Analytical Chemistry最新文献

Analysis of N-Glycans Released from Glycosylated RNAs 糖基化rna释放的n -聚糖分析

IF 7.3 1区化学

Analytical Chemistry Pub Date : 2026-10-20 Epub Date: 2026-09-23 DOI: 10.1021/acs.analchem.6c03851

Khairul Islam Khan, Liqing He, Lexiao Jin, Eugene G. Mueller, Craig J. McClain, Xiang Zhang

{"title":"Analysis of N-Glycans Released from Glycosylated RNAs","authors":"Khairul Islam Khan, Liqing He, Lexiao Jin, Eugene G. Mueller, Craig J. McClain, Xiang Zhang","doi":"10.1021/acs.analchem.6c03851","DOIUrl":"10.1021/acs.analchem.6c03851","url":null,"abstract":"GlycosylatedRNAs (glycoRNAs) are involved in immune response,cellular function, gene regulation, and disease development. We reporta liquid chromatography–mass spectrometry (LC-MS) method toanalyze glycans released from glycoRNAs. The developed method includesextracting RNA from mouse liver using TRIzol, digesting proteins withproteinase K under denaturing conditions, re-extracting RNA usingTRIzol, releasing N-glycans by enzymatic digestion,enriching glycans by solid-phase extraction, and analyzing glycansby LC-MS. The method was optimized to detect low-abundance N-glycans from a purchased mixture of N-glycan standards, and 65 glycans were identified. We then appliedthe method to analyze the glycans released from the glycoRNAs in mouseliver and identified 244 N-glycans from triplicateanalyses. We also detected 40 significantly changed N-glycans in the livers of 32-week-old female and male KvB2 mice.This study establishes a simple and efficient technique for characterizing N-glycans in glycoRNAs and paves the way for future studiesto understand the function of glycoRNAs.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"98 41","pages":"29588–29595"},"PeriodicalIF":7.3,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149019603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasensitive ppt-Level Detection of Perfluorooctanoic Acid (PFOA) via a Hybrid Nanozyme, Fe3O4@MWCNTs, with Enhanced Peroxidase-Like Activity 通过具有增强过氧化物酶样活性的混合纳米酶Fe3O4@MWCNTs超灵敏的pt水平检测全氟辛酸（PFOA）

IF 7.3 1区化学

Analytical Chemistry Pub Date : 2026-10-20 Epub Date: 2026-09-25 DOI: 10.1021/acs.analchem.6c02884

Pyone Pyone Chit, Khin Moe Lwin, Jungon Yu, Jaehan Lee, Younghoon Cho, Sangil Kim, Kyunghoon Kim

{"title":"Ultrasensitive ppt-Level Detection of Perfluorooctanoic Acid (PFOA) via a Hybrid Nanozyme, Fe3O4@MWCNTs, with Enhanced Peroxidase-Like Activity","authors":"Pyone Pyone Chit, Khin Moe Lwin, Jungon Yu, Jaehan Lee, Younghoon Cho, Sangil Kim, Kyunghoon Kim","doi":"10.1021/acs.analchem.6c02884","DOIUrl":"10.1021/acs.analchem.6c02884","url":null,"abstract":"Increasing concerns over perfluorooctanoicacid (PFOA),a representativeper- and polyfluoroalkyl substance (PFAS) often referred to as “foreverchemicals”, underscore the urgent need for simple, rapid, andsensitive detection strategies for water monitoring. Herein, we reportan ultrasensitive colorimetric sensing platform for monitoring tracelevels of PFOA in environmental water using a highly efficient hybridnanozyme, Fe3O4@MWCNTs. UV–vis absorbanceanalysis confirmed that the nanozyme composite effectively catalyzesthe oxidation of chromogenic substrates, such as 3,3′,5,5′-tetramethylbenzidine(TMB), exhibiting a characteristic blue color in the presence of hydrogenperoxide (H2O2). Upon exposure to PFOA, theactive sites on the nanozyme interface were blocked by PFOA, suppressingTMB oxidation and resulting in a clear “Turn-off” opticalresponse. This sensing platform enabled quantitative detection ofultralow concentrations of PFOA, ranging from 10 to 1,000 ng/L, witha limit of detection (LOD) of 3.12 ng/L. This work provides a cost-effective,rapid, and practical approach for monitoring environmentally relevantPFOA concentrations in water.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"98 41","pages":"29515–29528"},"PeriodicalIF":7.3,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149049913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine-Learning-Driven Dual-Mode Immunoassay Based on Nanobody and Natural Fluorescent Probe for Ultrasensitive Quantification of Pseudomonas aeruginosa 基于纳米体和天然荧光探针的机器学习驱动双模式免疫分析用于铜绿假单胞菌的超灵敏定量

