Journal of Materials Chemistry B最新文献

{"title":"Competitive electrochemical assay of CA19-9 on gold nanoparticles/porous carbons nanohybrid amplified by polyoxometalate/aptamer/graphene quantum dot-decorated gold nanotags","authors":"Sopit Phetsang, Cécile Jouanne, Mohamed Mallouki, Thuan-Nguyen Pham-Truong, Sébastien Peralta, Cédric Vancaeyzeele, Philippe Banet, Pierre-Henri Aubert, Jaroon Jakmunee and Kontad Ounnunkad","doi":"10.1039/D5TB01565K","DOIUrl":"10.1039/D5TB01565K","url":null,"abstract":"<p >Although recent breakthroughs have brought hope for a complete cure for cancer, early and rapid diagnosis remains crucial for effective treatment. Carbohydrate antigen 19-9 (CA19-9) is a clinically established biomarker widely used in the diagnosis and management of gastric and pancreatic cancers. Accurate detection of CA19-9 is crucial for early diagnosis, treatment monitoring, and predicting recurrence. This study presents a novel competitive electrochemical aptasensor for CA19-9 detection, utilizing a composite of highly porous carbons (carboHIPEs) derived from the pyrolysis of polyHIPEs and polyethylenimine-capped gold nanoparticles (PEI-AuNPs) on a modified screen-printed electrode (SPE). The high porosity of carboHIPEs and the functionalized AuNPs enhance protein immobilization, boosting assay sensitivity and specificity. In this indirectly competitive assay, the CA19-9 is immobilized on the electrode surface, followed by binding with CA19-9-specific aptamer/polyoxometalate (POM)/graphene quantum dots (GQDs)@citrate-capped gold nanoparticles (CT-AuNPs) for signal amplification. The redox-active POM signal is measured <em>via</em> differential pulse voltammetry (DPV) in 0.20 M acetate buffer, with the electrochemical signal inversely correlating to CA19-9 concentration. The linear detection range of the sensor is observed from 0.10 to 200 U mL<small>⁻¹</small> with a limit of detection (LOD) of 0.039 U mL<small>⁻¹</small>, making it suitable for the clinical diagnosis of both cancers. Integrating nanomaterials such as carboHIPEs, GQDs, and CT-/PEI-AuNPs yields high overall conductivity, increased electroactive surface area, and excellent biocompatibility, resulting in a high electrochemical response. This sensing platform offers broad detection capabilities and high selectivity, positioning it as a promising tool for detecting cancer biomarkers.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 37","pages":" 11652-11662"},"PeriodicalIF":6.1,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A zwitterionic dual-network hydrogel patch with anti-inflammatory properties for efficient peritoneal adhesion prevention","authors":"Yangrui Du, Li Ma, Shiying Lang, Shan Liu, Kang Dai, Kaijun Li, Qian Wang and Gongyan Liu","doi":"10.1039/D5TB01786F","DOIUrl":"10.1039/D5TB01786F","url":null,"abstract":"<p >Postoperative peritoneal adhesion, driven by inflammatory response and fibrotic deposition, remains the most common complication following abdominal surgeries, with limited effective solutions. Herein, a dual-network hydrogel patch (GPSB) is developed for effective peritoneal adhesion prevention through interpenetrating a gelatin network with a zwitterionic polysulfobetaine (PSB) network. The biodegradable gelatin network is dynamically crosslinked <em>via</em> zinc ion (Zn<small>²⁺</small>)–polyphenol coordination, endowing the patch with inherent antibacterial and pro-healing activities. Through <em>in situ</em> polymerization, a crosslinked PSB network is interwoven with the gelatin matrix, markedly increasing the Young's modulus of hydrogels to 97.7 ± 5.2 kPa and greatly enhancing their mechanical integrity. Moreover, the resulting GPSB hydrogel achieves a shear strength of 28.5 kPa, on par with commercial cyanoacrylate-based bio-glues. Moreover, the highly biocompatible PSB component effectively resists nonspecific protein adsorption by 46-fold and modulates macrophage-mediated inflammatory responses, reducing the secretion of inflammatory cytokines by approximately 70%. Through the synergistic antibacterial and anti-inflammatory functions, the GPSB hydrogel patch provides robust protection against postoperative inflammation and achieves efficient prevention of peritoneal adhesions. This study highlights an effective and multifunctional hydrogel patch for next-generation anti-adhesion biomaterials.