Small Methods最新文献

DNA Nanostructures-Based In Situ Cancer Vaccines: Mechanisms and Applications.

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-01-22 DOI: 10.1002/smtd.202401501

Bingyu Lin, Yanfei Liu, Qiwen Chen, Mingfeng Li, Lishang Xu, Qianqian Chen, Yifu Tan, Zhenbao Liu

{"title":"DNA Nanostructures-Based In Situ Cancer Vaccines: Mechanisms and Applications.","authors":"Bingyu Lin, Yanfei Liu, Qiwen Chen, Mingfeng Li, Lishang Xu, Qianqian Chen, Yifu Tan, Zhenbao Liu","doi":"10.1002/smtd.202401501","DOIUrl":"https://doi.org/10.1002/smtd.202401501","url":null,"abstract":"Current tumor vaccines suffer from inadequate immune responsive due to the insufficient release of tumor antigens, low tumor infiltration, and immunosuppressive microenvironment. DNA nanostructures with their ability to precisely engineer, controlled release, biocompatibility, and the capability to augment the immunogenicity of tumor microenvironment, have gained significant attention for their potential to revolutionize vaccine designing. This review summarizes various applications of DNA nanostructures in the construction of in situ cancer vaccines, which can generate tumor-associated antigens directly from damaged tumors for cancer immune-stimulation. The mechanisms and components of cancer vaccines are listed, the specific strategies for constructing in situ vaccines using DNA nanostructures are explored and their underlying mechanisms of action are elucidated. The immunogenic cell death (ICD) induced by chemotherapeutic agents, photothermal therapy (PTT), photodynamic therapy (PDT), and radiation therapy (RT) and the related cancer vaccines building strategies are systematically summarized. The applications of different DNA nanostructures in various cancer immunotherapy are elaborated, which exerts precise, long-lasting, and robust immune responses. The current challenges and future prospectives are proposed. This review provides a holistic understanding of the evolving role of DNA nanostructures for in situ vaccine development.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2401501"},"PeriodicalIF":10.7,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circular Single-Stranded DNA-Based Artificial Nanoviruses Mitigate Colorectal Cancer Development.

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-01-21 DOI: 10.1002/smtd.202402069

Jinghao Wang, Pengfei Zhang, Yonglian Huang, Guang Hu, Kexuan Zou, Songtao Zhou, Dandan Shao, Jianming Wang, Jie Song

{"title":"Circular Single-Stranded DNA-Based Artificial Nanoviruses Mitigate Colorectal Cancer Development.","authors":"Jinghao Wang, Pengfei Zhang, Yonglian Huang, Guang Hu, Kexuan Zou, Songtao Zhou, Dandan Shao, Jianming Wang, Jie Song","doi":"10.1002/smtd.202402069","DOIUrl":"https://doi.org/10.1002/smtd.202402069","url":null,"abstract":"Colorectal cancer (CRC) remains a significant global health challenge, underscoring the need for innovative therapeutic strategies. Oncogenic miRNAs (oncomiRs) play a significant biological role in the initiation and progression of colorectal cancer. Inspired by the cooperative mechanisms of plant nanovirus, which employ multiple circular single-stranded DNA (CssDNA) genomes, it is hypothesized that the development and delivery of CssDNA to target oncomiRs would achieve therapeutic benefits in CRC. In this study, a multi-omics approach is utilized to identify key tumor suppressor genes (TSGs) and their related oncomiRs implicated in CRC, followed by the development of CssDNA, each of which is loaded with multiple miRNA binding sites targeting one oncomiR. When transfected into the cells, these CssDNA can effectively target and sequester the corresponding oncomiRs to restore the expression of TSGs, leading to a marked reduction in CRC development both in vitro and in vivo. The findings highlight the therapeutic potential of nanovirus-inspired CssDNA in modulating the miRNA-mediated regulatory network in CRC. This study lays the groundwork for the development of non-coding DNA-based therapies with broad implications for the treatment of colorectal cancer and potentially other malignancies.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2402069"},"PeriodicalIF":10.7,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Graphene-Based Opto-Electronic Platform for Ultra-Sensitive Biomarker Detection at Zeptomolar Concentrations.

