Analytical Chemistry最新文献

{"title":"Investigation of Characteristic Volatile Organic Compounds in Esophageal Cancer Organoids by Mass Spectrometry.","authors":"Xiangxue Zheng,Dianlong Ge,Jijuan Zhou,Yue Liu,Yajing Chu,Wenting Liu,Li Ke,Yan Lu,Chaoqun Huang,Chengyin Shen,Yannan Chu","doi":"10.1021/acs.analchem.5c01323","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01323","url":null,"abstract":"Volatilomics, an emerging field in the study of noninvasive biomarkers, holds significant promise for identifying esophageal cancer (EC) through the detection of volatile organic compounds (VOCs). This study investigates common characteristic VOCs of EC cells by analyzing VOCs from three-dimensional organoid and spheroid models as well as two-dimensional monolayer cultures. The VOC profiles were measured by using solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) in an untargeted approach. In the organoid, spheroid, and monolayer culture models, 21, 9, and 8 metabolically differential VOCs were identified between EC and normal esophageal (NE) cells, respectively. Correlation analysis across these three models revealed a shared characteristic VOC of EC: ethyl 2-methylbutyrate. This VOC was markedly increased in EC, demonstrating excellent diagnostic potential with an area under the curve (AUC) ranging from 0.93 to 0.99. Furthermore, transcriptomic data analysis of EC and NE tissues revealed the upregulation of protein degradation and absorption in EC tissues, supporting the hypothesis that ethyl 2-methylbutyrate arises from abnormalities within this metabolic pathway. This study not only supplies potential VOC biomarkers for EC identification but also provides a scientific basis for further elucidating the biochemical mechanisms underlying EC metabolic disturbances, potentially facilitating the gaseous biopsy technique development for EC diagnosis.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"25 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144701198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyrrole-Modified Two-Dimensional Carbon Nitride Nanoparticles Realize Super-Resolution Imaging.","authors":"Yuyang Wu,Ze Zhang,Zhe Wang,Chenhao Yu,Zhipeng Huang,Yichen Gu,Zongjun Li,Shengyan Yin,Guangbin Wang","doi":"10.1021/acs.analchem.5c01681","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01681","url":null,"abstract":"Graphitic carbon nitride (g-C3N4), as an excellent optoelectronic material, has potential applications in bioimaging. However, the original g-C3N4 has an excessively large size and poor water solubility and is difficult to modify, making it unsuitable for direct use in bioimaging. Here, we propose an efficient modification scheme. By using pyrrole as the modifying reagent, we achieve size refinement and solubility improvement, enhance the fluorescence intensity of g-C3N4, and endow it with fluorescence scintillation properties. Subsequently, this modified C3N4 was transformed to water-soluble fluorescent probes through the nanoprecipitation method. Finally, with the help of specific antibodies, we achieved super-resolution imaging of cell microtubule structures using this fluorescent probe with a resolution of up to 180 nm. This research method not only overcomes the difficulties of applying two-dimensional rigid materials to biological applications but also provides a new approach of other two-dimensional materials in small sized and soluble fluorescent probes.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"26 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144701255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating Metabolic Pathways of Ankylosing Spondylitis via Compound Similarity Network-Assisted Metabolomics Analysis.","authors":"Jinxia Hu,Xuean Wang,Hailiang Li,Shengquan Zeng,Bin Yang,Feng Li,Yanan Tang","doi":"10.1021/acs.analchem.5c00449","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00449","url":null,"abstract":"LC-MS-based metabolomics is a powerful tool in analyzing disease molecular mechanisms. Because of its high sensitivity and throughput, LC-MS-based metabolomics usually detects thousands of metabolites. How to find disease-related metabolites and investigate metabolic pathways is critical in metabolomics studies. Conventional statistics-guided data mining looks only for mathematical relations between the detected metabolites and the metadata. It is not enough to unveil biological pathways of metabolites involved in disease progression. Compound similarity network (CSN) is a spectral-independent technique to cluster compounds based on their structural similarities and to investigate potential chemical transformations. Herein, we developed a CSN-assisted metabolic data mining strategy to quantitatively find key metabolites in diseases through structural similarities and explore disease-regulating metabolic pathways based on KEGG and RetroRules metabolic reaction templates. The strategy was used in a metabolomics study of ankylosing spondylitis (AS), in comparison with a healthy cohort and rheumatoid arthritis (RA), a rheumatic disease having similar symptoms with early AS. Using CSN-assisted data mining, a palmitic acid pathway was constructed, which may be regulated in AS pathogenesis.