Analytical Chemistry最新文献

{"title":"Introducing \"Identification Probability\" for Automated and Transferable Assessment of Metabolite Identification Confidence in Metabolomics and Related Studies.","authors":"Thomas O Metz, Christine H Chang, Vasuk Gautam, Afia Anjum, Siyang Tian, Fei Wang, Sean M Colby, Jamie R Nunez, Madison R Blumer, Arthur S Edison, Oliver Fiehn, Dean P Jones, Shuzhao Li, Edward T Morgan, Gary J Patti, Dylan H Ross, Madelyn R Shapiro, Antony J Williams, David S Wishart","doi":"10.1021/acs.analchem.4c04060","DOIUrl":"10.1021/acs.analchem.4c04060","url":null,"abstract":"<p><p>Methods for assessing compound identification confidence in metabolomics and related studies have been debated and actively researched for the past two decades. The earliest effort in 2007 focused primarily on mass spectrometry and nuclear magnetic resonance spectroscopy and resulted in four recommended levels of metabolite identification confidence─the Metabolite Standards Initiative (MSI) Levels. In 2014, the original MSI Levels were expanded to five levels (including two sublevels) to facilitate communication of compound identification confidence in high resolution mass spectrometry studies. Further refinement in identification levels have occurred, for example to accommodate use of ion mobility spectrometry in metabolomics workflows, and alternate approaches to communicate compound identification confidence also have been developed based on identification points schema. However, neither qualitative levels of identification confidence nor quantitative scoring systems address the degree of ambiguity in compound identifications in the context of the chemical space being considered. Neither are they easily automated nor transferable between analytical platforms. In this perspective, we propose that the metabolomics and related communities consider identification probability as an approach for automated and transferable assessment of compound identification and ambiguity in metabolomics and related studies. Identification probability is defined simply as 1/<i>N</i>, where <i>N</i> is the number of compounds in a database that matches an experimentally measured molecule within user-defined measurement precision(s), for example mass measurement or retention time accuracy, etc. We demonstrate the utility of identification probability in an <i>in silico</i> analysis of multiproperty reference libraries constructed from a subset of the Human Metabolome Database and computational property predictions, provide guidance to the community in transparent implementation of the concept, and invite the community to further evaluate this concept in parallel with their current preferred methods for assessing metabolite identification confidence.</p>","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":" ","pages":"1-11"},"PeriodicalIF":6.7,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11740175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tb-based Metal–Organic Framework-Referenced Fluorescence Assay for Distinguishing Hydroquinone from Its Isomers and Subsequent Quantitative Visual Detection of Cu2+","authors":"Hong Yu, Shuyi Liu, Jiatong Fan, Shuyun Zhu, Xian-En Zhao, Qian Liu","doi":"10.1021/acs.analchem.4c05616","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05616","url":null,"abstract":"Hydroquinone (HQ) and copper ions (Cu²⁺) are categorized as environmental pollutants that are severely limited in water. Designing a selective assay for discriminating HQ from its two isomers and the convenient determination of Cu²⁺ is of great importance. Herein, a Tb-based metal–organic framework (Tb-MOF) and HQ are assembled innovatively into a ratiometric fluorescence nanoprobe to selectively distinguish HQ and subsequent quantitative visual detection of Cu²⁺. The native blue emission of HQ at 338 nm is used as a response signal, while Tb-MOF with green fluorescence offers a reference signal at 545 nm. Notably, neither resorcinol (RC) nor catechol (CC) exhibits obvious emission under the same experimental conditions, which enables discriminating HQ from its isomers. Thus, a ratiometric fluorescence method has been designed for the selective detection of HQ with the fluorescence intensity ratio <i>F</i>₃₃₈/<i>F</i>₅₄₅ as the readout. The redox reaction between HQ and Cu²⁺ induces fluorescence quenching of HQ and no change to that of Tb-MOF, resulting in a noticeable color variation from blue-green to green via the naked eye. Furthermore, sensitive visual detection of Cu²⁺ is achieved with a low detection limit of 1.67 μM using a smartphone. The satisfactory recoveries and good repeatability of quantitative visualization determined in spiked water samples make this sensing platform suitable for on-site monitoring of environmental samples.