Biomacromolecules最新文献

Water-Soluble Unconventional Hyperbranched Polyborosiloxane Derivatives for Temperature Sensing in Living Cells.

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-04-08 DOI: 10.1021/acs.biomac.5c00105

Yanyun He, Rui Wu, Zheng Li, Yekun Zhang, Wenyan Liu, Weixu Feng, Hongxia Yan

{"title":"Water-Soluble Unconventional Hyperbranched Polyborosiloxane Derivatives for Temperature Sensing in Living Cells.","authors":"Yanyun He, Rui Wu, Zheng Li, Yekun Zhang, Wenyan Liu, Weixu Feng, Hongxia Yan","doi":"10.1021/acs.biomac.5c00105","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00105","url":null,"abstract":"Fluorescent polymeric thermometers, despite their noninvasive detection and rapid response for intracellular temperature monitoring, face challenges in achieving excellent biocompatibility and high sensitivity. Herein, we synthesized a water-soluble unconventional temperature-sensitive fluorescent polymer (P2) through terminally grafting poly(N-vinylcaprolactam) (PNVCL) onto hyperbranched polyborosiloxane (P1). The P2 exhibited efficient red-light emission and good photostability. Particularly, when the temperature rises, the PNVCL units transform from hydrophilic to hydrophobic, resulting in the dislocation of local segments of P2, suppressing radiative transitions and simultaneously weakening its through-space conjugation, further reducing its fluorescence intensity, and endowing the P2 with a high temperature-sensing sensitivity of 10.06% °C-1. Finally, the real-time monitoring of intracellular temperature variation was further conducted. This work not only develops promising thermochromic materials for intracellular temperature sensing but also provides further insight into the temperature-sensing mechanism of unconventional fluorescent polymers.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymeric Properties of Telomeric G-Quadruplex Multimers: Effects of Chemically Inert Crowders.

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-04-08 DOI: 10.1021/acs.biomac.5c00176

Deniz Mostarac, Mattia Trapella, Luca Bertini, Lucia Comez, Alessandro Paciaroni, Cristiano De Michele

引用次数: 0

Balancing Permeability and Stability: A Study of Hybrid Membranes for Synthetic Cells Using Lipids and PBd-b-PEO Block Copolymers.

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-04-08 DOI: 10.1021/acs.biomac.4c01651

Caterina Presutti, Edo Vreeker, Sajitha Sasidharan, Zanetta Ferdinando, Marc Stuart, Joanna Juhaniewicz-Dębińska, Giovanni Maglia, Wouter H Roos, Bert Poolman

{"title":"Balancing Permeability and Stability: A Study of Hybrid Membranes for Synthetic Cells Using Lipids and PBd-b-PEO Block Copolymers.","authors":"Caterina Presutti, Edo Vreeker, Sajitha Sasidharan, Zanetta Ferdinando, Marc Stuart, Joanna Juhaniewicz-Dębińska, Giovanni Maglia, Wouter H Roos, Bert Poolman","doi":"10.1021/acs.biomac.4c01651","DOIUrl":"https://doi.org/10.1021/acs.biomac.4c01651","url":null,"abstract":"We have synthesized hybrid membranes composed of amphiphilic block copolymers, polybutadiene-poly(ethylene oxide) [PBd-b-PEO], with different lengths [PBd22-PEO14 and PBd11-PEO8] and mixtures of phospholipids (DOPC:DOPG:DOPE 50:25:25 mol %) to combine the properties of both in terms of stability and fluidity of the membrane. The amphiphilic block copolymers increase the stability, whereas the lipids support the functionality of membrane proteins. The hybrid nature of the bilayers was studied by means of Cryo-TEM, Langmuir-Blodgett technique, atomic force microscopy (AFM), electrical measurements, and fluorescence-based stopped-flow assay to determine the permeability of the membrane for water and osmolytes. We observe that the structural, thermodynamic, and permeability properties of hybrid PBd11-PEO8 membranes are similar to their purely lipid counterparts, with the advantage of being more stable and resisting a higher transmembrane electrical potential. Hybrid membranes with the longer polymer, PBd22-PEO14, display more significant structural, thermodynamic, and permeability differences and show less favorable properties than hybrid-PBd11-PEO8 membranes.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Localized Cancer Treatment Using Thiol-Ene Hydrogels for Dual Drug Delivery.