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"8 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144701257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Impact of Ambient Gas Property on the Signal of Laser-Induced Breakdown Spectroscopy with Neural Network.","authors":"Yuzhou Song,Zongyu Hou,Chenyu Yan,Weiran Song,Chenwei Zhang,Zhe Wang","doi":"10.1021/acs.analchem.5c02073","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c02073","url":null,"abstract":"Laser-induced breakdown spectroscopy (LIBS) has long been regarded as an ideal analytical technology with the unique capabilities of real-time and multielement sensing. However, the lack of a clear understanding of the impact of ambient gas properties on the LIBS signal has severely hindered LIBS quantification improvement. We proposed an innovative approach by applying neural networks to discover the dependence of the LIBS signal on the ambient gas properties supported with a series of purposely designed experiments. For the first time, the full picture of the dependence of the LIBS signal on the main gas properties was clearly discovered, and the impact mechanism was further clarified. It is not only the first time that AI was used for complicated physical dependence rather than quantification in LIBS and the spectroscopic field but also established a new paradigm for the application of AI in complicated physical dependence by constructing comprehensive data points that are virtually impossible to attain through traditional experimental methods.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"55 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144701258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Single-Molecule Fluorescence Lifetime Probe for Apoptosis Diagnosis Through Mitochondrial SO2 and DNA Co-Detection.","authors":"Hong Wang,Tong Zhu,Wanying Li,Zhangjun Hu,Xin Yang,Qiong Zhang,Peng Huang,Jiwen Hu,Zhihui Feng","doi":"10.1021/acs.analchem.5c02360","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c02360","url":null,"abstract":"As a key signaling molecule, sulfur dioxide (SO2) plays a crucial role in maintaining physiological homeostasis. In this work, we designed a mitochondria-targeted fluorescent probe (MIC) for independently detect SO2 (short-wavelength) and DNA (long-wavelength) in real time. It is worth noting that our research reveals a dual role of that SO2 in regulating oxidative stress. At appropriate levels, it acts as a modulator, whereas excessive amounts induce apoptosis by reducing mitochondrial membrane potential, upregulating p53/Bax, downregulating Bcl-2, and activating the caspase-3 cascade. The apoptotic process induces mitochondrial membrane permeability changes, triggering the release and nuclear translocation of the probe MIC, which subsequently emits red fluorescence. Using time-resolved fluorescence imaging technology, we further observed a significant increase in the average fluorescence lifetime of the DNA channel. This finding demonstrates that the MIC probe not only enables dual-channel monitoring of mitochondrial SO2 and nuclear DNA but also precisely evaluates the apoptotic process through changes in fluorescence lifetime. This innovative tool provides an important means for in-depth study of the SO2-mediated apoptotic mechanism and opens up new strategies for the diagnosis of mitochondrial-related diseases.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"24 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144701259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Simultaneous Production Strategies for Adenoviral Vector-Based SARS-CoV-2 Multivalent Vaccines via an Expanded Allele-specific Competitive Blocker PCR Quantification Method.","authors":"Soojeong Chang,Hyemin Park,Jieun Shin,Seowoo Park,Bongju Park,Chang-Yuil Kang","doi":"10.1021/acs.analchem.5c01281","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01281","url":null,"abstract":"As severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to evolve, developing multivalent vaccines becomes crucial to ensure broader immunogenicity against the emerging variants. Traditional multivalent vaccines are produced by combining separately formulated monovalent vaccines in equal proportions, which