Journal of Materials Chemistry B最新文献_第6页

Combined and rapid detection of anti-Brucella IgG/IgM in clinical samples based on surface-enhanced Raman scattering-lateral flow immunochromatography† 基于表面增强拉曼散射-侧流免疫层析技术的临床样本中抗布鲁氏菌 IgG/IgM 的联合快速检测。

IF 6.1 3区医学

Journal of Materials Chemistry B Pub Date : 2024-09-26 DOI: 10.1039/D4TB01367K

Yongwei Zhang, Ziyue Li, Aolin Zhu, Qian Zhang, Hao Zhou, Xuelei Zhou, Tingwei Liu, Chunyan Liu, Hefei Zha, Xin Zhang, Hui Zhao, Jiutong Li, Guodong Lü and Xinxia Li

{"title":"Combined and rapid detection of anti-Brucella IgG/IgM in clinical samples based on surface-enhanced Raman scattering-lateral flow immunochromatography†","authors":"Yongwei Zhang, Ziyue Li, Aolin Zhu, Qian Zhang, Hao Zhou, Xuelei Zhou, Tingwei Liu, Chunyan Liu, Hefei Zha, Xin Zhang, Hui Zhao, Jiutong Li, Guodong Lü and Xinxia Li","doi":"10.1039/D4TB01367K","DOIUrl":"10.1039/D4TB01367K","url":null,"abstract":"Brucellosis is a bacterial infectious disease caused mainly by Brucella. Transmission is mainly by contact with infected domestic or wild animals or their excreta. Clinical diagnosis of brucellosis is usually based on qualitative total antibody tests, which make it difficult to differentiate between acute and pre-existing infections. In this study, for the simultaneous detection of anti-brucellosis IgG and IgM, we innovatively developed a surface-enhanced Raman scattering (SERS)-lateral flow immunoassay (LFIA) detection system, and evaluated its performance and diagnostic effect by using clinical serum samples. The key design of this biosensor involves the preparation of two immunoprobes using two Raman spheres (R-Sphere) with distinct signals conjugated to mouse anti-human IgG and IgM, respectively. In this design, brucellosis-specific antigens are embedded in the T-line, allowing simultaneous capture of anti-brucellosis IgG and IgM in a specific binding reaction. A portable Raman instrument is used to detect the characteristic signal intensities generated by IgM and IgG on the T-line, which can then be used to analyze the target IgM and IgG antibodies. Based on the analysis of 40 clinical samples, the method has a sensitivity and specificity of 100%, and the detection time is only 15 min. The advantages of this technology are fast speed, convenient use, high sensitivity, and it can distinguish the disease course to achieve precise treatment. These features make it an ideal solution for large-scale brucellosis testing in remote and nomadic areas, where it can play a crucial role in improving public health protection.","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 42","pages":" 11012-11024"},"PeriodicalIF":6.1,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142362604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A bilayer hydrogel mimicking the periosteum-bone structure for innervated bone regeneration† 模拟骨膜-骨结构的双层水凝胶，用于神经骨再生。

IF 6.1 3区医学

Journal of Materials Chemistry B Pub Date : 2024-09-25 DOI: 10.1039/D4TB01923G

Wenhui Lyu, Yuyue Zhang, Shaopei Ding, Xiang Li, Tong Sun, Jun Luo, Jian Wang, Jianshu Li and Lei Li

