Analytical Chemistry最新文献_第10页

IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-07-22

Jinyan Li, Lingling Guo, Hua Kuang, Chuanlai Xu and Xinxin Xu*,

引用次数: 0

IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-07-22

Shuangli Wu, Zhixuan Yu, Jianguo Zhao, Yuchen Wang, Jianli Nan, Lili Xu, Xiaoqi Li, Ling Yang and Shaojun Dong*,

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IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-07-22

Xiaohong Hu, Huan Luo, Nahoko Kasai*, Hizuru Nakajima, Shungo Kato, Katsumi Uchiyama and Sifeng Mao*,

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IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-07-22

Ruben Szabó, Melinda Andrási and Attila Gáspár*,

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IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-07-22

Lukas Kopecky, Caroline J. Sands, María Gómez-Romero, Shivani Misra, Elizabeth J. Want and Timothy M. D. Ebbels*,

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IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-07-22

Huiying Xu, Yujing Zhang, Lihua Shen, Shu Yu, Changqing Li, Xianfei Zeng*, Qin Liu* and Bang-Ce Ye*,

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Cellular and Intravital ATP Imaging Using a Merocyanine-Hybrided Rhodamine Fluorescent Probe. 细胞和活体ATP成像使用的merocyanine - hybridrhodamine荧光探针。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-07-22 DOI: 10.1021/acs.analchem.5c02198

Zuo-Hu Chai,Gaowei Pan,Fujian Qi,Yu Zhang,Fang Dai,Shengxiang Zhang,Bo Zhou

{"title":"Cellular and Intravital ATP Imaging Using a Merocyanine-Hybrided Rhodamine Fluorescent Probe.","authors":"Zuo-Hu Chai,Gaowei Pan,Fujian Qi,Yu Zhang,Fang Dai,Shengxiang Zhang,Bo Zhou","doi":"10.1021/acs.analchem.5c02198","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c02198","url":null,"abstract":"The real-time intravital imaging of adenosine triphosphate (ATP) is essential for comprehending the intricate relationship between energy metabolism and cellular functions. To develop highly effective molecular tools for intravital ATP imaging, herein we designed a merocyanine-hybrided rhodamine fluorescent probe named PCR. The advantages offered by this probe toward ATP include near-infrared excitation/emission (λex/λem = 700/758 nm), a rapid (<3 min) and reversible response, an ideal dissociation constant (Kd = 7.05 mM), a relatively low detection limit (36.9 μM), deep tissue penetration (180 μm), and two-photon excitability. With the aid of the probe, we successfully detected both exogenous and endogenous ATP generation in viable cells. Furthermore, we monitored dynamic fluctuations in ATP levels under various metabolic perturbations and observed an augmented ATP production in cancer cells relative to normal cells. Subsequently, our investigation unveiled that β-lapachone, a well-established anticancer agent, induces cancer cell death by triggering reactive oxygen species-dependent energy crisis. Lastly, we visualized oscillations in ATP levels during cerebral ischemia/reperfusion by examining HT-22 cells and live mouse brain. The aforementioned successful application showcases the probe's potential for further elucidating ATP function in intricate biological environments, particularly in vivo.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"25 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144684306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of Co/Ce Dual Active Sites Single-Atom Cerium Nanozyme with the Synergistic Effect and Peroxidase-like Activity for Total Antioxidant Capacity Evaluation. 具有协同作用的Co/Ce双活性位点单原子铈纳米酶的合成及过氧化物酶样活性评价

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-07-22 DOI: 10.1021/acs.analchem.5c01219

