Analytical Chemistry最新文献_第10页

Target-Responsive Regulation of Bacteria-Surface Magnetic Element for Self-Powered Analysis of Aflatoxin B1 in Microbial Fuel Cell 微生物燃料电池中黄曲霉毒素B1自供电分析中细菌表面磁元件的靶响应调控

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-04-13 DOI: 10.1021/acs.analchem.5c00463

Yuxin Wang, Jiale Sun, Cui Wang, Lingbo Qu, Lin Zhang, Yapiao Li, Rong-Bin Song, Zhaohui Li

{"title":"Target-Responsive Regulation of Bacteria-Surface Magnetic Element for Self-Powered Analysis of Aflatoxin B1 in Microbial Fuel Cell","authors":"Yuxin Wang, Jiale Sun, Cui Wang, Lingbo Qu, Lin Zhang, Yapiao Li, Rong-Bin Song, Zhaohui Li","doi":"10.1021/acs.analchem.5c00463","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00463","url":null,"abstract":"The limitation of the sensing mode greatly restricts the detectable species and detection specificity of microbial fuel cell-based self-powered biosensors (MFC-SPBs). Herein, we develop a bacterial quantity change-based sensing mode for MFC-SPBs, in which the Fe3O4@Au content modified on exoelectrogenic bacteria is designed to correlate with analyte concentration for regulating the bacterial numbers absorbed onto the magnetic auxiliary anode. The polydopamine and Au nanoparticles comodified bacteria are attached with complementary DNA for hybridization with aptamer-modified Fe3O4@Au nanospheres. When aflatoxin B1 (AFB1) is used as the model analyte, its appearance can cause the liberation of Fe3O4@Au nanospheres from bacteria due to aptamer recognition. Furthermore, introduced exonuclease I can achieve a recycling amplification effect, intensifying the release of Fe3O4@Au nanospheres. With the decrease in bacteria-surface Fe3O4 content, bacteria that can be adsorbed onto the anode in a magnetic field will be reduced, leading to a decrease in the performance of MFC-SPBs. The results show that the developed MFC-SPBs can quantitatively determine AFB1 with a limit of detection of 5 nM (S/N = 3). Also, the MFC-SPBs show good detection specificity and can assess AFB1 in peanut samples. Considering the good specificity and species diversity of aptamers, we believe that this developed sensing mode will receive wide attention in the field of MFC-SPBs.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"37 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143827537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leaching Kinetics of Iron-Rich Alkali-Activated Materials under Sulfuric Acid Attack: An In Situ Method Using Low-Field NMR Relaxometry 硫酸侵蚀下富铁碱活化物质的浸出动力学：一种原位低场核磁共振弛豫法

IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-04-13 DOI: 10.1021/acs.analchem.4c0544910.1021/acs.analchem.4c05449

Ziyou Yu, Rodrigo de Oliveira-Silva, Everton Lucas Oliveira, Nana Wen, Yiannis Pontikes and Dimitrios Sakellariou*,