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 38","pages":" 12111-12123"},"PeriodicalIF":6.1,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145002209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colorimetric point-of-care diagnostic to monitor Enterococcus faecalis causing urinary tract infection","authors":"Maria M. Morim, Patrick Rupper, Stefanie Altenried, Adrian Meier, René M. Rossi, Qun Ren, Luciano F. Boesel and Giorgia Giovannini","doi":"10.1039/D5TB01437A","DOIUrl":"10.1039/D5TB01437A","url":null,"abstract":"<p >Urinary tract infections (UTIs) are among the most common bacterial infections, affecting approximately 150 million people worldwide each year. Currently, diagnosis is often made using culture-based methods, which are time-consuming and therefore costly. Point-of-care (POC) devices have the potential to provide a rapid and accurate UTI diagnosis, thereby improving treatment efficacy. In this work, we developed a fast, specific, and accurate colorimetric sensor capable of indirectly detecting <em>Enterococcus faecalis</em> in urine samples by targeting its metabolite, <small>L</small>-lactate. The sensing probe consists of silica nanoparticles (SNP) loaded with MnO<small>₂</small> (MnSNP), functionalised on the surface with the enzyme lactate oxidase (Lac@MnSNP). The sensor enables both qualitative analysis – through a visible colour change – and quantitative analysis, using spectroscopy. The morphology and composition of the probe were characterised at each synthesis step, confirming that the incorporation of MnO<small>₂</small> into SNP and subsequent enzyme functionalisation did not alter nanoparticle morphology. Lac@MnSNP demonstrated responsiveness to <small>L</small>-lactate, showing a linear decrease in signal up to 50 μM of the analyte, with a limit of detection of 31 μM. The probe successfully detected <em>E. faecalis</em> in artificial urine medium and in complex samples at concentrations as low as 10<small>³</small> CFU mL<small>⁻¹</small> within 5 h. These results demonstrate the potential of this probe for fast, accurate, and lactate-specific diagnoses.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 37","pages":" 11640-11651"},"PeriodicalIF":6.1,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d5tb01437a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered hollow Cu2O@ZnO p–n heterojunction nanocomposites for synergistic photocatalytic disinfection and tissue regeneration","authors":"Yu Fen, Miao Xinxin, Xiao Yang, Deng Jianjian, Deng Dan, Cheng Xinyan, Ding Rui and Wang Xiaolei","doi":"10.1039/D5TB01635E","DOIUrl":"10.1039/D5TB01635E","url":null,"abstract":"<p >Photocatalytic antibacterial therapy is a promising method for wound disinfection and treatment. However, the weak photocatalytic antibacterial activity of ZnO stimulated by visible light limits its applications. In this study, porous Cu<small>₂</small>O@ZnO heterojunctions with enhanced visible light response are successfully synthesized by coupling ZnO and Cu<small>₂</small>O using a one-pot water bath method. These synthesized Cu<small>₂</small>O@ZnO heterojunctions can be used to treat bacterial infection under yellow light irradiation with a high efficacy of 99.99% due to the enhanced photocatalytic activity. This is mainly due to the p–n heterojunction in Cu<small>₂</small>O@ZnO, which can accelerate charge transfer and effectively suppress the photogenerated electron–hole pair recombination. Animal experiment results demonstrate that Cu<small>₂</small>O@ZnO not only exhibits excellent antibacterial activity upon yellow light irradiation but also facilitates angiogenesis and inhibits inflammation, resulting in successful infectious wound regeneration in mice. Therefore, this heterojunction material can serve as a promising platform for effective healing of bacteria-infected wounds.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 38","pages":" 12246-12256"},"PeriodicalIF":6.1,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144994931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soft nanoparticles incorporating a benzo[c]xanthene fluorophore: facile synthesis and ratiometric pH sensing","authors":"Laura McKay, Natali Joma, Dusica Maysinger and Ashok Kakkar","doi":"10.1039/D5TB01322D","DOIUrl":"10.1039/D5TB01322D","url":null,"abstract":"<p >Polymeric nanoparticles offer considerable potential in resolving key issues related to the delivery of small lipophilic pharmaceutics. However, significant challenges remain with respect to the development and application of multifunctional, easily accessible fluorescent tools for tracing the cellular uptake and trafficking of soft nanoparticles. We have evaluated a platform for this purpose utilizing the pH-responsive molecular sensor seminaphthofluorescein-C (benzo[<em>c</em>]xanthene ‘SNAFL-C’); its chemical conjugation to an asymmetric miktoarm star polymer (AB<small>₂</small>) at its hydrophobic arm terminus; and traceable polymeric