Biomacromolecules最新文献_第10页

Programming Aliphatic Polyester Degradation by Engineered Bacterial Spores. 编程脂肪族聚酯降解工程细菌孢子。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-03-10 Epub Date: 2025-02-24 DOI: 10.1021/acs.biomac.4c01652

Ziyu Cui, Masamu Kawada, Yue Hui, Seunghyun Sim

引用次数: 0

3D-Printed Protein-Based Bioplastics with Tunable Mechanical Properties Using Glycerol or Hyperbranched Poly(glycerol)s as Plasticizers. 使用甘油或超支化聚（甘油）作为增塑剂的具有可调机械性能的3d打印蛋白质生物塑料。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-03-10 Epub Date: 2025-02-07 DOI: 10.1021/acs.biomac.4c01497

S Cem Millik, Naroa Sadaba, Shayna L Hilburg, Eva Sanchez-Rexach, Meijing Zhang, Siwei Yu, Alexander F Vass, Lilo D Pozzo, Alshakim Nelson

{"title":"3D-Printed Protein-Based Bioplastics with Tunable Mechanical Properties Using Glycerol or Hyperbranched Poly(glycerol)s as Plasticizers.","authors":"S Cem Millik, Naroa Sadaba, Shayna L Hilburg, Eva Sanchez-Rexach, Meijing Zhang, Siwei Yu, Alexander F Vass, Lilo D Pozzo, Alshakim Nelson","doi":"10.1021/acs.biomac.4c01497","DOIUrl":"10.1021/acs.biomac.4c01497","url":null,"abstract":"Protein-based materials can be engineered to derive utility from the structures and functions of the incorporated proteins. Modern methods of protein engineering bring promise of unprecedented control over molecular and network design, which will enable new and improved functionalities in materials that incorporate proteins as functional building blocks. For these advantages to be fully realized, there is a need for robust methods for producing protein-based networks, as well as methods for tuning their mechanical properties. Light-based 3D-printing techniques afford high-resolution fabrication capability with unparalleled design freedom in an inexpensive and decentralized capacity. This work features 3D-printed serum albumin-based bioplastics with mechanical properties modulated through the incorporation of glycerol or hyperbranched poly(glycerol)s (HPGs) as plasticizers. These materials capitalize upon important features of serum albumin, including its low intrinsic viscosity, high aqueous solubility, and relatively low cost. The incorporation of glycerol or HPGs of different sizes resulted in softer and more ductile bioplastics than those obtained natively without additives. These bioplastics showed shape-memory behavior and could be used to fabricate functional objects. These materials are accessible, possess minimal chemical hazards, and can be used for fabricating rigid and strong as well as soft and ductile parts using inexpensive commercial 3D printers.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":"1725-1736"},"PeriodicalIF":5.5,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tetravalent Virus-like Particles Engineered To Display Envelope Domain IIIs of Four Dengue Serotypes in Silkworm as Vaccine Candidates.

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-03-10 Epub Date: 2025-02-03 DOI: 10.1021/acs.biomac.4c01831

Krishna Raja Muthuraman, Jirayu Boonyakida, Mami Matsuda, Ryosuke Suzuki, Tatsuya Kato, Enoch Y Park