IF 7.3 1区化学

Analytical Chemistry Pub Date : 2026-10-20 Epub Date: 2026-10-06 DOI: 10.1021/acs.analchem.6c04879

Mengnan Wang, Guangsheng Ge, Xueyi Fan, Kai Zheng, Yuxin Miao, Qing Yao, Min Ma, Jianlong Wang, Yanru Wang

{"title":"Machine-Learning-Driven Dual-Mode Immunoassay Based on Nanobody and Natural Fluorescent Probe for Ultrasensitive Quantification of Pseudomonas aeruginosa","authors":"Mengnan Wang, Guangsheng Ge, Xueyi Fan, Kai Zheng, Yuxin Miao, Qing Yao, Min Ma, Jianlong Wang, Yanru Wang","doi":"10.1021/acs.analchem.6c04879","DOIUrl":"10.1021/acs.analchem.6c04879","url":null,"abstract":"Pseudomonas aeruginosa is a Gram-negativeopportunisticbacterial pathogen posing severe threat to environmental safety andhuman health. To achieve rapid and accurate detection, we developeda nanobody-based colorimetric-fluorescent dual-mode sandwich immunosensorusing natural fluorescent probe and further integrated machine learningto enhance the signal readout and improve detection sensitivity. Here,specific nanobodies toward P. aeruginosa were successfullydeveloped for the first time by biopanning against an immune phage-displayednanobody library. Natural fluorescent quinine, which possesses intrinsicfluorescence and undergoes fluorescence quenching via an internalquenching effect, was employed as the fluorescent probe. With thebaseline LODs obtained by direct fitting of the raw optical signals(9.005 × 105 CFU/mL for colorimetry and 3.02 ×103 CFU/mL for fluorescence), an XGBoost model was introducedand further reduces the LOD to 3.269 × 103 CFU/mLand 3.96 × 102 CFU/mL, achieving a 275-fold and 7.6-foldimprovement in sensitivity, respectively. Furthermore, following abrief pre-enrichment, this method enables detection of P. aeruginosa at concentrations as low as 10 CFU/mL. Recoveriesin water samples ranged from 81.47% to 119.47%, with a coefficientof variation below 8.44%, showing good agreement with machine learningpredictions (R2 = 0.957). No cross-reactivitywas observed with closely related or heterologous bacteria. This methodprovides a technical approach for the precise quantitative monitoringof P. aeruginosa in complex environments.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"98 41","pages":"29700–29712"},"PeriodicalIF":7.3,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149091909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Integrating Sphere Based Method for Quantifying RNA Encapsulation Efficiency in Lipid Nanoparticles without Lysis 基于积分球的非裂解脂质纳米粒RNA包封效率定量方法

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2026-10-20 DOI: 10.1021/acs.analchem.6c04173

Aida López Espinar,Eric C. Le Ru,Kirsty Smith,Brendan L. Darby

{"title":"An Integrating Sphere Based Method for Quantifying RNA Encapsulation Efficiency in Lipid Nanoparticles without Lysis","authors":"Aida López Espinar,Eric C. Le Ru,Kirsty Smith,Brendan L. Darby","doi":"10.1021/acs.analchem.6c04173","DOIUrl":"https://doi.org/10.1021/acs.analchem.6c04173","url":null,"abstract":"Encapsulation efficiency (EE) is a critical quality attribute of lipid nanoparticle (LNP)-RNA formulations, determining the fraction of active RNA payload available for delivery and influencing therapeutic potency, dose accuracy, and product consistency. Conventional fluorescence-based EE assays, such as RiboGreen (RG), require detergent-mediated LNP lysis, which can introduce systematic bias arising from incomplete particle disruption and additional sample handling steps. Here, we report a lysis-free method for direct EE determination using Scatter-Free Absorption Spectroscopy (SFAS) within an integrating sphere. In this approach, RG fluorescence appears as a quantitative negative absorbance feature in the SFAS spectrum, enabling direct quantification of free RNA from intact LNP suspensions in a single measurement. Applied to SM-102 LNPs loaded with polyA or mRNA in PBS or Tris/sucrose buffers, the method yielded EE in close agreement with conventional plate-reader measurements. This approach provides a practical, formulation-independent, and potentially more robust strategy for standardized routine EE analysis and LNP quality-control workflows.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"08 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149221711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oligomer-Responsive ThT Derivative for Early Deterioration Evaluation of Insulin Injections 低聚物反应性ThT衍生物用于胰岛素注射早期恶化评估

