Analytical Chemistry最新文献

{"title":"Elimination of Carryover Contamination during Nucleic Acid Amplification Based on Damaged Base Excision by DNA Repair Enzymes","authors":"Xiaonan Liu, Wenjing Hu, Huyun Zhou, Jiaxing Zhang, Mengyang Zhao, Yali Cui, Jiangwei Yan","doi":"10.1021/acs.analchem.5c01256","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01256","url":null,"abstract":"Nucleic acid amplification is a pivotal technology for gene investigation in molecular biology as well as an indispensable tool for nucleic acid-based analyses across various disciplines. However, the aerosolized amplicons render the subsequent reaction susceptible to carryover contamination, leading to serious false positive results, particularly in scenarios involving repeated amplification of identical sequences. Although the uracil-DNA glycosylase-based strategy can address this issue for natural DNA amplification, the uracil-containing DNA required for bisulfite conversion-based DNA methylation analysis fails to be analyzed because both aerosol and the intended template can be hydrolyzed. In this study, an innovative strategy for one-pot elimination of carryover contamination is established, applicable to both natural and uracil-containing DNA analyses using either thermal cycle-based or isotherm-based nucleic acid amplification. By incorporation of deoxyinosine triphosphate, hypoxanthine is inserted into amplicons, resulting in a different base composition from the original template. Hypoxanthine, a damaged base as the recognition site of endonuclease V, acts as a label for aerosol cleavage, while the intended template lacking hypoxanthine is unaffected. By systematic optimization, abundant aerosols are thoroughly hydrolyzed within a brief time. Despite the use of deoxyinosine triphosphate as an aberrant deoxyribonucleoside triphosphate, the amplification efficiency, sensitivity, and specificity are insusceptible. In addition, the hypoxanthine-containing amplicons can be analyzed not only for length identification by gel and capillary electrophoresis but also for sequence identification by sanger sequencing, pyrosequencing, and massively parallel sequencing. Moreover, the hypoxanthine-containing amplicons can be further used for cloning and restriction enzyme digestion, indicating the significant potential of this anti-aerosol strategy.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"1 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144211535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3-Hydroxybenzeneboronic Acid Enables Stable and Sensitive Dopamine Detection by Inhibiting Target Self-Polymerization","authors":"Zhiming Yang, Junqi Cheng, Fengniu Lu, Jingxin Yu, Yi Du, Zhiqin Yuan, Chao Lu","doi":"10.1021/acs.analchem.4c06246","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c06246","url":null,"abstract":"Resorcinol (Res) and Res-motif-containing molecules have been explored for the design and construction of fluorometric probes toward dopamine (DA) detection. However, the self-polymerization of DA under alkaline conditions competes with the probe–DA reaction, reducing the stability and sensitivity of detection. In this study, a target-triggered cascade reaction strategy has been explored for the development of DA detection probe based on the formation of borate ester intermediates. As a case study, 3-hydroxybenzeneboronic acid (HBBA) was exploited for stable and sensitive DA detection by inhibiting DA self-polymerization. The mechanism study disclosed that DA first reacts with HBBA and forms borate ester, which is then oxidized and hydroxylated to produce Res and DA, further forming fluorescent azamonardine compounds. Using HBBA as the probe, DA detection was achieved with a detection limit of 0.4 nM. In addition, the practical application of the HBBA probe was verified by accurate DA analysis in urine and cerebrospinal fluid samples.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"54 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144202377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Visual Perspective for Tracking of Larval Development, Aging and Antiaging Drugs Screening by Fluorescent Sensing MsrA","authors":"Jiayu Zeng, Ting Yang, Yuan Gao, Yunjia Shen, Songjiao Li, Hongshuai Zhang, Jia Zhou, Dan Cheng, Longwei He","doi":"10.1021/acs.analchem.4c07050","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c07050","url":null,"abstract":"Senescence is observed in various tissues during different physiological and pathological events such as tissue remodeling and disorders related aging. Senescent cells suffer from a decline in methionine sulfoxide reductase (MsrA), which is intimately linked to cell proliferation and longevity. Monitoring MsrA may contribute to senescence research in larval development and age-related disorders. However, only a limited number of MsrA fluorescent probes have been reported, and none of them have been applied to respond to MsrA specifically and recognize senescent cells. This project developed a novel fluorescent probe <b>SOMP</b> with high sensitivity (low detection limit, 8.9 ng/mL) and specificity (less interference of MsrB2 and MsrB3) to recognize MsrA. Cell proliferation and larval development of zebrafish can be evaluated with <b>SOMP</b> by monitoring MsrA. Moreover, the robust probe was successfully shown to monitor the process of aging by multiple aging models, which is also verified by immunostaining and flow cytometry. In addition, <b>SOMP</b> can be successfully used for high-throughput screening of MsrA-related antiaging native drugs at the cellular level. Curcumin’s antiaging effects were found to be linked to MsrA-mediated redox pathways for the first time. We believe that the probe <b>SOMP</b> and its interesting findings could offer significant insights for future research on research on cell proliferation, larval development, aging, and evaluating antiaging drugs.