Biomacromolecules最新文献_第3页

Preparation and Wound Repair of Injectable and Self-Healing Benzaldehyde-Modified Konjac Glucomannan Oligosaccharide/Polyglutamic Acid/ε-Polylysine Hydrogel.

IF 5.5 2区化学

Biomacromolecules Pub Date : 2024-12-12 DOI: 10.1021/acs.biomac.4c01407

Feng Huang, Jiajie Chen, Xuan Tang, Yujian Li, Haixin Bao, Xuan Mao, Shunqing Tang

{"title":"Preparation and Wound Repair of Injectable and Self-Healing Benzaldehyde-Modified Konjac Glucomannan Oligosaccharide/Polyglutamic Acid/ε-Polylysine Hydrogel.","authors":"Feng Huang, Jiajie Chen, Xuan Tang, Yujian Li, Haixin Bao, Xuan Mao, Shunqing Tang","doi":"10.1021/acs.biomac.4c01407","DOIUrl":"https://doi.org/10.1021/acs.biomac.4c01407","url":null,"abstract":"Oligosaccharides always have better water solubility, higher possibilities for modification, and unique biofunctions compared with polysaccharides, but they are rarely used as the matrix of a hydrogel. Here, we prepared a composite BKOS/HPGA/PL hydrogel (BKPP hydrogel) constructed by hydrazone/imine bonds between the aldehyde groups of benzaldehyde-modified konjac glucomannan oligosaccharide (BKOS) and the primary amino groups of both hydrazide-modified polyglutamic acid (HPGA) and ε-polylysine (ε-PL). The hydrogels had both injectable and self-healing properties. The gelation time reached 23 s when 2% of BKOS (DS = 21.7%), 10% HPGA (DS = 11.5%), and 10% ε-PL solutions were mixed in a volume ratio of 5:4.5:0.5. Besides high water-retention capability and good cytocompatibility, the hydrogel also maintained both the immunoactivities of BKOS and the antibacterial performances of ε-PL and HPGA, and thus exhibited good wound healing performance in the whole cortex wound repair process of rats, which might have potential for its biomedical application.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discrimination between Purine and Pyrimidine-Rich RNA in Liquid-Liquid Phase-Separated Condensates with Cationic Peptides and the Effect of Artificial Crowding Agents.

IF 5.5 2区化学

Biomacromolecules Pub Date : 2024-12-11 DOI: 10.1021/acs.biomac.4c01282

Anika L Moller, Isis A Middleton, Grace E Maynard, Lachlan B Cox, Anna Wang, Hsiu L Li, Pall Thordarson

{"title":"Discrimination between Purine and Pyrimidine-Rich RNA in Liquid-Liquid Phase-Separated Condensates with Cationic Peptides and the Effect of Artificial Crowding Agents.","authors":"Anika L Moller, Isis A Middleton, Grace E Maynard, Lachlan B Cox, Anna Wang, Hsiu L Li, Pall Thordarson","doi":"10.1021/acs.biomac.4c01282","DOIUrl":"https://doi.org/10.1021/acs.biomac.4c01282","url":null,"abstract":"Membraneless organelles, often referred to as condensates or coacervates, are liquid-liquid phase-separated systems formed between noncoding RNAs and intrinsically disordered proteins. While the importance of different amino acid residues in short peptide-based condensates has been investigated, the role of the individual nucleobases or the type of heterocyclic structures, the purine vs pyrimidine nucleobases, is less researched. The cell's crowded environment has been mimicked in vitro to demonstrate its ability to induce the formation of condensates, but more research in this area is required, especially with respect to RNA-facilitated phase separation and the properties of the crowding agent, poly(ethylene glycol) (PEG). Herein, we have shown that the nucleotide base sequence of RNA can greatly influence its propensity to undergo phase separation with cationic peptides, with the purine-only RNA decamer (AG)5 readily doing so while the pyrimidine-only (CU)5 does not. Furthermore, we show that the presence and size of a PEG macromolecular crowder affects both the ability to phase separate and the stability of coacervates formed, possibly due to co-condensation of PEG with the RNA and peptides. This work sheds light on the presence of low-complexity long purine- or pyrimidine-rich noncomplementary repeat (AG or CU) sequences in various noncoding RNAs found in biology.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Whole Is Greater than the Sum of Its Parts - Challenges and Perspectives in Polyelectrolytes.

