Journal of Materials Chemistry B最新文献

{"title":"NAD+ modulation with nicotinamide mononucleotide coated 3D printed microneedle implants†","authors":"Masood Ali, Wenhao Huang, Yicheng Huang, Xiaoxin Wu, Sarika Namjoshi, Indira Prasadam, Heather A. E. Benson, Tushar Kumeria and Yousuf Mohammad","doi":"10.1039/D4TB01856G","DOIUrl":"10.1039/D4TB01856G","url":null,"abstract":"<p >Nicotinamide adenine dinucleotide (NAD<small>⁺</small>) deficiency has been shown to cause pathogenesis of age-related functional decline and diseases. Investigational studies have demonstrated improvements in age-associated pathophysiology and disease conditions. However, invasive methods such as immunohistochemistry, metabolic assays, and polymerase chain reaction currently used to measure cell metabolism render cells unviable and unrecoverable for longitudinal studies and are incompatible with <em>in vivo</em> dynamic observations. We report a non-invasive optical technique to investigate the upregulation of nicotinamide adenine dinucleotide (NAD<small>⁺</small>) in keratinocytes (both <em>in vitro</em> and <em>ex vivo</em>) upon administration of nicotinamide mononucleotide (NMN) coated microneedle (μNDs) implants. Our technique exploits intrinsic autofluorescence of cells and tissues using multiphoton microscopy. Additionally, μND coating formulations to date have been evaluated using fluorescence microscopy to determine the coated amount, often an imprecise correlation between fluorescence intensity and the coated amount on the μND surface. We also show that rheomechanical attributes of the coating formulation (containing two different viscosity enhancers: sucrose and carboxy methyl cellulose) affect the flow mechanics of the coating formulation at micron scale, and thus the amount of drug coated on the μND surface. <em>In vitro</em> keratinocyte cells were investigated with four concentrations of NMN (50, 250, 500 and 1000 μg), and evaluated with time-dependent NMN (500 μg) treatment at 0, 5, 10, 30, 60, 360 and 1460 min. We demonstrate that intracellular keratinocyte fluorescence of the endogenous NADH shows a decreasing trend in both the average fluorescence lifetime (<em>τ</em><small>_m</small>) and the free unbound NADH (<em>τ</em><small>₁</small>), with increasing dosage of NMN administration. A similar trend in the average fluorescence lifetime (<em>τ</em><small>_m</small>) of endogenous NAD(P)H was also seen in mouse ear skin <em>ex vivo</em> skin upon administration of NMN. We show a promising, minimally invasive, alternative delivery system for the NAD<small>⁺</small> precursor molecule that can enhance patient compliance and therapeutic outcomes.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 11","pages":" 3564-3580"},"PeriodicalIF":6.1,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143416570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A liquid metal-based sticky conductor for wearable and real-time electromyogram monitoring with machine learning classification†","authors":"Zixin Lin, Mingmei Luo, Jiayi Liang, Zijie Li, Yanting Lin, Xiaman Chen, Baozhu Chen, Liang Peng, Yongchang Ouyang and Lei Mou","doi":"10.1039/D4TB01711K","DOIUrl":"10.1039/D4TB01711K","url":null,"abstract":"<p >Skin electronics face challenges related to the interface between rigid and soft materials, resulting in discomfort and signal inaccuracies. This study presents the development and characterization of a liquid metal–polydimethylsiloxane (LM–PDMS) sticky conductor designed for wearable electromyography (EMG) monitoring. The conductor leverages a composite of LM inks and PDMS, augmented with silver nanowires (AgNWs) and surface-modified with mercaptoundecanoic acid (MUD) to enhance conductivity. The mechanical properties of the PDMS matrix were optimized using Triton-X to achieve a flexible and adhesive configuration suitable for skin contact. Our LM–PDMS sticky conductor demonstrated excellent stretchability, could endure up to 300% strain without damage, and maintained strong adherence to the skin without relative displacement. Biocompatibility tests confirmed high cell viability, making it suitable for long-term use. EMG signal analysis revealed reliable muscle activity detection, with advanced signal processing techniques effectively filtering noise and stabilizing the baseline. Furthermore, we employed machine learning algorithms to classify EMG signals, achieving high accuracy in distinguishing different muscle activities. This study showcases the potential of LM–PDMS sticky conductors for advanced wearable bioelectronics, offering promising applications in personalized healthcare and real-time muscle activity monitoring.