nanoparticles through self-assembly of SNAFL-AB<small>₂</small> or physical encapsulation of the fluorophore. The characteristic high Stokes shift and ratiometric emission behaviour of SNAFL-C were retained in its polymer conjugate as well as in the self-assembled structures. Assemblies of the miktoarm fluorophore conjugate exhibited moderately reduced brightness as a result of aggregation induced quenching, an effect not observed for the physically encapsulated species. Fluorescence quenching experiments probing the partitioning of SNAFL-C in the core shell structures revealed localization of the primary fluorescent species in equilibrium based on their physiochemical properties, providing rationalization of differing aggregation behaviour observed for physically encapsulated and covalently linked formulations. Live cell fluorescence imaging in human glioblastoma cells integrating both SNAFL-C and its nanoformulations demonstrated the utility of the fluorophore in biological imaging applications and highlighted the necessity of detailed and stepwise spectral and photophysical evaluations. Collectively, these systems offer new avenues to explore fluorescence imaging using polymeric nanocarriers, leading to insights of broad importance to drug delivery and theragnostics.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 37","pages":" 11790-11808"},"PeriodicalIF":6.1,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d5tb01322d?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular engineering based on four-arm perylene diimide chromophores toward hypoxia-induced specific photothermal therapy","authors":"Kecheng Huang, Xiangkun Cui, Miaomiao Zhen, Xueying Zhang, Fei Yang, Wei Wei and Yanqing Xu","doi":"10.1039/D5TB01583A","DOIUrl":"10.1039/D5TB01583A","url":null,"abstract":"<p >Perylene diimide (PDI) radical anions have attracted increasing attention as hypoxia-responsive photothermal agents due to their strong near-infrared (NIR) absorption and efficient photothermal conversion. However, their biomedical application is often limited by aggregation-induced quenching and poor structural tunability. In this work, we report a rationally engineered four-arm PDI derivative (PDI-4Alky·4Cl) bearing terminal alkyne groups, which not only suppresses π–π stacking <em>via</em> steric and electrostatic repulsion, but also serves as a versatile molecular scaffold for further functionalization. The resulting PEGylated product (PDI-2PEG) exhibits excellent aqueous stability, high radical yield, and strong NIR photothermal performance (conversion efficiency up to 51.6%). Importantly, PDI-2PEG demonstrates hypoxia-triggered radical generation and tumor-specific photothermal ablation <em>in vivo</em>. This study highlights a structurally modular PDI platform that integrates radical-based photothermal therapy with synthetic flexibility, offering a promising strategy for the development of next-generation tumor-selective theranostic agents.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 38","pages":" 12124-12133"},"PeriodicalIF":6.1,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Au@mSiO2 nanocomposites with large pores for protein transport","authors":"Andrea Montero-Oleas, Yoann Roupioz, Philippe Trens, Stéphanie Kodjikian, Silvio J. Ludueña, Lía I. Pietrasanta, Sara A. Bilmes and Xavier Cattoën","doi":"10.1039/D5TB01290B","DOIUrl":"10.1039/D5TB01290B","url":null,"abstract":"<p >Gold-mesoporous silica (Au@mSiO<small>₂</small>) core–shell nanoparticles (NPs) have shown interesting potential for the loading of molecules to be delivered by plasmonic heating. In this study, we describe the unprecedented synthesis of Au@mSiO<small>₂</small> NPs with large pores (lp-Au@mSiO<small>₂</small>), aiming at encapsulating large biomolecules such as proteins. Starting from recently reported Au@mSiO<small>₂</small> seeds with a diameter of 45 nm, two strategies are presented to grow a mesoporous shell with large pores. The most interesting NPs (lp-Au@mSiO<small>₂</small>) were obtained with the biphasic stratification approach, yielding NPs with large conical pores (10–20 nm openings), characterized in depth by N<small>₂</small>-sorption and electron microscopies. Atomic force microscopy (AFM) with a sharp tip was used for the first time with these mesoporous materials to probe the accessibility of the pore openings. Biphasic stratification provides NPs with good colloidal and hydrolytic stabilities in aqueous saline medium (PBS) allowing the incubation of these NPs with two model proteins: horse radish peroxidase (HRP) and red fluorescent protein (RFP). lp-Au@mSiO<small>₂</small> exhibit a significantly larger loading capacity with respect to NPs with similar diameter, either non-porous or with narrower pores, providing evidence that the proteins can indeed be encapsulated within the pores.