Journal of Materials Chemistry B最新文献

{"title":"Bioorthogonal reaction mediated size transformation clustered nanosystems for potentiating bioimaging and drug delivery","authors":"Juan Li, Shan Yan, Jie Xu, Cao Li and Qi Yu","doi":"10.1039/D5TB01134E","DOIUrl":"10.1039/D5TB01134E","url":null,"abstract":"<p >Continuous advances have been witnessed in the booming of size variable nanosystems for molecular imaging and therapy. These nanosystems usually exhibit <em>in situ</em> size transformation, which promotes optimized biodistribution, retention and penetration in lesions. Bioorthogonal reactions have been introduced as a useful tool to develop size variable nanosystems. In general, researchers modify controllable block (including pH adjustment, disulfide reduction, and/or enzymatic hydrolysis) masked bioorthogonal handles on the nanoparticles or small molecules to develop biocompatible size variable nanosystems. These nanosystems undergo precise click cycloaddition and self-assemble into nanoaggregates <em>in situ</em>, showing enhanced tissue accumulation and retention. These advantages have demonstrated great promise in improving imaging quality and therapeutic outcomes with high effectiveness and controllability. To date, this strategy has been widely introduced to construct bioimaging probes or nanomedicines. To gain a comprehensive understanding of the strategy, in this review, we focus on bioorthogonal reaction mediated size variable nanosystems reported in the last five years, present their application in bioimaging and therapy, and elucidate the mechanism of bioorthogonal reactions. Based on these efforts, challenges and future research directions in this area are also discussed at the end.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 36","pages":" 11166-11200"},"PeriodicalIF":6.1,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances of Chinese herbal medicine-derived polysaccharides as carrier-free and carrier agents in anti-melanoma therapy","authors":"Yingmin Mao, Zhiqiang Dai, Qiuyan Zhang, Fuming Peng, Quan Li, Hanming Cui and Yue Liu","doi":"10.1039/D5TB01185J","DOIUrl":"10.1039/D5TB01185J","url":null,"abstract":"<p >Melanoma, a highly aggressive malignancy with increasing global incidence, faces significant therapeutic challenges due to drug resistance, systemic toxicity, and immunosuppressive tumor microenvironment. Despite advances in targeted therapies and immunotherapies, the need for safer and more effective treatment options remains urgent. Chinese herbal medicine-derived polysaccharides (CHMPs), characterized by their natural origin, biocompatibility, and multifaceted bioactivities, have garnered increasing attention as promising candidates for melanoma therapy due to their low toxicity and multi-targeting capabilities. In this review, the dual roles of CHMPs with varied origins in anti-melanoma treatment will be systematically elucidated. As carrier-free anti-tumor agents, CHMPs exert anti-melanoma effects through multiple mechanisms, including immune modulation, apoptosis induction, cell cycle arrest, inhibiting tumor angiogenesis, and regulating signaling pathways. Moreover, as intelligent carriers, CHMPs can be chemically modified or physically assembled into targeted delivery systems, enhancing drug stability and bioavailability while minimizing off-target toxicity. Finally, current challenges for CHMPs in anti-melanoma treatments, such as structural heterogeneity, unclear structure–activity relationships, and limited clinical translation, will be outlined, and future efforts are also discussed. By bridging traditional herbal medicine and modern biomaterials science, we expect this review not only to provide a roadmap for developing CHMP-based multifunctional biomaterials platforms but also to inspire novel strategies to overcome current therapeutic bottlenecks in melanoma management, paving the way for safer and more effective treatments.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 37","pages":" 11508-11524"},"PeriodicalIF":6.1,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional PAMAM nanoparticles with sequential antimicrobial-remineralization therapy for dentin caries management","authors":"Mingxiao Liu, Jiahe Li, Ziyou Wang, Miao Chen, Jianru Yi, Zhihe Zhao and Kunneng Liang","doi":"10.1039/D5TB01477H","DOIUrl":"10.1039/D5TB01477H","url":null,"abstract":"<p >Dentin caries is a multifactorial pathological process characterized by bacterial colonization and biofilm formation that result in concurrent acid-mediated demineralization and matrix metalloproteinase (MMP)-mediated degradation of the collagenous matrix. While remineralization therapies offer minimal invasiveness, their long-term efficacy is compromised by ongoing collagen degradation and persistent bacterial acid production that counteract remineralization efforts. To address these limitations, we designed PAMAM-G4@EG (PGE) nanoparticles (NPs) using polyamide amine (PAMAM) dendrimers as mineral deposition templates, with antimicrobial peptide G(IIKK)<small>₄</small>I–NH<small>₂</small> (G4) grafted onto the external