Small Methods最新文献

Manipulation of Wnt/β-Catenin Signaling by Synthetic Frizzled Agonist and LRP Antagonist in Organoid Cultures and In Vivo.

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-03-28 DOI: 10.1002/smtd.202500425

Quanhui Dai, Jiawen Wang, Zihuan Lin, Danni Yu, Hui Yang, Jinsong Wei, Xiaoyu Li, Hao Hu, Chao Ni, Bing Zhao

{"title":"Manipulation of Wnt/β-Catenin Signaling by Synthetic Frizzled Agonist and LRP Antagonist in Organoid Cultures and In Vivo.","authors":"Quanhui Dai, Jiawen Wang, Zihuan Lin, Danni Yu, Hui Yang, Jinsong Wei, Xiaoyu Li, Hao Hu, Chao Ni, Bing Zhao","doi":"10.1002/smtd.202500425","DOIUrl":"https://doi.org/10.1002/smtd.202500425","url":null,"abstract":"Wnt/β-catenin signaling and its dysregulation play critical roles in stem cell fate determination and the pathology of various diseases. However, the application of translated Wnt ligand in regenerative medicine is hampered by its hydrophobicity and cross-reactivity with Frizzled (FZD) receptors. Here, a synthetic key receptor modulator, the FZD agonist RRP-pbFn is generated, for high-efficiency Wnt/β-catenin signaling activation in the absence of direct binding to LRP5/6. RRP-pbFn demonstrates superior potency compared to surrogate Wnt, supporting the growth of diverse mouse and human organoids and inducing the expansion of liver and intestine progenitors in vivo. Complementing this, a synthetic LRP antagonist, RRP-Dkk1c is developed, which exhibits heightened effectiveness in attenuating Wnt/β-catenin signaling activity compared to Dkk1, thereby abolishing the formation of CT26-derived colon cancer xenograft in vivo. Together, these two paired key receptor modulators targeting individual type of cell-surface receptors hold great promise for biomedical research and potential therapeutics.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500425"},"PeriodicalIF":10.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rational Design and Characterization of 2D Metal-Semiconductor Junctions for Optoelectronics by Combining Quantum Transport and Excited State Carrier Dynamics Simulations: Case of 2H-WTe₂ Based Junctions.

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-03-27 DOI: 10.1002/smtd.202402037

Chengfeng Pan, Dazhong Sun, Wang Gong, Leran Xu, Xiuyun Zhang, Anqi Shi, Yongtao Li, Xianghong Niu

{"title":"Rational Design and Characterization of 2D Metal-Semiconductor Junctions for Optoelectronics by Combining Quantum Transport and Excited State Carrier Dynamics Simulations: Case of 2H-WTe2 Based Junctions.","authors":"Chengfeng Pan, Dazhong Sun, Wang Gong, Leran Xu, Xiuyun Zhang, Anqi Shi, Yongtao Li, Xianghong Niu","doi":"10.1002/smtd.202402037","DOIUrl":"https://doi.org/10.1002/smtd.202402037","url":null,"abstract":"Rising 2D metal-semiconductor junctions (MSJs) greatly advance nanoelectronics. Currently, evaluating the performance of MSJs mainly relies on determining the formation of Ohmic or Schottky contacts through static electronic structures. However, when MSJs are integrated into optoelectronic devices, dynamic transport and excited state carrier dynamics become crucial, yet the impact of the interface remains unclear. Herein, taking 2H-WTe2 based vertical and lateral MSJs as examples, their performances are evaluated in the perspective of ground and excited states. Faced with the significant band hybridization induced by strong interfacial coupling, identifying Ohmic contact only by analyzing electronic structures possibly becomes defunct. Through quantum transport simulation, the junction with the largest on-state current can be further filtered. Meanwhile, non-adiabatic molecular dynamics simulation uncovers the carrier lifetime can be shortened or extended as the interfaces are formed, which depends on the competitive relationship between electron-phonon coupling and trap states. In these MSJs studied, the photoexcited carriers with the nanosecond lifetime can be rapidly conducted away by electrodes, indicating a high-effect utilization rate. The work advances the all-around routine for rational designing and characterizing 2D MSJs.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2402037"},"PeriodicalIF":10.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Defect Engineered Few Layered MoS₂ for Human-Machine Interface.