{"title":"Tetravalent Virus-like Particles Engineered To Display Envelope Domain IIIs of Four Dengue Serotypes in Silkworm as Vaccine Candidates.","authors":"Krishna Raja Muthuraman, Jirayu Boonyakida, Mami Matsuda, Ryosuke Suzuki, Tatsuya Kato, Enoch Y Park","doi":"10.1021/acs.biomac.4c01831","DOIUrl":"10.1021/acs.biomac.4c01831","url":null,"abstract":"Dengue virus (DENV) causes dengue fever, the leading mosquito-borne viral disease affecting millions globally. Licensed vaccines have their restrictions, and the development of vaccines is in progress to overcome the limitations. In this study, we expressed two types of virus-like particles (VLPs) and four DENV serotype antigens, 1EDIII-4EDIII (tetEDIII), in silkworm larvae and engineered them into tetravalent VLPs (tetVLPs) displaying tetEDIII. Canine parvovirus-like particles (CPV-LPs) were self-assembled in vivo from viral protein VP2 of CPV (CPV-VP2) as heterologous VLPs; dengue virus capsid-like particles (DENV C-LPs) from capsid protein of DENV serotype 2 (DENV-C2) as homologous VLPs. The tetEDIII was displayed on the surface of CPV-LPs and DENV C-LPs through in vitro SpyTag/SpyCatcher (SpT/SpC) covalent ligation. The EDIII display of CPV-LP is better than that of DENV C-LP. Both tetEDIII-displaying tetravalent CPV-LPs (tetCPV-LPs) and tetravalent DENV C-LPs (tetDENV C-LPs) elicited neutralizing antibodies in BALB/c mice assayed through the single-round infectious particles (SRIP) method. The immunogenicity of tetDENV C-LPs for anti-IgG EDIIIs was higher than that of tetCPV-LPs for serotypes 1 and 3. The neutralization activity of tetDENV C-LPs was higher than that of tetCPV-LPs for D1-SRIP, while tetCPV-LPs were higher than that of tetDENV C-LPs for D2- and D4-SRIP. These results suggest that homologous tetDENV C-LPs and heterologous tetCPV-LPs can be suitable vaccine candidates for further evaluation. This result is the first report to display a tetEDIII on the surface of the DENV C-LPs and the CPV-LPs by in vitro bioconjugation.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":"2003-2013"},"PeriodicalIF":5.5,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Redox-Responsive Cleavable Polymeric Brush Coated Magnetic Nanoparticles: Fabrication and Post-Polymerization Modification for Cellular Targeting.

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-03-10 Epub Date: 2025-02-04 DOI: 10.1021/acs.biomac.4c01368

Nora Ejderyan, Yavuz Oz, Rana Sanyal, Amitav Sanyal

{"title":"Redox-Responsive Cleavable Polymeric Brush Coated Magnetic Nanoparticles: Fabrication and Post-Polymerization Modification for Cellular Targeting.","authors":"Nora Ejderyan, Yavuz Oz, Rana Sanyal, Amitav Sanyal","doi":"10.1021/acs.biomac.4c01368","DOIUrl":"10.1021/acs.biomac.4c01368","url":null,"abstract":"Polymer brush-coated magnetic nanoparticles find applications in areas from diagnostics to drug delivery. Generally, the brushes are irreversibly tethered onto the nanoparticle surface through robust chemical linkages to withstand diverse environments. The ability to trigger the release of the polymer brushes from the nanoparticle surface once they reach the intracellular environment would be a useful attribute. In this study, we report polymer brushes that undergo release from the nanoparticle surface in a redox-responsive fashion. Furthermore, cleaving the polymer brush also enables precise determination of their molecular weight. Also, we show that fluorescently labeled polymer brushes undergo chain-end functionalization using maleimide-containing dye and peptides. Installing integrin-targeting peptides onto the surface enhances their cellular internalization. One could envision that the redox-responsive polymer brush-coated magnetic nanoparticles disclosed here would be an attractive platform for applications where intracellular cleavage of polymeric chains would enhance their performance.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":"1555-1570"},"PeriodicalIF":5.5,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143187562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Triphenylphosphine-Initiated Ring-Opening Polymerization for α-Helical Polypeptides and Application in Constructing Artificial Ion Channels. 三苯基膦引发的α-螺旋多肽开环聚合及其在人工离子通道构建中的应用

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-03-10 Epub Date: 2025-02-25 DOI: 10.1021/acs.biomac.4c01716

