Analytical Chemistry最新文献_第2页

Full Spectral Overlap to Enhanced Fluorescence Quenching Ability by Using Covalent Organic Frameworks as a Springboard of Quencher for the Turn-on Fluorescence Immunoassay

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2024-12-22 DOI: 10.1021/acs.analchem.4c03915

Yuanyuan Cheng, Xiaomin Li, Shouyu Xue, Xuechi Yin, Yuechun Li, Jianlong Wang, Daohong Zhang

{"title":"Full Spectral Overlap to Enhanced Fluorescence Quenching Ability by Using Covalent Organic Frameworks as a Springboard of Quencher for the Turn-on Fluorescence Immunoassay","authors":"Yuanyuan Cheng, Xiaomin Li, Shouyu Xue, Xuechi Yin, Yuechun Li, Jianlong Wang, Daohong Zhang","doi":"10.1021/acs.analchem.4c03915","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c03915","url":null,"abstract":"According to the fluorescence internal filtering effect (IFE), the more the absorption spectrum of the quencher overlaps with the excitation and emission spectra of the fluorescent substance, the better the quenching effect and, correspondingly, the more significant and sensitive the contrast becomes when the fluorescence is turned on. Thus, in the competitive fluorescence-quenching lateral flow immunoassays (FQ-LFIAs), the fluorescence quencher with an outstanding optical property is of great importance. Herein, gold nanoparticles (AuNPs) and polydopamine (PDA) coengineered covalent organic frameworks (COF/Au@PDA) were synthesized as a fluorescence quencher to increase spectral overlap. Thanks to the excellent visible light absorption of COF with donor–acceptor (D-A) structure, the localized surface plasmon resonance (LSPR) capability of AuNPs, and the broad light absorption of the PDA layer, the COF/Au@PDA exhibits intense absorption and a full spectral overlap toward aggregation-induced emission luminous (AIE) dots. Thereafter, COF/Au@PDA, with its immense potential to completely quench the fluorescence of AIE dots through primary IFE and secondary IFE, was applied to a bimodal LFIA platform for verification with a nitrofurazone metabolite as a model analyte. As expected, the detection sensitivity of the COF/Au@PDA-based FQ-LFIA (turn-on) is improved by 6-fold compared with that of the colorimetric (CM)-LFIA (turn-off). Further, ChatGpt was used to improve the assay accuracy and sensitivity, utilizing its high sensitivity to subtle changes in LFIA signals, especially for weak signals that are indeterminate with the naked eye. This work offers a potential approach for building a high-performance fluorescence quencher in the FQ-LFIA and indicates the potential for the application of artificial intelligence in highly sensitive LFIAs.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"24 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LRET-Based Simultaneous Detection of Dual miRNAs via Multitrap Optical Tweezers Assisted Suspension Array Tagged by Two Different Luminescent Quenchable UCNPs Combining CRISPR/Cas12a Amplification

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2024-12-22 DOI: 10.1021/acs.analchem.4c04895

He Yu, Peng-Fei Xu, Yang Liu, Zeng-Shuai Jia, Yu-Yao Li, Hong-Wu Tang

{"title":"LRET-Based Simultaneous Detection of Dual miRNAs via Multitrap Optical Tweezers Assisted Suspension Array Tagged by Two Different Luminescent Quenchable UCNPs Combining CRISPR/Cas12a Amplification","authors":"He Yu, Peng-Fei Xu, Yang Liu, Zeng-Shuai Jia, Yu-Yao Li, Hong-Wu Tang","doi":"10.1021/acs.analchem.4c04895","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c04895","url":null,"abstract":"Nowadays, optical tweezers play a vital role not only in optical manipulation but also in bioassay. As principal optical trapping objects, microbeads can combine optical tweezers with suspension array technology, with amply focused laser beams and adequately concentrated tags contributing to highly sensitive detection. In view of the inefficiency of conventional single-trap optical tweezers, multitrap systems are developed. Here, green- and blue-emitting core–shell–shell upconversion nanoparticles (UCNPs) are adopted to encode microbeads and determine dual miRNAs, with the internal shells leading the luminescence process to facilitate quenching through luminescence resonance energy transfer (LRET). Utilizing the trans cleavage of CRISPR/Cas12a, quenched luminescence signals are recovered and amplified, causing further enhanced detection sensitivity. Ultimately, limits of detection (LOD) of 17 and 22 aM are obtained with excellent specificities verified. Furthermore, dual miRNAs from MCF-7, A549, and MCF-10A cells are extracted and detected, with results consistent with those obtained by PCR. Notably, miR-155 in MCF-7 and A549 cells is detectable at the single-cell level. Thus, the differences in the measured miRNA levels between MCF-7 and MCF-10A cells imply the potential of this method to discriminate breast cancer cells from epithelial cells despite the difficulty in distinguishing different cancer cells due to similar miRNA levels.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"14 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LipoCLEAN: A Machine Learning Filter to Improve Untargeted Lipid Identification Confidence

