Journal of Materials Chemistry B最新文献

{"title":"Small molecule interactions with biomacromolecules: selective sensing of human serum albumin by a hexanuclear manganese complex – photophysical and biological studies†","authors":"Rousunara Khatun, Malay Dolai, Mihir Sasmal, Atul Katarkar, Abu Saleh Musha Islam, Nasima Yasmin, Sana Maryum, Jebiti Haribabu and Mahammad Ali","doi":"10.1039/D4TB00712C","DOIUrl":"10.1039/D4TB00712C","url":null,"abstract":"<p >A covalently bonded hexanuclear neutral complex, [Mn<small>₆</small>(μ<small>₃</small>-O)<small>₂</small>(3-MeO-salox)<small>₆</small>(OAc)<small>₂</small>(H<small>₂</small>O)<small>₄</small>] (<strong>1</strong>), has been synthesized and characterized by single crystal X-ray diffraction analysis along with IR and HRMS studies. Complex <strong>1</strong> has been found to selectively interact with human serum albumin (HSA), a model transport protein. The interaction of <strong>1</strong> with HSA was investigated by monitoring the change in the absorbance value of HSA at <em>λ</em> = 280 nm with increasing concentration of <strong>1</strong>. Likewise, fluorescence titrations were carried out under two conditions: (i) titration of a 5 μM solution of complex <strong>1</strong> with the gradual addition of HSA, showing a ∼9-fold fluorescence intensity enhancement at 424 nm, upon excitation at 300 nm; and (ii) upon excitation at 295 nm, titration of 5 μM HSA solution with the incremental addition of complex <strong>1</strong>, showing a quenching of fluorescence intensity at 334 nm, with simultaneous development of a new emission band at 424 nm. A linear form of the Stern–Volmer equation gives <em>K</em><small>_SV</small> = 9.77 × 10<small>⁴</small> M<small>⁻¹</small> and the Benesi–Hildebrand plot yields the binding constant as <em>K</em><small>_BH</small> = 1.98 × 10<small>⁵</small> M<small>⁻¹</small> at 298 K. The thermodynamic parameters, Δ<em>S</em>°, Δ<em>H</em>°, and Δ<em>G</em>°, were estimated by using the van’t Hoff relationship which infer the major contribution of hydrophobic interactions between HSA and <strong>1</strong>. It was observed that quenching of HSA emission arises mainly through a dynamic quenching mechanism as indicated by the dependence of average lifetime 〈<em>τ</em>〉 on the concentration of <strong>1</strong>. The changes in the CD (circular dichroism) spectral pattern of HSA in the presence of <strong>1</strong> clearly establish the variation of HSA secondary structure on interaction with <strong>1</strong>. The most probable interaction region in HSA for <strong>1</strong> was determined from molecular docking studies which establish the preferential trapping of <strong>1</strong> in the subdomain IIA of site I in HSA and substantiated by the results of site-specific marker studies. Complex <strong>1</strong> was further evaluated for its antiproliferative effects in lung cancer A549 cells, which strictly inhibits the growth of the cells in both 2D and 3D mammospheres, indicating its potential application as an anticancer drug.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142083004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A chondroitin sulphate hydrogel with sustained release of SDF-1α for extensive cartilage defect repair through induction of cell homing and promotion of chondrogenesis†","authors":"Yuezhou Wu, Zhuocheng Lyu, Fei Hu, Linjun Yang, Ke Yang, Mo Chen and You Wang","doi":"10.1039/D4TB00624K","DOIUrl":"10.1039/D4TB00624K","url":null,"abstract":"<p >Articular cartilage damage represents a prevalent clinical disease in orthopedics, with its regeneration and repair constituting a central focus in ongoing research endeavors. While hydrogel technology has achieved notable progress in the field of cartilage regeneration, addressing the repair of larger cartilage defects remains a significant and formidable challenge. In pursuit of achieving the repair of extensive cartilage defects, this study designed a polydopamine-modified chondroitin sulfate hydrogel loaded with SDF-1α (P-SCMA). This hydrogel, capable of directly providing glycosaminoglycans (GAGs), served as a platform for carrying growth factors and attracting mesenchymal stem cells for the <em>in situ</em> reconstruction of extensive cartilage defects. The results indicate that the P-SCMA hydrogel is capable of not only directly providing GAGs but also sustainably releasing SDF-1α. In the early stages, it promotes cell adhesion and proliferation and induces cell homing, while in the later stages, it further induces chondrogenesis by inhibiting the Wnt/β-catenin pathway. This bioactive hydrogel, which possesses the functions of providing GAGs, promoting cell proliferation, inducing cell homing and chondrogenesis, is capable of promoting cartilage repair in multiple ways, providing new perspectives for the repair of extensive cartilage defects.