Zexin Yan, Tianlong Li, Jiawei Liu, Juejiao Fan, Shinan Ma, Li Zhao, Chunyan Bao

{"title":"Triphenylphosphine-Initiated Ring-Opening Polymerization for α-Helical Polypeptides and Application in Constructing Artificial Ion Channels.","authors":"Zexin Yan, Tianlong Li, Jiawei Liu, Juejiao Fan, Shinan Ma, Li Zhao, Chunyan Bao","doi":"10.1021/acs.biomac.4c01716","DOIUrl":"10.1021/acs.biomac.4c01716","url":null,"abstract":"In this study, we have screened out an effective triphenylphosphine-derived initiator (2-TMOPP) for efficient ring-opening polymerization of α-amino acid N-carboxyanhydrides (NCA ROP) and demonstrated the potential of the prepared helical polypeptide as an efficient ion channel. By optimizing polymerization conditions, 2-TMOPP exhibited precise control over the molecular weight and polydispersity index for the prepared polypeptides, the universality for different NCA monomers, and the ability to form α-helical secondary structures. By further incorporating ion binding groups and regulating the molecular length, α-helical polypeptide PLCE was capable of inserting into lipid bilayers and possessing the function of ion transport (H+/K+ antiport) via a channel mechanism (EC50 = 12.75 ± 1.58 μg mL-1). PLCE also showed anticancer activity toward HeLa cells, with an IC50 value of approximately 69.67 ± 1.20 μg mL-1 after 20 h coculture, showing the possibility for future practical application in biomedical fields.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":"1904-1912"},"PeriodicalIF":5.5,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transforming Poly(butylene adipate-co-terephthalate) into Protocatechuic Acid via Enzymatic Degradation and Whole-Cell Catalysis. 通过酶降解和全细胞催化转化聚己二酸丁二酯为原儿茶酸。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-03-10 Epub Date: 2025-02-13 DOI: 10.1021/acs.biomac.4c01490

Yunhan Li, Can Su, Fanghui Hu, Jiahuan Ling, Zhidong Zhang, Lihui Zhang

{"title":"Transforming Poly(butylene adipate-co-terephthalate) into Protocatechuic Acid via Enzymatic Degradation and Whole-Cell Catalysis.","authors":"Yunhan Li, Can Su, Fanghui Hu, Jiahuan Ling, Zhidong Zhang, Lihui Zhang","doi":"10.1021/acs.biomac.4c01490","DOIUrl":"10.1021/acs.biomac.4c01490","url":null,"abstract":"Poly(butylene adipate-co-terephthalate) (PBAT) is a biodegradable plastic with increasing applications that degrades rapidly in composting, but its sole degradation limits residual value utilization. Therefore, new strategies for converting PBAT to valuable products are needed. In this study, enzymatic degradation was applied to hydrolyze PBAT, and an engineered Escherichia coli strain was constructed and utilized as a whole-cell catalyst to synthesize protocatechuic acid (PCA), a valuable platform compound, from degradation products. By fine-tuning the overexpression of four enzymes in the biosynthetic pathway and optimizing catalytic conditions, the titer of PCA was increased 5.07-fold to 5.28 ± 0.05 g/L, achieving a 15.45 ± 1.04 wt % conversion rate from pure PBAT particles. Furthermore, the strategy was applicable to commercial PBAT/poly(lactic acid) (PLA), achieving a maximum conversion rate of 25.05 ± 1.21 wt %. Thus, a novel strategy to utilize the PBAT waste was established, which provides a reference for upgrading other macrowaste resources.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":"1701-1708"},"PeriodicalIF":5.5,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyclic Poly(2-methyl-2-oxazoline)-Lipid Conjugates Are Good Alternatives to Poly(ethylene glycol)-Lipids for Lipid Nanoparticle mRNA Formulation. 环状聚（2-甲基-2-恶唑啉）-脂缀合物是用于脂质纳米颗粒mRNA配方的聚（乙二醇）-脂的良好替代品。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-03-10 Epub Date: 2025-02-19 DOI: 10.1021/acs.biomac.4c01587

Bianka Golba, Zifu Zhong, Matteo Romio, Ruben Almey, Dieter Deforce, Maarten Dhaenens, Niek N Sanders, Edmondo M Benetti, Bruno G De Geest