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-03-27 DOI: 10.1002/smtd.202500068

Raksha D Salian, Subhendu Mishra, Chinmayee Chowde Gowda, Ranjan Kumar Barik, Abhishek Kumar Singh, Chandra Sekhar Tiwary, Partha Kumbhakar

{"title":"Defect Engineered Few Layered MoS2 for Human-Machine Interface.","authors":"Raksha D Salian, Subhendu Mishra, Chinmayee Chowde Gowda, Ranjan Kumar Barik, Abhishek Kumar Singh, Chandra Sekhar Tiwary, Partha Kumbhakar","doi":"10.1002/smtd.202500068","DOIUrl":"https://doi.org/10.1002/smtd.202500068","url":null,"abstract":"Ultrasensitive flexible devices have huge applications in many areas, like healthcare monitoring, human-machine interaction, and wearable technology. However, improving the sensitivity of these devices is still challenging. In the current study, a flexible non-contact sensing system is designed with a human-machine interface using defect-engineered, few-layered Molybdenum disulfide (MoS2). The fabricated sensors show high sensitivity when monitoring proximity, humidity, and in-plane applied strain. The output performance demonstrates the influence of surface defects, which greatly impact the average surface charge of the nanosheets. The experimental measurements and in-detail density functional theoretical (DFT) calculation further reveal surface charge variations on the basal planes that correlate with topographic defects and increase sensitivity. The electrical signals for different gestures of human hands are used to illustrate the identification of multidirectional bending and sliding events. These findings will contribute to understanding the effect of surface defects that play an important role in sensing applications with human-machine interface.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500068"},"PeriodicalIF":10.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA Nanoflower-Powered CRISPR/Cas12a Biosensing Platform for Ultrasensitive Protein Detection in Clinical Samples.

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-03-27 DOI: 10.1002/smtd.202402130

Chen Ji, Yongming Han, Junru Li, Jie Wei, Wenzhi Yang, Xiaohan Cai, Siqi Tian, Congshuo Chen, Yuzheng Wang, Peipei Zhao, Shan Cao, Wenhao Zhang, Jinxinyi Xu, Bin Gu, Fengqin Li, Peifeng Liu, Jun Pu

{"title":"DNA Nanoflower-Powered CRISPR/Cas12a Biosensing Platform for Ultrasensitive Protein Detection in Clinical Samples.","authors":"Chen Ji, Yongming Han, Junru Li, Jie Wei, Wenzhi Yang, Xiaohan Cai, Siqi Tian, Congshuo Chen, Yuzheng Wang, Peipei Zhao, Shan Cao, Wenhao Zhang, Jinxinyi Xu, Bin Gu, Fengqin Li, Peifeng Liu, Jun Pu","doi":"10.1002/smtd.202402130","DOIUrl":"https://doi.org/10.1002/smtd.202402130","url":null,"abstract":"Protein markers secreted by various human cells provide crucial insights for the early diagnosis and prognostic assessment of clinical diseases. However, restricted by efficient protein marker signal amplification in real clinical samples with complex compositions, accurate, sensitive, and rapid detection of protein markers remains largely challenging. Herein, a DNA nanoflower (DNF)-powered CRISPR/Cas12a biosensing platform (DNF-CRISPR) is presented that employs the DNF in upstream to amplify input signals for protein markers, while utilizing the CRISPR system in downstream to amplify output signals by trans cleavage. This upstream and downstream cascade amplification sensing platform exhibits high sensitivity (500 fg mL-1), rapid (≤2 h), and a broad dynamic range (2.5 pg mL-1 to 25 ng mL-1). As a proof of concept, DNF-CRISPR biosensing platform enables the quantitative detection of neutrophil gelatinase-associated lipocalin (NGAL) biomarkers in blood and urine samples from kidney injury patients with 91% accuracy. This study provides a powerful and versatile approach for the accurate diagnosis of protein markers in clinical settings, facilitating the application of CRISPR/Cas12a-based sensing platforms for non-nucleic acid markers.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2402130"},"PeriodicalIF":10.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Controlling the Local Conformation of RNA G-Quadruplex Results in Reduced RNA/Peptide Cytotoxic Accumulation Associated with C9orf72 ALS/FTD.

