Journal of Materials Chemistry B最新文献

{"title":"Design and characterization of β-tricalcium phosphate-based self-passivating coatings on magnesium alloys†","authors":"Erdem Şahin, Roberta Ruggiero, Marco Tatullo, Francesco Paduano, Meltem Alp and Ahmed Şeref","doi":"10.1039/D4TB01214C","DOIUrl":"10.1039/D4TB01214C","url":null,"abstract":"<p > <em>Background</em>: Magnesium alloys degrade rapidly in salt solutions, which limits their use without passivating treatments. AZ31 alloy is particularly promising for implant applications owing to its biodegradability and mechanical properties, necessitating effective corrosion-resistant coatings. <em>Aim</em>: In this study, a self-passivating reactive coating was designed and evaluated for AZ31 magnesium alloy plates using β-tricalcium phosphate (TCP) to enhance corrosion resistance and biocompatibility. <em>Methods</em>: Solutions of TCP, trisodium citrate, magnesium nitrate, hydroxyethyl cellulose (HEC), and sodium chloride were used to dip-coat AZ31 plates. The coated samples were immersed in 3.5 wt% NaCl solution. Phase evolution was analysed using gravimetry, X-ray diffraction (XRD), energy-dispersive X-ray (EDX) spectroscopy, and scanning electron microscopy (SEM). The biological response of the coated samples was evaluated through MTT and resazurin assays. <em>Results</em>: The coating formed a stable TCP/HEC layer that gradually dissolved over two weeks, converting the surface to magnesium hydroxide, magnesium oxychloride, and magnesium phosphate phases. The formation of brucite, responsible for passivation in the long term, was observed. The coating effectively prevented excessive magnesium oxychloride formation and stabilised magnesium hydroxide after one week. Biological characterization indicated that the coating on AZ31 is safe on the Saos-2 and L929 cell lines. <em>Conclusion</em>: The TCP-based coating enhances the corrosion resistance of AZ31 alloy in salt solutions, promoting passivating phases and limiting corrosive products, thereby ameliorating biocompatibility issues. This coating demonstrates substantial potential for extending the longevity and functionality of magnesium alloy implants.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 44","pages":" 11477-11490"},"PeriodicalIF":6.1,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Development and characterization of a novel poly(N-isopropylacrylamide)-based thermoresponsive photoink and its applications in DLP bioprinting","authors":"Kalindu D. C. Perera, Sophia M. Boiani, Alexandra K. Vasta, Katherine J. Messenger, Sabrina Delva and Jyothi U. Menon","doi":"10.1039/D4TB90152E","DOIUrl":"10.1039/D4TB90152E","url":null,"abstract":"<p >Correction for ‘Development and characterization of a novel poly(<em>N</em>-isopropylacrylamide)-based thermoresponsive photoink and its applications in DLP bioprinting’ by Kalindu D. C. Perera <em>et al.</em>, <em>J. Mater. Chem. B</em>, 2024, https://doi.org/10.1039/D4TB00682H.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 38","pages":" 9780-9780"},"PeriodicalIF":6.1,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11420647/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142309500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzymatic self-assembly of short peptides for cell spheroid formation†‡","authors":"Jiaqi Guo, Weiyi Tan and Bing Xu","doi":"10.1039/D4TB01154F","DOIUrl":"10.1039/D4TB01154F","url":null,"abstract":"<p >Cell spheroids, including organoids, serve as a valuable link between <em>in vitro</em> systems and <em>in vivo</em> animal models, offering powerful tools for studying cell biology in a three-dimensional environment. However, existing methods for generating cell spheroids are time consuming or difficult to scale up for large-scale production. Our recent study has revealed that transcytotic peptide assemblies, which transform from nanoparticles to nanofibers by enzymatic reactions, can create an intercellular fibril/gel, accelerating cell spheroid formation from a 2D cell culture or a cell suspension. While this finding presents an alternative approach for generating cell spheroids, the specific structural features required for efficient cell spheroid formation remain unclear. Based on our observation that a phosphotetrapeptide with a biphenyl cap at its N-terminus enables cell spheroid formation, we produced 10 variants of the original peptide. The variants explored modifications to the peptide backbone, length, electronic properties of the biphenyl capping group, and the type of phosphorylated amino acid residue. We then evaluated their ability for inducing cell spheroid formation. Our analysis revealed that, among the tested molecules, peptides with C-terminal phosphotyrosine, low critical micelle concentration, and dephosphorylation-guided nanoparticle to nanofiber morphological transition were the most effective in inducing the formation of cell spheroids. This work represents the first example to correlate the thermodynamic properties (<em>e.g.</em>, self-assembling ability) and kinetic behavior (<em>e.g.