Analytical Chemistry最新文献

{"title":"Precise Modulation of the π-Conjugated Bridge of Naphthalimide-Based Probes for High-Performance Fluorescent Sensing of H2O2","authors":"Ruonan Jiang, Zhenzhen Cai, Huazangnaowu Bai, Yali Liu, Baiyi Zu, Xincun Dou","doi":"10.1021/acs.analchem.5c01015","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01015","url":null,"abstract":"The precise modulation of the π-conjugated bridge within a probe is of considerable importance for the development of reaction-based fluorescent probes endowed with specific functionalities. Here, a series of naphthalimide-based fluorescent probes with a fluorescence ratio and attenuated sensing properties for H₂O₂ have been designed by precisely tuning the type of the π-conjugated bridge located between the boric acid recognizing the moiety and the naphthalimide fluorophore. The modulation of the π-conjugated bridge species mainly focused on three structural units, thiophene, benzene, and furan, among which the thiophene helped us to construct the most efficient naphthalimide-based fluorescent probe (<i>E</i>)-(5-(((2-(2-mercaptoethyl)-1,3-dioxo-2,3-dihydro-1<i>H</i>-benzo[<i>de</i>]isoquinolin-6-yl)imino)methyl)thiophen-2-yl)boronic acid (MOHB-IMTP). It demonstrated the most remarkable fluorescence changes before and after the reaction and thus a significantly superior sensing performance for H₂O₂, including fairly good selectivity and a lower limit of detection (LOD, 38.5 nM). In addition, based on cellulose-induced aggregation of the reaction products, the MOHB-IMTP/cellulose probe was further developed and proven to have a faster and more sensitive (4.0 nM) detection toward H₂O₂. We postulate that this π-conjugated bridge modulation and cellulose signal enrichment strategy will actively promote the development of high-performance fluorescent probes and sensing devices.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"144 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144122719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"QCM Sensing the Motion of Magnetic Particles: Principle and Signal Acquisition","authors":"Dongyu Chen, Yumei Wen, Ping Li, Can Zuo, Yao Wang","doi":"10.1021/acs.analchem.5c01081","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01081","url":null,"abstract":"The traditional quartz crystal microbalance (QCM) technology is primarily used for measuring load mass and requires the load to be in a static state, making it difficult to capture particle motion under the action of external force fields. This study to overcome the constraints of traditional QCM technology by proposing the use of QCM to detect particle motion in liquid loads. This work delves into the principle of QCM sensing particle motion in liquid loads and presents sensing signal models. By investigating the motion mechanism of magnetic particles driven by a magnetic field and generating controllable particle motion, the modulation effect of particle motion on QCM vibration is demonstrated. Experimental results show that particle motion influences the surface strain of the QCM through the liquid medium, modulating the thickness-shear vibration of the QCM. Consequently, particle motion signals can be obtained from the QCM output. Compared to traditional QCM methods that detect static loads, sensing particle motion enables higher sensitivity and stability in detecting parameters (including mass) and allows for the simultaneous detection of multiple load parameters. This study aims to overcome the limitations of traditional QCM technology by proposing a novel approach for detecting particle motion, not only enabling the simultaneous detection of multiple characteristics of the load but also significantly improving detection performance.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"45 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144130157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SONIC: A Speed of Sound Measurement for Nanobubble Characterization","authors":"Jeas Grejoy Andrews, Sunaina, Tatek Temesgen, Peter Kusalik, Kelly Rees, Yihao Wang, W. Russ Algar, Susana Y. Kimura","doi":"10.1021/acs.analchem.5c00781","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00781","url":null,"abstract":"Nanobubbles (NBs)─gas inclusions in water with diameters &lt;1 μm─are of growing interest because of their unique properties and their potential for transformative applications. For example, it has been reported that NBs exist in water over long periods (i.e., weeks to months) and can act as free gas reservoirs. However, NBs are a source of scientific debate, particularly regarding characterization methods. Conventional methods, such as dynamic light scattering, nanoparticle tracking analysis, and nanoflow cytometry, cannot distinguish between nanoparticles and NBs since they are insensitive to the differences of the physical properties of the materials. However, acoustic (speed of sound) measurements can be used to quantify NBs because they rely on the compressibility dependence of gases (κ_gas) which is considerably larger than liquids (κ_water) and solids. In the present work, a