Analytical Chemistry最新文献

{"title":"Enhanced Intracellular Delivery via Stainless Steel Filters and Viscoelastic Fluids: A High-Efficiency Alternative to Conventional Transfection","authors":"Qiang Zhao, Partha Pratim Sarkar, Ye Ai","doi":"10.1021/acs.analchem.5c02251","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c02251","url":null,"abstract":"Advancements in intracellular delivery have garnered significant attention due to their potential applications in the biological and medical fields. Traditional methods, including virus infection, electroporation, and lipofection, have limitations such as toxicity, high costs, and low delivery efficiency. This study presents a novel intracellular delivery method utilizing a stainless-steel filter combined with poly(ethylene oxide) (PEO) solution to transport biomolecules into cells. Optimal parameters were identified through systematic adjustments using fluorescein isothiocyanate (FITC)-dextran. The performance was further evaluated by mRNA encoding enhanced green fluorescent protein (eGFP), demonstrating a remarkable delivery efficiency of 94.7%, a cell viability rate of 94.3%, and a throughput of 1 × 10⁷ cells/min. This new approach not only provides a cost-effective and user-friendly alternative but also enhances the potential for high-throughput applications in biological research and therapeutic interventions. The findings suggest that the integration of stainless-steel filters and viscoelastic fluids enhances intracellular delivery capabilities, positioning this method as a promising tool for advancing cellular engineering technologies.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"28 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144189005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiplexed lncRNA Analysis in Breast Cancer via Cascade Amplification Reaction through Fluorescent and Electrochemical Dual-Signal Output Modalities","authors":"Liming Wang, Li Zhao, Shiyu He, Huijun Qiao, Xinyi Zhang, Xinyan Li, Mengzhe Guo, Fenglei Gao, Shibao Li, Shuguang Ge, Yanyan Yu","doi":"10.1021/acs.analchem.5c01156","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01156","url":null,"abstract":"Long noncoding RNAs (lncRNAs) have increasingly come to the forefront as promising blood biomarkers in the realm of cancer diagnosis and treatment, sparking intense interest in early cancer detection. Nevertheless, considering that cancer is a complex, multistage disease, the detection of a single lncRNA alone is insufficient to precisely mirror the progression of the disease. In the present study, we devised a sophisticated dual-mode sensing strategy that ingeniously combined fluorescent and electrochemical modalities through a cascade amplification reaction, which allowed for the concurrent identification of lncRNA MALAT1 and HOTAIR. In the presence of MALAT1 and HOTAIR, the corresponding DNAzyme activities were activated. Under the impetus of Mg²⁺, the DNAzymes cleaved specific sites on their substrates, thereby generating a copious amount of trigger sequences (T). Subsequently, these trigger sequences were isolated via magnetic separation and then participated in downstream toehold-mediated strand displacement (TMSD) reactions. With the assistance of fuel strands, the cyclic reactions gave rise to substantial fluorescent signals from labeled FAM and Cy3, as well as electrochemical responses from labeled methylene blue (MB) and ferrocene (Fc), thus facilitating the fluorescent/electrochemical dual-mode sensing. The limits of detection as low as 6.3 fM for MALAT1 and 15.2 fM for HOTAIR have been achieved. Significantly, as a proof of concept and preliminary feasibility exploration, this method has been applied to quantitatively assess the levels of MALAT1 and HOTAIR in multiple cancer cells and 13 whole blood samples from breast cancer patients, which demonstrated high levels of consistency with those obtained by real-time quantitative polymerase chain reaction (qRT-PCR) test kits.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"58 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144189007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intramolecular Electrochemiluminescence Resonant Energy Transfer Biosensor Utilizing Ir-Grafted 2D Hf-MOL and Circular DNA Walker for Ultrasensitive Detection of microRNA-21 in Non-Small-Cell Lung Cancer Diagnostics","authors":"Binnan Shi, Luyang Lv, Dehao Jia, Zhuangzhuang Ru, Shuyuan Liu, Yu Du, Jingshuai Li, Qin Wei","doi":"10.1021/acs.analchem.5c02322","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c02322","url":null,"abstract":"The rapid advancement in non-small-cell lung cancer (NSCLC) diagnostics demands ultrasensitive biosensors for detecting microRNA-21 (miRNA-21), a pivotal prognostic biomarker. An innovative electrochemiluminescence (ECL) biosensor was developed that leverages ECL resonance energy transfer (ECL-RET) as the primary amplification mechanism for ultrasensitive detection. The biosensor integrated a two-dimensional hafnium-based metal–organic layer (<b>Hf-MOL</b>) functionalized with iridium complexes (<b>Ir-Hf-MOL</b>), which serves as an efficient ECL emitter. Enhanced sensitivity is achieved through precise spectral overlap and Förster radius optimization, enabling