Analytical Chemistry最新文献

{"title":"Ultrasensitive Quantification of Poloxamer 188 in Adeno-Associated Virus Products by All-Ion Fragmentation MS/MS","authors":"Sisi Zhang,Jing Wang,Hui Xiao,Ning Li","doi":"10.1021/acs.analchem.6c03755","DOIUrl":"https://doi.org/10.1021/acs.analchem.6c03755","url":null,"abstract":"Abstract Poloxamer 188 (P188; Pluronic F68) is a nonionic surfactant frequently used in biotherapeutics, particularly in adeno-associated virus (AAV) formulations, in which it is present at trace concentrations (0.001%−0.005%). However, its accurate quantification is difficult, because of its low abundance, frequently limited sample volumes, and the ionization variability of its PEO−PPO−PEO triblock. Here, we developed an all-ion fragmentation (AIF) workflow with 44 Da and 58 Da neutral-loss-triggered data dependent (dd)-MS2 that fragments all precursors and performs quantification via the polyethylene glycol (PEG) diagnostic ion (m/z 133.1), thereby decreasing spectral complexity and adduct variability. In contrast to the most sensitive reported method, LC−MRM, using a 20 μL injection volume, which achieved a dynamic range of 250−10,000 ng/mL (0.000025%−0.001%), our method achieved a 0.00002% LLOQ with 1 μL injections, thus providing an ∼20-fold sensitivity gain that enables replicate analysis and a dynamic range 2 orders of magnitude wider (0.00002%−0.1%). Our assay is vendor-, formulation-, and LC-buffer-agnostic, and achieves ±10% accuracy and ∼10% CVs across AAV-relevant levels. Significant P188 losses attributable to container adsorption were observed; incorporation of 50% acetonitrile restored recovery and is recommended for sample preparation.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"39 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlabelled Spatial Metabolic Covariance Analysis (US-MCA) Reveals Tumor-Associated Reorganization of Interorgan Choline Metabolic Response Patterns in Lung Cancer","authors":"Xiaoyu Fan,Xiangyi Wang,Rong Yan,Yiran Zhao,Hongtao Jin,Jiuming He","doi":"10.1021/acs.analchem.6c03732","DOIUrl":"https://doi.org/10.1021/acs.analchem.6c03732","url":null,"abstract":"Abstract Tumor progression is increasingly recognized as a systemic disease that extends beyond local metabolic reprogramming and alters the metabolism of host organs. However, existing metabolomics approaches either lack spatial information or provide predominantly static abundance measurements, limiting assessment of coordinated, organ-specific metabolic responses in vivo. We combined whole-body airflow-assisted desorption electrospray ionization mass spectrometry (AFADESI-MSI) with a semi-quantitative, perturbation-based framework termed Unlabelled Spatial Metabolic Covariance Analysis (US-MCA) to characterize tumor-associated spatiotemporal covariation in metabolic response patterns across organs. Whole-body AFADESI-MSI depicted organ-specific metabolic profiles under physiological conditions and revealed heterogeneous metabolic alterations in host organs during tumor growth. Comparison with the D9-choline tracing experiment showed concordant response patterns for the subset of reliably detected choline-derived signals, while highlighting the limited isotopic coverage of downstream one-carbon metabolites. US-MCA indicated tumor-associated reorganization of choline-related response patterns across multiple organs. Coordinated changes in choline- and methionine-related signals in the tumor and liver were consistent with a potential association between hepatic metabolic adaptation and tumor metabolic demand. Cell co-culture experiments further showed methionine dependence of lung cancer cells under the tested conditions, but did not directly provide inter-organ metabolite transport.This study established US-MCA as an exploratory framework for assessing semi-quantitative, spatially resolved metabolic covariation after a defined perturbation. The results support tumor-associated reorganization of choline-related metabolic response patterns, but do not constitute measurements of biochemical mass flux, instantaneous reaction rates, or direct inter-organ metabolite transport.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"39 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorinated Microporous Organic Network-Based Cooling-Assisted SPME for Ultra-Trace Determination of Fluorinated Liquid Crystal Monomers","authors":"Jiayu Chen,Yehong Han,Fengxia Qiao,Junfa Yin,Dandan Han,Hongyuan Yan","doi":"10.1021/acs.analchem.6c04012","DOIUrl":"https://doi.org/10.1021/acs.analchem.6c04012","url":null,"abstract":"Abstract Fluorinated liquid crystal monomers (FLCMs), widely used in electronic displays, have emerged as persistent and bioaccumulative contaminants, yet their ultra-trace determination in complex matrices remains challenging. Herein, a cooling-assisted solid-phase microextraction (CA-SPME) method based on a fluorinated microporous organic network (F-MON) coating, coupled with GC−MS/MS, is developed for ultra-trace determination of FLCMs in environmental water and soil. The F-MON coating, featuring abundant fluorinated active sites and a hierarchical