Analytical Chemistry最新文献_第2页

Tandem Bioluminescent Tests for Rapid Detection of Urinary Tract Infections and Antimicrobial Resistance Using a Portable Luminometer. 便携式发光计串联生物发光快速检测尿路感染和抗菌素耐药性

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-07-25 DOI: 10.1021/acs.analchem.5c03009

Sherwin N Reyes,Raymarcos Rodriguez,Amanda Murias,Emre Dikici,Sylvia Daunert,Sapna K Deo

{"title":"Tandem Bioluminescent Tests for Rapid Detection of Urinary Tract Infections and Antimicrobial Resistance Using a Portable Luminometer.","authors":"Sherwin N Reyes,Raymarcos Rodriguez,Amanda Murias,Emre Dikici,Sylvia Daunert,Sapna K Deo","doi":"10.1021/acs.analchem.5c03009","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c03009","url":null,"abstract":"Urinary tract infections (UTIs) are among the most common bacterial infections in the world. Additionally, increasing antimicrobial resistance (AMR) further complicates the task of effective therapy for UTIs and, thus, presents a serious public health hazard. The standard diagnosis for UTI and AMR requires access to laboratory infrastructures, well-trained personnel, and long periods of processing time, all of which are factors increasing healthcare costs and inappropriate antibiotic treatment. Here, we designed bioluminescence-based tests for UTI and AMR detection that can be performed in tandem using a portable luminometer in ∼30 min. To date, no methods have been reported that can simultaneously detect both UTI and AMR using the same detection principle and portable device as described here, offering a significant advantage in streamlining testing. Our UTI test is based on whole-cell bioluminescence assay, and AMR detection employs a modified ATP-based bioluminescence detection. Our test can detect UTI in 5 min at a bacterial level as low as 20,000 CFU/mL, which meets the clinically significant threshold of 100,000 CFU/mL. Antibiotic resistance was monitored using a modified ATP-based bioluminescence test within 30 min. The test showed no interference from potential urinary components, such as glucose, urea, commensal flora, or pH variations. A clinical sample (n = 8) analysis using these tests showed 100% accuracy in diagnosing UTI and AMR. These tandem tests using a portable bioluminescent reader could potentially minimize delays in diagnosis to allow immediate clinical decisions and optimization of treatment with antibiotics in clinical or community health settings.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"20 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144701264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Compound-Specific and Intramolecular δ¹⁵N Analysis of a Poly-Nitrogenous Amino Acid: Histidine. 多氮氨基酸组氨酸的化合物特异性和分子内δ15N分析。

