Journal of Materials Chemistry B最新文献

{"title":"Salicylidene-based dual-responsive ‘turn on’ fluorometric chemosensors for the selective detection of Zn2+, Al3+ and F− ions: theoretical investigation and applications in the live cell imaging of zebrafish larvae and molecular logic gate operation†","authors":"Abbas Khaja Raees Ahmed, Ramalingam Gajendhiran, Sivaraj Mithra, Seepoo Abdul Majeed, Azeez Sait Sahul Hameed, Rajakkani Paulpandiyan, Subbaiah Maniyammai, Gurusamy Thangavelu Senthil Andavan, MohamedHanifa NizamMohideen and Aziz Kalilur Rahiman","doi":"10.1039/D4TB01356E","DOIUrl":"10.1039/D4TB01356E","url":null,"abstract":"<p >Four salicylidene-based dual-responsive chemosensors 1,5-bis(5-bromosalicylaldehyde)carbohydrazone (R1), 1,5-bis(5-bromosalicylaldehyde)thiocarbohydrazone (R2), 1,5-bis(3-ethoxysalicylaldehyde)carbohydrazone (R3) and 1,5-bis(3-ethoxysalicylaldehyde)thiocarbohydrazone (R4) were synthesized and characterized. The molecular structures of R1 and R3 were confirmed by single crystal X-ray diffraction technique, which crystallized in the orthorhombic <em>Pbcn</em> and monoclinic <em>P</em>2<small><sub>1</sub></small>/<em>n</em> space groups, respectively. The chemosensor molecules were investigated for their recognition properties against the selected cations (K<small><sup>+</sup></small>, Ca<small><sup>2+</sup></small>, Mn<small><sup>2+</sup></small>, Co<small><sup>2+</sup></small>, Ni<small><sup>2+</sup></small>, Cu<small><sup>2+</sup></small>, Zn<small><sup>2+</sup></small>, Fe<small><sup>3+</sup></small> and Al<small><sup>3+</sup></small>) and anions (F<small><sup>−</sup></small>, Cl<small><sup>−</sup></small>, Br<small><sup>−</sup></small>, I<small><sup>−</sup></small>, HSO<small><sub>4</sub></small><small><sup>−</sup></small>, H<small><sub>2</sub></small>PO<small><sub>4</sub></small><small><sup>−</sup></small>, ClO<small><sub>4</sub></small><small><sup>−</sup></small>, N<small><sub>3</sub></small><small><sup>−</sup></small> and NO<small><sub>3</sub></small><small><sup>−</sup></small>) by colorimetry, absorption spectroscopy, fluorescence spectroscopy, <small><sup>1</sup></small>H NMR spectroscopy and theoretical studies. The sensor molecules showed colorimetric responses for the Co<small><sup>2+</sup></small>, Ni<small><sup>2+</sup></small>, Cu<small><sup>2+</sup></small> and Fe<small><sup>3+</sup></small> cations and the F<small><sup>−</sup></small> anion. Interestingly, the Zn<small><sup>2+</sup></small> and Al<small><sup>3+</sup></small> cations showed only the 'turn on' fluorometric response, whereas the F<small><sup>−</sup></small> anion showed both colorimetric and fluorometric responses. The binding constants were determined using the Benesi–Hildebrand (B–H) equation from the fluorescence titrations and found to be higher for R3 towards the Al<small><sup>3+</sup></small> cation (2.03 × 10<small><sup>6</sup></small> M<small><sup>−1</sup></small>) with a low limit of detection (1.79 μM) and for R4 towards the F<small><sup>−</sup></small> anion (5.13 × 10<small><sup>5</sup></small> M<small><sup>−1</sup></small>) with a low limit of detection (5.23 μM). The chemosensors established 1 : 2 and 1 : 1 binding stoichiometries with the sensed cations and anion, respectively, as confirmed by Job's plots. The computational studies show a lower band gap of HOMO–LUMO when the chemosensors bind with the sensed inorganic ions compared to the free chemosensors. Furthermore, the observed fluorescent behaviour of the Zn<small><sup>2+</sup></small> and Al<small><sup>3+</sup></small> cations have motivated us to investigate the practical applications in the","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 2","pages":" 622-641"},"PeriodicalIF":6.1,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142735428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-functional PCN-242 (Fe2Co) MOF for sensitive bacterial endotoxin detection†","authors":"Sivasankar Kulandaivel, Yung-Kang Lu, Chia-Her Lin and Yi-Chun Yeh","doi":"10.1039/D4TB01944J","DOIUrl":"10.1039/D4TB01944J","url":null,"abstract":"<p >Endotoxin detection is paramount for monitoring bacterial contamination in food, pharmaceuticals, and clinical diagnostics. The limulus amebocyte lysate (LAL) test, which relies on horseshoe crab