{"title":"Leaching Kinetics of Iron-Rich Alkali-Activated Materials under Sulfuric Acid Attack: An In Situ Method Using Low-Field NMR Relaxometry","authors":"Ziyou Yu, Rodrigo de Oliveira-Silva, Everton Lucas Oliveira, Nana Wen, Yiannis Pontikes and Dimitrios Sakellariou*, ","doi":"10.1021/acs.analchem.4c0544910.1021/acs.analchem.4c05449","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05449https://doi.org/10.1021/acs.analchem.4c05449","url":null,"abstract":"Proton nuclear magnetic resonance (NMR) relaxometry is applied to monitor the temporal changes of Fe3+ ion concentration in an aqueous solution by exploiting the paramagnetic behavior of Fe3+ ions. The nondestructive and noninvasive nature of NMR techniques allows us to observe in situ the leaching behavior of iron-rich alkali-activated materials (AAMs) in sulfuric acid solution. By calibrating the relation between proton relaxation rates and Fe3+ ion concentrations, we can quantitatively measure the real-time release of Fe3+ ions into the acid solution. Traditional methods for estimating the acid resistance of AAMs are ex situ, often resulting in additional error and being time consuming. The proposed in situ NMR method is a novel and efficient complementary technique to traditional methods for studying the kinetics of acid attack on iron-rich AAMs. With the high temporal resolution of NMR measurements, the kinetics of Fe3+ release can be described by a proposed model that considers the sample’s geometry. Two iron-rich AAM samples with different calcium molar ratios are examined. It is found that in the “low Ca content” range (<20 mol %) AAM with higher calcium content has a lower apparent reaction rate constant, indicating greater resistance to sulfuric acid attack.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"97 15","pages":"8241–8247 8241–8247"},"PeriodicalIF":6.7,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143854096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Target-Responsive Regulation of Bacteria-Surface Magnetic Element for Self-Powered Analysis of Aflatoxin B1 in Microbial Fuel Cell 微生物燃料电池中黄曲霉毒素B1自供电分析中细菌表面磁元件的靶响应调控

IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-04-13 DOI: 10.1021/acs.analchem.5c0046310.1021/acs.analchem.5c00463

Yuxin Wang, Jiale Sun, Cui Wang, Lingbo Qu, Lin Zhang*, Yapiao Li*, Rong-Bin Song* and Zhaohui Li,

{"title":"Target-Responsive Regulation of Bacteria-Surface Magnetic Element for Self-Powered Analysis of Aflatoxin B1 in Microbial Fuel Cell","authors":"Yuxin Wang, Jiale Sun, Cui Wang, Lingbo Qu, Lin Zhang*, Yapiao Li*, Rong-Bin Song* and Zhaohui Li, ","doi":"10.1021/acs.analchem.5c0046310.1021/acs.analchem.5c00463","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00463https://doi.org/10.1021/acs.analchem.5c00463","url":null,"abstract":"The limitation of the sensing mode greatly restricts the detectable species and detection specificity of microbial fuel cell-based self-powered biosensors (MFC-SPBs). Herein, we develop a bacterial quantity change-based sensing mode for MFC-SPBs, in which the Fe3O4@Au content modified on exoelectrogenic bacteria is designed to correlate with analyte concentration for regulating the bacterial numbers absorbed onto the magnetic auxiliary anode. The polydopamine and Au nanoparticles comodified bacteria are attached with complementary DNA for hybridization with aptamer-modified Fe3O4@Au nanospheres. When aflatoxin B1 (AFB1) is used as the model analyte, its appearance can cause the liberation of Fe3O4@Au nanospheres from bacteria due to aptamer recognition. Furthermore, introduced exonuclease I can achieve a recycling amplification effect, intensifying the release of Fe3O4@Au nanospheres. With the decrease in bacteria-surface Fe3O4 content, bacteria that can be adsorbed onto the anode in a magnetic field will be reduced, leading to a decrease in the performance of MFC-SPBs. The results show that the developed MFC-SPBs can quantitatively determine AFB1 with a limit of detection of 5 nM (S/N = 3). Also, the MFC-SPBs show good detection specificity and can assess AFB1 in peanut samples. Considering the good specificity and species diversity of aptamers, we believe that this developed sensing mode will receive wide attention in the field of MFC-SPBs.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"97 15","pages":"8484–8490 8484–8490"},"PeriodicalIF":6.7,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143854121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New Atomic Mass Tags for Enhanced Multiplexing Capability of Multiplexed Ion Beam Imaging Time-of-Flight (MIBI-TOF) Analysis 用于增强多路复用离子束成像飞行时间（MIBI-TOF）分析多路复用能力的新型原子质量标签

IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-04-13 DOI: 10.1021/acs.analchem.4c0430010.1021/acs.analchem.4c04300

