Analytical Chemistry最新文献_第10页

ΔΔG Method for Elucidating Key Control Reactions from Relative Quantification Metabolome Data: Comparative Analysis of Yeast Glycolysis 从相对定量代谢组数据中阐明关键控制反应的 ΔΔG 方法：酵母糖酵解的比较分析

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-03-22 DOI: 10.1021/acs.analchem.4c04480

Fumio Matsuda, Ayumu Kamiyama, Kazuki Yamasaki, Taisuke Seike, Nobuyuki Okahashi

{"title":"ΔΔG Method for Elucidating Key Control Reactions from Relative Quantification Metabolome Data: Comparative Analysis of Yeast Glycolysis","authors":"Fumio Matsuda, Ayumu Kamiyama, Kazuki Yamasaki, Taisuke Seike, Nobuyuki Okahashi","doi":"10.1021/acs.analchem.4c04480","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c04480","url":null,"abstract":"This study introduces the ΔΔG method, a novel approach to analyzing metabolic regulation using relative quantification metabolome data. The method calculates shifts in the Gibbs free energy change (ΔG) in two different metabolic states. Subsequently, key reactions controlling the metabolic flux can be identified by comparing the ΔΔG values to the reaction rates. Two case studies demonstrated the applicability of this method. First, a metabolome data set was obtained from the wild-type and single-gene-deletion mutant strains of Saccharomyces cerevisiae. The ΔΔG values of the glycolytic reactions were calculated between those of the wild-type and each mutant strain. A positive correlation was observed between the ΔΔG values of phosphofructokinase (PFK) and the approximate glycolytic flux levels. These results suggested that PFK regulates glycolytic flux. Moreover, a comparison between S. cerevisiae (Crabtree-positive yeast) and Kluyveromyces marxianus (Crabtree-negative yeast) revealed that S. cerevisiae primarily regulates glycolysis through PFK, whereas K. marxianus employs a more distributed control. The ΔΔG method provides insights into metabolic regulation that are not apparent from metabolite profiles alone and is applicable to various biological systems, particularly for analyzing glycolysis. Furthermore, the simplicity of this method makes it a valuable tool for metabolic engineering and medical research.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"21 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143672627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spectral Analysis for Photon Emission of Rare-Earth Ions in Single Plasmonic Hot Spot

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-03-22 DOI: 10.1021/acs.analchem.4c07105

Xiao-Feng Li, Jingyu Wang, Yu-Ting Wu, Yadong Zhou, Qiang-Qiang Zhu, Peiqing Cai, An Wang, Yan Shi, Le Wang, Shangzhong Jin, Fan-Li Zhang, Jian-Feng Li

{"title":"Spectral Analysis for Photon Emission of Rare-Earth Ions in Single Plasmonic Hot Spot","authors":"Xiao-Feng Li, Jingyu Wang, Yu-Ting Wu, Yadong Zhou, Qiang-Qiang Zhu, Peiqing Cai, An Wang, Yan Shi, Le Wang, Shangzhong Jin, Fan-Li Zhang, Jian-Feng Li","doi":"10.1021/acs.analchem.4c07105","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c07105","url":null,"abstract":"Rare earth ion luminescent materials have attracted extensive attention due to their wide applications in biochemical sensing and bioimaging. However, the low quantum yield and weak luminescence intensity have restricted their further development. Realizing the modulation of rare-earth ions’ emission behavior has become a hot topic in the interdisciplinary fields of materials and chemometrics. Herein, the regulation of the electron decay process and emission of photon signals of rare earth ion (Eu3+) has been achieved in a well-defined plasmonic nanocavity. This nanocavity consists of Ag shell-isolated nanoparticles (SHINs) and an ultraflat Au film, which are separated by a polymer dielectric spacer and CaF2:Eu3+ nanoparticles. Contrary to the intrinsic photoluminescence of CaF2:Eu3+, a factor of 408 increase in the spontaneous emission rate and simultaneously an 800-fold enhancement in the emission intensity have been realized in nanocavities via comprehensive spectroscopic analysis. Additionally, the evolution law between the plasmon resonances and the luminescent enhancement as well as the emission spectrum of Eu3+ indicates a highly effective modulation of emission behavior by plasmons. This presents a novel strategy for enhancing the performance of optical micro- and nanodevices based on rare-earth ion materials, demonstrating significant potential in applications such as bioimaging and surface detection analysis.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"56 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143672629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative Fe(IV)-Triggered Chemiluminescence Assay for Rapid and Selective Detection of Total Phenolic Content 用于快速、选择性检测总酚类物质含量的创新型铁（IV）触发化学发光测定法

