Journal of Materials Chemistry B最新文献

{"title":"Efficient release of COS/H2S via BODIPY-based photocaged thionocarbamates","authors":"Yanmei Li, Emily Kryvorutsky, Yuanwei Zhang and Pier Alexandre Champagne","doi":"10.1039/D5TB01313E","DOIUrl":"10.1039/D5TB01313E","url":null,"abstract":"<p >The controlled generation and delivery of hydrogen sulfide (H<small>₂</small>S), a critical gasotransmitter involved in various physiological and therapeutic processes, remain significant challenges, driving the need for innovative and responsive chemical tools. In this study, we developed thionocarbamate derivatives based on the boron dipyrromethene (BODIPY) scaffold, which rapidly release carbonyl sulfide (COS) upon photoactivation, allowing H<small>₂</small>S generation under physiological conditions. These systems exhibit an exceptional uncaging rate compared with other reported structures, enabling rapid and precisely controlled H<small>₂</small>S releases, as verified through live-cell imaging studies. In particular, the photolysis rate of the thionocarbamate derivatives was found to be more than 100-fold faster than the corresponding carbamates, a result that is explained through density functional theory (DFT) calculations as arising from the thermodynamic instability of thiocarbonyls, offering a new strategy in photocage design. These results also highlight the potential of using thionocarbamate-based BODIPY derivatives as versatile tools for H<small>₂</small>S delivery, paving the way for their application in H<small>₂</small>S-related therapies and studies.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 35","pages":" 11046-11054"},"PeriodicalIF":6.1,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144877638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inorganic–organic γ-octamolybdate hybrids for targeted anticancer applications against MiaPaca-2 and A549 cells","authors":"Ankita Pardiwala, Nandini Bajaj, Arup Kumar Ghosh, Deepika Sharma, Komal Kumar Jangir and Ritambhara Jangir","doi":"10.1039/D5TB01422K","DOIUrl":"10.1039/D5TB01422K","url":null,"abstract":"<p >Cancer is widely recognized as one of the most critical public health challenges, transcending economic boundaries and impacting populations across all socioeconomic strata. Developing effective cancer therapies is significantly hindered by challenges such as chemotherapy-related side effects, drug resistance, and tumor metastasis, which contribute to poor prognoses for many patients. In this context, inorganic drugs, particularly polyoxomolybdate-based inorganic–organic hybrids, are emerging as promising candidates for future metallodrugs. In this study, we report the synthesis of inorganic–organic γ-octamolybdate hybrids, [(C1bipy)<small>²⁺</small>(DMA)<small>²⁺</small>][(Mo<small>₈</small>O<small>₂₆</small>)<small>⁴⁻</small>]·H<small>₂</small>O (<strong>1</strong>) and [(2,4,6-TMPY)<small>₂</small><small>⁺</small>(DMA)<small>₂</small><small>⁺</small>][Mo<small>₈</small>O<small>₂₆</small>]<small>⁴</small><strong><small>⁻</small></strong> (<strong>2</strong>), and characterization by a combined experimental and computational study. The molecular structures of these hybrids were elucidated using single-crystal X-ray diffraction techniques and Hirshfeld analyses. The materials exhibit remarkable stability in aqueous media and demonstrate low toxicity toward normal cell lines. The <em>in vitro</em> cytotoxicity of γ-octamolybdate-based hybrid solids (<strong>1</strong> and <strong>2</strong>) was systematically evaluated against mammalian pancreatic (MiaPaca-2) and lung (A549) cancer cell lines, revealing their unprecedented potency. <strong>1</strong> exhibited IC<small>₅₀</small> values of 1.3–2.5 μM for A549 and 3.7–4.1 μM for MiaPaca-2 cells, similarly <strong>2</strong> exhibited exceptional activity, with IC<small>₅₀</small> values of 1.3–2.5 μM for MiaPaca-2 and 4.1–4.5 μM for A549 cells. Both materials achieved up to 90% inhibition of cell viability at 13 μM, significantly surpassing prior benchmarks. Mechanistic investigations <em>via</em> cell cycle analysis elucidated G1 phase arrest as the pivotal mode of anticancer action, disrupting cellular proliferation with high specificity and potency. These findings evidenced that γ-[Mo<small>₈</small>O<small>₂₆</small>]<small>⁴⁻</small> hybrids act as robust candidates for therapeutic applications, offering a transformative approach to overcome current limitations in oncological interventions. Thus, this study constitutes the inaugural exploration of γ-octamolybdate-based hybrid materials in anticancer therapy, underscoring their potential for addressing malignancies, particularly pancreatic and lung cancers, at exceptionally low effective concentrations.