Analytical Chemistry最新文献

{"title":"Mapping of Amyloid-β Aggregates In Vivo by a Fluorescent Probe with Dual Recognition Moieties","authors":"Fanghui Liang, Lijun Ma, Guoyang Zhang, Jiahao Han, Mingguang Zhu, Chaofeng Zhu, Yulong Jin, Zhuo Wang","doi":"10.1021/acs.analchem.4c06385","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c06385","url":null,"abstract":"The spontaneous aggregation of amyloid-β (Aβ) leads to neuronal cell death in the brain and causes the development of Alzheimer’s disease (AD). The efficient detection of the aggregation state of Aβ holds significant promise for the early diagnosis and subsequent treatment of this neurodegenerative disorder. Currently, most of the fluorescent probes used for the detection of Aβ fibrils share similar recognition moieties, such as the <i>N</i>,<i>N</i>-dimethylamino group, <i>N</i>,<i>N</i>-diethylamino group, and piperidyl group. The development of fluorescent probes incorporating novel recognition groups will, in principle, bring new properties for the detection and imaging of Aβ aggregates. Herein, we designed and synthesized three fluorescent probes (<b>1</b> CarbCN, <b>2</b> NTCN, and <b>3</b> NCarbCN) based on dicyanoisophorone. The probe <b>3</b> NCarbCN, which integrated two recognition moieties, a carbamate unit (a new recognition group) together with a <i>N</i>,<i>N</i>-dimethylamino group, showed a sensitive turn-on fluorescence response toward the early aggregation state of Aβ, along with a high binding affinity (<i>K</i>_d = 59 nM) and recognition selectivity for Aβ fibrils. Theoretical calculations revealed that the carbamate unit of <b>3</b> NCarbCN could provide an additional three hydrogen bonding interaction with Aβ₄₂ fibrils. Furthermore, the probe <b>3</b> NCarbCN efficiently crossed the blood–brain barrier and exhibited a higher response in the brains of AD model mice. Co-staining of isolated brain sections with monoclonal antibody further demonstrated specific binding of <b>3</b> NCarbCN to Aβ plaques in the brains of AD model mice, thus demonstrating its great potential for the early diagnosis of such neurodegenerative disorders.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"24 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Situ Analysis of Plant Tissue Using Arc iKnife Ionization Mass Spectrometry","authors":"Wen Cai, Yuanji Gao, Changsheng Shi, Hongyue An, Xingyu Li, Danqun Huo, Siqi Chen, Yi He, Kaineng Huang, Yuanting Zhu, Chen Xiao, Qi Li, Jing Zhang, Yong Zuo, Dongliang Li","doi":"10.1021/acs.analchem.4c05128","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05128","url":null,"abstract":"This study developed a portable arc iKnife ionization mass spectrometry (AII-MS) technique integrating a surgical knife with low-temperature arc plasma to interact with plant tissues. The thermal energy from the arc plasma induces the sputtering of water-containing plant tissues, leading to the formation of aerosols. These aerosols are then charged by plasma-generated ions, producing charged microdroplets that are ultimately detected by a mass spectrometer. AII-MS effectively mitigates the challenges of aerosol or tissue charring associated with arc ionization. Moreover, appropriate nitrogen cooling minimizes surface damage to plant samples, while the carrier gas facilitates the efficient generation and transfer of aerosols. Comparative analyses conducted before and after tissue cutting with a surgical knife revealed that this technology is well-suited for examining various fresh and dried plant tissues including seeds, fruits, leaves, roots, stems, flowers, and bark. The characteristic components were identified under both positive and negative ion modes. Notably, potassium nitrate was detected in various plant samples for the first time, which may be attributed to its extensive use as a nutrient in vegetable cultivation. In summary, the developed AII-MS can effectively be used for plant tissue analysis, demonstrating high throughput, environmental sustainability, rapid processing, and reliability.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"24 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-Time 5-Hydroxyindoleacetic Acid Monitoring in Guinea Pig Brain Using a Molecular Imprinted Polymer-Based Galvanic Redox Potentiometric Sensor","authors":"Yuxin Peng, Fenghui Zhu, Huan Wei, Jiping Ni, Xin Yang, Yichun Yao, Ying Jiang","doi":"10.1021/acs.analchem.4c04990","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c04990","url":null,"abstract":"5-Hydroxyindoleacetic acid (5-HIAA), a vital metabolite of serotonin (5-HT), is crucial for understanding metabolic pathways and is implicated in various mental disorders. In situ monitoring of 5-HIAA is challenging due to the lack of affinity ligands and issues with electrochemical fouling. We present an advanced sensing approach that integrates customizable molecular imprinting polymer (MIP) with self-driven galvanic redox potentiometry (GRP) for precise, real-time in vivo monitoring of 5-HIAA. The sensor, featuring pyrrole as the functional monomer in the MIP on the micrometer-sized bipolar carbon fiber electrodes, exhibited nanomolar sensitivity and superior selectivity for 5-HIAA over biosynthetic pathway analogs like 5-hydroxytryptophan (5-HTP) and serotonin. The _MIPGRP sensor demonstrated excellent reversibility and resistance to fouling, enabling continuous monitoring in live guinea pig brains. We observed that intraperitoneal 5-HTP injection increases brain 5-HIAA levels, which is amplified up to 8-fold with Carbidopa pretreatment, providing deeper insights into the serotonergic signaling pathway. This work underscores the _MIPGRP sensor’s potential as a versatile and reliable tool for advancing neuroscience research.