Analytical Chemistry最新文献

{"title":"Dual-Functional Microneedles for In Situ Diagnosis and Biofilm-Targeted Therapy of Diabetic Periodontitis via Biomarker-Responsive Probes and Photothermal NO Nanomotors.","authors":"Jinze Li,Qin Xiang,Guolong Zhao,Kai Lin,Jinkun Huang,Zhuangqiang Gao,Huiting Lu,Haifeng Dong","doi":"10.1021/acs.analchem.5c03316","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c03316","url":null,"abstract":"Chronic periodontitis, a frequent complication of diabetes, is exacerbated by bacterial biofilms that drive progressive periodontal tissue destruction and systemic inflammation. Conventional treatments, utilizing mechanical debridement and systemic antibiotics, often fail to eradicate bacterial biofilms, promote antibiotic resistance, and lack real-time monitoring, leading to suboptimal therapeutic outcomes. Herein, we report a separable bilayer microneedle (MN) patch that enables localized, antibiotic-free, biofilm-targeted therapy and in situ biomarker-based monitoring for the integrated management of chronic periodontitis. Specifically, the swellable backing layer of the patch incorporates interleukin-6 and miRNA-223 responsive fluorescence probes, enabling visual, real-time assessment of periodontal inflammation and aiding in the diagnosis of chronic periodontitis. Simultaneously, the patch features a soluble MN tip embedded with indocyanine-green-modified asymmetrically gold-decorated hollow mesoporous silica nanoparticles, which are loaded with glucose oxidase and l-arginine, forming near-infrared (NIR-) driven nanomotors (JSGAs). Upon NIR irradiation, JSGAs generate local hyperthermia that enhances biofilm penetration and initiates a cascade catalytic reaction to release nitric oxide (NO). In rat models, the system significantly reduced local inflammation and bacterial infiltration while enabling longitudinal monitoring, offering a smart, closed-loop strategy for precise diagnosis and therapy of chronic periodontitis.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"36 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144962730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thioflavin T Is a More General and Less Interfering Probe for Aptamer Binding Assays Than SYBR Green I.","authors":"Yun Shu,Shiyuan Liu,Juewen Liu","doi":"10.1021/acs.analchem.5c03351","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c03351","url":null,"abstract":"Label-free fluorescent binding assays employing DNA staining dyes as probes are widely adopted techniques in the aptamer field. While many dyes have been used, thioflavin T (ThT) did not receive much attention for this purpose until recently, since it has long been perceived primarily as a G-quadruplex staining dye. Based on recent studies, ThT appears to serve as a reliable probe for evaluating the binding of non-G-quadruplex aptamers, and we seek to clarify the underlying mechanisms responsible for the exceptional performance of ThT. We investigated three non-G-quadruplex aptamers targeting adenosine, kanamycin, and Tris(hydroxymethyl)aminomethane (Tris). Consistent Kd values and fluorescence reductions were observed in the presence of ThT regardless of the aptamer-to-dye ratio. In contrast, SYBR Green I (SGI) exhibited responses with apparent Kd values that were far away from the true Kd in most cases. Additional assays demonstrated that ThT exhibits a weaker affinity for aptamers and random DNA sequences compared to SGI. In addition, ThT has weaker affinities to double-stranded DNA than to single-stranded DNA, which is opposite to that for SGI. Lastly, testing the T30695 DNA binding with Pb2+ confirmed that ThT can influence the binding of G-quadruplex aptamers. These findings explain ThT's reliability for probing non-G-quadruplex aptamers.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"66 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144962734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tetrahedral DNA Framework-Engineered Magnetic Nanozyme for Visual Biomarker Sensing via Alkaline Phosphatase-Triggered Signal Switching.","authors":"Mengde Zhao,Jiahui Zhang,Lihua Wang,Na Lu","doi":"10.1021/acs.analchem.5c02300","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c02300","url":null,"abstract":"Point-of-care (POC) detection of prostate-specific antigen (PSA) is critical for the early screening and monitoring of prostate cancer (PCa), which facilitates timely intervention and personalized treatment. However, existing POC platforms suffer from inadequate detection sensitivities, susceptibility to matrix interference, and complex sample pretreatment. To address these issues, we proposed a naked-eye and colorimetric sensing platform based on magnetic nanozyme (Fe3O4@ZIF-67@Pt) integrated with a tetrahedral DNA framework (TDF) and alkaline phosphatase (ALP)-triggered hydrolysis reaction for PSA detection with superior sensing performances. The as-prepared Fe3O4@ZIF-67@Pt nanocomposite synergistically integrates magnetic separation, DNA-conjugated interface engineering, and significantly