Analytical Chemistry最新文献

Deciphering the Effect of Protein on Different Types of Target Signals in SERS Detection. 在SERS检测中蛋白质对不同类型靶信号的影响。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-06-21 DOI: 10.1021/acs.analchem.5c03127

Shuna Mi,Yuhang Du,Shaofeng Yuan,Hang Yu,Yahui Guo,Yuliang Cheng,Weirong Yao

{"title":"Deciphering the Effect of Protein on Different Types of Target Signals in SERS Detection.","authors":"Shuna Mi,Yuhang Du,Shaofeng Yuan,Hang Yu,Yahui Guo,Yuliang Cheng,Weirong Yao","doi":"10.1021/acs.analchem.5c03127","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c03127","url":null,"abstract":"The matrix effect remains a significant challenge for the practical application of surface-enhanced Raman spectroscopy (SERS), with proteins being one of the most common interfering components. In this study, melamine (strong SERS signals, weak protein binding), rhodamine 6G (R6G; strong SERS signals, strong protein binding), and phenobarbital (weak SERS signals, strong protein binding) were employed as representative target molecules of different types. α-lactalbumin (α-La), β-lactoglobulin (β-Lg), and bovine serum albumin (BSA) were selected as the model proteins. The effects of the protein concentration on SERS performance were systematically evaluated in terms of gold nanoparticle stability and adsorption behavior. At higher protein concentrations, the SERS signals of all three analytes were significantly attenuated with signal suppression intensifying as protein levels increased. This was attributed to the formation of a complete protein corona on the gold nanoparticle surface, which inhibited particle aggregation and reduced the adsorption capacity of analyte. In contrast, at lower protein concentrations, protein-induced aggregation negatively affected the SERS signals of melamine and R6G but enhanced the signal of phenobarbital, which lacks the intrinsic ability to induce gold nanoparticles aggregation. Additionally, the interaction between phenobarbital and proteins facilitated its adsorption onto the SERS substrate, further boosting its weak signal. These findings offer insights into the impact of proteins on SERS signals of different types of targets and provide a new idea for achieving SERS detection of targets such as phenobarbital, which exhibit inherently weak signals due to their inability to induce nanoparticle aggregation on their own, yet possess strong binding affinity with proteins.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"65 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144337454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NIR-II Plastic Particles for Monitoring Intestinal Motility and Microplastic Deposition in Mice NIR-II塑料颗粒监测小鼠肠道运动和微塑料沉积

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-06-21 DOI: 10.1021/acs.analchem.5c01885

Xinxin Tian, Yiqing Yang, Wenjie Gao, Diankai Liu, Xiaohua Li, Huimin Ma, Wen Shi

{"title":"NIR-II Plastic Particles for Monitoring Intestinal Motility and Microplastic Deposition in Mice","authors":"Xinxin Tian, Yiqing Yang, Wenjie Gao, Diankai Liu, Xiaohua Li, Huimin Ma, Wen Shi","doi":"10.1021/acs.analchem.5c01885","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01885","url":null,"abstract":"Plastic products are widely used in daily life, leading to the pervasive presence of microplastics, which have posed great threats to human health. However, the relative lack of methods to observe the movement and distribution of microplastics in living animals has retarded the understanding of their biological effects. Fluorescence imaging in the near-infrared II (NIR-II) window with good tissue penetration has greatly expanded its scope for living animals. In this study, we have developed a NIR-II fluorescence imaging platform using polystyrene particles to dynamically visualize microplastic behavior in the gastrointestinal tract of living mice. After oral administration of the fluorescent particles, NIR-II imaging at a frame rate of 30 fps revealed the intestinal motility with a peristaltic frequency of 0.51 ± 0.02 beats per second and food transit velocity of 1.3 ± 0.2 mm/s in healthy mice, which excreted 99% of the fluorescent particles within 24 h. In contrast, constipated and colitis mice displayed impaired intestinal peristalsis and significantly prolonged intestinal retention of microplastics. Moreover, the method disclosed that long-term feeding of microplastics resulted in persistent retention in the intestines and spleen, as confirmed by hyperspectral imaging. The utility of the proposed NIR-II imaging platform for dynamic tracking in mammals provides critical insight into the roles of microplastics in biological systems.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"608 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144335080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Multifunctional Tetrahedral DNA Nanostructure for the Simultaneous Sensitive Detection and Imaging of miRNA and Zinc Ions in Living Cells 一种多功能四面体DNA纳米结构用于活细胞中miRNA和锌离子的同时灵敏检测和成像