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-01-21 DOI: 10.1002/smtd.202402026

Matteo Piscitelli, Cinzia Di Franco, Giuseppe Valerio Bianco, Giovanni Bruno, Eleonora Macchia, Luisa Torsi, Gaetano Scamarcio

{"title":"Graphene-Based Opto-Electronic Platform for Ultra-Sensitive Biomarker Detection at Zeptomolar Concentrations.","authors":"Matteo Piscitelli, Cinzia Di Franco, Giuseppe Valerio Bianco, Giovanni Bruno, Eleonora Macchia, Luisa Torsi, Gaetano Scamarcio","doi":"10.1002/smtd.202402026","DOIUrl":"https://doi.org/10.1002/smtd.202402026","url":null,"abstract":"A ground-breaking graphene-based biosensor designed for label-free detection of immunoglobulin M (IgM) achieving a remarkable concentration of 100 zeptomolar (10-19 m), is reported. The sensor is a two-terminal device and incorporates a millimeter-wide gold interface, bio-functionalized with ≈1012 anti-IgM antibodies and capacitively coupled to a bare graphene electrode through a water-soaked paper strip. In this configuration, few affinity binding events trigger a collective electrostatic reorganization of the protein layer, leading to an extended surface potential (SP) shift of the biofunctionalized Au surface. The SP shift, mediated by electrolyte capacitive coupling, induces a corresponding shift in the Fermi level of graphene. This shifts the graphene phonon frequencies, which are measured by Raman spectroscopy. Decoupling the sensing interface from the transducing graphene layer provides flexibility in surface chemistry modifications, while preserving the graphene integrity. A key aspect of this biosensor is its ability to precisely determine the graphene charge neutrality point from the voltage dependence of phonon frequency shifts, enabling detections of biomarker at unprecedented low concentrations. The integration of graphene with optical probing demonstrates a proof-of-concept and establishes a ground-breaking approach to in situ biomarker detection, setting the stage for a future generation of portable opto-electronic high-performance diagnostic tools for single-marker detection.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2402026"},"PeriodicalIF":10.7,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-Mode ZnO/SnSe Heterojunction Devices with Integrated Bipolar Response Photodetectors and Artificial Optoelectronic Synapses for in-Sensor Computing.

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-01-21 DOI: 10.1002/smtd.202402151

Fuhai Guo, Weizhuo Yu, Mingcong Zhang, Bo Zhang, Bing Hu, Shuangshuang Li, Jianyu Jiang, Ankai Sun, Yunjie Liu, Lanzhong Hao

{"title":"Dual-Mode ZnO/SnSe Heterojunction Devices with Integrated Bipolar Response Photodetectors and Artificial Optoelectronic Synapses for in-Sensor Computing.","authors":"Fuhai Guo, Weizhuo Yu, Mingcong Zhang, Bo Zhang, Bing Hu, Shuangshuang Li, Jianyu Jiang, Ankai Sun, Yunjie Liu, Lanzhong Hao","doi":"10.1002/smtd.202402151","DOIUrl":"https://doi.org/10.1002/smtd.202402151","url":null,"abstract":"Optoelectronic synapse devices (OESDs) inspired by human visual systems enable to integration of light sensing, memory, and computing functions, greatly promoting the development of in-sensor computing techniques. Herein, dual-mode integration of bipolar response photodetectors (PDs) and artificial optoelectronic synapses based on ZnO/SnSe heterojunctions are presented. The function of the fabricated device can be converted between the PDs and OESDs by modulating the light intensity. As a PD, the polarity of the output current can be switched by tuning the laser wavelength and intensity, which is attributed to the competition between the photovoltaic and photothermoelectric effects in the ZnO/SnSe heterojunction. As an OESD, the device exhibits versatile photonic synaptic characteristics at low light intensity based on the defect-dominant carrier trapping/de-trapping processes, including short/long-term plasticity and learning experience behaviors. More importantly, benefitting from the outstanding synaptic responses, logic functions including \"AND\" and \"OR\" are implemented through the in-sensor computing architecture. This work supplies a novel route to realize complex functionality in one device and offers effective strategies for developing in-sensor computing.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2402151"},"PeriodicalIF":10.7,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacteria Flagella-Mimicking Polymer Multilayer Magnetic Microrobots.

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-01-21 DOI: 10.1002/smtd.202401558

Liang Lu, Shuang Bai, Jiaqi Shi, Hutao Zhang, Gang Hou, Wei Wang, Shoubin Sun, Tianyun Huang, Yuxin Jia, Alexander Granovsky, Perov Nikolai, Zhiguang Wu, Hui Xie, He Wu