porous structure, provides strong affinity toward FLCMs via synergistic fluorine−fluorine, hydrophobic, and π−π interactions. A self-designed cooling-assisted device establishes a temperature gradient that suppresses analyte desorption and enhances partitioning into the extraction phase. The method exhibits rapid adsorption kinetics and multilayer adsorption behavior. Under optimized conditions, ultralow limits of quantification (0.02−0.26 pg mL−1) and high enrichment factors (6394−15782) are achieved, with excellent linearity (r ≥ 0.9995), good reproducibility (RSDs ≤ 11.5%), and negligible matrix effects (−22.1% to 20.7%). Compared with existing approaches, this method offers reduced sample and solvent consumption, simplified operation, and superior sensitivity. This work provides a robust analytical platform for trace FLCMs and highlights the synergistic potential of fluorinated porous fiber materials and cooling-assisted extraction strategies in advanced sample pretreatment.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"37 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-Particle ICP−MS Enabled Multiplex Digital Detection of Nucleic Acids via Feedback Recombinase Polymerase Amplification","authors":"Feng Dai,Man He,Guolin Yuan,Zewei Cui,Ting Guo,Beibei Chen,Bin Hu","doi":"10.1021/acs.analchem.6c05515","DOIUrl":"https://doi.org/10.1021/acs.analchem.6c05515","url":null,"abstract":"Abstract Digital droplet assays, with their single-molecule sensitivity, hold great promise for early disease diagnosis by enabling precise biomarker quantification and the generation of patient-specific molecular profiles. Inductively coupled plasma mass spectrometry (ICP−MS) has shown its great potential in the quantification of biomolecules with elemental labeling strategies. However, the development of ICP−MS-based digital assays has been limited, primarily due to the challenge of efficiently anchoring elemental labels within droplets. To address this, we developed a feedback recombinase polymerase amplification (F-RPA)-based digital analyzer via a facile droplet generator and combined it with single particle (sp)-ICP−MS for the quantitative analysis of human papillomavirus (HPV) genetic markers. In this system, HPV DNA molecules are randomly partitioned across tens of thousands of agarose droplets, following a Poisson distribution. Individual encapsulated target DNA molecules initiate on-demand F-RPA amplification and anchoring of elemental tags for subsequent signal transduction. Leveraging the discrete event counting characteristics and multi-element analytical capabilities of sp-ICP−MS, the digital F-RPA assay achieved highly sensitive and multiplexed analysis, with detection limits as low as 20 copies/μL for HPV16 and 9 copies/μL for HPV18 DNA. The method was validated by quantifying the HPV DNA load in cervical cancer cell lines and further applied to clinical cervical swab samples, where it identified 26 HPV16-positive and 20 HPV18-positive cases with 100% precision. This digital F-RPA sp-ICP−MS system emerges as a robust diagnostic platform for trace biomarker analysis, significantly advancing the role of sp-ICP−MS in digital droplet detection and clinical diagnosis.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"13 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Multiple Signal Amplification Strategy from the Cascade Reactions of Catalytic Hairpin Assembly and Hybridization Chain Reaction for a Novel Bioelectronic Sensor","authors":"Junyong Lou,Jiajun Zhan,Futing Wang,Yujin Li,Yifan Lyu,Hongfen Yang,Dan Yang,Ren Cai,Weihong Tan","doi":"10.1021/acs.analchem.6c03093","DOIUrl":"https://doi.org/10.1021/acs.analchem.6c03093","url":null,"abstract":"Abstract A highly sensitive bioelectronic sensor (BES) was developed to analyze miRNA-182, utilizing Ag@Au hexagonal nanoplates (Ag@Au HNPs) in conjunction with catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR) cascade reactions. The Ag@Au HNPs improve the conductivity between glucose oxidase (GOD) and carbon paper (CP), thereby contributing to enhanced electron transport in the BES. In addition, the multi-amplification strategy of the BES integrates the CHA-HCR cascade reaction with a capacitor to efficiently amplify the detection signal and enhance the sensitivity. As a result, the BES enables highly sensitive detection of miRNA-182 with a low limit of detection (LOD) of 0.34 fM (S/N = 3) in a wide linear response range of 1∼104 fM. Furthermore, the BES exhibited significant applicability in the detection of miRNA-182 in real samples.