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 38","pages":" 12185-12204"},"PeriodicalIF":6.1,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144994936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective detection of an antibiotic using hydrogen-bonded organic frameworks (HOFs): insights from DFT mechanistic analysis","authors":"Malabika Ghosh, Uddipan Dasgupta, Prashanth Venkatesan, Manas Kumar Sarangi, Rupali Gangopadhyay, Ruey-An Doong and Ankan Dutta Chowdhury","doi":"10.1039/D5TB01528F","DOIUrl":"10.1039/D5TB01528F","url":null,"abstract":"<p >The development of non-toxic, cost-effective and high fluorescent sensing materials has earned significant interest in the last decade. In this work, a simple synthesis technique of mesoporous hydrogen-bonded organic frameworks (HOFs) suitable for the ultrasensitive detection of a commonly used antibiotic, enrofloxacin (ENR), has been reported. The fluorescence of the HOF is completely quenched after the formation of a HOF–Cu<small>²⁺</small> complex as a turn off sensor which undergoes a turn-on mechanism in the presence of ENR. The competitive binding of ENR displaces Cu<small>²⁺</small> ions, thereby restoring the fluorescence of the free HOF. The quenching effect of the fluorescence of the HOF in the presence of Cu<small>²⁺</small> ions was quantitatively analysed, establishing a limit of detection (LOD) of 57 nM. The formation of a Cu<small>²⁺</small>–ENR conjugate upon ENR addition leads to the recovery of fluorescence intensity. The mechanism of competitive binding was validated by kinetic studies and computational studies based on density functional theory (DFT). The sensor demonstrated a linear response for ENR detection between 0.01 and 1.0 μM, with a limit of detection (LOD) of 70 nM, and a wider non-linear detection range extending to 50 μM. Furthermore, successful recovery tests in spiked buffer, diluted human serum, commercial milk, and river water samples confirmed the robustness of the sensing platform in complex biological and environmental matrices. The high sensitivity with excellent selectivity towards the antibiotic in the presence of other interferences and the successful recovery in spiked sample analysis highlight the potential applications of this sensing platform.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 38","pages":" 12154-12165"},"PeriodicalIF":6.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multifunctional electrospun nanofiber/hydrogel-based pro-healing bilayer dressing as a next generation biomaterial for skin wound care","authors":"Dimpy Bhardwaj, Vatan Chawla, Vanshika Nandwani, Yashika Thakur, Yashveer Singh and Garima Agrawal","doi":"10.1039/D5TB00800J","DOIUrl":"10.1039/D5TB00800J","url":null,"abstract":"<p >Infectious wounds present a significant challenge in healthcare due to the delay in wound healing and associated processes. Improper use of antibiotics makes this situation even worse due to antibiotic resistance. To meet the critical requirements of healing infectious wounds, we report a bilayer dressing (BL) that combines a hydrogel-based layer and an electrospun nanofiber-based layer together to mimic the dermal and epidermal architecture of normal skin. The bilayer dressing is fabricated by combining a chitosan/gelatin nanofiber-based layer (NF) with an ursodeoxycholic acid drug (UDC) and carbon dot (CD) loaded hydrogel (UDC/CDs/H-Gel). The hydrogel is fabricated by Schiff base-based crosslinking of quaternized chitosan (QCS) and oxidized alginate (OA). The integration of NF with UDC/CDs/H-Gel leads to ∼45% increment in tensile strength and ∼48% increment in elongation at break. The BL exhibits a swelling of ∼400% in 36 h, a porosity of ∼75%, and an antioxidant activity of ∼93%. Moreover, as compared to individual NF and hydrogel layers, the BL shows good reactive oxygen species (ROS) scavenging behavior, good hemocompatibility (∼4.5% hemolysis), good hemostatic potential, enhanced cell proliferation ability (130% cell viability of L929 cells), and excellent antibacterial activity with 92% and 88% bactericidal efficacy against <em>E. coli</em> and <em>S. aureus</em>, respectively. The wound healing ability of the BL is further evaluated <em>via</em> scratch assay demonstrating ∼97% wound closure. Overall, the BL possesses multifunctionality and presents itself as a potential candidate for accelerated wound healing.