BiomacromoleculesPub Date : 2025-06-09DOI: 10.1021/acs.biomac.5c00228
Armin Amirsadeghi , Shahriar Mahdavi , Paula Jager , Marleen Kamperman , Julien Es Sayed
{"title":"3D-Printable Granular Hydrogel Composed of Hyaluronic Acid-Chitosan Hybrid Polyelectrolyte Complex Microgels","authors":"Armin Amirsadeghi , Shahriar Mahdavi , Paula Jager , Marleen Kamperman , Julien Es Sayed","doi":"10.1021/acs.biomac.5c00228","DOIUrl":"10.1021/acs.biomac.5c00228","url":null,"abstract":"<div><div>When it comes to 3D printing of hydrogels, optimizing rheological properties is crucial to ensure (i) a smooth flow of the ink through the nozzle during printing and (ii) structural integrity postprinting. Granular hydrogels offer excellent printability due to their intrinsic yield-stress properties; however, they typically lack postprinting integrity in aqueous environments. To address this limitation, a novel approach is proposed to integrate the yield-stress behavior of granular hydrogels with polyelectrolyte complexes, which exhibit tunable mechanical properties and structural integrity in aqueous media. In this approach, hybrid microgels composed of chemically co-cross-linked hyaluronic acid–chitosan polyelectrolytes are prepared in a high-salt medium to shield electrostatic interactions and then jammed to form a printable granular hydrogel. By reducing the salt concentration below the critical threshold for electrostatic association, intra- and interparticle electrostatic interactions are activated, causing both the microgels and the granular hydrogel to shrink. This process yields a hydrogel with tunable stiffness, packing density, and dimensions. Overall, this strategy paves the way for the design of 3D hydrogel constructs with dynamic properties.</div></div><div><div><span><figure><span><img><ol><li><span><span>Download: <span>Download high-res image (137KB)</span></span></span></li><li><span><span>Download: <span>Download full-size image</span></span></span></li></ol></span></figure></span></div></div>","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":"26 6","pages":"Pages 3641-3650"},"PeriodicalIF":5.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiomacromoleculesPub Date : 2025-06-09DOI: 10.1021/acs.biomac.5c00494
Jackson Powers , Jooyong Shin , Fatima Rizwan , Seok Hoon Hong , Yeongseon Jang
{"title":"Cell-Free Protein Synthesis of Fusion-Protein Building Blocks Enables Autonomous Growth in Globular Protein Vesicle Protocells","authors":"Jackson Powers , Jooyong Shin , Fatima Rizwan , Seok Hoon Hong , Yeongseon Jang","doi":"10.1021/acs.biomac.5c00494","DOIUrl":"10.1021/acs.biomac.5c00494","url":null,"abstract":"<div><div>The capacity for autonomous synthesis of building blocks and functional molecules is a fundamental feature of living cells. Encapsulating complex synthesis systems, such as cell-free protein synthesis (CFPS), within vesicular platforms has propelled the field toward more intricate biomimicry in artificial cell development, yet synthesizing functional proteins as membrane components remains challenging. In this study, we report the successful integration of a CFPS system with globular protein vesicles (GPVs), a functionally folded protein-assembled vesicular platform. We demonstrate that in vitro transcription and translation of modular fusion proteins, which serve as GPV building blocks, facilitate the direct incorporation of newly synthesized fusion proteins into the vesicle membrane. This system supports the expression of membrane components, enabling GPVs to exhibit autonomous growth. Our approach marks substantial progress in the development of synthetic cells, providing a versatile and robust strategy to expand the available repertoire of biomimetic functions achievable through de novo protein production.</div></div><div><div><span><figure><span><img><ol><li><span><span>Download: <span>Download high-res image (140KB)</span></span></span></li><li><span><span>Download: <span>Download full-size image</span></span></span></li></ol></span></figure></span></div></div>","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":"26 6","pages":"Pages 3879-3887"},"PeriodicalIF":5.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiomacromoleculesPub Date : 2025-06-09DOI: 10.1021/acs.biomac.5c00420
Clemens Krage , Peyman Malek Mohammadi Nouri , Jens Dernedde , Jayachandran N. Kizhakkedathu , Sarah Hedtrich , Rainer Haag , Katharina Achazi
{"title":"Anti-Inflammatory Effects of Polyglycerol Sulfates and Natural Polyanions in Type 2 Inflammation","authors":"Clemens Krage , Peyman Malek Mohammadi Nouri , Jens Dernedde , Jayachandran N. Kizhakkedathu , Sarah Hedtrich , Rainer Haag , Katharina Achazi","doi":"10.1021/acs.biomac.5c00420","DOIUrl":"10.1021/acs.biomac.5c00420","url":null,"abstract":"<div><div>Type 2 inflammation is an essential defense mechanism of the innate and adaptive immune systems, but when dysregulated, it can cause chronic atopic diseases like allergic asthma and atopic dermatitis. Thymic stromal lymphopoietin (TSLP) helps drive type 2 inflammation by guiding T cells toward a type 2 helper cell (T<sub>H</sub>2) subtype and stimulating B cells’ antibody production. Fibronectin (FN) has recently been found at elevated levels in the plasma of children with atopic dermatitis and shown a potential proinflammatory role in bronchial epithelium tissue models. Both proteins’ surface charges suggest potential interaction with charged molecules. Seeking new strategies against type 2 inflammation, we found that negatively charged polyglycerol sulfates strongly bind to TSLP and FN. We confirmed that these molecules inhibit inflammation by reducing the TSLP-mediated type 2 polarization of CD4<sup>+</sup> T cells. We found that adding polyglycerol sulfate to FN-triggered inflamed bronchial epithelium models reduced TSLP expression and interleukin 6 secretion.</div></div><div><div><span><figure><span><img><ol><li><span><span>Download: <span>Download high-res image (101KB)</span></span></span></li><li><span><span>Download: <span>Download full-size image</span></span></span></li></ol></span></figure></span></div></div>","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":"26 6","pages":"Pages 3819-3829"},"PeriodicalIF":5.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144131799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dao Thi Hong Le, Chuan Yang, Yue Zhang, Gui Zhao, Melgious J. Y. Ang, Ki Hyun Bae, James H. P. Hui, James L. Hedrick and Yi Yan Yang*,
{"title":"","authors":"Dao Thi Hong Le, Chuan Yang, Yue Zhang, Gui Zhao, Melgious J. Y. Ang, Ki Hyun Bae, James H. P. Hui, James L. Hedrick and Yi Yan Yang*, ","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":"26 6","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":5.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acs.biomac.5c00088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144358037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiong Xiao, Ziwei Huang, Hongying Duan, Liping Yang, Yuchu Yang, Yushang Lai, Chenxi Li and Li Feng*,
{"title":"","authors":"Xiong Xiao, Ziwei Huang, Hongying Duan, Liping Yang, Yuchu Yang, Yushang Lai, Chenxi Li and Li Feng*, ","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":"26 6","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":5.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acs.biomac.4c01775","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144432362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dominykas Veiveris, Aurimas Kopustas, Darius Sulskis, Kamile Mikalauskaite, Mohammad Nour Alsamsam, Marijonas Tutkus, Vytautas Smirnovas and Mantas Ziaunys*,