huang

{"title":"Deacetylation of ACLY Mediates RNA M6A-Modification of NOXA and Promotes Chemoresistance of Colorectal Cancer.","authors":"Jun Wen, Mengqin Shen, Haitao Zhao, Liu Liu, Qian Hua, Xiaoping Zhao, Jianjun Liu, Haizhong Feng, Gang Huang","doi":"10.1002/advs.202503323","DOIUrl":"https://doi.org/10.1002/advs.202503323","url":null,"abstract":"Chemoresistance is a major challenge for colorectal cancer (CRC) therapy and is a leading cause of cancer mortality, yet the underlying molecular mechanism remains unclear. ATP citrate lyase (ACLY), a rate-limiting enzyme of de novo lipid synthesis, plays an important role in tumor progression and chemotherapy. Here, It is demonstrated that deacetylation of ACLY is critical for chemoresistance in CRC. Through proteomic screening acetylated proteins in chemoresistant patient-derived cells, It is identified that ACLY is deacetylated at K978 site, which induces the relocation of ACLY to the nucleus and promotes its binding to RNA-binding protein 15 (RBM15). This facilitates N6-methyladenosine (m6A) methylation of NOXA (also known as PMAIP1, phorbol-12-myristate-13-acetate-induced protein 1) and decreases the stability of NOXA mRNA, resulting in chemoresistance. With the selective inhibitor Santacruzamate A, targeting the deacetylase histone deacetylase 2 (HDAC2) to inhibit the acetylation may enhance the sensitivity of chemoresistance. These findings provide new insights into the mechanism of ACLY deacetylation promoting chemoresistance and suggest a potential therapeutic strategy to mitigate the chemoresistant effects.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e03323"},"PeriodicalIF":14.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-Level Optical Physical Unclonable Function Based on Random Surface Scattering for Hierarchical Cryptographic Protocols. 基于随机表面散射的分层密码协议多级光学物理不可克隆函数。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-10-02 DOI: 10.1002/advs.202512317

Jeong Jin Kim, Min Seong Kim, Gil Ju Lee

{"title":"Multi-Level Optical Physical Unclonable Function Based on Random Surface Scattering for Hierarchical Cryptographic Protocols.","authors":"Jeong Jin Kim, Min Seong Kim, Gil Ju Lee","doi":"10.1002/advs.202512317","DOIUrl":"https://doi.org/10.1002/advs.202512317","url":null,"abstract":"Optical physical unclonable functions (PUFs) have emerged as a promising cryptographic primitive for next-generation security. However, to harmonize with various modern networks, conventional optical PUF is inadequate due to a rigid key space with a fixed specification. This study implements hierarchically controllable randomness sources for multi-level key generation, exploiting speckle characteristics. By adjusting illumination diameter, the speckle size can be modulated, allowing the extraction of three-level keys with various lengths: 64, 256, and 1,024 bits. Performance evaluation reveals that all levels are superb in uniformity, uniqueness, reproducibility, and randomness. Moreover, it is proposed two feasible applications. The first is a hierarchical authentication architecture that spans from Internet of Things (IoT) devices to sensitive information, balancing security and resources. The second is a multi-stage image encryption algorithm, exhibiting holocryptic performance superior to conventional XOR-based encryption. This versatile platform sets a new paradigm for optical PUF, establishing a foundation for robust and expandable next-generation security.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e12317"},"PeriodicalIF":14.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145204972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autophagy-Targeting Fe-Cu Nanozyme for Tumor Immune Microenvironment Remodeling and Image-Guided Cancer Immunotherapy. 靶向自噬的Fe-Cu纳米酶用于肿瘤免疫微环境重塑和图像引导的癌症免疫治疗。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-10-02 DOI: 10.1002/advs.202512575

Li Yan, Chao Chen, Yu Liang, Xiaowan Huang, Jieying Qian, Hao Zhang, Li Zhang, Yingjia Li, Yunjiao Zhang