IF 5.5 2区化学

Biomacromolecules Pub Date : 2024-12-11 DOI: 10.1021/acs.biomac.4c01061

Anja Traeger, Meike N Leiske

{"title":"The Whole Is Greater than the Sum of Its Parts - Challenges and Perspectives in Polyelectrolytes.","authors":"Anja Traeger, Meike N Leiske","doi":"10.1021/acs.biomac.4c01061","DOIUrl":"https://doi.org/10.1021/acs.biomac.4c01061","url":null,"abstract":"Polyelectrolytes offer unique properties for biological applications due to their charged nature and high water solubility. Here, the challenges in their synthesis and characterization techniques are reviewed, emphasizing that their strong interactions with the surrounding media and counterions must be considered when working with this interesting class of materials. Their potential in complexation for gene delivery, their unique stealth and anti-fouling properties, and their more specific interactions with amino acid transporters for cancer therapy are highlighted. The underlying mechanisms responsible for their biological efficacy, including the proton sponge effect for endosomal release and their interactions with cellular membranes, are addressed. For polyelectrolytes with a high level of usage, an overview is given of their historical context. This Perspective outlines the potential of polyelectrolytes for innovative applications in the field of biomedicine. Considering the physicochemical characteristics of this class of materials, this work strives to elucidate the distinctive properties and applications of polyelectrolytes.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological Condensate Growth: Examining the Impact of Solute Crowder on Size Expansion.

IF 5.5 2区化学

Biomacromolecules Pub Date : 2024-12-10 DOI: 10.1021/acs.biomac.4c01152

Shangqiang Xie, Congran Yue, Sheng Ye, Zhenlu Li

引用次数: 0

Ion-Mediated Cross-Linking of Hyaluronic Acid into Hydrogels without Chemical Modification. 离子介导透明质酸交联成水凝胶而无需化学修饰

IF 5.5 2区化学

Biomacromolecules Pub Date : 2024-12-09 Epub Date: 2024-11-01 DOI: 10.1021/acs.biomac.4c00985

Gayatri Prakash, Aaron J Clasky, Kunal Gadani, Mohammad Nazeri, Frank X Gu

引用次数: 0

Harnessing Dual-Responsive Polymeric Micelles for Precision Oxidative Stress Amplification in Targeted Cancer Therapy.

IF 5.5 2区化学

Biomacromolecules Pub Date : 2024-12-09 DOI: 10.1021/acs.biomac.4c01250

Manseok Yang, Sujin Kim, Seungwon Jeong, Suyeon Lee, Seunga Lee, Hanui Jo, Nuri Kim, Nanhee Song, Seong-Cheol Park, Dongwon Lee

{"title":"Harnessing Dual-Responsive Polymeric Micelles for Precision Oxidative Stress Amplification in Targeted Cancer Therapy.","authors":"Manseok Yang, Sujin Kim, Seungwon Jeong, Suyeon Lee, Seunga Lee, Hanui Jo, Nuri Kim, Nanhee Song, Seong-Cheol Park, Dongwon Lee","doi":"10.1021/acs.biomac.4c01250","DOIUrl":"https://doi.org/10.1021/acs.biomac.4c01250","url":null,"abstract":"Targeting the altered redox balance in cancer cells, this study explores a strategy to induce selective cancer cell death by combining reactive oxygen species (ROS) generation with glutathione (GSH) depletion. We developed oxidative stress-amplifying polymeric (pCB) micelles that function both as therapeutic agents and carriers for GSH-depleting retinoic acid prodrug (BRDP). pCB incorporating ROS-generating cinnamaldehyde and a GSH-depleting quinone methide precursor could self-assemble into micelles encapsulating BRDP, delivering both ROS generators and GSH-depleting drugs. The micelles were surface-functionalized with the tripeptide Arg-Gly-Asp (RGD) for targeted delivery to integrin-overexpressing tumors. In a mouse xenograft model, RGD-decorated BRDP-loaded micelles significantly accumulated in tumor sites, enhancing anticancer efficacy without toxicity to normal tissues. This study marks significant advancement in the field of oxidative stress-amplifying polymeric precursors, presenting a novel and highly effective anticancer therapeutic approach that integrates multiple tumor-specific triggers and ROS-mediated mechanisms.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coarse-Grain Model of Ultrarigid Polymer Rods Comprising Bifunctionally Linked Peptide Bundlemers. 包含双功能连接肽束的超硬聚合物棒的粗粒模型。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2024-12-09 Epub Date: 2024-11-05 DOI: 10.1021/acs.biomac.4c01192

Tianren Zhang, Dai-Bei Yang, Christopher J Kloxin, Darrin J Pochan, Jeffery G Saven

引用次数: 0

Retraction of "Solubilization of Docetaxel in Poly(ethylene oxide)-block-poly(butylene/styrene oxide) Micelles".