surface groups and epigallocatechin gallate (EG) encapsulated within the internal cavities to provide biofilm disintegration and collagen protection for comprehensive dentin caries intervention. First, the PGE NPs reach lesion surfaces and accelerate EG release under acidic conditions. EG loosens <em>Streptococcus mutans</em> (<em>S. mutans</em>) biofilms, followed by G4-mediated disruption of planktonic <em>S. mutans</em> cell membranes. <em>In vitro</em> antimicrobial assays demonstrated a bactericidal efficacy of 99.75% after PGE intervention. Upon deeper lesion penetration, PGE releases EG to inhibit MMP activity and preserve the collagen scaffold, achieving a 74% reduction in hydroxyproline (HYP) levels. Simultaneously, PAMAM promotes controlled hydroxyapatite (HA) deposition, facilitating dentin remineralization. Treatment with PGE in artificial saliva containing collagenase restored dentin hardness to 89.88% of intact values. <em>In vivo</em> validation using a rat caries model confirmed therapeutic efficacy through significant reductions in Keyes scores, decreased salivary <em>S. mutans</em> counts, and increased molar mineral density. These findings demonstrate the therapeutic efficacy of PGE in dentin caries prevention and treatment, supporting its potential for clinical application.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 38","pages":" 12085-12100"},"PeriodicalIF":6.1,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in organic molecule fluorescent probes for microbial imaging","authors":"Kunyi Wang, Zixuan Zhang, Xuefan Guo, Guoyang Zhang, Yulong Jin and Zhuo Wang","doi":"10.1039/D5TB01373A","DOIUrl":"10.1039/D5TB01373A","url":null,"abstract":"<p >Microorganisms play a crucial role in human life and production. However, certain pathogenic microorganisms, such as bacteria, viruses, and fungi, can cause diseases like pneumonia, meningitis, and influenza, potentially leading to global public health crises. Fluorescence imaging has become a key tool for microbial detection due to its high specificity, sensitivity, and operational convenience. This review summarizes recent advances in microbial imaging studies based on organic small-molecule fluorescent probes. It focuses on the design strategies of various fluorophores, such as AIE, BODIPY, rhodamine, and fluorescein, and their application in imaging bacteria, fungi, and viruses. By targeting microbial-specific biomarkers, such as cell wall components, enzymes, or nucleic acid structures, these probes achieve high-selectivity imaging. These probes are applied in the detection of drug-resistant bacteria, biofilm research, and the development of anti-infective drugs. Additionally, the integration of multi-probe arrays with artificial intelligence opens new avenues for rapid microbial classification. This review discusses the principles of probe design, imaging mechanisms, and challenges, while also exploring their potential in clinical diagnostics and biological research.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 35","pages":" 10700-10714"},"PeriodicalIF":6.1,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144857222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in drug delivery systems based on liposome-composite hydrogel microspheres","authors":"Shuanghong Yang, Ze Li, Sicheng Li, Jinpeng Zhang, Jinjian Huang, Jianan Ren and Xiuwen Wu","doi":"10.1039/D5TB01369K","DOIUrl":"10.1039/D5TB01369K","url":null,"abstract":"<p >Research on liposome-composite hydrogel microspheres (LHMs) drug delivery systems, primarily composed of drugs, liposomes, and hydrogels, has garnered growing scientific interest. LHMs exhibit biosafety, modifiability, a wide range of loaded drug categories (water-soluble or fat-soluble), controlled and sustainable drug release capability, and specific cell-targeted performance, which compensate for the shortcomings of conventional drug delivery methods due to the complementary advantages of liposome and hydrogel microspheres. In this review, we systematically analyze the existing literature on LHMs and provide a comprehensive overview of their preparation methods. Specifically, we detail the fabrication techniques for liposomes, including thin-film hydration, solvent injection, multiple emulsion, reverse-phase evaporation, gradient, freeze-drying, supercritical fluid, and microfluidic approaches as well as methodologies for LHMs, such as microfluidics, electrospraying, 3D printing, reverse-phase microemulsion, and physical adsorption. We also summarize the optimization approaches for LHMs properties when combining liposomes and hydrogel microspheres. Finally, we present the applications and challenges of LHMs. We hope that this review will foster more insights into LHMs in drug delivery fields.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 38","pages":" 11885-11908"},"PeriodicalIF":6.1,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d5tb01369k?