Journal of Materials Chemistry B最新文献

{"title":"Bioinspired silk protein modification to develop instant dissolvable microneedles with superior mechanical properties and long-term biomolecule stabilization†","authors":"Jayakumar Rajendran, Jeyashree K., Sujith M. S., Lalitha Devi Alluri and Jyotsnendu Giri","doi":"10.1039/D4TB02836H","DOIUrl":"10.1039/D4TB02836H","url":null,"abstract":"<p >Dissolvable microneedles (DMNs) obtained from silk proteins have been considered most promising due to the biocompatibility, tuneable mechanical properties, and superior biomolecule stabilization properties of their silk matrix, required for cold chain-free storage and transport of therapeutic biomolecules and vaccines. However, despite their excellent potential, silk-based microneedles with instant dissolvability, superior mechanical properties, and storage stability have not yet been reported. Reported DMNs prepared with &lt;5% silk concentration without β-sheets show poor mechanical and storage stability. Conversely, silk MNs prepared using &lt;5% silk treated with an organic solvent or &gt;5% silk may have sufficient mechanical properties but lose their instant dissolubility due to β-sheet formation during solvent treatment and storage, respectively. Thus, herein, we address these challenges for the first time <em>via</em> the biomimetic modification of silk proteins to mimic the molecular structure of human serum albumin (HSA) and silk protein molecules in the silk gland lumen of silkworms, resulting in high solubility and low viscosity. Our biomimetic modified silk (MS) allowed us to prepare DMNs in higher concentrations (&gt;10% w/v up to 20% w/v) with a stabilizing agent (&gt;10% w/v), exhibiting superior mechanical properties of &gt;45 N and instant dissolvability even after 6 months of storage at RT without inducing β-sheet formation. Furthermore, MS-DMN facilitated the exceptional storage stability of platelet-rich plasma (PRP) with &gt;80% retention for six months when stored at 4 °C or 25 °C and &gt;90% at 40 °C at 75% RH for one month, as confirmed through <em>in vitro</em> cell proliferation assay, in ova (CAM assay), and <em>in vivo</em> diabetic wound studies. Thus, our novel biomimetic MS-DMN exhibits superior mechanical properties and exceptional biomolecule storage stability, enabling potential cold chain-free preservation and transportation for various biomedical applications.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 24","pages":" 7072-7089"},"PeriodicalIF":6.1,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d4tb02836h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144103264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The combined application of exosomes/exosome-based drug preparations and ultrasound","authors":"Xiuli Wen and Yi Hao","doi":"10.1039/D4TB01530D","DOIUrl":"10.1039/D4TB01530D","url":null,"abstract":"<p >Exosomes are small extracellular vesicles with a diameter of 30–150 nm, secreted by a variety of cells and containing various active substances such as nucleic acids, proteins and lipids. The use of exosomes as drug carriers for targeted delivery of therapeutics has been studied for a long time. Ultrasound is recognized as a non-invasive diagnostic and therapeutic method for assisting drug loading and targeted delivery, cellular uptake and therapy. In this review, we summarize the applications of ultrasound in assisting drug loading into exosomes, targeted delivery of exosome-based drug formulations, cellular uptake, and therapy, and explore the prospects for the combined application of exosomes/exosome-based drug formulations and ultrasound.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 23","pages":" 6626-6637"},"PeriodicalIF":6.1,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d4tb01530d?