Biomacromolecules最新文献_第2页

Lysozyme/Alginate Interactions: Structural and Thermodynamic Insights through ITC and SAXS. 溶菌酶/海藻酸盐相互作用：通过ITC和SAXS的结构和热力学见解。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-07-23 DOI: 10.1021/acs.biomac.5c00270

Asna Vakeri, Antoine Bouchoux, Adeline Boire, Pascaline Hamon, Saïd Bouhallab, Denis Renard

{"title":"Lysozyme/Alginate Interactions: Structural and Thermodynamic Insights through ITC and SAXS.","authors":"Asna Vakeri, Antoine Bouchoux, Adeline Boire, Pascaline Hamon, Saïd Bouhallab, Denis Renard","doi":"10.1021/acs.biomac.5c00270","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00270","url":null,"abstract":"Coacervation and aggregation are distinct phase separation phenomena influenced by molecular properties and physicochemical conditions, such as pH and ionic strength. We investigate lysozyme (LYS)-alginate (ALG) mixtures at pH 7, focusing on the role of ionic strength in determining whether liquid-liquid phase separation (LLPS) or liquid-solid phase separation (LSPS) occurs. Using Isothermal Titration Calorimetry (ITC) and Small-Angle X-ray Scattering (SAXS), we find that a low salt (0-50 mM NaCl) induces compact fractal aggregates, while the intermediate salt (100-150 mM) yields coexisting or pure coacervates composed of larger swollen primary globules. At 200 mM NaCl, soluble complexes form instead of phase separation. ITC data reveal that both LSPS and LLPS are electrostatically driven, with the binding strength decreasing ∼50-fold from LSPS to LLPS conditions. These results demonstrate that phase behavior is tunable via ionic strength and that stronger interactions correlate with denser structures, highlighting distinct structural and energetic signatures for LSPS and LLPS.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermoresponsive Hyaluronate-Based Nanogels for Enhanced Phenanthriplatin Delivery in Cisplatin-Resistant Ovarian Cancer. 基于热反应性透明质酸的纳米凝胶增强顺铂耐药卵巢癌非那西铂的递送。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-07-22 DOI: 10.1021/acs.biomac.5c00692

Filip Latečka, Tamara Juriňáková, Lukáš Münster, Monika Muchová, Michal Masařík, Anton Kuchynski, Petr Humpolíček, Michaela Fojtů, Jan Vícha

{"title":"Thermoresponsive Hyaluronate-Based Nanogels for Enhanced Phenanthriplatin Delivery in Cisplatin-Resistant Ovarian Cancer.","authors":"Filip Latečka, Tamara Juriňáková, Lukáš Münster, Monika Muchová, Michal Masařík, Anton Kuchynski, Petr Humpolíček, Michaela Fojtů, Jan Vícha","doi":"10.1021/acs.biomac.5c00692","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00692","url":null,"abstract":"Stimuli-responsive hyaluronic acid carriers face limitations due to limited carboxyl groups, which are divided between drug conjugation and functional modifications. Thermoresponsive nanogels based on selectively oxidized hyaluronan (2,3-dicarboxy hyaluronate, DCH) grafted with poly(N-isopropyl acrylamide) (pNIPAM) were developed for phenanthriplatin (PhPt) delivery. Sequential oxidation after pNIPAM grafting introduced additional carboxylic groups, enabling a more efficient drug loading and controlled release. Compared to nonoxidized pNIPAM-modified HA, this approach achieved 3 times higher loading efficacy and significantly slower drug release. Upon PhPt loading, DCH-pNIPAM conjugates self-assembled into nanogels, with the drug binding mode (ionic vs covalent) influencing particle rearrangement and drug release behavior. Covalently bound PhPt showed reduced release compared to nonthermoresponsive controls. In vitro studies on ovarian cancer cell lines, including cisplatin-resistant variants, demonstrated up to an 18-fold increase in cytotoxicity versus free PhPt. These nanogels offer a promising strategy for enhancing drug efficacy, reducing off-target effects, and overcoming resistance in cancer therapy.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding and Controlling the Multistep Crystallization of Fatty Acid Cellulose Esters. 脂肪酸纤维素酯多步结晶的认识与控制。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-07-22 DOI: 10.1021/acs.biomac.5c00278

Carina Breuer, Linus Sprandl, Olaf Soltwedel, Regine von Klitzing, Andreas Geissler, Markus Biesalski

