Journal of Materials Chemistry B最新文献

Tissue adhesives based on chitosan for biomedical applications 基于壳聚糖的组织粘合剂在生物医学中的应用

IF 6.331 3区医学

Journal of Materials Chemistry B Pub Date : 2024-09-18 DOI: 10.1039/d4tb01362j

Jihyun Youn, Kapil D. Patel, Adam W. Perriman, Jung-Suk Sung, Madhumita Patel, Louis-S. Bouchard, Rajkumar Patel

引用次数: 0

Development and evaluation of 3D composite scaffolds with piezoelectricity and biofactor synergy for enhanced articular cartilage regeneration 开发和评估具有压电性和生物因子协同作用的三维复合支架，促进关节软骨再生

IF 6.331 3区医学

Journal of Materials Chemistry B Pub Date : 2024-09-18 DOI: 10.1039/d4tb01319k

Bowen Xie, Hebin Ma, Fengyuan Yang, Hongguang Chen, Ya’nan Guo, Hongxing Zhang, Tengfei Li, Xiaogang Huang, Yantao Zhao, Xiaojie Li, Junjie Du

{"title":"Development and evaluation of 3D composite scaffolds with piezoelectricity and biofactor synergy for enhanced articular cartilage regeneration","authors":"Bowen Xie, Hebin Ma, Fengyuan Yang, Hongguang Chen, Ya’nan Guo, Hongxing Zhang, Tengfei Li, Xiaogang Huang, Yantao Zhao, Xiaojie Li, Junjie Du","doi":"10.1039/d4tb01319k","DOIUrl":"https://doi.org/10.1039/d4tb01319k","url":null,"abstract":"The inability of articular cartilage to self-repair following injuries frequently precipitates osteoarthritis, profoundly affecting patients' quality of life. Given the limitations inherent in current clinical interventions, an urgent need exists for more effective cartilage regeneration methodologies. Previous studies have underscored the potential of electrical stimulation in cartilage repair, thus motivating the investigation of innovative strategies. The present study introduces a three-dimensional scaffold fabricated through a composite technique that leverages the synergy between piezoelectricity and biofactors to enhance cartilage repair. This scaffold is composed of polylactic acid (PLLA) and barium titanate (BT) for piezoelectric stimulation and at the bottom with a collagen-coated layer infused with fibroblast growth factor-18 (FGF-18) for biofactor delivery. Designed to emulate the properties of natural cartilage, the scaffold enables controlled generation of piezoelectric charges and the sustained release of biofactors. In vitro tests confirm that the scaffold promotes chondrocyte proliferation, matrix hyperplasia, cellular migration, and the expression of genes associated with cartilage formation. Moreover, in vivo studies on rabbits have illustrated its efficacy in catalyzing the in situ regeneration of articular cartilage defects and remodeling the extracellular matrix. This innovative approach offers significant potential for enhancing cartilage repair and holds profound implications for regenerative medicine.","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":6.331,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142248576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photopatterning of conductive hydrogels which exhibit tissue-like properties 具有类似组织特性的导电水凝胶的光图案化

IF 6.331 3区医学

Journal of Materials Chemistry B Pub Date : 2024-09-16 DOI: 10.1039/d4tb00807c

Léo Sifringer, Lina De Windt, Stéphane Bernhard, Giulia Amos, Blandine Clément, Jens Duru, Mark W. Tibbitt, Christina M. Tringides

{"title":"Photopatterning of conductive hydrogels which exhibit tissue-like properties","authors":"Léo Sifringer, Lina De Windt, Stéphane Bernhard, Giulia Amos, Blandine Clément, Jens Duru, Mark W. Tibbitt, Christina M. Tringides","doi":"10.1039/d4tb00807c","DOIUrl":"https://doi.org/10.1039/d4tb00807c","url":null,"abstract":"Hydrogels are three-dimensional, highly tunable material systems that can match the properties of extracellular matrices. In addition to being widely used to grow and modulate cell behavior, hydrogels can be made conductive to further modulate electrically active cells, such as neurons, and even incorporated into multielectrode arrays to interface with tissues. To enable conductive hydrogels, graphene flakes can be mechanically suspended into a hydrogel precursor. The conductivity of the hydrogel can be increased by increasing the weight percentage of graphene flakes in the precursor while maintaining the mechanical properties of the formed gel similar to the properties of neural tissue. By using a photocrosslinkable hydrogel matrix, such as gelatin methacrylate, with a photoabsorber, the conductive precursor solutions can be crosslinked into predefined complex patterns. Finally, the formulations can be used to support the growth of sensory neurons, derived from human induced pluripotent stem cells, for more than 7 weeks while the neurons remain viable. These scaffolds can be patterned into components of multielectrode arrays, to enable ultrasoft electrodes with tissue-matched properties for further interactions, both in vitro and in vivo, with the nervous systems.","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":6.331,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142248575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vivo safety evaluation and tracing of arginylglycylaspartic acid-engineered phage nanofiber in murine model 精氨酰甘氨酰天冬氨酸工程噬菌体纳米纤维在小鼠模型中的体内安全性评估和追踪研究

