Nano TodayPub Date : 2025-02-08DOI: 10.1016/j.nantod.2025.102649
Pritam K. Chakraborty , Stephanie E. Wolf , Govind Ummethala , Ansgar Meise , Tobias Mehlkoph , Junbeom Park , Marc Heggen , Amir H. Tavabi , Vaibhav Vibhu , André Karl , Eva Jodat , L.G.J. (Bert) de Haart , Rafal E. Dunin-Borowski , Shibabrata Basak , Rüdiger-A. Eichel
{"title":"Unveiling the exsolution mechanisms and investigation of the catalytic processes of Sr2FeMo0.65Ni0.35O6-δ using in situ transmission electron microscopy","authors":"Pritam K. Chakraborty , Stephanie E. Wolf , Govind Ummethala , Ansgar Meise , Tobias Mehlkoph , Junbeom Park , Marc Heggen , Amir H. Tavabi , Vaibhav Vibhu , André Karl , Eva Jodat , L.G.J. (Bert) de Haart , Rafal E. Dunin-Borowski , Shibabrata Basak , Rüdiger-A. Eichel","doi":"10.1016/j.nantod.2025.102649","DOIUrl":"10.1016/j.nantod.2025.102649","url":null,"abstract":"<div><div>Solid oxide cells (SOCs) are likely to play crucial role in the green energy transition, but their widespread adoption is hindered by degradation issues, particularly catalyst agglomeration. Nanoparticle exsolution in double-perovskite materials offers a promising solution by creating electrode materials with stable metallic nanocatalysts strongly bonded to the parent oxide, mitigating high-temperature agglomeration issues. Thus, understanding the dynamic evolution of microstructure and catalytic behavior in such materials is vital for developing high-performing SOC catalysts. This study utilized a multimodal approach to investigate the dynamics of exsolution in Sr<sub>2</sub>FeMo<sub>0.65</sub>Ni<sub>0.35</sub>O<sub>6-δ</sub> (SFM-Ni) and its effect on cell performance. <em>In situ</em> environmental transmission electron microscopy (ETEM), <em>in situ</em> transmission electron microscopy (TEM) coupled with mass spectrometry visualized the formation and the stability of exsolved particles especially at the concave faces of the parent material during chemical conversion of CO from CO<sub>2</sub>. Simultaneously, macro-scale cell experiments coupled with electrochemical impedance spectroscopy, and focused ion beam-scanning electron microscopy (FIB-SEM) tomography, apart from verifying the nanoscale observations, provided crucial insights into the correlation between the exsolution process observed at the micro-scale and the overall cell performance. These findings offers valuable insights into the design and optimization of improved electrode materials for SOCs. Understanding the dynamic behavior of exsolved catalysts would help in enhancing the electrochemical performance at both the nano and macro levels, ultimately advancing the field of sustainable energy technologies.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"61 ","pages":"Article 102649"},"PeriodicalIF":13.2,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143351159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nano TodayPub Date : 2025-02-07DOI: 10.1016/j.nantod.2025.102659
Xinyue Cao , Minhui Lu , Jinglin Wang , Yu Wang , Yuanjin Zhao
{"title":"Verteporfin-integrated conductive zwitterionic hydrogels for scarless wound management","authors":"Xinyue Cao , Minhui Lu , Jinglin Wang , Yu Wang , Yuanjin Zhao","doi":"10.1016/j.nantod.2025.102659","DOIUrl":"10.1016/j.nantod.2025.102659","url":null,"abstract":"<div><div>Hydrogels have outstanding values in wound management. Endeavors in this area have been concentrated on the improvement of the inherent functions of hydrogels and the cooperation with effective bioactives, achieving scarless wound therapy. Herein, we develop a novel verteporfin (VP)-integrated conductive zwitterionic [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl) (SBMA) hydrogels for promoting wound healing process and prevention scar formation. The hydrogels with VP’s encapsulation are formed through PEDOT:PSS-promoted spontaneous polymerization of SBMA. The obtained dual-conductive hydrogels possess high resilience, desired adhesion, and inherent antibacterial property, making them extremely suitable for treating skin defects through close contact with wound area, infection reduction and lesions’ electrical field enhancement. Additionally, the loaded VP can be constantly released to wound sites and exhibits anti-scarring effect by inhibiting the Yes-Associated Protein activation and collagen deposition. Based on these advantages, our hydrogel patch has been proven to achieve satisfying therapeutic effects for <em>in vivo</em> scarless wound management. Thus, we believe that the proposed VP-integrated conductive SBMA hydrogels are valuable as multifunctional dressings for wound and scar management.