Biomacromolecules最新文献

Ethylene Glycol and Trehalose Cryopreservation of Cell-Laden Hydrogel Microspheres Enabled by Microfluidic Fabrication. 乙二醇和海藻糖在微流控制备下对载细胞水凝胶微球的低温保存。

IF 5.4 2区化学

Biomacromolecules Pub Date : 2025-10-15 DOI: 10.1021/acs.biomac.5c00559

Jiangnan Yu, Pengfei Pan, Xiaoli Li, Jin Zhang, Xintian Dingzhang, Xia Jiang, Xiaowen Wang, Jollibekov Berdiyar, Qilong Wang, Ximing Xu, Xia Cao

{"title":"Ethylene Glycol and Trehalose Cryopreservation of Cell-Laden Hydrogel Microspheres Enabled by Microfluidic Fabrication.","authors":"Jiangnan Yu, Pengfei Pan, Xiaoli Li, Jin Zhang, Xintian Dingzhang, Xia Jiang, Xiaowen Wang, Jollibekov Berdiyar, Qilong Wang, Ximing Xu, Xia Cao","doi":"10.1021/acs.biomac.5c00559","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00559","url":null,"abstract":"Cell-laden hydrogel microspheres have gained significant attention in 3D cell culture applications, yet effective cryopreservation methods for these systems remain underexplored. This study developed a microfluidic platform for fabricating monodisperse, cell-laden microspheres and investigated a dimethyl sulfoxide (DMSO)- and fetal bovine serum (FBS)-free cryopreservation approach. The platform enabled rapid production of gelatin methacryloyl (GelMA) and calcium alginate (ALG) microspheres, demonstrating cell viability exceeding 80% for U251 cells in GelMA microspheres and 90% for both U251 cells and induced pluripotent stem cells (iPSCs) in ALG microspheres. A DMSO-/FBS-free cryoprotectant (12% ethylene glycol, 4% trehalose; E/T) was identified that maintained >90% post-thaw viability in GES, U251, HepG2, A549, and 3T6 cells, with iPSCs retaining >80% viability. Crucially, E/T effectively preserved iPSC-laden microspheres while preventing DMSO-induced apoptosis and preserving pluripotency. This work establishes a systematic protocol for cryopreserving cell-laden hydrogel microspheres without DMSO/FBS, providing a clinically translatable strategy to advance 3D cell culture technologies.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyamine-Modified Poly(aspartic acid) for mRNA Delivery with In Vivo Lung-Targeted Ability. 聚胺修饰的聚天冬氨酸用于mRNA的体内肺靶向递送。

IF 5.4 2区化学

Biomacromolecules Pub Date : 2025-10-15 DOI: 10.1021/acs.biomac.5c00802

Yuyan Zhang, Pingjie Sun, Sheng Ma, Zhaopei Guo, Hanqin Zhao, Yibo Qi, Minhui Li, Wantong Song, Zhaohui Tang

{"title":"Polyamine-Modified Poly(aspartic acid) for mRNA Delivery with In Vivo Lung-Targeted Ability.","authors":"Yuyan Zhang, Pingjie Sun, Sheng Ma, Zhaopei Guo, Hanqin Zhao, Yibo Qi, Minhui Li, Wantong Song, Zhaohui Tang","doi":"10.1021/acs.biomac.5c00802","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00802","url":null,"abstract":"Polymers are widely used as mRNA delivery platforms, but their clinical translation is limited by challenges such as nonorgan-selective expression and low in vivo efficacy. Poly(amino acids), particularly poly(aspartic acid) (PAsp), have been extensively studied for drug, nucleic acid, and protein delivery due to their excellent biodegradability and biocompatibility. However, the role of aminolysis-modified PAsp in mRNA delivery remains to be fully explored. In this study, we developed a series of polyamine-aminolyzed PAsp derivatives (P-An), further functionalized with heterocyclic small molecules (P-An-M), and evaluated their in vitro and in vivo mRNA transfection efficiency. We synthesized 24 polymers and identified three N,N'-bis(3-aminopropyl)ethylenediamine (PDA)-modified PAsp derivatives that efficiently transfected Luc-mRNA in 293T cells: P-PDA, P-PDA-I, and P-PDA-BI (where I and BI represent 1H-imidazole-4-carboxylic acid and 1H-benzimidazole-4-carboxylic acid, respectively). In vivo experiments demonstrated that P-PDA, P-PDA-I, and P-PDA-BI selectively delivered mRNA to the lungs and achieved a significant level of protein expression. This work provides a promising strategy for developing polymer-based materials for mRNA lung therapy, with potential applications in treating pulmonary diseases.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conducting Polysaccharide Hydrogel with near-Linear Ultrastrain, Self-Adhesion, and Self-Healing Modulated by Molecular Engineering of Dissipative Energy for Human-Machine Interaction. 人机交互耗散能分子工程调控的近线性超应变自粘自愈导电多糖水凝胶。