IF 5.5 2区化学

Biomacromolecules Pub Date : 2024-12-09 DOI: 10.1021/acs.biomac.4c01583

Mahmoud Elsabahy, Marie-Ève Perron, Nicolas Bertrand, Ga-Er Yu, Jean-Christophe Leroux

引用次数: 0

Antimicrobial Macrocycles - Synthesis, Characterization, and Activity Comparison with Their Linear Polycationic Analogues. 抗菌大环 - 与线性多阳离子类似物的合成、表征和活性比较。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2024-12-09 Epub Date: 2024-11-08 DOI: 10.1021/acs.biomac.4c01099

Rafał Jerzy Kopiasz, Maciej Dranka, Waldemar Tomaszewski, Patrycja Kowalska, Beata Butruk-Raszeja, Karolina Drężek, Jolanta Mierzejewska, Tomasz Ciach, Dominik Jańczewski

{"title":"Antimicrobial Macrocycles - Synthesis, Characterization, and Activity Comparison with Their Linear Polycationic Analogues.","authors":"Rafał Jerzy Kopiasz, Maciej Dranka, Waldemar Tomaszewski, Patrycja Kowalska, Beata Butruk-Raszeja, Karolina Drężek, Jolanta Mierzejewska, Tomasz Ciach, Dominik Jańczewski","doi":"10.1021/acs.biomac.4c01099","DOIUrl":"10.1021/acs.biomac.4c01099","url":null,"abstract":"One of the promising candidates for new antimicrobial agents is membrane-lytic compounds that kill microbes through cell membrane permeabilization, such as antimicrobial peptides (AMPs) and their synthetic mimics (SMAMPs). Although SMAMPs have been under investigation for nearly 30 years, a few challenges must be addressed before they can reach clinical use. In this work, a step-growth polymerization leading to already-known highly antimicrobial ionenes was redirected toward the formation of macrocyclic quaternary ammonium salts (MQAs) employing a high dilution principle. Antimicrobial assays and cytotoxicity studies revealed the high antimicrobial activity of MQAs and better selectivity than their polymeric analogues. Therefore, MQAs seem to be a new class of promising antibacterial agents. Additionally, membrane-lytic experiments using large unilamellar liposomes (LUVs) and whole cells revealed significant differences between MQAs and ionenes in their ability to adsorb onto the surface of LUVs and microbes as well as their ability to permeate the lipid bilayer.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":"7814-7827"},"PeriodicalIF":5.5,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering an Ultrasound-Responsive Glycopolymersome for Hepatocyte-Specific Gene Delivery. 为肝细胞特异性基因递送设计超声响应性聚糖聚合体

IF 5.5 2区化学

Biomacromolecules Pub Date : 2024-12-09 Epub Date: 2024-11-08 DOI: 10.1021/acs.biomac.4c01128

Ping Wei, Kai Chen, Jinghua Chen

{"title":"Engineering an Ultrasound-Responsive Glycopolymersome for Hepatocyte-Specific Gene Delivery.","authors":"Ping Wei, Kai Chen, Jinghua Chen","doi":"10.1021/acs.biomac.4c01128","DOIUrl":"10.1021/acs.biomac.4c01128","url":null,"abstract":"The ability to design liver-targeted gene delivery vectors is plagued with difficulties ranging from carrier-mediated cellular toxicity to challenges in encapsulating sensitive nucleic acids. Herein, we present an ultrasound-responsive glycopolymersome strategy for in situ loading of nucleic acids and achieving hepatocyte-specific gene delivery. This glycopolymersome is self-assembled from a block copolymer, N-acetylgalactosamine-grafted poly(glutamic acid)-block-poly(ε-caprolactone) (PGAGalNAc-b-PCL). GalNAc is introduced to afford liver targeting through the selective binding to the asialoglycoprotein receptor overexpressed on hepatocytes. External ultrasound is utilized to assist in encapsulating nucleic acids within the hydrophilic lumen of glycopolymersomes by exploiting their ultrasound responsiveness nature. Biological studies confirmed the successful encapsulation of plasmid DNA (pDNA) and small interfering RNA (siRNA), rapid nuclear internalization, and efficient gene transfection. These findings collectively demonstrated that this ultrasound-responsive glycopolymersome could be exploited as a novel safe and efficient gene vector targeting hepatocytes.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":"7838-7849"},"PeriodicalIF":5.5,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0