enhanced peroxidase-like activity, thereby laying the foundation for the construction of high-performance colorimetric biosensors. TDF serves dual functions as a programmable biosensing element, including three vertex anchor ALP reporters for catalytic signal amplification, while the remaining vertex is hybridized with a PSA-specific aptamer to drive competitive target recognition. With the addition of PSA, it preferentially bound to the aptamer, creating a competitive reaction and leading to the release of TDF conjugated to the surface of the magnetic nanozyme. When the ALP-mediated hydrolysis reaction was introduced, the released TDF limited the generation of ascorbic acid (AA) to produce a colorimetric response, resulting in a \"signal-on\" colorimetric assay. The designed platform allowed for the naked-eye and colorimetric detection of PSA in the range of 0.1-1000 ng/mL, with a detection limit as low as 36 pg/mL, while exhibiting excellent selectivity. Additionally, it achieved a high accuracy and satisfactory reliability for PSA detection in human serum samples as well as successfully distinguished prostate cancer patients from healthy individuals. This work holds great potential for application in the development of a reliable POC platform for biomarker detection.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"20 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144962751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dendritic Mesoporous SiO2-Based SERS-Nanozyme-Mediated Immunochromatographic Detection of Foodborne Viruses with Enhanced Sensitivity across Multiple Scenarios.","authors":"Jiaxuan Li,Qing Yu,Zhengkang Li,Zishan Xu,Bingjie Wang,Ting Wu,Guanghua Li,Chongwen Wang,Bing Gu","doi":"10.1021/acs.analchem.5c03047","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c03047","url":null,"abstract":"Detecting low-concentration foodborne viruses in complex samples has long posed a great challenge. Here, we propose a colorimetric enhancement-surface-enhanced Raman scattering (SERS) quantitative dual-mode immunochromatographic assay (ICA), characterized by high flexibility, sensitivity, and stability, which can rapidly and accurately detect viruses in various environments, including field, home, and clinical laboratory settings. A multifunctional SERS nanozyme tag (DSAIA) is customized using dendritic mesoporous SiO2 as the core, which is densely loaded with AuIr catalytic particles and coated with a layer of highly active 35 nm Au nanoparticles on the exterior, thereby simultaneously achieving monodispersity, strong peroxidase activity, and a high density of efficient SERS hotspots. By incorporating antibody-modified DSAIA into the ICA platform, the established technology facilitates rapid dual-mode detection of two significant foodborne viruses, norovirus (NoV) and adenovirus (AdV). In the nanozyme catalytic mode (visual recognition), the detection limits for NoV and AdV reached 0.001 ng/mL and 102 PFU/mL, respectively; in the SERS mode (instrumental analysis), the technology enables precise quantitative analysis and further lowers the detection limits to 0.00014 ng/mL (NoV) and 11 PFU/mL (AdV). Furthermore, by testing 92 clinical stool samples, the practicality of the proposed assay was confirmed, underscoring its immense potential for real-time virus detection.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"15 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144962729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Near Infrared-Responsive Xanthene Probes for Dual-Mode Cancer Treatment and Bioimaging.","authors":"Junfeng Zhang,Lingyu Jin,Bin Xiao,Dongeun Kim,Siwei Hua,Yangmei Hua,Yangjie Liu,Xuefei Huang,Junfeng Kou,Bo Liu,Xianghua Wu,Xiaolin Zhong,Amit Sharma,Yuda Zhu,Wen Xiu Ren,Jong Seung Kim","doi":"10.1021/acs.analchem.5c02888","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c02888","url":null,"abstract":"The incidence of cancer has escalated at a distressing pace, creating an urgent demand for advancements and breakthroughs in medical interventions. Amidst the multitude of approaches for diagnosing and managing cancer, fluorescence imaging, photodynamic therapy, and photothermal therapy have emerged as key approaches due to their unique advantages. Herein, two new xanthene-based probes, namely, JB-1 and JB-2, with D-π-A conjugated structures, are reported. JB-1 and JB-2 each feature electron-donating and electron-accepting groups attached to tetraphenylethene or triphenylamine conjugate skeletons, respectively. The restriction of intramolecular rotation in JB-1 results in an absorption wavelength greater than 638 nm and strong emission at the first near-infrared window at 750 nm, with a 117 nm red-shift compared to ICG. In contrast, JB-2 exhibits an emission peak at 971 nm in the second near-infrared region, with an absorption beyond 691 nm. JB-2 also features a fluorescent shoulder peak at 1204 nm, offering a superior resolution for in vivo bioimaging. Furthermore, JB-2 demonstrates excellent photothermal and photodynamic effects for both in vitro and in vivo cancer therapy. This study highlights the potential for designing multifunctional xanthene dyes for dual-mode cancer treatment and bioimaging.