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2026-10-20 DOI: 10.1021/acs.analchem.6c05164

Yanying Wang,Xianming Li,Zhen Dong,Peng Wu

{"title":"Oligomer-Responsive ThT Derivative for Early Deterioration Evaluation of Insulin Injections","authors":"Yanying Wang,Xianming Li,Zhen Dong,Peng Wu","doi":"10.1021/acs.analchem.6c05164","DOIUrl":"https://doi.org/10.1021/acs.analchem.6c05164","url":null,"abstract":"Insulin, an essential drug for diabetes, is prone to be aggregated due to its inherent amyloidogenicity. Particularly for insulin injections, improper handling or storage (e.g., vibration and heat) can lead to drug deterioration, namely, formation of early oligomers that are highly toxic. Compared to the cloudy amyloid fibrils, the early oligomers are highly soluble and less viscous, thus posing a great challenge for their detection. Thioflavin T (ThT) is the “gold-standard” fluorescent probe for protein aggregation and insulin fibrils but fails in recognition of the early soluble oligomers. Herein, a ThT derivative with a stronger donor unit and a larger molecular volume, namely, ThTPD, was designed with improved viscosity sensitivity and oligomer-binding affinity. In the model of insulin aggregation, ThTPD exhibited an obvious fluorescence response in the very early oligomer stage (∼30 s), whereas ThT only responded to the protofibrils and fibrils (∼30 min). For circular dichroism (CD) spectroscopy that is typically explored for protein aggregation study, a signal emerged until the protofibril formation stage (∼10 min). Real-sample analysis with commercial insulin injections demonstrated that ThTPD could achieve early deterioration evaluation, making it a promising, convenient, and rapid probe for the early deterioration monitoring of insulin injections.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"34 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149221712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-Mimetic Compartmentalization of Natural Enzymes within MOF-Core/COF-Shell Heterostructures as a Versatile Cascade Biosensing Platform MOF-Core/COF-Shell异质结构中天然酶的细胞模拟区隔化作为多功能级联生物传感平台

IF 7.3 1区化学

Analytical Chemistry Pub Date : 2026-10-20 Epub Date: 2026-10-03 DOI: 10.1021/acs.analchem.6c04801

Baozhu Zhao, Zhifei Cheng, Jin Mao, Yanan Zhou, Baocai Xu, Jie Shi, Bo Tang

{"title":"Cell-Mimetic Compartmentalization of Natural Enzymes within MOF-Core/COF-Shell Heterostructures as a Versatile Cascade Biosensing Platform","authors":"Baozhu Zhao, Zhifei Cheng, Jin Mao, Yanan Zhou, Baocai Xu, Jie Shi, Bo Tang","doi":"10.1021/acs.analchem.6c04801","DOIUrl":"10.1021/acs.analchem.6c04801","url":null,"abstract":"Theburgeoning demand for efficient multienzyme cascades necessitatesadvanced scaffolds capable of precise spatial organization; however,constructing heterostructured multilayer frameworks (HMFs) remainsa formidable challenge due to harsh solvothermal conditions and impreciseenzyme localization. Herein, a biocompatible, interface-directed strategyis reported to engineer a cell-mimetic metal–organic framework@covalentorganic framework (MOF@COF) heterostructure as a versatile colorimetricbiosensing platform. Specifically, glucose oxidase immobilizationwithin defect-rich (COOH)2-UiO-66 via competitive coordinationgenerated robust sensing elements. Subsequently, ionic liquid-catalyzedinterfacial nucleation orchestrated the in situ growthof a horseradish peroxidase-encapsulated COF shell, yielding a compartmentalizedarchitecture with hierarchical channels. Mechanistic investigationsand density functional theory (DFT) calculations reveal that the support’sdefect density and interfacial adsorption energy, rather than long-rangecrystallinity, dictate the directional assembly of the COF layer.This heterostructure exhibits enhanced cascade activity and analyticalreliability through spatial proximity and reduced mass transfer resistanceover conventional one-pot or stepwise assemblies, while control studieswith zeolitic imidazolate framework-8 and hydrogen-bonded organicframework counterparts underscore its superiority in proximity-inducedintermediate transfer. Integrating monomer reactivity with topologicalsymmetry offers a predictive blueprint for customizing structurallydiverse HMF libraries. Ultimately, flexible replacement of the coreenzyme enables the functional validation of this platform throughthe colorimetric quantification of glucose and hypoxanthine, offeringa rational blueprint for engineering biointerfaced heterostructuresin cascade biocatalysis.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"98 41","pages":"29657–29675"},"PeriodicalIF":7.3,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149092141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DES-Based Green Synthesis of 2D Fluorescent Molybdenum Disulfide for Highly Sensitive and Selective Detection of Nifedipine 基于des的二维荧光二硫化钼绿色合成用于硝苯地平的高灵敏度和选择性检测