blood, has long been the gold standard for endotoxin detection. However, the widespread adoption of this method is constrained by ethical concerns and the high costs associated with harvesting endangered species. Although nanozyme-based colorimetric methods present a more cost-effective and straightforward alternative, their application is limited by suboptimal selectivity and sensitivity. In this study, we report the synthesis and rigorous characterization of the bimetallic PCN-242 (Fe<small>₂</small>Co) metal–organic framework (MOF), synthesized using 2-amino terephthalic acid and a pre-synthesized [Fe<small>₂</small>Co(μ<small>₃</small>-O)(CH<small>₃</small>COO)<small>₆</small>] cluster. Steady-state kinetic analyses revealed that PCN-242 (Fe<small>₂</small>Co) MOF exhibits a significantly higher affinity for hydrogen peroxide (H<small>₂</small>O<small>₂</small>) compared to horseradish peroxidase (HRP) and other iron-based MOFs. The development of a PCN-242 (Fe<small>₂</small>Co)-based colorimetric sensor demonstrated a low limit of detection (LOD) of 1.36 μg mL<small>⁻¹</small> for endotoxins, with excellent selectivity and reproducibility, thereby enabling effective detection of bacterial endotoxins. Recognizing the potential of the PCN-242 (Fe<small>₂</small>Co) MOF beyond endotoxin detection, we explored its utility in glucose biosensing. Moreover, incorporating glucose oxidase (GOx) into the PCN-242 (Fe<small>₂</small>Co) MOF framework further enhanced its peroxidase-like catalytic activity. This integration enabled sensitive glucose detection, achieving LODs of 4.24 μM for glucose and 2.2 μM for H<small>₂</small>O<small>₂</small> within a linear range of 1 to 150 μM. The dual functionality of PCN-242 (Fe<small>₂</small>Co) MOF as a peroxidase mimic and biosensor platform highlights its potential for advanced catalytic and diagnostic applications, offering a versatile and ethical alternative to conventional methods.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 1","pages":" 151-159"},"PeriodicalIF":6.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bimetallic doped carbon dot nanozymes for enhanced sonodynamic and nanocatalytic therapy†","authors":"Yandong Huang, Lanting Jia, Shiqi Zhang, Lang Yan and Lei Li","doi":"10.1039/D4TB01916D","DOIUrl":"10.1039/D4TB01916D","url":null,"abstract":"<p >Conventional inorganic semiconductors are not suitable for acting as nanozymes or sonosensitizers for <em>in vivo</em> therapeutic nanomedicine owing to the lack of excellent biocompatibility. Biocompatible carbon dots (CDs) exhibit a variety of biological activities due to their adjustable size and surface chemical modification; however, the simultaneous sonodynamic activity and multiple enzyme-mimicking catalytic activity of a single CD have not been reported. Herein, we report the development of bimetallic doped CDs as a high-efficiency nanozyme and sonosensitizer for enhanced sonodynamic therapy (SDT) and nanocatalytic therapy (NCT). By selecting metal–organic complexes like EDTA–FeNa as the carbon source, we ensure that the coordination environments of metal atoms are preserved throughout the low-temperature calcination process. Compared with the single metal doped CDs including Fe–CDs or Ni–CDs, the obtained Fe and Ni co-doped CDs (Fe–Ni–CDs) not only exhibit enhanced sonodynamic activity owing to the decreased bandgap, but also possess augmented dual enzyme-mimicking catalytic activities due to the synergistic effect of bimetallic ions. The Fe–Ni–CD-mediated cascade amplification of ROS generation could lead to the production of <small>¹</small>O<small>₂</small> and O<small>₂</small>˙<small>⁻</small> through SDT, the generation of ˙OH through POD-mimicking catalytic activity, and the provision of more O<small>₂</small> for SDT through CAT-mimicking catalytic activity. Through the integrated multifunctionality of Fe–Ni–CDs, we successfully enhanced the effectiveness of antitumor treatment with a single drug injection and a single US irradiation for enhanced SDT and NCT. This work provides a distinct paradigm of endowing CDs with sonodynamic and multiple enzyme-mimicking catalytic activities for enhanced SDT and NCT through bimetallic ion doping.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 2","pages":" 588-598"},"PeriodicalIF":6.