IF 6.7 1区化学

Analytical Chemistry Pub Date : 2025-07-24 DOI: 10.1021/acs.analchem.5c01711

Charlotte Wing Man Lee, Mark A Altabet, Jesus Baca, Jason Barrera, Lin Zhang

{"title":"Compound-Specific and Intramolecular δ15N Analysis of a Poly-Nitrogenous Amino Acid: Histidine.","authors":"Charlotte Wing Man Lee, Mark A Altabet, Jesus Baca, Jason Barrera, Lin Zhang","doi":"10.1021/acs.analchem.5c01711","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01711","url":null,"abstract":"Histidine (HIS) is an essential amino acid (AA) with key physiological roles in metal chelation and proton buffering. Its three nitrogen (N) atoms─one α-amino and two in the imidazole side chain─are incorporated through distinct biosynthetic pathways and undergo different catabolic processes. Thus, its intramolecular δ15N values likely provide additional information on these pathways and associated N fluxes. Very few studies have reported molecular average δ15NHIS (δ15NHIS-Total) values, and there are no reported intramolecular δ15NHIS data for natural materials due to technical limitations of available methods. Here, we present a novel analytical approach for compound-specific and intramolecular δ15N values of poly-nitrogenous AAs using HIS as an example. This scheme can be adapted to obtain position-specific δ15N values of other poly-nitrogenous AAs such as glutamine. Underivatized HIS is separated by ion-exchange chromatography (IC) and divided into two aliquots. One fraction is fully oxidized to NO3- using UV-persulfate oxidation for δ15NHIS-Total measurement, while the other undergoes NaClO oxidation, selectively converting α-N and a minor fraction of side chain-N to NO2- at a known ratio. The δ15NHIS values of α-N (δ15NHIS-α) and side chain-N (δ15NHIS-s) are then calculated from these two results. Our findings reveal that α-N is consistently enriched in 15N relative to side chain-N in both commercial HIS powder (Δδ15Nα-s = ∼ +8‰) and biological samples (Δδ15Nα-s = ∼+3 to 25‰), likely due to preferential α-N catabolism via deamination. This finding supports the potential of studying diverse biosynthetic and catabolic processes of poly-nitrogenous AAs using intramolecular N isotope analysis.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":" ","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two-Dimensional Heterospectral Correlation Analysis Elucidates Photodegradation Pathways of Riverine Dissolved Organic Matter Using Excitation-Emission Matrix and Ultraviolet Spectroscopy. 二维异光谱相关分析利用激发-发射矩阵和紫外光谱阐明了河流溶解有机物的光降解途径。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-07-24 DOI: 10.1021/acs.analchem.5c01963

Tong Xiao,Junwen Hou,Shixiang Zhang,Dongping Liu,Hongjie Gao,Huibin Yu

{"title":"Two-Dimensional Heterospectral Correlation Analysis Elucidates Photodegradation Pathways of Riverine Dissolved Organic Matter Using Excitation-Emission Matrix and Ultraviolet Spectroscopy.","authors":"Tong Xiao,Junwen Hou,Shixiang Zhang,Dongping Liu,Hongjie Gao,Huibin Yu","doi":"10.1021/acs.analchem.5c01963","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01963","url":null,"abstract":"Photodegradation of dissolved organic matter (DOM) in aquatic environments can alter spectral fingerprints beyond microbial composition changes, which reduces traceback accuracy. Here, photodegradation effects on the fluorescence properties of DOM from typical sources were investigated using fluorescence excitation-emission matrix spectroscopy (EEMs), i.e., sheep excrement (EXC), urban sewage (URB), petrochemical wastewater (IND), and riparian topsoil (tDOM), over a 12-day photodegradation experiment. Six components (C1-C6) were identified: C1: lignin-derived intermediates, C2: tryptophan-like, C3: fulvic-like, C4/C6: tyrosine-like, and C5: photodegradation byproducts. Interestingly, C1, C4 and C6 dominated in EXC/IND-DOM, C5 in tDOM, and C2 in URB. Based on hetero-2D correlation spectroscopy (EEMs-UV) and moving window (MW) analysis, we found that protein-like in EXC-DOM and tDOM and amine-like in IND-DOM containing phenolic, aromatic, and alcoholic-hydroxyl are photosensitive and deeply decompose in 0-4 days during the photodegradation, while protein-like in URB-DOM is continuously decomposed. Moreover, lignin-derived was rapidly decomposed in 0-2 days during the EXC-DOM, URB-DOM, and tDOM degradation. According to the structural equation model, protein-like in EXC-DOM could be deeply degraded indirectly through C5, whereas that in IND, URB and tDOM might be directly degraded. Furthermore, lignin-derived might be directly decomposed in EXC, tDOM, and URB. These findings not only reveal the photodegradation mechanism of DOM from different sources but also are conducive to traceability in natural and engineered water bodies.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"44 12 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144693439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic Imaging of Solvent Exchange-Induced Interlayer Shifting in Single Two-Dimensional Covalent Organic Frameworks. 单二维共价有机骨架中溶剂交换引起的层间位移的动态成像。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-07-24 DOI: 10.1021/acs.analchem.5c02684