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 36","pages":" 11342-11352"},"PeriodicalIF":6.1,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d5tb01290b?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal and spatial control over fiber alignment within hyaluronic acid hydrogels using magnetic fields","authors":"Grace Schwarz and Julianne L. Holloway","doi":"10.1039/D5TB00694E","DOIUrl":"10.1039/D5TB00694E","url":null,"abstract":"<p >Wound healing is tightly regulated in both space and time. To mimic this behavior, we designed magnetically-responsive, fiber–hydrogel composites. When covalently crosslinked hydrogels were combined with layer-by-layer stacking, we demonstrated precise spatial control over fiber orientation. For temporal control, we used non-covalently crosslinked hydrogels to enable <em>in situ</em> fiber alignment.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 36","pages":" 11201-11209"},"PeriodicalIF":6.1,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A hyperbranched polysiloxane with imine bonds: red fluorescence emission with a long fluorescence lifetime at room temperature","authors":"Qi Li, Yujie Qiao, Zheng Li, Rui Wu, Yekun Zhang, Yan Zhao, Wei Tian, Rumin Wang, Weixu Feng and Hongxia Yan","doi":"10.1039/D5TB01335F","DOIUrl":"10.1039/D5TB01335F","url":null,"abstract":"<p >In recent years, due to their excellent biocompatibility and environmental friendliness, non-conjugated AIE polymers have attracted extensive attention. However, problems such as short emission wavelengths and low fluorescence lifetimes have severely limited the applications of these materials. In this paper, a siloxane monomer A1 containing an imine bond was designed and synthesized, and it was used as a raw material to synthesize a hyperbranched polysiloxane P1 with imine bonds and hydroxyl groups at the end of the polymer chain. Surprisingly, P1 exhibited bright red fluorescence, and its fluorescence lifetime reached an uncommon microsecond level (11.40 μs). The luminescence mechanisms of A1 and P1 were explored through experimental and theoretical calculations. It was proposed that the introduction of local rigid planar structures could promote charge delocalization and enhance through-space interactions. Thus, the emission wavelength has a red-shift and the fluorescence lifetime has been improved. In addition, the solvent effects and pH responsiveness of P1, as well as the application of P1 for Mn<small>²⁺</small> detection, were also studied.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 37","pages":" 11687-11695"},"PeriodicalIF":6.1,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PET-leveraged ALDH probe toward cancer stem cells","authors":"Ji Hyeon Kim, Bryan Yat Kit Hui, Jieun Lee, Kyeong-woo Lee, Jungryun Kim, Yujin Kim, Ming Hui Chua, Jianwei Xu, Min-Goo Lee, Xiaogang Liu and Jong Seung Kim","doi":"10.1039/D5TB01253H","DOIUrl":"10.1039/D5TB01253H","url":null,"abstract":"<p >Developing novel aldehyde dehydrogenase (ALDH) probes is essential for enhancing the localization and visualization of cancer stem cells (CSCs) by improving specificity and signal-to-noise ratios. This study introduces four new ALDH fluorescent probes (<strong>AldeCou1–4</strong>), designed using a coumarin-linker-benzaldehyde scaffold. Among them, <strong>AldeCou-1</strong> exhibits a significant Stokes shift of 125 nm (<em>λ</em><small>_ex</small>/<em>λ</em><small>_em</small> = 380/505 nm), which enhances the signal-to-noise ratio and minimizes inner-filter effects. <strong>AldeCou-1</strong> demonstrated superior green fluorescence imaging in A549 and MDA-MB-231 cells, enabling differentiation between these cell lines based on ALDH activity. Compared to conventional ALDH activity assay kits, such as ALDEFLUOR, which require additional buffers and inhibitors that complicate imaging protocols, <strong>AldeCou-1</strong> enables simplified assay conditions by eliminating the need for ATP-binding cassette (ABC) transporter inhibitors and specialized buffers. Computational analysis suggests that the fluorescence turn-on mechanism of <strong>AldeCou-1</strong> is driven by ALDH-mediated photoinduced electron transfer (PET). These findings provide valuable mechanistic insights and pave the way for developing next-generation ALDH probes for improved CSC screening and characterization.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 37","pages":" 11809-11820"},"PeriodicalIF":6.1,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}