{"title":"A bilayer hydrogel mimicking the periosteum-bone structure for innervated bone regeneration†","authors":"Wenhui Lyu, Yuyue Zhang, Shaopei Ding, Xiang Li, Tong Sun, Jun Luo, Jian Wang, Jianshu Li and Lei Li","doi":"10.1039/D4TB01923G","DOIUrl":"10.1039/D4TB01923G","url":null,"abstract":"In bone tissue, nerves are primarily located in the periosteum and play an indispensable role in bone defect repair. However, most bone tissue engineering approaches ignored the reconstruction of the nerve network. Herein, we aimed to develop a bilayer hydrogel simulating periosteum-bone structure to induce innervated bone regeneration. The bottom “bone” layer consisted of gelatin methacryloyl (GelMA), poly(ethylene glycol) diacrylate (PEGDA), and nano-hydroxyapatite (nHA), whereas the upper “periosteum” layer consisted of GelMA, sodium alginate (SA) and MgCl2. The mechanical properties of the upper and bottom hydrogels were designed to be suitable for neurogenesis and osteogenesis, respectively. Besides, Mg2+ from the “periosteum” layer released at the early stage (within 7 d), which aligned with the optimal time window for nerve regeneration and osteogenic related neuropeptide release. Simultaneously, the prevention of long-term Mg2+ release (after 7 d) could avoid osteogenic inhibition caused by prolonged Mg2+ exposure. Additionally, the incorporation of nHA in the bottom “bone” layer supported the long-term osteogenesis due to its osteoconductivity and slow degradation. In vitro biological experiments revealed that the bilayer hydrogel (GS@Mg/GP@nHA) promoted neurite growth and calcitonin gene-related peptide (CGRP) expression in rat dorsal root ganglion (DRG) neurons, as well as the osteogenesis of rat bone-derived mesenchymal stem cells (BMSCs). Moreover, the in vivo experiments demonstrated that the GS@Mg/GP@nHA hydrogel efficiently promoted nerve network reconstruction and bone regeneration of rat calvarial bone defects. Altogether, the bilayer hydrogel GS@Mg/GP@nHA could promote innervated bone regeneration, providing new insights for biomaterial design for bone tissue engineering.","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 43","pages":" 11187-11201"},"PeriodicalIF":6.1,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142362603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of substrate-independent heparin coating to mitigate surface-induced thrombogenesis: efficacy and mechanism† 开发与基底无关的肝素涂层，缓解表面诱导的血栓形成：功效与机制。

IF 6.1 3区医学

Journal of Materials Chemistry B Pub Date : 2024-09-25 DOI: 10.1039/D4TB01779J

Shengjun Cheng, Haifeng Ji, Tao Xu, Xianda Liu, Lin Xu, Weifeng Zhao and Changsheng Zhao

{"title":"Development of substrate-independent heparin coating to mitigate surface-induced thrombogenesis: efficacy and mechanism†","authors":"Shengjun Cheng, Haifeng Ji, Tao Xu, Xianda Liu, Lin Xu, Weifeng Zhao and Changsheng Zhao","doi":"10.1039/D4TB01779J","DOIUrl":"10.1039/D4TB01779J","url":null,"abstract":"Heparin coatings are widely applied on blood-contact materials to reduce the use of anticoagulants during blood treatment. However, the previous heparin coatings formed via covalent binding or electrostatic bonding commonly require complex surface premodification, and the blood coagulation pathway was significantly inhibited to potentially increase the bleeding risk. This contradicts the intended purpose and deviates from the anticoagulation mechanism of the heparin coatings. Herein, we present a facile and substrate-independent coating, achieved through the co-deposition of dopamine/chitosan followed by electrostatic interaction between heparin and the immobilized chitosan, which could be prepared within 1 hour. This coating prolonged the plasma re-calcification time (PRT) to over 60 minutes, effectively preventing surface-induced thrombosis. Favorable hemocompatibility was reflected in a hemolysis ratio of less than 2%, low levels of platelet adhesion and activation, and low levels of fibrinogen adhesion. We also systematically elucidate the anticoagulant mechanism of the coating, demonstrating why the coating can prevent thrombogenesis without the bleeding risk. Our work not only offers a promising and readily available heparin coating for blood-contact materials, but more importantly, the mechanism exploration supports the practical feasibility of heparin coating in various applications.","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 42","pages":" 10994-11011"},"PeriodicalIF":6.1,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects and mechanisms of berberine-loaded tetrahedral framework nucleic acids on osteoporosis 负载小檗碱的四面体框架核酸对骨质疏松症的影响和机制。

IF 6.1 3区医学

Journal of Materials Chemistry B Pub Date : 2024-09-25 DOI: 10.1039/D4TB01691B

Weifei Zhang, Chuan Guo, Dengbo Yao, Weiqiang Lan, Mi Zhou, Zhangheng Huang and Qingquan Kong

