Analytical Chemistry最新文献_第8页

Temperature-Enhanced Purine Metabolism-Based Versatile SERS Platform for Rapid Clinical Pathogens Diagnosis and Drug-Resistant Assessment

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-01-29 DOI: 10.1021/acs.analchem.4c04891

Lei Jin, Qiuqiu Mu, Qing Zhang, Kunxin Li, Ying Wang, Zelong Jiang, Yang Yan, Deyin He, Liqin Zhu, Mengyun Li, Xiangyun Gao, Qi Hui, Jinmei Yang, Xiaojie Wang

{"title":"Temperature-Enhanced Purine Metabolism-Based Versatile SERS Platform for Rapid Clinical Pathogens Diagnosis and Drug-Resistant Assessment","authors":"Lei Jin, Qiuqiu Mu, Qing Zhang, Kunxin Li, Ying Wang, Zelong Jiang, Yang Yan, Deyin He, Liqin Zhu, Mengyun Li, Xiangyun Gao, Qi Hui, Jinmei Yang, Xiaojie Wang","doi":"10.1021/acs.analchem.4c04891","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c04891","url":null,"abstract":"Label-free surface-enhanced Raman spectroscopy (SERS) combined with machine learning (ML) techniques presents a promising approach for rapid pathogen identification. Previous studies have demonstrated that purine degradation metabolites are the primary contributors to SERS spectra; however, generating these distinguishable spectra typically requires a long incubation time (>10 h) at room temperature. Moreover, the lack of attention to spectral variations between strains of the same bacterial species has limited the generalizability of ML models in real-world applications. To address these issues, we investigated temperature-induced alterations in bacterial purine metabolism and found that robust SERS spectra could be obtained within just 1 h by heating samples to 60 °C. Our study further revealed that pathogens exhibit multiple fingerprint patterns across strains, rather than a uniform spectral signature. To enhance practicality, we optimized ML models by training them on data sets capturing all relevant SERS fingerprints and validated them on separate bacterial strains. The SoftMax classifier achieved 100% accuracy in identifying both laboratory and clinical specimens within 17 h. Additionally, the platform demonstrated over 91% accuracy in distinguishing drug-resistant strains, such as methicillin-resistant Staphylococcus aureus and carbapenem-resistant Klebsiella pneumoniae, and achieved 99.66% accuracy in differentiating specific strains within a species, such as enterohemorrhagic Escherichia coli. This accelerated, purine metabolism-based SERS platform offers a highly promising alternative for the rapid diagnosis of bacterial infections.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"59 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143055564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photothermal Desorption and Reagent-Assisted Low-Temperature Plasma Ionization Miniature IT-MS/MS for On-Site Analysis of Illicit Drugs in Saliva and Urine

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-01-28 DOI: 10.1021/acs.analchem.4c05146

Yun Wu, Mei Li, Ruidong Liu, Jing Li, Yingzhe Guo, Dong Yang, Wei Xu, Keyong Hou

{"title":"Photothermal Desorption and Reagent-Assisted Low-Temperature Plasma Ionization Miniature IT-MS/MS for On-Site Analysis of Illicit Drugs in Saliva and Urine","authors":"Yun Wu, Mei Li, Ruidong Liu, Jing Li, Yingzhe Guo, Dong Yang, Wei Xu, Keyong Hou","doi":"10.1021/acs.analchem.4c05146","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05146","url":null,"abstract":"Globally, drug-impaired driving fatalities now exceed those from drunk driving, urging the need for on-site and roadside detection methods. In this study, a photothermal desorption and reagent-assisted low-temperature plasma ionization miniature ion trap mass spectrometer (PDRA-LTP-ITMS) was developed for on-site detection of drug-impaired driving. The pseudomultiple reaction monitoring (MRM) in PDRA-LTP-ITMS enables continuous ion selection during ion introduction and improved sensitivity to nearly 3-fold compared with the conventional full scan mode. The PDRA-LTP integrated the ionization source and photothermal desorption region into the LTP tube with a volume of 0.05 mL. Photoionization and Penning ionization from LTP discharging facilitate proton transfer reactions with the dopant and produce characteristic [M + H]+ for drugs. Dopants of butanol and acetone were separately employed to enhance the thermal desorption and ionization efficiency, resulting in a 2.6-fold sensitivity increase. Saliva and urine samples were collected with a medical swab, and only 10 μL of sample is required for each analysis. The sample is rapidly heated to 250 °C using a halogen lamp and analyzed within 5 s. With these designs, a 4-fold and 10-fold increase in sensitivity was achieved compared to APPI and nano-ESI, respectively. The limits of detection (S/N = 3) of illicit drugs, including MDMA, MDA, methamphetamine, amphetamine, ketamine, and cocaine, in saliva ranged from 4.5 pg μL –1 to 20 pg μL–1 and met the threshold values of GA1333–2017. The performance of the PDRA-LTP-ITMS was even comparable to that of LTQ-Orbitrap Velos Pro ETD MS, providing a novel method of rapid on-site drug-impaired driving analysis.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"36 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143055573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of Resonant Acoustic Fields as Atmospheric-Pressure Ion Gates