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2024-12-22 DOI: 10.1021/acs.analchem.4c04040

Steven L. Tavis, Matthew J. Keller, Andrew J. Stai, Tomás A. Rush, Robert L. Hettich

引用次数: 0

Active-Targeted ICG for Surgical Navigation and Fluorescence-Guided Laparoscopic Photothermal Ablation in Pancreatic Ductal Adenocarcinoma

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2024-12-22 DOI: 10.1021/acs.analchem.4c04575

Lei Zhou, Manxiong Dai, Jiahao Zhou, Xingyang Zhao, Zixiong Liu, Hao Bu, Yang Zhou, Yan Liao, Hongwen Liu, Wei Cheng, Kang Chen

{"title":"Active-Targeted ICG for Surgical Navigation and Fluorescence-Guided Laparoscopic Photothermal Ablation in Pancreatic Ductal Adenocarcinoma","authors":"Lei Zhou, Manxiong Dai, Jiahao Zhou, Xingyang Zhao, Zixiong Liu, Hao Bu, Yang Zhou, Yan Liao, Hongwen Liu, Wei Cheng, Kang Chen","doi":"10.1021/acs.analchem.4c04575","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c04575","url":null,"abstract":"Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy, but there is limited improvement in its treatment. Near-infrared fluorescence (NIRF) imaging could potentially address the clinical challenges of PDAC. Indocyanine green (ICG) has been widely used in clinical practice; however, its short half-life and lack of active targeting greatly limit its application in pancreatic surgery. In this study, the active targeting peptide KTLLPTP (which actively recognizes PDAC cell surface overexpression Plectin-1) was modified to the ICG to create the novel contrast agent ICG-PTP, which actively targets PDAC cells. It was successfully applied to the NIRF imaging of the PDAC orthotopic mice model, achieving an improved tumor signal background ratio (T/N ratio) of 4.28, compared to 2.34 in the free ICG group. Next, Fluorescence-guided excision of subcutaneous/orthotopic PDAC using ICG-PTP was performed, accurately identifying the tumor margin and significantly facilitating resection efficiency. Finally, PDAC metastases were identified, and interventional photothermal ablation (iPTA) was performed under fluorescence laparoscope guidance. ICG-PTP exhibits good biosafety and clinical transitional potential. Thus, they can provide surgeons with efficient real-time tumor information and offer new treatment strategies for metastases. Accordingly, modification of probes for clinical use and adaptation studies of current equipment are the current focus.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"36 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunoaffinity Intact Top-Down Mass Spectrometry for Quantification of Neuron-Specific Enolase Gamma, a Low-Abundance Protein Biomarker

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2024-12-22 DOI: 10.1021/acs.analchem.4c04677

Sebastian A. H. van den Wildenberg, Sylvia A. A. M. Genet, Maarten A. C. Broeren, Joost L. J. van Dongen, Maxime C.M. van den Oetelaar, Luc Brunsveld, Volkher Scharnhorst, Daan van de Kerkhof

{"title":"Immunoaffinity Intact Top-Down Mass Spectrometry for Quantification of Neuron-Specific Enolase Gamma, a Low-Abundance Protein Biomarker","authors":"Sebastian A. H. van den Wildenberg, Sylvia A. A. M. Genet, Maarten A. C. Broeren, Joost L. J. van Dongen, Maxime C.M. van den Oetelaar, Luc Brunsveld, Volkher Scharnhorst, Daan van de Kerkhof","doi":"10.1021/acs.analchem.4c04677","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c04677","url":null,"abstract":"Quantification of intact proteins in serum by liquid chromatography high-resolution mass spectrometry (HRMS) may be a useful alternative to bottom-up LC-MS or conventional ligand binding assays, due to reduced assay complexity and by providing additional information, such as isoform differentiation or detection of post-translational modifications. The 47.2 kDa lung cancer tumor marker neuron-specific enolase γ (NSEγ) was quantified in a clinically relevant concentration range of 6.25 to 100 ng/mL in NSE-depleted human serum using magnetic bead immunoprecipitation coupled to LC-high-resolution quadrupole-time-of-flight MS. The novelty of the described approach is in the combined setup of immunoaffinity extraction and the use of a full-length NSEγ calibrator and labeled NSEγ internal standard (IS) to reliably quantify the post-translationally acetylated form of this protein tumor marker in a top-down proteomics workflow. Isolation parameters and quantification using deconvolution and reconstructed extracted ion chromatograms were evaluated, and the development of a suitable liquid chromatography method was demonstrated. Various validation parameters were determined using both quantification methods, both showing acceptable performance. Additionally, deconvolution-based quantification enabled an accurate mass determination. The developed method was compared to a commercially available ECLIA and showed good correlation in sera of patients suspected of lung cancer. This assay may form the starting point for the development of a reference method for the standardization of immunoassays.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"38 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycosylation-Targeting Aptamer for the Feasible Construction of a Dual Aptamer-Based Plasmonic Immunosandwich Assay in Cancer Diagnostics