引用次数: 0

Understanding selective sensing of human serum albumin using a D–π–A probe: a photophysical and computational approach† 利用 D-π-A 探针了解人血清白蛋白的选择性感应：一种光物理和计算方法。

IF 6.1 3区医学

Journal of Materials Chemistry B Pub Date : 2024-09-25 DOI: 10.1039/D4TB01229A

Anamika Bandyopadhyay and Anupam Bhattacharya

{"title":"Understanding selective sensing of human serum albumin using a D–π–A probe: a photophysical and computational approach†","authors":"Anamika Bandyopadhyay and Anupam Bhattacharya","doi":"10.1039/D4TB01229A","DOIUrl":"10.1039/D4TB01229A","url":null,"abstract":"The human serum albumin (HSA) level is a valuable indicator of an individual's health status. Therefore, its detection/estimation can be used to diagnose several diseases. In this work, we have developed a series of donor–π–acceptor probes, which were found to selectively detect HSA over BSA (bovine serum albumin). Among these probes, A4, which bears the trifluoroacetyl group, showed the highest selectivity for HSA, with limits of detection and quantification being 1.36 nM and 2.59 nM, respectively. CD spectroscopy of the HSA–A4 ensemble indicated an increase in the α-helicity of the protein, while the displacement assays revealed the localization of the probe in the hemin site of HSA. The probe works on the principle of excited state intramolecular charge transfer (ICT). Its selectivity was also validated computationally. Docking experiments confirmed the preference of the probe for the hemin binding IB site of HSA, as observed from the fluorescence displacement assay results, and a comparison of docking scores demonstrated the greater preference of A4 for HSA compared to BSA. Computational experiments also showed a change in preference for HSA amino acid residues exhibited by the excited state of probe A4 (Tyr161, Met123, Pro118, and Leu115) when compared to its ground state (Arg186 and His146). Hydrophobic interactions dominated the excited state protein–probe ensemble, whereas there was significant involvement of the water bridges along with the hydrophobic interactions in the ground state ensemble. Probe A4 was also assessed for its practical utility and found to successfully sense HSA in urine at extremely low concentrations. Moreover, the A4–HSA ensemble was employed for hemin sensing with a detection limit of 0.23 μM.","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 41","pages":" 10719-10735"},"PeriodicalIF":6.1,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/tb/d4tb01229a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving the sulfite-detection performance of a fluorescent probe via post-synthetic modification with a metal–organic framework† 通过金属有机框架的后合成修饰提高荧光探针的亚硫酸盐检测性能。

IF 6.1 3区医学

Journal of Materials Chemistry B Pub Date : 2024-09-25 DOI: 10.1039/D4TB01754D

Jing-Yi Shi, Bin Wang, Xin-Yue Cui, Xiao-Wei Hu, Hai-Liang Zhu and Yu-Shun Yang

{"title":"Improving the sulfite-detection performance of a fluorescent probe via post-synthetic modification with a metal–organic framework†","authors":"Jing-Yi Shi, Bin Wang, Xin-Yue Cui, Xiao-Wei Hu, Hai-Liang Zhu and Yu-Shun Yang","doi":"10.1039/D4TB01754D","DOIUrl":"10.1039/D4TB01754D","url":null,"abstract":"In this work, a post-synthetic modification strategy was attempted to improve the performance of the probe for sulfite detection. The assembled platform UiO-66-NH-DQA, which was acquired by anchoring the sulfite-response fluorescent probe DQA onto the surface of UiO-66-NH2via amide covalent bonds, exhibited enhanced fluorescence intensity and practical intracellular imaging capability. In spite of the structural similarity, as verified by characterization tests, the conversion rate of post-synthetic modification was calculated as 35%, equaling an approximate assembly ratio of 1 : 2 between UiO-66-NH2 and DQA. Most significantly, conversion into UiO-66-NH-DQA led to a 5.6-fold enhancement in the reporting signal with a red shift of 20 nm. For sulfite detection, the linear range was 0–150 μM, with a limit of detection value of 0.025 μM. UiO-66-NH-DQA retained advantages including high stability (within pH 5.0–9.0), rapid response (within 15 min) and high selectivity. Based on low cytotoxicity and relatively rapid cellular uptake, UiO-66-NH-DQA achieved the imaging of both the exogenous and endogenous sulfite levels in living cells. In particular, its rapid cell-permeating capability was guaranteed during the modification. The post-synthetic modification strategy reported herein has potential for improving the practical properties of fluorescent monitoring materials.","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 43","pages":" 11251-11258"},"PeriodicalIF":6.1,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142396371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acellular dermal matrix hydrogels promote healing of radiation-induced skin injury in a rat model† 细胞真皮基质水凝胶可促进大鼠模型中辐射所致皮肤损伤的愈合。

