Journal of Materials Chemistry B最新文献

{"title":"Advances in three-dimensional hydrogel networks for cancer immunotherapy","authors":"Narsimha Mamidi and Amin Orash Mahmoudsalehi","doi":"10.1039/D5TB01133G","DOIUrl":"10.1039/D5TB01133G","url":null,"abstract":"<p >Cancer immunotherapies, including chimeric antigen receptor (CAR) T-cell and immune checkpoint blockade (ICB) therapy, have revolutionized oncological treatment. However, suboptimal patient response rates and immune-related adverse events continue to pose significant clinical challenges. The immunosuppressive tumor microenvironment (TME) is a critical factor influencing immunotherapy efficacy and it can impede immune cell infiltration and functionality. Three-dimensional (3D) hydrogel networks offer a promising approach to modulating the immunosuppressive characteristics of the TME by targeting and responding to its distinct physicochemical properties, such as aberrant acidity, hypoxia, elevated reactive oxygen species (ROS) levels, irregular vasculature, and a dense extracellular matrix (ECM). This review summarizes recent advances in hydrogel-based cancer immunotherapy, providing insights into refining targeted immunotherapeutic strategies to enhance anti-tumor immune responses. It explores hydrogel-based immunotherapy strategies designed to modulate these characteristics within tumors, aiming to improve the efficacy of diverse immunotherapeutic approaches. It begins by outlining the opportunities associated with hydrogel-facilitated immunotherapy. Subsequent sections describe the advantageous properties of hydrogels for cancer immunotherapy, including immune cell-based therapy, combinatorial approaches, targeted therapy, and vaccines using hydrogel matrices. We discuss how hydrogel platforms unlock previously unattainable avenues for immunotherapy. Finally, we discuss future outlooks and research directions in this rapidly evolving field.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 34","pages":" 10440-10459"},"PeriodicalIF":6.1,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144823515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipopolysaccharide-imprinted magneto-TiO2 nanoagents harness dopamine charge transfer to drive visible-light photodynamic therapy for sepsis","authors":"Jiateng Wu, Jiali Wang, Weige Dong, Yu Wan, Chungu Zhang, Ming-Yu Wu and Shun Feng","doi":"10.1039/D5TB01349F","DOIUrl":"10.1039/D5TB01349F","url":null,"abstract":"<p >Conventional TiO<small>₂</small>-based photodynamic therapy (PDT), which relies on ultraviolet (UV) activation, faces critical limitations including non-specific reactive oxygen species (ROS) generation causing collateral tissue damage, high-power density requirements risking thermal injury, and limited spatiotemporal precision due to broad-spectrum absorption. To address these challenges, we constructed a visible-light-driven nanoplatform through ligand-to-metal charge transfer (LMCT) engineering. The platform, termed LPS-MIP, integrates a polydopamine (PDA) molecular imprinting layer with a Fe<small>₃</small>O<small>₄</small>@SiO<small>₂</small>@TiO<small>₂</small> core. The PDA layer not only creates pathogen-specific recognition cavities <em>via</em> boronate affinity imprinting for selective <em>P. aeruginosa</em> binding but also establishes an LMCT pathway with TiO<small>₂</small>, shifting its activation spectrum to visible light. This innovation enables UV-free ROS generation under low-intensity white LED light (100 mW cm<small>⁻²</small>), eliminating off-target toxicity while achieving complete bacterial eradication within 120 min, with 6.6-fold higher photocurrent density than UV-activated TiO<small>₂</small>. In murine sepsis models, LPS-MIP demonstrated &gt;99% bacterial clearance in the bloodstream, suppressed hyperinflammation (TNF-α/IL-6 reduced to baseline levels), and prevented multiorgan damage, outperforming gentamicin-treated controls. The embedded Fe<small>₃</small>O<small>₄</small> core enabled rapid magnetic retrieval, reducing hepatic nanoparticle retention by 85%. By replacing UV with biocompatible visible light and confining ROS production to pathogen-binding sites, this design resolves the long-standing trade-off between antimicrobial efficacy and systemic toxicity, offering a clinically adaptable strategy for precision sepsis therapy.