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144103270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel yellow light-responsive SnO2/SnS2 piezo-photocatalysts with excellent performances for tooth whitening and biofilm eradication†","authors":"Jiahe Song, Shiping Yuan, Shujuan Liu, Yunsong Wang, Bocan Yang, Linwan Ji, Liangcan He and Shaoqin Liu","doi":"10.1039/D5TB00407A","DOIUrl":"10.1039/D5TB00407A","url":null,"abstract":"<p >Oral problems caused by pathogens and tooth discoloration have posed great threats to public health in recent years. Timely killing of cariogenic bacteria and removing surface pigments are the key points to treat yellow teeth to restore healthy whitening. Piezo-photocatalysis has been proved to be an effective strategy for treating yellow teeth. However, few effective and safe nanomaterials have been developed to address this issue in the oral field. Herein, yellow light-responsive SnO<small>₂</small>/SnS<small>₂</small> heterostructures are proposed for piezo-photocatalytic biofilm eradication and tooth whitening for the first time. Initially, XRD and HRTEM results experimentally verified the formation of SnO<small>₂</small>/SnS<small>₂</small> heterostructures. Further, UV-vis DRS spectra indicated that the absorbance in the visible region was effectively improved after the formation of SnO<small>₂</small>/SnS<small>₂</small> heterostructures. Subsequently, yellow light with excellent biocompatibility was combined with ultrasonic treatment to explore the piezo-photocatalytic performances of SnO<small>₂</small>/SnS<small>₂</small> for tooth whitening and biofilm removal. Results demonstrated that the SnO<small>₂</small>/SnS<small>₂</small> heterostructure prepared with a TAA : SnO<small>₂</small> molar ratio of 1 : 1 for 3 h exhibited the best piezo-photocatalytic performance. The degradation efficiency for the food colorant indigo carmine reached 94.12%, which was much higher than that of single SnS<small>₂</small> (48.31%), single SnO<small>₂</small> (near zero) and treating with only irradiation (63.03%) and only ultrasonic (79.41%). Simultaneously, the heterostructures exhibited excellent piezo-photocatalytic tooth whitening effect on stained teeth. Moreover, the SnO<small>₂</small>/SnS<small>₂</small> heterojunctions exhibited excellent piezo-photocatalytic performances in bacteria killing and biofilm removal, and the antibacterial efficiencies reached 77.3% and 56.5% for planktonic <em>S. mutans</em> and biofilms, respectively. In addition, synergistic treating process of SnO<small>₂</small>/SnS<small>₂</small> heterostructures resulted in excellent biocompatibility, including much less cytotoxicity and negligible enamel damage. In-depth mechanism investigation indicated that the improved piezo-photocatalytic performances were due to the increased carrier separation efficiency and ROS productivity of SnO<small>₂</small>/SnS<small>₂</small> heterostructures, demonstrating the great potential of SnO<small>₂</small>/SnS<small>₂</small> heterostructures for future dental care field.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 24","pages":" 7181-7195"},"PeriodicalIF":6.1,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of polyacrylic acid-coated AuPd@FexOy nanoparticles for synergistic chemodynamic and photothermal therapy of osteosarcoma†","authors":"Zeping Liu, Haoyu Sun, Yongjie Shan, Haodong Wang, Wenqi Luo, Rui Gu and Zhenxin Wang","doi":"10.1039/D5TB00364D","DOIUrl":"10.1039/D5TB00364D","url":null,"abstract":"<p >The treatment outcomes of osteosarcoma (OS) can be improved significantly by multimodal synergistic therapy. In this report, polyacrylic acid-coated AuPd@Fe<small>_<em>x</em></small>O<small>_<em>y</em></small> core@shell nanoparticles (termed as PAA-AuPd@Fe<small>_<em>x</em></small>O<small>_<em>y</em></small> NPs) have been synthesized for magnetic resonance imaging (MRI)-guided synergistic chemodynamic therapy and photothermal therapy (CDT–PTT) of OS through a simple two-step process, which includes synthesis of AuPd@Fe<small>_<em>x</em></small>O<small>_<em>y</em></small> NPs by a self-assembly redox strategy, and coating PAA on the AuPd@Fe<small>_<em>x</em></small>O<small>_<em>y</em></small> NP surface by physical adsorption. The amorphous Fe<small>_<em>x</em></small>O<small>_<em>y</em></small> shell of the PAA-AuPd@Fe<small>_<em>x</em></small>O<small>_<em>y</em></small> NPs not only exhibits a pH-responsive <em>T</em><small>₁</small>-weighted MRI contrast ability, but also can generate a large number of hydroxyl radicals (˙OH) by the glutathione (GSH) amplified Fenton reaction. Furthermore, the production of reactive oxygen species (ROS) can be enhanced by 808 nm near infrared (NIR) laser irradiation because the PAA-AuPd@Fe<small>_<em>x</em></small>O<small>_<em>y</em></small> NPs exhibit excellent photothermal conversion capability. The features of PAA-AuPd@Fe<small>_<em>x</em></small>O<small>_<em>y</em></small> NPs improve the tumour-MRI sensitivity, and facilitate synergistic CDT–PTT of tumours. The growth of MG63 OS in a mouse model is effectively suppressed by the PAA-AuPd@Fe<small>_<em>x</em></small>O<small>_<em>y</em></small> NPs with 808 nm NIR laser irradiation.