IF 6.1 3区医学

Journal of Materials Chemistry B Pub Date : 2024-09-25 DOI: 10.1039/D4TB00941J

Xin Liu, Tian Guo, Zhifeng Huang, Sen Chen, Li Chen, Chenyang Li, Tian Tian, Yerong Qian, Lifei Yang, Junxi Xiang, Qiufang Liu and Peng Liu

{"title":"Acellular dermal matrix hydrogels promote healing of radiation-induced skin injury in a rat model†","authors":"Xin Liu, Tian Guo, Zhifeng Huang, Sen Chen, Li Chen, Chenyang Li, Tian Tian, Yerong Qian, Lifei Yang, Junxi Xiang, Qiufang Liu and Peng Liu","doi":"10.1039/D4TB00941J","DOIUrl":"10.1039/D4TB00941J","url":null,"abstract":"Background: radiation-induced skin injury (RISI) is a common complication of radiotherapy, affecting 85–95% of patients. Current treatments lack sufficient evidence of efficacy. Acellular dermal matrix (ADM) hydrogels have shown promise in treating chronic wounds, burns, and ulcers, but their potential in RISI remains unexplored. Methods: ADM hydrogels were prepared from porcine dermis and characterized using histological staining, scanning electron microscopy (SEM), and rheological assessments. A rat model of RISI was established, and the therapeutic effects of the ADM hydrogel were evaluated by gross observation, histological analysis, and immunofluorescence staining. Oxidative stress, angiogenesis, apoptosis, macrophage infiltration, and inflammatory responses were also assessed. Results: ADM hydrogel treatment significantly reduced wound area, radiation injury scores, and apoptosis while increasing epithelial thickness and hair follicle regeneration compared to the control group. The hydrogel promoted angiogenesis, vascular maturation, and M2 macrophage polarization. It also decreased the expression of pro-inflammatory cytokines (IL-1β and IL-6) and increased the expression of the anti-inflammatory cytokine IL-10. No significant differences in antioxidant effects were observed between the groups. Conclusion: The ADM hydrogel effectively promotes the healing of RISI in a rat model by modulating the inflammatory microenvironment and enhancing angiogenesis. These findings suggest that the ADM hydrogel could serve as a promising novel biomaterial for the management of RISI.","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 43","pages":" 11218-11229"},"PeriodicalIF":6.1,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142383012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Bioreducible and acid-labile polydiethylenetriamines with sequential degradability for efficient transgelin-2 siRNA delivery 更正：可生物再现的酸性可降解聚二乙烯三胺，用于高效递送转胰岛素-2 siRNA。

IF 6.1 3区医学

Journal of Materials Chemistry B Pub Date : 2024-09-24 DOI: 10.1039/D3TB90091F

Pengchong Wang, Yan Yan, Ying Sun, Rui Zhang, Chuanchuan Huo, Lu Li, Ke Wang, Yalin Dong and Jianfeng Xing

引用次数: 0

ROS/pH dual-responsive quercetin-loaded guanosine borate supramolecular hydrogel enema in dextran sulfate sodium-induced colitis in mice ROS/pH 双响应槲皮素负载硼酸鸟苷超分子水凝胶灌肠剂在葡聚糖硫酸钠诱导的小鼠结肠炎中的应用

IF 6.1 3区医学

Journal of Materials Chemistry B Pub Date : 2024-09-24 DOI: 10.1039/D4TB01659A

Luqing Zhao, Dan Dou, Di Zhang, Xin Deng, Ning Ding, Yun Ma, Xingyu Ji, Shengsheng Zhang and Chao Li