IF 5.4 2区化学

Biomacromolecules Pub Date : 2025-10-15 DOI: 10.1021/acs.biomac.5c01333

Wendong Zhu, Shikun Chen, Zecheng Tao, Yang Liu, Ce Wang, Ya Cheng

{"title":"Conducting Polysaccharide Hydrogel with near-Linear Ultrastrain, Self-Adhesion, and Self-Healing Modulated by Molecular Engineering of Dissipative Energy for Human-Machine Interaction.","authors":"Wendong Zhu, Shikun Chen, Zecheng Tao, Yang Liu, Ce Wang, Ya Cheng","doi":"10.1021/acs.biomac.5c01333","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c01333","url":null,"abstract":"Interface issues between skin and electric device have always been a difficulty, including mechanical property, adhesion, and biocompatibility. Especially mechanical strength and linear deformation are contradiction points. Herein, an ultrastretched, self-healing adhesive and conducting polysaccharide hydrogel with a near-linear deformation is developed via the molecular engineering of dissipative energy strategy. Two physical bonds with a large energy difference are introduced into the hydrogel to realize the near-linear ultratensile. Strong metal coordination provides mechanical strength, while numerous weak hydrogen bonds counteract the yielding, so that near-linear ultradeformation (2199.27%), elastic modulus (29.025 kPa), stress (183.05 kPa), toughness (2.65 MJ m-3), adhesion, and self-healing characterizations are accomplished. Moreover, based on mechanical and adhesive properties, hydrogel-based sensors are fabricated. Furthermore, robotic hand control, assistance driving, and tactile tracing are realized. This work provided a novel and universal approach to design and fabricate an elastomer with near-perfect linear ultradeformation by battle strong and weak interactions for HMI.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solid-State NMR Analysis Aimed at Elucidating the Atomic-Level Structure and Dynamic Behavior of Silk Using Alanine Residues as Markers. 以丙氨酸残基为标记物的固态核磁共振分析旨在阐明蚕丝的原子水平结构和动态行为。

IF 5.4 2区化学

Biomacromolecules Pub Date : 2025-10-14 DOI: 10.1021/acs.biomac.5c01097

Akira Naito, Tetsuo Asakura

{"title":"Solid-State NMR Analysis Aimed at Elucidating the Atomic-Level Structure and Dynamic Behavior of Silk Using Alanine Residues as Markers.","authors":"Akira Naito, Tetsuo Asakura","doi":"10.1021/acs.biomac.5c01097","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c01097","url":null,"abstract":"Understanding the structure, packing, and dynamics of silk fibers from silkworms and spider draglines is essential to explain their excellent mechanical properties. However, their atomic coordinate structural information remains limited. This review focuses on the methyl group of alanine and employs mainly 13C solid-state NMR chemical shifts and spin-lattice relaxation times to elucidate silk structure including the packing structure, and dynamics of alanine methyl groups quantitatively. Wild silkworm (Samia cynthia ricini) silk shows a staggered polyalanine packing, while spider silk exhibits a mixed packing of rectangular and staggered types with glycine-rich segments mainly forming random coils and β-turns. Domestic silkworm silk features an antipolar lamellar structure, folding every eight amino acids via β-turns. Additionally, some alanine Cβ carbons display long relaxation times and short correlation times at short 13C-13C distances, suggesting fast gear-like hopping motion under strong 13C-13C interactions, which stabilizes the staggered stacking of antiparallel β-sheets.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silylated Softwood and Hardwood Lignin: Impact on Thermomechanical and Interfacial Properties of PLA Biocomposites. 硅化软木和硬木木质素：对聚乳酸生物复合材料热力学和界面性能的影响。