{"title":"Autophagy-Targeting Fe-Cu Nanozyme for Tumor Immune Microenvironment Remodeling and Image-Guided Cancer Immunotherapy.","authors":"Li Yan, Chao Chen, Yu Liang, Xiaowan Huang, Jieying Qian, Hao Zhang, Li Zhang, Yingjia Li, Yunjiao Zhang","doi":"10.1002/advs.202512575","DOIUrl":"https://doi.org/10.1002/advs.202512575","url":null,"abstract":"The suppressive tumor immune microenvironment (TIME) is a critical driver of tumor progression, immune evasion, and therapy resistance. Despite the transformative potential of immunotherapy, autophagy within the TIME weakens immune surveillance by downregulating tumor cell surface major histocompatibility complex class I (MHC-I) expression, thereby facilitating immune escape. Here, a novel nanozyme-based strategy is reported to modulate autophagy and restore anti-tumor immunity. Iron-copper metal-organic frameworks (Fe-Cu MOFs) are engineered with tunable peroxidase, glutathione peroxidase, and oxidase-like activities, and an optimal Fe:Cu ratio that confers potent redox activity alongside robust inhibition of autophagic flux is identified. These MOF nanozymes selectively impair autophagy and restore MHC-I expression in tumor cells, enhancing immune recognition. To further potentiate autophagic blockade, a multifunctional nanoplatform (FCMP@CQ/PFH) is developed by co-loading low-dose chloroquine (CQ) and encapsulating perfluorohexane (PFH) into the Fe-Cu MOFs. This combinatorial system couples nanozyme-driven redox stress with lysosomal inhibition to synergistically suppress autophagy and reinvigorate anti-tumor immune responses. Moreover, PFH facilitates ultrasound-based real-time visualization of therapeutic efficacy. Both in vitro and in vivo studies show that FCMP@CQ/PFH enhances cancer immunotherapy and suppresses metastasis. This study establishes a dual-functional approach that combines autophagy inhibition with immune microenvironment reprogramming to circumvent immune resistance and advance precision cancer immunotherapy.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e12575"},"PeriodicalIF":14.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatitis E Virus ORF1 Polyprotein Harbors a Pocket-Like Cavity That Is Vital for Virus Replication and Represents a Novel Antiviral Target. 戊型肝炎病毒ORF1多蛋白含有一个对病毒复制至关重要的口袋状空腔，是一种新的抗病毒靶点。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-10-02 DOI: 10.1002/advs.202501699

Xiaohui Ding, Dou Zeng, Dan Liu, Yingying Bian, Bin Li, Zheng Li, Qiudi Li, Shiquan Liang, Yunlong Si, Qili Yao, Yibo Ding, Jiahui Zhu, Xiangyang Li, Kuiyang Zheng, Hongbo Guo, Wenshi Wang

{"title":"Hepatitis E Virus ORF1 Polyprotein Harbors a Pocket-Like Cavity That Is Vital for Virus Replication and Represents a Novel Antiviral Target.","authors":"Xiaohui Ding, Dou Zeng, Dan Liu, Yingying Bian, Bin Li, Zheng Li, Qiudi Li, Shiquan Liang, Yunlong Si, Qili Yao, Yibo Ding, Jiahui Zhu, Xiangyang Li, Kuiyang Zheng, Hongbo Guo, Wenshi Wang","doi":"10.1002/advs.202501699","DOIUrl":"https://doi.org/10.1002/advs.202501699","url":null,"abstract":"Hepatitis E virus (HEV) is the leading cause of acute viral hepatitis worldwide, yet no FDA-approved anti-HEV medication available. Elucidating HEV replication machinery is therefore crucial for identifying novel antiviral targets, and consequently developing potent antivirals. The nonstructural ORF1 polyprotein is pivotal for HEV replication. Herein, it is revealed that the ORF1 X domain is a critical component of HEV replication machinery. Interestingly, the ADP-ribose hydrolase activity of X domain per se is dispensable for HEV replication. Instead, the X domain supports HEV replication through its interdomain interaction with the RNA-dependent RNA polymerase (RdRp). Structure-based functional analysis reveals that X and RdRp jointly create a \"pocket-like\" cavity (PC) at their interaction interface. Site-directed mutagenesis disrupting the PC integrity completely abolishes HEV replication, demonstrating its crucial role in the viral life cycle. Through a PC-targeted high-throughput in silico screening pipeline, combined with molecular docking analysis, surface plasmon resonance assays, and advanced in vitro HEV models, saikosaponin D and liriopesides B are identified as potent HEV inhibitors targeting this critical interface. Collectively, this study identifies a novel structure within ORF1 polyprotein that is crucial for HEV replication, and demonstrates the feasibility of developing novel antivirals by targeting this PC structure.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e01699"},"PeriodicalIF":14.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