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"84 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144962733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome Analysis of Tear-Derived Extracellular Vesicles as Biomarkers for Diabetic Retinopathy","authors":"Jiaming Chen, Zhaowei Zhang, Xiaoqin Hong, Jingjing Kang, Wanchao Zhang, Rui Du, Jia Xu, Zhaofeng Ding, Chang Li, Dingbin Liu","doi":"10.1021/acs.analchem.5c04102","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c04102","url":null,"abstract":"Diabetic retinopathy (DR) represents the predominant etiology of visual impairment and blindness on a global scale. At present, there is still a dearth of robust biomarkers for DR, which substantially limits its diagnosis, particularly at an early stage. Tears, owing to their inherent proximity to the ocular microenvironment, offer a unique advantage as a source of potential biomarkers for retinal pathologies. Specifically, tear-derived extracellular vesicles (tEVs) have emerged as promising candidates for this purpose, given their ability to reflect the physiological and pathological states of the retina. In this study, we conducted a comprehensive transcriptomic analysis of tEVs and identified a panel of tEV-mRNAs including ODF2L, TSR1, TONSL, and FUT6 as potential biomarkers for the diagnosis of DR. We validated this finding by using 119 tear samples from healthy controls and individuals with varying degrees of retinopathy. Considering the noninvasive and straightforward nature of tear fluid collection, the identified tEV-mRNAs hold significant promise for facilitating the early, noninvasive diagnosis and subsequent management of DR.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"33 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144987519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Refractive Index of Benchmark Polystyrene Nanoplastics by Optical Modeling of UV–Vis Spectra","authors":"Mattia Andrini, Stefania Federici, Luca Gavioli","doi":"10.1021/acs.analchem.5c00168","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00168","url":null,"abstract":"Optical recognition and identification of nanoplastics such as polystyrene nanobeads (PSbs), a widely used polymer and an actual source of environmental pollution, is a challenging task relying on knowledge of the PSbs’ refractive index (RI) and its relation to the PSbs’ morphology. This is, however, lacking for PSbs’ sizes lower than 1 μm. Here, we bridge this gap by measuring UV–vis spectra of PSbs deposited on a sapphire substrate via spin coating and by connecting the experimental data to the RI, PSbs’ morphology, and optical transitions through a new optical model based on the Mie theory. Specifically, the new model allows us to fit the total and diffuse light components considering the particles’ size distribution, the superficial density, and the substrate effects, thus correlating the PSbs’ wavelength-dependent RI features to the experimentally validated specific sample morphology. Two critical model assumptions, i.e., Mie theory and substrate effects, are also discussed employing discrete dipole approximation simulations. Moreover, we identify the optically allowed molecular electronic transitions in nanometric PSbs as potential fingerprints for nanoparticle characterization in more complex matrixes.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"33 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144987483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Coupling of Antenna Effect and Schottky Junction in Tb-Doped Covalent Organic Framework for Enhanced Electrochemiluminescence Sening of Isobutyryl Fentanyl.","authors":"Bingxue Fan,Ding Jiang,Yuan Ni,Zhibang Hu,Zheng Sun,Haibo Li,Wenchang Wang,Zhidong Chen","doi":"10.1021/acs.analchem.5c03193","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c03193","url":null,"abstract":"Rational design of both mechanistic pathways and material compositions is essential to advance COF-based electrochemiluminescence (ECL) systems. In this study, aggregation-induced emission covalent organic framework (AIE-COF) nanoprobes with excellent ECL performance were developed based on Tb3+-functionalized covalent organic framework (Tb@A-COF). The Tb@A-COF system demonstrates enhanced ECL performance through synergistic integration of three complementary mechanisms: (1) (4',4',4',4'-(1,2-ethenediylidene)tetrakis [1,1'-biphenyl]-4-carboxaldehyde (ETBC) ligands function as antenna-like sensitizers that amplify luminescence intensity by 14.62-fold via efficient energy transfer to Tb3+ centers; (2) the constructed silver nanowire-based (Ag NWs) Schottky junction effectively suppresses electrode passivation while improving signal stability through optimized interfacial charge transfer kinetics; (3) cobalt oxide nanosheets (CoOOH NSs) enable dynamic signal modulation via precisely regulated quenching effects on the ECL process. This multidimensional optimization strategy achieves coordinated enhancement of both emission intensity and system stability. The synergistic optimization strategy resulted in an ultrasensitive biosensor capable of detecting isobutyryl fentanyl (iBF) with an ultralow detection limit of 8.97 × 10-16 g/L. This study establishes a multiple amplification strategy for rare-earth-COF composites in the field of ECL, which provides both a conceptual framework for improving the performance of ECL in crystalline frameworks and a new perspective for the development of efficient and stable ECL technology.