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-03-22 DOI: 10.1021/acs.analchem.5c00552

Huixin Shao, Chengyu Gu, Huajie Li, Ling Chen, Xiaohong Guan

{"title":"Innovative Fe(IV)-Triggered Chemiluminescence Assay for Rapid and Selective Detection of Total Phenolic Content","authors":"Huixin Shao, Chengyu Gu, Huajie Li, Ling Chen, Xiaohong Guan","doi":"10.1021/acs.analchem.5c00552","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00552","url":null,"abstract":"Total phenolic content reflecting the overall concentration of phenolics in water is a valuable indicator for evaluating water quality. However, current total phenolic content quantification technologies are unsatisfactory due to their complexity, time-consuming nature, limited reliability, and low selectivity. To overcome these problems, we utilized the high reactivity and selectivity of tetravalent iron (Fe(IV)) toward phenolics to develop a surrogate method for total phenolic content based on the quenching effect of phenolics on the chemiluminescence (CL) produced during the oxidation of naproxen (NAP) by Fe(IV) in the Fe(II)-activated periodate (Fe(II)/PI) process. Experimental results showed a strong linear relationship between the chemiluminescence quenching capacity (CLQC) values and total phenolic content in the Fe(II)/PI-NAP process. The high reactivity and superior selectivity of Fe(IV) toward phenolics enable rapid, highly sensitive, and robust anti-interference quantification of total phenolic content using the developed CL method. The limit of quantitation and limit of detection of the developed CL method for total phenolics determination were 1.34 and 0.40 μM, respectively, expressed as phenol equivalents. Finally, we validated the feasibility of using the CLQC value as a surrogate indicator for total phenolic content in various real water samples. This work introduces a novel method for quantifying total phenolic content by determining the CLQC value of water samples using the Fe(II)/PI-NAP process, offering a promising alternative for controlling the discharge of phenolics.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"70 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143672630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipophilicity Criteria for Ionic Components Used in Liquid-Junction-Free Reference Electrodes

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-03-21 DOI: 10.1021/acs.analchem.4c04156

Andrei D. Novakovskii, Vladimir V. Egorov, Danila V. Korostik

{"title":"Lipophilicity Criteria for Ionic Components Used in Liquid-Junction-Free Reference Electrodes","authors":"Andrei D. Novakovskii, Vladimir V. Egorov, Danila V. Korostik","doi":"10.1021/acs.analchem.4c04156","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c04156","url":null,"abstract":"Quantitative criteria for the lipophilicity of ionic components (ICs) used in PVC membranes of liquid-junction-free reference electrodes (LJFREs) providing a stable potential, independent of the electrolyte of the sample solution, have been formulated and theoretically substantiated. It is shown that the lipophilicity parameters of an ionic liquid (IL) or lipophilic salt (LS) as a whole, expressed in terms of partition constants, as well as the lipophilicities of individual ions, expressed in terms of single-ion partition coefficients, have both upper and lower limits. Therefore, a lipophilicity window for individual ions of IL or LS exists, and its width depends on the lipophilicity and concentration of the ions present in the sample solution. Besides that, upper lipophilicity limits for both an electrolyte as a whole and for its individual ions exist, beyond which any application of LJFREs is principally impossible under specified experimental conditions. Equations have been obtained that explicitly describe the upper (ULL) and lower limits of lipophilicity (LLL) of ionic components of LJFRE membranes as functions of the single-ion partition coefficients of electrolyte ions of the sample solution and their concentration, as well as the concentration of ionic components in the membrane phase and diffusion parameters. Predicted ULL and LLL values agree well with the corresponding values numerically calculated using a dynamic diffusion model and with experimental data. Some guides for the rational choice of IL or LS components satisfying specified experimental conditions are formulated.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"52 7 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143666752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatially Resolved Electrochemiluminescent Biosensor Combined with Nanopore Screening Devices for Simultaneous Determination of Deoxynivalenol and Aflatoxin B1

