Biomacromolecules最新文献_第4页

Viscoelastic Behavior of Aqueous Hydroxypropyl Cellulose Solutions Due to Entanglements. 羟丙基纤维素水溶液因缠结而产生的粘弹性行为。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-01-15 DOI: 10.1021/acs.biomac.4c01646

Misato Yoshida, Hyota Hozumi, Yoshiki Horikawa, Toshiyuki Shikata

{"title":"Viscoelastic Behavior of Aqueous Hydroxypropyl Cellulose Solutions Due to Entanglements.","authors":"Misato Yoshida, Hyota Hozumi, Yoshiki Horikawa, Toshiyuki Shikata","doi":"10.1021/acs.biomac.4c01646","DOIUrl":"https://doi.org/10.1021/acs.biomac.4c01646","url":null,"abstract":"Hydroxypropyl cellulose (HpC) forms a liquid crystalline phase and is thought to have a rod-like shape in aqueous solution. The viscoelastic behaviors of aqueous solutions of HpC samples with average molar substitution numbers (MS ∼ 3.8) and weight-average molar masses (Mw = 36-740 kg mol-1) were examined over a wide concentration (c) range, and the results were discussed based on a concept of rod particle suspension rheology. The c and Mw dependencies of viscoelastic parameters determined in the frequency range showing flow behavior, such as the zero-shear viscosity, the average relaxation time, and the steady-state compliance in the c range for HpC molecules to fully entangle, were considered by using the number density (ν = cNA/Mw, where NA is the Avogadro constant), the intrinsic viscosity, and the average rod particle length of HpC molecules determined via dilute solution properties. The obtained relationships were successfully understood with the rod particle suspension rheology.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142996239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Dynamics Study of Protein-Mediated Electroporation of Kv Channels Induced by nsPEFs: Advantages of Bipolar Pulses. nsPEFs诱导Kv通道蛋白介导电穿孔的分子动力学研究：双极脉冲的优势。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-01-14 DOI: 10.1021/acs.biomac.4c01321

Fei Guo, Jun Xiang, Yue Zhuo, Kai Pei

引用次数: 0

Insights into the Mechanism of Protein Loading by Chain-Length Asymmetric Complex Coacervates. 链长不对称复合体凝聚体蛋白质装载机制的深入研究。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-01-14 DOI: 10.1021/acs.biomac.4c01516

Eugenia Apuzzo, Marilina Cathcarth, Agustín S Picco, Catalina von Bilderling, Omar Azzaroni, Maximiliano L Agazzi, Santiago E Herrera

{"title":"Insights into the Mechanism of Protein Loading by Chain-Length Asymmetric Complex Coacervates.","authors":"Eugenia Apuzzo, Marilina Cathcarth, Agustín S Picco, Catalina von Bilderling, Omar Azzaroni, Maximiliano L Agazzi, Santiago E Herrera","doi":"10.1021/acs.biomac.4c01516","DOIUrl":"https://doi.org/10.1021/acs.biomac.4c01516","url":null,"abstract":"The study of the phase behavior of polyelectrolyte complex coacervates has attracted significant attention in recent years due to their potential use as membrane-less organelles, microreactors, and drug delivery platforms. In this work, we investigate the mechanism of protein loading in chain-length asymmetric complex coacervates composed of a polyelectrolyte and an oppositely charged multivalent ion. Unlike the symmetric case (polycation + polyanion), we show that protein loading is highly selective based on the protein's net charge: only proteins with charges opposite to the polyelectrolyte can be loaded. Through a series of systematic experiments, we identified that the protein loading process relies on the formation of a neutral three-component coacervate in which both the protein and the multivalent ion serve as complexing agents for the polyelectrolyte. Lastly, we demonstrated that this mechanism extends to the sequestration of other charged small molecules, offering valuable insights into designing functional multicomponent coacervates.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic Effect of Polyglycerol and DMSO for Long-Term Cryopreservation of Stichococcus Species. 聚甘油和二甲基亚砜对蜱虫长期低温保存的协同作用。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-01-13 Epub Date: 2024-12-06 DOI: 10.1021/acs.biomac.4c01462

Aram Shin, Seong Ryeol Choi, Jun Ho Yim, Eu Jin Chung, Seung Won Nam, Brendan P Burns, Young Jae Jeon, Byeong-Su Kim

