Analytical Chemistry最新文献

{"title":"A Rapid Testing Kit for 6PPD Screening in Rubber Products","authors":"Zhuoyuan Li, Linna Xie, Pranav Nair, Kirsten Yeung, Oliver Meek, Juan Tao, Kyoshiro Hiki, Chunyan Hao, Hui Peng","doi":"10.1021/acs.analchem.5c00079","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00079","url":null,"abstract":"<i>N</i>-(1,3-dimethylbutyl)-<i>N′</i>-phenyl-<i>p</i>-phenylenediamine (6PPD) has received increasing attention due to its ubiquitous environmental occurrence and extreme aquatic toxicity of its oxidation product 6PPD-quinone. Given 6PPD’s application as an antioxidant in a wide array of rubber products, cost-effective detection of 6PPD is important for product and waste management. We herein developed a rapid testing kit for the detection (&lt;10 min) of 6PPD in rubber products with low costs (&lt;$1/sample), which was inspired by the observation of a red-colored product after 6PPD exposure to oxidants. The selectivity of this reaction was evaluated by 14 structurally diverse phenols and anilines. Interestingly, the protonated <i>N</i>-1,3-dimethyl butyl-<i>N’</i>-phenyl quinone diimine (6QDI+H⁺), rather than its neutral or radical counterparts, appeared to be the red-colored product with λ_max = 490 nm. We further developed a “green” and user-friendly 6PPD rapid testing kit, which was further applied to a wide array of 50 rubber products. 6PPD was detected in 22 out of 50 samples, with a strong agreement (<i>R</i>² = 0.896) observed between the results of the rapid testing kit and LC-MS. In summary, this study introduces a 6PPD rapid testing kit for users without requiring expertise in chemistry.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"76 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Triple-Standard Hypochlorite Quantitative Array Enabled by Precise Stokes Shift Modulation in D-π-A Chemodosimeters","authors":"Mubalake Rehemaitijiang, Gaosheng Li, Rongchao Zhu, Baiyi Zu, Xincun Dou, Zhi Su, Zhenzhen Cai","doi":"10.1021/acs.analchem.5c00821","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00821","url":null,"abstract":"The rational design of the D-π-A chemodosimeter with a significant Stokes shift is of great importance for enhancing the visualization of optical sensing signals. Here, three D-π-A fluorescent chemodosimeters with 2-(3-cyano-4,5,5-trimethylfuran-2(5H)-ylidene) malononitrile (TCF) as the electron-withdrawing group are synthesized by precisely modulating the electron-releasing strength. By decreasing the ability of electron release, the electrophilicity of the recognition site is increased by 1.449 kcal/mol, the Stokes shift of the chemodosimeter is improved to 201 nm, and the sensing mode changes from fluorescence quenching to ratiometric fluorescence and finally to fluorescence on. Furthermore, the three D-π-A fluorescent chemodosimeters display superior sensing performance toward ClO^–, including low limits of detection (LOD, 37.0, 5.1, and 1.0 nM), rapid response (&lt;5 s), and great selectivity in the presence of 16 kinds of interferents. Moreover, the practicality of the chemodosimeters is further validated by a portable triple-standard quantitative array detection platform, which can quantitatively detect ClO^– solutions. The proposed design and modulation strategy for chemodosimeters can provide a new pathway for the sensitive and visualized identification of oxidants and other hazardous chemicals.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"2 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143872939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-Sensitive Biosensor Based on Cell-Derived Nanovesicles for CB1 Receptor-Targeted Drug Development in a Live Cell-Free Platform","authors":"Minwoo Kim, Hyungsup Kim, Solpa Lee, Inje Lim, Eunyoung Kim, Uhtaek Oh, Yongwoo Jang","doi":"10.1021/acs.analchem.4c06959","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c06959","url":null,"abstract":"The endocannabinoid system, particularly the cannabinoid receptor 1 (CB1), is essential for regulating numerous physiological processes, including pain, mood, appetite, and neurodegeneration. Given its crucial role, CB1 has become a target for therapeutic interventions with significant potential for treating various disorders. However, conventional methods such as calcium imaging and patch-clamp can only detect drug concentrations in the nanomolar to micromolar range, highlighting the need to develop more sensitive drug screening methods. To address this issue, we developed an ultrasensitive biosensor based on cell-derived CB1 nanovesicles (CB1-NV) coupled with carbon nanotube (CNT)-printed electrodes. This ultrasensitive sensor can detect cannabinoid compounds at picomolar concentrations by converting receptor-mediated Ca²⁺ influx into measurable electrical signals. The sensor exhibits remarkable sensitivity in terms of detecting trace tetrahydrocannabinol amounts (approximately 0.001%) in hemp seed oil, which conventional methods fail to detect. Compared with conventional methods, the developed biosensor exhibited a 1000-fold improvement in sensitivity, offering a promising tool for high-throughput drug screening and therapeutic research. Additionally, the CB1-NV sensor utilizes cell-free vesicles to preserve the cellular environment. However, because live cells were not involved, there was no requirement to maintain cell viability during the measurement process.