speed of sound measurement for nanobubble characterization (SONIC) was designed and developed to probe the compressibility variations diagnostic to NBs in water. NBs in water act as acoustic scatters that reduce the speed of sound relative to the bubble-free water. This decrease in the speed of sound can only be attributed to the existence of gas bubbles due to the strong compressibility dependence that solid nanoparticles lack. The results obtained from the acoustic measurements are compared with the observations from nanoparticle tracking analysis to confirm the existence of NBs in water. SONIC was validated in water with different molalities of NaCl (aq), and in the presence of solid nanoparticles of similar size and concentration to the NBs. SONIC is the first technique that addresses an important bottleneck of NB characterization by providing accurate and selective characterization of NBs in complex water mixtures that will help the behavior of NBs to be better understood and accelerate their application in many fields.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"21 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144130210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fate of Stressed Oligoclonal Antibodies Tracked by Fluorescence Cross-Correlation Spectroscopy: IgG Aggregation Proceeds across the Species Barrier","authors":"Shinya Honda, Akira Sasaki, Yukako Senga, Ayako Ooishi","doi":"10.1021/acs.analchem.5c00396","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00396","url":null,"abstract":"Antibody aggregation is a significant challenge in biopharmaceutical development, affecting drug efficacy and safety. This study applies fluorescence cross-correlation spectroscopy (FCCS) to analyze antibody aggregation, offering a novel approach to understanding this critical process. Our results demonstrated that aggregation can occur without species barriers, as evidenced by the correlated diffusion of different types of stressed antibodies. The absence of a species barrier is significant in elucidating aggregation mechanisms and ensuring the quality of therapeutic antibodies, particularly in oligoclonal formulations. Furthermore, we observed that the aggregation propensity of the antibody fragments (Fab and Fc) differed from that of full-length antibodies, emphasizing the need for a comprehensive analysis of both full-length antibodies and their fragments. Additionally, the self-aggregation propensity of fragments does not always align with their heteroaggregation propensity with preformed antibody aggregates. These findings emphasize the importance of advanced analytical methods to track heterogeneous aggregates accurately and provide insights into the aggregation dynamics of therapeutic antibodies under various conditions.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"56 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144130154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling Ribonucleic Acid Unfolding: A Quantitative Comparison of Solution and Gas-Phase Unfolding.","authors":"Anna G Anders,Jacqueline Anthenien,Ingrid R Kilde,Markos Koutmos,Brandon T Ruotolo","doi":"10.1021/acs.analchem.5c01148","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01148","url":null,"abstract":"Ribonucleic acids (RNAs) are challenging structural biology targets, as numerous barriers exist to determining their high-resolution structures and specific biological functions. Previous results have highlighted the utility of collision-induced unfolding (CIU) to relatively rapidly assess noncoding (nc)RNA higher-order structure (HOS) information. Yet, there remain many gaps in our understanding of how these data can be related to the structures adopted by RNAs in solution as current correlations are largely qualitative. In this study, we describe significant advancements in RNA CIU. Previous RNA CIU reports reveal minimal-to-no RNA unfolding events (or features) upon being subjected to standard CIU conditions. Here, we increase the RNA CIU information through supercharging and quantitatively evaluate the improved RNA CIU data obtained to solution-phase unfolding data collected across a range of Mg2+ concentrations. Finally, we apply our supercharged CIU experiment to mitochondrial encephalopathy, lactic acidosis, and stroke-like episode (MELAS)-associated mt-tRNA leucine (Leu, UUR) (mt-tRNALeu(UUR)) species. Our data demonstrate strong quantitative correlations between gas-phase and solution-phase RNA unfolding events as a function of Mg2+ and MELAS-associated mutations. Taken together, these results indicate strong, solution-relevant relationships for CIU data collected for these RNAs. We conclude our work by discussing future work targeting RNA CIU annotation, broader biophysical characterization of disease-associated RNAs using CIU, and CIU-enabled transcriptomic analysis.