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-01-28 DOI: 10.1021/acs.analchem.4c05493

Julia L. Danischewski, Yi You, Lauren Bauer, Jens Riedel, Jacob T. Shelley

{"title":"Use of Resonant Acoustic Fields as Atmospheric-Pressure Ion Gates","authors":"Julia L. Danischewski, Yi You, Lauren Bauer, Jens Riedel, Jacob T. Shelley","doi":"10.1021/acs.analchem.4c05493","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05493","url":null,"abstract":"Ion optics are crucial for spectrometric methods such as mass spectrometry (MS) and ion mobility spectrometry (IMS). Among the wide selection of ion optics, temporal ion gates are of particular importance for time-of-flight MS (TOF-MS) and drift-tube IMS. Commonly implemented as electrostatic ion gates, these optics offer a rapid, efficient means to block ion beams and form discrete ion packets for subsequent analysis. Unfortunately, these devices rely on pulsed high voltage sources and are not fully transparent, even in their open state, which can lead to ion losses and contamination. Here, a novel atmospheric-pressure ion gate based on a resonant acoustic field structure is described. This effect was accomplished through the formation of a resonant, standing acoustic wave of alternating nodes and antinodes. Alignment of an atmospheric-pressure gaseous ion beam with an antinode, i.e. a region of transient pressure, of the acoustic structure acted as a gate and blocked ions from impinging on ion-selective detectors, such as a mass spectrometer and a Faraday plate. The velocity of the ion stream and acoustic power were found to be critical parameters for gating efficiency. In the presence of an acoustic field (i.e., a closed gate), ion signals decreased by as much as 99.8% with a response time faster than the readout of the ion-measurement devices used here (ca. 75 ms). This work demonstrates the basis for a low-cost, acoustic ion gate, which is optically transparent and easily constructed with low-power, off-the-shelf components, that could potentially be used with MS and IMS instrumentation.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"36 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143055577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasmonic Slippery Surface for Surface-Enhanced Raman Spectroscopy and Protein Adsorption Inhibition 用于表面增强拉曼光谱和蛋白质吸附抑制的等离子滑动表面

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-01-28 DOI: 10.1021/acs.analchem.4c01844

Swithin Hanosh, Monisha K, Sajan D. George

{"title":"Plasmonic Slippery Surface for Surface-Enhanced Raman Spectroscopy and Protein Adsorption Inhibition","authors":"Swithin Hanosh, Monisha K, Sajan D. George","doi":"10.1021/acs.analchem.4c01844","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c01844","url":null,"abstract":"Slippery liquid-infused porous surfaces (SLIPSs) are a class of surface that offers low contact angle hysteresis and low tilt angle for water droplet shedding. This property also endows the surface with pinning-free evaporation, which in turn has been exploited for analyte concentration enrichment for Surface Enhanced Raman Spectroscopic applications and antibiofouling. Herein, we demonstrate a facile approach for creating SLIPS with low contact angle hysteresis and low tilt angle for water shedding by coating the equal-volume mixture of polydimethylsiloxane (PDMS) and silicone oil. By exploiting the in situ plasmonic particle reduction capability of the PDMS, the surface is converted to plasmonic SLIPS, which illustrates its potential as a sensitive analytical platform via surface-enhanced Raman spectroscopy. The Raman spectroscopic studies using crystal violet as a reference sample show a limit of detection of 76 pM. Further, we have demonstrated that the fabricated plasmonic substrate is found to be more efficient in inhibiting proteins (bovine serum albumin) on the surface compared to pristine PDMS surfaces. Our fabricated plasmonic surface can find applications in ultrasensitive molecular detection for applications related to analytical chemistry, diagnostics, environmental monitoring, and national security and more importantly can control the nonspecific adsorption of proteins.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"26 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Room-Temperature Selective Detection of H2 by Pd Nanoparticle-Decorated SnO2@WO3 Core–Shell Hollow Structures 钯纳米粒子装饰的 SnO2@WO3 核壳中空结构对 H2 的室温选择性检测