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"28 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142975464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative Analysis of Hepatitis D Virus Using gRNA-Sensitive Semiconducting Polymer Dots","authors":"Ze Zhang, Yuyang Wu, Jinglun Xu, Zihui Meng, Qingmin Chen, Shengyan Yin","doi":"10.1021/acs.analchem.4c04147","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c04147","url":null,"abstract":"Hepatitis D virus (HDV) significantly influences the progression of liver diseases. Through clinical observations and database analyses, it has been established that patients coinfected with HDV and hepatitis B virus (HBV) experience accelerated progression toward cirrhosis, hepatocellular carcinoma (HCC), and liver failure compared to those infected solely with HBV. A higher viral load correlates with increased replicative activity, enhanced infectivity, and more severe disease manifestations. In this study, we use HDV gRNA-sensitive semiconducting polymer dots (Pdots) as the nanoprobes for the quantitative analysis of HDV copy number variations. The surface of the Pdots is engineered with a clamp design that includes a pair of reporter sequences, protection sequences, and capture sequences tailored to the conserved sequence length of the HDV genome. The capture sequence, comprising leading and trailing chains, specifically binds to the gRNA of the target virus. The protection sequence shields the Pdots from external interference, while the reporter sequence detects the presence of target gRNA through the degradation of fluorescent dye Cy5.5dt. We demonstrate the effectiveness of this assay in a stably transfected cell line derived from HepG2-HDV cells and its translational application in clinical samples from patients. Additionally, this nanobiosensor can accurately detect gRNA at femtomolar (fM) levels, a sensitivity unachievable by previously reported methods. This novel virus quantification system offers significant potential for clinical and virological applications, enhancing screening, early diagnosis, and personalized treatment strategies.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"22 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142975340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"APE1-Activated and NIR-II Photothermal-Enhanced Chemodynamic Therapy Guided by Amplified Fluorescence Imaging","authors":"Xiaofeng Bi, Jinyue Feng, Xiyuan Feng, Dianpeng Li, Yumin Wang, Shulin Zhao, Liangliang Zhang","doi":"10.1021/acs.analchem.4c05274","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05274","url":null,"abstract":"The development of intelligent nanotheranostic technology that integrates diagnostic and therapeutic functions holds great promise for personalized nanomedicine. However, most of the nanotheranostic agents exhibit “always-on” properties and do not involve an amplification step, which may largely limit imaging contrast and restrict therapeutic efficacy. Herein, we construct a novel nanotheranostic platform (Hemin/DHPs/PDA@CuS nanocomposite) by assembling DNA hairpin probes (DHPs) and hemin on the surface of PDA@CuS nanosheets that enables amplified fluorescence imaging and activatable chemodynamic therapy (CDT) of tumors. The cancer-relevant APE1 triggers nucleic acid amplification with DHPs to generate activatable and amplified fluorescence signals for discriminating cancer cells from normal cells. Meanwhile, excessive G-quadruplex/hemin-based DNAzyme are also activated, and they function as Fenton-like catalysts to catalyze the production of highly toxic hydroxyl radicals (•OH) for CDT. Moreover, owing to the excellent photothermal conversion efficiency in the near-infrared-II (NIR-II) window, the PDA@CuS not only improves the catalytic performance of CDT but also furnishes PTT. A remarkable antitumor therapeutic effect is demonstrated both <i>in vitro</i> and <i>in vivo</i>. Therefore, the Hemin/DHPs/PDA@CuS nanocomposite is expected to provide a promising avenue for precise imaging-guided antitumor therapy.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"41 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142975461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Directed Evolution of Multicyclic Peptides Using Yeast Display for Sensitive and Selective Fluorescent Analysis of CD28 on the Cell Surface","authors":"Chaoying Xu, Xiaoting Meng, Ping Chai, Hongtan Liu, Zengping Duan, Yu-Hsuan Tsai, Chuanliu Wu","doi":"10.1021/acs.analchem.4c05681","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05681","url":null,"abstract":"CD28 is a costimulatory receptor that provides the second signal necessary for T-cell activation and is associated with diseases, including rheumatoid arthritis, asthma, and cancer. Targeting CD28 is crucial for both functional bioanalysis and therapeutic development. Molecular probes, particularly fluorescent probes, can enhance our understanding of CD28′s cellular roles. However, existing antibody-based probes face challenges such as high production costs, low stability, and large size, which limit their bioanalytical applications. Thus, there is a need for smaller, robust probes that enable the sensitive and selective targeting of CD28. Multicyclic peptides have emerged as promising candidates for novel therapeutics and molecular probes. Recently, we identified disulfide-directed multicyclic peptides (DDMPs) that bind CD28 with submicromolar affinity; however, their relatively low affinity limits further applications. In this study, we develop a DDMP evolving system based on yeast display and error-prone PCR to identify high-affinity peptide binders. We obtained DDMPs with a picomolar affinity for CD28, exceptional binding specificity, and remarkable oxidative folding efficiency. Furthermore, we developed fluorescent probes and labeling strategies for detecting and visualizing CD28 expression in human T cells. This advancement opens new avenues for studying T-cell dynamics and activation states, which are essential for understanding immune responses and developing targeted therapies. Our study not only produces potent CD28 binders and probes but also establishes a robust platform for optimizing other multicyclic peptide-based probes and therapeutics.