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"34 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144122339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-Potential Ratiometric Electrochemiluminescent Immunosensor Modulated by the Single Luminophore Gd-Ru-MOFs","authors":"Beibei Wang,&nbsp;Tong Zhang,&nbsp;Huan Wang,&nbsp;Dawei Fan,&nbsp;Xuejing Liu,&nbsp;Yuyang Li,&nbsp;Dan Wu* and Qin Wei,&nbsp;","doi":"10.1021/acs.analchem.5c0075410.1021/acs.analchem.5c00754","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00754https://doi.org/10.1021/acs.analchem.5c00754","url":null,"abstract":"<p >Multi-signal ratiometric electrochemiluminescence (ECL) sensors have attracted considerable attention owing to their remarkable sensitivity and precision. Here, a novel dual-potential ratiometric ECL sensor has been developed for the ultrasensitive detection of estriol (E3), employing Gd-Ru metal–organic frameworks (Gd-Ru-MOFs) as the single luminophore. The Gd-Ru-MOFs, synthesized with meso-tetra(4-carboxyphenyl)porphine (TCPP) and tris(4,4′-dicarboxylicacid-2,2′-bipyridyl)ruthenium(II) chloride (Ru(H₂dcbpy)₃·Cl₂) as the ligands, are capable of emitting stable cathodic and anodic ECL signals when potassium persulfate (K₂S₂O₈) and triethylamine (TPrA) are present. In order to improve the ECL sensor’s sensitivity, Ce:CuCoO₂, which exhibits a broad ultraviolet absorption spectrum, is utilized as a quencher. This quencher effectively suppresses both cathodic and anodic ECL signals from Gd-Ru-MOFs through resonance energy transfer (RET). Under optimal experimental conditions, the developed ECL sensor demonstrated a linear detection range spanning from 10 fg/mL to 100 ng/mL, with a low detection limit of 3.26 fg/mL (S/N = 3). Finally, the dual-potential ratiometric ECL sensor was successfully applied to the detection of E3 in river. Thus, the proposed strategy has the potential to be extended to other ECL luminophores, offering a novel approach for the development of dual-potential ratiometric ECL sensors.</p>","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"97 21","pages":"11174–11181 11174–11181"},"PeriodicalIF":6.7,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optically Transparent Carbon–Silicon Nitride Windows for Correlative Structural and Electrochemical Analysis of Nanomaterials","authors":"Sasha E. Alden, Oluwasegun J. Wahab, Lingjie Zhang, Kelly L. Vernon, Baixu Zhu, Kathleen O. Bailey, Xingchen Ye, Lane A. Baker","doi":"10.1021/acs.analchem.5c01403","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01403","url":null,"abstract":"We report an optically transparent carbon electrode–silicon nitride (OTCE–SiN_<i>x</i>) window platform fabricated through clean-room microfabrication techniques. A wafer-scale fabrication process was implemented, which enabled a batch preparation of 2925 windows (100 × 100 μm) in user-friendly configurations. Application of OTCE–SiN_<i>x</i> windows is demonstrated in the high-resolution structural and nanoscale electrochemical characterization of nanocrystals by transmission electron microscopy (TEM) and electrochemical scanning probe microscopy. High-resolution TEM (HR-TEM) and aberration-corrected high-angle annular dark-field TEM (HAADF-TEM) were achieved for nanocrystals supported on the OTCE–SiN_<i>x</i>, with minimal background electron scattering or interference from the OTCE–SiN_<i>x</i>. Additionally, the stability of OTCE–SiN_<i>x</i> under prolonged voltammetric cycling was investigated. Scanning electrochemical cell microscopy (SECCM) revealed uniform nanoscale electrochemistry. Correlative electrochemical and electron microscopy on OTCE–SiN_<i>x</i> was demonstrated for the hydrogen evolution reaction (HER) at clusters of sub-10 nm Au nanospheres.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"21 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144122720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing","authors":"Tingting Zhao,&nbsp;Brian Low,&nbsp;Qiming Shen,&nbsp;Yukai Wang,&nbsp;David Hidalgo Delgado,&nbsp;K. N. Minh Chau,&nbsp;Zhiqiang Pang,&nbsp;Xiaoxiao Li,&nbsp;Jianguo Xia,&nbsp;Xing-Fang Li* and Tao Huan*,&nbsp;","doi":"10.1021/acs.analchem.5c0050310.1021/acs.analchem.5c00503","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00503https://doi.org/10.1021/acs.analchem.5c00503","url":null,"abstract":"<p >Over 70% of organic halogens, representing chlorine- and bromine-containing disinfection byproducts (Cl-/Br-DBPs), remain unidentified after 50 years of research. This work introduces a streamlined and cloud-based exposomics workflow that integrates high-resolution mass spectrometry (HRMS) analysis, multistage machine learning, and cloud computing for efficient analysis and characterization of Cl-/Br-DBPs. In particular, the multistage machine learning structure employs progressively different heavy isotopic peaks at each layer and capture the distinct isotopic characteristics of nonhalogenated compounds and Cl-/Br-compounds at different halogenation levels. This innovative approach enables the recognition of 22 types of Cl-/Br-compounds with up to 6 Br and 8 Cl atoms. To address the data imbalance among different classes, particularly the limited number of heavily chlorinated and brominated compounds, data perturbation is performed to generate hypothetical/synthetic molecular formulas containing multiple Cl and Br