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 22","pages":" 6551-6559"},"PeriodicalIF":6.1,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144096463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ab initio study of precipitate stability in an Mg–Zn–Zr–Y alloy and its effects on corrosion, antimicrobial and in vitro biocompatibility","authors":"Satyabrata Nigamananda Sahoo, Indu Avula, Santanu Mandal, Surasree Pal, Vamsi Krishna Balla and Mangal Roy","doi":"10.1039/D5TB00488H","DOIUrl":"10.1039/D5TB00488H","url":null,"abstract":"<p >Magnesium (Mg) alloy has long been projected as a degradable biomaterial with a special focus on its corrosion behaviour, mechanical properties and biocompatibility. In this study, we developed an Mg–5Zn–0.5Zr–0.9Y alloy and studied the effects of Mg<small>₇</small>Zn<small>₃</small> and Mg<small>₂₄</small>Y<small>₅</small> precipitates on its mechanical, corrosion, antimicrobial, and <em>in vitro</em> biocompatibility properties. The cast specimen exhibited a continuous network of Mg<small>₇</small>Zn<small>₃</small> precipitates and a weaker basal texture, which adversely affected the mechanical and corrosion properties. The application of thermomechanical treatment preferentially dissolutes the Mg<small>₇</small>Zn<small>₃</small> precipitate network compared to the Mg<small>₂₄</small>Y<small>₅</small> precipitate owing to its lower cohesive energy, lower enthalpy of formation and lower bonding electrons, as calculated using the first principle study. This resulted in a significant reduction in the volta potential difference between the precipitates and the matrix, which simultaneously improved corrosion resistance and ductility. Moreover, the increased area fraction of Mg<small>₂₄</small>Y<small>₅</small> fine precipitates in the forged specimen contributed to its enhanced corrosion resistance. DFT calculations indicated a lower total density of state (DOS) value near the Fermi energy level of Mg<small>₂₄</small>Y<small>₅</small>, which retarded electron loss capability and thereby reduced the corrosion rate. The higher corrosion resistance of the forged sample was due to lower defect density, as determined through Mott–Schottky analysis. All samples exhibited excellent antimicrobial properties against Gram-negative <em>E. coli</em> bacteria. Improved degradation properties of the forged specimen enhanced cell viability and alkaline phosphatase activity against MC3T3-E1 cells, indicating its non-toxic nature.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 22","pages":" 6533-6550"},"PeriodicalIF":6.1,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144096409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermo-responsive hydrogel via sustained Co-delivery of TA and PDGF to modulate the diabetic microenvironment and accelerate diabetic wound healing†","authors":"Jisun Kim, Ki Wan Bong, Jung-Kyo Cho and Soo-Chang Song","doi":"10.1039/D5TB00563A","DOIUrl":"10.1039/D5TB00563A","url":null,"abstract":"<p >In modern society, the need for diabetic wound healing is increasing due to the increase in the number of diabetic patients. In particular, chronic inflammation is a major problem in diabetic wounds due to excessive accumulation of reactive oxygen species (ROS). Therefore, it is essential to remove ROS and promote angiogenesis for effective diabetic wound healing. In this study, we developed a thermo-responsive poly(organophosphazene) hydrogel system (TSP–TP) designed to deliver antioxidants and growth factors for a long period of time. The TSP–TP hydrogel stably loads and continuously releases tannic acid (TA) and platelet-derived growth factor (PDGF) through various physical interactions. Effective ROS scavenging induced macrophage polarization and alleviated chronic inflammation, while the sustained release of PDGF promoted angiogenesis, ultimately maximizing wound healing efficacy in a diabetic mouse model. Based on these results, the proposed TSP–TP hydrogel demonstrates synergistic effects through sustained delivery of antioxidants and growth factors, demonstrating a promising system with high applicability in diabetic wound treatment.