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"7 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fast NMR-Based Assessment of Cancer-Associated Protein Glycosylations from Serum Samples","authors":"Lorena Rudolph, Renia Krellmann, Darko Castven, Lina Jegodzinski, Helena Deriš, Jerko Štambuk, Jarne Mölbitz, Luna Dechent, Kai Sperling, Melissa Lind loge, Nele Friedrich, Franziska Schmelter, Bandik Föh, Irena Trbojević-Akmačić, Christian Sina, Matthias Nauck, Astrid Petersmann, Jens U. Marquardt, Ulrich L. Günther, Alvaro Mallagaray","doi":"10.1021/acs.analchem.5c00285","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00285","url":null,"abstract":"Nuclear magnetic resonance (NMR) spectra of blood serum and plasma show signals arising from metabolites, lipoproteins, and <i>N</i>-acetyl methyl groups of <i>N</i>-glycans covalently linked to acute-phase proteins. These glycan signals often called glycoprotein A (GlycA) and glycoprotein B (GlycB) arise from <i>N</i>-acetyl methyl groups and have been proposed as biomarkers, initially for cardiovascular diseases, but also for other inflammatory conditions. For the detection of glycan resonances, <i>J</i>-edited, diffusion, and relaxation filtered NMR spectroscopy (JEDI) has been proposed to suppress the lipoprotein signals. JEDI is however limited to measure those acetyl signals, whereas all other glycan resonance cannot be observed. For improved glycoprotein profiling, the signals arising from the pyranose ring protons are essential. Here, we show how selective frequency excitation combined with scalar coupling filtering can be used to dramatically increase the number of <i>N</i>-glycan signals observable in NMR spectra of serum and plasma samples, facilitating glycosylation profiling in less than 30 min. This approach grants selective detection of sialylation, galactosylation, <i>N</i>-acetylglucosaminylation, and fucosylation of dominant <i>N</i>-glycans and, to some extent, <i>N</i>-glycan branching complexity. Notably, sialylated and nonsialylated Lewis^x and Lewis^a antigens can also be observed. Lewis^a antigen is well established as a cancer biomarker, known as CA19-9. NMR glycosylation profiles from nine isolated serum glycoproteins show excellent agreement with well-established UHPLC-MS analysis. The proposed NMR method facilitates the detection of glycoprotein biomarkers without the need for enzymatic treatment of serum or plasma and provides a robust read-out as exemplified by samples from 33 patients with hepatocellular carcinoma.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"16 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a Self-Assembled DNA Nanofirework for Signal Amplification and Intracellular miRNA Imaging","authors":"Yuyu Tan, Hongye Mao, Jingping Liu, Hui Chen, Jian Yang, Jiaoli Wang, Jin Huang","doi":"10.1021/acs.analchem.5c01369","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01369","url":null,"abstract":"Nonenzymatic DNA catalytic amplification strategies have greatly improved the detection of biomolecules. However, the membrane barrier and the complex intracellular environment remain the two main challenges for efficient intracellular RNA imaging. Herein, we designed a self-assembled DNA nanofirework for amplified microRNA (miRNA) imaging in living cells. The self-assembled DNA nanofireworks exhibited high sensitivity and specificity for miRNA detection, achieving excellent internalization efficiency through endocytosis, while demonstrating enhanced biostability and biocompatibility. These properties enable powerful signal-amplified miRNA imaging in live cells. Under optimized conditions, this nanoprobe achieves a linear detection range of 0.5–8.0 nM for miRNA-21 with a detection limit of 89.3 pM. This design represents an optimal integration of DNA nanotechnology with nucleic acid amplification for intracellular biomolecule analysis, providing valuable insights for understanding the biological functions of important biomolecules in disease pathogenesis and potential therapeutic applications.