{"title":"Cyclic Poly(2-methyl-2-oxazoline)-Lipid Conjugates Are Good Alternatives to Poly(ethylene glycol)-Lipids for Lipid Nanoparticle mRNA Formulation.","authors":"Bianka Golba, Zifu Zhong, Matteo Romio, Ruben Almey, Dieter Deforce, Maarten Dhaenens, Niek N Sanders, Edmondo M Benetti, Bruno G De Geest","doi":"10.1021/acs.biomac.4c01587","DOIUrl":"10.1021/acs.biomac.4c01587","url":null,"abstract":"Lipid nanoparticles (LNPs) with ionizable cationic lipids have revolutionized RNA drug delivery, playing a key role in the success of mRNA-based therapeutics, such as COVID-19 vaccines. A vital component of these LNPs is the poly(ethylene glycol) (PEG)-lipid conjugate, which enhances colloidal stability but may trigger the production of anti-PEG antibodies, resulting in accelerated blood clearance (ABC) and diminished therapeutic efficacy. In this study, we explored poly(2-methyl-2-oxazoline) (PMOXA) as an alternative stabilizing agent for mRNA LNPs. We synthesized both cyclic and linear PMOXA, conjugated them to dialkyl lipids, and created lipid-polymer amphiphiles. We systematically evaluated how polymer topology influenced the physicochemical properties of LNPs, including in vitro cellular uptake, transfection efficiency, and protein corona formation, and directly compared these properties with those of PEG-stabilized counterparts. In vivo experiments in mice further assessed the biodistribution and protein translation efficiency of these LNPs following intravenous administration. Our results showed that cyclic PMOXA conjugates not only matched but potentially surpassed the performance of PEG-based analogues, highlighting their promise as a superior alternative in mRNA-LNP formulations.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":"1816-1825"},"PeriodicalIF":5.5,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Durability Cellulose-Based Composite Paper with Superior Electromagnetic Interference Shielding.

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-03-10 Epub Date: 2025-02-02 DOI: 10.1021/acs.biomac.4c01750

Xingyu Chen, Fuhao Dong, Sasa Wang, He Liu, Xu Xu

引用次数: 0

Catalase-like Nanozyme-Hybrid Hydrogels Utilizing Endogenous ROS as an Oxygen Source To Synergically Regulate Oxidative Stress and Hypoxia for Enhanced Diabetic Wound Healing. 利用内源性ROS作为氧源协同调节氧化应激和缺氧促进糖尿病伤口愈合的过氧化氢酶样纳米酶-杂交水凝胶

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-03-10 Epub Date: 2025-02-24 DOI: 10.1021/acs.biomac.4c01481

Yehao Chen, Bo Yuan, Zhixuan Yang, Shifeng Yan, Kaixuan Ren, Qingmeng Pi, Yan Liu, Jingbo Yin

{"title":"Catalase-like Nanozyme-Hybrid Hydrogels Utilizing Endogenous ROS as an Oxygen Source To Synergically Regulate Oxidative Stress and Hypoxia for Enhanced Diabetic Wound Healing.","authors":"Yehao Chen, Bo Yuan, Zhixuan Yang, Shifeng Yan, Kaixuan Ren, Qingmeng Pi, Yan Liu, Jingbo Yin","doi":"10.1021/acs.biomac.4c01481","DOIUrl":"10.1021/acs.biomac.4c01481","url":null,"abstract":"High levels of reactive oxygen species (ROS) and hypoxia in diabetic wounds significantly hinder the healing process. In this work, a kind of catalase-like nanozyme-hybrid hydrogel was developed to explore the potential of harnessing endogenous excessive ROS as an oxygen source to synergistically regulate oxidative stress and hypoxia, thereby enhancing diabetic wound healing. The hydrogels exhibited rapid degradation and controlled release of ferrihydrite nanozymes in response to oxidative stress, which continuously catalyzed the decomposition of H2O2 to generate oxygen, effectively scavenging ROS and reducing the risk of local oxygen toxicity. The hydrogels relieved intracellular oxidative stress and the hypoxic microenvironment simultaneously in vitro. The hydrogel dressings effectively inhibited oxidative damage at wound sites, promoted epidermis formation and collagen deposition, and significantly accelerated wound healing in db/db mice. Therefore, the catalase-like nanozyme-hybrid hydrogels represent a promising strategy for diabetic wound dressings, addressing both oxidative stress and hypoxia to improve healing outcomes.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":"1672-1685"},"PeriodicalIF":5.5,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Acetylation Patterns of Xylan on Interactions with Cellulose. 木聚糖乙酰化模式对与纤维素相互作用的影响。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-03-10 Epub Date: 2025-02-27 DOI: 10.1021/acs.biomac.4c01469

Tripti Kundu, Jeremy C Smith, Madhulika Gupta

引用次数: 0