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142690058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in polymer-based thin-film electrodes for ECoG applications","authors":"Zhengchen Xiang, Liangtao Yang, Bin Yu, Qi Zeng, Tao Huang, Shuo Shi, Hao Yu, Yi Zhang, Jinglong Wu and Meifang Zhu","doi":"10.1039/D4TB02090A","DOIUrl":"10.1039/D4TB02090A","url":null,"abstract":"<p >Electrocorticography (ECoG) has garnered widespread attention owing to its superior signal resolution compared to conventional electroencephalogram (EEG). While ECoG signal acquisition entails invasiveness, the invasive rigid electrode used inevitably inflicts damage on brain tissue. Polymer electrodes that combine conductivity and transparency have garnered great interest because they not only facilitate high-quality signal acquisition but also provide additional insights while preserving the health of the brain, positioning them as the future frontier in the brain–computer interface (BCI). This review summarizes the multifaceted functions of polymers in ECoG thin-film electrodes for the BCI. We present the abilities of sensitive and structural polymers focusing on impedance reduction, signal quality improvement, good flexibility, and transparency. Typically, two sensitive polymers and four structural polymers are analyzed in detail in terms of ECoG electrode properties. Moreover, the underlying mechanism of polymer-based electrodes in signal quality enhancement is revealed. Finally, the remaining challenges and perspectives are discussed.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 2","pages":" 454-471"},"PeriodicalIF":6.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d4tb02090a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NIR emissive probe for fluorescence turn-on based dead cell sorting and in vivo viscosity mapping in C. elegans†","authors":"Goraksha T. Sapkal, Farhan Anjum, Abdul Salam, Bodhidipra Mukherjee, Shilpa Chandra, Purabi Bala, Richa Garg, Shagun Sharma, Kush Kaushik and Chayan Kanti Nandi","doi":"10.1039/D4TB01945H","DOIUrl":"10.1039/D4TB01945H","url":null,"abstract":"<p >Dead cell sorting is pivotal and plays a very significant role in homeostasis. Apoptosis and ferroptosis are the two major regulatory cell death processes. Apoptosis is a programmed cell death process, while ferroptosis is a regulatory cell death process. Monitoring the dead cells coming out from these processes is extremely important to stop various cellular dysfunctions. Here, we present a single NIR emissive probe that can observe both apoptotic and ferroptosis regulatory cell deaths. We were able to directly visualize the dead cells in both animal and plant cells upon a significant increase in the fluorescence intensity of the probe. During cell death, the increased cytoplasm viscosity restricted the rotor motion and helped in the fluorescence turn-on of the probe. Lysosomal viscosity was found to play a crucial role in the ferroptosis pathway. On the other hand, the probe was not only efficient in mapping the viscosity in various parts of live <em>Caenorhabditis elegans</em> (<em>C. elegans</em>) bodies but also able to differentiate between live and dead animals.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 1","pages":" 184-194"},"PeriodicalIF":6.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142634953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinspired programmable coacervate droplets and self-assembled fibers through pH regulation of monomers†","authors":"Satyajit Patra, Sushmitha Chandrabhas and Subi J. George","doi":"10.1039/D4TB01550A","DOIUrl":"10.1039/D4TB01550A","url":null,"abstract":"<p >Phase separation and phase transitions pervade the biological domain, where proteins and RNA engage in liquid–liquid phase separation (LLPS), forming liquid-like membraneless organelles. The misregulation or dysfunction of these proteins culminates in the formation of solid aggregates <em>via</em> a liquid-to-solid transition, leading to pathogenic conditions. To decipher the underlying mechanisms, synthetic LLPS has been examined through complex coacervate formation from charged polymers. Nonetheless, temporal control over phase transitions from prebiotically relevant small