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"61 ","pages":"Article 102659"},"PeriodicalIF":13.2,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143306452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nano TodayPub Date : 2025-02-06DOI: 10.1016/j.nantod.2025.102657
Taoxing Peng , Yihao He , Xinyue Shao , Han Liu , Lu Yang , Wei Xiong , Xiaoying Jin , Yang Ding , Yongzhuo Huang
{"title":"Reversing tumor fibrosis for potentiating chemo-immunotherapy via lactic acid exhaustion by codelivery of bicarbonate and JQ1","authors":"Taoxing Peng , Yihao He , Xinyue Shao , Han Liu , Lu Yang , Wei Xiong , Xiaoying Jin , Yang Ding , Yongzhuo Huang","doi":"10.1016/j.nantod.2025.102657","DOIUrl":"10.1016/j.nantod.2025.102657","url":null,"abstract":"<div><div>Tumor fibrosis imposes a formidable barrier against effective chemo-immunotherapy, typically resulting in limited infiltration of immune cells and poor drug penetration into the tumor. In this study, it was revealed that intratumoral lactic acid exhaustion can relieve tumor fibrosis by suppressing TGF-β pathway. The bicarbonate liposomes loaded with JQ1 (JQ1/NaHCO<sub>3</sub>@TLip) was constructed, with surface modification with lactoferrin (LF) that enabled tumor-targeting delivery via binding with LRP-1 receptors. Intratumoral lactic acid can be reduced via both neutralization and epigenetic-metabolism regulation. JQ1/NaHCO<sub>3</sub>@TLip was demonstrated to effectively reverse tumor fibrosis, thereby promoting immune cell infiltration, and enhancing deep penetration of both nanomedicine (e.g., liposomal doxorubicin (DOX@Lip)) and protein drugs (e.g., anti-PD-1). Importantly, the combination of low-dose DOX@Lip and JQ1/NaHCO<sub>3</sub>@TLip exhibited remarkable efficacy with reducing side toxicity. This combination approach also effectively inhibited tumor recurrence and distant metastasis. Moreover, pretreatment with JQ1/NaHCO<sub>3</sub>@TLip showed a synergistic effect on enhancing tumor inhibition efficacy of anti-PD-1 therapy. These findings indicate that JQ1/NaHCO<sub>3</sub>@TLip is promising as a nano-adjuvant to enhance chemo-immunotherapy via lactic acid exhaustion.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"61 ","pages":"Article 102657"},"PeriodicalIF":13.2,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143306451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A dual-warhead high-density lipoprotein mimetic nanomedicine simultaneously regulates stromal extracellular matrix and cGAS/STING activation for potent immunotherapy of cancer","authors":"Weiwei Wu , Xiaoyang Gao , Haina Tian , Haiyun Wei , Zongyao Ding , Qian Ming , Xue Liang , Changlong Wang","doi":"10.1016/j.nantod.2025.102656","DOIUrl":"10.1016/j.nantod.2025.102656","url":null,"abstract":"<div><div>Immunotherapy has shown great potential for cancer treatment in the clinic. However, the inherent physical barrier of abundant extracellular matrix (ECM) and the biological barrier of immunosuppressive microenvironment have greatly hindered the intratumoral infiltration of effector T cells. Herein, a dual-warhead high-density lipoprotein (HDL) mimetic nanomedicine is constructed that can regulate stromal ECM and cyclic guanosine monophosphate adenosine monophosphate synthase (cGAS)-stimulator of interferon genes (STING) activation for potent immunotherapy of cancer. After coating the HDL-mimetic outer layer and encapsulation of the transforming growth factor beta (TGF-β) receptor inhibitor LY3200882 (LY) on the hollow manganese dioxide nanoparticles loaded with glucose oxidase, the nanomedicine can target the tumor tissue with high efficiency. In the weak acidic tumor microenvironment, LY is released from the nanomedicine and destroys the physical barrier of ECM by inhibition the TGF-β receptor. After being endocytosed by tumor cells, the manganese ion is released and promotes the self-enhanced chemodynamic therapy. The damage-associated molecular patterns release and the cGAS/STING signaling pathway activation together destroy the biological barrier of immunosuppressive microenvironment. This nanomedicine thus shows potent antitumor efficiency on subcutaneous tumor model, tumor metastasis mice model and tumor recurrence mice model, providing a new strategy for amplifying the cancer immunotherapy.