stable energy transfer from <b>Hf-MOL</b> to Ir-COOH. The structural rigidity of <b>Ir-Hf-MOL</b> further contributed to suppressing nonradiative decay through restricted molecular motion. Charge transfer feasibility is validated via density functional theory (DFT), while a circular DNA walker-mediated amplification strategy is incorporated to augment specificity and signal amplification. The optimized system achieves an exceptional linear range from 1 aM to 1 nM, with a low detection limit of 0.76 aM. Rigorous evaluations of selectivity, stability, and recovery rates (99.4–103.2%) in human serum and Bland–Altman plots confirm clinical applicability. This work establishes a transformative biosensing platform for miRNA-21 detection, emphasizing ECL-RET-driven innovation, and advances diagnostic strategies for NSCLC.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"58 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144184010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tri-Recognition-Mediated Proximity Ligation for Quantitative Analysis of Exosomal Protein-Specific Sialylation and Application on a Microfluidic Platform","authors":"Hongfeng Lu, Zhen Gu, Yilin Zou, Hua Wang, Siyu Lu, Huifeng Wang, Bang-Ce Ye, Huiying Xu","doi":"10.1021/acs.analchem.5c01817","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01817","url":null,"abstract":"Overexpression of sialylated glycoprotein is a stage-specific process and is regarded as a common manifestation of tumor progression. Accurate quantification of protein-specific sialylation on biological membranes contributes to a thorough comprehension of cellular signal transduction as well as the search for sialylated glycan-related biomarkers. Herein, we propose triple recognition-mediated proximity ligation coupled with rolling-circle amplification to examine protein-specific sialylation on living cell membranes and their derived exosomes. Multiple recognitions in spatial proximity provide three key advantages: (1) significantly improved identification precision, (2) flexible and scalable target options, and (3) minimized off-target effects. Using this approach, we successfully visualize sialylation-dependent interactions between exosomes and cells. By converting certain recognition sites and combining duplex calculations, we establish a quantitation method of exosomal protein-specific sialylation capping ratio, which could act as a useful noninvasive indicator in a customizable 3D-printed microfluidic chip (ExoTRAP) for exosome-based cancer discrimination. This platform enables multiplexed profiling of protein-specific sialylation with high sensitivity (e.g., the LOD of sialylated MUC1-positive MCF-7 exosomes is 2.81 × 10⁶ particles/mL), thus providing new insights into the role of sialylated glycoproteins in exosome functions, as well as a promising strategy for clinical diagnosis.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"33 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144189011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aminopeptidase N-Triggered Electron Rearrangement of Fluorescein for Covalent Fluorescent Labeling and Image-Guided Orthotopic Bladder Cancer Resection","authors":"Bing Wang, Chaoxiang He, Yueqing Li, Yihan Jiang, Xiongjie Li, Zonglong Li, Changzhen Peng, Haidong Li, Xiaojun Peng, Xiaoqun Zheng, Feng Xu","doi":"10.1021/acs.analchem.5c02393","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c02393","url":null,"abstract":"Bladder cancer is the most common type of malignant tumor in the urinary system, which occurs in the bladder mucosa. Due to the lack of precise diagnostic tools, bladder cancer has a high recurrence rate. Fluorescence cystoscopy is recognized as a promising tool for improving the detection rate of bladder cancer, but its clinical application is hindered due to the scarcity of fluorescent agents. To address this issue, an aminopeptidase N (APN)-activated fluorescent agent with specific recognition and labeling abilities for bladder cancer has been developed. The fluorescent agent, namely, Flu-FAPN, exhibited high sensitivity and specificity toward APN under physiological conditions. The fluorescent intensity increased more than 37-fold after reacting with APN, and the detection limit is 0.10 ng/mL for APN. In addition, the hydrolysis of alanine catalyzed by APN could initiate the intramolecular electron rearrangement process, which subsequently contributed to the formation of protein-fluorescein adduct and thereby achieved covalent labeling of bladder cancer cells. Cell experiments conducted in cancer and normal cells demonstrate that Flu-FAPN has low cytotoxicity and high specificity for bladder cancer cells with an obvious fluorescence signal, which could be retained over 80% even in fixed cells. Finally, Flu-FAPN was successfully applied for the fluorescence-image-guided resection of tumor tissues in mice with orthotopic bladder cancer. We hope this covalent labeling-based agent can provide a promising tool for surgery navigation and accelerate the clinical application of fluorescence cystoscopy, thereby improving the cure rate of bladder cancer.