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-04-08 DOI: 10.1021/acs.biomac.5c00387

Lakshmi Sathi Devi, Maria Rosa Gigliobianco, Serena Gabrielli, Dimitrios Agas, Maria Giovanna Sabbieti, Maria Beatrice Morelli, Consuelo Amantini, Cristina Casadidio, Piera Di Martino, Roberta Censi

{"title":"Localized Cancer Treatment Using Thiol-Ene Hydrogels for Dual Drug Delivery.","authors":"Lakshmi Sathi Devi, Maria Rosa Gigliobianco, Serena Gabrielli, Dimitrios Agas, Maria Giovanna Sabbieti, Maria Beatrice Morelli, Consuelo Amantini, Cristina Casadidio, Piera Di Martino, Roberta Censi","doi":"10.1021/acs.biomac.5c00387","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00387","url":null,"abstract":"Combinatorial cancer therapy benefits from injectable hydrogels for localized, controlled drug delivery. This study presents a thiol-ene conjugated hydrogel formed by cross-linking thiol-modified hyaluronic acid (HASH) with vinyl sulfone-modified β-cyclodextrin (CDVS). Four formulations (23Gel-16, 23Gel-33, 99Gel-16, 99Gel-33) were synthesized by varying HASH molecular weight (23 or 99 kDa) and CDVS modification (16% or 33%). Rheological analysis confirmed enhanced viscoelasticity with increasing molecular weight and modification (99Gel-33 > 99Gel-16 > 23Gel-33 > 23Gel-16). The system enabled combinatorial delivery of doxorubicin (DOX) and carvacrol (CRV), exhibiting tumor-responsive degradation and tunable release. DOX release accelerated under tumor-mimicking conditions (100% in 46 h vs 58.7% in PBS), while CRV showed an initial burst followed by sustained release. The hydrogel promoted mesenchymal stem cell proliferation and effectively inhibited triple-negative breast cancer cells. This injectable, tumor-responsive hydrogel system offers a promising platform for minimally invasive, personalized cancer therapy.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Switchable Nanophotosensitizers as Pyroptosis Inducers for Targeted Boosting of Antitumor Photoimmunotherapy.

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-04-08 DOI: 10.1021/acs.biomac.5c00140

Xiaoxi Zhao, Qinjie Zhong, Naibijiang Abudouaini, Yan Zhao, Jibin Zhang, Guozhu Tan, Guifeng Miao, Xiaowu Wang, Jianqiang Liu, Ying Pan, Xiaorui Wang

{"title":"Switchable Nanophotosensitizers as Pyroptosis Inducers for Targeted Boosting of Antitumor Photoimmunotherapy.","authors":"Xiaoxi Zhao, Qinjie Zhong, Naibijiang Abudouaini, Yan Zhao, Jibin Zhang, Guozhu Tan, Guifeng Miao, Xiaowu Wang, Jianqiang Liu, Ying Pan, Xiaorui Wang","doi":"10.1021/acs.biomac.5c00140","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00140","url":null,"abstract":"Photodynamic therapy (PDT) has emerged as a promising modality for cancer treatment, but its clinical application is constrained by unexpected phototoxicity arising from nonspecific photosensitizer activation and their \"always-on\" nature. Herein, we developed a switchable nanophotosensitizer, poly(cation-π) nanoparticles (NP), which achieves supramolecular assembly through cation-π interactions. By coupling choline cationic moieties with aromatic photosensitizers (ZnPc), the polymer facilitates self-assembly driven by cation-π interactions for NP engineering. Surprisingly, the photoactivity of ZnPc was completely quenched upon complexation via cation-π interactions, thereby significantly avoiding skin phototoxicity. Upon targeting tumor cells, NP undergoes a GSH-responsive degradation process that weakens cation-π interactions, leading to spontaneous restoration of photoactivity and amplifying tumor immunogenic pyroptosis. In vivo studies demonstrated that NP achieved a high tumor inhibition rate of 84% while effectively avoiding skin phototoxicity. This work provides a novel perspective for enhancing the safety and efficacy of PDT-based tumor treatment.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and Characterization of Free Radical Scavenging Dendrimer Nanogels via Cross-Linking Reaction-Enabled Flash Nanoprecipitation.