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-01-28 DOI: 10.1021/acs.analchem.4c05215

Yang Bai, Xiaoli Ran, Xiaohong Yang, Shixian Xiong, Feng Gu, Shufen Wang, Jiangcheng Li, Haitao Fu, Xizhong An

{"title":"Room-Temperature Selective Detection of H2 by Pd Nanoparticle-Decorated SnO2@WO3 Core–Shell Hollow Structures","authors":"Yang Bai, Xiaoli Ran, Xiaohong Yang, Shixian Xiong, Feng Gu, Shufen Wang, Jiangcheng Li, Haitao Fu, Xizhong An","doi":"10.1021/acs.analchem.4c05215","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05215","url":null,"abstract":"Sensitive H2 sensors play key roles in the large-scale and safe applications of H2. In this study, we developed novel ternary Pd-loaded SnO2@WO3 core–shell structures by hydrothermal and in situ reduction methods. The compositions of the optimized ternary core–shell structures (Pd-SW-2) are prepared on the basis of the optimal binary core–shell structures (SW-X) according to the sensing performances to H2. Gas sensing tests reveal that the sensing performances (e.g., sensing response and response/recovery time) to H2 are gradually improved after the formation of core–shell structures and the modification of Pd nanoparticles. 10Pd-SW-2 exhibits the highest response (370 times and 204 times higher than those of SnO2 and SW-2, respectively) and the shortest response and recovery time (19/53 s) to 100 ppm of H2 at 25 °C among the as-prepared ternary and binary composites. Combined with the morphology, XPS, electrochemical, H2-TPR, and O2-TPD analyses, the underlying reasons for the improved sensing performance of 10Pd-SW-2 are attributed to (1) the unique core–shell hollow structure and appropriate Pd particle sizes and distribution, (2) abundant oxygen vacancies, (3) the electron sensitization resulting from the energy band structure, and (4) the excellent chemical sensitization originated from the interaction between Pd/PdHx/PdO and H2.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"28 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thin Layer Chromatography Goes Ultrasmall to Assay Sphingosine Kinase Activation in Single Primary Leukemic Cells

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-01-28 DOI: 10.1021/acs.analchem.4c05953

Ming Yao, Yuli Wang, Lucas Cornwell, Christopher E. Sims, Nancy L. Allbritton

{"title":"Thin Layer Chromatography Goes Ultrasmall to Assay Sphingosine Kinase Activation in Single Primary Leukemic Cells","authors":"Ming Yao, Yuli Wang, Lucas Cornwell, Christopher E. Sims, Nancy L. Allbritton","doi":"10.1021/acs.analchem.4c05953","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05953","url":null,"abstract":"Cell-to-cell heterogeneity in lipid signaling underlies variations in response and recurrence for many cancers, including leukemias. A highly parallel, miniaturized thin-layer chromatographic platform capable of assaying single cells was developed. Ultrasmall volumes (50 pL) of standard fluorescent lipids were separated with excellent repeatability, reproducibility, and limits of detection. Sphingosine-cyanine 5 (Sph-Cy5) was loaded into cells, and the single-cell contents were separated to identify Sph-Cy5 and two metabolites, Sph-1-phosphate-Cy5 (S1P-Cy5) and hexadecanoic acid Cy5 (HA-Cy5). In leukemic cells, the CD34+ blast cells demonstrated significantly greater conversion of Sph-Cy5 to its phosphorylated form compared to that of the CD34- cells. After treatment with a sphingosine kinase (SphK) inhibitor, the level of formation of S1P-Cy5 remained significantly greater for the inhibited CD34+ cells relative to that of the inhibited CD34- cells. Over 1200 single cells were rapidly assayed using 8 chips within 4 h. Sphingosine kinase activity in the CD34+ blast cells of 3 patients with acute myeloid leukemia was assayed with and without inhibitors. The patient cells displayed intertumor and intratumor heterogeneity, and subsets of cells with distinct enzymatic activities and products, highlighting the diversity of the cells within a clinical sample and between patients.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"59 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143055574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycan-Matchmade Multivalent Decoration of Enzyme Labels for Amplified Electrochemical Detection of Glycoproteins