Xiaojuan Li,Qiongyao Ning,Jiaqi Yao,Sitong Long,Jichuan Huo,Yi He,Rui Liu

{"title":"Dynamic Imaging of Solvent Exchange-Induced Interlayer Shifting in Single Two-Dimensional Covalent Organic Frameworks.","authors":"Xiaojuan Li,Qiongyao Ning,Jiaqi Yao,Sitong Long,Jichuan Huo,Yi He,Rui Liu","doi":"10.1021/acs.analchem.5c02684","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c02684","url":null,"abstract":"The interlayer shifting of two-dimensional (2D) covalent organic frameworks (COFs) is a critical issue affecting their sorption and catalytic properties. However, current analytical tools often measure static and averaged stacking properties from bulk samples, restricting a comprehensive understanding of the interlayer shifting process in individual 2D COFs. Here, we report real-time dynamic imaging of the solvent exchange-induced interlayer shifting in single 2D COFs using in situ dark-field optical microscopy (DFM). The increase in pore size from quasi-AB stacking to AA stacking in triphenylbenzene (TPB)-terephthaldehyde-COF and TPB-dimethoxyterephthaldehyde-COF in organic solvent and water facilitates the accommodation of more guest molecules, leading to a higher scattering intensity. By using the grayscale intensity of DFM images as a quantitative indicator, we visually observe that the solvent exchange-triggered interlayer shifting dynamics not only exhibits a significant particle-to-particle heterogeneity but also is sensitive to the solvent viscosity and interlayer interaction strength of 2D COFs. Moreover, theoretical simulations elucidate that this interlayer shifting process is governed by crystal stacking energies and follows a thermodynamically preferred pathway during the water-to-organic exchange process. This imaging methodology provides a useful way for direct observation of solvent exchange-induced interlayer shifting in single 2D COFs and understanding its dynamics.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"17 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144693442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

β-Lactoglobulin Amyloid Fibrils-Poly(vinyl alcohol)-AgNPs Hydrogel as a Surface-Enhanced Raman Substrate for All-in-One Rapid Separation, Enrichment, and Detection of Small Molecules in Food Samples. β-乳球蛋白-淀粉样蛋白原纤维-聚乙烯醇-AgNPs水凝胶作为一种表面增强拉曼底物，用于食品样品中小分子的快速分离、富集和检测。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-07-24 DOI: 10.1021/acs.analchem.5c02529

Yaoyao Zhang,Xinxin Zhang,Chaotao Chen,Jingxiang Wang,Fucun Wei,Hanqi Liu,Fangkai Du,Lirong Jiang,Weiwei Zhu,Xuecai Tan,Rihui Su,Jun Yan