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"21 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144962732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bromine-Functionalized Covalent Organic Frameworks Enhancing the Desorption Efficiency and Enabling Preconcentration of Brominated Pollutants for Mass Spectrometry Detection","authors":"Yong Tian, Ruijie Zhao, Yanfeng Zhang, Shuo Gao, Haonan Liu, Xiaoxuan Han, Ming-Li Chen, Xuwei Chen, Jian-Hua Wang","doi":"10.1021/acs.analchem.5c03853","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c03853","url":null,"abstract":"Covalent organic frameworks (COFs) exhibit exceptional adsorption capacity but suffer from challenging desorption. In this study, a bromine-functionalized magnetic COF composite (Fe₃O₄@COF-Br) was synthesized via bromodimethylsulfonium bromide-mediated bromination of imine-linked COF on Fe₃O₄ nanospheres (Fe₃O₄@COF), where the COF framework was constructed by 1,3,5-tris(4-formylphenyl)benzene and 2,6-diamino-3,5-diethynylpyridine. Compared with pristine Fe₃O₄@COF, Fe₃O₄@COF-Br enhanced desorption efficiencies for brominated contaminants by about 30% while maintaining adsorption capacity. Systematic characterization and DFT simulations revealed reduced host–guest binding strengths in the brominated composite with interaction energies lowered by about 30 kcal mol^–1. Employing Fe₃O₄@COF-Br as the magnetic solid-phase extraction (MSPE) adsorbent coupled with ESI-MS detection, the method achieved detection limits of 1.4–9.5 ng L^–1 for tetrabromobisphenol A (TBBPA) and its derivatives (TBBPA-BHEE, TBBPA-BGE, TBBPA-BME, TBBPA-BAE, TBBPA-BDBPE), with coefficients of determination (R²) > 0.99 and precision (relative standard deviations) ≤ 8.4%. Validated in landfill leachate, soil, lake water, tap water, juice, soy sauce, and urine matrices, the method delivered 75–125% recoveries, demonstrating practicability and accuracy for complex environmental analysis.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"29 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144987500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-Electrode High-Throughput Dual-Color Electrochemiluminescence Array Biosensor: Portable Parallel Screening of Marine Pathogens","authors":"Yuting Du, Yuanzhen Ning, Wenya Zhang, Guifen Jie","doi":"10.1021/acs.analchem.5c03878","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c03878","url":null,"abstract":"To achieve high-throughput rapid monitoring of marine pathogens, this study developed an innovative single-electrode electrochemiluminescence (SEE) array biosensing imaging platform. For the first time, the highly luminous RuSiO₂–Au and Lu@MIL-NH₂ with a strong nanoconfinement effect were integrated onto a single 10-well microelectrode array. By taking advantage of the extremely strong ECL signals, red-blue dual-color ECL imaging detection of Escherichia coli and Staphylococcus aureus was achieved, which can be clearly distinguished by the naked eye on mobile phones. The two-color design significantly enhances the visual resolution and visibility. The core advantage of this platform lies in its high-throughput design: the 10 independent microreaction cells on the ITO electrode support parallel detection of multiple samples, increasing the throughput by 10 times compared to traditional methods and ensuring good consistency between holes. This unique single-electrode multiarray technology greatly improved the detection efficiency. In terms of the driving mechanism, the platform innovatively abandons the traditional three-electrode system and uses the potential difference induced by resistance to drive the ECL reaction, thereby simplifying the electrode structure, reducing costs, and enhancing portability and ease of operation. Moreover, cyclic amplification technology is integrated into the single-electrode biosensor, which greatly improved the detection sensitivity with the detection limit as low as 30.9 CFU/mL (E. coli) and 25.1 CFU/mL (S. aureus). This study not only constructed a portable and low-cost on-site screening scheme for marine pathogens, but also opened a new single-electrode high-throughput dual-color ECL visualization analysis device, which achieved a new breakthrough in ECL efficiency for biological and environmental assays.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"63 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}