{"title":"抗血管生成柞蚕丝素纳米载体作为肺肿瘤抑制的先进双基因传递平台。","authors":"Jing Qu, Xueping Liu, Jiru Jia, Wei Ma, Qi Xiao, Mingzhong Li","doi":"10.1021/acs.biomac.5c01484","DOIUrl":null,"url":null,"abstract":"<p><p>The cationic <i>Antheraea pernyi</i> silk fibroin (CASF)-bridged endostatin peptide 2 (ES2) electrostatically coated the inhibitor of growth 4 (ING4)/interleukin-24 (IL-24) dual-gene coexpression plasmid DNA (pDNA) to construct a CASF-ES2/pDNA nanocarrier, which significantly enhanced gene expression and induced apoptosis by strengthening cell adhesion and endocytosis. The continuously released ES2 contributed to its specific action on HUVECs, including inducing apoptosis and hindering migration, invasion, and tube formation, as well as inhibiting angiogenesis in the chorioallantoic membrane. In the treatment of mice with A549 xenograft tumors, CASF-ES2/pDNA arrested the growth cycle of tumor cells and activated the Bcl-2/Bax/C Caspase-3 apoptotic pathway, promoting cell apoptosis and tissue collapse. Additionally, the released ES2 prevented the formation of tumor neovasculature, cut off the nutrient channels for tumor growth, and synergistically inhibited the development and metastasis of xenograft tumors. This safe and antiangiogenic gene vector shows great promise for application in long-term antitumor and antimetastatic therapy.</p>","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.4000,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antiangiogenic <i>Antheraea pernyi</i> Silk Fibroin Nanocarrier As an Advanced Dual-Gene Delivery Platform for Lung Tumor Suppression.\",\"authors\":\"Jing Qu, Xueping Liu, Jiru Jia, Wei Ma, Qi Xiao, Mingzhong Li\",\"doi\":\"10.1021/acs.biomac.5c01484\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The cationic <i>Antheraea pernyi</i> silk fibroin (CASF)-bridged endostatin peptide 2 (ES2) electrostatically coated the inhibitor of growth 4 (ING4)/interleukin-24 (IL-24) dual-gene coexpression plasmid DNA (pDNA) to construct a CASF-ES2/pDNA nanocarrier, which significantly enhanced gene expression and induced apoptosis by strengthening cell adhesion and endocytosis. The continuously released ES2 contributed to its specific action on HUVECs, including inducing apoptosis and hindering migration, invasion, and tube formation, as well as inhibiting angiogenesis in the chorioallantoic membrane. In the treatment of mice with A549 xenograft tumors, CASF-ES2/pDNA arrested the growth cycle of tumor cells and activated the Bcl-2/Bax/C Caspase-3 apoptotic pathway, promoting cell apoptosis and tissue collapse. Additionally, the released ES2 prevented the formation of tumor neovasculature, cut off the nutrient channels for tumor growth, and synergistically inhibited the development and metastasis of xenograft tumors. This safe and antiangiogenic gene vector shows great promise for application in long-term antitumor and antimetastatic therapy.</p>\",\"PeriodicalId\":30,\"journal\":{\"name\":\"Biomacromolecules\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":5.4000,\"publicationDate\":\"2025-10-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomacromolecules\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1021/acs.biomac.5c01484\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomacromolecules","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acs.biomac.5c01484","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Antiangiogenic Antheraea pernyi Silk Fibroin Nanocarrier As an Advanced Dual-Gene Delivery Platform for Lung Tumor Suppression.
The cationic Antheraea pernyi silk fibroin (CASF)-bridged endostatin peptide 2 (ES2) electrostatically coated the inhibitor of growth 4 (ING4)/interleukin-24 (IL-24) dual-gene coexpression plasmid DNA (pDNA) to construct a CASF-ES2/pDNA nanocarrier, which significantly enhanced gene expression and induced apoptosis by strengthening cell adhesion and endocytosis. The continuously released ES2 contributed to its specific action on HUVECs, including inducing apoptosis and hindering migration, invasion, and tube formation, as well as inhibiting angiogenesis in the chorioallantoic membrane. In the treatment of mice with A549 xenograft tumors, CASF-ES2/pDNA arrested the growth cycle of tumor cells and activated the Bcl-2/Bax/C Caspase-3 apoptotic pathway, promoting cell apoptosis and tissue collapse. Additionally, the released ES2 prevented the formation of tumor neovasculature, cut off the nutrient channels for tumor growth, and synergistically inhibited the development and metastasis of xenograft tumors. This safe and antiangiogenic gene vector shows great promise for application in long-term antitumor and antimetastatic therapy.
期刊介绍：
Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine.
Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.
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