{"title":"Bacteria Flagella-Mimicking Polymer Multilayer Magnetic Microrobots.","authors":"Liang Lu, Shuang Bai, Jiaqi Shi, Hutao Zhang, Gang Hou, Wei Wang, Shoubin Sun, Tianyun Huang, Yuxin Jia, Alexander Granovsky, Perov Nikolai, Zhiguang Wu, Hui Xie, He Wu","doi":"10.1002/smtd.202401558","DOIUrl":"https://doi.org/10.1002/smtd.202401558","url":null,"abstract":"Mass production of biomedical microrobots demands expensive and complex preparation techniques and versatile biocompatible materials. Learning from natural bacteria flagella, the study demonstrates a magnetic polymer multilayer cylindrical microrobot that bestows the controllable propulsion upon an external rotating magnetic field with uniform intensity. The magnetic microrobots are constructed by template-assisted layer-by-layer technique and subsequent functionalization of magnetic particles onto the large opening of the microrobots. Geometric variables of the polymer microrobots, such as the diameter and wall thickness, can be controlled by selection of porous template and layers of assembly. The microrobots perform controllable propulsion through the manipulation of magnetic field. The comparative analysis of the movement behavior reveals that the deformation of microrobots may be attributed to the propulsion upon rotating magnetic field, which is similar to that of natural bacteria. The influence of actuation and frequency on the velocity of the microrobots is studied. Such polymer multilayer magnetic microrobots may provide a novel concept to develop rapidly delivering drug therapeutic agents for diverse practical biomedical uses.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2401558"},"PeriodicalIF":10.7,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Employing a Zn-air/Photo-Electrochemical Cell for In Situ Generation of H₂O₂ for Onsite Control of Pollutants.

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-01-19 DOI: 10.1002/smtd.202401539

Asutosh Behera, Aninda Jiban Bhattacharyya

{"title":"Employing a Zn-air/Photo-Electrochemical Cell for In Situ Generation of H2O2 for Onsite Control of Pollutants.","authors":"Asutosh Behera, Aninda Jiban Bhattacharyya","doi":"10.1002/smtd.202401539","DOIUrl":"https://doi.org/10.1002/smtd.202401539","url":null,"abstract":"Industrial production of hydrogen peroxide (H2O2) is energy-intensive and generates unwanted byproducts. Herein, an alternative production strategies of H2O2 are demonstrated in a Zn-air and a photoelectrochemical cell. Employing an optimally produced reduced graphene oxide (rGO) electrocatalyst@air-cathode, an impressive power density of 320 Wmgeo -2 (geo = geometric area) is achieved along with a high H2O2 production rate of 3.17 mol mgeo -2h-1 (operating potential = 0.8 V). Systematic investigations reveal the critical role of specific functional groups (viz. C─O─C, chemisorbed O2, C≐C) to be responsible for enhancing the yield of H2O2. The in situ generated superoxide (O2˙) and hydroxyl radicals (˙OH) act as oxidants to efficiently degrade onsite, a model textile dye pollutant (viz. rhodamine B) inside the Zn-air cell. Using the identical rGO as the photoelectrode in an H-type cell, the H2O2 production is remarkably enhanced under visible light illumination. Simultaneously, the onsite pollutant degradation occurs five times faster than the Zn-air cell (at the same operating potential = 0.8 V). This work opens a new paradigm for electrosynthesis, wherein an underlying redox can be utilized to synthesize industrial chemicals for onsite control of environmental pollution sustainably.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2401539"},"PeriodicalIF":10.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autonomous Self-Healing Magnetoelectric I-Skin from Self-Bonded Deep Eutectic Polymer.

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-01-19 DOI: 10.1002/smtd.202402190

Yixuan Wu, Ling Cai, Zhuofan Li, Guangxue Chen, Sushila Maharjan, Minghui He, Bin Su

{"title":"Autonomous Self-Healing Magnetoelectric I-Skin from Self-Bonded Deep Eutectic Polymer.","authors":"Yixuan Wu, Ling Cai, Zhuofan Li, Guangxue Chen, Sushila Maharjan, Minghui He, Bin Su","doi":"10.1002/smtd.202402190","DOIUrl":"https://doi.org/10.1002/smtd.202402190","url":null,"abstract":"Next-generation ionic skin (i-skin) should be self-healing and self-powered, promoting its development toward lightweight, miniaturization, compact, and portable designs. Previously reported self-powered i-skin mostly either lack the ability to self-repair damaged parts or only have self-healing capabilities some components, falling short of achieving complete device self-healability. In this work, a self-bonding strategy is presented to obtain an all-polymerizable deep eutectic solvent (PDES) magnetoelectric i-skin (MIS) that simultaneously achieves self-powering and full-device autonomous self-healability. The three-layered MIS can easily restore mechanical and electrochemical performance at the full-device level without requiring any external stimulus. The developed MIS can be easily configured into various 3D architectures with highly compatible magnetic and conductive components, offering promising potential for the advancement of embodied energy technologies. The present work provides a versatile and user-friendly platform for producing a wide range of intrinsic self-healing multi-layered devices made from soft materials, with potential applications extending beyond human-machine interfaces and artificial intelligence.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2402190"},"PeriodicalIF":10.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromium-Doped NiBP Micro-Sphere Electrocatalysts for Green Hydrogen Production under Industrial Operational Conditions.