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 36","pages":" 11309-11317"},"PeriodicalIF":6.1,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of hydrophilic/hydrophobic diols on the properties of polyurethane hydrogels: solvent-free one-pot synthesis","authors":"Anumon V. Divakaran, Sanoop B. Nair, Shivani S. Karambe, Prakash P. Wadgaonkar, Kiran Sukumaran Nair and Manohar V. Badiger","doi":"10.1039/D5TB00928F","DOIUrl":"10.1039/D5TB00928F","url":null,"abstract":"<p >Herein, we report the design and synthesis of bio-degradable porous polyurethane hydrogels by a green, solvent-free, one-pot technique that can withstand physiological mechanical loads and aid in tissue regeneration. The hydrophilic/hydrophobic nature of the hydrogel was tuned using diols such as polycaprolactone diol (PCL) and polycarbonate diol (PCD), in combination with polyethylene glycol (PEG, MW ≈ 4000 g mol<small>⁻¹</small>), 4,4′-methylene bis(cyclohexyl isocyanate) (H<small>₁₂</small>MDI) and hexanetriol (HT), which served as crosslinking agents. The structural characterizations of the hydrogels were performed using FT-IR as well as <small>¹</small>H and <small>¹³</small>C high resolution magic angle spinning nuclear magnetic resonance (HR-MAS) spectroscopy. The utilization of various diols in the synthesis of the hydrogels enabled precise control over crystallinity, pore sizes, and customization of mechanical and degradation properties. These hydrogels exhibited tensile strength in the range of 0.22–1.48 MPa, while their compressive strength varied from 0.92 to 29.3 MPa. <em>In vitro</em> degradation profiles in the presence and absence of the enzyme Amano lipase PS revealed that the degradation process is contingent upon the specific diol present in the hydrogel. Furthermore, preliminary <em>in vitro</em> biological experiments confirmed the biocompatibility of the gels, indicating their potential as suitable substrates for drug delivery applications. This diverse library of gels can be shaped into specific forms, highlighting their promising applications as scaffolds and implants in drug delivery systems and tissue engineering.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 35","pages":" 11010-11019"},"PeriodicalIF":6.1,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144877639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel CRISPR-Cas12a-based diagnostic for rapid and highly sensitive detection of West Nile virus","authors":"Asli Erol, Dilan Celebi-Birand, Ebru Yilmaz, Ceylan Polat, Ozge Erisoz Kasap, Bulent Alten and Memed Duman","doi":"10.1039/D5TB01268F","DOIUrl":"10.1039/D5TB01268F","url":null,"abstract":"<p >Climate change is increasing the global threat of vector-borne diseases, including West Nile Virus (WNV), a significant human and animal pathogen transmitted primarily by <em>Culex</em> mosquitoes. Current WNV diagnostic methods, while including sensitive techniques like RT-PCR, have limitations in early detection, practicality, and cost-effectiveness. There is an urgent need to develop novel and more efficient strategies to address these challenges and to facilitate the surveillance and management of WNV infections and their spread. This study presents a highly specific and sensitive CRISPR-Cas12a-based detection protocol for WNV detection. Through systematic analysis of key reaction parameters (time: 0–60 min; reporter concentration: 1–80 nM, Cas12a and crRNA concentration: 5.625–90 nM; and template amount: 10<small>⁻²</small>–10<small>⁵</small> pg) and integration with reverse transcriptase recombinase polymerase amplification to enhance sensitivity through an isothermal technique, this assay demonstrates a novel strategy for the rapid detection of WNV, achieving 10 femtomolar sensitivity within one hour. Moreover, the assay retained its efficacy at different temperatures (25 °C and 37 °C) and in biological matrices containing the host (fly or human) genetic material, which supports its applicability in resource-limited settings. Therefore, the method presented here has the potential for broad application in diverse point-of-care settings for rapid diagnosis of WNV.