{"title":"β-Lactoglobulin Amyloid Fibrils-Poly(vinyl alcohol)-AgNPs Hydrogel as a Surface-Enhanced Raman Substrate for All-in-One Rapid Separation, Enrichment, and Detection of Small Molecules in Food Samples.","authors":"Yaoyao Zhang,Xinxin Zhang,Chaotao Chen,Jingxiang Wang,Fucun Wei,Hanqi Liu,Fangkai Du,Lirong Jiang,Weiwei Zhu,Xuecai Tan,Rihui Su,Jun Yan","doi":"10.1021/acs.analchem.5c02529","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c02529","url":null,"abstract":"A β-lactoglobulin amyloid fibrils-poly(vinyl alcohol)-Ag nanoparticles (β-LAFs-PVA-AgNPs) hydrogel was prepared as a surface-enhanced Raman scattering (SERS) substrate based on poly(vinyl alcohol) (PVA) with adsorbent β-lactoglobulin amyloid fibrils (β-LAFs) structured into the network structure of the hydrogel using the immersion reduction method. Due to the high swelling and contraction properties of the hydrogel in different states and the \"sieving\" nature of its network cavities, small molecular target analytes can more easily enter the space-confined hydrogel and rapidly bind with the amide bonds of the protein structure. During the contraction process of the hydrogel, the target analytes are enriched, and the Ag nanoparticles (AgNPs) come closer together to form Raman \"hot spots\", generating stronger SERS signals. The SERS performance of this hydrogel was evaluated using thiram and ibuprofen as target molecules. The results showed high detection sensitivity with limits of 37.1 μg/L for thiram and 85.6 μg/L for ibuprofen, respectively, and the entire sample pretreatment and detection process is completed within 11 min. The substrate also demonstrated excellent selectivity, uniformity, stability, and reproducibility. When applied to detect thiram in fruits and vegetables and ibuprofen in herbal tea, the recovery rates ranged from 90.1% to 114.7%, with the relative standard deviations (RSDs) of 0.61% to 5.8%. The SERS results matched those from HPLC analysis. This method offers simple and rapid sample pretreatment, high sensitivity, strong adsorption capacity for organic pollutants, and good selectivity, making it a promising candidate for on-site rapid detection applications.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"31 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144693441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cascade Catalytic Iron Phosphate Nanozyme-Driven Signal Amplification in S-Scheme AgBr/La-BiOBr-OV for Sensitive Dual-Channel Microfluidic PEC Detection of CA15-3 and CA125. 级联催化磷酸铁纳米酶驱动的S-Scheme AgBr/La-BiOBr-OV信号放大用于敏感的双通道微流体PEC检测CA15-3和CA125。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-07-24 DOI: 10.1021/acs.analchem.5c02951

Yu Du,Hao Geng,Jingui Chen,Xu Sun,Tingting Wu,Hongmin Ma,Zhongfeng Gao,Qin Wei

{"title":"Cascade Catalytic Iron Phosphate Nanozyme-Driven Signal Amplification in S-Scheme AgBr/La-BiOBr-OV for Sensitive Dual-Channel Microfluidic PEC Detection of CA15-3 and CA125.","authors":"Yu Du,Hao Geng,Jingui Chen,Xu Sun,Tingting Wu,Hongmin Ma,Zhongfeng Gao,Qin Wei","doi":"10.1021/acs.analchem.5c02951","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c02951","url":null,"abstract":"An innovative dual-channel microfluidic photoelectrochemical (PEC) immunosensor was constructed for simultaneous determination of carbohydrate antigen 15-3 (CA15-3) and cancer antigen 125 (CA125). Herein, AgBr-sensitized La-doped BiOBr with surface oxygen vacancies (AgBr/La-BiOBr-OV) was synthesized as a photoactive material to provide a stable photocurrent. Constructing an S-scheme heterojunction with AgBr and BiOBr facilitates the effective separation of photogenerated carriers. Meanwhile, the introduction of La and oxygen vacancies extends the range of visible-light absorption and improves the light utilization. To strengthen the PEC performance, a cascade catalytic amplification strategy was introduced using iron-phosphate-based (FePOs) nanozymes. These nanozymes exhibit dual enzyme-mimicking activities, including catalase (CAT)-like and superoxide dismutase (SOD)-like functions, enabling the sequential disproportionation of •O2- into H2O2 and O2, followed by the decomposition of H2O2 into O2 and H2O. The generated oxygen acts as an efficient electron acceptor, promoting the removal of photogenerated electrons and consequently boosting the PEC response. Under optimized conditions, the developed immunosensor enables highly sensitive quantification of CA15-3 (0.0005-200 U/mL) and CA125 (0.001-200 U/mL), with corresponding limits of detection (LOD) of 0.00016 and 0.0003 U/mL, respectively. This study provides a promising approach to constructing efficient S-scheme photoactive heterostructures and offers new insights into nanozyme-based cascade amplification for high-performance PEC biosensing.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"53 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144701262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ru(phen)32+-Sensitized GaN-Gated Organic Photoelectrochemical Transistor for Sensitive and Selective Detection of Human Papillomavirus DNA. Ru(phen)32+敏化gan门控有机光电电化学晶体管用于人乳头瘤病毒DNA的灵敏和选择性检测。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-07-24 DOI: 10.1021/acs.analchem.5c03789

