Journal of Materials Chemistry B最新文献

{"title":"Mixed-valence vanadium-doped mesoporous bioactive glass for treatment of tumor-associated bone defects†","authors":"Xin Liu, Peng Zhang, Mengjie Xu, Zihao Zhao, Xing Yin, Ximing Pu, Juan Wang, Xiaoming Liao, Zhongbing Huang, Shunze Cao and Guangfu Yin","doi":"10.1039/D4TB02290D","DOIUrl":"10.1039/D4TB02290D","url":null,"abstract":"<p >Vanadium is a bioactive trace element with variable valence. Its pentavalent form has been confirmed to be capable of predominantly regulating the early and mid-stage osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) without tumor inhibition, while its tetravalent form exhibits tumor inhibition but only primarily modulates late osteogenic differentiation and angiogenesis. In this study, a multifunctional bone tissue scaffold consisting of mixed-valence vanadium-doped mesoporous bioactive glass and poly(lactic-<em>co</em>-glycolic acid) (V(<small>IV</small>/<small>V</small>)-MBG/PLGA) was developed to simultaneously inhibit the recurrence of osteosarcoma and promote the regeneration of operative bone defects. The <em>in vitro</em> results showed that the V(<small>IV</small>) and V(<small>V</small>) species could be sustainably released from V(<small>IV</small>/<small>V</small>)-MBG and complementarily enhance the proliferation, osteogenic differentiation, and mineralization of BMSCs by activating multiple signaling pathways throughout the whole osteogenesis process. More importantly, the co-existence of mixed-valent vanadium species was able to continuously stimulate the generation of excessive ROS and the depletion of GSH by synergistically supplying an appropriate ratio of V(<small>IV</small>) and V(<small>V</small>) to thermodynamically and kinetically maintain the stable self-circulation of the valence state alteration, thus inducing UMR-106 cell death. In a rat model, V(<small>IV</small>/<small>V</small>)-MBG/PLGA scaffolds effectively suppressed tumor invasion and promoted bone regeneration. These results suggest that V(<small>IV</small>/<small>V</small>)-MBG/PLGA scaffolds are a promising strategy for treating tumor-associated bone defects, offering dual tumor inhibition and bone regeneration.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 9","pages":" 3138-3160"},"PeriodicalIF":6.1,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an AI-derived, non-invasive, label-free 3D-printed microfluidic SERS biosensor platform utilizing Cu@Ag/carbon nanofibers for the detection of salivary biomarkers in mass screening of oral cancer†","authors":"Navami Sunil, Rajesh Unnathpadi, Rajkumar Kottayasamy Seenivasagam, T. Abhijith, R. Latha, Shina Sheen and Biji Pullithadathil","doi":"10.1039/D4TB02766C","DOIUrl":"10.1039/D4TB02766C","url":null,"abstract":"<p >Developing a non-invasive and reliable tool for the highly sensitive detection of oral cancer is essential for its mass screening and early diagnosis, and improving treatment efficacy. Herein, we utilized a label-free surface enhanced Raman spectroscopy (SERS)-based biosensor composed of Cu@Ag core–shell nanoparticle anchored carbon nanofibers (Cu@Ag/CNFs) for highly sensitive salivary biomarker detection in oral cancer mass screening. This SERS substrate provided a Raman signal enhancement of up to 10<small>⁷</small> and a detection limit as low as 10<small>⁻¹²</small> M for rhodamine 6G molecules. Finite-difference time-domain (FDTD) simulation studies on Cu@Ag/CNFs indicated an <em>E</em>-field intensity enhancement factor (|<em>E</em>|<small>²</small>/|<em>E</em><small>₀</small>|<small>²</small>) of 250 at the plasmonic hotspot induced between two adjacent Cu@Ag nanoparticles. The interaction of this strong <em>E</em>-field along with the chemical enhancement effects was responsible for such huge enhancement in the Raman signals. To realize the real capability of the developed biosensor in practical scenarios, it was further utilized for the detection of oral cancer biomarkers such as nitrate, nitrite, thiocyanate, proteins, and amino acids with a micro-molar concentration in saliva samples. The integration of SERS substrates with a 3D-printed 12-channel microfluidic platform significantly enhanced the reproducibility and statistical robustness of the analytical process. Moreover, AI-driven techniques were employed to improve the diagnostic accuracy in differentiating the salivary profiles of oral cancer patients (<em>n</em><small>₁</small> = 56) from those of healthy controls (<em>n</em><small>₂</small> = 60). Principal component analysis (PCA) was utilized for dimensionality reduction, followed by classification using a random forest (RF) algorithm, yielding a robust classification accuracy of 87.5%, with a specificity of 92% and sensitivity of 88%. These experimental and theoretical findings emphasize the real-world functionality of the present non-invasive diagnostic tool in paving the way for more accurate and early-stage detection of oral cancer in clinical settings.