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 24","pages":" 7090-7105"},"PeriodicalIF":6.1,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d5tb00563a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A 3D-bioprinted dermal-like scaffold incorporating fibroblasts and DRG neurons to investigate peripheral nerve regeneration†","authors":"Francesco Formaggio, Emanuela Saracino, Marianna Barbalinardo, Eva Clemente, Franco Corticelli, Sara Buoso and Simone Bonetti","doi":"10.1039/D4TB02823F","DOIUrl":"10.1039/D4TB02823F","url":null,"abstract":"<p >Peripheral nervous system (PNS) regeneration is a rapidly advancing field with critical implications for addressing sensory impairments and neuropathic conditions. Dorsal root ganglion (DRG) neurons, essential for sensory transmission, exhibit regenerative potential through axonal regeneration. However, the mechanisms driving these processes are not yet understood. This study introduces an innovative 3D-bioprinted fibroblasts/DRG co-culture construct, specifically designed to investigate and characterize PNS regeneration and wiring mechanisms under both physiological and pathophysiological conditions. By characterizing bioink rheology and optimizing bioprinting parameters, we created a stable, biocompatible derma-like construct supporting cell adhesion and growth. Bioprinted 3T3 fibroblasts demonstrate high viability and proliferation, while DRG neurons exhibit enhanced neurite outgrowth and complex branching patterns within the co-culture system. These findings highlight the role of fibroblasts in promoting axonal regeneration and provide a robust <em>in vitro</em> platform for studying sensory system reinnervation. This model lays the foundation for developing personalized therapies for neuropathic pain and sensory dysfunction, advancing both fundamental neuroscience and translational medicine.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 24","pages":" 7034-7047"},"PeriodicalIF":6.1,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d4tb02823f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144153105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered Ti3C2(O,Cl) MXenes with dual functionalization: a new Frontier in targeted head and neck squamous cell carcinoma and breast adenocarcinoma†","authors":"Jhansi Chintakindi, Ganesh Panditrao Lahane, Arti Dhar and Afkham Mir","doi":"10.1039/D5TB00302D","DOIUrl":"10.1039/D5TB00302D","url":null,"abstract":"<p >Ti<small>₃</small>C<small>₂</small>T<small>_<em>x</em></small> MXenes have attracted significant attention in the realm of anticancer therapeutics owing to their remarkable properties, including cyto-compatibility and targeted drug delivery capabilities. In this study, Ti<small>₃</small>C<small>₂</small> was intentionally modified with both chlorine and oxygen surface groups, as each of these functional groups have individually demonstrated promising anticancer properties. Our aim was to combine them in a single compound to explore how this dual-functionalized material might perform in a therapeutic context. This study synthesizes Ti<small>₃</small>C<small>₂</small>(O,Cl) MXenes using a novel electrochemical etching technique that allows for precise tailoring of the surface terminations with O and Cl groups. The synthesised Ti<small>₃</small>C<small>₂</small>(O,Cl) has biological activity in two cancerous (FaDu and MCF-7) and two normal (H9C2 and HEK-293) cell lines. The results of cytotoxicity data showed that the observed toxic effects were higher against cancerous cells (∼91%) than normal cells (∼40%). The mechanisms of potential toxicity were also elucidated. The synthesized Ti<small>₃</small>C<small>₂</small>(O,Cl) MXene has an effect on oxidative stress, resulting in an increase of more than 91.44% in reactive oxygen species (ROS) production in malignant cells. The results of this study provide major insights to date into the biological activity of Ti<small>₃</small>C<small>₂</small>(O,Cl) MXenes and develop their application in anticancer treatments.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 22","pages":" 6329-6341"},"PeriodicalIF":6.1,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/tb/d5tb00302d?