Analytical Chemistry最新文献_第7页

Communicating with Stakeholders to Identify High-Impact Research Directions for Non-Targeted Analysis.

IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-01-30 DOI: 10.1021/acs.analchem.4c04801

Sara L Nason, James McCord, Yong-Lai Feng, Jon R Sobus, Christine M Fisher, Ruth Marfil-Vega, Allison L Phillips, Gregory Johnson, John Sloop, Stéphane Bayen, Esra Mutlu, Angela L Batt, Keaton Nahan

{"title":"Communicating with Stakeholders to Identify High-Impact Research Directions for Non-Targeted Analysis.","authors":"Sara L Nason, James McCord, Yong-Lai Feng, Jon R Sobus, Christine M Fisher, Ruth Marfil-Vega, Allison L Phillips, Gregory Johnson, John Sloop, Stéphane Bayen, Esra Mutlu, Angela L Batt, Keaton Nahan","doi":"10.1021/acs.analchem.4c04801","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c04801","url":null,"abstract":"Non-targeted analysis (NTA) using high-resolution mass spectrometry without defined chemical targets has the potential to expand and improve chemical monitoring in many fields. Despite rapid advancements within the research community, NTA methods and data remain underutilized by many potential beneficiaries. To better understand barriers toward widespread adoption, the Best Practices for Non-Targeted Analysis (BP4NTA) working group conducted focus group meetings and follow-up surveys with scientists (n = 61) from various sectors (e.g., drinking water utilities, epidemiologists, n = 9) where NTA is expected to provide future value. Meeting participants included producers and end-users of NTA data with a wide range of familiarity with NTA methods and outputs. Discussions focused on identifying specific barriers that limit adoption and on setting NTA product development priorities. Stated priorities fell into four major categories: 1) education and training materials; 2) QA/QC frameworks and study design guidance; 3) accessible compound databases and libraries; and 4) NTA data linkages with chemical fate and toxicity information. Based on participant feedback, this manuscript proposes research directions, such as standardization of training materials, that BP4NTA and other institutions can pursue to expand NTA use in various application scenarios and decision contexts.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":" ","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

sgRNA Single-Nucleotide Resolution by Ion-Pairing Reversed-Phase Chromatography.

IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-01-30 DOI: 10.1021/acs.analchem.4c05325

Joshua D Jones, Todd D Maloney

{"title":"sgRNA Single-Nucleotide Resolution by Ion-Pairing Reversed-Phase Chromatography.","authors":"Joshua D Jones, Todd D Maloney","doi":"10.1021/acs.analchem.4c05325","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05325","url":null,"abstract":"Single-stranded guide RNAs (sgRNAs) are important therapeutic modalities that facilitate selective genome editing by the CRISPR/Cas9 system. While these therapeutic modalities are synthesized through solid phase oligonucleotide synthesis similar to small interfering RNA (siRNAs) and antisense oligonucleotide (ASOs) therapeutics, their sequence length and complex secondary and tertiary structure hinder analytical characterization. The resulting current sgRNA methodologies have limited chromatographic selectivity near the FLP and limited MS compatibility. Here, we perform a systematic evaluation of chromatographic parameters to develop a highly selective ion-pairing reversed-phase chromatography separation for sgRNAs. We identify that stronger and more hydrophobic ion-pairing reagents promote the selectivity for long truncation impurities. Further, reduced flow rates and temperatures promote selectivity near the FLP. Together, this study reports the first single-nucleotide resolution chromatography method for an sgRNA modality while employing a highly MS-compatible mobile phase. This work provides further evidence that the sgRNA chromatographic selectivity is highly independent of on-column mass transfer. This methodology will enable the high-resolution characterization of sgRNA therapeutics, providing further insights into impurity profiles to facilitate toxicological studies and process development of these genetic medicines.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":" ","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exogenous Coreactant-Free Electrocatalytic Reactive Oxygen Species-Driven Dual-Signal Molecularly Imprinted Electrochemiluminescence Sensor for the Detection of Trenbolone

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-01-30 DOI: 10.1021/acs.analchem.4c06850

Yongqiang Li, Kaida Kuang, Yang Chen, Xuan Chen, Qinghua Cheng, Ya Li, Lijuan Liang, Nengqin Jia