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"139 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144202379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Spectral Barcoding and Classification Approach for Complex Biological Samples Using Multiexcitation Raman Spectroscopy (MX-Raman)","authors":"George Devitt, Niall Hanrahan, Miguel Ramírez Moreno, Amrit Mudher, Sumeet Mahajan","doi":"10.1021/acs.analchem.5c00776","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00776","url":null,"abstract":"We report the development and application of a novel spectral barcoding approach that exploits our multiexcitation (MX) Raman spectroscopy-based methodology for improved label-free detection and classification of complex biological samples. To develop our improved MX-Raman methodology, we utilized post-mortem brain tissue from several neurodegenerative diseases (NDDs) that have considerable clinical overlap. For improving our methodology we used three sources of spectral information arising from distinct physical phenomena to assess which was most important for NDD classification. Spectral measurements utilized combinations of data from multiple, distinct excitation laser wavelengths and polarization states to differentially probe molecular vibrations and autofluorescence signals. We demonstrate that the more informative MX-Raman (532 nm–785 nm) spectra are classified with 96.7% accuracy on average, compared to conventional single-excitation Raman spectroscopy that resulted in 78.5% accuracy (532 nm) or 85.6% accuracy (785 nm) using linear discriminant analysis (LDA) on 5 NDD classes. By combining information from distinct laser polarizations we observed a nonsignificant increase in classification accuracy without the need of a second laser (785 nm–785 nm polarized), whereas combining Raman spectra with autofluorescence signals did not increase classification accuracy. Finally, by filtering out spectral features that were redundant for classification or not descriptive of disease class, we engineered spectral barcodes consisting of a minimal subset of highly disease-specific MX-Raman features that improved the unsupervised and cross-validated clustering of MX-Raman spectra. The results demonstrate that increasing spectral information content using our optical MX-Raman methodology enables enhanced identification and distinction of complex biological samples but only when that information is independent and descriptive of class. The future translation of such technology to biofluids could support diagnosis and stratification of patients living with dementia and potentially other clinical conditions such as cancer and infectious disease.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"7 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144202382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection and Analysis of Reactive Oxygen Species (ROS): Buffer Components Are Not Bystanders","authors":"Shubham Bansal, Muskan Gori, Joanna Afokai Quaye, Giovanni Gadda, Binghe Wang","doi":"10.1021/acs.analchem.4c07070","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c07070","url":null,"abstract":"Reactive oxygen species (ROS) play critical roles in pathophysiological processes. Therefore, there is widespread interest in learning ROS concentrations under various conditions. However, literature numbers in ROS concentration vary significantly, and most cannot be readily compared against each other, largely because of the lack of understanding of the effects of various factors that significantly impact the experimental outcome. In this study, we examine an overlooked factor: the chemical reactivity of commonly used organic buffer molecules toward ROS and how such reactivity affects the results interpretation. Specifically, we examined HEPES, Tris, MES, citrate, ammonium acetate, and phosphate-buffered saline (PBS) and found that most organic buffer components can rapidly consume NaOCl (the second most abundant ROS) and/or directly interact with certain ROS probes such as a boronate for H₂O₂ determination, leading to significant errors in experimental findings and interpretations of results. For example, 20 mM HEPES, MES, ammonium acetate, and Tris are found to consume 1 mM hypochlorite within 1 s, leading to false negative results. Additionally, these organic buffer components have been found to cause false negative results in the detection of ONOO^– when using a boronate-based probe. As such, these organic buffers should be avoided in the determination of ROS concentrations. We use these examples to draw attention to the profound effects of buffer components on ROS detection and examine chemistry issues in detail. We hope the findings described will lead to improved rigor in designing ROS experiments by considering factors that were previously considered as nothing but bystanders or benign.