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 12","pages":" 3906-3917"},"PeriodicalIF":6.1,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143506677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organ-targeted drug delivery systems (OTDDS) of poly[(N-acryloylglycine)-co-(N-acryloyl-l-phenylalanine methyl ester)] copolymer library and effective treatment of triple-negative breast cancer†","authors":"Sukanya Patra, Jyotirmayee, Krishan Kumar, Divya Pareek, Prem Shankar Gupta, Anjali Ramsabad Mourya, Taniya Das, Kirti Wasnik, Malkhey Verma, Ruchi Chawla, Tarun Batra and Pradip Paik","doi":"10.1039/D4TB02445A","DOIUrl":"10.1039/D4TB02445A","url":null,"abstract":"<p >Organ-targeted drug delivery systems (OTDDS) are essential for the effective treatment of complicated diseases. Triple-negative breast cancer (TNBC) is an aggressive cancer with high mortality and requires targeted therapeutics. This work was aimed at designing a library of polymeric OTDDS with <em>N</em>-acryloyl-glycine (NAG) and <em>N</em>-acryloyl-<small>L</small>-phenylalanine methyl ester (NAPA) [p(NAG-<em>co</em>-NAPA)<small>_{(<em>x</em>:<em>y</em>)}</small>] and screening its <em>in vivo</em> organ-targeting specificity. Among this library, the best p(NAG-<em>co</em>-NAPA)<small>_{(<em>x</em>:<em>y</em>)}</small> NPs with an <em>x</em> : <em>y</em> ratio of 1 : 4 and size of 160–210 nm targeted breasts to a high extent compared to other organs and thus were optimized for TNBC treatment. A network pharmacology study was performed, which revealed that 14 genes were responsible for TNBC, and a combination of DHA (targets 6 genes) and piperine (targets 8 genes) drugs was used to optimize the formulation, achieving the maximum therapeutic efficiency against TNBC with an IC<small>₅₀</small> value of 350 μg mL<small>⁻¹</small>. The designed organ-specific polymeric nanoparticle (NP) library, identification of target genes and proteins responsible for TNBC, and the optimized formulation for effective combination therapy established an effective therapeutic option for TNBC. The findings of this work further demonstrate that this polymeric library of NPs shows exciting therapeutic potential for treating TNBC and presents innovative treatment options for critical diseases of the liver, heart, lungs and kidney.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 12","pages":" 3876-3894"},"PeriodicalIF":6.1,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d4tb02445a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in 3D gel culture systems for enhanced angiogenesis in bone tissue engineering","authors":"Aijing Shi, Yixin Shi, Jie Li, Minghan Ye, Xiaoqing Ma, Yuke Peng, Kuo Gai and Junyu Chen","doi":"10.1039/D4TB01139B","DOIUrl":"10.1039/D4TB01139B","url":null,"abstract":"<p >Angiogenesis–osteogenesis coupling is a crucial process in bone tissue engineering, requiring a suitable material structure for vessel growth. Recently, the 3D culture system has gained significant attention due to its benefits in cell growth, proliferation and tissue regeneration. Its most notable advantage is its ECM-like function, which supports endothelial cell adhesion and facilitates the formation of vascular-like networks—crucial for angiogenesis–osteogenesis coupling. Hydrogels, with their highly hydrophilic polymer network resembling the extracellular matrix, make the 3D gel culture system an ideal approach for angiogenesis due to its cellular integrity and adjustable properties. This article reviews the current use of 3D gel culture systems in bone tissue engineering, covering substrates, characteristics and processing technologies, thereby offering readers profound insights into these systems.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 11","pages":" 3516-3527"},"PeriodicalIF":6.1,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unleashing the antibacterial potential of ZIFs and their derivatives: mechanistic insights","authors":"Geetika Jain, Radhika Chaurasia, Bani Preet Kaur, Ontar Paul Chowdhury, Hiranmay Roy, Richa Rani Gupta, Bhaskar Biswas, Sandip Chakrabarti and Monalisa Mukherjee","doi":"10.1039/D4TB02682A","DOIUrl":"10.1039/D4TB02682A","url":null,"abstract":"<p >Antibiotic resistance presents an alarming threat to global health, with bacterial infections now ranking among the leading causes of mortality. To address this escalating challenge, strategies such as antibiotic stewardship, development of antimicrobial therapies, and exploration of alternative treatment modalities are imperative. Metal–organic frameworks (MOFs), acclaimed for their outstanding biocompatibility and <em>in vivo</em> biodegradability, are promising avenues for the synthesis of novel antibiotic agents under mild conditions. Among these, zeolitic imidazolate frameworks (ZIFs), a remarkable subclass of MOFs, have emerged as potent antibacterial materials; the efficacy of which stems from their porous structure, metal ion content, and tunable functionalized groups. This could be further enhanced by incorporating or encapsulating metal ions, such as Cu, Fe, Ti, Ag, and others. This perspective aims to underscore the potential of ZIFs as antibacterial agents and their underlying mechanisms including the release of metal ions, generation of reactive oxygen species (ROS), disruption of bacterial cell walls, and synergistic interactions with other antibacterial agents. These attributes position ZIFs as promising candidates for advanced applications in combating bacterial infections. Furthermore, we propose a novel approach for synthesizing ZIFs and their derivatives, demonstrating exceptional antibacterial efficacy against <em>Escherichia coli</em> and <em>Staphylococcus aureus</em>. By highlighting the benefits of ZIFs and their derivatives as antibacterial agents, this perspective emphasizes their potential to address the critical challenge of antibiotic resistance.