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141904019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colloidal photonic crystals towards biological applications","authors":"Zixin Shu, Xiaoning Sun, Xinyuan Xu, Meng Qin and Jianshu Li","doi":"10.1039/D4TB01325E","DOIUrl":"10.1039/D4TB01325E","url":null,"abstract":"<p >Colloidal photonic crystals (CPCs), fabricated from the assembly of micro-/nano-particles, have attracted considerable interest due to their unique properties, such as structural color, slow-photon effect, and high specific surface area (SSA). Benefiting from these properties, significant progress has been made in the biological applications of CPCs. In this perspective, these properties and relative manipulation strategies are firstly discussed, building bridges between properties and biological applications of CPCs. Structural color endows CPCs with naked-eye sensing capability, which can be applied to physiological state assessment and diagnosis, as well as self-report of CPC-based diagnostic and therapeutic devices. The slow-photon effect contributes to enhanced fluorescence, surface-enhanced Raman scattering, and efficacy of photodynamic/photothermal therapy, when CPCs are combined with corresponding functional materials. High SSA provides CPCs with abundant binding sites and superior capabilities for loading, adsorption, delivery, <em>etc.</em> These properties can be utilized individually or synergistically to grant CPCs superior performance in biological applications. Next, the recent advancements of CPCs towards biological applications are summarized, including biosensors, wound dressings, cells-on-a-chip, and phototherapy. Finally, a perspective on the challenges and future development of CPCs for biological applications is presented.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142006198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactivity and in vitro immunological studies of xenogeneic decellularized extracellular matrix scaffolds for implantable applications","authors":"Qing Yu, Yuantao Gao, Jiqiang Guo, Xinyue Wang, Xiang Gao, Yifan Zhao, Yang Liu, Meiling Wen, Xiangyu Zhang and Meiwen An","doi":"10.1039/D4TB00450G","DOIUrl":"10.1039/D4TB00450G","url":null,"abstract":"<p >Decellularized scaffolds retain the main bioactive substances of the extracellular matrix, which can better promote cell proliferation and matrix reconstruction at the defect site, and have great potential for morphological and functional restoration in patients with tissue defects. Due to the safety of the material source of allogeneic decellularized scaffolds, there is a great limitation in their clinical application, so the preparation and evaluation of xenodermal acellular scaffolds have attracted much attention. In terms of skin tissue structure and function, porcine skin has a high degree of similarity to human skin and has the advantages of sufficient quantity and no ethical issues. However, there is a risk of immune rejection after xenodermal acellular scaffold transplantation. To address the above problems, this paper focuses on porcine dermal decellularized scaffolds prepared using two common decellularization preparation methods and compares the decellularization efficiency, retention of active components of the extracellular matrix, structural characterization of the decellularized scaffolds, and the effect of porcine dermal decellularized scaffolds on mouse Raw264.7 macrophages, so as to make a functional evaluation of the active components and immune effects of porcine dermal decellularized scaffolds, and to provide a reference for filling trauma-induced defects in humans.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142074861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and characterization of macrophage membrane camouflaged cubosomes as a stabilized and immune evasive biomimetic nano-DDS†","authors":"Xuehui Rui, Yukihiro Okamoto, Nozomi Morishita Watanabe, Taro Shimizu, Ward Wakileh, Naoko Kajimura and Hiroshi Umakoshi","doi":"10.1039/D4TB01063A","DOIUrl":"10.1039/D4TB01063A","url":null,"abstract":"<p >This study aims to develop a biomimetic nano-drug delivery system (nano-DDS) by employing a macrophage cell membrane camouflaging strategy to modify lyotropic liquid crystal nanoparticles (LLC-NPs). The cubic-structured LLC-NPs (Cubosomes, CBs) were prepared <em>via</em> a top-down approach (ultra-sonification) using monoolein (MO) and doped with the cationic lipid, DOTAP. The cell membrane camouflaging procedure induced changes in the cubic lipid phase from primitive cubic phase (Q<small>_II</small><small>^P</small>) to a coexistence of Q<small>_II</small><small>^P</small> and diamond cubic phase (Q<small>_II</small><small>^D</small>). The macrophage membrane camouflaging strategy protected CB cores from the destabilization by blood plasma and enhanced the stability of CBs. The <em>in vitro</em> experiment results revealed that the macrophage cell membrane coating significantly reduced macrophage uptake efficacy within 8 h of incubation compared to the non-camouflaged CBs, while it had minimal impact on cancer cell uptake efficacy. The macrophage membrane coated CBs showed lower accumulation in the heart, kidney and lungs in vivo. This study demonstrated the feasibility of employing cell membrane camouflaging on CBs and confirmed that the bio-functionalities of the CBs-based biomimetic nano-DDS were retained from the membrane source cells, and opened up promising possibilities for developing an efficient and safe drug delivery system based on the biomimetic approach.