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144994928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Poly(N-acryloyl-l-phenylalanine) nanoparticles for potential treatment of inflammation in selective organs","authors":"Divya Pareek, Sukanya Patra, Md. Zeyaullah, Gurmeet Singh, Taniya Das, Prakriti S. Samanta, Aman S. Kudada, Anjali Mourya, Kirti Wasnik, Rajalaxmi Pradhan, Yitzhak Mastai and Pradip Paik","doi":"10.1039/D5TB00886G","DOIUrl":"10.1039/D5TB00886G","url":null,"abstract":"<p >Systemic inflammation can lead to multi-organ failure. The existing anti-inflammatory agents show adverse side effects, and the present situation demands new drugs with high therapeutic efficiency. Polymeric nanoparticles based on amino acids could be one of the best alternative solutions due to their cytocompatibility and immune responses. Herein, we synthesized polymeric nanoparticles (Phe NPs) with a size of 20–30 nm using <em>N</em>-acryloyl-<small>L</small>-phenylalanine methyl ester as a precursor. The biological and immune responses of Phe NPs were found to be commanding, which was proven using immune cells (RAW 264.7 macrophages). <em>In vitro</em> study revealed an easy uptake of these NPs (∼98%) by the immune cells and that they can reduce inflammation by improving the immune response. <em>In silico</em> molecular docking results revealed that Phe NPs could potentially interact with immune cytokines such as IL-6, NF-κβ, TNF-α, COX2 and IL-1β. Phe NPs exhibit a similar type of binding and interaction as ibuprofen (IBF), which confirms its immune response to control inflammation. The anti-inflammatory response of Phe NPs was established through an <em>in vitro</em> inflammation model developed using LPS-stimulated RAW 264.7 macrophages. Furthermore, an LPS-induced <em>in vivo</em> rat model was developed, which revealed that Phe NPs are useful for the treatment of systemic inflammation. Blood-based biochemical parameters such as C-reactive protein, lactate and procalcitonin levels were determined, and the anti-inflammatory responses of Phe NPs were confirmed through RT-PCR analysis by measuring the levels of inflammatory markers such as TNF-α, IL-6 and VEGF. Finally, an <em>in vivo</em> systemic inflammation rat model was used to examine the systemic organs (brain, liver, kidneys, spleen, lungs and heart) before and after treatment with Phe NPs to prove their anti-inflammatory responses. H&amp;E histological analysis of different organs further revealed that even at a low dose of 100 μg kg<small>⁻¹</small>, Phe NPs are immune-responsive/protective and anti-inflammatory in nature.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 37","pages":" 11767-11789"},"PeriodicalIF":6.1,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d5tb00886g?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A pH/ROS dual responsive smart microgel miRNA delivery system for repair of intervertebral disc degeneration","authors":"Weiqiang Lan, Chuan Guo, Yuheng Liu, Fei Ma, Weifei Zhang, Dengbo Yao, Yu Wang and Qingquan Kong","doi":"10.1039/D5TB01505G","DOIUrl":"10.1039/D5TB01505G","url":null,"abstract":"<p >The progression of intervertebral disc degeneration (IDD) is due to the progressive exacerbation of apoptosis and impaired extracellular matrix (ECM) synthesis, both of which are induced by progressive inflammation. Therefore, addressing the inflammatory microenvironment and correcting excessive apoptosis of nucleus pulposus cells (NPCs) are key to achieving intervertebral disc (IVD) regeneration. In this study, we designed a microenvironment-responsive smart microgel gene delivery system that for the first time combines phenylboronic acid-functionalized microgels with strontium sulfite nanoparticles to load miR-155 to enhance their anti-apoptosis capacity and promote ECM regenerative effects. In addition, strontium sulfite nanoparticles were able to respond to pH changes and dissolve at endosomal pH to release genetic materials. This highly biocompatible microgel drug system (MS-TSNPs@miR-155) was capable of continuously releasing miR-155, effectively modulating inflammation and attenuating apoptosis in NPCs. These minimally invasive and smart delivery capabilities promote the restoration of metabolic homeostasis within the medullary ECM, effectively delaying IDD progression.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 36","pages":" 11454-11469"},"PeriodicalIF":6.1,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insulin-producing INS-1 cell cultures on biomimetic 3D scaffolds","authors":"Mikhail Parker, Nataraja Sekhar Yadavalli, Kristina Peranidze, Eugene Boland, Vladimir Reukov and Sergiy Minko","doi":"10.1039/D5TB00519A","DOIUrl":"10.1039/D5TB00519A","url":null,"abstract":"<p >Three-dimensional cell cultures on biomimetic scaffolds have gained significant attention in tissue engineering, drug delivery, and scalable cell production. Current challenges in creating an ideal scaffold are providing maximum space for cells to grow while ensuring efficient nutrient, metabolite, and gas exchange to prevent the formation of necrotic or apoptotic regions. In our work, we grow insulin-producing INS-1 cells on touch-spun polycaprolactone (PCL) fiber scaffolds. Touch-spinning allows the creation of finely aligned 3D