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 10","pages":" 3270-3291"},"PeriodicalIF":6.1,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143401007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-responsive polydopamine-embellished Zn-MOFs enabling synergistic photothermal and antibacterial metal ion therapy for oral biofilm eradication†","authors":"Ge Pan, Jinyao Zheng, Zongjia Li, Qian Duan, Miaomiao Zhang and Dapeng Wang","doi":"10.1039/D4TB02427C","DOIUrl":"10.1039/D4TB02427C","url":null,"abstract":"<p >Oral biofilms are associated with various oral diseases causing pain and discomfort, and pose a severe threat to general health. Conventional surgical debridement and antibacterial therapy often yield unsatisfactory outcomes because they either fail to fully and painlessly eliminate biofilms or increase the risk of bacterial resistance. In this study, we synthesized polydopamine-embellished Zn-MOFs (ZIF-8@PDA NPs), which can degrade under mildly acidic conditions to release Zn<small>²⁺</small>. These nanoparticles also convert near-infrared light energy into heat, thereby enabling synergistic photothermal and antibacterial metal ion therapy for oral biofilm eradication. Our findings reveal that therapy with ZIF-8@PDA NPs, when exposed to near-infrared radiation, demonstrates exceptional antibacterial efficacy and is highly effective in eradicating oral biofilms both <em>in vitro</em> and <em>ex vivo</em>. Furthermore, we used an <em>in vivo</em> rodent tooth biofilm model to demonstrate the suppression of dental caries. This work presents a promising solution for preventing and suppressing dental caries as well as other treating diseases linked to oral biofilm infections.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 11","pages":" 3730-3743"},"PeriodicalIF":6.1,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A light-driven molecular motor-polypeptide conjugate supports controlled cell uptake†","authors":"Camilla Pegoraro, Ainoa Guinart, Esther Masiá Sanchis, Daniel Doellerer, Marc C. A. Stuart, Inmaculada Conejos-Sánchez, Ben L. Feringa and María J. Vicent","doi":"10.1039/D4TB02434F","DOIUrl":"10.1039/D4TB02434F","url":null,"abstract":"<p >While light-driven molecular motors (<strong>MMs</strong>) hold immense potential to control cell function, low biocompatibility and solubility have hampered their implementation. We developed a novel polypeptide-conjugated <strong>MM</strong> by linking a propargyl-derivatized light-driven <strong>MM</strong> to a poly-<small>L</small>-glutamic acid-based carrier (<strong>P</strong>) with inherent mitochondria tropism through click chemistry, denoted <strong>P-MM</strong>. <strong>P-MM</strong> effectively maintained the parental stability and unidirectional rotational capabilities of <strong>MM</strong> upon irradiation at 405 nm. Light-induced supramolecular conformational changes significantly increased cell uptake compared to non-irradiated controls while retaining the subcellular targeting capacity of <strong>P</strong>. <strong>P-MM</strong> exhibited minimal cytotoxicity and reactive oxygen species production, suggesting a non-disruptive interaction with cell membranes. Overall, we establish a connection between irradiation and enhanced biological responses, demonstrating the potential of integrating <strong>MMs</strong> with targeted polymeric nanocarriers for controlled, light-responsive behavior in biological systems and innovative applications in advanced therapeutic/diagnostic strategies.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 8","pages":" 2658-2665"},"PeriodicalIF":6.1,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d4tb02434f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143392826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atomically dispersed copper(i) on tungstosilicic acid for catalytic protection against cisplatin-induced hearing loss†","authors":"Xiaochan Lu, Yin Chen, Yanmei Mo, Qingdong Zeng, Shaoqin Cen, Li Zeng, Hongyi Hu, Ao Li, Xia Gao and Bin Zhang","doi":"10.1039/D4TB02820A","DOIUrl":"10.1039/D4TB02820A","url":null,"abstract":"<p >The employment of platinum-based drugs for cancer chemotherapy, which might yield oxidative stress, is regarded as one main factor leading to hearing loss. The exact molecular mechanisms for cisplatin-induced hearing loss require further clarification, thus limiting the development of FDA-approved therapies. Herein, we mimicked the molecular structure of natural antioxidative enzymes to fabricate a