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 37","pages":" 11821-11834"},"PeriodicalIF":6.1,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d5tb00800j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reversible photo-induced formation of iron alginate hydrogels","authors":"Alexey S. Sokolov, Arkady S. Abdurashitov, Pavel I. Proshin and Gleb B. Sukhorukov","doi":"10.1039/D5TB01457C","DOIUrl":"10.1039/D5TB01457C","url":null,"abstract":"<p >Sodium alginate is well-known to be crosslinked by various polyvalent metal ions. While calcium ions (Ca<small>²⁺</small>) have been the most used, the crosslinking of alginate with other metal ions has received much less attention in the literature. For instance, Fe<small>²⁺</small> and Fe<small>³⁺</small> ions can also crosslink sodium alginate, though with varying strengths. A change in the charge of the iron ion can significantly affect the hydrogel's crosslinking density, potentially leading to full dissolution. This study demonstrates a novel approach to reversibly control alginate hydrogel formation and dissolution using visible light as an external stimulus. Visible light irradiation (450 nm) leads to the decomposition of the iron-containing sandwich complex (ISC). Liberated Fe<small>²⁺</small> ions undergo quick oxidation by potassium peroxydisulfate and the resulting Fe<small>³⁺</small> ions crosslink alginate chains to form a hydrogel. Conversely, treatment with 405 nm visible light induces a redox reaction between lactic acid and Fe<small>³⁺</small> ions. The recovery of Fe<small>³⁺</small> into Fe<small>²⁺</small> leads to the hydrogel's full de-crosslinking and reversion to a solution. Notably, this process can be performed in a single step <em>via</em> visible light irradiation. The photochemical processes rapidly lead to gelation and re-gelation occurring within minutes. Envisaged applications of reversible photo-induced gelation are under discussion.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 38","pages":" 12166-12171"},"PeriodicalIF":6.1,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imparting new stimuli-responsive behaviors in protein–polymers via self-immolative linker conjugation","authors":"Federico Kaufman, Maya David, Michal Zaiden, Doron Shabat and Miriam Amiram","doi":"10.1039/D5TB01334H","DOIUrl":"10.1039/D5TB01334H","url":null,"abstract":"<p >The development of “smart” polymers capable of responding to physiologically relevant stimuli is essential for engineering dynamic sensing and actuation systems that leverage biological signals under specific (patho)physiological conditions. In this study, we present a general and versatile strategy to engineer novel stimuli-responsive behaviors in temperature-responsive protein-based polymers (PBPs) <em>via</em> site-specific conjugation with self-immolative molecules. Specifically, we developed hydrogen peroxide (H<small>₂</small>O<small>₂</small>)- and β-galactosidase (β-gal)-responsive elastin-like polypeptides (ELPs) and resilin-like polypeptides (RLPs). Using a library of ELPs with varying numbers of conjugation sites, we demonstrate that this approach enables precise modulation of stimulus-responsive phase transitions, providing a tunable temperature window of up to 50 °C for stimuli-controlled phase transition. We further show that incorporation of these responsive ELPs into collagen hydrogels allows for controlled, dose- and time-dependent release of the ELPs, accompanied by stimulus-induced changes in the hydrogel's transparency, and storage and loss moduli. Additionally, we engineered diblock copolymer nanostructures comprising ELP–ELP or RLP–ELP segments for encapsulation and stimulus-triggered release of a hydrophobic model payload (Nile red) with varying release profiles. Together, these results establish a robust platform for imparting environmentally responsive functionalities to PBPs by integrating recombinant synthesis with chemically triggered actuation, thereby enabling the rational design of adaptive biomaterials with tunable physicochemical and biological properties for a wide range of biomedical and biotechnological applications.