{"title":"In Vivo Identification and Spatial Distribution of Crown Gall Disease Biomarkers in Grapevine.","authors":"Trong Nguyen-Huu,Nina Ogrinc,Léa Ledoux,Cédric Jacquard,Isabelle Kerzaon,Céline Lavire,Christophe Clément,Michel Salzet,Ludovic Vial,Lisa Sanchez,Isabelle Fournier","doi":"10.1021/acs.analchem.5c02019","DOIUrl":null,"url":null,"abstract":"Grapevine crown gall is caused by the tumorigenic bacterium Allorhizobium vitis. The fight against this disease is a major challenge in viticulture since this pathogen can systemically colonize the plant host, persisting in vineyards once infected. Understanding the dynamics of the plant-pathogen relationship not only improves our knowledge of plant biology but also could lead to innovative strategies for sustainable agricultural practices. In this way, this study aimed to gain insights into metabolic changes occurring in the tumor induced by A. vitis, using two techniques, Water-Assisted Laser Desorption/Ionization Mass Spectrometry (WALDI) and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging (MALDI MSI). We highlighted compounds such as phenolics alongside specific lipids acting as stress signaling regulators. Moreover, vitopine, produced after plant cell transformation by A. vitis and exclusively found in infected plants, was successfully identified by real time detection by SpiderMass and localized for the first time through MSI. We illustrate here the effectiveness of SpiderMass and MSI in identifying biomarkers of grapevine crown gall, enhancing our understanding of plant responses to bacterial infection. These techniques allow for real-time analysis and spatial distribution mapping of metabolites, paving the way for future research and potential applications in diagnostics.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"110 1","pages":""},"PeriodicalIF":6.7000,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acs.analchem.5c02019","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Grapevine crown gall is caused by the tumorigenic bacterium Allorhizobium vitis. The fight against this disease is a major challenge in viticulture since this pathogen can systemically colonize the plant host, persisting in vineyards once infected. Understanding the dynamics of the plant-pathogen relationship not only improves our knowledge of plant biology but also could lead to innovative strategies for sustainable agricultural practices. In this way, this study aimed to gain insights into metabolic changes occurring in the tumor induced by A. vitis, using two techniques, Water-Assisted Laser Desorption/Ionization Mass Spectrometry (WALDI) and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging (MALDI MSI). We highlighted compounds such as phenolics alongside specific lipids acting as stress signaling regulators. Moreover, vitopine, produced after plant cell transformation by A. vitis and exclusively found in infected plants, was successfully identified by real time detection by SpiderMass and localized for the first time through MSI. We illustrate here the effectiveness of SpiderMass and MSI in identifying biomarkers of grapevine crown gall, enhancing our understanding of plant responses to bacterial infection. These techniques allow for real-time analysis and spatial distribution mapping of metabolites, paving the way for future research and potential applications in diagnostics.
期刊介绍:
Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.