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-06-21 DOI: 10.1021/acs.analchem.5c01674

Qinghong Jiang, Jie Zhou, Yuwei Sha, Mengshi Jiang, Yaqin Chai, Ruo Yuan

{"title":"A Multifunctional Tetrahedral DNA Nanostructure for the Simultaneous Sensitive Detection and Imaging of miRNA and Zinc Ions in Living Cells","authors":"Qinghong Jiang, Jie Zhou, Yuwei Sha, Mengshi Jiang, Yaqin Chai, Ruo Yuan","doi":"10.1021/acs.analchem.5c01674","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01674","url":null,"abstract":"In this study, a multifunctional tetrahedral DNA nanostructure (MTDN) was constructed by assembling different functional modules for the simultaneous sensitive detection and imaging of targets miRNA-21 and Zn2+ in living cells related to liver cancer. Impressively, compared with the traditional detection strategy for miRNA-21 and Zn2+ with low sensitivity, this strategy employs autocatalytic hairpin assembly (ACHA) to rapidly self-assemble into linear tetrahedral DNA nanostructures (LTDNS) by activating the functional module on MTDN to produce two strong fluorescent signals for sensitive detection of miRNA-21 and Zn2+ with detection limits of 0.79 pM and 33.46 nM, respectively, which were much lower than the reported literature. More importantly, LTDNS with a spatial confinement effect and low diffusion coefficient could achieve accurate localization imaging of targets to significantly enhance imaging clarity. The strategy provides an innovative method for simultaneous sensitive detection and accurate imaging of multiple biomarkers and has broad application prospects in early disease diagnosis.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"15 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144335079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Native Top-Down Proteomics of Endogenous Protein Complexes Enabled by Online Two-Dimensional Liquid Chromatography 通过在线二维液相色谱实现内源性蛋白质复合物的自顶向下蛋白质组学

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-06-20 DOI: 10.1021/acs.analchem.5c02341

Matthew S. Fischer, Holden T. Rogers, Emily A. Chapman, Song Jin, Ying Ge

{"title":"Native Top-Down Proteomics of Endogenous Protein Complexes Enabled by Online Two-Dimensional Liquid Chromatography","authors":"Matthew S. Fischer, Holden T. Rogers, Emily A. Chapman, Song Jin, Ying Ge","doi":"10.1021/acs.analchem.5c02341","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c02341","url":null,"abstract":"Protein complexes are essential for virtually all biological processes, yet their structural characterization remains a major challenge due to their heterogeneous, dynamic nature and the complexity of the proteome. Native top-down mass spectrometry (nTDMS) has emerged as a powerful tool for comprehensive structural characterization of purified protein complexes, but its application to endogenous protein complexes in the proteome is challenging and typically requires labor-intensive and time-consuming prefractionation. Here, for the first time, we develop a nondenaturing online two-dimensional liquid chromatography (2D-LC) method for native top-down proteomics (nTDP), enabling high-throughput structural analysis of endogenous protein complexes. The automated, online interfacing of size-exclusion and mixed-bed ion-exchange chromatography achieves high coverage of endogenous protein complexes. We further develop a multistage nTDMS approach that enables comprehensive structural characterization within the chromatographic time scale, capturing intact noncovalent complexes, released subunits/cofactors, and backbone fragments. Our analysis detected 133 native proteoforms and endogenous protein complexes (up to 350 kDa) from human heart tissue in less than 2 h. This work represents a significant technical advancement toward direct, high-throughput structural characterization of endogenous protein complexes from biological mixtures.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"23 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144329125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FRAP4ICBS: FRAP Analysis Method to Evaluate Dominant Effects for Biomolecular Condensate Formation. FRAP4ICBS：评价生物分子凝聚形成优势效应的FRAP分析方法。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-06-20 DOI: 10.1021/acs.analchem.5c01227