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-01-19 DOI: 10.1002/smtd.202401939

Md Ahasan Habib, Shusen Lin, Sumiya Akter Dristy, Mehedi Hasan Joni, Rutuja Mandavkar, Jae-Hun Jeong, Jihoon Lee

{"title":"Chromium-Doped NiBP Micro-Sphere Electrocatalysts for Green Hydrogen Production under Industrial Operational Conditions.","authors":"Md Ahasan Habib, Shusen Lin, Sumiya Akter Dristy, Mehedi Hasan Joni, Rutuja Mandavkar, Jae-Hun Jeong, Jihoon Lee","doi":"10.1002/smtd.202401939","DOIUrl":"https://doi.org/10.1002/smtd.202401939","url":null,"abstract":"Wide spread adaptation of green hydrogen can help to mitigate the serious climate issues, increasing global energy demands and the development of advanced electrocatalysts robust under industrial conditions is one of the key technological challenges. Herein, chromium-doped nickel-boride-phosphide (Cr/NiBP) micro sphere (MS) electrocatalyst is demonstrated via a two-step hydrothermal approach along with post-annealing. The Cr/NiBP MS demonstrates low hydrogen evolution reaction and oxygen evaluation reaction over potentials of 78 and 250 mV at 100 mA cm-2 in 1 m KOH, out performing most of the reported catalysts. The Cr/NiBP ǁ Cr/NiBP exhibits only 1.54 V at 100 mA cm-2 in 1 m KOH and surpasses the benchmark of RuO2 (+) ǁ Pt/C (-) up to 2000 mA cm-2, which sets it as one of the best bifunctional electrocatalysts. Impressively, it maintains stable performance for over 240 h at 1000 mA cm-2 in 6 m KOH at 60°C, demonstrating rapid response, anti-corrosion resistance, and robust structural integrity to meet the industrial operational conditions. Further, Cr/NiBP ǁ Pt/C exhibits a super-low cell-voltage of 2.25 V at 2000 mA cm-2. The small amount of Cr atoms incorporation can significantly enhance active sites and intrinsic properties, accelerating water dissociation and rapid intermediate formation.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2401939"},"PeriodicalIF":10.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Patterning of Photochromic Diarylethene Crystals by Sublimation for Morphological Controls.

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-01-19 DOI: 10.1002/smtd.202401545

Mami Isobe, Daichi Kitagawa, Seiya Kobatake

引用次数: 0

Graphene Oxide Foam-Based Floating Actuators Manipulated via Dual-Marangoni-Effect Propulsion and Magnetic-Field-Guided Navigation.

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-01-19 DOI: 10.1002/smtd.202401946

Wei Wang, Wei Shao, Ning Li, Heng-Yu Guo, Su-Chuan Zeng, Yang Zhang, Jia-Rui Zhang, Dong-Dong Han, Yong-Lai Zhang

{"title":"Graphene Oxide Foam-Based Floating Actuators Manipulated via Dual-Marangoni-Effect Propulsion and Magnetic-Field-Guided Navigation.","authors":"Wei Wang, Wei Shao, Ning Li, Heng-Yu Guo, Su-Chuan Zeng, Yang Zhang, Jia-Rui Zhang, Dong-Dong Han, Yong-Lai Zhang","doi":"10.1002/smtd.202401946","DOIUrl":"https://doi.org/10.1002/smtd.202401946","url":null,"abstract":"Intelligent stimuli-responsive actuators that can convert environmental energies into mechanical works have garnered significant research interests. Among different actuation principles, Marangoni effect is distinguished due to simplicity, high efficiency, remote manipulation, and water environment adaptability. Nevertheless, both chemical and physical Marangoni actuators face their own challenges with respect to limited chemical loading, precise light illumination, and relatively poor motion controllability. In this study, floating actuators based on graphene oxide foam (GOF), manipulable via dual Marangoni effects and magnetic field, are fabricated by Direct Laser Writing (DLW). This is the first work to realize dual-Marangoni-effect actuators. Specifically, it is observed that the actuator driven by the chemical Marangoni effect can attain an average speed of 0.57 rad s-1. Meanwhile, the actuator driven by the photothermal Marangoni effect is capable of reaching an average speed of 0.17 rad s-1, and the average speed is 1.34 cm s-1 under the manipulation of magnetic field. Multi-field coupling and dual Marangoni effects make actuators more flexible and intelligent, with promising potential for intelligent control and biomedical engineering.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2401946"},"PeriodicalIF":10.7,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0