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"4 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Dual-Capture and Dual-Output 3D DNA Walker System Integrated with Ligases Enables Ultrasensitive Detection of Single-Nucleotide Polymorphisms","authors":"Yunshan Zhang, Qianglong Tan, Fang Yang, Tuo Huang, Siyu Yu, Jing Ye, Jianxian Zeng, Xianzhong Feng, Diming Zhang","doi":"10.1021/acs.analchem.5c00028","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00028","url":null,"abstract":"DNA walkers, as structurally and functionally programmable signal amplification tools, exhibit great potential for application in the field of biosensing. Traditional DNA walkers often rely on enzymes for operation, posing compatibility challenges, while the handful of existing enzyme-free DNA walkers demonstrate limited performance. To address this, we innovatively developed an efficient enzyme-free 3D DNA walker with dual capture and dual output capabilities. Coupled with ligase chain reaction (LCR), this system facilitates highly sensitive and specific detection of single nucleotide polymorphisms (SNPs). Specifically, LCR precisely identifies single-base mutations, effectively transmitting biological information. The 3D DNA walker system is based on entropy-driven circuit cycling reaction technology. In this system, LCR products serve as the driving strands for the DNA walker, independently binding to track strands and walking legs immobilized on gold nanoparticles, forming a unique dual signal capture mechanism. Each track strand carries two signal chains, significantly enhancing signal amplification efficiency. Benefiting from this novel enzyme-free 3D DNA walker strategy, our biosensing system exhibits exceptional sensitivity to mutant targets (MT), detecting MT at concentrations as low as 30.3 aM and distinguishing heterozygous samples with a 0.01% mutation frequency. Furthermore, this system has been successfully applied to genotyping and mutation abundance assessment of genomes from fresh soybean leaves, demonstrating its vast potential for practical applications. In summary, this research pioneers a novel enzyme-free 3D DNA walker with dual capture and dual output capabilities, and develops an ultrasensitive genotyping tool. This provides strong technical support for the advancement of genetic research.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"35 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogen Sulfide-Responsive MRI Probe for Imaging Colon Cancer in Mice","authors":"Yue Sun, Xingyue Fan, Huiyi Liu, Cheng Zhang, Xianzheng Tan, Guosheng Song","doi":"10.1021/acs.analchem.4c07077","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c07077","url":null,"abstract":"Hydrogen sulfide (H₂S), a significant gaseous signaling molecule, is highly expressed in colon cancer. However, realizing highly sensitive and specific imaging of H₂S in deep colon cancer tissues remains an important challenge. In order to overcome this limitation, we have developed a H₂S-responsive magnetic probe (HRMP) with a high sensitivity and specificity. HRMP is synthesized using superparamagnetic iron oxide and Mn-porphyrin, coated with a hydrogen sulfide-responsive polymer. Upon reaction with H₂S, the released nanoparticles aggregate, producing an enhanced transverse relaxivity (<i>r</i>₂) through the dipolar effect. Incorporation of an ortho azide group ensures that HRMP specifically responds to H₂S, reacting swiftly within 2 h to induce a change in <i>T</i>₂ relaxation time. Additionally, by precisely tuning the feeding ratio of Mn-porphyrin to iron oxide, HRMP was endowed with high sensitivity, achieving a detection limit as low as 8.7 μM. In studies with HCT116 colon cancer, where H₂S is overexpressed, HRMP generated a distinct negative contrast at the tumor site. HRMP shows potential for in vivo imaging of colon cancer, offering promise for the early diagnosis of tumors.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"7 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143872936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating the Preparation of a Tissue Section on Adhesive Tape with an Adsorption Platform Device for Simplified Ambient Mass Spectrometry Imaging Analysis","authors":"Yingfeng Xue, Jinyi Li, Zhehui Zhao, Jiaheng Li, Xin Li, Ruiping Zhang, Ling Ren, Lulu Wang, Wenxuan Zhang, Zhigang Luo, Zeper Abliz","doi":"10.1021/acs.analchem.5c01648","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01648","url":null,"abstract":"As a visualization technology for the in situ characterization of surface material molecules, mass spectrometry imaging (MSI) analysis is being increasingly used in various fields, especially in the biomedical field. However, the preparation of biological tissue section samples for MSI analysis remains time-consuming and labor-intensive, and sample loss or damage occurs frequently. The inability to stably and consecutively obtain suitable section samples and perform concise and efficient imaging analysis limits the analysis throughput. Herein, a preparation method is proposed. It enables consecutive sectioning, batch preservation, and dry processing through the use of ordinary adhesive tape, enhancing the adhesion between section and tape and rapid freeze-drying. Furthermore, based on the air flow assisted desorption electrospray ionization (AFADESI) MSI system, a vacuum adsorption platform is introduced, which simplifies the process of MSI analysis. Moreover, compared with general tape-based MSI methods, the signal intensity of 73%–85% of the annotated ions is improved for positive ion mode. The signal-to-noise (<i>S</i>/<i>N</i>) ratios of characteristic ions in the corresponding regions in the images of the tissue section samples increase by an average of more than two times, and a clearer organ outline can be seen in the images. By integrating the sample preparation method with the adsorption platform, high-throughput imaging of serial whole-body or scattered organ tissue sections can be conducted more easily and concise and efficient MSI analysis can be performed, which will provide a new strategy to meet rapidly growing MSI research demands.