IF 5.4 2区化学

Biomacromolecules Pub Date : 2025-10-14 DOI: 10.1021/acs.biomac.5c01179

Giulia Herbst, Gabriela Adriana Bastida, Quim Tarrés, Marcos Lúcio Corazza, Luiz Pereira Ramos, Marc Delgado-Aguilar

{"title":"Silylated Softwood and Hardwood Lignin: Impact on Thermomechanical and Interfacial Properties of PLA Biocomposites.","authors":"Giulia Herbst, Gabriela Adriana Bastida, Quim Tarrés, Marcos Lúcio Corazza, Luiz Pereira Ramos, Marc Delgado-Aguilar","doi":"10.1021/acs.biomac.5c01179","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c01179","url":null,"abstract":"Achieving compatibility between biopolymers and natural fillers is a significant challenge in developing sustainable materials. PLA-lignin biocomposites frequently demonstrate poor interfacial adhesion, mostly due to polarity differences. Softwood (LS) and hardwood (LH) lignins vary in composition and reactivity, affecting PLA structure. This study evaluated the surface compatibilization of LS and LH through silylation at 1, 3, and 5 wt % using a GPS coupling agent. Silylation was validated by TGA, DSC (Tg increase of ∼3-7 °C), ash color, and EDX (increased silicon). FTIR assessed structural differences in lignins. Rheological tests and melt flow index indicated that unmodified lignin reduced PLA viscosity, while GPS-modified lignin increased it. DSC showed that LS enhanced crystallization more than LH, and GPS at 1 wt % promoted nucleation. Films containing LH at 10 and 1 wt % GPS exhibited improved mechanical properties. Barrier properties remained unchanged, though all films provided UV-blocking capability.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A 3D Bioprinted Pancreatic Cancer Model Using Collagen-Gelatin Methacrylamide-Alginate Bioinks to Mimic the Desmoplastic Microenvironment. 使用胶原-明胶-甲基丙烯酰胺-海藻酸盐生物墨水模拟促结缔组织微环境的3D生物打印胰腺癌模型。

IF 5.4 2区化学

Biomacromolecules Pub Date : 2025-10-14 DOI: 10.1021/acs.biomac.5c00450

Uxia Gato-Diaz, Sandra Blanco-Garcia, Diana Peixoto, Angel Concheiro, Carmen Alvarez-Lorenzo, Barbara Blanco-Fernandez

{"title":"A 3D Bioprinted Pancreatic Cancer Model Using Collagen-Gelatin Methacrylamide-Alginate Bioinks to Mimic the Desmoplastic Microenvironment.","authors":"Uxia Gato-Diaz, Sandra Blanco-Garcia, Diana Peixoto, Angel Concheiro, Carmen Alvarez-Lorenzo, Barbara Blanco-Fernandez","doi":"10.1021/acs.biomac.5c00450","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00450","url":null,"abstract":"Pancreatic ductal adenocarcinoma is one of the cancers with the least favorable survival prognosis worldwide. It is characterized by a high desmoplastic stroma rich in collagen I, which regulates pancreatic cancer cells' behavior. There is a critical need to develop desmoplastic 3D models for preclinical testing. In this study, bioinks that imitate the biochemical characteristics of pancreatic ductal adenocarcinoma were developed to observe the influence that the desmoplastic extracellular matrix has on cancer cells. The bioinks were made of gelatin methacrylamide, alginate, and different concentrations of collagen I. Cancer cells were able to proliferate in all bioinks, presenting high paclitaxel resistance and a high expression of desmoplasia and extracellular matrix remodeling markers. The designed bioinks can play a crucial role in developing more clinically relevant cancer models for chemotherapeutic drug screening. Furthermore, they have significant potential for studying the influence of desmoplasia and for improving advanced treatment approaches for pancreatic cancer.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifunctional and UV Light-Triggered Degradable Highly Branched Vinyl Polymer for Efficient mRNA Delivery. 多功能和紫外光触发的可降解高支化乙烯基聚合物用于mRNA的高效传递。