IF 7.3 1区化学

Analytical Chemistry Pub Date : 2026-10-20 Epub Date: 2026-10-01 DOI: 10.1021/acs.analchem.6c02269

Rafee Ullah, Shams Ur Rehman, Adnan Khan, Hui-Fen Wu

{"title":"DES-Based Green Synthesis of 2D Fluorescent Molybdenum Disulfide for Highly Sensitive and Selective Detection of Nifedipine","authors":"Rafee Ullah, Shams Ur Rehman, Adnan Khan, Hui-Fen Wu","doi":"10.1021/acs.analchem.6c02269","DOIUrl":"10.1021/acs.analchem.6c02269","url":null,"abstract":"Highly fluorescent 2D MoS2–x nanosheetsexhibitingintense blue emission were synthesized using a green chemistry approach,utilizing DES as both the reaction medium and a surface passivatingagent. The material was synthesized via a hydrothermal method, followedby ultrasonication-assisted exfoliation to yield ultrathin nanosheetswith enhanced photoluminescence properties. The 2D MoS2–x nanosheets demonstrated strong fluorescence at λem = 420 nm when excited at λex = 330 nm. These nanosheetswere employed as a fluorescence probe for the detection of the calciumchannel blocker drug nifedipine (NIF) via a fluorescence quenchingmechanism. Upon addition of NIF, the fluorescence of the 2D MoS2–x nanosheets was quenched due to electron transferfrom the nanosheets to the electron-deficient NIF molecules, combinedwith the inner filter effect (IFE). The nanosheets enabled highlysensitive and selective detection of NIF, with photoluminescence spectroscopyrevealing a broad linear detection range of 0.6–360 nM (R2 = 0.993) and an exceptionally low limit of detection (LOD)of 0.40 nM. The system showed high selectivity toward NIF over structurallysimilar compounds, drugs, and common metal ions. The sensor was effectivelyapplied for the detection of NIF in urine and Lotus Pond water samples,highlighting its potential for practical pharmacological monitoring.This study presents a sustainable and effective approach for fabricatingDES-assisted 2D nanosheets for trace-level analyte detection.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"98 41","pages":"29445–29458"},"PeriodicalIF":7.3,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149091824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Single Injection to Dual Insights: Integrating EAD and CID Acquisition for Spectral Library Generation and EAD–CID Spectral Matching 从单一注入到双重洞察：光谱库生成和ead - cidd光谱匹配的集成ead和CID采集

IF 7.3 1区化学

Analytical Chemistry Pub Date : 2026-10-20 Epub Date: 2026-10-02 DOI: 10.1021/acs.analchem.6c01204

Lisa Panzenboeck, Christina Brenner, Veronika Fitz, Mingxun Wang, Patrick Mueller, Evelyn Rampler, Yasin El Abiead, Gunda Koellensperger

{"title":"From Single Injection to Dual Insights: Integrating EAD and CID Acquisition for Spectral Library Generation and EAD–CID Spectral Matching","authors":"Lisa Panzenboeck, Christina Brenner, Veronika Fitz, Mingxun Wang, Patrick Mueller, Evelyn Rampler, Yasin El Abiead, Gunda Koellensperger","doi":"10.1021/acs.analchem.6c01204","DOIUrl":"10.1021/acs.analchem.6c01204","url":null,"abstract":"Mass spectral MS/MSlibraries are essential in nontargeted metabolomicsas they facilitate metabolite annotation and provide the basis forin silico annotation tools and fragmentation rules. While significantprogress has been made toward more comprehensive MS/MS libraries,those efforts have focused mainly on collision-induced dissociation(CID)- and higher-energy collisional dissociation (HCD)-based approaches.In this work, we introduce a flow injection-high-resolution targetedMS/MS-based acquisition strategy that acquires electron-activateddissociation (EAD) and CID across a wide range of fragmentation energieswithin a single injection. The workflow supports both nontargetedand targeted analysis, through efficient library generation and MS/MSparameter optimization, respectively. We apply the workflow via aset of 45 steroid standards, known for their important role in humanhealth, disease, and doping, expanding the coverage of public librariesfor both CID and EAD. Finally, we utilize public MS/MS libraries andraw data to reveal that EAD MS/MS spectra can, to some extent, beannotated via existing MS/MS libraries, governed by CID and HCD, andshow that the acquired CID library enables novel annotations in publiclyavailable clinical data, demonstrating its utility for biologicalstudies.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"98 41","pages":"29433–29444"},"PeriodicalIF":7.3,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149092145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultra-High Peak Capacity Exceeding 5 Peaks per Second Enabled by LC×LC-SLIM-qTOF-MS for the Analysis of Complex Samples 每秒超过5个峰的超高峰值容量通过LC×LC-SLIM-qTOF-MS支持复杂样品的分析