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"120 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Droplet Microfluidic Sensor for Point-of-Care Measurement of Plasma/Serum Total Free Thiol Concentrations","authors":"Liam Carter, Adrian Nightingale, Martin Feelisch, Xize Niu","doi":"10.1021/acs.analchem.4c04163","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c04163","url":null,"abstract":"Total free thiols are an important marker of the whole-body redox state, which has been shown to be associated with clinical outcome in health and disease. Recent investigations have suggested that increased insight may be gained by monitoring alterations of redox state in response to exercise and hypoxia and to monitor redox trajectories in disease settings. However, conducting such studies is challenging due to the requirement for repeated venous blood sampling and intensive lab work. Droplet microfluidic sensors offer an alternative platform for developing a point-of-care testing approach using small sample volumes and automated systems to complement or ultimately replace laboratory testing. Here we developed a small, portable droplet microfluidic sensor that can measure total free thiol concentrations in 20 μL human plasma (or serum) samples, providing a reading in less than 10 min. This system features a novel method to enhance the mixing of reagent and analyte in droplets containing viscous biological fluids. The results in a range of real-world human plasma samples showed equivalence with current standard laboratory assays while reducing sample volume requirements 9-fold and fully automating the process. Micro hematocrit capillaries allowed testing of capillary blood samples collected by fingerprick lancing. The system was used to monitor total free thiols using fingerprick samples in healthy volunteers and revealed significant changes in total free thiols in response to food intake and exercise. This device has the potential to improve our ability to conduct physiological studies of total free thiol level changes and improve our understanding of redox physiology, which may ultimately be applied in redox medicine to improve patient care.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"34 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual Ionic Signal Detection: Modulation of Surface Charge of Nanofluidic Iontronics by Dual-Split Gate Voltages","authors":"Xiaoqing Wu, Yajie Chen, Xinmeng Wang, Zhixiao Si, Qiujiao Du, Pengcheng Gao","doi":"10.1021/acs.analchem.4c03696","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c03696","url":null,"abstract":"Nanofluidic iontronics, including the field-effect ionic diode (FE-ID) and field-effect ionic transistor (FE-IT), represent emerging nanofluidic logic devices that have been employed in sensitive analyses. Making analyte recognitions in predefined nanofluidic devices has been verified to improve the sensitivity and selectivity using a single ionic signal, such as ionic current amplification, rectification, and Coulomb blockade. However, the detection of analytes in complex systems generally necessitates more diverse signals beyond just ionic currents. Here, we demonstrated that dual ionic signals, steady ionic switching ratio, and transient response time (<i>t</i>_s) act as detection signals modulated by dual-split gate voltages along the nanochannel for the detection of charged analytes. With an increase in gate voltage, the switching ratio decreases in both FE-ID and FE-IT, whereas the response time exhibits an exponential increase specifically in the FE-ID. Moreover, the response time shows no significant correlation with the external transmembrane voltage in the FE-IT. These results contribute to the optimization of reconfigurable iontronics through gate voltage modulation, providing a theoretical foundation for multiple ionic signal detection.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"119 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Label-Free and Sequence-Independent Isothermal Amplification Strategy for the Simultaneous Detection of Genomic 5-Methylcytosine and 5-Hydroxymethylcytosine","authors":"Feng Zhang, Chengpeng Li, Di Yang, Bingqian Liu, Yue Zhou, Zhixu Zhou, Hang Zhong, Zhenchao Wang, Danping Chen","doi":"10.1021/acs.analchem.4c06200","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c06200","url":null,"abstract":"5-Methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) are crucial epigenetic modifications in eukaryotic genomic DNA that regulate gene expression and are associated with the occurrence of various cancers. Here, we combined bisulfite conversion with 4-acetamido-2,2,6,6-tetramethyl-1-oxopiperridinium tetrafluoroborate (ACT⁺BF₄^–, TCI) oxidation to develop a label-free and sequence-independent isothermal amplification (BTIA) assay for a genome-wide 5mC and 5hmC analysis. The BTIA strategy can distinguish 5mC and 5hmC signatures from other bases with high sensitivity and good