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-03-27 DOI: 10.1002/smtd.202401630

Saki Matsumoto, Hisae Tateishi-Karimata, Tatsuya Ohyama, Naoki Sugimoto

{"title":"Controlling the Local Conformation of RNA G-Quadruplex Results in Reduced RNA/Peptide Cytotoxic Accumulation Associated with C9orf72 ALS/FTD.","authors":"Saki Matsumoto, Hisae Tateishi-Karimata, Tatsuya Ohyama, Naoki Sugimoto","doi":"10.1002/smtd.202401630","DOIUrl":"https://doi.org/10.1002/smtd.202401630","url":null,"abstract":"Repeat expansion of d(G4C2) in the noncoding region of the C9orf72 gene contributes to neurodegenerative diseases. The repeat expansion transcript r(G4C2) induces RNA/peptide accumulation, which, in turn, induces cytotoxicity and accelerates the development of neurodegenerative diseases. Such cytotoxic accumulation is triggered by peptide aggregation. Here, a technique is developed to prevent accumulation by regulating RNA interactions, assuming that RNA structure is important for peptide interactions. A screening method is used to identify compounds that suppress RNA accumulation of r(G4C2) repeats. The four compounds are identified with wide π-planes containing hydroxyl, methoxy, and cyclic ether groups that suppressed RNA accumulation. Interestingly, these compounds also suppressed RNA/peptide accumulation in neuroblastoma cells, indicating that RNA accumulation is a key regulator of RNA/peptide cytotoxic aggregate formation. In vitro and in silico physicochemical analyses reveal that these compounds bind to the loop region of the G-quadruplex via hydrogen bonds or CH-π interactions, resulting in an altered loop conformation. Importantly, these conformational changes inhibited RNA G-quadruplex associations. These results show that conformational changes are promising for controlling the interactions between G-quadruplexes and further RNA accumulation. These findings may be useful in the development of therapeutic strategies for the treatment of neurodegenerative diseases.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2401630"},"PeriodicalIF":10.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anisotropic Superconductivity in Bilayer Kagome Borophene.

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-03-26 DOI: 10.1002/smtd.202402203

Haoxuan Zhang, Qian Gao, Xingxing Li, Yi Du, Zhenpeng Hu, Lan Chen

{"title":"Anisotropic Superconductivity in Bilayer Kagome Borophene.","authors":"Haoxuan Zhang, Qian Gao, Xingxing Li, Yi Du, Zhenpeng Hu, Lan Chen","doi":"10.1002/smtd.202402203","DOIUrl":"https://doi.org/10.1002/smtd.202402203","url":null,"abstract":"The unique electron configuration of boron allows it to form a rich variety of allotropes and exhibits great potential for superconductors. In this work, the superconductivity in bilayer Kagome borophene (BK-borophene) is investigated by first-principles calculations. The results show that BK-borophene is an anisotropic superconductor with strong electron-phonon coupling (EPC) and a high critical temperature (Tc) ≈17.4-35.0 K predicted by the anisotropic Migdal-Eliashberg equations and McMillan-Allen-Dynes formula. The superconductivity in BK-borophene is attributed to the strong EPC between electrons near the Fermi level and three phonon modes including the A2u mode and two Eg modes. The anisotropic superconductivity is due to the anisotropic EPC between the band related to the Dirac-like cone and the flat band. The electronic structure of BK-borophene has the van Hove singularity (VHS) and higher-order van Hove singularity (HOVHS) near the Fermi level, which can lead to the density of states divergence, charge accumulation, and EPC enhanced. The anisotropic EPC indicates that the HOVHS on the Dirac-like cone has the larger EPC strength, which may enhance the Tc. All these results indicate that BK-borophene holds great potential for applications in superconductivity. This work may benefit the research on boron-based materials and novel superconductors.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2402203"},"PeriodicalIF":10.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dislocation Introduction via Domain Engineering in Mg₂Sn Single Crystal to Improve its Thermoelectric Properties.