IF 6.331 3区医学

Journal of Materials Chemistry B Pub Date : 2024-09-16 DOI: 10.1039/d4tb00823e

Kshitiz Raj Shrestha, Sehoon Kim, Anna Jo, Murali Ragothaman, So Young Yoo

引用次数: 0

A versatile fluorescent probe for hydrogen peroxide in serotonergic neurons of living brains of mice with depression 抑郁症小鼠活体大脑血清素能神经元中过氧化氢的多功能荧光探针

IF 6.331 3区医学

Journal of Materials Chemistry B Pub Date : 2024-09-16 DOI: 10.1039/d4tb01828a

Feida Che, Xiaoming Zhao, Qi Ding, Xiwei Li, Wen Zhang, Ping Li, Xin Wang, Bo Tang

{"title":"A versatile fluorescent probe for hydrogen peroxide in serotonergic neurons of living brains of mice with depression","authors":"Feida Che, Xiaoming Zhao, Qi Ding, Xiwei Li, Wen Zhang, Ping Li, Xin Wang, Bo Tang","doi":"10.1039/d4tb01828a","DOIUrl":"https://doi.org/10.1039/d4tb01828a","url":null,"abstract":"Depression, a prevalent mental illness, is intricately linked with the neurotransmitters in the brain, while serotonin as a crucial regulator of mood, energy levels, and memory, has been implicated in depression. So, the release of serotonin by serotonergic neurons plays a significant role in the development of depression. Notably, the foremost marker of oxidative stress, hydrogen peroxide (H2O2), can interfere with the functioning of serotonergic neurons and potentially contribute to depression. Investigating the impact of H2O2 on serotonergic neurons could offer valuable insights into the mechanisms underlying depression. However, there have been no effective tools for selectively imaging H2O2 in these neurons so far. To address this gap, we created a small molecular fluorescent probe, PF-H2O2, designed specifically for imaging H2O2 in serotonergic neurons under oxidative stress. PF-H2O2 exerts excellent serotonergic neuron-targetability and notable selectivity for H2O2. Furthermore, we discovered increased H2O2 in serotonergic neurons of mice with depressive symptoms. Altogether, this endeavour unveils a pioneering tool for exploring pathophysiology linked to serotonergic neuronal dysfunction.","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":6.331,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142248623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Encapsulation of telmisartan inside insulinoma-cell-derived extracellular vesicles outperformed biomimetic nanovesicles in modulating the pancreatic inflammatory microenvironment 在胰岛素瘤细胞衍生的细胞外囊泡内包裹替米沙坦在调节胰腺炎症微环境方面的效果优于仿生纳米囊泡