</em>, enzymatic dephosphorylation) of peptides with the efficacy of controlling intercellular interaction, thus offering valuable insights into using enzymatic self-assembly to generate peptide assemblies for biological applications.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 43","pages":" 11210-11217"},"PeriodicalIF":6.1,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11540748/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142383013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel high signal-to-noise ratio fluorescent probe for real-time mitochondrial viscosity detection and imaging in vitro and in vivo†","authors":"Jinyu Fu, Simeng He, Jiandong Liu, Jiaojiao Pang, Kang-Nan Wang and Yuguo Chen","doi":"10.1039/D4TB01486C","DOIUrl":"10.1039/D4TB01486C","url":null,"abstract":"<p >Mitochondrial viscosity serves as a critical indicator for assessing mitochondrial functionality and offers valuable insights into cellular homeostasis. Continuous, real-time monitoring of mitochondrial viscosity is indispensable for understanding and diagnosing diseases associated with these dynamic changes. In this study, we introduce a novel mitochondrial viscosity-responsive probe named “<strong>JL-JC</strong>” which is designed by using a molecular strategy, with a classic “D–π–A” molecular structure. Leveraging the distinctive twisted intramolecular charge transfer (TICT) properties of the probe, <strong>JL-JC</strong> exhibits exceptional sensitivity and a high signal-to-noise ratio, enabling precise detection of viscosity variations within its microenvironment while remaining unaffected by other factors. Upon rapid cellular uptake, <strong>JL-JC</strong> can efficiently evaluate the mitochondrial viscosity changes under diverse physiological and pathological conditions. Notably, this probe also enables viscosity imaging in zebrafish, offering insights into mitochondrial states <em>in vivo</em>. Our findings present <strong>JL-JC</strong> as a promising tool and potential diagnostic platform for mitochondria-related diseases.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 41","pages":" 10635-10643"},"PeriodicalIF":6.1,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142304977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitive detection of dipeptidyl peptidase based on DNA–peptide conjugates and double signal amplification of CHA and DNAzymes†","authors":"Yan Chen, Miao He, Feifan Yin, Wenting Cheng, Zhongyun Wang and Yang Xiang","doi":"10.1039/D4TB01288G","DOIUrl":"10.1039/D4TB01288G","url":null,"abstract":"<p >Dipeptidyl peptidase IV (DPPIV) is an enzyme belonging to the type II transmembrane serine protease family that has gained wide interest in the fields of hematology, immunology, and cancer biology. Moreover, DPPIV has emerged as a promising target for therapeutic intervention in type II diabetes. Due to its biological limitations, traditional strategies cannot meet the requirements of low abundance DPPIV analysis in complex environments. In this work, combining the high programmability of DNA and the chemical diversity of peptides, we designed DNA–peptide conjugates that can be specifically recognized, polypeptides as specific substrates for target DPPIV and DNA probes as primers for catalytic hairpin assembly (CHA), recycling a large amount of DNAzymes by triggering CHA amplification. The DNAzyme substrate modified with the FAM fluorescent group was immobilized on the surface of gold nanoparticles by S–Au chemical bonds to form a signal output probe. The DNAzymes enzyme cleaved the substrate of the signal outputs probe, yielding a double-amplified fluorescence signal. This method has a detection limit as low as 0.18 mU mL<small>⁻¹</small> and a linear range of 0–5 mU mL<small>⁻¹</small> in serum samples, showing high stability and good potential for practical applications.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 41","pages":" 10656-10664"},"PeriodicalIF":6.1,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142304982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-strength fibrous sensors with an enhanced aggregate state for biomechanical monitoring of the Achilles tendon†","authors":"Ying Guo, Ting Yan, Han Gao, Luyi Sun, Shuanglei Wei, Jun Chen, Yanhong Wei, Guoyin Chen, Kai Hou and Meifang Zhu","doi":"10.1039/D4TB01255K","DOIUrl":"10.1039/D4TB01255K","url":null,"abstract":"<p >Continuous monitoring of biomechanical signals generated from the injured Achilles tendon is essential for the deep understanding of the recovery or rehabilitation process, thus decreasing the risk of secondary injuries. With tissue-like components and adjustable properties, hydrogel-based biomechanical sensors are considered promising materials for human motion detection. However, existing hydrogels are characterized by inferior mechanical properties with strength and modulus typically lower than 1 MPa, as well as poor stability under physiological conditions, which hampers their applications in implantable devices. Moreover, acquiring the stress signal from collected electrical signals remains