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pioneering bactericidal efficacy with nitrogen doping and zinc oxide nanoparticle decoration on carbon nanosheets†","authors":"Omnarayan Agrawal, Bani Preet Kaur, Radhika Chaurasia, Hitesh Kumar Sharma, Geetika Jain, Madhav Krishn Goswami, Sandip Chakrabarti and Monalisa Mukherjee","doi":"10.1039/D5TB00516G","DOIUrl":"10.1039/D5TB00516G","url":null,"abstract":"<p >The escalating prevalence of drug-resistant pathogens poses a significant threat to global health, contributing to elevated mortality rates and inflated healthcare expenses. To combat antibacterial resistance, carbon-based nanocomposites incorporating metal oxides have emerged as a promising solution in the development of advanced antibacterial agents. In this quest, we propose a nascent strategy to synthesize zinc oxide-decorated carbon nanosheets (ZnO@CNSn) <em>via</em> a co-precipitation method. The crystalline ZnO nanoparticles (ZnO-NPs) are homogeneously dispersed throughout a framework of melamine-enriched carbon nanosheets (CNSn). The presence of pyrrolic-N and pyridinic-N functionalities in ZnO@CNSn enhances the charge transfer kinetics and creates nucleation sites for uniform dispersion of ZnO-NPs, mitigating particle aggregation. Remarkably, XPS analysis reveals a distinct shift in peak intensity, characterized by a reduction in pyrrolic-N and a corresponding increase in pyridinic-N. This conversion of pyrrolic-N to pyridinic-N due to incorporation of ZnO-NPs onto CNSn plays a crucial role in improving its bactericidal effect. The antibacterial assays against Gram negative <em>Escherichia coli</em>, Gram positive <em>Staphylococcus aureus</em> and methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) confirm the bactericidal activity of ZnO@CNSn. Additionally, the SEM micrographs show altered bacterial morphology on interaction with the nanocomposites, further validating the effective bactericidal properties. Moreover, ZnO@CNSn exhibits enhanced cytocompatibility compared to CNSn. These findings underscore the promising potential of the ZnO-decorated CNSn architecture as a robust platform for advanced antibacterial applications.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 22","pages":" 6519-6532"},"PeriodicalIF":6.1,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A platinum(iv)–crocetin nanoplatform with intracellular morphological transformation for enhanced colorectal cancer therapy†","authors":"Xue Rui, Zhetong Jin, Lu Li, Jia Liu, Jiarong Mao, Xuejiao Leng, Hongzhi Qiao, Wei Li, Lingchong Wang, Yugen Chen and Jingjing Wang","doi":"10.1039/D5TB00127G","DOIUrl":"10.1039/D5TB00127G","url":null,"abstract":"<p >Colorectal cancer (CRC) is the third most commonly diagnosed malignancy worldwide. Platinum(<small>II</small>)-based drugs, a cornerstone in CRC treatment, are often limited by significant side effects and suboptimal efficacy. Herein, we present a platinum(<small>IV</small>) prodrug nanoplatform (Pt(<small>IV</small>)–Cro NPs) designed to overcome these challenges through intracellular morphological transformation, enhancing therapeutic outcomes against CRC. Pt(<small>IV</small>)–Cro NPs are formed <em>via</em> the self-assembly of Pt(<small>IV</small>)–crocetin (Pt(<small>IV</small>)–Cro) and mPEG–crocetin (mPEG–Cro), driven by hydrophilic–hydrophobic interactions. These nanoparticles exhibit concentration-dependent morphology, transitioning from rod-shaped structures at lower concentrations to spherical forms at higher concentrations. Notably, Pt(<small>IV</small>)–Cro NPs undergo time-dependent morphological changes within cells. Upon uptake by CT26 cells, the nanoparticles retain a nanorod shape during the first hour but transform into spherical structures within 3 h. These morphological transitions contribute to a remarkable 141-fold reduction in the half-inhibitory concentration (IC<small>₅₀</small>) against CT26 cells compared to cisplatin alone. Pt(<small>IV</small>)–Cro NPs induced 3.14-fold greater apoptosis, 51.2% mitochondrial depolarization, and 55.9% ROS elevation compared to cisplatin. <em>In vivo</em> studies in CT26 tumor-bearing mice reveal that Pt(<small>IV</small>)–Cro NPs significantly outperform cisplatin alone, reducing tumor growth by up to 8.08 times relative to controls. This innovative nanoplatform combines enhanced efficacy with minimized side effects, offering a transformative approach to CRC therapy. The concentration-responsive self-assembly of Pt(<small>IV</small>)–Cro NPs and the occurrence of morphologic transformations within the cell characterize a major advancement in clinical CRC therapeutic strategies.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 22","pages":" 6483-6492"},"PeriodicalIF":6.1,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144016054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}