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141918406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenium nanoparticle-functionalized injectable chitosan/collagen hydrogels as a novel therapeutic strategy to enhance stem cell osteoblastic differentiation for bone regeneration","authors":"Khaled Alajmi, Matthew Hartford, Nakka Sharmila Roy, Anamitra Bhattacharya, Santanu Kaity, Brenton L. Cavanagh, Subhadeep Roy and Kulwinder Kaur","doi":"10.1039/D4TB00984C","DOIUrl":"10.1039/D4TB00984C","url":null,"abstract":"<p >Stem cells are an essential consideration in the fields of tissue engineering and regenerative medicine. Understanding how nanoengineered biomaterials and mesenchymal stem cells (MSCs) interact is crucial for their role in bone regeneration. Taking advantage of the structural stability of selenium nanoparticles (Se-NPs) and biological properties of natural polymers, Se-NPs-functionalized, injectable, thermoresponsive hydrogels with an interconnected molecular structure were prepared to identify their role in the osteogenic differentiation of different types of mesenchymal stem cells. Further, comprehensive characterization of their structural and biological properties was performed. The results showed that the hydrogels undergo a sol to gel transition with the help of β-glycerophosphate, while functionalization with Se-NPs significantly enhances their biological response through stabilizing their polymeric structure by forming Se–O covalent bonds. Further results suggest that Se-NPs enhance the differentiation of MSCs toward osteogenic lineage in both the 2D as well as 3D. We demonstrated that the Se-NPs-functionalized hydrogels could enhance the differentiation of osteoporotic bone-derived MSCs. We also focused on specific cell surface marker expression (CD105, CD90, CD73, CD45, CD34) based on the exposure of healthy rats’ bone marrow-derived stem cells (BMSCs) to the Se-NP-functionalized hydrogels. This study provides essential evidence for pre-clinical/clinical applications, highlighting the potential of the nanoengineered biocompatible elastic hydrogels for bone regeneration in diseased bone.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A fluorescent “Turn-ON” probe with rapid and differential response to HSA and BSA: quantitative detection of HSA in urine†","authors":"Rohini Gupta and Kamaldeep Paul","doi":"10.1039/D4TB00749B","DOIUrl":"10.1039/D4TB00749B","url":null,"abstract":"<p >The present study provides insight into the differential response of a benzimidazole-malononitrile fluorescent “Turn-ON” probe on interaction with two structurally similar proteins, BSA and HSA. Compound <strong>6</strong> shows more sensitivity towards the two SAs, which is completely lost in the case of compound <strong>7</strong>, synthesized by substitution on <strong>6</strong>. The aggregates of compound <strong>6</strong> show absorption maxima at 385 nm and weak emission maxima at 565 nm. Compound <strong>6</strong> forms a new emission band at 475 nm on gradual addition of BSA (200 μM) along with a slight increase in the emission band at 565 nm. However, on addition of HSA (50 μM), a new band at 475 nm is formed. In contrast to BSA, in the case of HSA, 50% quenching is observed in the emission band of compound <strong>6</strong> at 565 nm. The new band formed on the interaction of <strong>6</strong> with BSA shows four-fold more enhancement compared to HSA. Furthermore, the mechanism of interaction of <strong>6</strong> with serum albumin has been investigated through lifetime-fluorescence analysis, site-selective drug experiments, dynamic light scattering, FE-SEM, FT-IR, <em>etc.</em> Molecular docking studies and site marker drug displacement experiments reveal differential interactions of <strong>6</strong> towards the two structurally similar proteins. Aggregates of <strong>6</strong> with an average hydrodynamic size of 100–190 nm are disassembled on adding BSA and HSA, and the size of the serum albumin and <strong>6</strong> complex decreases to 10–20 nm, revealing the ligand's encapsulation in the serum albumin cavity. Practical applicability for the quantitative detection of HSA in human urine samples is also demonstrated. The high binding affinity, sensitivity, selectivity and differential response of probe <strong>6</strong> towards two serum albumins (HSA and BSA) and significant quantification of HSA in urine samples shows the potential ability of this probe in medical applications.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/tb/d4tb00749b?