Xiaotian Wang,Zhiguang Xiao,Jiahao Niu,Yujie Sun

{"title":"FRAP4ICBS: FRAP Analysis Method to Evaluate Dominant Effects for Biomolecular Condensate Formation.","authors":"Xiaotian Wang,Zhiguang Xiao,Jiahao Niu,Yujie Sun","doi":"10.1021/acs.analchem.5c01227","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01227","url":null,"abstract":"Cells contain a wide variety of membrane-less organelles, whose formation mechanism is believed to be generally based on liquid-liquid phase separation (LLPS). In fact, condensate formation can involve more than one mechanism; interactions with spatially clustered binding sites (ICBS) may contribute alongside LLPS, sometimes dominating and sometimes playing only a partial role. Here, we introduce FRAP4ICBS, a FRAP analysis method based on ICBS mechanism to characterize the dominant formation mechanisms of condensates. FRAP4ICBS can accurately distinguish between the formation mechanisms of condensates as either LLPS- or ICBS-dominant both in vitro and in silico. We also found that condensates that require DNA/RNA involvement are more likely to be based on the ICBS-dominant mechanism. On this basis, we tested the FUS-ERG protein and DNA co-condensates, which was often claimed to be based on LLPS, and demonstrated that it is more likely to be produced by the ICBS-dominant mechanism. In addition, this conclusion is also verified by single particle tracking. Therefore, the FRAP4ICBS can serve as a tool for identifying the potential mechanisms underlying condensate formation, offering support for a deeper understanding of the formation mechanism of condensates.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"44 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144328893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid Screening of Single-Atom Catalyst Synthesis Conditions Using ToF-SIMS and Facet-Dependent Single-Crystal Substrates. 利用ToF-SIMS和面相关单晶衬底快速筛选单原子催化剂合成条件。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-06-20 DOI: 10.1021/acs.analchem.5c00351

Juejing Liu,Yining Wang,Ping Chen,Yadong Zhou,Zheming Wang,Kevin M Rosso,Zihua Zhu,Xin Zhang

{"title":"Rapid Screening of Single-Atom Catalyst Synthesis Conditions Using ToF-SIMS and Facet-Dependent Single-Crystal Substrates.","authors":"Juejing Liu,Yining Wang,Ping Chen,Yadong Zhou,Zheming Wang,Kevin M Rosso,Zihua Zhu,Xin Zhang","doi":"10.1021/acs.analchem.5c00351","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00351","url":null,"abstract":"Single-atom catalysts (SACs) offer superior catalytic performance compared with traditional nanoparticle catalysts but are challenging to develop because of the need for extensive optimization and specialized characterization techniques. This study presents a rapid and versatile method for detecting synthesis conditions and elucidating the deposition mechanisms of SACs on various substrates. By depositing active elements (Au, Cu, Ni, and Rh) on facet-specific single-crystalline substrates (CeO2, TiO2, MgO, and Al2O3) and employing time-of-flight secondary ion mass spectroscopy (ToF-SIMS), we assessed facet-dependent deposition behaviors and identified optimal conditions for solution-based SAC synthesis. ToF-SIMS revealed diverse deposition behaviors depending on the active element, substrate type, and facet, including the formation of single-atom sites, aggregation into clusters, or absence of deposition altogether. These findings, which align with previous reports on specific systems, highlight the technique's ability to rapidly differentiate these outcomes across various materials. Our study demonstrates that ToF-SIMS is a viable tool for the rapid screening of synthesis conditions, contributing to the faster and more efficient development of next-generation single-atom catalysts.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"21 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144328896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CSU-MS2: A Contrastive Learning Framework for Cross-Modal Compound Identification from MS/MS Spectra to Molecular Structures. CSU-MS2：从质谱/质谱到分子结构的跨模态化合物鉴定的对比学习框架。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-06-20 DOI: 10.1021/acs.analchem.5c01594