IF 5.4 2区化学

Biomacromolecules Pub Date : 2025-10-14 DOI: 10.1021/acs.biomac.5c00555

Wei Sun, Xiaojia Wang, Chenfei Wang, Zhili Li, Rui Guo, Zhedong Ma, Li Luo, Ming Zeng, Dezhong Zhou

{"title":"Multifunctional and UV Light-Triggered Degradable Highly Branched Vinyl Polymer for Efficient mRNA Delivery.","authors":"Wei Sun, Xiaojia Wang, Chenfei Wang, Zhili Li, Rui Guo, Zhedong Ma, Li Luo, Ming Zeng, Dezhong Zhou","doi":"10.1021/acs.biomac.5c00555","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00555","url":null,"abstract":"Vinyl polymers synthesized via radical polymerization of (meth)acrylates are widely used in various biomedical fields. We developed a multifunctional, highly branched vinyl polymer, HPNDN, with ultraviolet (UV) light-triggered degradability for highly efficient mRNA delivery. (2-Nitro-1,3-phenylene) bis(methylene) diacrylate (NPBMDA) was copolymerized with 2-(dimethylamino)ethyl methacrylate (DMAEMA) and 3,3,4,4,5,5,6,6-nonafluorohexyl acrylate (NFHA) by reversible addition-fragmentation chain transfer (RAFT) polymerization followed by end-capping with ethylenediamine (EDA), resulting in HPNDN with a highly branched topological structure, multiple fluorine moieties, and multiple amines. Upon exposure to UV light irradiation, HPNDN degrades within 5 min through the cleavage of the ester groups in the NPBMDA units. Importantly, HPNDN can effectively condense mRNA to form nanosized polyplexes, achieving high transfection efficiency of up to 87.99%, 62.75% and 52.37% in African green monkey kidney (COS-7) cells, human embryonic kidney (293T) cells, and rat schwann (RSC96) cells, respectively, without inducing obvious cytotoxicity.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prediction of Physical Characteristics of Disordered Proteins Using Molecular Simulation and Physics-Informed Multiple Machine Learning Strategies. 利用分子模拟和物理信息多机器学习策略预测无序蛋白质的物理特性。

IF 5.4 2区化学

Biomacromolecules Pub Date : 2025-10-14 DOI: 10.1021/acs.biomac.5c01118

Diego Linares Gonzalez, Shahana Ibrahim, Swarnadeep Seth, George Atia, Aniket Bhattacharya

{"title":"Prediction of Physical Characteristics of Disordered Proteins Using Molecular Simulation and Physics-Informed Multiple Machine Learning Strategies.","authors":"Diego Linares Gonzalez, Shahana Ibrahim, Swarnadeep Seth, George Atia, Aniket Bhattacharya","doi":"10.1021/acs.biomac.5c01118","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c01118","url":null,"abstract":"We introduce a novel hybrid machine learning (ML) framework to predict the radius of gyration and other conformational properties of intrinsically disordered proteins (IDPs). Our model integrates sequence information with physical features derived from a coarse-grained model validated by experimental data. Specifically, we combine hidden states from sequence-based models with 23 physical features projected into a shared latent space, and apply an attention mechanism that assigns weights to each residue to highlight the most informative regions of the sequence. This attention-guided fusion significantly improves predictive accuracy across multiple metrics, including mean absolute percentage error and mean squared error, while also enhancing confidence in the predictions. We trained and evaluated our models on Brownian dynamics (BD) simulation results for approximately 7000 IDPs from the MobiDB database (each with >99% disorder score). We find that sequence-based models consistently outperform feature-only models, with the GRU achieving the best performance among sequence-only approaches. Moreover, combining sequence and feature information further improves accuracy across all architectures, with the hybrid biGRU model delivering the best overall predictive performance. SHAP analysis reveals the relative importance of physical features, offering model explainability, and guiding feature selection. Notably, using a small number of top features often reduces model complexity and improves generalization. Furthermore, an integrated gradient analysis reveals that in addition to the length of the IDPs, the three parameters (sequence charge and hydropathy decoration parameters (SCD and SHD), and charge asymmetry parameter f*) play a key role in the predictions of ML. Our framework provides a fast, interpretable, and scalable tool for predicting IDP behavior, enabling efficient initial screening prior to costly molecular simulations.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Redox Dextran-Based Self-Adapting Adhesive Hydrogels for Subconjunctival Injury to Alleviate Inflammation and Accelerate Healing. 基于氧化还原右旋糖酐的自适应黏附水凝胶用于结膜下损伤减轻炎症和加速愈合。