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"13 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144202380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Digital Microfluidic Platform for the Microscale Production of Functional Immune Cell Therapies.","authors":"Samuel R Little, Niloufar Rahbari, Mehri Hajiaghayi, Fatemeh Gholizadeh, Fanny-Mei Cloarec-Ung, Joel Phillips, Hugo Sinha, Alison Hirukawa, David J H F Knapp, Peter J Darlington, Steve C C Shih","doi":"10.1021/acs.analchem.4c06911","DOIUrl":"10.1021/acs.analchem.4c06911","url":null,"abstract":"<p><p>Genetically engineering human immune cells has been shown to be an effective approach for developing novel cellular therapies to treat a wide range of diseases. To expand the scope of these cellular therapies while solving persistent challenges, extensive research and development is still required. Here we use a digital microfluidic enabled electroporation system (referred to as triDrop) specifically designed to mitigate harm during electroporation procedures and compare against two state-of-the-art commercially available systems for the engineering of primary human T cells. We describe the ability to use triDrop for highly efficient transfection with minimal reagent consumption while preserving a healthy transcriptomic profile. Finally, we show for the first time the ability to use a digital microfluidic platform for the miniaturized production of Chimeric Antigen Receptor (CAR) T cell therapies demonstrating how this novel system can lead to a 2-fold improvement in immunotherapeutic functionality compared to gold standard methods while providing up to a 20-fold reduction in cost. These results highlight the potential power of this system for automated, rapid, and affordable next-generation cell therapy R&D.</p>","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":" ","pages":"11026-11034"},"PeriodicalIF":6.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144100966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrodynamic Chromatography with Deterministic Lateral Displacement Effect","authors":"Valentina Biagioni","doi":"10.1021/acs.analchem.5c00947","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00947","url":null,"abstract":"Hydrodynamic chromatography (HDC) is a flow-driven passive method for separating micrometric/nanometric particles based on the interaction between a nonuniform velocity profile and Brownian diffusion, which causes particles of different size to migrate at different average velocity throughout the separation column. Despite its conceptual simplicity and relative ease of implementation, HDC remains to date an underutilized technique in view of the lengthy channels and large operational times required. In the search for optimal geometries enhancing separation efficiency, micro-Pillar Array Columns (μPACs), constituted by a doubly periodic obstacle lattice aligned with the direction of the flow, have been successfully proposed and tested. The aim of this article is to show that a further improvement of HDC efficiency in μPACs is possible by enforcing a symmetry breakup, where the lattice is misaligned by an angle θ_l with respect to the flow direction. The mismatch between the flow direction and the lattice axes triggers a new separation mechanism, referred to as Deterministic Lateral Displacement (DLD), which causes particles of different size to migrate along different directions through the lattice. So far, DLD has been enforced exclusively in continuous separations run under steady-state conditions.. If an unsteady (chromatographic) operating mode in a slanted μPACs is enforced, differences in migration velocities and migration angles act simultaneously as two independent mechanisms. Theoretical/numerical evidence is provided, showing that the synergy between the two separation drives can shorten device lengths and analysis times by a factor of 10 or even higher (depending on the analytical target) when compared to plain-HDC. The results presented are based on an advection-diffusion template enforcing the classical excluded-volume model to account for particle–wall interactions, an approach previously validated against experimental data by different research groups, both in standard μPACs-HDC and in continuous DLD devices. Numerical results of the average particle migration angle and velocity magnitude are obtained by two independent (Eulerian and Lagrangian) computational approaches. A case study of geometry is used throughout to illustrate the concrete implementation of the method for a multidispersed mixture of particles of five nominal diameters ranging from 1 to 1.6 μm.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"31 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144202383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shining Light on DNA Mutations through Machine Learning-Augmented Vibrational Spectroscopy","authors":"Vikas Yadav, Tripti Ahuja, Himanshi Kharbanda, Dinesh Kumar, Soumik Siddhanta","doi":"10.1021/acs.analchem.5c00183","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00183","url":null,"abstract":"A method to directly predict the number of nucleic acid bases in a single-stranded DNA (ssDNA) or a genomic DNA has been proposed with a combination of Raman spectroscopy and an Artificial Neural Network (ANN) algorithm. In this work, the algorithm was trained by using the Raman spectroscopic signatures from a cohort of 32 ssDNAs. The algorithm could detectpredict the number of bases in an unknown sequence with an <i>R</i>² value of more than 0.83. Chemical mutation using the hydroxylamine method was performed on a ssDNA and also a genomic herring sperm DNA, and the extent was monitored using optical absorbance measurements. The mutation of bases, such as cytosine, can introduce subtle alterations in the DNA structure, potentially leading to significant biological consequences, including neurodegenerative and epigenetic disorders. Also, during the mutation process, the unstable intermediate can undergo further transformation, converting bases such as cytosine to uracil, thus significantly altering the base-pairing properties of the DNA. A one-to-one correspondence was observed between the experimentally and computationally predicted mutated bases in both the single- and double-stranded DNA (dsDNA), thus opening up avenues for the detection of mutations in a diagnostic setup.