Analytical Chemistry最新文献

Construction of Flexible Electrochemical Sensing Interfaces Based on Paper/PEDOT:PSS for the Detection of Cystatin C. 基于Paper/PEDOT:PSS柔性电化学传感界面的构建检测胱抑素C

IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-07-04 DOI: 10.1021/acs.analchem.5c01627

Yang Li, Yixuan Bian, Regan McMath, James Davis, Pagona Papakonstantinou, Yuanhua Shao, Meixian Li

{"title":"Construction of Flexible Electrochemical Sensing Interfaces Based on Paper/PEDOT:PSS for the Detection of Cystatin C.","authors":"Yang Li, Yixuan Bian, Regan McMath, James Davis, Pagona Papakonstantinou, Yuanhua Shao, Meixian Li","doi":"10.1021/acs.analchem.5c01627","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01627","url":null,"abstract":"Chronic kidney disease (CKD) is a global public health issue, with high prevalence and mortality. As cystatin C is a biomarker for the early diagnosis of CKD, there is an urgent need to develop a simple and inexpensive method for the detection of Cys C in human plasma. Herein, a sensitive method with flexible electrochemical aptamer-based (E-AB) sensing interfaces has been established using poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate)-treated paper-based electrodes. Introduction of MoS2 quantum dots increased the specific surface area and realized effective immobilization of the thiolated Cys C aptamer (Cys C Apt) modified with methylene blue through the formation of disulfide bonds. The E-AB sensing interfaces were successfully applied to the quantification of Cys C in human venous whole blood and plasma samples based on the binding-induced conformational change mechanism of Cys C Apt by employing square wave voltammetry. The flexible E-AB sensing interfaces show significant potential to be developed into wearable patches for in vivo detection of Cys C, which will pave the way for early diagnosis and long-term monitoring of CKD.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":" ","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proposed Method for Label-Free Separation and Infrared Spectroscopy of Carbonyl-Containing Micro- and Nanoparticles Using Mid-Infrared Optical Force. 基于中红外光力的含羰基微粒子和纳米粒子无标记分离和红外光谱分析方法。

IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-07-04 DOI: 10.1021/acs.analchem.5c02185

Y Albert Darmawan, Taiki Yanagishima, Takao Fuji, Tetsuhiro Kudo

{"title":"Proposed Method for Label-Free Separation and Infrared Spectroscopy of Carbonyl-Containing Micro- and Nanoparticles Using Mid-Infrared Optical Force.","authors":"Y Albert Darmawan, Taiki Yanagishima, Takao Fuji, Tetsuhiro Kudo","doi":"10.1021/acs.analchem.5c02185","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c02185","url":null,"abstract":"Separation and spectroscopy are essential and complementary techniques in molecular analysis, including gas/liquid chromatography, electrophoresis, flow cytometry, and vibrational spectroscopies, where independent modalities are required for separation and spectral characterization. In many cases, separated materials require spectroscopic analysis, whereas characterized components in a mixture may need further separation. Here, we present a mid-infrared optical force technique in which spectroscopic differences alone can be directly utilized to separate materials based on their molecular species and structures without labeling. In particular, we demonstrate an optical manipulation of micro- and nanospheres via a tunable mid-infrared laser, where their velocity, induced by optical force, at different wavenumbers closely match the Fourier-transform infrared spectra of the constituent material. The mid-infrared laser covers the spectral range of the vibrational mode of carbonyl bonds in the particles: we successfully demonstrate the selective manipulation of PMMA (poly(methyl methacrylate)) and TPM (3-(trimethoxysilyl)propyl methacrylate), which contain the same carbonyl bonds but in different surrounding environments. The experimental results agree with optical force calculations based on the finite-difference time-domain simulation. This constitutes the first direct evidence that the velocity is proportional to infrared absorbance at different wavenumbers, enabling precise reconstruction of infrared absorbance spectra from measured velocities. We believe that the proposed method enables a versatile particle separation and characterization across a wide range of materials, e.g., cells, nucleic acids, viruses, proteins, and potentially down to molecules, as the mid-infrared region is home to the molecular vibrational modes in a vast array of compounds.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":" ","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Why Did Not ECD Dissociate between Glycans in di-N-Glycosylated Peptides? Survey and Solution. 为什么ECD不能在二n -糖基化肽的聚糖之间解离？调查和解决方案。

IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-07-04 DOI: 10.1021/acs.analchem.5c01461

Takashi Baba, J C Yves Le Blanc, Stanislav Beloborodov, Pavel Ryumin

{"title":"Why Did Not ECD Dissociate between Glycans in di-N-Glycosylated Peptides? Survey and Solution.","authors":"Takashi Baba, J C Yves Le Blanc, Stanislav Beloborodov, Pavel Ryumin","doi":"10.1021/acs.analchem.5c01461","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01461","url":null,"abstract":"In previously reported electron capture dissociation (ECD) mass spectrometry of N-linked glycopeptides, ECD did not provide efficient backbone fragmentation between the two N-glycans in a single peptide, which did not allow the assignment of a correct sugar composition in each glycan. In this study, we found hydrogen bonds and proton-bound interactions between two N-glycans, so the backbone fragments between the two N-glycans were not observed, although the backbone was cleaved. Such behavior is typical for precursor ions produced by conventional electrospray ionization. Supercharging reagent 3-nitrobenzyl alcohol increases the precursor charge state, which leads to the protonation of each N-glycan, and the resulting Coulombic repulsive force is sufficient to quench the noncovalent binding. Highly charged precursors produced using the supercharged reagents yield intense c' and z· fragments between the N-glycans in ECD analysis. Computational molecular structure calculations and differential mobility spectrometry validated the model of glycan formations in precursors.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":" ","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Framework for Accurate Single-Molecule Spectroscopic Imaging Analyses Using Monte Carlo Simulation and Deep Learning. 使用蒙特卡罗模拟和深度学习的精确单分子光谱成像分析框架。

IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-07-04 DOI: 10.1021/acs.analchem.5c01486

Hongjing Mao, Yunshu Liu, Obblivignes KanchanadeviVenkataraman, Md Abul Shahid, Caroline Laplante, Dongkuan Xu, Ki-Hee Song, Yang Zhang

{"title":"Framework for Accurate Single-Molecule Spectroscopic Imaging Analyses Using Monte Carlo Simulation and Deep Learning.","authors":"Hongjing Mao, Yunshu Liu, Obblivignes KanchanadeviVenkataraman, Md Abul Shahid, Caroline Laplante, Dongkuan Xu, Ki-Hee Song, Yang Zhang","doi":"10.1021/acs.analchem.5c01486","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01486","url":null,"abstract":"Accurate single-molecule spectral imaging denoising and analysis are essential for advancing high-throughput single-molecule spectroscopy and spectrally resolved super-resolution microscopy. However, a standardized framework for guiding the accurate analysis of single-molecule spectral data remains unavailable. To address this, we developed an analysis framework that generates ground truth (GT) single-molecule spectral imaging data using Monte Carlo simulations, enabling the first supervised learning-based imaging denoising method (referred to as SpecUNet) for single-molecule spectral images. Within this framework, we established eight comprehensive evaluation metrics to systematically compare the performance of SpecUNet against existing imaging analytics using synthetic GT data. We further validated SpecUNet's performance experimentally and demonstrated its capability in accurately characterizing the single-molecule fluorescence spectral heterogeneity of Janelia Fluors. Additionally, we explored its ability to decode the spectral responses of the polarity-sensitive probe Nile Red under varying nanoscale chemical polarities and heterogeneities.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":" ","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environment-Friendly MnO2 Quantum Dots with Highly Efficient Electrochemiluminescence for Ultrasensitive Assay of Liver Cancer Markers. 高效电化学发光的环保型二氧化锰量子点用于肝癌标志物的超敏检测。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-07-03 DOI: 10.1021/acs.analchem.5c02733