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142001547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemo-photothermal therapy of bacterial infections using metal–organic framework-integrated polymeric network coatings†","authors":"Lulu Yu, Huajun Wu, Gnanasekar Sathishkumar, Xiaodong He, Runlong Ran, Kai Zhang, Xi Rao, En-Tang Kang and Liqun Xu","doi":"10.1039/D4TB00237G","DOIUrl":"10.1039/D4TB00237G","url":null,"abstract":"<p >Surface modification of biomedical materials and devices using versatile nanocomposite coatings holds great promise for improving functionalities to defend against life-threatening bacterial infections. In this study, a one-step surface modification strategy was developed to deposit gold nanorods (AuNRs)- and curcumin (CUR)-encapsulated zeolitic imidazolate framework-8 (ZIF-8) nanoparticles (AuNRs-ZIF-CUR NPs or AZC) onto phytic acid (PA)-ε-polylysine (Ply) network coatings. In the solution mixture of PA, Ply and AZC, PA interacted with Ply <em>via</em> electrostatic interactions, and can also bind to AZC <em>via</em> metal chelation. The as-formed AZC–PA–Ply aggregates could be deposited onto various substrates <em>via</em> surface adhesion of PA and gravitational effects. The physicochemical and antibacterial properties of the AZC–PA–Ply network coatings on polydimethylsiloxane (PDMS) substrates were evaluated. The sustained release of zinc ions and CUR, as well as the contact-killing ability of Ply, endowed the AZC–PA–Ply network coatings with good antibacterial chemotherapeutic effects. In addition, the embedded AuNRs in the AZC–PA–Ply network coatings exhibited excellent photothermal conversion efficiency for the ablation of bacteria. Upon near-infrared (NIR) laser irradiation, the AZC–PA–Ply-coated PDMS surfaces exhibited strong antibacterial effects by disrupting the membrane integrity and cellular functions of the adhered bacteria. Thus, the AZC-PA-Ply network coatings displayed combined antibacterial chemotherapeutic and photothermal therapeutic effects. Furthermore, the AZC–PA–Ply-coated PDMS substrates exhibited effective bacterial infection prevention and good biocompatibility in an <em>in vivo</em> implant model. Hence, the versatile AZC–PA–Ply network coatings are potentially useful as a multi-modal antibacterial platform to eliminate infectious bacterial pathogens in biomedical applications.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel method for the diagnosis of atherosclerosis based on nanotechnology","authors":"Ying Yang, Jiangpeng Pan, Aifeng Wang, Yongcheng Ma, Ying Liu and Wei Jiang","doi":"10.1039/D4TB00900B","DOIUrl":"10.1039/D4TB00900B","url":null,"abstract":"<p >Cardiovascular disease (CVD) is a global health concern, presenting significant risks to human health. Atherosclerosis is among the most prevalent CVD, impacting the medium and large arteries in the kidneys, brain, heart, and other vital organs, as well as the lower limbs. As the disease progresses, arterial obstruction can result in heart attacks and strokes. Therefore, patients with atherosclerosis should receive accurate diagnosis and timely therapeutic intervention. With the advancements in nanomedicine, researchers have proposed new research strategies and methods for atherosclerosis imaging. This paper summarizes some current research findings on the use of nanomaterials in diagnosing atherosclerosis and offers insights for optimizing the imaging applications of nanomaterials in the future.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142038117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decellularized extracellular matrix-based bioengineered 3D breast cancer scaffolds for personalized therapy and drug screening","authors":"Teeshyo Bhattacharya, Mamta Kumari, Kulwinder Kaur, Santanu Kaity, Somasundaram Arumugam, Velayutham Ravichandiran and Subhadeep Roy","doi":"10.1039/D4TB00680A","DOIUrl":"10.1039/D4TB00680A","url":null,"abstract":"<p >Breast cancer (BC) is the second deadliest cancer after lung cancer. Similar to all cancers, it is also driven by a 3D microenvironment. The extracellular matrix (ECM) is an essential component of the 3D tumor micro-environment, wherein it functions as a scaffold for cells and provides metabolic support. BC is characterized by alterations in the ECM. Various studies have attempted to mimic BC-specific ECMs using artificial materials, such as Matrigel. Nevertheless, research has proven that naturally derived decellularized extracellular matrices (dECMs) are superior in providing the essential <em>in vivo</em>-like cues needed to mimic a cancer-like environment. Developing <em>in vitro</em> 3-D BC models is not straightforward and requires extensive analysis of the data established by researchers. For the benefit of researchers, in this review, we have tried to highlight all developmental studies that have been conducted by various scientists so far. The analysis of the conclusions drawn from these studies is also discussed. The advantages and drawbacks of the decellularization methods employed for generating BC scaffolds will be covered, and the review will shed light on how dECM scaffolds help develop a BC environment. The later stages of the article will also focus on immunogenicity issues arising from decellularization and the origin of the tissue. Finally, this review will also discuss the biofabrication of matrices, which is the core part of the bioengineering process.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142006110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}