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-01-28 DOI: 10.1021/acs.analchem.4c06576

Junpeng Lv, Haibiao Lin, Xiaoxia Chen, Qiong Hu, Li Niu

{"title":"Glycan-Matchmade Multivalent Decoration of Enzyme Labels for Amplified Electrochemical Detection of Glycoproteins","authors":"Junpeng Lv, Haibiao Lin, Xiaoxia Chen, Qiong Hu, Li Niu","doi":"10.1021/acs.analchem.4c06576","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c06576","url":null,"abstract":"Glycoproteins are of significant value to liquid biopsy of human diseases. Herein, we present a universal electrochemical platform for the amplified detection of glycoproteins, taking advantage of the glycan-matchmade multivalent decoration of enzyme labels for the enzymatic signal amplification. Briefly, the glycan-matchmade multivalent decoration involves two steps, i.e., the site-directed decoration of the phenylboronic acid-coated gold nanoparticles (PBA-AuNPs) to the cis-diol-containing glycans of glycoproteins and the subsequent decoration of enzyme labels via the affinity cross-linking between the glycan moieties of enzyme labels and the remaining PBA groups on the PBA-AuNP cross-linkers. As the glycan matchmaking-based strategy enables the decoration of each glycoprotein with multiple enzyme labels, this electrochemical platform exhibits a high sensitivity toward glycoprotein detection. Using alkaline phosphatase (ALP) as the proof-of-concept enzyme label in combination with the solid-state voltammetric stripping assay of the enzymatically deposited metallic silver, the detection limits at the pg mL–1 level have been obtained for the electrochemical aptamer-based detection of thrombin and prostate-specific antigen. Overall, this work illustrates an efficient and versatile strategy for the multivalent decoration of enzyme labels for electrochemical detection of glycoproteins at ultralow concentration levels, holding the desirable advantages of simplicity and cost-effectiveness over sandwich enzyme-linked immunosorbent assays.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"22 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validating Centralized Biobanking Workflows for NMR Metabolomics Using the PRIMA Panel

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-01-28 DOI: 10.1021/acs.analchem.4c04938

Heidi Altmann, Marko Barovic, Katrin Straßburger, Maximilian Tschäpel, Sophie Jonas, David M. Poitz, Alexia Belavgeni, Triantafyllos Chavakis, Peter Mirtschink, Alexander M. Funk

{"title":"Validating Centralized Biobanking Workflows for NMR Metabolomics Using the PRIMA Panel","authors":"Heidi Altmann, Marko Barovic, Katrin Straßburger, Maximilian Tschäpel, Sophie Jonas, David M. Poitz, Alexia Belavgeni, Triantafyllos Chavakis, Peter Mirtschink, Alexander M. Funk","doi":"10.1021/acs.analchem.4c04938","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c04938","url":null,"abstract":"The quality of biological samples used in metabolomics research is significantly influenced by preanalytical factors, such as the timing of centrifugation and freezing. This study aimed to evaluate how preanalytical factors, like delays in centrifugation and freezing, affect metabolomics research. Blood samples, collected in various tube types, were subjected to controlled pre- and postcentrifugation delays. Metabolite levels were quantified using NMR spectroscopy and fitted in linear mixed models used to predict changes in metabolite concentrations over time. The results showed that some metabolites, such as lactic acid, were significantly affected by even short delays, while others remained stable for longer. The study introduced the concept of a “stability time point”, marking when a metabolite’s concentration changes by 20%. These predictive models were validated in a separate cohort. To apply these findings, the authors developed the PRIMA Panel, an open-source R Shiny tool. This tool allows researchers to assess the impact of preanalytical variations on their samples, predict metabolite stability, and generate performance reports. The PRIMA Panel was tested using samples from the Dresden Integrated Liquid Biobank, proving its utility in a real-world biobank setting. The study emphasizes the importance of tracking preanalytical factors to improve the reliability of metabolomics analyses. The PRIMA Panel is available online and for local deployment, providing a practical solution for quality control in metabolomics research. The results of the study underscore the importance of tracking preanalytical factors in biobanking. A versatile tool for assessing their impact on metabolic data is introduced, improving the reliability of future analyses.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"10 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amplifying Electron-Donor Signal of the “Water Gate” Enabled Low Detection Ability of a Field-Effect Transistor-Based Humidity Sensor 放大 "水门 "的电子供体信号使场效应晶体管式湿度传感器的探测能力降低