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-03-21 DOI: 10.1021/acs.analchem.5c00336

Jin-jin Wang, Xin-yan Zhang, Mei-fang Hua, Si-lun Li, Jie Wei, Qing-min Chen, Tian-hui Jiao, Yi Xu, Min Chen, Xiao-mei Chen

{"title":"Spatially Resolved Electrochemiluminescent Biosensor Combined with Nanopore Screening Devices for Simultaneous Determination of Deoxynivalenol and Aflatoxin B1","authors":"Jin-jin Wang, Xin-yan Zhang, Mei-fang Hua, Si-lun Li, Jie Wei, Qing-min Chen, Tian-hui Jiao, Yi Xu, Min Chen, Xiao-mei Chen","doi":"10.1021/acs.analchem.5c00336","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00336","url":null,"abstract":"Improving the preprocessing efficiency and creating multiple detection channels are crucial for building high-performance sensors. In this study, we developed a sensing platform that integrates a spatially resolved electrochemiluminescence (ECL) biosensor with a nanopore screening device for the simultaneous detection of deoxynivalenol (DON) and aflatoxin B1 (AFB1) in wheat. Zeolitic imidazolate framework-67(ZIF-67), which promotes the production of oxygen radicals (O2̇–), was integrated with luminol-capped silver nanoparticles (Luminol-AgNPs) to function as an ECL probe for DON detection. CON4H6–Ru, synthesized by covalently linking tris(4,4′-dicarboxylicacid-2,2′-bipyridyl) ruthenium(II) dichloride [Ru(dcbpy)3Cl2] and carbonyldiazide (CON4H6), was encapsulated in mesoporous silica nanoparticles (MSNs) and used as another ECL probe for the detection of AFB1. Subsequently, a reusable nanopore screening device was designed to eliminate macromolecular interference in the samples and improve the interference resistance of the sensing platform. Under the optimal experimental conditions, the sensing platform showed good linearity for DON concentrations from 0.005 to 150 μg/kg and AFB1 concentrations from 0.05 to 100 μg/kg, with detection limits of 1.80 × 10–4 and 1.09 × 10–3 μg/kg (S/N = 3), respectively.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"61 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143666756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rational Design of a New Class of Versatile Enzyme-Based Biosensors 基于酶的新型多功能生物传感器的合理设计

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-03-21 DOI: 10.1021/acs.analchem.4c06519

Shihao Pei, Sofie Dhondt, Samuel Babity, Davide Brambilla

{"title":"Rational Design of a New Class of Versatile Enzyme-Based Biosensors","authors":"Shihao Pei, Sofie Dhondt, Samuel Babity, Davide Brambilla","doi":"10.1021/acs.analchem.4c06519","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c06519","url":null,"abstract":"The majority of enzyme-based sensors rely on electrochemical approaches for continuous monitoring. For example, commercially available glucometers are electrochemical-based sensors. However, these sensors are not suitable for contactless monitoring as electron signals require direct conduction from the enzyme reaction site to the signal analyzing unit. Fluorescent dyes, on the other hand, emit photons that can penetrate certain barriers, making them ideal candidates for contactless monitoring. In this study, we investigated the design and functionality of a new class of biosensors based on the conjugation of enzymes with pH-sensitive fluorophores, creating a novel single-molecule biosensor capable of versatile, contactless detection of different disease- and treatment-related biomarkers. We conjugated various enzymes (glucose oxidase, phenylalanine ammonia-lyase, and β-lactamase) with pH-sensitive fluorophores (FITC and pH-sensitive Cy7 derivatives) and tuned linkers’ properties to modulate the distance between the enzyme and fluorophore, as well as the hydrophilicity of the linker. The experimental data demonstrate that fluorophore-conjugated enzymes exhibit substrate-dependent fluorescence responses under physiologically relevant buffered conditions, enabling the quantitative analysis of substrate concentrations through fluorescent signal detection. This innovative sensor design not only provides critical insights into enzyme-based fluorescent detection mechanisms but also represents a promising candidate for the development of next-generation contactless biosensing platforms.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"34 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143672631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selenenylsulfide Bond as a General Scaffold for Constructing Thiol Probes with Enhanced Response Rate 硒基硫化键作为构建硫醇探针的通用支架，可提高响应速度