Ying Zeng,Yu-Zhuo Guo,Jia-Li Liu,Ruo Yuan,Ya-Qin Chai

{"title":"Environment-Friendly MnO2 Quantum Dots with Highly Efficient Electrochemiluminescence for Ultrasensitive Assay of Liver Cancer Markers.","authors":"Ying Zeng,Yu-Zhuo Guo,Jia-Li Liu,Ruo Yuan,Ya-Qin Chai","doi":"10.1021/acs.analchem.5c02733","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c02733","url":null,"abstract":"In this work, environment-friendly MnO2 quantum dots (MnO2 QDs) with high efficiency were first proposed as electrochemiluminescence (ECL) emitters to construct a biosensor for ultrasensitive detection of microRNA-222 (miRNA-222) related to liver cancer markers. Compared with reported QDs containing heavy metal elements, MnO2 QDs not only exhibited remarkable ECL efficiency but also displayed the advantage of good biocompatibility, showing promising application prospects in the biosensor field. Impressively, silver nanoparticles (Ag NPs) as coreaction accelerators could promote the conversion of the coreactant S2O82- into abundant SO4•- to produce more MnO2 QDs* to significantly enhance the ECL efficiency of MnO2 QDs. Furthermore, an efficient DNAzyme-Induced Self-Feedback DNA Amplification (DISFA) strategy was developed by employing DNAzyme to specifically cleave double-loop hairpins and release target analogues, achieving the conversion of trace miRNA-222 into abundant output DNA and improving the amplification efficiency and detection sensitivity. The ECL biosensor composed of a MnO2 QDs/S2O82-/Ag NP ternary system and DISFA strategy displayed an excellent linear response of miRNA-222 from 100 aM to 100 pM with a low detection limit of 12.90 aM (S/N = 3) and was successfully implemented for the analysis of miRNA-222 in cancer cell (MHCC-97L and HeLa) lysates. This study proposed transition metal oxide QDs (TMO QDs) with good biocompatibility as efficient and stable ECL emitters for the sensitive detection of biomarkers, demonstrating immense potential for disease diagnosis.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"20 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144547713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apoferritin Nanoparticle-Based Mass Tags: A Novel Metal Tagging Strategy for Mass Cytometry. 载铁蛋白纳米颗粒为基础的质量标签：一种新的金属标记策略，用于质量细胞术。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-07-03 DOI: 10.1021/acs.analchem.5c01918

Jinhui Liu,Weiliang Liu,Zhian Hu,Zhi Xing,Gongwei Sun,Meng Wang,Sichun Zhang,Xinrong Zhang

{"title":"Apoferritin Nanoparticle-Based Mass Tags: A Novel Metal Tagging Strategy for Mass Cytometry.","authors":"Jinhui Liu,Weiliang Liu,Zhian Hu,Zhi Xing,Gongwei Sun,Meng Wang,Sichun Zhang,Xinrong Zhang","doi":"10.1021/acs.analchem.5c01918","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01918","url":null,"abstract":"Mass cytometry (MC), considered the next generation of flow cytometry (FC), uses antibodies tagged with metal isotopes instead of fluorescent molecules for higher-dimensional single cell biomarker assays and can measure more than 50 parameters simultaneously on individual cells. Despite its powerful analytical performance, MC also has limitations in sensitivity and isotope channels using current mass tags. Herein, a new metal tagging strategy was developed to prepare MC mass tags based on apoferritin nanoparticles (AFNPs). For the preparation of AFNPs, rare-earth metals and phosphate ions were sequentially introduced into the cavity of apoferritin by a passive diffusion method to form a metal-phosphate core inside the apoferritin nanocages. The N-hydroxysuccinimide polyethylene glycol (MW = 1000, n = 22) maleimide (NHS-PEG22-MAL) linker was used to link the AFNPs and antibodies to prepare Ab-NP conjugates. The AFNPs have low nonspecific binding to cells, thus resulting in a low background signal for the MC assay. Under optimized conditions, each apoferritin can load an average of 300-800 rare-earth metal atoms, and AFNP tags have more than twice the sensitivity compared with metal-chelating polymer (MCP) tags. Multiparameter assays for the cellular subset analysis of human peripheral blood mononuclear cells (PBMCs) showed good agreement between FC and MC assays by using our AFNP tags. The study presents a new strategy for preparing MC mass tags that are chelator-independent and have multiple staining capabilities, low nonspecific binding, and high sensitivity. The developed AFNP tags are expected to promote the development of MC technology.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"5 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144547795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Antiviral Activity of Lactoferrin in Bovine Milk Toward Fondaparinux Using Ordered Porous Layer Interferometry. 用有序孔层干涉法评价牛奶中乳铁蛋白对Fondaparinux的抗病毒活性。

IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-07-03 DOI: 10.1021/acs.analchem.5c01351

Yu Zhang, Liming Liu, Yizhen Wan, Tianze Wang, Xiaoling Zheng, Bo Zhang, Dongmin Zhao, Huili Wang, Weiping Qian

{"title":"Evaluation of Antiviral Activity of Lactoferrin in Bovine Milk Toward Fondaparinux Using Ordered Porous Layer Interferometry.","authors":"Yu Zhang, Liming Liu, Yizhen Wan, Tianze Wang, Xiaoling Zheng, Bo Zhang, Dongmin Zhao, Huili Wang, Weiping Qian","doi":"10.1021/acs.analchem.5c01351","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01351","url":null,"abstract":"Lactoferrin (LF) is a bioactive molecule that plays a pivotal role in innate immunity and is widely present in dairy products. It has been demonstrated to restrict viral infections by blocking binding sites on heparan sulfate (HS). However, existing analytical methods primarily focus on the LF content rather than its bioactivity, highlighting the need for a dedicated approach to antiviral LF analysis. Developing an appropriate tool for antiviral LF analysis in dairy products is crucial for consumer welfare and product quality control, as the daily intake of LF-rich products offers significant health benefits. However, in one of the primary LF-rich dietary sources, bovine milk, the main limitation of current analysis methods is the lack of an efficient receptor for characterizing antiviral LF. In this study, we developed fondaparinux as a promising receptor for antiviral LF analysis and introduced ordered porous layer interferometry (OPLI) for real-time, in situ monitoring of antiviral LF. Furthermore, we compared the proposed method with the enzyme-linked immunosorbent assay (ELISA) and evaluated antiviral LF content across different milk samples.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":" ","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycoproteomics Analysis to Identify Potential Biomarkers and Investigate CD14+ Monocyte Activation Mechanisms via Exosomal Proteins in Cerebral Infarction Using a Novel Glycogen-Functionalized Nanoprobe. 糖蛋白组学分析鉴定潜在的生物标志物和研究CD14+单核细胞激活机制，通过外泌体蛋白在脑梗死中使用新的糖原功能化纳米探针。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-07-03 DOI: 10.1021/acs.analchem.5c00004

Xiao Tong,Weiwei Chen,Yan Xia,Yanmei Wang,Shuangshuang Liu,Yingli Jia,Jiaxi Wang,Li-Hao Huang