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 36","pages":" 11318-11328"},"PeriodicalIF":6.1,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d5tb01268f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative approaches to melanoma treatment: a spotlight on stimuli-responsive biomaterials","authors":"Ashleigh Tinotenda Chitakunye, Odinaka Cassandra Ezekiel, Qin Liu, Shihui Zhang, Qin Zhu and Lin Cai","doi":"10.1039/D4TB01504E","DOIUrl":"10.1039/D4TB01504E","url":null,"abstract":"<p >Melanoma presents as an increasingly prevalent and intricate skin cancer, characterized by a complex tumor microenvironment that features various mutations and the activation of melanogenesis pathways. Dynamic changes within this microenvironment, including increased ROS levels, acidity, and enzyme expression, specifically upregulation of glutaryl-CoA dehydrogenase and MMP2, amongst other enzymes, further contribute to its complexity. Despite advancements in melanoma treatment and FDA approval of therapies targeting specific pathways and employing immune checkpoint inhibitors, challenges persist in melanoma treatment, particularly in optimizing drug delivery and navigating the intricate tumor microenvironment. Recent research has increasingly focused on integrating biomaterials into melanoma treatment, yielding promising results. These biomaterials find application in melanoma diagnosis, treatment, and imaging. Studies have sparked interest in uncovering the therapeutic potential of stimuli-responsive biomaterials. pH-responsive systems offer the prospect of targeted drug release in the acidic tumor microenvironment. Meanwhile, light- and temperature-responsive materials offer spatiotemporal control, aiding melanoma death processes such as necroptosis, apoptosis, and necrosis. Biomaterials responsive to ROS and enzymes address the intricacies of melanoma biology, enhancing treatment specificity. Additionally, multiple stimuli-responsive platforms present a holistic approach for heightened therapeutic efficacy. This review navigates the intricate terrain of melanoma treatment, addressing current therapy limitations and envisioning a future where functionalized biomaterials are pivotal in more effective and targeted interventions. We evaluate multifaceted approaches harnessing distinct biological, physical, and chemical stimuli, and their synergistic combinations to enhance drug delivery precision and other mechanisms in melanoma.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 36","pages":" 11102-11125"},"PeriodicalIF":6.1,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel biomaterial derived from the skin secretion of Andrias davidianus for dentinal tubule occlusion†","authors":"Yongxiang Zeng, Lin Yang, Na Wu, Hong Chen, Ximu Zhang and Deqin Yang","doi":"10.1039/D5TB00823A","DOIUrl":"10.1039/D5TB00823A","url":null,"abstract":"<p >Dentin hypersensitivity is a common challenge for dentists and patients. Occluding dentinal tubules through dentin remineralization is considered an alternative therapeutic approach to manage dentin hypersensitivity. The skin secretion of <em>Andrias davidianus</em> (SSAD) contains various binding functional groups. In this study, SSAD hydrolysate was prepared with tris(2-carboxyethyl)phosphine reduction. Its binding ability to acid-etched dentin, as well as its efficacy in dentin remineralization and tubule occlusion were evaluated. The results demonstrated that SSAD hydrolysate could easily and firmly bind to the surface of collagen fibrils. This hydrolysate could stabilize calcium and phosphorus ions in an amorphous state and induce the accumulation of apatite on the dentin surface. After 4 weeks of incubation in artificial saliva, dentinal tubules treated with 0.5 mg mL<small>⁻¹</small> SSAD hydrolysate were evidently sealed by regenerated minerals. The deposited minerals were observed deep within the dentinal tubules (up to 30 ± 5 μm). X-Ray diffraction and energy-dispersive X-ray analyses further confirmed that the regenerated minerals were mainly hydroxyapatite. Sequentially, stable occlusion after acid challenge and significant reduction in dentin permeability were achieved. The microhardness value of the remineralized dentin treated with SSAD hydrolysate was significantly increased. In addition, the proliferation, migration, mineralization, and odontogenic differentiation of human dental pulp stem cells were promoted. Animal experiments indicated that SSAD hydrolysate could also occlude dentinal tubules in the oral environment. In conclusion, our work provides an experimental basis for the potential use of SSAD hydrolysate for treating dentin hypersensitivity.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 33","pages":" 10300-10313"},"PeriodicalIF":6.