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2024-12-22 DOI: 10.1021/acs.analchem.4c03770

Junyang Chen, Pengfei Ma, Jiayu Xu, Mingxi Zang, Wei Li

{"title":"Glycosylation-Targeting Aptamer for the Feasible Construction of a Dual Aptamer-Based Plasmonic Immunosandwich Assay in Cancer Diagnostics","authors":"Junyang Chen, Pengfei Ma, Jiayu Xu, Mingxi Zang, Wei Li","doi":"10.1021/acs.analchem.4c03770","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c03770","url":null,"abstract":"Fibroblast activation protein (FAP) is an important antigen in the tumor microenvironment, which plays a crucial role in promoting extracellular matrix remodeling and tumor cell metastasis. A circulating form of soluble FAP has also been identified in the serum, becoming a biomarker for pan-cancer diagnosis and prognosis. However, the current peptide substrate-based enzymatic activity detection or antibody-dependent detection methods have been hindered by insufficient selectivity and complex operations, so it is valuable to develop effective nucleic acid aptamers as FAP affinity ligands. In order to deeply explore the biomimetic recognition technology, this study proposed an elaborate aptamer screening strategy for targeting the protein characteristic structure. Taking the glycosylation of the FAP protein as a target, four FAP-specific aptamers with high performance were successfully generated. Further, using the champion aptamer as a recognition tool and combining it with ultrasensitive detection technology-surface enhanced Raman scattering (SERS), a novel dual aptamer-based sandwich sensor was constructed for the rapid determination of FAP. Due to the dual-specific recognition of the orthogonal aptamer pair, the sandwich method obviously improved the selectivity to FAP protein, with a maximum cross-reactivity of less than 8% and a quantitation limit of 100 pg/mL. It was conveniently applied in high-sensitive and high-selective detection of serum FAP in cancer patient samples. Therefore, the research of this study not only opens new access for the selection of antiglycan aptamers but also boosts the application of the FAP aptamer as a recognition tool in cancer diagnostics.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"107 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trifluoroacetic Acid as a Molecular Probe for the Dense Phase in Liquid–Liquid Phase-Separating Peptide Systems

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2024-12-22 DOI: 10.1021/acs.analchem.4c03444

Jessica Lim, SzeYuet Chin, Ali Miserez, Kai Xue, Konstantin Pervushin

引用次数: 0

Untargeted Spatial Metabolomics and Spatial Proteomics on the Same Tissue Section

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2024-12-21 DOI: 10.1021/acs.analchem.4c04462

Gregory W. Vandergrift, Marija Veličković, Le Z. Day, Brittney L. Gorman, Sarah M. Williams, Bindesh Shrestha, Christopher R. Anderton

{"title":"Untargeted Spatial Metabolomics and Spatial Proteomics on the Same Tissue Section","authors":"Gregory W. Vandergrift, Marija Veličković, Le Z. Day, Brittney L. Gorman, Sarah M. Williams, Bindesh Shrestha, Christopher R. Anderton","doi":"10.1021/acs.analchem.4c04462","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c04462","url":null,"abstract":"An increasing number of spatial multiomic workflows have recently been developed. Some of these approaches have leveraged initial mass spectrometry imaging (MSI)-based spatial metabolomics to inform the region of interest (ROI) selection for downstream spatial proteomics. However, these workflows have been limited by varied substrate requirements between modalities or have required analyzing serial sections (i.e., one section per modality). To mitigate these issues, we present a new multiomic workflow that uses desorption electrospray ionization (DESI)-MSI to identify representative spatial metabolite patterns on-tissue prior to spatial proteomic analyses on the same tissue section. This workflow is demonstrated here with a model mammalian tissue (coronal rat brain section) mounted on a poly(ethylene naphthalate)-membrane slide. Initial DESI-MSI resulted in 160 annotations (SwissLipids) within the METASPACE platform (≤20% false discovery rate). A segmentation map from the annotated ion images informed the downstream ROI selection for spatial proteomics characterization from the same sample. The unspecific substrate requirements and minimal sample disruption inherent to DESI-MSI allowed for an optimized, downstream spatial proteomics assay, resulting in 3888 ± 240 to 4717 ± 48 proteins being confidently directed per ROI (200 μm × 200 μm). Finally, we demonstrate the integration of multiomic information, where we found ceramide localization to be correlated with SMPD3 abundance (ceramide synthesis protein), and we also utilized protein abundance to resolve metabolite isomeric ambiguity. Overall, the integration of DESI-MSI into the multiomic workflow allows for complementary spatial- and molecular-level information to be achieved from optimized implementations of each MS assay inherent to the workflow itself.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"52 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intrinsic Chirality Modulation and Biosensing Application of Helical Gold Nanorods by Anisotropic Etching