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-04-07 DOI: 10.1021/acs.biomac.5c00050

Lin Qi, Da Huang, Huari Kou, Anna Chernatynskaya, Nuran Ercal, Hu Yang

{"title":"Synthesis and Characterization of Free Radical Scavenging Dendrimer Nanogels via Cross-Linking Reaction-Enabled Flash Nanoprecipitation.","authors":"Lin Qi, Da Huang, Huari Kou, Anna Chernatynskaya, Nuran Ercal, Hu Yang","doi":"10.1021/acs.biomac.5c00050","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00050","url":null,"abstract":"This work reports the development and evaluation of dendrimer-based nanogels based on polyamidoamine (PAMAM) dendrimer generation 5, engineered to act as a carrier with reactive oxygen species (ROS)-scavenging capabilities. We developed a cross-linking reaction-enabled flash nanoprecipitation method in which the cross-linking reaction occurs during the flash nanoprecipitation process to form a cross-linked nanostructure. Using this approach, an N-hydroxysuccinimide (NHS)-functionalized ROS-responsive thioketal cross-linker (TK-NHS) was synthesized and utilized to cross-link DAB-core PAMAM dendrimer G5, resulting in the formation of G5-TK nanogels. The resulting nanogels were characterized using dynamic light scattering and transmission electron microscopy, and their cytocompatibility, irritancy, cellular uptake, and ROS scavenging activity were assessed. We confirmed the ROS scavenging capability of these nanogels and observed favorable safety profiles. The G5-TK nanogels can be further developed as carriers for therapeutic delivery applications to treat oxidative stress-related pathological conditions.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PLA Block Polymers: Versatile Materials for a Sustainable Future.

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-04-07 DOI: 10.1021/acs.biomac.5c00161

Daniel M Krajovic, Margaret S Kumler, Marc A Hillmyer

{"title":"PLA Block Polymers: Versatile Materials for a Sustainable Future.","authors":"Daniel M Krajovic, Margaret S Kumler, Marc A Hillmyer","doi":"10.1021/acs.biomac.5c00161","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00161","url":null,"abstract":"Block polymers present an almost endless realm of possibilities to develop functional materials for myriad applications. The established self-assembly of block polymers allows researchers to access properties that are inaccessible in homopolymers. However, there is a need to develop more sustainable options than the current commodity block polymers. Derived from renewable resources and industrially compostable, poly(lactide) (PLA) is at the forefront of technological advancements in sustainable block polymers. Its material properties including high stiffness, relatively high glass transition temperature, and semicrystallinity in isotactic versions lend themselves to many applications, and its ease of synthesis provides a well-established platform for developing high-performance materials. This Perspective highlights recent advancements associated with PLA-containing block polymers, including their syntheses, mesostructural considerations, and mechanical properties, from resilient elastomers to tough plastics. We also give our perspective on the subfield of PLA block polymers, our outlook on the future, and our assessment of exciting developments yet to come.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autonomous Abiotic Thermal Protectant for Immunoglobulin G: Reducing the Need for Cold Chain Storage.

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-04-07 DOI: 10.1021/acs.biomac.4c01492

Beverly Chou, Rishad J Dalal, Kenneth J Shea

引用次数: 0

Fabrication of Flexible Thin Films from Sodium Alginate Biopolymer Composites for Smart Wearable Sensors Targeting Respiratory Health.

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-04-07 DOI: 10.1021/acs.biomac.5c00179

Nurdiana Nordin, Mun Lie Loke, Azzuliani Supangat, Nurfarhanim Abu Bakar

{"title":"Fabrication of Flexible Thin Films from Sodium Alginate Biopolymer Composites for Smart Wearable Sensors Targeting Respiratory Health.","authors":"Nurdiana Nordin, Mun Lie Loke, Azzuliani Supangat, Nurfarhanim Abu Bakar","doi":"10.1021/acs.biomac.5c00179","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00179","url":null,"abstract":"Modern wearable sensors for health monitoring are becoming increasingly popular due to their versatility. This study investigates the effects of Co2+ ions in flexible alginate-based composite films with doped cobalt and poly(3,4-ethylene-dioxythiophene):polystyrenesulfonate (PEDOT:PSS) for respiratory health monitoring. Contrary to expectations, the Co2+-doped Na-Alg/PEDOT:PSS/Glycerol 3b nanocomposite showed lower LED intensity (168 ± 75 au) and conductivity (5.04 × 10-5 S/m). The addition of Co2+ ions negatively affected the electrical performance and hindered the charge mobility and structural integrity. In contrast, the Na-Alg/PEDOT:PSS/glycerol 2b film achieved higher LED intensity (236 ± 25 au) and maximum conductivity (6.61 × 10-5 S/m), which can be attributed to the plasticizing effect of glycerol that improves the homogeneity of the film and charge transport. This composite also shows excellent wearability on the skin, pressure sensitivity, and the ability to monitor respiration.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and Production of Geranylated Cyclic Peptides by the RiPP Enzymes SyncM and PirF.

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-04-06 DOI: 10.1021/acs.biomac.5c00260

Yanli Xu, Fleur Ruijne, Manel Garcia Diez, Jorrit Jilles Stada, Oscar P Kuipers

引用次数: 0