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 10","pages":" 3405-3419"},"PeriodicalIF":6.1,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143401000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Phosphatidylserine-functionalized liposomes-in-microgels for delivering genistein to effectively treat ulcerative colitis","authors":"Huijia Yan, Yanfei Li, Sihui Li, Di Wu, Yu Xu and Jiangning Hu","doi":"10.1039/D5TB90026C","DOIUrl":"10.1039/D5TB90026C","url":null,"abstract":"<p >Correction for ‘Phosphatidylserine-functionalized liposomes-in-microgels for delivering genistein to effectively treat ulcerative colitis’ by Huijia Yan <em>et al.</em>, <em>J. Mater. Chem. B</em>, 2023, <strong>11</strong>, 10404–10417, https://doi.org/10.1039/D3TB00812F.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 8","pages":" 2871-2872"},"PeriodicalIF":6.1,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d5tb90026c?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143191581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of the diabetic immune microenvironment by metformin-loaded strontium-doped mesoporous bioactive glass facilitates bone regeneration†","authors":"Qing Ye, Min Zhang, Qianrui Li, Lingling Jia, Yuan Gao, He Yuan and Jiyao Li","doi":"10.1039/D4TB01778A","DOIUrl":"10.1039/D4TB01778A","url":null,"abstract":"<p >Chronic inflammation and oxidative stress in the diabetic microenvironment often hinder the healing of bone defects. Metformin (MET), the first-line diabetes medication, was shown to alter macrophage polarization toward an anti-inflammatory M2 phenotype, while simultaneously suppressing excessive reactive oxygen species (ROS) generation. Strontium-doped mesoporous bioactive glasses (SrMBG) also showed promising benefits in promoting bone regeneration. Aiming to improve the diabetic immune microenvironment within bone defects, in this study we loaded SrMBG mesopores with increasing amounts of MET. MET-loaded SrMBG (MET/SrMBG) extracts promoted macrophage differentiation toward the M2 phenotype and reduced ROS production <em>in vitro.</em> The possibility of facilitating osteogenic differentiation of bone marrow stromal cells (BMSCs) <em>in vivo</em> led us to develop 3D-printed MET/SrMBG scaffolds. Experiments utilizing a critical-size calvarium defect model in diabetic rats confirmed that implanting the MET/SrMBG scaffold enhanced bone repair owing to the effects of MET regulating M2 type macrophage polarization and mitigating oxidative stress to improve the inflammatory microenvironment.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 9","pages":" 3114-3127"},"PeriodicalIF":6.1,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143191335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A phlorotannin nanoparticle–hydrogel composite for enhanced oral delivery and treatment of ulcerative colitis†","authors":"Wen Jiang, Yu Xu, Xin-Chuang Wang, Di Wu, Yi-Nan Du and Jiang-Ning Hu","doi":"10.1039/D4TB01811G","DOIUrl":"10.1039/D4TB01811G","url":null,"abstract":"<p >Designing innovative strategies for oral delivery of active compounds is particularly promising for the treatment of intestinal diseases such as ulcerative colitis (UC). However, obstacles like poor therapeutic efficacy, low bioavailability, and limited biocompatibility need to be addressed. Here, <em>via</em> the Schiff base reaction, we developed nanoparticles based on PT (PT NPs) and incorporated them into ascorbate palmitate hydrogels (AP-Gel) to create nanoparticle–hydrogel composites (PT NPs–Gel). <em>In vitro</em> studies showed that PT NPs–Gel reduced pro-inflammatory cytokines (NO, iNOS, TNF-α, and IL-1β), increased the anti-inflammatory cytokine IL-10, and enhanced antioxidant enzyme activities (SOD and CAT) with effect. In the DSS-induced UC mouse model, PT NPs–Gel significantly alleviated UC symptoms, improved the length of colon, and lowered the disease activity index (DAI) score. Histological analysis indicated that PT NPs–Gel protected the colonic epithelial barrier and reduced inflammation. The PT NPs–Gel formulation utilizes the biological properties of the hydrogel carrier to improve the bioavailability of active compounds and demonstrates effective anti-inflammatory and antioxidant properties, making it a promising oral delivery system for the treatment of UC and potentially other inflammatory bowel diseases.