{"title":"Understanding and Controlling the Multistep Crystallization of Fatty Acid Cellulose Esters.","authors":"Carina Breuer, Linus Sprandl, Olaf Soltwedel, Regine von Klitzing, Andreas Geissler, Markus Biesalski","doi":"10.1021/acs.biomac.5c00278","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00278","url":null,"abstract":"Fatty acid cellulose esters (FACEs) have great potential as biogenic alternatives to petroleum-based plastics. Nevertheless, their thermal property profiles have been the subject of controversial discussion, particularly with respect to their crystallization behavior. A thorough understanding of the crystallization behavior of FACEs is fundamentally important for gaining deep insights into their solid-state characteristics and thermal properties. This systematic study succeeds in elucidating the crystallization behavior of FACEs as bulk materials. Differential scanning calorimetry combined with rheological analysis and X-ray diffraction of cellulose esters bearing side chains with varying numbers of fatty acid residues (C-12-C-20) and degrees of substitution (ranging from partial to full esterification, i.e., 0.1-3.0) revealed that crystallization of FACEs occurs in multiple stages. These stages involve the distinct crystallization of inter and intramolecular side chains and polysaccharide backbones. A quantitative multistep crystallization mechanism is proposed based on the study findings.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulation of Photoluminescence To Detect Glutathione-Responsiveness of Phthalimide-Derived Polymeric Micelles. 光致发光调制检测邻苯二胺衍生聚合物胶束谷胱甘肽响应性。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-07-22 DOI: 10.1021/acs.biomac.5c00331

Kalipada Manna, Padmapani Pradhan, Subha Samanta, Abhipsa Sekhar Biswal, Santosh Kumar Jana, Sukhendu Mandal, Soumit Chatterjee, Sagar Pal

{"title":"Modulation of Photoluminescence To Detect Glutathione-Responsiveness of Phthalimide-Derived Polymeric Micelles.","authors":"Kalipada Manna, Padmapani Pradhan, Subha Samanta, Abhipsa Sekhar Biswal, Santosh Kumar Jana, Sukhendu Mandal, Soumit Chatterjee, Sagar Pal","doi":"10.1021/acs.biomac.5c00331","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00331","url":null,"abstract":"The delivery of a drug payload to targeted cells using a polymeric vehicle cannot be accurately traced solely by photoluminescence emission. A multicomponent synthetic method is imperative that should integrate fluorescence change observation along with in vitro release of the therapeutic agent. The present work outlines the design, synthesis, spectroscopic characterization, theoretical approaches, GSH-triggered surface morphology observation, and in vitro release of curcumin from a phthalimide-based pPEGMA22-b-pPTHDS16 copolymer micelle. This copolymer has a disulfide bond as a cleavable linker, a phthalimide group as a fluorescent marker, and curcumin as a model cargo. Disulfide cleavage, initiated by interaction with glutathione under physiological conditions, leads to the liberation of free curcumin and a simultaneous red-shift fluorescence emission (λmax = 530 nm). This promising drug delivery system demonstrates potential for theranostic applications for the targeted therapies, as it seamlessly integrates treatment and real-time imaging of drug absorption at a cellular level.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphates Drive the Assembly of Fibers Made from the α-Helical Lentiviral Transduction Enhancer Vectofusin. 磷酸盐驱动由α-螺旋慢病毒转导增强子Vectofusin制成的纤维的组装。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-07-22 DOI: 10.1021/acs.biomac.5c00554

Ahmad Ali Saad, Jian-Qiao Jiang, Jesus Raya, Burkhard Bechinger

{"title":"Phosphates Drive the Assembly of Fibers Made from the α-Helical Lentiviral Transduction Enhancer Vectofusin.","authors":"Ahmad Ali Saad, Jian-Qiao Jiang, Jesus Raya, Burkhard Bechinger","doi":"10.1021/acs.biomac.5c00554","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00554","url":null,"abstract":"Vectofusin (VF) is a histidine-rich amphipathic peptide designed to enhance lentiviral transduction for gene therapeutic applications, where its assembly into fibrils requires polyvalent anions. In this study, we used solid-state NMR, transmission electron microscopy, and titration experiments to investigate the peptide's phosphate-driven supramolecular assembly. A VF variant lacking two lysines (V2K) was used to further assess the role of charge in these assemblies. Our results show that VF-pyrophosphate self-assembles into ordered, raft-like sheet structures. NMR confirmed that VF-pyrophosphate aggregates maintain an α-helical conformation, with distinct phosphate populations, one of which closely interacts with lysine residues. In contrast, the V2K variant showed weaker pyrophosphate interactions, highlighting the importance of electrostatic contacts in assembly. Based on these findings, we propose a model in which phosphates act as electrostatic glue, linking peptides via their lysine side chains. These insights support the design of new self-assembling biomaterials and improve understanding of phosphate-polypeptide interactions in biological processes.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AIEgen-Based Photoactivatable Polymeric Prodrug Nanoplatform for Combined Photodynamic-Chemotherapy. 基于aiegen的光激活聚合前药纳米平台光动力联合化疗。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-07-21 DOI: 10.1021/acs.biomac.5c00763