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"74 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143872938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling Antibacterial Mechanisms of Surfactants against Staphylococcus aureus via Single-Cell Raman Spectroscopy","authors":"Xiaoshan Zheng, Rui Li, Ting Wang, Xunrong Li, Xiao Han, Yajie Dai, Jiquan Liu, Jian Xu","doi":"10.1021/acs.analchem.4c06380","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c06380","url":null,"abstract":"Antibacterial agents, particularly surfactants, play crucial roles in the personal and home care industries. However, current methods for assessing their efficacy and mechanism are commonly time-consuming and expensive. Here, we established a ramanome-based approach to investigate the antibacterial mechanisms of cationic and anionic surfactants with varying alkyl chain lengths against <i>Staphylococcus aureus</i> as a model. Our findings further elucidate the synergy between anionic surfactants and acidic pH. Cell membrane integrity was disrupted by all of the surfactants, as revealed by the decrease in Raman bands assigned to major cellular components (nucleic acids, proteins, and cytochrome), leading to the leakage of cellular components. Moreover, the composition of the cell membrane was altered due to insertion of cationic surfactant, evidenced by the emergence of surfactant-characteristic bands in the spectrum of <i>S. aureus</i>; yet this was observed only under acidic conditions for anionic surfactants. Remarkably, changes in Raman bands of staphyloxanthin and S═O which are biomarkers of cellular oxidative states revealed that acidic conditions accelerated cell death induced by the anionic surfactant. These findings illustrate distinct mechanisms of action for cationic and anionic surfactants and suggest that ramanomics offers a rapid, low-cost, comprehensive, and mechanism-revealing approach for the assessment and screening of surfactants.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"14 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143872935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Integrated Nanosensor/Smartphone Platform for Point-of-Care Biomonitoring of Human Exposure to Pesticides","authors":"Hussian Maanaki, Letice Bussiere, Aleksandr Smirnov, Xiuxia Du, Yu Sun, Thomas A. Arcury, Phillip Summers, Landon Butler, Carey Pope, Anna Jensen, Gregory D. Kearney, Joshua T. Butcher, Jun Wang","doi":"10.1021/acs.analchem.4c06421","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c06421","url":null,"abstract":"Organophosphorus (OP) compounds are neurotoxins that are among the most widely used pesticides in agriculture in the United States. In this application, a new integrated point-of-care smartphone/resistive nanosensor device is developed for onsite rapid and sensitive detection of exposure to OP pesticides from a drop of finger-stick blood among a sample of farmworkers. The nanosensor leverages the transport properties of a multiwalled carbon nanotube/polyaniline nanofiber (MWCNT/PAnNF) nanocomposite film on a gold interdigitated electrode and acetylcholinesterase/butyrylcholinesterase (AChE/BChE) hydrolysis of their respective substrates generating protons doping PAnNFs, thereby increasing the conductance of the film. As such, a conductance change can be used to quantify cholinesterase activity, enabling assessment of acute/chronic OP poisoning. Additionally, a mobile app was developed for the nanosensor to process, display, track, and share results. Under optimal conditions, the nanosensor demonstrated exceptional sensitivity with the detection limits of 0.11 U/mL for AChE and 0.093 U/mL for BChE, physiologically relevant dynamic ranges of 2.0–18.0 U/mL for AChE and 0.5–5.0 U/mL for BChE in whole blood, and high reproducibility with the relative standard variation of &lt;4%. The nanosensor was further validated with widely used radiometric and Ellman’s methods, utilizing both <i>in vitro</i> pesticide-spiked blood samples and blood samples from 22 farmworkers. The results between this nanosensor and those two methods demonstrated a strong agreement. This platform provides a new avenue for the simple, rapid, and sensitive biomonitoring of OP pesticide exposure.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"9 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}