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-03-26 DOI: 10.1002/smtd.202500385

Zhicheng Huang, Kei Hayashi, Wataru Saito, Hezhang Li, Jun Pei, Jinfeng Dong, Toshiaki Chiba, Xue Nan, Bo-Ping Zhang, Jing-Feng Li, Yuzuru Miyazaki

{"title":"Dislocation Introduction via Domain Engineering in Mg2Sn Single Crystal to Improve its Thermoelectric Properties.","authors":"Zhicheng Huang, Kei Hayashi, Wataru Saito, Hezhang Li, Jun Pei, Jinfeng Dong, Toshiaki Chiba, Xue Nan, Bo-Ping Zhang, Jing-Feng Li, Yuzuru Miyazaki","doi":"10.1002/smtd.202500385","DOIUrl":"https://doi.org/10.1002/smtd.202500385","url":null,"abstract":"Dislocations have increasingly become important for improving the thermoelectric properties of thermoelectric materials due to their more pronounced scattering effect on phonons than on carriers. This study combined the introduction of the dislocation cores through domain engineering with the generation of Mg vacancies (VMg) by controlling point defects to achieve low lattice thermal conductivity and high power factor in n-type and p-type Mg2Sn single crystals (SCs). The VMg domain with ordered atomic arrangements allowed carrier transport with minimal scattering, while the high dislocation density at the interface effectively scattered phonons, thereby decoupling carrier-phonon transport. This resulted in obtaining the peak zT values of 0.83(8) and 0.42(4) for n-type and p-type Mg2Sn SCs, respectively. The outstanding combination of domain engineering and point defect control techniques could be a strategy for developing high-performance thermoelectric materials.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500385"},"PeriodicalIF":10.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA-Encoded Fluorescence Signals for Imaging Analysis.

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-03-26 DOI: 10.1002/smtd.202401303

Xiaowen Cao, Wenhao Fu, Xinyin Li, Feng Chen, Yongxi Zhao

{"title":"DNA-Encoded Fluorescence Signals for Imaging Analysis.","authors":"Xiaowen Cao, Wenhao Fu, Xinyin Li, Feng Chen, Yongxi Zhao","doi":"10.1002/smtd.202401303","DOIUrl":"https://doi.org/10.1002/smtd.202401303","url":null,"abstract":"Fluorescence imaging has been a powerful technique for the visualization of interest biomolecules. It is widely used in basic biological research, clinical medicine, and other fields. However, the fluorescence signals are often too weak for detecting with simple devices or in complex environments. Besides, fluorescence signals are limited to about four to six dyes, restricting to the spectral overlap of organic fluorophores. DNA nanotechnology including structured and dynamic DNA nanotechnology emerges as a promising material to encode fluorophores, holding great potential to improve the properties of fluorescence signals. Substantial progresses have been achieved in the DNA-encoded fluorescence signals, exhibiting novel characteristics and applications. This review summarizes various DNA encoding strategies with fluorescence signals and their performance in imaging analysis. In this review, different DNA encoding methods and their impacts on the fluorescence signals in imaging is reported, such as brightness, photostability, kinetics, and multiplexing. Besides, the biological application of DNA-encoded signals are reviewed. Finally, potential solutions to address current challenges in DNA-encoded fluorescence signals are suggested, encouraging the future development of this area.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2401303"},"PeriodicalIF":10.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gradient Doping Strategy for Sn─Pb Mixed Perovskite Solar Cells with High Efficiency and Stability.