{"title":"Exogenous Coreactant-Free Electrocatalytic Reactive Oxygen Species-Driven Dual-Signal Molecularly Imprinted Electrochemiluminescence Sensor for the Detection of Trenbolone","authors":"Yongqiang Li, Kaida Kuang, Yang Chen, Xuan Chen, Qinghua Cheng, Ya Li, Lijuan Liang, Nengqin Jia","doi":"10.1021/acs.analchem.4c06850","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c06850","url":null,"abstract":"Conventional dual-signal electrochemiluminescence (ECL) sensors feature high sensitivity and reliability, but the involvement of coreactants inevitably results in a complex configuration and shows reproducibility risk. Here, we propose an exogenous coreactant-free dual-signal platform, comprising luminol (anodic luminophore), CdSe quantum dots (cathodic luminophore), and Co3O4/Ti3C2 electrocatalyst (coreaction promoter). At different redox potentials, Co3O4/Ti3C2 induces water oxidation and oxygen reduction to produce •OH and O2•– radicals, which subsequently drive cathodic and anodic ECL emission, respectively. The dual-signal ECL pathways are confirmed by reactive oxygen species (ROS) scavenging, and the effect of dissolved oxygen is excluded. By integrating with molecular imprinting technology, this dual-signal ECL sensor is employed for the quantitative detection of trenbolone. It exhibits high sensitivity, broad linear range (1.0 × 101 to 1.0 × 108 fg mL–1), low detection limit, and detection capability of real samples. This work presents a new endogenous ROS-driven dual-signal ECL sensor that excludes the use of exogenous coreactants and offers new insights into ROS generation in a coreactant-free sensor design.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"57 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Single-Molecule Biosensor Based on Polymerization-Transcription-Mediated Target Regeneration for Simultaneously One-Pot Detection of Multiple piRNAs

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-01-30 DOI: 10.1021/acs.analchem.4c06491

Dong-Ming Yang, Dongling Li, Qian Zhang, Shulin Zhao, Chun-Yang Zhang

{"title":"Development of a Single-Molecule Biosensor Based on Polymerization-Transcription-Mediated Target Regeneration for Simultaneously One-Pot Detection of Multiple piRNAs","authors":"Dong-Ming Yang, Dongling Li, Qian Zhang, Shulin Zhao, Chun-Yang Zhang","doi":"10.1021/acs.analchem.4c06491","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c06491","url":null,"abstract":"PIWI-interacting RNAs (piRNAs) are a class of small noncoding RNAs associated with PIWI proteins within the male germline, and they play significant roles in maintaining genome stability via the modulation of gene expression. The piRNAs are implicated in the progression of various cancers, but the simultaneous monitoring of multiple piRNAs remains a challenge. Herein, we construct a single-molecule biosensor based on polymerization-transcription-mediated target regeneration for the simultaneous one-pot detection of multiple piRNAs. This assay involves two cycles. In step 1, target piRNAs hybridize with the template probe complexes to yield three-way junction (3WJ) structures. Then, KF DNA polymerase initiates the extension to generate a complete T7 promoter, and the extension product can act as an invading strand to displace signal probes, resulting in the release of fluorophores. Then, in step 2, the T7 promoter can be recognized by T7 RNA polymerase to initiate transcription, producing abundant transcripts with 3′–OH that are identical to piRNAs. The resultant transcripts can hybridize with free template probe complexes to obtain new 3WJ structures that can be elongated by KF polymerase for the recovery of fluorescence signals. This assay can be performed homogeneously in a one-pot format within 30 min, and it exhibits high sensitivity, with a limit of detection (LOD) of 19.26 aM for piRNA-36026 and 41.88 aM for piRNA-36743. Moreover, it can simultaneously detect endogenous piRNAs at the single-cell level and differentiate piRNA expression in the tissues of healthy individuals and breast cancer patients, offering a prospective platform for clinic diagnosis.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"17 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiplex Nontargeted Framework Enables Tracking Metabolic Profile of Oxymetholone and Methasterone In Vivo at Nanogram Level by GC-Orbitrap-HRMS for Antidoping Purpose

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-01-30 DOI: 10.1021/acs.analchem.4c06026