{"title":"Glycoproteomics Analysis to Identify Potential Biomarkers and Investigate CD14+ Monocyte Activation Mechanisms via Exosomal Proteins in Cerebral Infarction Using a Novel Glycogen-Functionalized Nanoprobe.","authors":"Xiao Tong,Weiwei Chen,Yan Xia,Yanmei Wang,Shuangshuang Liu,Yingli Jia,Jiaxi Wang,Li-Hao Huang","doi":"10.1021/acs.analchem.5c00004","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00004","url":null,"abstract":"Cerebral infarction (CI) is a leading cause of disability and mortality, with activated plasma monocytes and altered protein glycosylation identified as critical contributors to its pathology. However, the mechanisms linking peripheral monocyte activation and glycoproteins in CI remain inadequately understood. In this study, we developed a glycogen-functionalized nanoprobe (mSiO2@Fe3O4@Glycogen) to profile plasma N-glycosylated proteins in healthy controls, CI patients, and patients in the rehabilitation phase (CIR). We identified 13 plasma N-glycoproteins with increased expression in CI patients and reduced levels in CIR patients. These N-glycoproteins, enriched in extracellular exosomes and associated with processes such as blood coagulation, fibrin clot formation, and complement activation, were validated as potential biomarkers for distinguishing CI and CIR patients through machine learning models. Flow cytometry analysis of peripheral blood mononuclear cells (PBMCs) revealed a higher proportion of inflammatory CD14+ monocytes and a lower proportion of anti-inflammatory CD16+ monocytes in CI patients compared with CIR patients. To further investigate whether exosomal proteins contribute to peripheral monocyte inflammation and could exacerbate CI pathogenesis, we developed a two-dimensional size-exclusion chromatography (SEC) method to isolate pure plasma exosomes for proteomic analysis. Four specific N-glycoproteins carried by CI-derived exosomes were identified and demonstrated the ability to directly activate human monocytes, potentially amplifying inflammation at injury sites. Our study highlights the role of exosomal N-glycoproteins in CI pathogenesis and positions them as promising diagnostic biomarkers and therapeutic targets for cerebral infarction.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"1 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144547794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Sensitivity TDLAS Sensors Based on Double-Helix United/Split Spot Patterns. 基于双螺旋联合/分裂光斑模式的高灵敏度TDLAS传感器。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-07-03 DOI: 10.1021/acs.analchem.5c03159

Haiyue Sun,Xiaorong Sun,Ying He,Shunda Qiao,Yufei Ma

引用次数: 0

Image Fusion for Improving the Spatial Resolution of LA-ICP-MS Imaging. 提高LA-ICP-MS成像空间分辨率的图像融合。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-07-03 DOI: 10.1021/acs.analchem.5c01925

Teerapong Jantarat,Jeerapat Doungchawee,Xianzhi Zhang,Vincent M Rotello,Richard W Vachet

{"title":"Image Fusion for Improving the Spatial Resolution of LA-ICP-MS Imaging.","authors":"Teerapong Jantarat,Jeerapat Doungchawee,Xianzhi Zhang,Vincent M Rotello,Richard W Vachet","doi":"10.1021/acs.analchem.5c01925","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01925","url":null,"abstract":"Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) imaging has been employed to determine elemental distributions in biological tissues and has proven to be valuable for studying nanomaterials used in drug delivery systems. However, in LA-ICP-MS imaging, there are often trade-offs between achieving higher spatial resolution, maintaining sensitivity, and minimizing acquisition time. Using a larger laser spot size retains sensitivity and allows for faster image acquisition, but the resulting poorer resolution is often insufficient for characterizing the distributions of nanomaterials in functional units of organs, such as the kidney, liver, and spleen. In this work, we describe an approach to enhance the spatial resolution of LA-ICP-MS imaging using image fusion through the computational integration of LA-ICP-MS and optical microscopy images. This approach maintains the highly precise and sensitive elemental distributions inherent to LA-ICP-MS while leveraging the high spatial resolution of optical microscopy to obtain more detailed suborgan maps for both biological metals and nanomaterials. Our approaches enable the construction of LA-ICP-MS images with 5 μm resolution without changes to hardware, operational time, or sensitivity. The resulting enhanced resolution yields new insights into nanomaterial excretion through the liver and the role of the immune system in recognizing gold nanoparticles in the spleen. This fusion approach promises to serve as a valuable tool for advancing nanomaterial-based drug delivery systems.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"150 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144547860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0