Rashmi Kumar, Felix J. Hartmann, Patricia Favaro, Daniel Ho, Trevor Bruce, Mako Goldston, Angie Spence, Erin F. McCaffrey, Sean C. Bendall* and Michael Angelo*,

{"title":"New Atomic Mass Tags for Enhanced Multiplexing Capability of Multiplexed Ion Beam Imaging Time-of-Flight (MIBI-TOF) Analysis","authors":"Rashmi Kumar, Felix J. Hartmann, Patricia Favaro, Daniel Ho, Trevor Bruce, Mako Goldston, Angie Spence, Erin F. McCaffrey, Sean C. Bendall* and Michael Angelo*, ","doi":"10.1021/acs.analchem.4c0430010.1021/acs.analchem.4c04300","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c04300https://doi.org/10.1021/acs.analchem.4c04300","url":null,"abstract":"Antibodies conjugated to metal chelating polymers are routinely used in high-dimensional multiplexed single cell mass spectrometric imaging techniques, such as immunohistochemistry-based multiplexed ion beam imaging by time-of-flight (MIBI-TOF) mass spectrometry, imaging mass cytometry (IMC), and flow cytometry-based CyTOF. However, successful multiplexed capability of these techniques is heavily dependent on the stability of the metal-chelates used. Chelate stability is governed by the ionic radius of the metal used, which in some cases can fall below or exceed the optimal range for commercially available DTPA-based polymers. In this study, we have developed and optimized macrocyclic chelators for metals with relatively small (i.e., Ga) or large (i.e., Tl) atomic radii. In agreement with previously published studies, we observed NOTA to be a suitable chelator for Ga, whereas DOTA was found to be an ideal chelator for Tl and larger lanthanides, such as La, Ce, and Pr. DOTA and DTPA chelator dendrimers were synthesized and conjugated to primary antibodies that were subsequently used for tissue staining. Antibodies conjugated with the DOTA-dendrimer were more stable and exhibited more specific staining than those modified with the corresponding DTPA-dendrimer. With these new chelates, we incorporated seven new reporter channels into a highly multiplexed MIBI-TOF imaging study containing 44 protein epitope markers on various tissues. To the best of our knowledge, this is the largest multiplexed panel used to date for MIBI-TOF applications.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"97 15","pages":"8220–8230 8220–8230"},"PeriodicalIF":6.7,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143854063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leaching Kinetics of Iron-Rich Alkali-Activated Materials under Sulfuric Acid Attack: An In Situ Method Using Low-Field NMR Relaxometry 硫酸侵蚀下富铁碱活化物质的浸出动力学：一种原位低场核磁共振弛豫法

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-04-13 DOI: 10.1021/acs.analchem.4c05449

Ziyou Yu, Rodrigo de Oliveira-Silva, Everton Lucas Oliveira, Nana Wen, Yiannis Pontikes, Dimitrios Sakellariou

{"title":"Leaching Kinetics of Iron-Rich Alkali-Activated Materials under Sulfuric Acid Attack: An In Situ Method Using Low-Field NMR Relaxometry","authors":"Ziyou Yu, Rodrigo de Oliveira-Silva, Everton Lucas Oliveira, Nana Wen, Yiannis Pontikes, Dimitrios Sakellariou","doi":"10.1021/acs.analchem.4c05449","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05449","url":null,"abstract":"Proton nuclear magnetic resonance (NMR) relaxometry is applied to monitor the temporal changes of Fe3+ ion concentration in an aqueous solution by exploiting the paramagnetic behavior of Fe3+ ions. The nondestructive and noninvasive nature of NMR techniques allows us to observe in situ the leaching behavior of iron-rich alkali-activated materials (AAMs) in sulfuric acid solution. By calibrating the relation between proton relaxation rates and Fe3+ ion concentrations, we can quantitatively measure the real-time release of Fe3+ ions into the acid solution. Traditional methods for estimating the acid resistance of AAMs are ex situ, often resulting in additional error and being time consuming. The proposed in situ NMR method is a novel and efficient complementary technique to traditional methods for studying the kinetics of acid attack on iron-rich AAMs. With the high temporal resolution of NMR measurements, the kinetics of Fe3+ release can be described by a proposed model that considers the sample’s geometry. Two iron-rich AAM samples with different calcium molar ratios are examined. It is found that in the “low Ca content” range (<20 mol %) AAM with higher calcium content has a lower apparent reaction rate constant, indicating greater resistance to sulfuric acid attack.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"13 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143827531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