necessitates precisely quantifying each component. However, the minimal differences in SARS-CoV-2 spike protein sequences among variants pose challenges for immunological quantification methods. In this study, we established an allele-specific competitive blocker polymerase chain reaction (ACB-PCR)-based quantification method to accurately determine the composition of a multivalent adenoviral vector-based SARS-CoV-2 vaccine. By designing primers tailored to each variant and integrating a blocker for target-specific amplification, we achieved high accuracy in distinguishing closely related spike protein variants. Additionally, we explored coinfection and cotransfection strategies as alternative approaches for the simultaneous production of multivalent vaccines. Our results indicated that both these methods maintained antigen composition within a percentage error of approximately 10%, thereby supporting their feasibility for large-scale vaccine manufacturing. This study provides a robust molecular quantification tool for multivalent vaccine analysis and highlights efficient coproduction strategies that could enhance vaccine manufacturing scalability and responsiveness to emerging SARS-CoV-2 variants.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"115 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144701254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indirect Zero-Field Nuclear Magnetic Resonance Spectroscopy.","authors":"Kai Buckenmaier,Richard Neumann,Friedemann Bullinger,Nicolas Kempf,Pavel Povolni,Jörn Engelmann,Judith Samlow,Jan-Bernd Hövener,Klaus Scheffler,Adam Ortmeier,Markus Plaumann,Rainer Körber,Thomas Theis,Andrey N Pravdivtsev","doi":"10.1021/acs.analchem.5c00874","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00874","url":null,"abstract":"This study develops the two-field correlation spectroscopy (COSY) in zero to ultralow field (ZULF) liquid state nuclear magnetic resonance (NMR). We demonstrated the successful integration of signal amplification by reversible exchange (SABRE) hyperpolarization with two-dimensional (2D) NMR spectroscopy, enabling the detection of ZULF COSY spectra with increased sensitivity. Field cycling allowed the acquisition of two-field COSY spectra at varying magnetic field strengths, including zero-field conditions. This enabled insight into both J-coupling and Zeeman-dominated regimes, benefiting from ultralow field observation sensitivity and mitigating the low-frequency noise by conducting readout at higher fields (>5 μT). Our study explores the effects of polarization transfer, apodization techniques, and the potential for further application of ZULF NMR in chemical analysis exemplified for three X-nuclei and three corresponding molecules: [1-13C]pyruvate, [15N]acetonitrile, and [3-19F]pyridine. These findings pave the way for more sensitive and cost-effective NMR spectroscopy in low-field regimes.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"47 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144701256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chiral Carbon Dots as Optical Probes: Selective Detection of Acetylcholinesterase via Enhanced Photoluminescence.","authors":"Jingtao Huang,Ying Ying Nie,Yu Meng Yang,Xin Ying Long,Yongpeng He,Lei Zhan,Cheng Zhi Huang","doi":"10.1021/acs.analchem.5c00073","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00073","url":null,"abstract":"Acetylcholinesterase (AChE), one of nature's most efficient enzymes, plays a crucial role in neural signal transduction and the restoration of tissue homeostasis with implications in the development of neurodegenerative diseases. Conventional methods for detecting AChE mainly rely on its catalytic activity. Herein, we present an approach for AChE detection based on its chiral binding with photoluminescent carbon dots (CDs) with rich chiral cysteine residues (L/D-CDs), synthesized via a general hydrothermal route. Interestingly, L-CDs, despite being synthesized under the same conditions as D-CDs, exhibited a stronger specific binding affinity for AChE than D-CDs through electrostatic interactions, attributed to the amphiphilic nature of AChE, leading to enhanced photoluminescence near the isoelectric point of AChE at 6.50. The enhanced photoluminescence intensity of L-CDs showed a linear correlation with AChE concentrations over the range of 200-4500 mU/mL with a detection limit of 20 mU/mL. Meanwhile, by leveraging the selective recognition of AChE by L-CDs, we applied the probe to distinguish between liver cancer tissues and adjacent nontumor tissues. The results demonstrated that, due to the reduced AChE content in liver cancer tissues, the fluorescence intensity was significantly lower than that in adjacent nontumor tissues. Our findings highlight the potential of CDs rich in chiral residuals as optical probes for biosensing and imaging applications based on biomolecular recognition.