mesh-like fiber scaffolds with controllable distance between the fibers, resulting in a minimum of abiotic scaffold material and providing maximum space for cells to grow. Adding Matrigel at different combinations allowed us to control the INS-1 proliferation profile and grow them either in the form of scarce large (up to 1 mm) spheroids (no Matrigel), numerous smaller (about 150–200 μm in diameter) spheroids (Matrigel added to the cells only) or cell sheets (Matrigel added to both cells and fibers). Growing INS-1 cells as nanofiber-reinforced cell sheets is of utmost importance because it opens the possibility of using them in cell sheet tissue engineering. Obtaining free-floating sheets of insulin-producing cells by traditional means is typically challenging due to their fragility. Being only about 4–6 cells thick, INS-1 cell sheets are not prone to forming necrotic cores, which is a common problem for all 3D spheroid cultures when they reach a diameter of more than 150–200 μm. At the same time, they preserved their insulin production ability and characteristics of 3D cultures, such as numerous cell-to-cell contacts and metabolic activity.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 38","pages":" 12134-12145"},"PeriodicalIF":6.1,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d5tb00519a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction of high-performing spleen-targeted lipid nanoparticles using a deep learning model for robust anticancer immunotherapy","authors":"Honglei Zhang, Jingxuan Ma, Fulu Pan, Yangjian Liu, Man Zhang, Yuqing Li, Chao Zhang, Huajie Huang, Wannian Zhang, Donghui Xiu, Wei Zhang and Gengshen Song","doi":"10.1039/D5TB01217A","DOIUrl":"10.1039/D5TB01217A","url":null,"abstract":"<p >Messenger RNA (mRNA) therapeutics hold significant potential across a wide range of medical applications. LNPs are the most clinically advanced mRNA delivery vehicles, but challenges such as off-target effects and liver accumulation limit their broader clinical use. While high-throughput screening is effective for identifying more efficient and selective ionizable lipids, the substantial experimental validation required limits its practical application. In this study, we developed a deep learning model to accelerate ionizable lipid optimization by virtually predicting high-performing ionizable cationic lipids. After applying this model to a series of bis-hydroxyethylamine derived lipids (BDLs), 24 promising candidates were synthesized for delivery efficiency and organ-selectivity validation. Among them, YK-407 exhibited superior <em>in vitro</em> transfection efficiency and <em>in vivo</em> organ-specific mRNA delivery. YK-407 LNPs predominantly targeted the spleen, particularly antigen-presenting cells (APCs). In a mouse OVA tumor model, YK-407 LNPs encapsulating OVA-mRNA almost completely inhibited tumor growth and induced a robust cytotoxic CD8<small>⁺</small> T cell response in the spleen, outperforming clinically approved SM-102 and Dlin-MC3-DMA. Additionally, we demonstrated that YK-407 LNPs exhibited minimal toxicity for both the liver and spleen, with no significant inflammatory cytokine release. These findings highlight the potential of AI in LNP development and YK-407 holds great promise for applications in mRNA-based treatments.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 37","pages":" 11611-11620"},"PeriodicalIF":6.1,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymeric nanoparticles as a platform for nose-to-brain delivery","authors":"Marie Bolon, Morgane Marie, Simon Megy, Raphael Petrakis, Raphaël Terreux, Maxime Fieux, Claire Monge and Sophie Richard","doi":"10.1039/D5TB00947B","DOIUrl":"10.1039/D5TB00947B","url":null,"abstract":"<p >The treatment of brain disorders <em>via</em> nose-to-brain delivery (NtBD) is a promising non-invasive strategy to bypass the blood–brain barrier. In this study, we developed and characterized polymeric nanoparticles (NPs) based on polylactic acid (PLA) and poloxamers (P188 and P407), synthesized <em>via</em> one-step or two-step nanoprecipitation methods. All formulations resulted in homogeneous, negatively charged NPs with a diameter of 110 nm, compatible with efficient brain delivery. Dissipative particle dynamics simulations confirmed the structural organisation of NPs and highlighted the role of poloxamers in forming a hydrophilic surface. NPs modified with poloxamers demonstrated improved colloidal stability in artificial mucus and cerebrospinal fluid, unlike bare PLA NPs. <em>In vitro</em> assays on human nasal epithelial cells and differentiated neuronal cells showed no cytotoxicity and cellular uptake in both cell types. <em>In vivo</em> fluorescence imaging and immunostaining revealed the presence of NPs in the olfactory bulb as early as one hour post-administration. These results support NP-PLA-P188 as a promising formulation for effective and safe NtBD of drugs targeting neurological diseases.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 37","pages":" 11674-11686"},"PeriodicalIF":6.1,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}