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 38","pages":" 12276-12292"},"PeriodicalIF":6.1,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145002376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative diopside–MnFe2O4 nanocomposites: a multifunctional platform for bone regeneration and hyperthermia therapy featuring MnFe2O4 nanoparticles with near-bulk magnetic performance","authors":"Guan-Xiang Liao, Wei-Hsi Chang, Yu-Sheng Tseng and Wen-Fan Chen","doi":"10.1039/D5TB00860C","DOIUrl":"10.1039/D5TB00860C","url":null,"abstract":"<p >This study explores the novel integration of MnFe<small>₂</small>O<small>₄</small> nanoparticles into diopside bioceramics, paving the way for advanced multifunctional nanocomposites tailored for orthopedic and oncological applications. Diopside is synthesized using biowaste-derived eggshells and rice husks <em>via</em> solid-state reaction at an optimal sintering temperature of 1200 °C. MnFe<small>₂</small>O<small>₄</small> nanoparticles, with an average particle size of 46 nm, are produced through a facile hydrothermal method coupled with magnetic separation, achieving an impressive saturation magnetization (<em>M</em><small>_s</small>) of 81.6 emu g<small>⁻¹</small> (99.5% of bulk MnFe<small>₂</small>O<small>₄</small>) – the highest reported to date. This exceptional performance is attributed to the nanoparticles’ excellent crystallinity, single-domain behavior, and minimized surface effects. Incorporating MnFe<small>₂</small>O<small>₄</small> nanoparticles into diopside significantly enhances the sinterability, density, and hardness by 2–2.5 times while reducing porosity to ∼1%. Even at a low addition of 10 wt% MnFe<small>₂</small>O<small>₄</small>, the nanocomposites demonstrate effective hyperthermia within a safe therapeutic range (41–46 °C) under an alternating magnetic field, with negligible coercivity and remanence. Biocompatibility evaluations confirm no cytotoxicity and reveal enhanced osteoblast differentiation and mineralization. This study successfully synthesizes MnFe<small>₂</small>O<small>₄</small> nanoparticles with near-bulk saturation magnetization and highlights diopside–MnFe<small>₂</small>O<small>₄</small> nanocomposites as promising candidates for sustainable and multifunctional biomaterials, offering load-bearing support, efficient hyperthermia for osteosarcoma therapy, and accelerated bone regeneration.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 35","pages":" 10982-11000"},"PeriodicalIF":6.1,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144857217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced therapeutic efficacy of cationic liposome-delivered nerve growth factor antisense oligonucleotide for interstitial cystitis/bladder pain syndrome","authors":"Yuan Gao, Ying Zhao, Lin Zhu, Hanwei Ke, Wenxin Li, Sulaiman Ganiu Bolaji, Huimei Wang, Kexin Xu and Lianyan Wang","doi":"10.1039/D5TB00820D","DOIUrl":"10.1039/D5TB00820D","url":null,"abstract":"<p >The pathogenesis of interstitial cystitis/bladder pain syndrome (IC/BPS) remains unclear, and there is no definitive treatment for this condition. Studies have shown that antisense oligonucleotide (asODN) targeting nerve growth factor (NGF) can downregulate the level of NGF in the bladder, however, the uptake of NGF asODN by the body is limited. Therefore, this study constructed cationic liposomes (CLs) as a delivery system to carry NGF asODN and evaluated its functional efficacy on the bladder. The results indicated that the optimized CLs/asODN delivery system had an average particle size of approximately 200 nm, an average zeta potential of around +53 mV, and an encapsulation efficiency of over 90% with good stability. Additionally, CLs/asODN significantly facilitated the uptake of asODN fluorescence by the urothelium, with an uptake rate of 14.6%, which was 40.2 times free asODN. In a rat model of IC/BPS, treatment with CLs/asODN reduced voiding frequency, significantly increased maximum cystometric capacity, prolonged inter-contraction interval of the bladder, and improved bladder compliance. Furthermore, hematoxylin-eosin staining and immunohistochemical analysis revealed significantly reduced expression levels of NGF, PACAP, Piezo2, CCL2, IL-6, and TGF-β factors after treatment, indicating that the overexpression of NGF in the bladder could be indirectly blocked by complexing NGF asODN with cationic liposomes. The CLs/asODN prepared in this study improved the adhesion and penetration of the drug at the bladder mucosa site, effectively alleviated bladder dysfunction in rats, and further enhanced the inhibitory effect of asODN on NGF, which may provide a new strategy for the treatment of IC/BPS.