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2024-12-21 DOI: 10.1021/acs.analchem.4c04208

Liang Tong Li, Shuai Wen, Qiu Yue Liu, He Feng Shi, Min Huang, Chen Liu, Lei Zhan, Xiao Hui Zhao, Hong Yan Zou, Cheng Zhi Huang, Jian Wang

{"title":"Intrinsic Chirality Modulation and Biosensing Application of Helical Gold Nanorods by Anisotropic Etching","authors":"Liang Tong Li, Shuai Wen, Qiu Yue Liu, He Feng Shi, Min Huang, Chen Liu, Lei Zhan, Xiao Hui Zhao, Hong Yan Zou, Cheng Zhi Huang, Jian Wang","doi":"10.1021/acs.analchem.4c04208","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c04208","url":null,"abstract":"The investigation of plasmonic chirality is a profound and intriguing topic, and the distinctive morphology of intrinsically chiral nanoparticles has prompted significant interest in the structure–activity relationship between particle morphology and chirality. In this work, the anisotropic etching of chiral helical gold nanorods (HGNRs) by a cetyltrimethylammonium bromide (CTAB)–HAuCl4 complex was observed with an interesting bidirectional variation of intrinsic chirality that initially enhanced and subsequently weakened, which was related with the diversity in CTAB distribution. In addition, an ultrasensitive and convenient sensing platform for acetylcholinesterase was developed based on the circular dichroism signal recovery of HGNRs caused by the dual inhibition of HGNR etching. The distinctive etching process and mechanism of chiral nanoparticles offer new insights into understanding the structural features and biochemical applications of the plasmonic intrinsic chirality, which could be applied to the acquisition of chiral nanoparticles and sensitive detection platform based on chiral signal changes.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"88 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Comprehensive Evaluation of Culture-Independent, Long Amplicon-Based Targeted Next-Generation Sequencing Methods for Predicting Antimicrobial Resistance in Tuberculosis 开发和全面评估基于长扩增片段的培养依赖性靶向新一代测序方法，用于预测结核病的抗菌药耐药性

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2024-12-21 DOI: 10.1021/acs.analchem.4c04166

Lulu Zhang, Xia Yu, Chi Zhang, Xin Zhang, Hairong Huang, Junping Peng

{"title":"Development and Comprehensive Evaluation of Culture-Independent, Long Amplicon-Based Targeted Next-Generation Sequencing Methods for Predicting Antimicrobial Resistance in Tuberculosis","authors":"Lulu Zhang, Xia Yu, Chi Zhang, Xin Zhang, Hairong Huang, Junping Peng","doi":"10.1021/acs.analchem.4c04166","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c04166","url":null,"abstract":"The great variety of antimicrobial resistance (AMR) profiles among tuberculosis (TB) patients necessitates a comprehensive detection method. This study developed culture-independent, long amplicon-based targeted next-generation sequencing (tNGS) methods for predicting AMR across 16 drugs within the Mycobacterium tuberculosis complex (MTBC). Multiplex PCR amplification was employed to enrich 20 gene regions, with sequencing performed on either the Oxford Nanopore Technologies (ONT) or Illumina platforms. Customized bioinformatics pipelines provide a streamlined process from raw data to clinician-friendly reports. The ONT tNGS method has been optimized, and its performance has been thoroughly evaluated, utilizing Q20+ chemistry in combination with the R10.4.1 flow cell. It requires only 15 high-quality reads per target gene to accurately identify all variants, with the turnaround time taking 4 h and 50 min. Studies confirmed that this method effectively identifies Mycobacterium species and was highly resistant to interference from other clinical pathogens. To ensure optimal coverage, it is recommended to input at least 500 copies of the genome and sequence 500MB of high-quality FASTQ data. Diagnostic performance evaluations demonstrate that this method achieves 98.35% concordance with phenotypic drug susceptibility testing (pDST) and is consistent with the results obtained from Xpert MTB/RIF assays. The design of long amplicons not only ensures comprehensive coverage of target regions but also simplifies primer design, facilitating compatibility with various sequencing platforms. Compared with previous studies, the optimized ONT tNGS method in this study significantly improves turnaround time, detection accuracy, and the comprehensive coverage of mutations associated with AMR.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"13 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0