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"11 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144184013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Cell Membrane-Anchored Aptamer Sensor for IL-2 Detection In Situ","authors":"Li-Ting Yang, Hang Zhou, Shun Li, Xiu-Song Huang, Tuersunayi Abudureheman, Hua Zhu, Jian-Min Zhu, Jing Chen, Cai-Wen Duan, Kai-Ming Chen","doi":"10.1021/acs.analchem.5c01030","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01030","url":null,"abstract":"Interleukin-2 (IL-2) is a pivotal cytokine that plays a crucial role in the activation, proliferation, and functional regulation of multiple immune cells. High-dose IL-2 has been approved for antitumor therapy but may cause toxicity. IL-2 levels within the body are also associated with various pathological states, making it a potential diagnostic and prognostic biomarker. Consequently, the development of efficient tools for the rapid detection and real-time quantification of IL-2 is of great significance. In this study, we identified two human IL-2-binding aptamers, Apt24 and Apt35, through the systematic evolution of ligands by exponential enrichment (SELEX). On this basis, we constructed two aptamer beacons to detect IL-2 proteins in solution. Further, we successfully developed a membrane-anchored aptamer sensor, Chol-Apt35, to realize in situ detection of IL-2 secretion in living cells. The sensor exhibits good membrane-insertion ability and performs comparably with fluorescent antibodies. In conclusion, this study provides simple yet effective tools for IL-2 detection, which may facilitate dose optimization in immunotherapy and assessment of immunological status.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"11 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144184015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toward “Receive-Only” Nuclear Magnetic Resonance Complementary Metal-Oxide-Semiconductor Microcoil Arrays for High-Throughput Analysis of Environmental Samples","authors":"Daniel H. Lysak, Carl A. Michal, Kathryn Marable, Marco Grisi, Reza Farsi, Peter M. Costa, Jacob Pellizzari, Vincent Moxley-Paquette, Katelyn Downey, Kiera Ronda, Owen Vander Meulen, William W. Wolff, Katrina Steiner, Flavio V. C. Kock, Tiago B. Moraes, Luiz A. Colnago, Felix Schuderer, Tobias Wirth, Myrna Simpson, Jens Anders, Andre J. Simpson","doi":"10.1021/acs.analchem.5c00955","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00955","url":null,"abstract":"<i>Daphnia magna</i> neonates and eggs are critical life stages that show different susceptibilities to toxins and stressors compared to adults. Nuclear magnetic resonance (NMR) spectroscopy has unique potential to uncover the underlying biochemical causes, but such very small, mass-limited samples are challenging to study. While the enhanced mass sensitivity of microcoils leads to markedly improved NMR analysis, experiments are often still long, leading to low throughput. Microcoil arrays can improve throughput by allowing concurrent analysis of multiple samples, but practical and economical challenges, including expensive receivers and lack of physical space, are prohibitive. Here, approaches to overcome these challenges were examined. First, “receive-only” planar complementary metal-oxide-semiconductor (CMOS) microcoils were explored wherein the microcoil was used for detection, but an external volume coil was used for excitation. This resulted in considerably improved nutation and provided a 70% boost to the signal-to-noise ratio (SNR) compared to excitation on the planar CMOS coil. Steady-state free precession experiments further improved the SNR by a factor of at least 2.5, reducing acquisition time and improving throughput. To complete a three-coil “receive-only” array, an inexpensive ($315) software-defined radio board was adapted to act as an NMR receiver with similar performance to commercial receivers. The three-coil array was used to study three <i>D. magna</i> eggs concurrently, potentially opening the door for high-throughput analysis and toxicological studies, where the control and exposed cohorts can be studied concurrently, reducing day-to-day variability. Overall, microcoil arrays show considerable promise for the study of <i>D. magna</i> eggs and other mass-limited samples.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"28 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144184078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the Amplification-Free Quantitative Detection of Viral RNA in Nasopharyngeal Swab Samples Using a Compact Electrochemical Rapid Test Device","authors":"Manuel Gutiérrez-Capitán, Eva Balada, Anna Aviñó, Lluïsa Vilaplana, Roger Galve, Alícia Lacoma, Antonio Baldi, Antonio Alcamí, Véronique Noé, Carlos J. Ciudad, Ramón Eritja, María-Pilar Marco, César Fernández-Sánchez","doi":"10.1021/acs.analchem.5c01605","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01605","url":null,"abstract":"Providing viral load numbers of infection events aids in the identification of disease severity and in the effective overall patient management. Gold-standard polymerase chain reaction (PCR) techniques make this possible but cannot be applied at the point of need and in low-resource settings. Here, we report on the development of a compact analytical platform that can detect a conserved sequence of the RNA