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d5tb00823a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144762874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-functional F127-grafted vitamin E succinate-modified liposomes for enhancing glioma therapy","authors":"Yingchong Chen, Wenhui Hu, Lizhen Huang, Yuling Liu, Fang Li, Haibin Zou and Pengfei Yue","doi":"10.1039/D5TB01101A","DOIUrl":"10.1039/D5TB01101A","url":null,"abstract":"<p >In glioma treatment, the poor penetration of therapeutic drugs through the blood–brain barrier (BBB) to the tumor region, along with the intrinsic resistance of glioma cells <em>via</em> multiple survival mechanisms, remain major challenges. Liposomes are a popular choice for glioma therapy due to their ability to help drugs penetrate the BBB. However, unmodified liposomes have drawbacks, such as poor stability, low drug entrapment efficacy, and rapid removal from circulation, leading to reduced drug absorption at the lesion sites. To address these issues, a novel F127-grafted vitamin E succinate (F127–VES) was designed to prepare multifunctional modified liposomes for delivery of harmine (HAR). Compared to conventional liposomes (Ordinary-Lip), F127–VES-Lip shows greater potential in enhancing the anti-glioma effect of HAR. This is attributed to several key features: Firstly, Pluronic can enhance the transport across the blood–brain barrier. Secondly, the presence of F127 in the formulation extends the circulation time of the drug in the bloodstream. Finally, the combination of F127 and VES in F127–VES-Lip can induce tumor cell apoptosis. <em>In vitro</em> and <em>in vivo</em> results confirmed that the F127–VES-Lip could significantly elevate cellular uptake, promote apoptosis and increase the antitumor effect for C6 glioma cells. The F127–VES-Lip is a promising nanoformulation to enhance the effect of HAR for glioma treatment.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 33","pages":" 10286-10299"},"PeriodicalIF":6.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144762876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanical strain-regulated hydrogel biodegradation for biological scaffolds with programmable lifetime","authors":"Akhiri Zannat, Adolfo Lopez, Yijie Cheng, Jie Ma, Mitchell Delemeester, Shaoting Lin, Krishnamurthy Jayaraman and Xinyue Liu","doi":"10.1039/D5TB01056J","DOIUrl":"10.1039/D5TB01056J","url":null,"abstract":"<p >Precise control over hydrogel biodegradation kinetics is of importance for drug delivery and tissue engineering. However, existing strategies usually rely on fixed material chemistries and offer limited tunability once implanted. Here, we introduce mechanical strain as a programmable cue to regulate hydrogel degradation <em>in situ</em>. Using peptide-crosslinked tetra-PEG hydrogels and proteinase K as a model system, we developed a real-time stress-monitoring platform to quantitatively study strain-dependent enzymatic degradation. Time-resolved measurements reveal that mechanical strain significantly accelerates degradation by simultaneously enhancing diffusion and reaction rates. For thick hydrogel samples, uniaxial stretching significantly reduces the degradation time by four-fold from 7.6 hours (undeformed) to 1.9 hours (stretched), shifting the process from slow, surface-limited to rapid, volumetric degradation. A multiscale theoretical model that we developed identifies three synergistic effects of mechanical strain: reduced diffusion path due to geometric thinning, increased network mesh size for enhanced enzyme penetration, and elevated chain tension that promotes bond cleavage. These findings establish mechanical loading as a universal tool in biological systems to dynamically modulate hydrogel lifetimes, offering new opportunities for programmable drug release and scaffold-guided tissue remodeling.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 34","pages":" 10485-10499"},"PeriodicalIF":6.