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"19 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144701196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Visible Light-Driven Direct Colorimetric Detection of Nitrite with 3,3',5,5'-Tetramethylbenzidine.","authors":"Xianming Li,Ling Li,Heye Lv,Xiaoming Jiang,Peng Wu","doi":"10.1021/acs.analchem.5c02603","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c02603","url":null,"abstract":"Due to the unique features of 3,3',5,5'-tetramethylbenzidine (TMB) in analytical chemistry, a series of TMB-based colorimetric nitrite assays (particularly nanozyme-involved) were developed to supplement the classical Griess assay. However, the exact reaction mechanisms and the final product between TMB and NO2- are controversial. Herein, we found that the widely adopted mechanism, namely the reaction between NO2- and the one-electron oxidation product of TMB (TMB+•), may be not valid. Alternatively, we found the reaction between NO2- and TMB primarily proceeded via diazotization (confirmed with ESI-MS) and yielded yellow diazo-TMB (not TMB2+, the two-electron oxidation product of TMB). In the presence of an easily ignored condition of visible light, diazo-TMB was promoted to its excited state, followed by two photoreactions to yield blue TMB+•, namely photoreduction ([diazo-TMB]* + TMB → TMB+•) and photosensitized oxidation ([diazo-TMB]* + O2 → 1O2, 1O2 + TMB → TMB+•). To facilitate the colorimetric process, a blue LED with a lighting wavelength matching with the maximum absorption of diazo-TMB, was employed to accelerate the generation of TMB+•. The light-driven colorimetric nitrite assay offered a limit of detection (LOD) of 0.1 μM, which was further explored to nitrite detection in urine samples and diagnosis of urinary tract infections caused by Escherichia coli.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"710 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144701197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On-Demand Injection of Microfluidic Droplets Based on Three-Dimensional Visual Feedback Control for High Volume Consistency and Precise Additive Concentration.","authors":"Xiudong Duan,Yingdong Luo,Yuanyuan Huang,Desheng Li,Yuzhe Liu,Hongqiyuan Wang,Chaolong Song","doi":"10.1021/acs.analchem.5c01502","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01502","url":null,"abstract":"Precise and quantitative fluid addition is essential for maintaining consistency in volume and concentration across various applications including pharmaceuticals, food production, and biochemical research. Microfluidic droplet technology has emerged as a versatile microreactor for manipulating nanoliter- to picoliter-scale droplets, offering advantages such as reduced reagent consumption, faster reactions, and enhanced sensitivity. Among these, microfluidic droplet injection technology has shown promise for precise reagent addition. However, passive droplet injection methods as well as active injection methods driven by pneumatic, electrical, and acoustic forces often suffer from volume deviation due to external disturbance. Additionally, traditional two-dimensional (2D) measurement methods overlook the droplet's three-dimensional (3D) curvature, leading to insufficient data capture and significant measurement errors. This study introduces a novel on-demand microfluidic droplet injection system integrating quantitative phase imaging (QPI) for 3D visualization serving as feedback with a dual-pressure-pulse (DPP) method for fluid actuation. Comparative experiments confirmed that our approach significantly improves injection precision, achieving a coefficient of variation (CV) of 7.03%, which represents a 4.5-fold improvement over passive methods. Dynamic response experiments further verified the system's capability to adapt to target volume changes rapidly, maintaining deviations below 2% across varying conditions. As a proof of concept, the system effectively compensates for initial volume fluctuations, ensuring consistent final droplet volumes and enabling controlled isoconcentration of selenium-containing droplets, with deviations of 1.17% and 2.5%, respectively. These findings showcase the system's potential for applications requiring stringent control of volume and concentration, such as single-cell analysis, enzyme kinetics, drug delivery, and food production.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"31 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144701042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}