H₂S Is a Potential Universal Reducing Agent for Prx6-Type Peroxiredoxins. H2S是一种潜在的prx6型过氧化物还原剂。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-10-01 DOI: 10.1002/advs.202507214

Lukas Lang, Laura Leiskau, Lea Bambach, Marcel Deponte

{"title":"H2S Is a Potential Universal Reducing Agent for Prx6-Type Peroxiredoxins.","authors":"Lukas Lang, Laura Leiskau, Lea Bambach, Marcel Deponte","doi":"10.1002/advs.202507214","DOIUrl":"https://doi.org/10.1002/advs.202507214","url":null,"abstract":"The absence of a universal reducing agent distinguishes the Prx6-type subfamily of peroxiredoxins from the structurally similar Prx1-type subfamily. A likely explanation for the lack of reactivity of Prx6-type enzymes with common reducing agents is that a histidyl residue at the bottom of the active-site pocket traps the oxidized enzyme in an inaccessible fully-folded protein conformation. Here, we analyzed the reduction of oxidized PfPrx6 from Plasmodium falciparum and human PrxVI by the hydrosulfide ion, HS-, as the smallest possible sulfur-containing universal electron donor. We show that HS- rapidly reacts with oxidized wild-type PfPrx6 or human PrxVI (but not the histidyl mutants PfPrx6H39Y or hPrxVIH39Y) with a second-order rate constant of > 108 m‒1s‒1 at pH 7.4. The obtained protein-hydropersulfide species is neither reduced by thioredoxin nor glutaredoxin and glutathione, but further reacts with an excess of HS- with a second-order rate constant around 104 m‒1s‒1, yielding the reduced enzyme. In summary, we identified HS- as a highly reactive, potential universal electron donor for Prx6-type enzymes. This study marks the starting point for the characterization of the complex reduction pathway of Prx6-type enzymes with implications for H2S detoxification and redox signaling as well as iron-sulfur and persulfide metabolism.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e07214"},"PeriodicalIF":14.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensitive Sub-THz Photodetection in Twisted Graphene with Broad Spectral Response. 具有广谱响应的扭曲石墨烯敏感亚太赫兹光探测。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-09-30 DOI: 10.1002/advs.202512851

Jiaxin Wu, Meiye Hou, Shuangxing Zhu, Jun Cui, Junning Mei, Qi Sun, Yao Wang, Binghe Xie, Kenji Watanabe, Takashi Taniguchi, Zhao Liu, Qi Zhang, Xinghan Cai

{"title":"Sensitive Sub-THz Photodetection in Twisted Graphene with Broad Spectral Response.","authors":"Jiaxin Wu, Meiye Hou, Shuangxing Zhu, Jun Cui, Junning Mei, Qi Sun, Yao Wang, Binghe Xie, Kenji Watanabe, Takashi Taniguchi, Zhao Liu, Qi Zhang, Xinghan Cai","doi":"10.1002/advs.202512851","DOIUrl":"https://doi.org/10.1002/advs.202512851","url":null,"abstract":"The exploitation of photo-induced hot-electron effect in graphene has enabled the advancement of ultrafast photodetectors across the visible to sub-terahertz spectrum. However, the inherent challenges of graphene, including its zero-bandgap, linear dispersion, and atomic-scale thickness, impede the device's photo-electrical conversion efficiency, resulting in a relatively moderate responsivity. Here, monolayer-bilayer graphene into a moiré superlattice is stacked to generate gate-tunable bandgaps and significantly modify the band structure, aiming to enhance the device's performance for sensitive broadband photodetection. The dual-gate twisted monolayer-bilayer graphene (TMBG) transistor exhibits consistent response patterns across the entire spectral range, with the response mechanisms identified as the photothermoelectric effect, observed without a bias voltage, and the bolometric effect, activated by applying bias. At a sub-terahertz frequency of 0.3 THz, the transistor demonstrates exceptional performance at a low temperature of 4.5 K, with an optimized external responsivity of 16.9 A W-1 and a noise equivalent power of 27 fW/Hz1/2 and the operational temperature range can be extended up to room temperature. These findings highlight moiré graphene as a promising platform for the development of high-performance ultra-broadband detectors, particularly in the sub-terahertz domain.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e12851"},"PeriodicalIF":14.1,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0