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-03-21 DOI: 10.1021/acs.analchem.4c06526

Tao Ma, Jintao Zhao, Bingbing Chang, Meirong Yi, Jun Wu, Ruipeng Shen, Xinming Li, Baoxin Zhang, Jianguo Fang

{"title":"Selenenylsulfide Bond as a General Scaffold for Constructing Thiol Probes with Enhanced Response Rate","authors":"Tao Ma, Jintao Zhao, Bingbing Chang, Meirong Yi, Jun Wu, Ruipeng Shen, Xinming Li, Baoxin Zhang, Jianguo Fang","doi":"10.1021/acs.analchem.4c06526","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c06526","url":null,"abstract":"Leveraging disulfide (−S–S−) or diselenide (−Se–Se−) units as triggers in the design of small-molecule fluorescent probes for detecting intracellular reductive species has demonstrated high efficacy. However, selenenylsulfides, which exhibit reactivity between diselenides and disulfides, remain relatively unexplored. In this work, we compare the efficiency of −S–S–, seleno-sulfur (−Se–S−), and sulfur–selenium (−S–Se−) structural units in constructing thiol probes, disclose the scaffold of −Se–S– as a versatile recognition site for thiols, and successfully apply this unit to design a near-infrared (NIR) probe, ASC-SeS. The mechanism reveals that breaking the −Se–S– bond leads to a selenolate, which undergoes faster cyclization than the corresponding thiolate that is from the cleavage of the −S–S– or −S–Se– bond. Conjugation of this trigger unit with multiple NIR fluorophores validates the general applicability of linear selenenylsulfides in accelerating the response rate to thiols. By harnessing the superior thiol responsiveness of ASC-SeS, we employ this probe in live cells and in vivo, and elucidate a severe depletion of thiols in the drug-induced liver injury (DILI).","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"13 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143672632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Facile Method for Gel Electrophoresis with Intrinsic Fluorescence Imaging for Self-Aggregation and Stability Assay of Monoclonal Antibody

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-03-21 DOI: 10.1021/acs.analchem.5c00081

Zixian Yu, Yiren Cao, Lei Wang, Youli Tian, Rui Zou, Keer Chen, Weiwen Liu, Qiang Zhang, Qingjiang Wang, Baohong Zhang, Chengxi Cao

{"title":"A Facile Method for Gel Electrophoresis with Intrinsic Fluorescence Imaging for Self-Aggregation and Stability Assay of Monoclonal Antibody","authors":"Zixian Yu, Yiren Cao, Lei Wang, Youli Tian, Rui Zou, Keer Chen, Weiwen Liu, Qiang Zhang, Qingjiang Wang, Baohong Zhang, Chengxi Cao","doi":"10.1021/acs.analchem.5c00081","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00081","url":null,"abstract":"Monoclonal antibodies (mAbs) are widely used in tumor and autoimmune disease therapy and clinical diagnosis, but they suffer from self-aggregation, particularly dimer formation, impacting their stability, efficacy, and potentially causing severe allergies. Traditional methods for detecting mAb dimers, such as TEM, AUC, DLS, SEC, and CE, are limited by low throughput, high costs, and qualitative data, making them unsuitable for large-scale sample assays in biopharmaceuticals industry as well as antibody research. To address these issues, we developed a facile method of protein cross-linking gel electrophoresis with online intrinsic fluorescence imaging (PC-GE-IFI) for self-aggregation and stability assays of mAbs. This method enables the real-time quantification of monoclonal antibody (mAb) monomers and dimers with exceptional sensitivity, characterized by low detection limits (monomer: 0.9 nM; dimer: 0.45 nM) and a broad dynamic range (monomer: 2.50–2500 nM; dimer: 1.25–1250 nM). Furthermore, the sample wells can be used as windows for the assay of precipitates formed by mAb aggregation. The method supports accurate assessment of mAb dimerization and monomer purity under various stress conditions, including thermal stress, mechanical agitation, and freeze–thaw cycles. Moreover, the method allows concurrent analysis of dimers and precipitates across multiple samples at different concentrations, while nonlinear fitting provides the dissociation constant (Kd) for monomer–dimer interactions, a critical parameter that aids in assessing aggregation propensity, and informs the design and development of mAb products The PC-GE-IFI method has great potential for development, quality control, and safety assessment of mAbs, bispecific antibodies, ADCs, and protein drugs.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"214 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143666754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enrichment and Detection of HER2-Expressing Extracellular Vesicles Based on DNA Tetrahedral Nanostructures: A New Strategy for Liquid Biopsy in Breast Cancer