organic synthons remains largely unexplored. Herein, we propose utilizing pH modulation to regulate the charge of small molecular building blocks, thereby controlling the LLPS process. Through a bio-inspired, enzyme-mediated pH-regulated reaction, we introduce temporal control over both LLPS and the transition from coacervates to supramolecular polymers. Additionally, by incorporating antagonistic pH modulators, we achieve transient LLPS and further temporal regulation of supramolecular polymer disassembly. Our investigation into pH-regulated LLPS provides a new avenue for exploring the stimuli-responsive, dynamic, and transient nature of LLPS.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 2","pages":" 604-609"},"PeriodicalIF":6.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142712276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantum DFT analysis and molecular docking investigation of various potential breast cancer drugs","authors":"Md Ashraf Ayub, Ankit Raj Tyagi, Sunil Kumar Srivastava and Pranveer Singh","doi":"10.1039/D4TB01803F","DOIUrl":"10.1039/D4TB01803F","url":null,"abstract":"<p >Breast cancer is among the deadliest cancers worldwide, highlighting the urgent need for effective treatments. This study employs density functional theory (DFT) and molecular docking analyses to evaluate the anti-cancer efficacy and specificity of drug molecules lapatinib, tucatinib, neratinib, anastrozole, and letrozole. DFT analysis provides comprehensive insights into the structural, electronic, optical, and vibrational properties of these drugs, helping to elucidate their molecular stability and reactivity through global reactivity descriptors. Additionally, molecular docking simulations reveal the binding conformations and interaction profiles of these drugs with key breast cancer targets, underscoring their therapeutic potential. Docking results indicate that lapatinib, tucatinib, and neratinib have high binding affinities for HER2, with lapatinib exhibiting the strongest overall binding, particularly with PDK1 (PDB ID: 1UU7), PAK4 (PDB ID: 2X4Z), GSK3 (PDB ID: 1GNG), and HER2 (PDB ID: 2IOK). The stable hydrogen bonding and other interactions observed with lapatinib support its effectiveness in treating HER2-positive breast cancers, tucatinib's selective HER2 binding reduces off-target effects, while neratinib's irreversible binding provides prolonged inhibition, making it useful for overcoming resistance in HER2-positive cases. In contrast, anastrozole and letrozole show lower binding affinities for HER2 and EGFR due to their simpler structures but are potent aromatase inhibitors, making them effective in treating estrogen receptor-positive (ER-positive) breast cancers. In conclusion, DFT and molecular docking studies affirm the suitability of lapatinib, tucatinib, and neratinib for HER2-positive cancers, while anastrozole and letrozole are effective in ER-positive cancers, emphasizing the role of molecular structure and binding affinity in optimizing cancer treatment strategies.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 1","pages":" 218-238"},"PeriodicalIF":6.1,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142634959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-friendly self-cleaning coatings: fundamentals, fabrication, applications, and sustainability†","authors":"Tanaji K. Chavan and Sushanta K. Sethi","doi":"10.1039/D4TB01392A","DOIUrl":"10.1039/D4TB01392A","url":null,"abstract":"<p >Eco-friendly self-cleaning coatings have garnered significant attention due to their potential to address environmental concerns while offering remarkable properties. This review explores the dynamic field of such coatings, focusing on their fundamental principles, fabrication techniques, applications, and sustainability. The main findings of this review shed light on the fundamentals of a wetting phenomenon that underpins superhydrophobicity and self-cleaning, revealing how bio-inspired approaches and sustainable materials have enabled the development of sustainable coatings. This review is structured around the fundamental principles of superhydrophobicity, discussing the basic mechanisms and following different approaches to eco-friendly coatings, focusing on bio-inspired methods and