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-03-26 DOI: 10.1002/smtd.202500064

Haotian Zhang, Chao Gao, Li He, Dezhao Zhang, Hongzhen Su, Hong Liu, Kadi Zhu, Wenzhong Shen

{"title":"Gradient Doping Strategy for Sn─Pb Mixed Perovskite Solar Cells with High Efficiency and Stability.","authors":"Haotian Zhang, Chao Gao, Li He, Dezhao Zhang, Hongzhen Su, Hong Liu, Kadi Zhu, Wenzhong Shen","doi":"10.1002/smtd.202500064","DOIUrl":"https://doi.org/10.1002/smtd.202500064","url":null,"abstract":"Sn─Pb hybrid perovskite has attracted more attention due to its ideal bandgap and excellent photoelectric properties. However, easy oxidation and poor crystallinity caused by the introduction of Sn2+ have become two major problems. In this study, Sn2+ is doped in the Pb-based perovskite to prepare high crystalline Sn─Pb mixed perovskite with larger grain size by using the solvent engineering technique and the cooperation optimization with HCOOH. The reducibility of HCOOH and its inhibition of deprotonation significantly prevent the oxidation of Sn2+ and the decomposition of A-site cations. The experimental and theoretical results show that the interactions between HCOOH and Sn2+ and Pb2+, which reduce the defect density and improve the crystallinity and stability of the film with excellent photoelectric properties. In addition, the compact SnO2 prepared by atomic layer deposition as electronic transformation layer to further improve the stability of devices. The photoelectric conversion efficiency of the Sn─Pb hybrid perovskite solar cells (PSCs) prepared by the dopant growth method can reach 21.53% and the stability is significantly better than that of the Sn─Pb PSCs prepared by the traditional method.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500064"},"PeriodicalIF":10.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Refined Protocol for Improving Accuracy and Reliability in Urea Quantification During Electrochemical C─N Coupling Reactions.

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-03-24 DOI: 10.1002/smtd.202401336

Weidong Dai, Shiyong Mou, Siyuan Liu, Peng Xu, Chiyao Tang, Keying Wu, Xing'an Dong, Lei Xiao, Chang Long, Fan Dong

{"title":"Refined Protocol for Improving Accuracy and Reliability in Urea Quantification During Electrochemical C─N Coupling Reactions.","authors":"Weidong Dai, Shiyong Mou, Siyuan Liu, Peng Xu, Chiyao Tang, Keying Wu, Xing'an Dong, Lei Xiao, Chang Long, Fan Dong","doi":"10.1002/smtd.202401336","DOIUrl":"https://doi.org/10.1002/smtd.202401336","url":null,"abstract":"Promoted by the growing demand for sustainable carbon and nitrogen cycling, electrochemical C─N coupling in urea synthesis has attracted intensive interest. Urea quantification provides the basis for an in-depth understanding of structure-performance correlations and the iterative optimization of performance. However, current urea quantification methods have non-negligible drawbacks, largely bringing about false positive or negatives risks. Herein, the most accessible and commonly used quantification techniques, namely the diacetylmonoxime-thiosemicarbazide and Urease-based methods, are comprehensively re-assessed. The adverse impacts caused by nitrite, metallic impurities, and standard curve protocol are thoroughly identified. Most importantly, direct problem-solving strategies are proposed to rule out those confounding factors by chemical pre-reduction, galvanic replacement, and refinement of the standard curve protocol, respectively. This refined urea quantification method can solidify the reproduction of urea electrosynthesis studies and thus promote the sound development of this emerging field.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2401336"},"PeriodicalIF":10.7,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0