{"title":"Synergistic Effect of Polyglycerol and DMSO for Long-Term Cryopreservation of Stichococcus Species.","authors":"Aram Shin, Seong Ryeol Choi, Jun Ho Yim, Eu Jin Chung, Seung Won Nam, Brendan P Burns, Young Jae Jeon, Byeong-Su Kim","doi":"10.1021/acs.biomac.4c01462","DOIUrl":"10.1021/acs.biomac.4c01462","url":null,"abstract":"Herein, we present a significant advancement in long-term cryopreservation techniques for microalgae Stichococcus species using a combination of linear polyglycerol (linPG) and dimethyl sulfoxide (DMSO). The technique was tested on three Stichococcus species: Stichococcus bacillaris, Stichococcus deasonii, and Stichococcus minor, which showed long-term viability and recovery rates superior to those when treated with a traditional cryoprotectant only. While DMSO alone enabled high cell recovery rates for all species after 1 week of cryopreservation, the rates for some of them dropped below 50% after 26 weeks of cryopreservation. Treating the cells with a combination of linPG and DMSO raised the recovery rates for all three Stichococcus species to above 92% after long-term cryopreservation. Our findings indicate that linPG in combination with DMSO offers a synergistic and effective solution for maintaining cell integrity and functionality during long-term cryopreservation of microalgae.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":"635-643"},"PeriodicalIF":5.5,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioresponsive Polymeric Nanoparticles: From Design, Targeted Therapy to Cancer Immunotherapy. 生物反应性聚合物纳米颗粒：从设计、靶向治疗到癌症免疫治疗。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-01-13 Epub Date: 2024-12-12 DOI: 10.1021/acs.biomac.4c01257

Huanli Sun, Zhiyuan Zhong

{"title":"Bioresponsive Polymeric Nanoparticles: From Design, Targeted Therapy to Cancer Immunotherapy.","authors":"Huanli Sun, Zhiyuan Zhong","doi":"10.1021/acs.biomac.4c01257","DOIUrl":"10.1021/acs.biomac.4c01257","url":null,"abstract":"Bioresponsive polymeric nanoparticles (NPs) that are capable of delivering and releasing therapeutics and biotherapeutics to target sites have attracted vivid interest in cancer therapy and immunotherapy. In contrast to enthusiastic evolution in the academic world, the clinical translation of these smart systems is scarce, partly due to concerns about safety, stability, complexity, and scalability. The moderate targetability, responsivity, and benefits are other concerns. In the past 17 years, we have devoted ourselves to exploring elegant strategies to address the above basic and translational problems by introducing diverse functional groups and/or targeting ligands to safe biomedical materials, such as biodegradable polymers and water-soluble polymers. This minimal modification is critical for further clinical translation. We have tailor-made various bioresponsive NPs including shell-sheddable and/or acid-sensitive biodegradable NPs, disulfide-cross-linked biodegradable micelles and polymersomes, and blood-brain barrier (BBB)-permeable NPs, to target different tumors. This perspective provides an overview of our work path toward targeted nanomedicines and personalized vaccines, which might inspire clinical translation and future research on cancer therapy.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":"33-42"},"PeriodicalIF":5.5,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing Dual-Responsive Polymeric Micelles for Precision Oxidative Stress Amplification in Targeted Cancer Therapy. 利用双响应聚合物胶束精确氧化应激扩增靶向癌症治疗。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-01-13 Epub Date: 2024-12-09 DOI: 10.1021/acs.biomac.4c01250

Manseok Yang, Sujin Kim, Seungwon Jeong, Suyeon Lee, Seunga Lee, Hanui Jo, Nuri Kim, Nanhee Song, Seong-Cheol Park, Dongwon Lee