IF 6.331 3区医学

Journal of Materials Chemistry B Pub Date : 2024-09-13 DOI: 10.1039/d4tb00808a

Anjali Singh, Subrata Kumar Pore, Jayanta Bhattacharyya

{"title":"Encapsulation of telmisartan inside insulinoma-cell-derived extracellular vesicles outperformed biomimetic nanovesicles in modulating the pancreatic inflammatory microenvironment","authors":"Anjali Singh, Subrata Kumar Pore, Jayanta Bhattacharyya","doi":"10.1039/d4tb00808a","DOIUrl":"https://doi.org/10.1039/d4tb00808a","url":null,"abstract":"Diabetes mellitus (DM) is a chronic metabolic condition, characterized by hyperglycaemia, oxidative imbalance, pancreatic β-cell death, and insulin insufficiency. Angiotensin II (Ang II) increases oxidative stress, inflammation, and apoptosis, and Ang II type 1 receptor (AT1R) blockers (ARBs) can ameliorate inflammatory response and oxidative stress. However, like other small-molecule drugs, free ARBs show poor in vivo efficacy and dose-limiting toxicities. Hence, in this study, we developed nano-formulations of telmisartan (TEL), an ARB, by encapsulating it inside a murine insulinoma cell-derived extracellular vesicle (nanoTEL) and a bio-mimetic lipid nanovesicle (lipoTEL). Both nano-formulations showed spherical morphology and sustained release of TEL. In vitro, nanoTEL restored oxidative equilibrium, attenuated reactive oxygen species levels, enhanced the uptake of glucose analogue, and increased the expression of glucose transporter protein 4 better than lipoTEL. In a streptozotocin-induced murine model of diabetes, nanoTEL lowered blood glucose levels, improved glucose tolerance, and promoted insulin synthesis and secretion significantly better than lipoTEL. Moreover, nanoTEL was found superior in ameliorating the pancreatic inflammatory microenvironment by regulating NF-κBp65, HIF-1α, and PPAR-γ expression; modulating IL-1β, IL-6, tumor necrosis factor-α, IL-10, and IL-4 levels and inducing the polarization of macrophage from M1 to M2. Further, nanoTEL administration induced angiogenesis and promoted the proliferation of pancreatic cells to restore the structural integrity of the islets of Langerhans more efficiently than lipoTEL. These findings collectively suggest that nanoTEL outperforms lipoTEL in restoring the function of pancreatic β-cells by modulating the pancreatic inflammatory microenvironment and show potential for the treatment of DM.","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":6.331,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142215161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the potential of an Aloe vera and honey extract loaded bi-layered nanofibrous scaffold of PCL-Col and PCL-SBMA mimicking the skin architecture for the treatment of diabetic wounds 探索负载芦荟和蜂蜜提取物的 PCL-Col 和 PCL-SBMA 双层纳米纤维支架模拟皮肤结构治疗糖尿病伤口的潜力

IF 6.331 3区医学

Journal of Materials Chemistry B Pub Date : 2024-09-13 DOI: 10.1039/d4tb01469c

Megha Dhiman, Souvik Ghosh, Thakur Gurjeet Singh, Samrat Chauhan, Partha Roy, Debrupa Lahiri

{"title":"Exploring the potential of an Aloe vera and honey extract loaded bi-layered nanofibrous scaffold of PCL-Col and PCL-SBMA mimicking the skin architecture for the treatment of diabetic wounds","authors":"Megha Dhiman, Souvik Ghosh, Thakur Gurjeet Singh, Samrat Chauhan, Partha Roy, Debrupa Lahiri","doi":"10.1039/d4tb01469c","DOIUrl":"https://doi.org/10.1039/d4tb01469c","url":null,"abstract":"Diabetic wounds are often chronic in nature, and issues like elevated blood sugar, bacterial infections, oxidative stress and persistent inflammation impede the healing process. To ensure the appropriate healing of wounds, scaffolds should promote complete tissue regeneration in wounds, both functionally and structurally. However, the available scaffolds lack the explicit architecture and functionality that could match those of native skin, thus failing to carry out the scar-free skin regeneration in diabetic wounds. This study deals with the synthesis of a bi-layered nanofibrous scaffold mimicking the native skin architecture in terms of porosity and hydrophobic–hydrophilic gradients. In addition, herbal extracts of Aloe vera and litchi honey were added in consecutive layers to manage the high blood glucose level, inflammation, and increased ROS level associated with diabetic wounds. In vitro studies confirmed that the prepared scaffold with herbal extracts showed enhanced proliferation of skin cells with good mechanical strength, degradability, anti-bacterial and anti-diabetic properties. The scaffold also demonstrated superior wound healing in vivo with quicker scar-free wound recovery and appropriate skin regeneration, compared to conventional treatment. Altogether, the synthesized herbal extract loaded bi-layered nanofibrous scaffold can be used as a regenerative template for hard-to-heal diabetic wounds, offering a new strategy for the management of chronic wounds.","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":6.331,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142248577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzyme-responsive nanoparticles: enhancing the ability of endolysins to eradicate Staphylococcus aureus biofilm† 酶响应式纳米颗粒：增强内溶菌素消除金黄色葡萄球菌生物膜的能力

IF 6.1 3区医学

Journal of Materials Chemistry B Pub Date : 2024-09-12 DOI: 10.1039/D4TB01122H

Mariana Blanco Massani, Dennis To, Susanne Meile, Mathias Schmelcher, David Gintsburg, Débora C. Coraça-Huber, Anna Seybold, Martin Loessner and Andreas Bernkop-Schnürch