specificity, avoiding sophisticated chemical modifications and expensive protein labeling. Moreover, the utilization of terminal deoxynucleotidyl transferase (TdT) enables the proposed strategy to detect global 5mC and 5hmC without sequence dependence. With only 78 ng of input of genomic DNA, global 5mC and 5hmC levels were accurately quantified in cells (including cancer cells of A549, T47D, and K562 and normal cells of HEK-293T, CHO, and NRK-52E) and clinical whole blood samples (including healthy control, precancerous cervical cancer, and confirmed cervical cancer) within 18 h. The detection results suggested that 5mC was highly expressed in cancer cells. More importantly, a significant increase in 5mC was observed in precancerous cervical cancer and further upregulation in confirmed cervical cancer, suggesting a correlation between 5mC and cancer occurrence and development. However, 5hmC showed the reverse result in these tested cells and clinical samples. Collectively, the BTIA strategy can be easily performed on the ordinary heating apparatus in almost all research and medical laboratories, showing a significant application in the early screening of cervical cancer in the clinic.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"59 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoencapsulated Optical Fiber-Based PEC Microelectrode: Highly Sensitive and Specific Detection of NT-proBNP and Its Implantable Performance","authors":"HuiYu Zou, Zhizhong Jiang, ErKang Bian, Jingjing Zhou, Shengqing Li, Yicheng Yang, HuiPing Guo, Yuancheng Liu, WuMing Wu, ChunYan Deng","doi":"10.1021/acs.analchem.4c04757","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c04757","url":null,"abstract":"Microelectrodes offer exceptional sensitivity, rapid response, and versatility, making them ideal for real-time detection and monitoring applications. Photoelectrochemical (PEC) sensors have shown great value in many fields due to their high sensitivity, fast response, and ease of operation. Nevertheless, conventional PEC sensing relies on cumbersome external light sources and bulky electrodes, hindering its miniaturization and implantation, thereby limiting its application in real-time disease monitoring. To overcome these limitations, we developed a nanoencapsulated optical fiber (OF)-based PEC microelectrode. The microelectrode features TiO₂/CdS nanocrystals and bis (2,2′-bipyridine) (10-methylphenanthroline [3,2-<i>a</i>:2′3′-<i>c</i>] pyridine ruthenium(II) dichloride ([Ru(bpy)₂dppz]²⁺) @dsDNA/Au@epigallocatechin gallate nanoparticle (EGCG NP) layers. And its application for the detection of N-terminal pro-brain natriuretic peptide (NT-proBNP) as a biomarker of cardiovascular diseases was explored. An extensive linear range of 1–5000 pg mL^–1 combined with a low detection limit of 0.36 pg mL^–1 was achieved. This range covers not only the recommended threshold for excluding cardiovascular diseases in the clinical diagnosis of individuals across all age groups but also the prognostic target value. The sensor exhibited excellent selectivity and stability and notable labeling recovery capability in serum tests. Critically, the sensor successfully discriminated the alterations in NT-proBNP secretion levels within human smooth muscle cells, comparing pre- and poststimulation by platelet-derived growth factor-BB. Even more significantly, the skin puncture experiment conducted in mice demonstrated the remarkable implantability and biological compatibility of the OF-PEC microelectrode. This addresses critical challenges commonly faced by microelectrodes when used as implanted devices, such as minimizing invasive trauma, mitigating inflammation, and preventing biofouling, thereby firmly establishing their suitability for the development of advanced implantable sensing devices. Therefore, the present OF microelectrode PEC biosensor is not only cost-effective, easy to operate, and miniaturized but also holds significant potential for enabling more precise, more minimally invasive, and continuous monitoring of biological markers without causing inflammation. This capability is crucial for early disease detection, tracking disease progression, and facilitating personalized treatment strategies, which expands the practical application of PEC sensors.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"12 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-Dimensional Liquid Chromatography-Isotope Ratio Mass Spectrometry Opens New Possibilities in the Field of Compound-Specific Stable Isotope Analysis","authors":"Sarah P. Rockel, Robert G. H. Marks, Klaus Kerpen, Maik A. Jochmann, Torsten C. Schmidt","doi":"10.1021/acs.analchem.4c05956","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05956","url":null,"abstract":"Compound-specific stable isotope analysis (CSIA) using liquid chromatography-isotope ratio mass spectrometry (LC-IRMS) is a powerful tool for determining the isotopic composition of carbon in analytes from complex mixtures. However, LC-IRMS methods are constrained to fully aqueous eluents. Previous efforts to overcome this limitation were unsuccessful, as the use of organic eluents in LC-IRMS was deemed impossible. In our study, we developed a two-dimensional (2D) LC-IRMS method that, for the first time, enables the use of organic-containing eluents in an LC-IRMS setting. Initial