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"57 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Performance 19F-Tags in Detecting Protein “Hot Spots” in Live Mammalian Cells","authors":"Chao-Yu Cui,Xia-Yan Li,Mo-Han Li,Yu-Hao Xiao,Xun-Cheng Su","doi":"10.1021/acs.analchem.6c02561","DOIUrl":"https://doi.org/10.1021/acs.analchem.6c02561","url":null,"abstract":"Abstract The understanding of weak protein interactions in native intracellular environments remains challenging due to macromolecular crowding, transient associations, and the highly heterogeneous chemical and spatial landscape of live cells. In-cell 19F NMR has been shown to be a promising tool for the characterization of intracellular interactions and dynamics of proteins, but the quality and sensitivity of 19F-reporters in detecting intracellular protein interactions remain largely unexplored. Here, we systematically designed and synthesized ten 19F-tags (T1−T10) with diverse hydrophobicity and rigidity. The 19F-tag was site-specifically installed in ubiquitin via thiol conjugation chemistry. We then examined the protein−19F-tag conjugates across various crowding media including chemical reagents, proteins, and cell lysates. Our findings reveal that the chemical structure of 19F-tags is critical for accurately reflecting the intrinsic behavior of the target protein. Unexpectedly, certain 19F-tags themselves, despite their small sizes, tend to interact transiently with commonly used crowding media (such as BSA), thereby confounding authentic protein interactions. Crucially, the live-cell NMR assay demonstrated that the 19F-tag T8 (3-chloro-2-(phenylsulfonyl)-5-(trifluoromethyl)pyridine) (PSPy-m3F), featuring a shorter linker tether and a balanced amphiphilic profile, serves as an optimal 19F NMR reporter. It effectively resolves ubiquitin “hot spots” that are highly sensitive to weak intracellular interactions, providing a useful high-resolution tool for in-cell structural biology.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"11 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isotope-Coded Click Chemistry-Enabled Enrichment for Reliable Profiling and Quantification of Carboxyl Metabolites","authors":"Xin Tao,Min Ren,Tao Zhang,Hou-Kai Li,Jian-Bo Wan","doi":"10.1021/acs.analchem.6c05924","DOIUrl":"https://doi.org/10.1021/acs.analchem.6c05924","url":null,"abstract":"Abstract Carboxyl-containing metabolites are involved in diverse biological processes and are closely associated with metabolic diseases. However, their sensitive and reliable profiling by liquid chromatography−mass spectrometry (LC−MS) remains challenging because of low endogenous abundance, inefficient electrospray ionization, and substantial interference from complex biological matrices. Here, we developed a click chemistry-enabled enrichment (CCEE) strategy that integrates isotope-coded derivatization with chemoselective enrichment for sensitive and selective profiling and quantification of carboxyl-containing metabolites in biological samples. CCEE markedly improved detection sensitivity and chromatographic performance while effectively reducing matrix interference. The method also exhibited good linearity, reproducibility, and quantitative accuracy. Application of CCEE to human serum enabled broad profiling of the carboxyl submetabolome and revealed metabolic alterations associated with metabolic dysfunction-associated fatty liver disease (MAFLD). Targeted absolute quantification of representative metabolites further validated the observed alterations. Notably, serum bilirubin levels were significantly lower in individuals with MAFLD and strongly correlated with multiple clinical indicators. Overall, CCEE provides a sensitive and selective strategy for profiling and quantifying the carboxyl submetabolome, facilitating the investigation of metabolic dysregulation and the discovery of candidate biomarkers for metabolic diseases.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"13 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Two-Step Strategy Based on Fpg Recognition and RCA-CRISPR/Cas12a for Ultrasensitive Detection of Gene Mutation","authors":"Shiying Zhou,Tingting Xiang,Zengqin Cha,Jiayan Song,Yuanfang Xiang,Xiang Li,Chunlan Huang,Qibing Mei,Xinwu Huang,Chunxiang Zhang,Jianming Wu","doi":"10.1021/acs.analchem.6c04145","DOIUrl":"https://doi.org/10.1021/acs.analchem.6c04145","url":null,"abstract":"Abstract Accurate detection of gene mutations is crucial for acute myeloid leukaemia (AML) diagnosis and treatment. Although CRISPR/Cas systems enable sensitive mutation detection, their adaptability is constrained by dependence on restriction endonucleases. Herein, we propose a two-step strategy, termed Fpg-RCA-CRISPR/Cas12a for ultrasensitive detection of the p53 R175H, which integrates formamidopyrimidine DNA glycosylase (Fpg)-mediated recognition with rolling circle amplification (RCA)-coupled CRISPR/Cas12a technology. Validation confirmed that Fpg exhibits minimal activity when adenine (A) is opposite the damaged site. Based on this finding, we designed a padlock probe with a site-specific 8-oxoguanine (8-oxoG) modification. This modification enables selective recognition of G > A mutations. For the target TP53 c.524G > A (p53 R175H), the G > A substitution opposite the 8-oxoG site blocks Fpg cleavage, facilitating probe circularization and subsequent RCA. The resulting RCA products then activate the trans-cleavage activity of Cas12a, enabling cascade signal amplification and generating a strong fluorescence output. For wild-type p53, Fpg-mediated cleavage prevents circularization and amplification, yielding weak fluorescence. Taking advantage of the high specificity of the Fpg enzyme and the cascade amplification effect of the RCA−CRISPR/Cas12a system, this method achieves a limit of detection (LOD) of 0.29 fM and covers a linear range from 0.8 fM to 800 pM, along with high reproducibility, excellent selectivity, and negligible background interference. Notably, the assay can detect mutation frequencies as low as 0.1% in excess wild-type p53. Crucially, its clinical feasibility was confirmed by analyzing human serum samples, yielding results consistent with standard assays and thus demonstrating its practical reliability and translational potential.