{"title":"Enzyme-responsive nanoparticles: enhancing the ability of endolysins to eradicate Staphylococcus aureus biofilm†","authors":"Mariana Blanco Massani, Dennis To, Susanne Meile, Mathias Schmelcher, David Gintsburg, Débora C. Coraça-Huber, Anna Seybold, Martin Loessner and Andreas Bernkop-Schnürch","doi":"10.1039/D4TB01122H","DOIUrl":"10.1039/D4TB01122H","url":null,"abstract":"Stimuli-responsive nanomaterials show promise in eradicating Staphylococcus aureus biofilm from implants. Peptidoglycan hydrolases (PGHs) are cationic antimicrobials that can be bioengineered to improve the targeting of persisters and drug-resistant bacteria. However, these molecules can be degraded before reaching the target and/or present limited efficacy against biofilm. Therefore, there is an urgent need to improve their potency. Herein, PGH–polyphosphate nanoparticles (PGH–PP NPs) are formed by ionotropic gelation between cationic PGHs and anionic polyphosphate, with the aim of protecting PHGs and delivering them at the target site triggered by alkaline phosphatase (AP) from S. aureus biofilm. Optimized conditions for obtaining M23–PP NPs and GH15–PP NPs are presented. Size, zeta potential, and transmission electron microscopy imaging confirm the nanoscale size. The system demonstrates outstanding performance, as evidenced by a dramatic reduction in PGHs’ minimum inhibitory concentration and minimum bactericidal concentration, together with protection against proteolytic effects, storage stability, and cytotoxicity towards the Caco-2 and HeLa cell lines. Time-kill experiments show the great potential of these negatively charged delivery systems in overcoming the staphylococcal biofilm barrier. Efficacy under conditions inhibiting AP proves the enzyme-triggered delivery of PGHs. The enzyme-responsive PGH–PP NPs significantly enhance the effectiveness of PGHs against bacteria residing in biofilm, offering a promising strategy for eradicating S. aureus biofilm.","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/tb/d4tb01122h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142215164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Near-infrared light triggered photothermal therapy and enhanced photodynamic therapy with a tumor-targeting hydrogen peroxide shuttle 更正：近红外线触发的光热疗法和使用肿瘤靶向过氧化氢梭子的增强型光动力疗法

IF 6.1 3区医学

Journal of Materials Chemistry B Pub Date : 2024-09-12 DOI: 10.1039/D4TB90146K

Bing Wang, Weiming Lin, Zhengwei Mao and Changyou Gao

引用次数: 0

A chemiluminescence immunosensor for biomarker detection based on boronic acid-modified magnetic composite microspheres 基于硼酸改性磁性复合微球的生物标记物检测化学发光免疫传感器

IF 6.331 3区医学

Journal of Materials Chemistry B Pub Date : 2024-09-12 DOI: 10.1039/d4tb01582g

Xiuli Wang, Leyi He, Yaoxia Li, Jia Guo, Changchun Wang

{"title":"A chemiluminescence immunosensor for biomarker detection based on boronic acid-modified magnetic composite microspheres","authors":"Xiuli Wang, Leyi He, Yaoxia Li, Jia Guo, Changchun Wang","doi":"10.1039/d4tb01582g","DOIUrl":"https://doi.org/10.1039/d4tb01582g","url":null,"abstract":"High-sensitivity detection of biomarkers in biological samples is crucial for the early diagnosis and treatment of diseases. In this paper, a versatile and flexible immobilization technique based on the specific affinity interaction between boronic acid and cis-diol groups of antibodies was developed for biomarker detection. As a model, the boronic acid-modified immunomagnetic beads were used for facile and quick immobilization of the alpha-fetoprotein (AFP) antibody due to the specific affinity interactions. Based on this new class of immunomagnetic beads, the chemiluminescence immunosensor could efficiently detect the biomarker of AFP. Under optimal conditions, the limit of detection (LOD) is as low as 8 fM (S/N = 3), showcasing superior sensitivity and detection specificity for AFP. Subsequently, the system was successfully applied to the detection of AFP in fetal bovine serum samples, and the average recovery rate is greater than 95%. Its performance surpassed that of commercial immunomagnetic beads, showcasing the potential application of this new strategy for bioanalysis and clinical diagnosis.","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":6.331,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142248618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0