T5 Exonuclease Improves the Identification of Aptamers Specific for Small Molecules from the Early Screening Library T5核酸外切酶提高了早期筛选文库中小分子核酸适体的特异性鉴定

IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-04-13 DOI: 10.1021/acs.analchem.5c0025110.1021/acs.analchem.5c00251

Shixin Liu, Jiaxin Cheng, Xinyue Zhang, Yanlin Qin, Yongshu Li, Huan Liu, Qin Wu, Jianjun Hou and Xixia Liu*,

{"title":"T5 Exonuclease Improves the Identification of Aptamers Specific for Small Molecules from the Early Screening Library","authors":"Shixin Liu, Jiaxin Cheng, Xinyue Zhang, Yanlin Qin, Yongshu Li, Huan Liu, Qin Wu, Jianjun Hou and Xixia Liu*, ","doi":"10.1021/acs.analchem.5c0025110.1021/acs.analchem.5c00251","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00251https://doi.org/10.1021/acs.analchem.5c00251","url":null,"abstract":"Aptamers are usually obtained by the SELEX process; however, it is time-consuming to perform repetitive work. Therefore, a new method was designed to rapidly obtain high-affinity aptamers from an early screening library. Target-binding aptamers were isolated from early library based on the function of T5 exonuclease (Exo), which could digest nonaffinity aptamers and retain the complete sequence of affinity aptamers. Meanwhile, enzymatic electrophoresis, label-free dye displacement assay, q-PCR, and isothermal titration calorimetry were used to characterize aptamer affinity. The ninth ssDNA library (9R) of 5-hydroxymethylfurfural (5-HMF) was digested by T5 Exo, and high-throughput sequencing was performed on the digested library (E-9R). 20 sequences with the highest multiplicity were selected for characterization. The results showed that there were 8 affinity aptamers in the general screening library and 15 in the digested library, which were always more than in the general screening library when the target concentration decreased. Subsequently, the aptamers from enzymatic-electrophoresis experiments were characterized by the label-free dye displacement assay and q-PCR to distinguish the binding strength to 5-HMF. It was found that 9R-e1 from E-9R exhibited the best binding affinity, with the Kd value of 8.8 μM. The same enzymatic conditions were used for the patulin (PAT) screening library to verify this method’s universal applicability. Similarly, affinity aptamers were always more abundant in the enzymatic library, and an aptamer with a Kd value of 6.40 μM was found in PAT-E6R. The enzymatic method ensures high-affinity aptamer availability while reducing the number of selection rounds, significantly shortening the selection time and reducing the cost.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"97 15","pages":"8444–8451 8444–8451"},"PeriodicalIF":6.7,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143854067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mass Determination of Filled and Empty AAV5 Particles Enabled by Nanoelectrospray Ionization and Proton Transfer Charge Reduction 利用纳米电喷雾电离和质子转移电荷还原测定填充和空AAV5粒子的质量

IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-04-12 DOI: 10.1021/acs.analchem.5c0109510.1021/acs.analchem.5c01095

Nicolas J. Pizzala, Boukar K.S. Faye, Hangtian Song, Li Tao and Scott A. McLuckey*,

