Oriented Antibody Functionalized Plasmonic Yolk-Shell-Satellite Nanostructures for Ultrasensitive SERS-Based Lateral Flow Immunoassay.

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2025-07-23 DOI:10.1021/acs.analchem.5c02561

Jiaojiao Sun,Lei Li,Rui Sun,Huancai Yin,Jie Liu

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引用次数: 0

Abstract

Conventional gold nanoparticle (AuNP)-based lateral flow immunoassays (LFIAs) for detecting matrix metalloproteinase-9 (MMP-9), a biomarker associated with dry eye disease and cancer, often suffer from limited sensitivity. To address this, we developed an ultrasensitive surface-enhanced Raman scattering (SERS)-based LFIA using plasmonic yolk-shell-satellite nanostructures (YSSNs) functionalized with bifunctional 4-mercaptophenylboronic acid (MPBA) as SERS tags. The YSSNs, composed of a gold nanorod core enclosed by a gold nanocage and decorated with satellite nanoparticles, provided strong SERS enhancement. MPBA not only acted as a robust Raman reporter but also improved the orientation of antibodies. The SERS-LFIA achieved a limit of detection of 0.0056 ng·mL-1 in PBS, a 305-fold improvement over conventional AuNP-LFIA, and demonstrated favorable linear detection in biological samples, with limits of 0.051 ng·mL-1 in serum and 0.030 ng·mL-1 in tears. This assay offers a sensitive and reliable platform for MMP-9 detection and has great potential for disease diagnostics.

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面向抗体功能化等离子体卵黄-壳-卫星纳米结构的超灵敏sers横向流动免疫分析。

传统的基于金纳米颗粒（AuNP）的横向流动免疫测定（LFIAs）用于检测基质金属蛋白酶-9 (MMP-9)，这是一种与干眼病和癌症相关的生物标志物，通常具有有限的敏感性。为了解决这个问题，我们开发了一种基于表面增强拉曼散射（SERS）的超灵敏LFIA，使用双功能4-巯基苯硼酸（MPBA）功能化的等离子体蛋黄-壳-卫星纳米结构（YSSNs）作为SERS标签。YSSNs由金纳米笼包裹的金纳米棒核心组成，并装饰有卫星纳米粒子，具有很强的SERS增强作用。MPBA不仅作为一个强大的拉曼报告基因，而且还改善了抗体的定向。SERS-LFIA在PBS中的检出限为0.0056 ng·mL-1，比传统AuNP-LFIA提高了305倍，并且在生物样品中表现出良好的线性检测，在血清中的检出限为0.051 ng·mL-1，在眼泪中的检出限为0.030 ng·mL-1。该方法为MMP-9的检测提供了一个灵敏可靠的平台，在疾病诊断方面具有很大的潜力。

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.