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d5tb01056j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144805522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional Fe3O4 mesocrystals for cancer therapy: integrating hyperthermia and targeted drug delivery","authors":"Akash Marsalin, Nishakavya Saravanan, Anandhakumar Sundaramurthy, Satish S. Phalake, Vishwajeet M. Khot and Rajaboopathi Mani","doi":"10.1039/D5TB00282F","DOIUrl":"10.1039/D5TB00282F","url":null,"abstract":"<p >Mesocrystals with hierarchical architecture and crystallographically aligned nanoparticles hold immense potential for advanced applications in catalysis, energy storage and biomedicine. However, challenges arise for biomedical applications due to their surfactant-controlled growth, lack of understanding of magnetic mesocrystals and their dopant effect. Herein, we report a facile, additive-free solvothermal synthesis of Fe<small>₃</small>O<small>₄</small> mesocrystals (∼205 nm) and investigate their morphological evolution by correlating the structural changes with respect to magnetic properties. The Fe<small>₃</small>O<small>₄</small> mesocrystals exhibit a high saturation magnetization of 87 emu g<small>⁻¹</small>, surpassing that of conventional nanoparticles (55.29 emu g<small>⁻¹</small>) suitable for magnetic hyperthermia. A therapeutic temperature of 42 °C was reached at 5 and 10 mg mL<small>⁻¹</small> under applied fields of 20 and 26.7 kA m<small>⁻¹</small> in water and 2% agar media within the clinical safety limit. Furthermore, they exhibit an excellent drug encapsulation efficiency of 41.09% for paclitaxel (PTX) drugs, significantly outperforming that of the nanoparticles (19.4%), which is attributed to the internal voids of mesocrystals, nanoparticle building units and hierarchical structures with release profiles of 28% and 41% at pH 7.4 and 5.5, respectively. <em>In vitro</em> studies reveal 82% biocompatibility with L-929 fibroblast cells and 60% cell viability against HCT 116 colon cancer cells at 1 mg mL<small>⁻¹</small>. At this concentration, Fe<small>₃</small>O<small>₄</small> mesocrystals embedded with PTX show a 95% reduction in cancer cell viability. We also probed the structural characteristics using XRD, Raman, FT-IR, SEM, TEM and XPS analyses. By integrating magnetic hyperthermia with pH-dependent drug release, this work establishes Fe<small>₃</small>O<small>₄</small> mesocrystals as a dual-functional platform for targeted cancer therapy, offering a transformative approach to overcome the limitations in nanomedicine.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 34","pages":" 10635-10647"},"PeriodicalIF":6.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144857219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrospun chitosan nanofibers for regenerative wound healing: from molecular design to functional scaffolds","authors":"Devika Tripathi, P. S. Rajinikanth and Prashant Pandey","doi":"10.1039/D5TB01405K","DOIUrl":"10.1039/D5TB01405K","url":null,"abstract":"<p >The management of acute and chronic wounds remains a clinical challenge due to infection, delayed re-epithelialization, and impaired angiogenesis. Electrospun nanofibrous scaffolds have emerged as promising biomaterials, offering high surface area-to-volume ratios, tunable porosity, and ECM-like architectures. Chitosan, derived from chitin, is a biocompatible, biodegradable, and antimicrobial natural polymer ideally suited for wound healing. Electrospun chitosan nanofibres support cellular proliferation, modulate inflammation, and promote tissue regeneration. This review examines recent advances in the fabrication and biomedical applications of electrospun chitosan-based nanofibres for wound healing. Key electrospinning parameters, such as polymer concentration, molecular weight, solution viscosity, and applied voltage, are discussed. Various electrospinning strategies, including blend, coaxial, emulsion, and multilayer methods, are explored for encapsulating therapeutic agents, controlling drug release, and enhancing scaffold performance. The influence of polymer blends, crosslinking methods, and solvent systems on nanofibre morphology and mechanical integrity is also examined. Significantly, this work bridges materials design with clinical functionality, offering a roadmap for translating molecular-level chitosan modifications and nanostructure control into precision medicine. Beyond wound healing, the fabrication strategies and design principles discussed herein hold broad relevance for the fields of materials science and biomedical engineering, particularly in developing next-generation bioresponsive materials, tissue scaffolds, and drug delivery systems. As the field evolves, electrospun chitosan nanofibres are poised to play a pivotal role in advancing smart, adaptive, and regenerative biomaterials for diverse therapeutic applications.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 35","pages":" 10743-10779"},"PeriodicalIF":6.1,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144983620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}