{"title":"Harnessing Dual-Responsive Polymeric Micelles for Precision Oxidative Stress Amplification in Targeted Cancer Therapy.","authors":"Manseok Yang, Sujin Kim, Seungwon Jeong, Suyeon Lee, Seunga Lee, Hanui Jo, Nuri Kim, Nanhee Song, Seong-Cheol Park, Dongwon Lee","doi":"10.1021/acs.biomac.4c01250","DOIUrl":"10.1021/acs.biomac.4c01250","url":null,"abstract":"Targeting the altered redox balance in cancer cells, this study explores a strategy to induce selective cancer cell death by combining reactive oxygen species (ROS) generation with glutathione (GSH) depletion. We developed oxidative stress-amplifying polymeric (pCB) micelles that function both as therapeutic agents and carriers for GSH-depleting retinoic acid prodrug (BRDP). pCB incorporating ROS-generating cinnamaldehyde and a GSH-depleting quinone methide precursor could self-assemble into micelles encapsulating BRDP, delivering both ROS generators and GSH-depleting drugs. The micelles were surface-functionalized with the tripeptide Arg-Gly-Asp (RGD) for targeted delivery to integrin-overexpressing tumors. In a mouse xenograft model, RGD-decorated BRDP-loaded micelles significantly accumulated in tumor sites, enhancing anticancer efficacy without toxicity to normal tissues. This study marks significant advancement in the field of oxidative stress-amplifying polymeric precursors, presenting a novel and highly effective anticancer therapeutic approach that integrates multiple tumor-specific triggers and ROS-mediated mechanisms.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":"437-448"},"PeriodicalIF":5.5,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of an Injectable Thermosensitive Hydrogel Drug Delivery System for Degenerated Intervertebral Disc Regeneration. 可注射热敏水凝胶给药系统在退变椎间盘再生中的应用。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-01-13 Epub Date: 2024-12-13 DOI: 10.1021/acs.biomac.4c00965

Yuheng Liu, Chuan Guo, Yu Wang, Qing-Quan Kong

引用次数: 0

Impact of Acetylation, Succinylation, and pH on DNA Packaging in PEI-Based Polyplexes. 乙酰化、琥珀酰化和pH对pei基多聚物中DNA包装的影响。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-01-13 Epub Date: 2024-12-17 DOI: 10.1021/acs.biomac.4c00648

Md Nasir Uddin, Md Abu Monsur Dinar, Leah E Schrass, Daniel W Pack, Jason E DeRouchey

引用次数: 0

Flexible and Robust Piezoelectric Chitosan Films with Enhanced Bioactivity. 具有增强生物活性的柔韧而坚固的壳聚糖压电薄膜

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-01-13 DOI: 10.1021/acs.biomac.4c01464

Srishti Chakraborty, Souvik Debnath, Kailas Mahipal Malappuram, Sampath Parasuram, Huan-Tsung Chang, Kaushik Chatterjee, Amit Nain

引用次数: 0

Revealing the Solution Conformation and Hydration Structure of Type I Tropocollagen Using X-ray Scattering and Molecular Dynamics Simulation. 利用x射线散射和分子动力学模拟揭示I型胶原的溶液构象和水合结构。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-01-13 Epub Date: 2025-01-02 DOI: 10.1021/acs.biomac.4c01261

Ying-Jen Shiu, Bradley W Mansel, Kuei-Fen Liao, Ting-Wei Hsu, Je-Wei Chang, Orion Shih, Yi-Qi Yeh, Johannes Allwang, U-Ser Jeng

{"title":"Revealing the Solution Conformation and Hydration Structure of Type I Tropocollagen Using X-ray Scattering and Molecular Dynamics Simulation.","authors":"Ying-Jen Shiu, Bradley W Mansel, Kuei-Fen Liao, Ting-Wei Hsu, Je-Wei Chang, Orion Shih, Yi-Qi Yeh, Johannes Allwang, U-Ser Jeng","doi":"10.1021/acs.biomac.4c01261","DOIUrl":"10.1021/acs.biomac.4c01261","url":null,"abstract":"Hydration plays a crucial role in regulating the dispersion behavior of biomolecules in water, particularly in how pH-sensitive hydration water network forms around proteins. This study explores the conformation and hydration structure of Type-I tropocollagen using small- and wide-angle X-ray scattering (SWAXS) and molecular dynamics (MD) simulations. The results reveal that tropocollagen exhibits a significant softening conformation in solution, transitioning from its rod-like structure in tissues to a worm-like conformation, characterized by a reduced radius of gyration of 50 nm and a persistent length of 34 nm. The SWAXS-supported MD calculations further establish a hydration water network characterized by a 2.8 Å free-water exclusion zone where water molecules are largely hydrogen-bonded to the densely distributed polar groups on the tropocollagen surfaces. These first-layer water molecules are bridged by outer water molecules extending up to 4 Å from the protein surfaces, forming a major hydration shell that encapsulates the protein.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":"449-458"},"PeriodicalIF":5.5,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11734691/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142918650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0