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"38 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144211538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Multifunctional Electrochemical Biosensor Based on Near-Infrared Light-Responsive Hydrogel for In Vivo Recording and Modulation","authors":"Congyu Zhang, Qi Zheng, Cui Li, Yue Zhu, Jiaqi Ji, Guang Yang, Changman Guo, Yiwei Wang, Qing Zhu, Weizhong Zhu, Dingyi Fu, Shushu Ding","doi":"10.1021/acs.analchem.5c00640","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00640","url":null,"abstract":"Monitoring and regulating neural signals are crucial for the management of neurological disorders. Herein, we constructed a near-infrared light (NIR)-responsive device for controllable recording and modulation of neural signals based on enzyme-loaded microgels/multiwalled carbon nanotubes (MWCNTs) composite-modified microelectrodes. The embedded MWCNTs within the composites can convert NIR light into heat via a photothermal effect. Consequently, on one hand, the enzyme-catalyzed reactions on the electrode surface can be reversibly modulated by NIR light. Under NIR irradiation, the contraction of temperature-responsive microgels not only facilitates direct bioelectrocatalysis but also promotes enzyme cascade catalysis on the electrode surface. The proposed method can determine glucose concentrations ranging from 0.3 to 6.0 mM with a detection limit of 0.098 mM. Taking advantage of the superior antifouling ability and biocompatibility, this device was applied for controllable monitoring of glucose in vivo with excellent stability for up to 2 h. On the other hand, the implanted electrode enabled photothermal activation of TRPV1 channels, which further promoted the autophagy of microglia and the degradation of alpha-synuclein in the mouse brain with Parkinson’s disease. We believe this proposed system, with high spatiotemporal controllability, biocompatibility, and multifunctionality, has great prospects for the diagnostics and therapeutics of neurodegenerative diseases.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"15 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144202381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Drug Monitoring of Amikacin and Colistin in Patients with Multidrug-Resistant Gram-Negative Infections Using a Portable Plasmonic Biosensor","authors":"Alejandro Astúa, Maria Carmen Estevez, Sonia Luque, Santiago Grau, Luisa Sorlí, Milagro Montero, Juan P. Horcajada, Laura M. Lechuga","doi":"10.1021/acs.analchem.4c06748","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c06748","url":null,"abstract":"Innovative diagnostic tools that enhance antibiotic routine monitoring can improve the management of infections caused by antibiotic-resistant bacteria. Therapeutic drug monitoring (TDM) involves measuring drug levels in the patient bloodstream to ensure optimal efficacy and safety, particularly for drugs with a narrow therapeutic index (TI), assisting in dosage control and toxicity risk management. Amikacin (AK) and colistin (CS) are crucial antibiotics for treating multidrug-resistant (MDR) bacteria but they have side effects that require a precise TDM to try to minimize them. Current analytical techniques like immunoassays, high-performance liquid chromatography (HPLC), and liquid chromatography–mass spectrometry (LC-MS) are gold standards for the antibiotic analysis, but they may require transferring the human samples to centralized facilities, delaying crucial results and turnaround time. In contrast, plasmonic biosensors offer advantages for clinical diagnostics, enabling real-time drug detection with minimal sample volume and processing, being ideal for point-of-care applications. We have implemented plasmonic biosensors to quantify and rapidly monitor blood levels of amikacin and colistin. The biosensors provide high specificity and sensitivity, with limits of detection (LOD) of 0.92 ng/mL (1.57 nM) for amikacin and 9.11 pg/mL (7.88 pM) for colistin in blood serum. Statistics analyses demonstrated a strong correlation between the biosensor evaluation and the standard analytical methods (Spearman’s correlation coefficient of 0.9171 (<i>p</i>-value &lt; 0.001) and 0.7435 (<i>p</i>-value = 0.04) for amikacin and colistin, respectively). Our plasmonic biosensors offer in addition, simplicity, portability, and label-free evaluation, with multiplexed capabilities. The rapid turnaround of results in under 20 min, coupled with minimal sample processing, enhances the feasibility of personalized TDM, supporting tailored treatment strategies that can improve patient outcomes. This work lays the foundation for creating an integrated point-of-care biosensor platform for effectively performing TDM of antibiotics and other drugs in real-time at the patient’s bedside in clinical settings.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"62 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144202378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}