challenging. Herein, based on the regulation of polymer aggregation, a high-strength fibrous sensor composed of polyvinyl alcohol (PVA) and reduced graphene oxide (rGO) for <em>in vivo</em> monitoring is prepared through a two-step procedure, including freeze–thaw and freeze–soak. Benefiting from the synergy of crystallization, Hofmeister effect and nanocomposite, the hydrogel fibers feature high tensile strength (8.34 ± 0.66 MPa) and elastic modulus (1.15 ± 0.10 MPa). Meanwhile, the removal of salt ions during fabrication improves the water content (69.18 ± 1.47%) and anti-swelling performance of such fibers and minimizes side effects after implantation. It is demonstrated that the fibrous sensor could record the relative resistance changes upon stretching with ideal sensitivity (GF = 1.57) and convert them into bearing stress through formula derivation and calculations. <em>In vitro</em> and <em>in vivo</em> assays further confirm its feasibility for real-time monitoring of joint motion, providing important references for medical diagnosis and treatment.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 41","pages":" 10605-10615"},"PeriodicalIF":6.1,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142304980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Piezoelectric materials for anti-infective bioapplications","authors":"Chen Chen, Xin Yang, Yi Liu, Jia Jia, Yiping Li, Xiaohan Dai and Ousheng Liu","doi":"10.1039/D4TB01589D","DOIUrl":"10.1039/D4TB01589D","url":null,"abstract":"<p >Bacterial infection severely limits the effectiveness of biomaterials for tissue repair, posing a major challenge to modern medicine. Despite advances in novel antibiotics and their application in treatment, challenges remain in clinical practice. To address this issue, biomaterials are engineered to achieve desirable anti-infective performance and compatibility <em>via</em> adjusting their surface physicochemical properties. Recently, numerous studies on piezoelectric materials have been performed for anti-infective and regenerative therapies, but a comprehensive review is still lacking. This article provides a brief overview of the different types of piezoelectric materials and their characteristics. Building on this understanding, this review highlights the antibacterial mechanisms including orchestrating electric field and optimizing piezoelectric catalysis, which promote infective tissue regeneration, as well as discusses the anti-infective bioapplication of piezoelectric materials. Furthermore, this review concludes with perspectives into the challenges and future research directions of piezoelectric biomaterials.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 43","pages":" 11063-11075"},"PeriodicalIF":6.1,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142396374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of quaternary ammonium based acrylic copolymer antimicrobial coatings for polyurethane tracheoesophageal voice prostheses†","authors":"Manjeet Singh, Aiman Afreen, Mohd Anees, Dinesh Kalyanasundaram, Harpal Singh and Naresh Bhatnagar","doi":"10.1039/D4TB01071J","DOIUrl":"10.1039/D4TB01071J","url":null,"abstract":"<p >Tracheoesophageal voice prostheses (TEPs) are soft polymeric devices used to restore the voices of patients who have undergone total laryngectomy (TL). TEPs are implanted on the tracheoesophageal wall and come in direct contact with food, fluid and air. The environment of an implant is a budding ground for growth of microbes, leading to biofilm formation on the TEP surfaces. Biofilm formation is the leading cause of TEP failure because the biofilm affects its functioning by clogging the air passage over several weeks to a few months. Short useful lifespan of TEPs is a concern for patients undergoing total laryngectomy. To increase the useful lifespan of TEPs, it is imperative to prevent biofilm formation. This problem is addressed in the present study by developing a cationic crosslinked antimicrobial coating that prevents biofilm formation through contact killing. An acrylate-based tetracopolymer poly(methyl methacrylate [MMA]-<em>co-n</em>butyl acrylate [BA]-<em>co</em>-dimethylaminoethyl methacrylate [DMAEMA]-<em>co</em>-2 hydroxyethyl methacrylate [HEMA]) was synthesized by free radical polymerization and was crosslinked by hexamethylene diisocyanate (THDI) trimer through urethane linkages. The crosslinked tetracopolymer coatings were subjected to quaternary ammonium formation through <em>N</em>-alkylation with iodooctane. Different variants of coatings were synthesized and the tetracopolymer with 22.5% MMA, 22.5% BA, 20% HEMA and 35% DMAEMA showed desirable results. The developed coatings were characterized and tested for safety as well as mechanical and antimicrobial efficacy. The final results showed that the developed coatings exhibited good cytocompatibility, haemocompatibility, mechanical properties and antimicrobial properties for 180 days against <em>E. coli</em>, <em>S. aureus</em> and <em>C. albicans.