Xuran Tian,Zhiqin Zhang,Yulan Zeng,Zhonghui Chen,Yue Lin,Fang Luo,Bin Qiu,Jian Wang,Zhenyu Lin

{"title":"Ru(phen)32+-Sensitized GaN-Gated Organic Photoelectrochemical Transistor for Sensitive and Selective Detection of Human Papillomavirus DNA.","authors":"Xuran Tian,Zhiqin Zhang,Yulan Zeng,Zhonghui Chen,Yue Lin,Fang Luo,Bin Qiu,Jian Wang,Zhenyu Lin","doi":"10.1021/acs.analchem.5c03789","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c03789","url":null,"abstract":"Benefiting from the well-matched energy levels between Ru(phen)32+ and GaN, and the ability of Ru(phen)32+ to intercalate into the grooves of double-stranded DNA (dsDNA), an innovative organic photoelectrochemical transistor (OPECT)-based biosensor has been developed for the detection of human papillomavirus 16 (HPV-16), which was chosen as a model target. Trigger DNA immobilized on the gold-coated GaN surface can initiate a hybridization chain reaction (HCR), generating long-stranded dsDNA, which can be used to load a large amount of Ru(phen)32+. The produced dsDNA can also serve as a π-π-stacked hole-transporting nanowire, enhancing the efficiency of the hole transport between GaN and Ru(phen)32+. Under light irradiation, efficient energy level matching between GaN and Ru(phen)32+ promotes the transport efficiency of photogenerated carriers, leading to a significant increase in the drain-source current (IDS). In the presence of HPV-16, the CRISPR/Cas12a system is activated and the immobilized trigger DNA is cleaved, thereby preventing the formation of dsDNA through HCR. As a result, a smaller amount of Ru(phen)32+ is immobilized on the electrode surface, leading to a reduction in the output signal. The signal change exhibits a linear relationship with the target concentration in the range of 10.00 fM to 10.00 nM, achieving a detection limit as low as 2.88 fM, which is much better than that of the corresponding photoelectrochemical (PEC) biosensor. The system demonstrates excellent sensitivity and specificity in clinical sample analysis.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"25 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144693440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supercharging Proteins to Near the Theoretical Limit via Reduced Pressure Ionization. 通过减压电离使蛋白质增压到接近理论极限。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-07-24 DOI: 10.1021/acs.analchem.5c02846

Chong Zhang,Jake P Violi,Christopher S Hansen,William A Donald

{"title":"Supercharging Proteins to Near the Theoretical Limit via Reduced Pressure Ionization.","authors":"Chong Zhang,Jake P Violi,Christopher S Hansen,William A Donald","doi":"10.1021/acs.analchem.5c02846","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c02846","url":null,"abstract":"Electrospray ionization (ESI) is well-known for generating multiply charged protein ions. Higher charge states enhance tandem mass spectrometry (MS/MS) by improving fragmentation efficiency and increasing sequence coverage. However, the extent of protein charging can be limited by many factors including proton transfer reactions with ambient gases. Here, we demonstrate that droplet desolvation under reduced pressure significantly increases protein charge states, especially when combined with chemical supercharging additives such as 1,2-butylene carbonate. Using reduced pressure nanoelectrospray ionization (250 Torr), the maximum observed charge states of cytochrome c (12.6 kDa) and myoglobin (16.7 kDa) increased from 24+ and 32+ (under ambient conditions) to 30+ and 40+, respectively, within 15% of their predicted in vacuo charge state limits. Enhanced charging was also achieved for larger proteins. For example, carbonic anhydrase (29 kDa) and bovine serum albumin (BSA, 66.5 kDa) reached charge states of 62+ and 112+ under reduced pressure, compared to 46+ and 92+ at ambient pressure. These results demonstrate that combining reduced pressure desolvation with chemical supercharging is an effective approach for increasing protein charge states in ESI to near the theoretical limit, and should offer advantages for the top-down analysis of large biomolecules.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"767 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144693443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of a Scanning Ion-Conductance Microscope for Tip-Enhanced Raman Spectroscopy. 尖端增强拉曼光谱扫描离子电导显微镜的构建。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-07-24 DOI: 10.1021/acs.analchem.5c02986