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2026-10-20 DOI: 10.1021/acs.analchem.6c03659

Priscilla Nhan,Cedric Thom,Sven W. Meckelmann,Oliver J. Schmitz

{"title":"Ultra-High Peak Capacity Exceeding 5 Peaks per Second Enabled by LC×LC-SLIM-qTOF-MS for the Analysis of Complex Samples","authors":"Priscilla Nhan,Cedric Thom,Sven W. Meckelmann,Oliver J. Schmitz","doi":"10.1021/acs.analchem.6c03659","DOIUrl":"https://doi.org/10.1021/acs.analchem.6c03659","url":null,"abstract":"Comprehensive two-dimensional chromatography substantially enhances separation performance prior to mass spectrometric detection compared to conventional one-dimensional approaches. This results in (i) cleaner and more readily interpretable mass spectra and (ii) reduced ion suppression in atmospheric pressure ionization sources. To further increase separation performance within a reasonable total analysis time, comprehensive LC×LC was coupled to a SLIM-IM-qTOF-MS system for the analysis of complex herbal liqueur samples. To facilitate the processing of multidimensional data, a long modulation time of 4 min was applied. In addition, trap-based modulation was implemented to improve analyte focusing and optimize two-dimensional pre-separation. A peak capacity of LC×LCn = 201 was achieved for the LC×LC separation. Incorporating ion mobility as a third separation dimension further increased the total peak capacity. Total peak capacities of Ptotal(SLIM) = 16,683 and Ptotal(DTIMS) = 8643 were achieved for the SLIM and DTIMS coupling, respectively. The SLIM coupling therefore provided an approximately 1.9-fold higher total peak capacity than the DTIMS coupling, demonstrating its superior separation potential.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"88 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149221671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Cell ICP−MS Quantification of Intracellular Chromium and Cell-Surface TNFR2 for Assessing Cr(III/VI)-Induced Lung Epithelial Injury and COPD-Relevant Responses 单细胞ICP - MS定量细胞内铬和细胞表面TNFR2用于评估Cr（III/VI）诱导的肺上皮损伤和copd相关反应

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2026-10-20 DOI: 10.1021/acs.analchem.6c03017

Xinyi Lu,Beibei Chen,Man He,Bin Hu

{"title":"Single-Cell ICP−MS Quantification of Intracellular Chromium and Cell-Surface TNFR2 for Assessing Cr(III/VI)-Induced Lung Epithelial Injury and COPD-Relevant Responses","authors":"Xinyi Lu,Beibei Chen,Man He,Bin Hu","doi":"10.1021/acs.analchem.6c03017","DOIUrl":"https://doi.org/10.1021/acs.analchem.6c03017","url":null,"abstract":"An integrated platform combining droplet microfluidics, lanthanide labeling, and inductively coupled plasma mass spectrometry (ICP−MS) was proposed to quantify intracellular heavy metal and cell-surface protein at single-cell resolution. The workflow performs parallel measurements on homologous single-cell subsets to establish statistical correlations between metal accumulation and functional protein responses. Using tumor necrosis factor receptor 2 (TNFR2) as a model protein, we investigated the effects of atmospheric particulate matter-associated heavy metals on human lung epithelial cells (BEAS-2B). Several heavy metals trigger TNFR2 upregulation, with Cr(VI) exerting the most pronounced effect. The Cr(VI)-induced increase in TNFR2 expression was associated with elevated TNF-α release, higher intracellular ROS, and reduced SOD activity but did not correlate directly with intracellular Cr accumulation, likely due to distinct cellular uptake mechanisms between Cr(III) and Cr(VI). High-concentration Cr(III) exposure, however, led to intracellular Cr enrichment and provoked inflammatory responses. Integration of differential gene expression, TNF-α release, and TNFR2 expression support TNFR2 as a potential indicator of metal-induced oxidative stress, epithelial injury, and COPD-relevant inflammatory responses. This single-cell dual-parameter approach provides a new strategy for linking metal uptake to functional protein responses in environmental toxicology.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"11 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149221714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0