Ting Xie,Hailiang Zhang,Qiong Yang,Jinyu Sun,Yue Wang,Jia Long,Zhimin Zhang,Hongmei Lu

{"title":"CSU-MS2: A Contrastive Learning Framework for Cross-Modal Compound Identification from MS/MS Spectra to Molecular Structures.","authors":"Ting Xie,Hailiang Zhang,Qiong Yang,Jinyu Sun,Yue Wang,Jia Long,Zhimin Zhang,Hongmei Lu","doi":"10.1021/acs.analchem.5c01594","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01594","url":null,"abstract":"Tandem mass spectrometry (MS/MS) is a cornerstone for compound identification in complex mixtures, but conventional spectral matching approaches face critical limitations due to limited library coverage and matching algorithms. To address this, we propose CSU-MS2 (contrastively spectral-structural Unification framework for MS/MS Spectra and Molecular Structures), a novel framework that bridges MS/MS spectra and molecular structures through cross-modal contrastive learning. CSU-MS2 uniquely integrates an External Space Attention Aggregation (ESA) module to dynamically align spectral and structural features, enabling direct retrieval of molecular candidates from a unified embedding space. The framework is pretrained on large-scale in-silico MS/MS data sets generated by CFM-ID and ICEBERG, followed by fine-tuning on high-quality experimental data. Results show that CSU-MS2 achieves a Recall@1 of 75.45% when matching 1047 spectra against a reference library containing 1,001,047 compounds, significantly surpassing existing methods such as CFM-ID (68.38%), SIRIUS (64.85%), MetFrag (48.59%), and CMSSP (30.47%). Furthermore, rigorous validation on three external data sets spanning human metabolomics (MTBLS265), plant metabolites (PMhub), and the CASMI 2022 challenge demonstrates robust generalizability, with domain-specific retrieval achieving a Recall@10 of 91.67% for blood metabolites. To facilitate compound identification across various domains, we have assembled a Spectrum-searchable Structural Feature Database (SSFDB) from 23 structural databases and deployed an open-source web server supporting customizable cross-modal retrieval. All code, models, and SSFDB are publicly accessible, offering a transformative solution for high-throughput compound identification in metabolomics and beyond.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"22 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144329048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving the Prediction of Antioxidant Activity by Identifying High-Probability Conformations with NMR. 利用核磁共振识别高概率构象改进抗氧化活性预测。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-06-20 DOI: 10.1021/acs.analchem.4c05912

Emily C Heider,Paula Grace Nickles,Dirk Emde,Scott Burt,James K Harper

{"title":"Improving the Prediction of Antioxidant Activity by Identifying High-Probability Conformations with NMR.","authors":"Emily C Heider,Paula Grace Nickles,Dirk Emde,Scott Burt,James K Harper","doi":"10.1021/acs.analchem.4c05912","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05912","url":null,"abstract":"A single-experiment NMR methodology was recently proposed for characterizing chemical structures in solution. This approach employs measured and computed one-bond carbon-carbon J-couplings to establish atomic connectivity, molecular conformation, heteroatom identity, intramolecular hydrogen bonding, and tautomeric form with high confidence. This methodology also identifies mixtures of conformers and assigns relative amounts. The present study applies this approach to establish conformations in the antioxidants trolox and 3-hydroxythiophene-2-carboxylic acid. The ability to accurately assign conformations in antioxidants is a key step needed before computational mechanistic studies can be successfully performed. In the case of antioxidants, this is especially important because at least four mechanisms of activity are feasible, and an incorrect assignment of conformation can alter mechanistic conclusions. Herein, the NMR methodology is shown to clearly select a single most probable conformation for trolox and two highly probable conformations of 3-hydroxythiophene-2-carboxylic acid. The NMR analysis also identified a possible conformational error in prior computational studies involving trolox.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"24 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144328891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Electrochemiluminescence Sensor for Sensitive Detection of Estriol Based on "Three-in-One" Nanocomposite. 基于“三合一”纳米复合材料的新型电化学发光传感器对雌三醇的灵敏检测。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-06-20 DOI: 10.1021/acs.analchem.5c01270