{"title":"ROS/pH dual-responsive quercetin-loaded guanosine borate supramolecular hydrogel enema in dextran sulfate sodium-induced colitis in mice","authors":"Luqing Zhao, Dan Dou, Di Zhang, Xin Deng, Ning Ding, Yun Ma, Xingyu Ji, Shengsheng Zhang and Chao Li","doi":"10.1039/D4TB01659A","DOIUrl":"10.1039/D4TB01659A","url":null,"abstract":"Ulcerative colitis (UC) is an inflammatory bowel disease that predominantly impacts the colon, typically starting in the rectum. A significant characteristic of UC is its propensity to affect the distal colon, which is particularly beneficial for targeted treatments such as enemas. This localized approach ensures that the medication is delivered directly to the affected areas, resulting in minimal systemic absorption. In this research, we have formulated a novel stimuli-responsive quercetin-loaded guanosine borate supramolecular hydrogel (named GBQ hydrogel), designed to prolong the residence time of the drug and protect the ulcerated intestinal tissues. The GBQ hydrogel has exhibited excellent injectability, self-healing capabilities, and biocompatibility, rendering it an ideal candidate for enema administration. In vitro studies have highlighted its ROS/pH dual-responsive release profile, which mimics the microenvironment of intestinal inflammation. Furthermore, we assessed the efficacy of the GBQ hydrogel on dextran sulfate sodium (DSS)-induced colitis, a common animal model for UC. Our findings indicate that the GBQ hydrogel significantly reduces disease activity, mitigates oxidative stress, restores the intestinal mucosal barrier, and prevents colonic cell apoptosis. Collectively, this study underscores the therapeutic potential of the GBQ hydrogel in managing inflammatory bowel conditions and paves the way for a novel hydrogel enema-based treatment strategy for UC.","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 42","pages":" 10861-10876"},"PeriodicalIF":6.1,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142368055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A near-infrared superoxide generator based on a biocompatible indene-bearing heptamethine cyanine dye† 一种基于生物相容性茚满七甲叉氰染料的近红外超氧化物发生器。

IF 6.1 3区医学

Journal of Materials Chemistry B Pub Date : 2024-09-24 DOI: 10.1039/D4TB01663G

Nikita Bel’ko, Anna Mal’tanova, Anastasiya Bahdanava, Anatol Lugovski, Sviatlana Fatykhava, Polina Shabunya, Adam Smaliakou, Sergey Poznyak, Tatsiana Kulahava and Michael Samtsov

{"title":"A near-infrared superoxide generator based on a biocompatible indene-bearing heptamethine cyanine dye†","authors":"Nikita Bel’ko, Anna Mal’tanova, Anastasiya Bahdanava, Anatol Lugovski, Sviatlana Fatykhava, Polina Shabunya, Adam Smaliakou, Sergey Poznyak, Tatsiana Kulahava and Michael Samtsov","doi":"10.1039/D4TB01663G","DOIUrl":"10.1039/D4TB01663G","url":null,"abstract":"One of the most significant limitations of photodynamic therapy is its reduced efficacy in hypoxic microenvironments, which are typical of the majority of tumors. This work demonstrates that indolenine heptamethine cyanines with different substituents in the polymethine chain and at the terminal heterocycles are effective superoxide generators that can be activated in the near-infrared range. The introduction of an indene moiety into the polymethine chain results in a significant enhancement in photostability compared to dyes with a cyclohexene moiety or an unsubstituted polymethine chain. A hydrophilic indene-bearing heptamethine cyanine dye is shown to be efficiently internalized by Vero E6 cells and to give bright intracellular fluorescence in the 700–850 nm range. Furthermore, the dye generates superoxide anion radicals and induces severe oxidative stress in cells upon activation in the near-infrared range (∼750 nm), ultimately resulting in cell death. The capacity of heptamethine cyanines to generate a superoxide anion radical may prove advantageous for enhancing the efficacy of photodynamic therapy under hypoxic conditions.","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 43","pages":" 11202-11209"},"PeriodicalIF":6.1,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142373940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0