Xiaoqing Yang, Xuehong Min, Xiaoqing Yi, Xinru Nan, Chendi Qin, Chenghao Liu, Ziyue Gong, Linjian Fang, Shijie Zhen, Man Zhou

{"title":"AIEgen-Based Photoactivatable Polymeric Prodrug Nanoplatform for Combined Photodynamic-Chemotherapy.","authors":"Xiaoqing Yang, Xuehong Min, Xiaoqing Yi, Xinru Nan, Chendi Qin, Chenghao Liu, Ziyue Gong, Linjian Fang, Shijie Zhen, Man Zhou","doi":"10.1021/acs.biomac.5c00763","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00763","url":null,"abstract":"The combination of photodynamic therapy (PDT) and chemotherapy is an innovative cancer treatment strategy, but its clinical application faces challenges: (1) conventional photosensitizers exhibit fluorescence quenching and reduced ROS generation due to aggregation in aqueous environments, and (2) existing nanocarriers suffer from low drug loading efficiency, premature leakage, and systemic toxicity. To overcome these issues, we developed a photoactivatable codelivery system integrating a red-emissive AIEgen photosensitizer (BTPTT-NTPE, BC) with a ROS-responsive polymeric paclitaxel (PTX) prodrug. Harnessing the AIE effect, the AIEgen photosensitizer in its aggregated state within the codelivery system not only resolves the fluorescence quenching issue common to conventional photosensitizers but also demonstrates exceptional photostability and efficient ROS generation. The BC@PTP prodrug achieves a high PTX loading (18.0%) and enables spatiotemporally controlled drug release via light-activated prodrug cleavage. This multifunctional system demonstrates remarkable antitumor efficacy through synergistic PDT-chemotherapy effects in a HeLa-bearing mouse animal model.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Lignin-Based Biodegradable Mulch Film with Zn²⁺ Coordination Networks for Sustainable Agriculture. 具有Zn2+配位网络的木质素基生物可降解地膜的研究。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-07-19 DOI: 10.1021/acs.biomac.5c00607

Jiaqian Cui, Junwei Tang, Xijiao Bian, Jungang Jiang, Yifan Zhang, Lei Wang

{"title":"A Lignin-Based Biodegradable Mulch Film with Zn2+ Coordination Networks for Sustainable Agriculture.","authors":"Jiaqian Cui, Junwei Tang, Xijiao Bian, Jungang Jiang, Yifan Zhang, Lei Wang","doi":"10.1021/acs.biomac.5c00607","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00607","url":null,"abstract":"The environmental persistence of conventional plastic mulch films imposes substantial ecological burdens. With rising demands for sustainable agriculture, biodegradable alternatives attract growing interest. However, existing biobased films often face trade-offs between mechanical robustness, environmental adaptability, and functional versatility. In this study, we fabricated a mechanically enhanced multifunctional lignin-based composite mulch by blending modified sulfate lignin (SKL), poly(vinyl alcohol) (PVA), nanocellulose (CNF), and ZnCl2. The work elucidated the synergistic interfacial mechanism underlying its superior properties. Zn2+ coordination with lignin sulfonic acid groups and SKL/PVA/CNF hydrogen bonding form a hierarchical network. This yields exceptional toughness (40.57 MJ/m3), ∼99% UV-blocking, hydrophobicity, moisture barrier, and thermal stability. The film degraded 70% in 40 days─far exceeding conventional mulch (CM). Its inherent biodegradability offers significant potential to replace agricultural plastics, advancing sustainable materials.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cleavable Cationic Carbosilane Dendrimers with pH-Tunable Charge as siRNA Carriers. 带ph可调电荷的可切割阳离子碳硅烷树状大分子作为siRNA载体。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-07-18 DOI: 10.1021/acs.biomac.5c00344

Judith Recio-Ruiz, Paulina Rycharska, Małgorzata Grygiel, Sylwia Michlewska, Maria Bryszewska, Maksim Ionov, Francisco Javier de la Mata, Sandra García-Gallego