sustainable materials. Next, detailed fabrication techniques are discussed to create such coatings followed by various applications across industries, emphasizing the real-world impact of eco-friendly coatings. The next section discusses the various advantages followed by investigating the environmental implications and discussing how these coatings contribute to sustainability. The review concludes with commercial superhydrophobic self-cleaning products, which reflect the current state of research, outlining the challenges, and providing insights into future directions and innovations in this field. By providing an in-depth analysis of their fabrication techniques, applications, and potential future directions, it serves as a valuable resource for researchers and engineers seeking to design eco-friendly superhydrophobic coatings.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 2","pages":" 429-453"},"PeriodicalIF":6.1,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142690107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a xanthene-based NIR fluorescent probe for accurate and sensitive detection of γ-glutamyl transpeptidase in cancer diagnosis and treatment†","authors":"Chia-Kai Lai, Kuppan Magesh, Sivan Velmathi and Shu-Pao Wu","doi":"10.1039/D4TB01841A","DOIUrl":"10.1039/D4TB01841A","url":null,"abstract":"<p >γ-Glutamyl transpeptidase (GGT) regulates glutathione (GSH), essential for cell functions and linked to cancer. High GGT levels in tumors make it a valuable cancer biomarker. Current GGT detection methods often lack sensitivity and specificity. To address this, we developed <strong>XM-Glu</strong>, a new near-infrared (NIR) fluorescent probe. <strong>XM-Glu</strong> features a xanthene-based structure with a hydroxy xanthene fluorophore and a malononitrile group for NIR emission and reduced background noise. It has a self-immolating linker masked with glutamate acid, which activates fluorescence when GGT is present. <strong>XM-Glu</strong> can detect GGT in the range of 1.0 to 20 mU with a low detection limit of 0.067 mU mL<small>⁻¹</small>. It showed high specificity and minimal interference in cellular assays. In mice, <strong>XM-Glu</strong> effectively detected GGT in tumor, liver, and kidney tissues. Its NIR properties provide real-time insights into GGT activity, improving cancer diagnosis and monitoring. This new technology enhances cancer research and helps better understand GGT's role in cancer progression.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 1","pages":" 201-206"},"PeriodicalIF":6.1,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface-enhanced Raman scattering for HSP 70A mRNA detection in live cells using silica nanoparticles and DNA-modified gold nanoparticles†","authors":"Ju Eun Cho and Dong-Kwon Lim","doi":"10.1039/D4TB01514B","DOIUrl":"10.1039/D4TB01514B","url":null,"abstract":"<p >Real-time monitoring of mRNA in living cells is crucial for understanding dynamic biological processes. Traditional methods such as northern blotting, PCR, and sequencing require cell lysis and do not allow for continuous observation. Fluorescence-based techniques have advanced this field, but they are limited by photobleaching, which hinders long-term monitoring. In this study, we designed a dual-probe system combining fluorescence and surface-enhanced Raman scattering (SERS) signals to monitor mRNA in living cells. Our system uses silica nanoparticles (SiNPs) with DNA sequences which are hybridized with fluorescent DNA sequences and DNA-modified gold nanoparticles (AuNPs) to detect heat shock protein 70A mRNA, which can be induced by photothermal damage from laser exposure. Following nanoparticle uptake and induction of heat shock, we observed a time-dependent decrease in fluorescence intensity and increase in SERS intensity, indicating successful mRNA monitoring in living cells. These findings suggest that our dual-probe system with SiNPs and AuNPs is a promising nanotechnological platform for sensitive, long-term monitoring of gene expression in living cells, offering significant potential for future biological and medical research.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 2","pages":" 562-567"},"PeriodicalIF":6.1,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}