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"61 ","pages":"Article 102656"},"PeriodicalIF":13.2,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143306450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nano TodayPub Date : 2025-02-03DOI: 10.1016/j.nantod.2025.102645
Luka Blagojevic, Nazila Kamaly
{"title":"Nanogels: A chemically versatile drug delivery platform","authors":"Luka Blagojevic, Nazila Kamaly","doi":"10.1016/j.nantod.2025.102645","DOIUrl":"10.1016/j.nantod.2025.102645","url":null,"abstract":"<div><div>Nanogels are crosslinked polymeric nanoparticles that exhibit characteristics of an ideal vehicle for therapeutics delivery. These nanocarriers are highly tunable due to the ability of using a variety of chemistries and building blocks in their design, facilitating tailoring of their overall size, surface charge, and payload release behavior. Furthermore, nanogels are compatible with a range of payloads such as small molecules and macromolecules. The level of control over drug loading and drug release parameters achievable <em>via</em> nanogels is superior to common nanocarriers due to this platform’s chemical flexibility, which is amenable to bottom-up and fully covalent synthetic approaches. The modular nature of the nanogel platform facilitates many variations in nanoparticle design, resulting in nanomaterials with complex morphologies, hybrid physicochemical properties and responsiveness to environmental stimuli. “Smart” nanogels are capable of triggered release of the encapsulated payload in response to pH modifications, presence of enzymes, temperature fluctuations, and changes in the concentration of reductants and oxidizers in the surrounding milieu, in addition to physical triggers such as heat and light. The bioconjugation of nanogels with targeting elements yields highly selective multifunctional entities with potential for precision drug delivery applications. This review aims at providing a comprehensive summary of chemical synthesis methods for nanogel development for drug delivery applications. Aspects regarding targeting, encapsulation mechanisms and the reactivity of stimuli-responsive nanogels are covered with the intent of giving the reader an insight in the fundamental chemical principles influencing the rational design of nanogels for drug delivery.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"61 ","pages":"Article 102645"},"PeriodicalIF":13.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143175584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nano TodayPub Date : 2025-02-03DOI: 10.1016/j.nantod.2025.102655
Yunji Lee , Katie McAllister , Hwa-Rim Lee , Sungjune Jung , Fiona Murphy
{"title":"Evaluation of a bioprinted 3D airway tissue model for toxicity testing of nanomaterials; Pathway to integration into a tiered testing strategy for hazard assessment to support safety-by-design","authors":"Yunji Lee , Katie McAllister , Hwa-Rim Lee , Sungjune Jung , Fiona Murphy","doi":"10.1016/j.nantod.2025.102655","DOIUrl":"10.1016/j.nantod.2025.102655","url":null,"abstract":"<div><div>There is an urgent need to identify hazards posed by novel nanomaterials (NMs), however, new models are required to streamline testing approaches, increase our understanding of mechanism of toxicity and incorporate Safer-by-Design concepts into NM development. Here, we conducted an evaluation of a sophisticated 3D bioprinted airway model for a first-in-kind hazard assessment of NMs. Exploiting the consistency and reproducibility provided by bioprinting techniques, airway constructs were generated by precisely controlled sequential layering of endothelial cells, fibroblasts embedded in collagen, and bronchial