{"title":"Cleavable Cationic Carbosilane Dendrimers with pH-Tunable Charge as siRNA Carriers.","authors":"Judith Recio-Ruiz, Paulina Rycharska, Małgorzata Grygiel, Sylwia Michlewska, Maria Bryszewska, Maksim Ionov, Francisco Javier de la Mata, Sandra García-Gallego","doi":"10.1021/acs.biomac.5c00344","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00344","url":null,"abstract":"Gene therapy is a cutting-edge technique for the prevention or treatment of diseases, which demands the development of biocompatible and efficient vectors. Additionally, for cancer therapy, a precise control on the vectors' response at different pH values is required due to the heterogeneity of the tumors' pH. Many nonviral vectors are based on quaternary ammonium compounds, thus exhibiting potential toxicity and persistence. In this work, we designed a new family of cationic carbosilane dendrimers, which exhibits two differential features: a pH-tunable charge, which can modulate the interaction between the nanocarrier and the siRNA, and a cleavable core, which can further enhance the release of the cargo. We thoroughly explored the structural and biophysical characteristics of the new dendrimers at different pH values: 4.5, 5.5, and 7.4. Additionally, biocompatibility in tumoral and nontumoral cells was evaluated along with the hemolytic effect. Finally, we provided a proof-of-concept for their use as pH-responsive nanocarriers of siRNA.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Elastin-Like Protein for Injectable Tissue-Like Network. 一种可注射组织样网络的弹性蛋白样蛋白。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-07-18 DOI: 10.1021/acs.biomac.5c00536

Yi Yao, Yueyue Kong, You Long, Xiangyu Zhang, Lilong Du, Kai Yang, Xinmeng Liu, Sijia Xu, Lei Zhang, Jing Yang

{"title":"An Elastin-Like Protein for Injectable Tissue-Like Network.","authors":"Yi Yao, Yueyue Kong, You Long, Xiangyu Zhang, Lilong Du, Kai Yang, Xinmeng Liu, Sijia Xu, Lei Zhang, Jing Yang","doi":"10.1021/acs.biomac.5c00536","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00536","url":null,"abstract":"Protein-based tissue fillers are promising for adipose regeneration due to their biocompatibility and tunable properties. Inspired by elastin's structure, we designed a low-immunogenicity \"multiple-domain-collective elastin-like\" (MDCE) protein for injectable tissue-like networks. The MDCE protein was composed of thermal-responsive elastin-like peptides (disordered domains), polyalanine enrichment (functional ordered domains), and zwitterionic polypeptides (hydrophilic domains). This design endows the MDCE protein hydrogel with in situ injectability and antifouling properties, resulting in a tissue-like modulus-tunable filler formation in vivo without chemical cross-linkers and prevention of foreign body rejection. Adipose stem cells, encapsulated in the MDCE hydrogel, can maintain activity for at least ∼9 days (>80% survival rate). In a rat model of adipose tissue defect, a 3-month injection of MDCE hydrogel for efficiently filling adipose tissue presents superior stability and lower immunogenicity compared to the bovine-source elastin proteins. This work provides methods for tissue fillers and scaffolds, aiding medical regeneration, particularly facial rejuvenation.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protometabolically Generated NADH Mediates Material Properties of Aqueous Dispersions to Coacervate Microdroplets. 原代谢产生的NADH介导水分散体的材料特性以凝聚微滴。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-07-18 DOI: 10.1021/acs.biomac.5c00349

Rudrarup Bose, Daniele Rossetto, Anju Tomar, Sanguen Lee, Sheref S Mansy, T-Y Dora Tang

{"title":"Protometabolically Generated NADH Mediates Material Properties of Aqueous Dispersions to Coacervate Microdroplets.","authors":"Rudrarup Bose, Daniele Rossetto, Anju Tomar, Sanguen Lee, Sheref S Mansy, T-Y Dora Tang","doi":"10.1021/acs.biomac.5c00349","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00349","url":null,"abstract":"Macromolecular assembly between biomolecules dictates the material state of chemically complex aqueous dispersions such as the cytoplasm. The formation of protein precipitates, fibers, or liquid droplets have been associated with metabolic regulation and disease. However, the effect of metabolic flux on the material properties of aqueous dispersions remains underexplored. Here, we use the protometabolic reduction of NAD+ to NADH by pyruvate to study the effect of NADH production on the phase separation properties of polyarginine. We show that reduction of NAD+ in the presence of polyarginine can tune the material properties of the dispersion between precipitates, homogeneous solution, and liquid droplets depending on the buffer concentration. In situ droplet formation results in 2-3 times higher reaction rate and NADH yield, compared to homogeneous solution. Our study provides a setting for coupling protometabolism to active protocell environments in the absence of enzymes and sheds light on the self-regulation of metabolic flux on mediating biomolecular phase separation.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0