four-oxygen-coordinating copper single-atom nanozyme (Cu SAN) exhibiting good superoxide dismutase and catalase activity, to alleviate the oxidative stress induced by platinum-based drugs. Notably, Cu SAN exhibited profound protective effects against cisplatin-induced hair cell damage with only 15 ng mL<small>⁻¹</small> of Cu species, successfully reversing cisplatin-induced hearing loss <em>via</em> oral administration. Due to its oxidation resistance, pretreatment with Cu SAN significantly improved cell viability and reduced ROS accumulation in cisplatin-triggered hair cell damage in HEI-OC1 cells and cochlear explants. Our results first demonstrated that cisplatin treatment induced cuproptosis in hair cells by modulating copper ion homeostasis. Further investigation revealed that Cu SAN nanozyme effectively alleviated hair cell cuproptosis by regulating FDX1 and reducing aggregated lipoacylated protein. This research underscores the promising potential of four-oxygen-coordinating Cu nanomaterials as a therapeutic approach to combat hearing loss, providing a new strategy for auditory protection.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 11","pages":" 3540-3552"},"PeriodicalIF":6.1,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143485095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A thermoresponsive PEG-based methacrylate triblock terpolymer as a bioink for 3D bioprinting†","authors":"Kaiwen Zhang, Anna P. Constantinou, Cathal O’Connell, Theoni K. Georgiou and Amy Gelmi","doi":"10.1039/D4TB02572E","DOIUrl":"10.1039/D4TB02572E","url":null,"abstract":"<p >Thermoresponsive polymers have been extensively reported for their use in tissue engineering and drug delivery applications. They have a wide range of thermoresponsive and rheological properties controlled by their structural characteristics, such as composition and architecture. Here, the considerable potential of a PEG based, non-ionic triblock thermoresponsive copolymer, namely OEGMA300<small>₁₃</small>-<em>b</em>-BuMA<small>₂₂</small>-<em>b</em>-DEGMA<small>₁₂</small> as a bioink for 3D printing with cell encapsulation is identified. The rheological tests showed that the gel transition temperature is 8 °C with 35% w/w concentration in PBS. The printability and cytotoxicity of the thermoresponsive gel were characterised and compared with those of commercial thermoresponsive polymer Pluronic®F127 in detail. Specifically, the 35% w/w triblock copolymer presented great printability with a printing speed of 450 mm min<small>⁻¹</small> at 37 °C, and was less cytotoxic than F127 at both 20% and 30% w/w concentrations. A one-layer structure of human mesenchymal stem cell (hMSC) embedded triblock copolymer was successfully printed onto a glass slide at 37 °C. This provides an option to create a scaffold for stem cell culture and programming for further tissue engineering applications <em>via</em> direct printing of a cell-laden thermoresponsive polymer.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 11","pages":" 3593-3601"},"PeriodicalIF":6.1,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d4tb02572e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143461266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decomposable STING nanoagonist-amplified oncolytic virotherapy through remodeling the immunosuppressive microenvironment of triple-negative breast cancer†","authors":"Min Mu, Guoqing Wang, Bo Chen, Hui Li, Chenqian Feng, Rangrang Fan, Nianyong Chen, Bo Han, Aiping Tong, Bingwen Zou and Gang Guo","doi":"10.1039/D4TB02565B","DOIUrl":"10.1039/D4TB02565B","url":null,"abstract":"<p >Oncolytic viruses (OVs) are promising for cancer treatment as they specifically replicate in tumor cells. However, the systemic delivery of OVs still faces the challenges of poor tumor targeting, short circulation periods, and limited lytic efficacy. Herein, an OV-concealed targeting nanoagonist (OV-MnO<small>₂</small>/HE) was prepared to enhance the delivery of OVs to triple-negative breast cancer (TNBC) <em>via</em> intravenous administration. Decomposable MnO<small>₂</small> biomineral shells covered the surface antigens of OVs to prevent their clearance after systemic administration. The targeting materials of HA-EGCG (HE) enhanced intratumoral accumulation <em>via</em> active targeting. After entering tumors, OV-MnO<small>₂</small>/HE readily released Mn<small>²⁺</small> and OVs, which could enhance the number of CD4<small>⁺</small>/CD8<small>⁺</small> T cells and maturation dendritic cells (DCs) due to the synergetic effect of the STING pathway and OVs, thereby activating the immune response, resulting in the significant inhibition of TNBC growth. This work highlights the potential of the STING agonist in enhancing the antitumor efficacy of OVs and provides a potent platform for TNBC therapy.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 11","pages":" 3685-3699"},"PeriodicalIF":6.1,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143470440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}