of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) in 40 min in nasopharyngeal swab samples without the need for any previous purification or gene amplification steps. It combines electrochemical and paper fluidic approaches together with a sandwich hybridization assay performed on magnetic nanoparticles (MNPs) modified with a tailor-designed capture DNA hairpin. The device proves to quantitatively detect viral RNA in a retrospective study carried out with nasopharyngeal swab samples. A sensitivity of 100% and a specificity of 93% were estimated by the receiver operating characteristic (ROC) analysis. However, although molar concentration values of the target RNA sequence are provided, these estimates do not fully correlate with the viral load numbers estimated by RT-qPCR over the whole Ct sample range. Empirical studies have been carried out that have provided clear insights into this hurdle and simple solutions to overcome it, without depriving the device of the features required for potential use in a point-of-care (PoC) environment.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"71 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144177198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fusion of Molecular and Mechanical Phenotypes Enables High-Purity and Low-Loss Reacquisition of Viable CTCs for Transcriptome Analysis","authors":"Haibin Si, Qing Xu, Yan Sun, Dexin Du, Yiguo Wang, Simin Li, Lu Li, Bo Tang","doi":"10.1021/acs.analchem.5c01806","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01806","url":null,"abstract":"The high-purity and low-loss reacquisition of viable circulating tumor cells (CTCs) is crucial for enabling downstream omics analysis of CTCs and currently represents key challenges limiting their application in clinical diagnosis and pathological research. Given the limitations of traditional methods that rely solely on a molecular or mechanical phenotype for CTCs acquisition, this study introduces an innovative approach that fuses the inherent molecular and mechanical phenotypes of CTCs into a new mechanical phenotype, thereby achieving high-purity preconcentration and low-loss reacquisition of CTCs. Specifically, CTCs in blood are immunomodified using calcium carbonate microspheres (CCMSs) conjugated with antibodies, transforming the molecular phenotype (membrane protein expression) into an additional mechanical phenotype (increased size and reduced deformability). This transformation enhances the mechanical phenotype distinctions between CTCs and white blood cells, enabling high-purity preconcentration of CTCs on a single-cell trapping array chip. Since CCMSs can be reversibly eliminated under weak acid, captured CTCs can be nondestructively reacquired with 93.10% in microliter-scale solution, allowing for subsequent omics analysis. In a breast cancer mouse model, the counts and transcriptome analysis of CTCs provide valuable insights into assessing tumor occurrence and progression.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"43 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144177199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual Ratiometric Single-Molecule Theranostic Probes for Photothermal Therapy and Real-Time Quantitative Evaluation of Therapeutic Efficacy In Vivo","authors":"Shuping Zhang, Xingyue Liu, Bang-Ping Jiang, Shi-Chen Ji, Hua Chen, Xing-Can Shen","doi":"10.1021/acs.analchem.5c02253","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c02253","url":null,"abstract":"Tracking the quantitative evaluation of therapeutic efficiency in tumors is essential for the precision management of cancer patients. Theranostic probes, which integrate diagnostic molecular imaging and therapeutic capabilities into a single entity, can be used to monitor the treatment process and reflect the therapeutic effect. However, current theranostic probes lack precise quantitative evaluations <i>in vivo</i> due to the use of single-wavelength imaging during tumor therapy. Here we present a portal library of dual ratiometric single-molecule theranostic probes for precise tumor therapy through photothermal therapy (PTT) and quantitative evaluation of tumor cell death <i>in vivo</i> via ratiometric near-infrared fluorescence (NIRF) and ratiometric photoacoustic (PA) imaging. One optimal single-molecule dye is further modified into an activatable probe (AF-1F-NO₂), whose dual ratiometric NIRF/PA signal and photothermal activity are only activated in the presence of a tumor biomarker (nitroreductase). By eliciting a dual ratiometric response (NIRF/PA: NIRF₈₅₀/NIRF₇₅₀; PA₇₇₀/PA₆₇₀) to tumor hypoxia, the new probe acts as a hypoxia-activated PTT theranostic agent, enabling real-time quantitative evaluation <i>in vivo</i> during PTT. Thus, this study not only presents the first dual ratiometric single-molecule theranostic probe for PTT and real-time quantitative evaluation of therapeutic efficacy <i>in vivo</i>, but also opens up a promising paradigm for engineering other single-molecule dual ratiometric theranostic probes in combination with more therapeutic modalities for precision medicine.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"52 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144177200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}