Siying Zheng, Yuqi Ge, Xian Fang, Mengpan Liu, Haoyi Sun, Xiaojun Deng, Lei Liao

{"title":"Multiplex Nontargeted Framework Enables Tracking Metabolic Profile of Oxymetholone and Methasterone In Vivo at Nanogram Level by GC-Orbitrap-HRMS for Antidoping Purpose","authors":"Siying Zheng, Yuqi Ge, Xian Fang, Mengpan Liu, Haoyi Sun, Xiaojun Deng, Lei Liao","doi":"10.1021/acs.analchem.4c06026","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c06026","url":null,"abstract":"Oxymetholone and methasterone are anabolic androgenic steroids prohibited by the World Anti-Doping Agency (WADA) for both in-competition and out-of-competition use. Detecting metabolites of exogenous steroids is crucial for establishing doping violations, making the study of these metabolites essential in antidoping efforts. This study investigated the urinary metabolic profiles of oxymetholone and methasterone using gas chromatography-orbitrap high-resolution mass spectrometry (GC-Orbitrap-HRMS) in nanogram level by utilizing a novel multiplex nontargeted framework protocol. Healthy volunteers each ingested one tablet of the drug, and urine samples were collected over 50 days in postadministration phase. The complete detection of the three fractions (free fraction, glucuronide fraction, and sulfate fraction) of the metabolites was carried out. The GC-Orbitrap-HRMS full-scan mode was employed to detect postadministration urine samples, comparing these with preadministration baseline urine samples to identify newly formed substances. Electron ionization (EI) mass spectra were used to infer possible metabolite structures, leading to the discovery of three novel metabolites of oxymetholone and two novel metabolites of methasterone. Notably, the newly identified oxymetholone metabolite, 2-methylene-17α-methyl-androstane-16ξ,17β-diol-3-one (O-M6), was detectable as a glucuronide conjugate up to 4 days after administration. The methasterone metabolite, 18-nor-17β-hydroxymethyl-2α,17α-dimethyl-5α-androst-13-en-3-one (M-M4), exhibited prolonged detectability as a glucuronide conjugate, being present in urine samples from both volunteers up to 50 days after administration. These findings have significant implications for antidoping purpose, providing extended detection windows for these anabolic steroids.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"84 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Oral Fluid Stains on Fabric via Solution Extraction Combined with Deep Ultraviolet Raman Spectroscopy.

IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-01-30 DOI: 10.1021/acs.analchem.4c04581

Alexis Weber, Mohamed O Amin, Vladimir Ermolenkov, Entesar Al-Hetlani, Igor K Lednev

{"title":"Detection of Oral Fluid Stains on Fabric via Solution Extraction Combined with Deep Ultraviolet Raman Spectroscopy.","authors":"Alexis Weber, Mohamed O Amin, Vladimir Ermolenkov, Entesar Al-Hetlani, Igor K Lednev","doi":"10.1021/acs.analchem.4c04581","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c04581","url":null,"abstract":"DNA phenotyping plays a central role in modern practical forensics, yet an overwhelming amount of evidence creates significant backlogs in all major crime laboratories. A fast nondestructive test of a potential biological stain prior to DNA phenotyping should reduce the number of irrelevant samples for the analysis and increase the efficiency of the overall process. Evidence items recovered from the crime scene can often include body fluid traces, such as oral fluid (OF). This proof-of-concept study demonstrates the effectiveness of Deep-UV Raman spectroscopy in identifying OF stains on substrates such as cotton, polyester, and blue denim, commonly encountered in forensic investigations. Through spectral interpretation and statistical analysis, this study compares Raman spectra from OF extracted from substrates with pure OF spectra. Additionally, comparative analysis with near-infrared (NIR) Raman spectroscopy to deep-UV Raman spectroscopy was performed. Distinct advantages of deep-UV Raman spectroscopy were determined, including reduced sample preparation requirements and the absence of fluorescence background, enhancement of the signal-to-noise ratio, and simplified data preprocessing. Using statistical analysis methods like principal component analysis and partial least-squares discriminate analysis, differentiation between OF and non-OF samples was possible. Overall, this study underscores the versatility and potential of deep-UV Raman spectroscopy as a valuable tool in forensic science.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":" ","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aptamer-Modified Ti3C2Tx MXene Fluorescent Nanoprobe for Monitoring ATP and GTP during a Mild-Photothermal-Activated Nucleolar Stress Process in Living Cells