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 9","pages":" 3080-3093"},"PeriodicalIF":6.1,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143124033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptide-functionalized nanocapsules for targeted inhibition of β2-microglobulin amyloid aggregation†","authors":"Lin Tang, Miao Sun, Junnan Chen, Qiong Dai, Song Xue, Chaoyong Liu and Ming Zhang","doi":"10.1039/D4TB01347F","DOIUrl":"10.1039/D4TB01347F","url":null,"abstract":"<p >Dialysis-related amyloidosis (DRA) is a severe complication in patients undergoing long-term dialysis, primarily driven by the deposition of β2-microglobulin (β2m) amyloid fibrils. The effective sequestration and removal of β2m from the bloodstream represent key therapeutic strategies for managing DRA. In this study, we developed a β2m-binding peptide (KDWSFYILAHTEF, denoted as CF)-functionalized nanocomposite (NC-CF), consisting of a protein nanocapsule surface modified with CF peptides to enable specific β2m binding. NC-CF effectively modulates β2m aggregation, transforming slender fibrils into larger clumps while providing steric hindrance to prevent further aggregation. With a high adsorption capacity, 1 μg of NC-CF can adsorb approximately 1 μg of β2m during dialysis, highlighting its potential as an efficient adsorbent for <em>in vitro</em> β2m removal. Furthermore, NC-CF exhibits excellent biocompatibility and significantly mitigates β2m aggregate-induced cytotoxicity, achieving a cell protection rate exceeding 70%. These findings suggest that NC-CF holds great promise as a cytoprotective agent and a nanoinhibitor of β2m aggregation <em>in vivo</em>. Overall, NC-CF offers a novel and effective approach for alleviating DRA by simultaneously removing β2m and safeguarding cells against amyloid-induced toxicity.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 10","pages":" 3319-3324"},"PeriodicalIF":6.1,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143384515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A piezoelectric hydrogel containing bismuth sulfide with cationic vacancies with enhanced sonodynamic/nanozyme activity for synergistically killing bacteria and boosting osteoblast differentiation†","authors":"Xiaowen Xi, Susu Ma, Ping Sun, Zhitao Hu, Jie Wei and Yunfei Niu","doi":"10.1039/D4TB02693D","DOIUrl":"10.1039/D4TB02693D","url":null,"abstract":"<p >A piezoelectric nanozyme is a novel biomaterial with the integration of piezoelectricity and nanozyme activity that has the capability of killing bacteria and promoting cell responses under a mechanical stimulus and exhibits great prospects in tissue regeneration. Herein, a piezoelectric nanozyme of bismuth sulfide (BS) with cationic vacancies (VBS) was synthesized, which exhibits enhanced piezoelectricity and nanozyme activities compared with BS. Moreover, a piezoelectric hydrogel of VBS and phenylboronic acid grafted sodium alginate-arginine (VBS-PSA) was prepared. Triggered by ultrasound (US) with high power (&gt;0.5 W cm<small>⁻²</small>), VBS-PSA produces a large amount of reactive oxygen species (ROS) through both piezoelectricity-enhanced sonodynamic efficiency and peroxidase-like (POD-like) activity, thereby displaying the powerful antibacterial capability. However, under low-power US (≤0.5 W cm<small>⁻²</small>), the piezoelectric effect of VBS-PSA generates electrical signals that significantly stimulate the osteoblast responses (proliferation and osteoblast differentiation) and enhance catalase-like (CAT-like) activity for scavengers of ROS and generation of oxygen, thereby creating a favorable microenvironment for cell growth. Our study presents a novel strategy to apply the piezoelectric effect of hydrogels for enhancing sonodynamic efficiency and nanozyme activities that synergistically kill bacteria and stimulate osteoblast responses. The piezoelectric hydrogel would have great potential for the repair of infected bone defects.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 10","pages":" 3420-3436"},"PeriodicalIF":6.1,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-functional guanosine-based hydrogel: high-efficiency protection in radiation-induced oral mucositis†","authors":"Zihan Ding, Xiaopei Hu, Wenhui Liang, Shuhao Zheng, Xiaobo Luo and Hang Zhao","doi":"10.1039/D4TB02380C","DOIUrl":"10.1039/D4TB02380C","url":null,"abstract":"<p >Radiation-induced oral mucositis (RIOM) is the most common adverse effect experienced by cancer patients following radiotherapy; however, effective clinical treatments remain insufficiently recognized. In this study, a guanosine-polyvinyl alcohol (G-PVA) supramolecular hydrogel was developed using a one-pot synthesis method. The G-PVA hydrogel demonstrated remarkable wet adhesion properties measuring 74.16 kPa (±3.53 kPa), biocompatibility and shape adaptability, making it suitable for the dynamic conditions of the oral cavity. Under radiation exposure, the G-PVA hydrogel not only mitigated oxidative stress but also provided effective protection to cells against direct damage, as shown by the decrease in the number of γ-H2AX foci from 79.81% (±1.33%) to 5.70% (±0.88%) and 17.12% (±3.44%), respectively. <em>In vivo</em> investigations further validated that the G-PVA hydrogel significantly reduced RIOM, with a decrease in the relative ulcerative area by 52.23% in comparison to the IR group. This dual-functional supramolecular hydrogel may represent a promising new clinical strategy for the prevention of RIOM in the future.