Xing He,Leonardo Scarabelli,Naihao Chiang

{"title":"Construction of a Scanning Ion-Conductance Microscope for Tip-Enhanced Raman Spectroscopy.","authors":"Xing He,Leonardo Scarabelli,Naihao Chiang","doi":"10.1021/acs.analchem.5c02986","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c02986","url":null,"abstract":"Tip-enhanced Raman spectroscopy (TERS) has been developed by using atomic force microscopy and scanning tunneling microscopy since its birth in the early 2000s. Because, in principle, any scanning probe microscopy (SPM) can be combined with TERS, there is an increasing interest in extending TERS to other SPMs, especially the liquid-phase ones, targeting applications in biological or catalytic systems. We present the design, construction, and characterization of a laser-coupled scanning ion-conductance microscope (SICM) integrated with TERS for near-field spectroscopy under liquid environments. Key design elements include a full sample-scanning SICM, optics for TERS, and noise-isolating methods. The SICM-TERS system demonstrates direct-current and alternating-current SICM imaging modes on polydimethylsiloxane (PDMS) structures. Furthermore, the TERS capability was tested using silica-nanosphere-grafted PDMS. This work illustrates the feasibility and performance of SICM-TERS as a versatile tool for label-free nanoscale spectroscopy, opening new possibilities for real-time, in situ analysis of soft matter, electrochemical, and biological systems.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"12 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144701151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasensitive Quantum Weak Measurement Biochemical Sensor via a Porous Anodic Alumina Nanostructure. 基于多孔阳极氧化铝纳米结构的超灵敏量子弱测量生化传感器。

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-07-23 DOI: 10.1021/acs.analchem.5c01860

Jiao Ren,Mengyao Sun,Qin Qin,Changwei Ma,Pengpeng Xie,Xiaoxue Xu,Guiqiang Wang

{"title":"Ultrasensitive Quantum Weak Measurement Biochemical Sensor via a Porous Anodic Alumina Nanostructure.","authors":"Jiao Ren,Mengyao Sun,Qin Qin,Changwei Ma,Pengpeng Xie,Xiaoxue Xu,Guiqiang Wang","doi":"10.1021/acs.analchem.5c01860","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01860","url":null,"abstract":"In this paper, an ultrasensitive quantum weak measurement biochemical sensor based on porous anodic alumina (PAA) was first proposed and realized. The PAA film has the advantages of a regular hexagonal arrangement of pores, a high specific surface area, and low cost, which is a significant biosensing platform. By optimizing the structure parameters such as pore size and thickness of the PAA film, the biochemical sensor achieves a refractive index sensitivity of as high as 25 326 nm/RIU with a corresponding resolution of as low as 1.4 × 10-7 RIU. The combination of the PAA film and weak measurement technology amplifies the sensing changes of small phase differences, thus realizing a highly sensitive response to the changes of the sensing medium. The biochemical sensor is capable of detecting biomolecules, such as glucose and proteins, with low detection limits and high specificity. Molecular testing validates that the sensor has a limit of detection as low as 3.9 mg/L for glucose small molecules and 112 pM for bovine serum albumin (BSA) biomacromolecules. The specificity testing validates that the biosensor is capable of specifically recognizing IgG proteins with high sensitivity, and a limit of detection as low as 8.4 ng/mL was achieved. As a result, the proposed PAA-based reflection-type quantum weak measurement biochemical sensor has the advantages of robustness and simplicity of structure, providing a versatile platform for the next generation of high-sensitivity biochemical sensing technology.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"27 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144693444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0