Shuyue Xing,Xuejing Liu,Dawei Fan,Zhongfeng Gao,Hongmin Ma,Huan Wang,Dan Wu,Qin Wei

{"title":"A Novel Electrochemiluminescence Sensor for Sensitive Detection of Estriol Based on \"Three-in-One\" Nanocomposite.","authors":"Shuyue Xing,Xuejing Liu,Dawei Fan,Zhongfeng Gao,Hongmin Ma,Huan Wang,Dan Wu,Qin Wei","doi":"10.1021/acs.analchem.5c01270","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01270","url":null,"abstract":"In this study, chemically polymerized luminol-hydrogen-bonded organic framework (CpL-HOF) is used as the efficient luminophore and POMs@MOFs as the quencher to construct the electrochemiluminescence (ECL) sensor for the ultrasensitive detection of estriol (E3). CpL-HOF is prepared through coordination self-assembly using chemically polymerized luminol (CpL) and three active ligands. The nanoconfinement effect of CpL-HOF exhibits enhanced stability and ECL efficiency compared with the monomer. Mo-based polyoxometalates (Mo POMs) as guests are encapsulated inside a metal-organic framework (Cu-MOF) to synthesize POMs@MOFs nanocomposites with \"three-in-one\" functions. The distinctive host-guest interaction enhances the activity of superoxide dismutase-like (SOD-like) and catalase-like (CAT-like) enzymes, effectively eliminating reactive oxygen species (ROS). Furthermore, POMs@MOFs exhibit electron-rich redox properties, which can further eliminate ROS by valence switching. Therefore, POMs@MOFs can effectively quench the ECL signal of the CpL-HOF and improve the sensitivity of the sensor. Under optimal conditions, the sensor has a wide linear range (100 fg/mL to 200 ng/mL) and a low limit of detection (92.84 fg/mL). The sensor can be applied to the analysis of real samples, with satisfactory results. The ECL mechanism based on the synergistic interaction of the host-guest provides a theoretical foundation for constructing novel ECL sensing platforms.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"5 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144328898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective and Sensitive Dopamine Detection Using the Pictet-Spengler Reaction for Surface-Enhanced Raman Scattering. 利用Pictet-Spengler反应对表面增强拉曼散射进行选择性和敏感的多巴胺检测。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-06-20 DOI: 10.1021/acs.analchem.5c01454

Kunle Li,Xinyu Zheng,Jiancheng Feng,Xing Feng,Yu Zhao

{"title":"Selective and Sensitive Dopamine Detection Using the Pictet-Spengler Reaction for Surface-Enhanced Raman Scattering.","authors":"Kunle Li,Xinyu Zheng,Jiancheng Feng,Xing Feng,Yu Zhao","doi":"10.1021/acs.analchem.5c01454","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01454","url":null,"abstract":"Dopamine (DA) presents significant challenges for direct quantification using surface-enhanced Raman scattering (SERS) due to its relatively small Raman cross section and the complexity of biological fluids. To address these limitations, this study introduces a novel approach combining the Pictet-Spengler (P-S) reaction with SERS for selective and sensitive DA detection. This method involves the preparation of aldehyde-modified layered double hydroxide, which effectively anchors DA molecules via the P-S reaction. This reaction is highly specific to DA, significantly enhancing the detection selectivity by overcoming interference from other molecules. Silver nanoparticles loaded with 4-mercaptophenylboronic acid are used as SERS probes for the indirect quantification of DA. The DA detection method demonstrated stability, achieving a linear response across a concentration range of 10-5-10-10 mol·L-1, with a minimum detection limit of 9.91 × 10-12 mol·L-1 (S/N = 3). The method also exhibited excellent performance in detecting DA in complex environments, such as simulated urine and cerebrospinal fluid samples, highlighting its potential application in diagnosing and monitoring neurological disorders.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"16 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144328892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0