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-03-21 DOI: 10.1021/acs.analchem.4c06417

Feng Xu, Ke Wang, Chi Xu, Jingtong Xu, Chengjun Zhu, Ye Zhu, Chuandong Zhu, Wenwen Zhang, Jian Zhang, Zhe Li, Xiaoxiang Guan

{"title":"Enrichment and Detection of HER2-Expressing Extracellular Vesicles Based on DNA Tetrahedral Nanostructures: A New Strategy for Liquid Biopsy in Breast Cancer","authors":"Feng Xu, Ke Wang, Chi Xu, Jingtong Xu, Chengjun Zhu, Ye Zhu, Chuandong Zhu, Wenwen Zhang, Jian Zhang, Zhe Li, Xiaoxiang Guan","doi":"10.1021/acs.analchem.4c06417","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c06417","url":null,"abstract":"Extracellular vesicles (EVs) play a crucial role as important mediators of intercellular communication in the progression of tumors. The capture and analysis of tumor-derived EVs offer new possibilities for the application of cancer liquid biopsies. This study aims to construct a DNA tetrahedral nanostructure that specifically recognizes HER2 and CD63, enabling the effective enrichment and detection of HER2-expressing EVs (HEVs). We enriched HEVs from cell lines and 13 random clinical samples and validated their characteristics by dynamic light scattering, transmission electron microscopy, and Western blotting. Further, we detected HEVs levels in clinical samples. The HEVs levels in HER2-positive breast cancer patients were significantly higher than those in healthy/benign controls (mean, 4.737 vs 4.160 vs 4.144 U/μL, P < 0.0001), displaying a concentration gradient across different HER2 expression levels. This study establishes an approach for HEV detection, thus providing a new tool for the diagnosis of HER2-positive breast cancer.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"34 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143666753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Measurement of Total Ammonia Nitrogen and Free Ammonia via Integrated Electrochemical Acidification─Optode Flow Cell

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-03-21 DOI: 10.1021/acs.analchem.4c05994

Fabian Steininger, Theresa Merl, Niels Peter Revsbech, Klaus Koren

{"title":"Simultaneous Measurement of Total Ammonia Nitrogen and Free Ammonia via Integrated Electrochemical Acidification─Optode Flow Cell","authors":"Fabian Steininger, Theresa Merl, Niels Peter Revsbech, Klaus Koren","doi":"10.1021/acs.analchem.4c05994","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05994","url":null,"abstract":"The accurate measurement of total ammonia nitrogen (TAN) and free ammonia is crucial for various environmental, biomedical and industrial applications. We present a novel integrated system combining electrochemical water splitting with ammonia optodes to simultaneously measure TAN and free ammonia. Water electrolysis induces localized pH shifts, altering the ammonia speciation in the sample solution: an increase in pH near the cathode leads to conversion of NH4+ to NH3, enabling the measurement of TAN. Concurrently, a decrease in pH near the anode reduces the NH3 concentration to zero, enabling real-time zero calibration. In areas unaffected by these pH changes, the optode readout can effectively measure free NH3. The system demonstrates a measurement range of 0–300 mg·L–1 for both TAN and NH3, with a complete measurement cycle requiring only 6 min. The method was validated through the analysis of urine samples, showcasing its potential for real-time monitoring in clinical and environmental settings. The electrochemical speciation shifting allows for precise TAN measurement, while the zero-point calibration provided by the anode enhances the method’s robustness and reliability. Overall, this study introduces a versatile and efficient approach for the simultaneous determination of TAN and NH3, offering significant improvements in speed and operational simplicity.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"21 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143666762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0