atoms, facilitating data augmentation. To further benefit the environmental chemistry community with limited computational experience and hardware access, above innovations are incorporated into HalogenFinder (http://www.halogenfinder.com/), a user-friendly, web-based platform for Cl-/Br-compound characterization, with statistical analysis support via MetaboAnalyst. In the benchmarking, HalogenFinder outperformed two established tools, achieving a higher recognition rate for 277 authentic Cl-/Br-compounds and uniquely identifying the number of Cl/Br atoms. In laboratory tests of DBP mixtures, it identified 72 Cl-/Br-DBPs with proposed structures, of which eight were confirmed with chemical standards. A retrospective analysis of 2022 finished water HRMS data revealed insightful temporal trends in Cl-DBP features. These results demonstrate HalogenFinder’s effectiveness in advancing Cl-/Br-compound identification for environmental science and exposomics.</p>","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"97 21","pages":"11099–11109 11099–11109"},"PeriodicalIF":6.7,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lanthanide-Encoded Multi-functional Tetrahedral DNA for Precise Nanodevice Encoding","authors":"Ziyan Li,&nbsp;Jing Zhou,&nbsp;Yiyan Zhu,&nbsp;Rui Liu*,&nbsp;Jianyu Hu and Yi Lv*,&nbsp;","doi":"10.1021/acs.analchem.5c0017510.1021/acs.analchem.5c00175","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00175https://doi.org/10.1021/acs.analchem.5c00175","url":null,"abstract":"<p >The encoding of precise nanodevices is undoubtedly an extremely optimal approach for information storage and multiplex detection. Undeniably, precise control over the nanostructure, composition, and morphology of these devices is of paramount importance. However, most of the tags currently used for encoding are limited by insufficient quantity and low resolution, resulting in deficiencies in accuracy, scalability, and exclusivity of the encoded structures. Here, a series of lanthanide-encoded tetrahedral DNA nanodevices are crafted as unique elemental mass spectrometry-encoded tags. These devices combine the multicomponent interference-free detection capability of elemental encoding with the spatially addressable features of DNA nanostructures. After embedding one to four distinct lanthanide tags (LnTs) and arranging them in equal stoichiometric ratios on different DNA tetrahedral frame cantilevers, the lanthanide nanotags transform into dynamic nanoprobes through combination and fine-tuning. The device can function as a 15-component element tag and generate seven signal outputs. It can respond to three different stimuli when targeting multiple objects simultaneously and is then fed into a semiquantitative analysis based on the isotope dilution method. These DNA nanodevices show strong potential for integration with biological circuits, enabling programmable signal release from their three-dimensional architecture, thereby facilitating even more sophisticated biological identification and logical output.</p>","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"97 21","pages":"11055–11065 11055–11065"},"PeriodicalIF":6.7,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-Amplification CRISPR Biosensor for Ultrasensitive miRNA Detection: A Powerful Tool for Cancer and Immune Monitoring.","authors":"Qirui Liu,Cong Han,Xinying Chang,Zhiqing Zhang,Chao Jia,Liyun Zhang","doi":"10.1021/acs.analchem.5c00732","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00732","url":null,"abstract":"MicroRNAs (miRNAs) are pivotal regulators of gene expression and emerging biomarkers for cancer diagnosis and inflammation monitoring. Sensitive and specific detection methods for miRNAs are crucial for advancing their clinical and research applications. In this study, we present a CRISPR-based biosensor driven by a dual-circle amplification cascade. This system combines rolling circle amplification (RCA) for initial signal amplification and a secondary amplification cycle within the RCA products, enabling precise miRNA expression detection across different cell types. The method is highly sensitive and specific and requires minimal sample input. Using miRNA-221 as a model, the system demonstrated a linear detection range from 15 fM to 1 nM and a detection limit of 1.35 fM. It also effectively differentiated miRNA expression profiles across various cell types, supporting cell classification based on miRNA signatures. Furthermore, the platform's versatility was confirmed with miRNA-155, highlighting its ability to monitor miRNA expression changes in macrophages upon immune stimulation. The system's modular design allows easy adaptation to other miRNA targets, making it a robust tool for miRNA research. This biosensor provides a powerful approach for miRNA profiling in complex biological samples, offering valuable insights into cancer and inflammation with potential for clinical diagnostics and therapeutic monitoring.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"10 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144122039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}