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-01-28 DOI: 10.1021/acs.analchem.4c04975

Bohao Liu, Qingqing Chen, Jinyong Hu, Yong Zhang

{"title":"Amplifying Electron-Donor Signal of the “Water Gate” Enabled Low Detection Ability of a Field-Effect Transistor-Based Humidity Sensor","authors":"Bohao Liu, Qingqing Chen, Jinyong Hu, Yong Zhang","doi":"10.1021/acs.analchem.4c04975","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c04975","url":null,"abstract":"Low humidity detection down to the parts per million level is urgently demanded in various industrial applications. The hardly detected tiny electrical signal variations caused by a very small amount of water adsorption are one of the intrinsic reasons that restrain the detection limit of the humidity sensors. Herein, a carbon-based field-effect transistor (FET) humidity sensor utilizing adsorbed water as the dual function of a sensing gate and analyte was proposed. Owing to the electron donor property of the “water gate” that can serve as a negative voltage exerted on the dielectric layer, the electrical conductivity of the FET’s channel can be significantly modulated, therefore realizing signal amplification. The proposed sensor presents a detection limit of lower than 1% RH. Besides, the fabricated sensors show good batch consistency (response deviation of 0.5%), repeatability, long-term stability, and acceptable hysteresis (6.3% relative humidity (RH)) in humidity detection. We hope that our work can offer a novel strategy for the application and integration of low humidity detection from the device aspect rather than sensing materials synthesis.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"101 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spontaneously Photocatalytic Nanoplatform for Sensitive Diagnosis and Penetrated Therapy of Cancer

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-01-28 DOI: 10.1021/acs.analchem.4c05185

Huairong Zhang, Lu Feng, Yuqi Wang, Zhiru Bi, Zizhen Wei, Yongbiao Sun, Minghui Shi, Tingting Chen, Linjing Wang, Shusheng Zhang

{"title":"Spontaneously Photocatalytic Nanoplatform for Sensitive Diagnosis and Penetrated Therapy of Cancer","authors":"Huairong Zhang, Lu Feng, Yuqi Wang, Zhiru Bi, Zizhen Wei, Yongbiao Sun, Minghui Shi, Tingting Chen, Linjing Wang, Shusheng Zhang","doi":"10.1021/acs.analchem.4c05185","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05185","url":null,"abstract":"In this study, a sensitive diagnosis and spontaneously photocatalytic therapy of cancer based on chemiluminescence (CL) and nanozyme was studied. Briefly, carbon nitride-supported copper nanoparticles (CuCNs) loaded with luminol (CuCN-L) were utilized to develop a microneedle patch (CuCN-L/MN). The CuCN-L probe could target overexpressed H2O2 in the TME and actively emit CL to achieve cancer cell imaging for diagnosis. In addition, 425 nm blue-violet CL could photocatalyze CuCN to reduce carbon dioxide (CO2) to carbon monoxide (CO) for gas therapy (GT). Meanwhile, CuCN, acting as a photosensitizer (PS), could be activated by CL to produce reactive oxygen species (ROS), which realized photodynamic therapy (PDT). Furthermore, the use of a degradable MN based on hyaluronic acid (HA) promotes painless local administration as well as deep administration of GT and PDT. This technology of optical sensor-mediated anticancer treatment will have great potential to provide new inspirations for cancer diagnosis and therapy based on a self-excited nanoplatform.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"29 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0