experiments with caffeine were performed using a sample loop modulator with 20% methanol in the mobile phase of the first dimension, while separating the organic fraction from the analyte in the second dimension. Comparing results with one-dimensional (1D) LC-IRMS methods showed high precision with δ¹³C values in 2D measurements (−34.98 ± 0.04 ‰) closely matching 1D results (−34.95 ± 0.12 ‰). In the next step, incorporation of an at-column dilution (ACD) modulator allowed for the successful use of methanol concentrations up to 40% in the first dimension, with the ACD modulator effectively mitigating both peak fronting and carbon background interference, without losing any precision or accuracy of the measurements (δ¹³C_Caffeine = −34.92 ± 0.03 ‰). All developed methods showed a method detection limit lower than 5 mg of carbon L^–1 (mgC L^–1), which is a major improvement compared with previous studies on caffeine analysis with LC-IRMS. This proof-of-concept study on 2D-LC-IRMS opens vast new possibilities for future CSIA research across diverse fields, including environmental science, pharmaceuticals, and food chemistry.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"120 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface-Sensitive Waveguide Imaging for In Situ Analysis of Membrane Protein Binding Kinetics","authors":"Liangju Li, Jingbo Zhang, Caixin Huang, Yacong Li, Zhaoyang Liu, Jiying Xu, Yi Chen, Ying Zhao, Pengfei Zhang","doi":"10.1021/acs.analchem.4c05510","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05510","url":null,"abstract":"Ligand binding to membrane proteins initiates numerous therapeutic processes. Surface plasmon resonance (SPR), a popular method for analyzing molecular interactions, has emerged as a promising tool for in situ determination of membrane protein binding kinetics owing to its label-free detection, high surface sensitivity, and resistance to intracellular interference. However, the excitation of SPR relies on noble metal films, typically gold, which are biologically incompatible and can cause fluorescence quenching. Here we present a surface-sensitive waveguide imaging technique that retains the advantages of SPR and can be easily integrated into standard SPR devices by depositing additional dielectric layers onto SPR sensor chips using conventional vacuum evaporation. This novel sensor provides a silica surface, a well-known biologically compatible surface, for cell attachment and produces a sharper resonance curve and a stronger surface electric field than SPR, leading to improved measurement precision. After incorporating amplitude modulation, this approach increases measurement precision by approximately eight times compared to traditional SPR with the same optical setup. This high-precision method enables in situ single-cell analysis, revealing cell-to-cell heterogeneity that provides fuel for drug resistance. We believe this technique provides a streamlined strategy to enhance SPR for expanding their applications in biochemical research and drug screening.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"35 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep Learning-Assisted Fluorescence Single-Particle Detection of Fumonisin B1 Powered by Entropy-Driven Catalysis and Argonaute","authors":"Xianfeng Lin, Lixin Kang, Jiaqi Feng, Nuo Duan, Zhouping Wang, Shijia Wu","doi":"10.1021/acs.analchem.4c05913","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05913","url":null,"abstract":"Timely and accurate detection of trace mycotoxins in agricultural products and food is significant for ensuring food safety and public health. Herein, a deep learning-assisted and entropy-driven catalysis (EDC)-Argonaute powered fluorescence single-particle aptasensing platform was developed for ultrasensitive detection of fumonisin B₁ (FB₁) using single-stranded DNA modified with biotin and red fluorescence-encoded microspheres as a signal probe and streptavidin-conjugated magnetic beads as separation carriers. The binding of aptamer with FB₁ releases the trigger sequence to mediate EDC cycle to produce numerous 5′-phosphorylated output sequences, which can be used as the guide DNA to activate downstream <i>Thermus thermophilus</i> Argonaute (<i>Tt</i>Ago) for cleaving the signal probe, resulting in increased number of fluorescence microspheres remaining in the final reaction supernatant after magnetic separation. Subsequently, through fast and accurate counting of red bright particles in the captured confocal fluorescence images from the supernatant via a YOLOv9 deep learning model, the sensitive and specific detection of FB₁ could be realized. This approach has a limit of detection (LOD) of 0.89 pg/mL with a linear range from 1 pg/mL to 100 ng/mL, and satisfactory recovery (87.2–113.5%) in real food samples indicates its practicality. The integration of the aptamer and EDC with <i>Tt</i>Ago broadens the target range of Argonaute and enhances sensitivity. Furthermore, incorporating deep learning significantly improves the analytical efficiency of single-particle detection. This work provides a promising analytical strategy in biosensing and promotes the application of fluorescence single-particle detection in food safety monitoring.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"48 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}