Linghui Guo,Huiyun Zhang,Zigeng Zhao,Shouting Zhang,Wenping Hu

{"title":"Synthesis of Co/Ce Dual Active Sites Single-Atom Cerium Nanozyme with the Synergistic Effect and Peroxidase-like Activity for Total Antioxidant Capacity Evaluation.","authors":"Linghui Guo,Huiyun Zhang,Zigeng Zhao,Shouting Zhang,Wenping Hu","doi":"10.1021/acs.analchem.5c01219","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01219","url":null,"abstract":"The design and modulation of the catalytic activity of nanozymes are critical for advancing their development. In this study, a novel Ce-based single-atom nanozyme with Co/Ce dual-metal active sites (named Co-Ce@NC) was successfully synthesized. Co-Ce@NC exhibits excellent peroxidase-like (POD) activity, demonstrating both enhanced affinity and an accelerated reaction rate for H2O2. This may be attributed to the high single-atom loading (Ce: 9.61 wt %; Co: 0.46 wt %) caused by surface defects of the material and the synergistic effect between Co and Ce, which enhance the Ce3+/Ce4+ ratio and thereby rapidly trigger the production of reactive oxygen species (ROS) from H2O2. Based on the competition between ascorbic acid (AA) and ROS during the oxidation of TMB, a colorimetric platform was developed to detect the total antioxidant capacity (TAC). This platform was applied for detecting TAC in commercial beverages and fruits, indicating its significant potential for evaluating antioxidant quality in food. This study not only introduces a novel approach for enhancing the performance of Ce-based single-atom nanozymes but also emphasizes their broad applicability in TAC detection.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"16 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144684312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Collisional Cross-Section Prediction for Multiconformational Peptide Ions with IM2Deep. 用IM2Deep预测多肽离子的碰撞截面。

IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-07-22 Epub Date: 2025-07-08 DOI: 10.1021/acs.analchem.5c01142

Robbe Devreese, Alireza Nameni, Arthur Declercq, Emmy Terryn, Ralf Gabriels, Francis Impens, Kris Gevaert, Lennart Martens, Robbin Bouwmeester

引用次数: 0

In Vivo Identification and Spatial Distribution of Crown Gall Disease Biomarkers in Grapevine. 葡萄冠瘿病生物标志物的体内鉴定及空间分布。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-07-22 DOI: 10.1021/acs.analchem.5c02019

Trong Nguyen-Huu,Nina Ogrinc,Léa Ledoux,Cédric Jacquard,Isabelle Kerzaon,Céline Lavire,Christophe Clément,Michel Salzet,Ludovic Vial,Lisa Sanchez,Isabelle Fournier

{"title":"In Vivo Identification and Spatial Distribution of Crown Gall Disease Biomarkers in Grapevine.","authors":"Trong Nguyen-Huu,Nina Ogrinc,Léa Ledoux,Cédric Jacquard,Isabelle Kerzaon,Céline Lavire,Christophe Clément,Michel Salzet,Ludovic Vial,Lisa Sanchez,Isabelle Fournier","doi":"10.1021/acs.analchem.5c02019","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c02019","url":null,"abstract":"Grapevine crown gall is caused by the tumorigenic bacterium Allorhizobium vitis. The fight against this disease is a major challenge in viticulture since this pathogen can systemically colonize the plant host, persisting in vineyards once infected. Understanding the dynamics of the plant-pathogen relationship not only improves our knowledge of plant biology but also could lead to innovative strategies for sustainable agricultural practices. In this way, this study aimed to gain insights into metabolic changes occurring in the tumor induced by A. vitis, using two techniques, Water-Assisted Laser Desorption/Ionization Mass Spectrometry (WALDI) and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging (MALDI MSI). We highlighted compounds such as phenolics alongside specific lipids acting as stress signaling regulators. Moreover, vitopine, produced after plant cell transformation by A. vitis and exclusively found in infected plants, was successfully identified by real time detection by SpiderMass and localized for the first time through MSI. We illustrate here the effectiveness of SpiderMass and MSI in identifying biomarkers of grapevine crown gall, enhancing our understanding of plant responses to bacterial infection. These techniques allow for real-time analysis and spatial distribution mapping of metabolites, paving the way for future research and potential applications in diagnostics.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"110 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144678244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0