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"19 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasensitive and Specific Detection of Neutralization-Correlated Serum Antibodies Via Secondary Antibody−DNA Linker-Mediated Magnetic Bead−Based Proximity Extension Assay","authors":"Long Zhao,Yujue Wang,Lin Xiao,Shiyu Cao,Wei Wang,Feng Ye","doi":"10.1021/acs.analchem.6c03485","DOIUrl":"https://doi.org/10.1021/acs.analchem.6c03485","url":null,"abstract":"Abstract Sensitive, accurate, and readily deployable detection of neutralizing antibody (NAb) titers is essential for evaluating vaccine efficacy and population-level immunity. Here, we report an Antibody-DNA Linker (AND Linker) comprising a fusion of Protein A/G, a G4S flexible peptide, and duck circovirus replication initiator protein (DCV). This design enables orthogonal, site-specific assembly: the N-terminal Protein A/G binds the IgG Fc region, while the C-terminal DCV covalently captures 5′ ssDNA at a defined 1:1 molar ratio. Unlike random chemical crosslinking, which suffers from heterogeneous attachment sites, variable stoichiometry, and poor reproducibility, the AND Linker ensures uniform functional orientation and minimal batch-to-batch variation. By optimizing ssDNA secondary structure, we further enhanced DCV recognition efficiency. Integrating this linker into a magnetic bead-based proximity extension assay (termed SADL-MPEA), we achieved femtomolar-level detection of serum antibodies highly correlated with neutralizing activity. For HPV16 and HPV18, SADL-MPEA demonstrated limits of detection (LOD) approximately three orders of magnitude lower than conventional PEA using random chemical conjugation. In clinical serum validation against the pseudovirus neutralization test (PBNT), SADL-MPEA showed strong concordance (Pearson’s r = 0.84−0.85), confirming its utility as a reliable surrogate for functional neutralization capacity. Diagnostic performance was exceptional, with sensitivities of 90.9% (HPV16) and 83.3% (HPV18), 100% specificity for both, and an area under the ROC curve of 0.99. Crucially, SADL-MPEA requires only antigen-coated magnetic beads to adapt to new targets, as the DNA-labeled secondary antibody remains universal. This platform offers a simple, high-throughput, and scalable solution for NAb-correlated antibody detection in research and clinical settings.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"1 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photobleaching-Driven Ratiometric Fluorescent Switching of ZGO:Mn Nanorods/RhB for Iodine Vapor Monitoring with Integrated Gas-Phase Separation","authors":"Xiaohan Jin,Yue Gao,Jian Zhang,Zhu Jin,Yuanhang Ming,Lianhang Li,Lifang He,Kui Zhang","doi":"10.1021/acs.analchem.6c04851","DOIUrl":"https://doi.org/10.1021/acs.analchem.6c04851","url":null,"abstract":"Abstract Conventional fluorescent sensors generally rely on complex, time-consuming structural engineering of recognition moieties. By contrast, leveraging photobleaching for eco-friendly, reagent-free signal transduction has long been impeded by irreversible fluorophore degradation. Additionally, direct liquid-phase detection of trace radioactive iodine species (I2 or I−) persistently suffers from severe matrix interference. Herein, we engineered a novel photobleaching-driven ratiometric fluorescent platform that exploits the marked difference in photostability between structurally robust manganese-doped zinc gallate nanorods (ZGO:Mn NRs) and photo-stable rhodamine B (RhB) fluorophores. Under low-power UV irradiation, photogenerated superoxide radicals and holes induce pronounced self-quenching of ZGO:Mn NRs without compromising their crystalline structure. Upon exposure to I2, these reactive species are rapidly scavenged, restoring the intrinsic green luminescence of the nanorods and producing a distinct orange-to-green color transition. The ratiometric probe exhibits excellent sensitivity, with a limit of detection (LOD) as low as 1.54 nM. To eliminate matrix interference, we seamlessly integrate a custom 3D-printed miniaturized gas-phase separation module, which converts nonvolatile I− into volatile I2, with a smartphone-based machine-vision readout system. The system was successfully applied to detect trace iodide in complex soil, raw lake water, and kelp extract samples, with recoveries ranging from 94.7% to 105.6%, demonstrating great promise for nuclear regulatory monitoring.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"50 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}