{"title":"Mass Determination of Filled and Empty AAV5 Particles Enabled by Nanoelectrospray Ionization and Proton Transfer Charge Reduction","authors":"Nicolas J. Pizzala, Boukar K.S. Faye, Hangtian Song, Li Tao and Scott A. McLuckey*, ","doi":"10.1021/acs.analchem.5c0109510.1021/acs.analchem.5c01095","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01095https://doi.org/10.1021/acs.analchem.5c01095","url":null,"abstract":"The mass determination of bio-ions that exceed a megadalton poses many challenges. While it is possible to generate gaseous ions from large biocomplexes, including intact viruses, via nanoelectrospray ionization (nESI), generating mass information using conventional ensemble measurements (i.e., from conventional mass spectra) requires the resolution of charge states. As biocomplexes increase in size, overlap of adjacent charge states becomes increasingly problematic. Single ion measurements that enable the simultaneous determination of mass/charge and charge can overcome the charge state overlap problem. However, ensemble measurements are, in principle, much faster. We demonstrate here the mass determination of empty and filled adeno-associated virus particles, serotype 5 (AAV5), both separately and as a mixture using nESI, gas-phase proton transfer ion/ion reactions, and time-of-flight mass analysis. The ion/ion reactions are used to reduce charge states to the point at which they can be resolved, and UniDec, a publicly available deconvolution program, is used to facilitate mass determination. This work demonstrates that mass measurements of binary mixtures of empty and filled AAV5 particles as large as 3.7–4.5 MDa can be enabled via the use of single proton transfer ion/ion reactions to facilitate charge state resolution.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"97 15","pages":"8189–8194 8189–8194"},"PeriodicalIF":6.7,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mass Determination of Filled and Empty AAV5 Particles Enabled by Nanoelectrospray Ionization and Proton Transfer Charge Reduction 利用纳米电喷雾离子化和质子转移电荷还原法测定填充和空 AAV5 粒子的质量

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-04-12 DOI: 10.1021/acs.analchem.5c01095

Nicolas J. Pizzala, Boukar K.S. Faye, Hangtian Song, Li Tao, Scott A. McLuckey

{"title":"Mass Determination of Filled and Empty AAV5 Particles Enabled by Nanoelectrospray Ionization and Proton Transfer Charge Reduction","authors":"Nicolas J. Pizzala, Boukar K.S. Faye, Hangtian Song, Li Tao, Scott A. McLuckey","doi":"10.1021/acs.analchem.5c01095","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01095","url":null,"abstract":"The mass determination of bio-ions that exceed a megadalton poses many challenges. While it is possible to generate gaseous ions from large biocomplexes, including intact viruses, via nanoelectrospray ionization (nESI), generating mass information using conventional ensemble measurements (i.e., from conventional mass spectra) requires the resolution of charge states. As biocomplexes increase in size, overlap of adjacent charge states becomes increasingly problematic. Single ion measurements that enable the simultaneous determination of mass/charge and charge can overcome the charge state overlap problem. However, ensemble measurements are, in principle, much faster. We demonstrate here the mass determination of empty and filled adeno-associated virus particles, serotype 5 (AAV5), both separately and as a mixture using nESI, gas-phase proton transfer ion/ion reactions, and time-of-flight mass analysis. The ion/ion reactions are used to reduce charge states to the point at which they can be resolved, and UniDec, a publicly available deconvolution program, is used to facilitate mass determination. This work demonstrates that mass measurements of binary mixtures of empty and filled AAV5 particles as large as 3.7–4.5 MDa can be enabled via the use of single proton transfer ion/ion reactions to facilitate charge state resolution.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"119 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endogenous Enzyme-Activated Spatial Confinement DNA Nanowire with a Tumor Cell-Specific Response for High-Precision Imaging of the Tumor/Normal Cells Boundary 内源性酶激活的空间约束DNA纳米线具有肿瘤细胞特异性反应，用于肿瘤/正常细胞边界的高精度成像

IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-04-12 DOI: 10.1021/acs.analchem.5c0016210.1021/acs.analchem.5c00162