IF 5.4 2区化学

Biomacromolecules Pub Date : 2025-10-14 DOI: 10.1021/acs.biomac.5c01399

Boyuan An, Yuting Deng, Haohao Cui, Mengke Wang, Jiake Li, Qin Zeng, Zhanrong Li, Jingguo Li

{"title":"Redox Dextran-Based Self-Adapting Adhesive Hydrogels for Subconjunctival Injury to Alleviate Inflammation and Accelerate Healing.","authors":"Boyuan An, Yuting Deng, Haohao Cui, Mengke Wang, Jiake Li, Qin Zeng, Zhanrong Li, Jingguo Li","doi":"10.1021/acs.biomac.5c01399","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c01399","url":null,"abstract":"Traumatic or chemical injuries to the conjunctival and subconjunctival tissue can lead to defects and associated inflammatory responses, significantly impairing the tissue repair process. Current repair methods lack effective integration of anti-inflammatory action with wound sealing. We combined redox dextran (POD) with poly(vinyl alcohol) (PVA) through physical hydrogen bonds and boronic ester bonds and achieved structural reinforcement through freeze-thawing cycles, developing a novel, self-adapting, anti-inflammatory, dual-network hydrogel adhesive (POAD). The POD surface combines both high degree of freedom primary hydroxyl groups and reactive aldehyde groups, which significantly improves the cohesive and interfacial interactions. In addition, the hydrogel adapts to complex wound shapes and degrades slowly in the presence of reactive oxygen species (ROS), relieving inflammation while making room for tissue regeneration and accelerating the tissue repair process. These properties suggest that POAD hydrogels will be an alternative strategy for future functional ocular surface reconstruction.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nucleophilic Initiation of Episulfide Anionic Ring-Opening Polymerization by Amino Acids and Oligopeptides. 氨基酸和寡肽引发表硫化物阴离子开环聚合的亲核性研究。

IF 5.4 2区化学

Biomacromolecules Pub Date : 2025-10-14 DOI: 10.1021/acs.biomac.5c01251

Emma Mongkhoun, Ons Amamou, Christophe Piesse, Philippe Guégan, Nicolas Illy

{"title":"Nucleophilic Initiation of Episulfide Anionic Ring-Opening Polymerization by Amino Acids and Oligopeptides.","authors":"Emma Mongkhoun, Ons Amamou, Christophe Piesse, Philippe Guégan, Nicolas Illy","doi":"10.1021/acs.biomac.5c01251","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c01251","url":null,"abstract":"Peptide-polymer conjugates are promising molecules because they combine the biological functionality of peptides with the versatile properties of polymers, enabling applications in drug delivery, biomaterials, and nanomedicine with enhanced stability, biocompatibility, and tailored functionalities. Until now, the grafting-to approach has been the predominant strategy for synthesizing these biohybrids. By comparison, grafting-from techniques are relatively less diverse. Mainly, radical grafting-from techniques have also been developed, but they present a number of limitations, including a limited variety of polymerizable monomers and the introduction of nonbiodegradable polymer chains. The AROP-based grafting-from strategy offers a promising, yet still underexplored, route for synthesizing peptide-polymer conjugates bearing heteroatom-containing polymer side chains. We report an original AROP grafting-from strategy using primary amines as attachment sites, N-acetyl homocysteine thiolactone as a linker, and propylene sulfide as a monomer. The grafting-from technique was optimized using various protected and unprotected amino acids as model scaffolds. In addition, the regioselective functionalization of primary amine lateral substituents of lysine residues over chain-end amines was demonstrated. The method was then extended to dipeptides and tripeptides. Surprisingly, the grafting-from polymerization was demonstrated to also occur in a controlled manner using oligopeptide initiators bearing unprotected terminal carboxylic acid end-groups. Furthermore, this technique was applied to the introduction of polythioether grafts on the KLVFF peptide sequence (Lys-Leu-Val-Phe-Phe), which is a key recognition motif found in the amyloid-β (Aβ) protein, a protein strongly associated with Alzheimer's disease.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0