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-01-30 DOI: 10.1021/acs.analchem.4c04959

Xin Guan, Jiaxin Wang, Guohua Qi, Limei Chen, Bo Wang, Yongdong Jin

{"title":"Aptamer-Modified Ti3C2Tx MXene Fluorescent Nanoprobe for Monitoring ATP and GTP during a Mild-Photothermal-Activated Nucleolar Stress Process in Living Cells","authors":"Xin Guan, Jiaxin Wang, Guohua Qi, Limei Chen, Bo Wang, Yongdong Jin","doi":"10.1021/acs.analchem.4c04959","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c04959","url":null,"abstract":"Understanding the molecular energy metabolism of single cells in the nucleolus stress response induced by mild-photothermal therapy (mPTT) is of great importance for investigating the photothermal lethal mechanism. Herein, we successfully fabricated a “turn-on”-type fluorescent nanoprobe based on the fluorescently labeled aptamers (FAM-ATP-apt and Cy3-GTP-apt) and Ti3C2TX MXene. When the adapters on the nanoprobes bonded to intracellular ATP and GTP, the fluorescence of the nanoprobes was restored. We used the nanoprobes to monitor the variation of ATP and GTP contents in individual tumor cells and normal cells during the mPTT. Interestingly, we found that NPM1 translocated from the nucleolus to the nucleoplasm in MCF-7 cancer cells within 12 min of mPTT laser irradiation, which did not occur in normal MCF-10A cells. However, the content changes of ATP and GTP in tumor cells during the mPTT treatment were significantly higher than that of normal cells. The developed Ti3C2Tx-ATP/GTP nanoprobe enables simultaneous imaging of ATP and GTP in single living cells for bioimaging and molecular detection applications.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"17 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid Curvature and Fluidity Influence Lipid Incorporation Disparities in Nanodiscs.

IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-01-30 DOI: 10.1021/acs.analchem.4c05494

Marina C Sarcinella, Joshua D Jones, Matthew J Sorensen, Samantha A Edgcombe, Brandon T Ruotolo, Robert T Kennedy, Ryan C Bailey

{"title":"Lipid Curvature and Fluidity Influence Lipid Incorporation Disparities in Nanodiscs.","authors":"Marina C Sarcinella, Joshua D Jones, Matthew J Sorensen, Samantha A Edgcombe, Brandon T Ruotolo, Robert T Kennedy, Ryan C Bailey","doi":"10.1021/acs.analchem.4c05494","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05494","url":null,"abstract":"Nanodiscs have become a popular membrane mimetic system offering a well-defined bilayer environment to stabilize membrane proteins for in vitro analyses using a range of analytical methods; however, lipid compositions common to their deployment are simplistic and often fail to model native membrane complexity. Furthermore, there has been a general lack of rigorous analytical and biophysical characterization of nanodiscs comprising more than one lipid. To address these challenges, we coupled a nanodisc formation and purification workflow with targeted LC-MS/MS analysis to quantify lipids in nanodiscs made with different compositions. We screened lipids with a variety of headgroups and acyl chains and found that lipids did not always incorporate into nanodiscs at expected levels. Disparities in lipid incorporation were found to increase upon the addition of lipids known to induce curvature or rigidity to the membrane. Additionally, we found that adding just one additional type of lipid to nanodiscs changes the particle diameter and dispersity compared to nanodiscs containing a single lipid. We also formed and characterized nanodiscs using a complex starting composition inspired by the endoplasmic reticulum membrane and observed native-like cholesterol dynamics that modulated the lipid fluidity in the model bilayer system. Taken together, this work serves as a foundation for understanding nonstoichiometric lipid incorporation into nanodiscs and provides a basis for more thorough nanodisc characterization and quality control, which is critical to ensure multilipid nanodiscs synthesized accurately model the biological system of interest, enabling robust characterization of how the lipid landscape affects membrane protein structure and activity.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":" ","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxygen Activation Biocatalytic Precipitation Strategy Based on a Bimetallic Single-Atom Catalyst for Photoelectrochemical Biosensing.

IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-01-30 DOI: 10.1021/acs.analchem.4c06447

Hongkun Li, Zhikang Li, Qianqian Cai, Guifen Jie

{"title":"Oxygen Activation Biocatalytic Precipitation Strategy Based on a Bimetallic Single-Atom Catalyst for Photoelectrochemical Biosensing.","authors":"Hongkun Li, Zhikang Li, Qianqian Cai, Guifen Jie","doi":"10.1021/acs.analchem.4c06447","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c06447","url":null,"abstract":"The traditional biocatalytic precipitation (BCP) strategy often required the participation of H2O2, but H2O2 had the problem of self-decomposition, which prevented its application in quantitative analysis. This work first found that a bimetallic single-atom catalyst (Co/Zn-N-C SAC) could effectively activate dissolved O2 to produce reactive oxygen species (ROS) due to its superior oxidase (OXD)-like activity. Experimental investigations demonstrated that Co/Zn-N-C SAC preferred to produce highly active hydroxyl radicals (•OH), which oxidized 3-amino-9-ethyl carbazole (AEC) to produce reddish-brown insoluble precipitates. Based on this property, a unique oxygen-activated photoelectrochemical (PEC) biosensor was developed for chloramphenicol (CAP) detection. Cesium platinum bromide nanocrystals (Cs2PtBr6 NCs) were a new type of halide perovskite with lead-free, narrow band gaps, and water-oxygen resistance. Cs2PtBr6 NCs showed excellent cathodal PEC performance without an exogenous coreactant and were first used for PEC detection. As a \"proof-of-concept application\", Co/Zn-N-C SAC was introduced onto the surface of Cs2PtBr6 NCs by using the CAP dual-aptamer sandwich strategy. Co/Zn-N-C SAC activated dissolved O2 to produce ROS, which oxidized AEC to produce precipitates, quenching the cathodal PEC signal of Cs2PtBr6 NCs for CAP detection. In summary, this work first used SAC to overcome the restriction of the traditional enzymatic BCP strategy requiring H2O2, improved the stability and accuracy of quantitative analysis, and also broadened the application range of coreactant-free perovskite-type PEC biosensors.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":" ","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic Field-Accelerated Nonthermal Plasma Digestion for Field Pretreatment and Determination of Heavy Metals in Biological Samples

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-01-30 DOI: 10.1021/acs.analchem.4c05556

Ji-Ying Cai, Hao Shen, Jia-Nan Xu, Jia-Min Xu, Jian-Hua Wang, Yong-Liang Yu

{"title":"Magnetic Field-Accelerated Nonthermal Plasma Digestion for Field Pretreatment and Determination of Heavy Metals in Biological Samples","authors":"Ji-Ying Cai, Hao Shen, Jia-Nan Xu, Jia-Min Xu, Jian-Hua Wang, Yong-Liang Yu","doi":"10.1021/acs.analchem.4c05556","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05556","url":null,"abstract":"Field analysis of heavy metals in biological samples is essential for assessing their potential threats to human health. The development of portable pretreatment and detection devices is crucial to address this challenge. Herein, a magnetic field-accelerated nonthermal plasma digestion device using dielectric barrier discharge (DBD) is designed for the rapid and environmentally friendly pretreatment of biological samples and subsequently combined with point discharge-optical emission spectrometry (PD-OES) for sensitive determination of heavy metals. With the assistance of a magnetic field, the DBD plasma digestion of a batch of six samples using a H2O + H2O2 + HNO3 system can be completed within 25–40 min, achieving the digestion efficiencies of 92–99%. The application of magnetic field enhances the electron density, excitation temperature, discharge current, and power of the DBD plasma, thereby reducing the digestion time by 40–69%. The determination of heavy metals in the digestion solution is directly performed using a miniature injection-detector based on hydride generation-PD-OES. Under the optimized conditions, the detection limits for Pb, Sb, Sn, and Bi are 2.4–8.2 μg L–1 (0.048–0.16 mg kg–1), with precisions of <5%. The accuracy and practicability of the present device are verified by measuring several certified reference materials, including GBW10023 (laver) and GBW10044 (rice), and real rice, laver, fish, milk, and blood samples. With its advantages of environmental sustainability, short digestion time, compact size, and lightweight design, the present device provides a simple and efficient tool for field analysis of toxic elements in biological samples.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"27 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0