</em></p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 41","pages":" 10644-10655"},"PeriodicalIF":6.1,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142304979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural isomerism engineering regulates molecular AIE behavior and application in visualizing endogenous hydrogen sulfide†","authors":"Yaxi Li, Yong-Xiang Wang, Dujuan Liu, Chen-Chieh Ni, Jianming Ni and Jen-Shyang Ni","doi":"10.1039/D4TB01617C","DOIUrl":"10.1039/D4TB01617C","url":null,"abstract":"<p >Hydrogen sulfide (H<small>₂</small>S) is a critical bioregulator implicated in numerous physiological and pathological processes, including cancer and neurodegenerative diseases. Compared with traditional instrument analysis, fluorescence detection technology based on small molecules in real-time and <em>in situ</em> sensing H<small>₂</small>S has attracted attention. In this investigation, we developed a system of coumarin-based fluorophores linked with aminopyridine <em>via</em> a dipolar imino-double bond. Their aggregation-induced emission (AIE) behaviors were further regulated <em>via</em> structural isomerism engineering. Owing to restricting intramolecular motions and high molecular dipole moment, 2-amino-pyridyl-substituted coumarin (CMR-<em>o</em>-Py) forms stable AIE nanoaggregates with brighter fluorescence than the others. The CMR-<em>o</em>-Py nanoaggregates serve as probes for sensing H<small>₂</small>S with a detection limit of 18.1 μM in a hydrophilic environment <em>via</em> Michael addition between imino-bond and sulfide ions. The 1 : 1 stoichiometric binding energy constant between the probe and H<small>₂</small>S is 5.68 × 10<small>⁸</small> M<small>⁻¹</small>, and its half-time of the first-order binding reaction was estimated to be 4.85 min. Moreover, CMR-<em>o</em>-Py, with excellent biocompatibility, holds promise as an ideal sens<em>o</em>r for endogenous H<small>₂</small>S in living cells and onion tissues, further highlighting its potential application in biological fields.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 43","pages":" 11134-11141"},"PeriodicalIF":6.1,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142373941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DOTAGEL: a hydrogen and amide bonded, gelatin based, tunable, antibacterial, and high strength adhesive synthesized in an unoxidized environment†","authors":"Soham Irtiza Swapnil, Md Tashdid Hossain Shoudho, Abdur Rahman, Tahmed Ahmed and M. Tarik Arafat","doi":"10.1039/D4TB00608A","DOIUrl":"10.1039/D4TB00608A","url":null,"abstract":"<p >The development of bioadhesives that concurrently exhibit high adhesion strength, biocompatibility, and tunable properties and involve simple fabrication processes continues to be a significant challenge. In this study, a novel bioadhesive named DOTAGEL is synthesized by crosslinking gelatin (GA), dopamine (DA), and tannic acid (TA) in an unoxidized environment due to the advantage of controlling the degree of protonation in GA and TA, as well as controlling the degree of intermolecular amide and hydrogen bonding in the acidic medium. DOTAGEL (DA + TA + GA) shows superior adhesion strengths of 104.6 ± 46 kPa on dry skin and 35.6 ± 4.5 kPa on wet skin, up to 13 attachment–detachment cycles, retains adhesion strength under water for up to 10 days and is capable of joining two cut parts of internal organs of mice. Moreover, DOTAGEL shows strong antibacterial properties, self-healing, and biocompatibility since it contains TA, a natural and antibacterial cross-linker with abundant hydroxyl groups and the capability of forming non-covalent bonds in an unoxidized environment, and dopamine hydrochloride, a mussel inspired biomaterial containing both the amine and catechol groups for amide bonding and hydrogen bonding with TA and GA. The cross-linking among 20% (w/v) GA, 0.2% (w/v) DA, and 20% (w/v) TA is done by the centrifugation process at room temperature. Two different acids, hydrochloric acid and acetic acid, were used for tuning the pH of the medium, which led to two different samples named DOTAGEL/AA and DOTAGEL/HCL. The degree of cross-linking and mechanical and biochemical properties, like adhesion strength, degradation rate, antibacterial properties, stickiness, <em>etc.</em>, are tuned by adjusting the pH of the medium. DOTAGEL/HCL showed 6.5 times faster degradation in 10 days, a faster release rate in the antibacterial study, 2 times adhesion strength in a dry medium, and more stickiness. The novelty lies not only in increased adhesion strength but also in the single-step fabrication process of the adhesive in the acidic medium. This research proposes the formation of a tunable antibacterial adhesive that is capable of working on wet surfaces within the body and that has the potential to become a successful tissue adhesive with a wide range of possibilities in controlled drug delivery at wound sites and other biomedical applications.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 42","pages":" 11025-11041"},"PeriodicalIF":6.1,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142362605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}