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 37","pages":" 11663-11673"},"PeriodicalIF":6.1,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d5tb00820d?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the mechanism of synergistic antibacterial effect of silver nanoparticles in combination with vancomycin against Enterococcus species by fluorescence microscopy visualization","authors":"Lucie Válková, Lucie Suchánková Hochvaldová, Martin Mistrík, Milan Kolář, Kateřina Langová, Hana Kolářová, Barbora Štefková, Robert Prucek, Libor Kvítek and Aleš Panáček","doi":"10.1039/D5TB01231G","DOIUrl":"10.1039/D5TB01231G","url":null,"abstract":"<p >Silver nanoparticles (Ag NPs) significantly enhance the antibacterial activity of antibiotics and even restore their effect against resistant strains, making them a promising option for overcoming bacterial resistance to antibiotics. However, the exact mechanism of their synergistic effect with antibiotics at the cellular level has not been elucidated. In this work, we synthesised rhodamine-labelled Ag NPs and described, for the first time, the multi-level non-specific mechanism of the synergistic antibacterial effect of fluorescently labelled Ag NPs and a fluorescent vancomycin conjugate against vancomycin-resistant enterococci using high-resolution fluorescence microscopy. The multi-level mechanism of the synergistic effect of Ag NPs and vancomycin is mainly based on the disruption of the strength and integrity of the cell wall, which becomes unstable, loses strength and subsequently disintegrates due to the oxidative stress caused by Ag NPs and the residual effect of vancomycin. In addition, Ag NPs penetrate the bacterial cell and deform the bacterial DNA, which also significantly increases the synergistic antibacterial effect. This work represents an advance in understanding the mechanism of synergistic effect of Ag NPs with antibiotics against resistant bacteria, an important finding for a potential approach to effectively combat the unsolved problem of increasing resistance of pathogenic bacteria to traditional antibiotics.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 35","pages":" 10903-10915"},"PeriodicalIF":6.1,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d5tb01231g?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144857223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[7]Helicene-backboned quaternary ammonium salts: synthesis, photophysical investigation, and lysosomal tracking","authors":"Deepshikha, Sanjay S. Bisht, Nand Lal, Puja Singh, Jonaid A. Malik, Javed N. Agrewala and Aslam C. Shaikh","doi":"10.1039/D5TB01192B","DOIUrl":"10.1039/D5TB01192B","url":null,"abstract":"<p >Polyaromatic quaternary ammonium salts (PQASs) are of significant interest due to their promising applications in biological and materials sciences. The incorporation of a heteroatom significantly modifies the electronic and chemical properties of these molecules, influencing their absorption and emission characteristics, as well as the HOMO–LUMO gap. Herein, we report the synthesis and photophysical investigation of [7]helicene-backboned quaternary ammonium salts. These compounds exhibit excellent stability, absorb light in the visible region with <em>λ</em><small>_abs</small> ranging from 385 to 395 nm, and show emission in the green wavelength spectrum at <em>λ</em><small>_em</small> between 541 and 552 nm. Also, they are redox-active and feature a structurally defined double helical axis. Single-crystal X-ray diffractometry has demonstrated the presence of a double helical structure within the crystal packing, which is characterized by the linkage of M–P and P–M heterodimers. Additionally, they show photoluminescence capability (<em>ϕ</em><small>_f</small>) of up to 0.57, with fluorescence lifetimes in the range of 1.81–3.17 ns. Notably, these fluorophores turned out to be potential cell imaging agents. Colocalization studies that utilized LysoTracker Red probes as standard lysosomal trackers demonstrated that the [7]helicene QAS probe is efficacious in specifically labeling lysosomes in the neuroblastoma (N2a) cell line and RAW 264.7 macrophage cells. Additionally, to elucidate their electronic profiles, we employed time-dependent density functional theory calculations.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 38","pages":" 12146-12153"},"PeriodicalIF":6.1,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}