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 9","pages":" 3039-3048"},"PeriodicalIF":6.1,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-layered electrode constructs for neural tissue engineering†","authors":"Marjolaine Boulingre, Mateusz Chodkowski, Roberto Portillo Lara, Aaron Lee, Josef Goding and Rylie A. Green","doi":"10.1039/D4TB02651A","DOIUrl":"10.1039/D4TB02651A","url":null,"abstract":"<p >Although neural tissue engineering holds great therapeutic potential for multiple clinical applications, one important challenge is the development of scaffolds that provide cues required for neural tissue development. To achieve this, biomaterial systems can be leveraged to present appropriate biological, mechanical, topographical and electrical cues that could direct cell fate. In this study, a multi-layered electrode construct was engineered to be used as a platform for 3D cell encapsulation for <em>in vitro</em> applications. The first layer is a conductive hydrogel coating, that improves electrical conductivity from the underlying platinum electrode. The second layer is a biosynthetic hydrogel, specifically tailored to support neural development. This layered electrode construct was electrochemically characterised, and a numerical model was applied to study electrical stimuli reaching the biosynthetic hydrogel layer. The construct was shown to effectively support the growth and proliferation of encapsulated astrocytes within the biosynthetic layer, while the numerical model will enable computational experimentation for benchmarking and study validation. This highly versatile system represents a robust tool to study the influence of electrical stimuli on neural fate, as well as investigating the development of biohybrid interfaces <em>in vitro</em>.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 10","pages":" 3390-3404"},"PeriodicalIF":6.1,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d4tb02651a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143401003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterogeneous biocatalysis by magnetic nanoparticle immobilized biomass-degrading enzymes derived from microbial cultures†","authors":"Mani Gupta, Arghya Bandyopadhyay, Sushant Kumar Sinha, Suksham, Abhisek Samanta, Surajit Mondal, Sayan Bhattacharyya and Supratim Datta","doi":"10.1039/D4TB02011A","DOIUrl":"10.1039/D4TB02011A","url":null,"abstract":"<p >Recombinant enzymes have become increasingly popular and are frequently used as environmentally safe biocatalysts due to their wide range of applications and high specificity. Purifying these enzymes from the host cells, media, and other contaminants is essential for their characterization and applications. The widely utilized method for protein purification by nickel–nitrilotriacetic acid (Ni–NTA) resin-based affinity chromatography is a time-consuming, labor-intensive, and resource-demanding technique. In this study, we synthesized NTA–Ni@Fe<small>₃</small>O<small>₄</small> nanoparticles (NPs) to capture enzymes from cell lysates and microbial culture media and developed a model system to show the efficacy of immobilizing and recycling biomass-degrading enzymes known as cellulases. Cellulases, which play an important role in biomass degradation and biofuel production, were baited with NTA–Ni@Fe<small>₃</small>O<small>₄</small> NPs and purified in a single step. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis indicates efficient purification of the immobilized enzymes on the NPs from the cell lysate and extracellular media. Additionally, we successfully showed that cellulase-immobilized NTA–Ni@Fe<small>₃</small>O<small>₄</small>NPs can serve as a heterogeneous catalyst for the hydrolysis of <em>p</em>-nitrophenyl-β-<small>D</small>-glucopyranoside (<em>p</em>NPGlc) and carboxymethylcellulose (CMC). The NTA–Ni@Fe<small>₃</small>O<small>₄</small> NPs immobilized with enzymes showed recyclability for up to five cycles. The applications of this methodology may be extended to various industries requiring efficient enzyme purification and recycling for promising advancements in biotechnology and sustainable biomanufacturing processes.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 11","pages":" 3644-3652"},"PeriodicalIF":6.1,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}