Lei Wang, Tingting Zhao, Congkai Wang, Xiaohan Xu, Wang Yao, Xiaozhe Pang, Shenghao Xu* and Xiliang Luo*,

{"title":"Endogenous Enzyme-Activated Spatial Confinement DNA Nanowire with a Tumor Cell-Specific Response for High-Precision Imaging of the Tumor/Normal Cells Boundary","authors":"Lei Wang, Tingting Zhao, Congkai Wang, Xiaohan Xu, Wang Yao, Xiaozhe Pang, Shenghao Xu* and Xiliang Luo*, ","doi":"10.1021/acs.analchem.5c0016210.1021/acs.analchem.5c00162","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00162https://doi.org/10.1021/acs.analchem.5c00162","url":null,"abstract":"Developing tumor cell-specific imaging approaches is essential for the clear delineation of tumor margins. However, traditional imaging approaches suffered from low reaction kinetics as well as limited tumor specificity resulting from their “always active” sensing mode, making it difficult to accurately depict tumor boundary. To address these limitations, we developed an endogenous enzyme-activated spatial confinement DNA nanowire probe (E-SCNW) with an enhanced tumor/normal cell discrimination ratio for high precision imaging of the tumor/normal cells boundary. The spatial confinement effect can improve reaction kinetics, and the endogenous enzyme-activation design can confine fluorescence response to the tumor cells region. Additionally, no additional cell delivery carriers were required during the cross of the cell membrane into the intracellular space. It is worth noting that benefiting from the spatial confinement effect and endogenous enzyme-activation design, the detection limit was decreased by nearly 25.6-fold and the tumor/normal cells discrimination ratio was enhanced by nearly 4.46-fold through using E-SCNW, indicating promising prospects in high-precision imaging of the tumor/normal cells boundary.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"97 15","pages":"8429–8435 8429–8435"},"PeriodicalIF":6.7,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endogenous Enzyme-Activated Spatial Confinement DNA Nanowire with a Tumor Cell-Specific Response for High-Precision Imaging of the Tumor/Normal Cells Boundary 内源性酶激活的空间约束DNA纳米线具有肿瘤细胞特异性反应，用于肿瘤/正常细胞边界的高精度成像

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-04-12 DOI: 10.1021/acs.analchem.5c00162

Lei Wang, Tingting Zhao, Congkai Wang, Xiaohan Xu, Wang Yao, Xiaozhe Pang, Shenghao Xu, Xiliang Luo

{"title":"Endogenous Enzyme-Activated Spatial Confinement DNA Nanowire with a Tumor Cell-Specific Response for High-Precision Imaging of the Tumor/Normal Cells Boundary","authors":"Lei Wang, Tingting Zhao, Congkai Wang, Xiaohan Xu, Wang Yao, Xiaozhe Pang, Shenghao Xu, Xiliang Luo","doi":"10.1021/acs.analchem.5c00162","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00162","url":null,"abstract":"Developing tumor cell-specific imaging approaches is essential for the clear delineation of tumor margins. However, traditional imaging approaches suffered from low reaction kinetics as well as limited tumor specificity resulting from their “always active” sensing mode, making it difficult to accurately depict tumor boundary. To address these limitations, we developed an endogenous enzyme-activated spatial confinement DNA nanowire probe (E-SCNW) with an enhanced tumor/normal cell discrimination ratio for high precision imaging of the tumor/normal cells boundary. The spatial confinement effect can improve reaction kinetics, and the endogenous enzyme-activation design can confine fluorescence response to the tumor cells region. Additionally, no additional cell delivery carriers were required during the cross of the cell membrane into the intracellular space. It is worth noting that benefiting from the spatial confinement effect and endogenous enzyme-activation design, the detection limit was decreased by nearly 25.6-fold and the tumor/normal cells discrimination ratio was enhanced by nearly 4.46-fold through using E-SCNW, indicating promising prospects in high-precision imaging of the tumor/normal cells boundary.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"20 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143822515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0