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"52 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142975468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colorimetric Xylenol Orange: A Long-Buried Aggregation-Induced Emission Dye and Restricted Rotation for Dual-Mode Sensing of pH and Metal Ions","authors":"Yu Li, Kechun Yu, Huihui Li, Shiyu Li, Jingxuan Han, Dong-Yu Guo, Shengming Chen, Qinhe Pan","doi":"10.1021/acs.analchem.4c05819","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05819","url":null,"abstract":"As the third largest class of dyes in the world, triphenylmethane dyes are widely applied in colorimetric sensing. However, triphenylmethane dyes are commonly nonfluorescent, which limits their sensing applications. It is worthwhile to study the fluorescence off/on control of triphenylmethane dyes and promote the applications of triphenylmethane dyes in sensing technology. In this work, the fluorescence off/on control was investigated by employing a triphenylmethane dye xylenol orange (XO), which is a colorimetric indicator for pH and metal ions. It was discovered that XO exhibited aggregation-induced emission (AIE), and thus, its fluorescence off/on was controlled by intramolecular rotation. This discovery broadens the optical properties of XO and transforms XO from a colorimetric dye to a colorimetric/fluorescent dual-mode AIE dye. It was further verified that the AIE-based fluorescence off/on control improved the sensing performance of XO. A bovine serum albumin-based rotation suppression method was applied to enhance the fluorescence emission of XO for colorimetric/fluorescent dual-mode indication of pH and metal ions. Compared with colorimetric sensing, colorimetric/fluorescent dual-mode sensing exhibits higher accuracy, ascribed to the self-validation effect. This work uncovers AIE-based fluorescence off/on control of triphenylmethane dyes and breathes new life into the sensing applications of triphenylmethane dyes.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"74 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142981255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Isolation of Quick-Response High-Affinity Aptamers for Continuous and Reagentless Detection of Thrombin","authors":"Yajing Gao, Ronghui Zhang, Qiao Na, Jing Li, Yi Zhang, Yu Zhang, Keyi Hu, Guangxin Zhang, Xin Zhang, Xinhui Lou","doi":"10.1021/acs.analchem.4c04808","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c04808","url":null,"abstract":"Continuous and reagentless biomolecular detection technologies are bringing an evolutionary influence on disease diagnostics and treatment. Aptamers are attractive as specific recognition probes because they are capable of regeneration without washing. Unfortunately, the affinity and dissociation kinetics of the aptamers developed to date show an inverse relationship, preventing continuous and reagentless detection of protein targets due to their low dissociation rates. Here, we describe an in vitro aptamer isolation strategy that enriches quick-response, high-affinity bivalent protein-binding aptamers. The method is general, as evidenced by the isolation of aptamers targeting thrombin and human serum albumin. We then demonstrated the excellent regeneration capability of the isolated thrombin aptamers using biolayer interferometry. The sensors instantly responded to alternating concentration changes of thrombin at nanomolar levels (200–500 nM), reaching highly consistent equilibrium signals within 10 s. In contrast, the well-known thrombin-binding aptamers, TBA-15 and TBA-29, were not capable of regeneration. Our study provides a simple means to obtain quick-response, high-affinity protein-binding aptamers. It can also be used for the isolation of aptamer pairs, which has been demonstrated to be quite challenging. Our study also provides insights into the rational design of aptamers to control their binding thermodynamics and kinetics.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"97 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142975460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cantilever-Enhanced Fiber-Optic Photoacoustic Spectrophone for Low-Pressure Gas Detection.","authors":"Chenxi Li, Xiao Han, Min Guo, Hongchao Qi, Jingya Zhang, Xinyu Zhao, Ke Chen","doi":"10.1021/acs.analchem.4c05422","DOIUrl":"10.1021/acs.analchem.4c05422","url":null,"abstract":"<p><p>A cantilever-enhanced fiber-optic photoacoustic (PA) spectrophone is reported for trace gas detection at a low-pressure environment. A cantilever-based fiber-optic Fabry-Perot (F-P) interferometer (FPI) is utilized for simultaneous measurement of air pressure and PA pressure. Since the cantilever resonance frequency follows air pressure linearly, the fundamental frequency intensity modulation (1<i>f</i>-IM) technique is applied to scan the frequency response of the solid PA signal from tube wall absorption for tracking the cantilever resonance frequency in real time. The second-harmonic wavelength modulation spectroscopy (2<i>f</i>-WMS) technique is used to measure the gas PA pressure wave at the cantilever resonance. According to the inverse restriction relationship of air pressure on the PA excitation and cantilever detection, the measured gas PA signal at the low-pressure environment is enhanced. The target gas concentration is corrected by the measured air pressure, which makes the spectrophone generally applicable under any pressure. The experimental results indicate that the normalized noise equivalent absorption (NNEA) coefficients of the spectrophone in the standard atmospheric pressure and the low-pressure environment of 60 kPa are 2.2 × 10^-9 and 2.0 × 10^-9 cm^-1·W·Hz^-1/2, respectively. 0.1 ppm acetylene (C₂H₂) can be detected at any air pressure. The detected maximum relative error of 10 ppm C₂H₂ gas under different pressures is less than ±9% and the error is reduced to less than ±2% when the concentration rises to 70 ppm. In the pressure range of 60-100 kPa, the cantilever-enhanced fiber-optic PA spectrophone has extremely high accuracy and pressure stability, covering the pressure range of most ground gas detection scenes.</p>","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":" ","pages":"838-847"},"PeriodicalIF":6.7,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smart and Noninvasive SERS Immunosensors for Monitoring Dynamic Expression of Cytokines during Cell Pyroptosis","authors":"Chenyu Zhang, Zutao Chen, Guohua Qi, Yu Tian, Xiongjian Zheng, Xingkang Diao, Jiao Kong, Xingkai Ju, Jing Li, Shaojun Dong, Yongdong Jin","doi":"10.1021/acs.analchem.4c05539","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05539","url":null,"abstract":"Accompanying the occurrence of inflammatory reaction to release cytokines, pyroptosis can activate an immune response for resistance against cancer. Consequently, elevated levels of cytokines released by cancer cells are highly correlated with the effectiveness of cancer treatment. Herein, a noninvasive surface-enhanced Raman spectroscopy (SERS) immunosensor was developed to sensitively and specifically measure the tumor necrosis factor-α (TNF-α), a proinflammatory cytokine, during the cell pyroptosis process. The sandwiched structure of the sensor is functionalized with a TNF-α binding antibody for detecting TNF-α at concentrations as low as 1 pg/mL. Importantly, electrical stimulation (ES) can fleetly trigger cancer cell pyroptosis to induce the overexpression of receptor interacting protein 3 (RIP3), which is a significant protein that regulates the inflammatory response. The overexpression of RIP3 can activate caspase-1 to promote the upregulation of cytokine levels. Notably, the cytokine levels of TNF-α released from cancer cells (MCF-7 cells) were apparently higher than those of normal cells (MCF-10A cells) during pyroptosis detected by the SERS immunosensors. Due to its obvious superiorities of simple fabrication and fast readout without sample pretreatment, the developed SERS platform has a potential application value for diagnosis and treatment of cancer.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"43 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142981261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DACH-Based Chiral Sensing Platforms as Tunable Benzamide-Chiral Solvating Agents for NMR Enantioselective Discrimination","authors":"Shuai-Hua Shi, Xiao-Juan Wang, Yuan-Yuan Gao, Tao Wang, Chun-Yu Wang, Zi Wang, Qiang Wang, Fei Li, Gao-Wei Li","doi":"10.1021/acs.analchem.4c06024","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c06024","url":null,"abstract":"Chiral discrimination is an indispensable tool that has pivotal importance in the assignment of absolute configuration and determination of enantiomeric excess in chiral compounds. A series of enantiomerically pure <i>trans</i>-1,2-diaminocyclohexane (<i>trans</i>-DACH)-derived benzamides were first synthesized by simple chemical steps, and 14 variated derivatives <b>6a–6n</b> have been assessed as NMR chiral solvating agents (CSAs) for discrimination of the signals of mandelic acid (MA) in ¹H NMR analysis. The highly efficient chiral recognition of CSA <b>6e</b> on different substrates, including MAs, carboxylic acids, amino acid derivatives, and phosphoric acids (32 examples), was expanded via ¹H, ¹⁹F, and ³¹P NMR spectroscopy. The quality of enantiodiscrimination was evaluated by means of the enantioresolution parameter <i>R</i>_s. Single-crystal X-ray analysis of three derivatives <b>6c</b>, <b>6e</b>, and <b>6h</b> helped to understand enantiomeric recognition for the promising NMR analysis. Interestingly, the NMR signals of nonequivalent protons between the <i>R</i> and <i>S</i> configurations were completely opposite in the presence of CSA <b>6e</b> and its stereoisomer, which can be utilized to establish a straightforward method for the configuration assignment of diverse hydroxy acid substrates.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"1 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142981256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}