epithelial cells and exposed to ZnO and BaSO<sub>4</sub> NMs case-study materials. Exposure to ZnO resulted in greater temporal and dynamic immunotoxic and histological responses in contrast to BaSO<sub>4</sub>, demonstrating the successful differentiation of high and low reactivity NMs. Comparison with simple <em>in vitro</em> toxicity studies and existing <em>in vivo</em> and human data demonstrated the physiological relevance of increasing model complexity. The relative benefits of the 3D bioprinted airway model and potential for inclusion in tiered testing strategies was substantiated by comprehensive review of the current state-of-the-art alternative models. This study supports the wider adoption of a sophisticated <em>in vitro</em> airway model to reduce our reliance on <em>in vivo</em> testing and advance efforts to develop Integrated Approaches to Assessment and Testing to support Safer-by-Design NM innovation.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"61 ","pages":"Article 102655"},"PeriodicalIF":13.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143175636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nano TodayPub Date : 2025-02-01DOI: 10.1016/S1748-0132(24)00441-9
{"title":"Outside Back Cover - Graphical abstract TOC/TOC in double column/Cover image legend if applicable, Bar code, Abstracting and Indexing information","authors":"","doi":"10.1016/S1748-0132(24)00441-9","DOIUrl":"10.1016/S1748-0132(24)00441-9","url":null,"abstract":"","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"60 ","pages":"Article 102585"},"PeriodicalIF":13.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nano TodayPub Date : 2025-02-01DOI: 10.1016/j.nantod.2025.102650
Qinzhen Cheng , Gaoli Niu , Yiling He , Yalan Zhu , Yong Kang , Zhuhong Zhang , Xiaoyuan Ji
{"title":"Innovative design of bacterial outer membrane vesicles for vaccine development","authors":"Qinzhen Cheng , Gaoli Niu , Yiling He , Yalan Zhu , Yong Kang , Zhuhong Zhang , Xiaoyuan Ji","doi":"10.1016/j.nantod.2025.102650","DOIUrl":"10.1016/j.nantod.2025.102650","url":null,"abstract":"<div><div>Bacterial outer membrane vesicles (OMVs) have emerged as a highly promising platform for vaccine development. However, the inherent limitations of native OMVs, including restricted immunogenicity and a lack of specificity, have emphasized the necessity for comprehensive modification and engineering. This comprehensive review delves deeply into the biogenesis, structures, and compositions of OMVs, providing a thorough exploration of their potential. Moreover, this review elucidates engineering strategies—encompassing genetic, chemical, and physical engineering—that have been employed to optimize the immunogenicity and stability of OMVs. Additionally, the review sheds light on the evolving applications of OMVs in the realm of vaccines, emphasizing their remarkable potential as pioneering vaccine platforms. Essentially, this review offers a comprehensive understanding of engineered OMVs for vaccine applications, thereby fostering further research and innovation in this pivotal domain.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"61 ","pages":"Article 102650"},"PeriodicalIF":13.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143176724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nano TodayPub Date : 2025-01-31DOI: 10.1016/j.nantod.2025.102654
Wathsala Liyanage , Gokul R. Kannan , Sujatha Kannan , Rangaramanujam M. Kannan
{"title":"Efficient intracellular delivery of CRISPR-Cas9 ribonucleoproteins using dendrimer nanoparticles for robust genomic editing","authors":"Wathsala Liyanage , Gokul R. Kannan , Sujatha Kannan , Rangaramanujam M. Kannan","doi":"10.1016/j.nantod.2025.102654","DOIUrl":"10.1016/j.nantod.2025.102654","url":null,"abstract":"<div><div>CRISPR-Cas9, a flexible and efficient genome editing technology, is currently limited by the challenge of delivering the large ribonucleoprotein complex intracellularly and into the nucleus. Existing delivery techniques/vectors are limited by their toxicity, immunogenicity, scalability, and lack of specific cell-targeting ability. This study presents a neutral, non-toxic dendrimer conjugate construct that shows promise in overcoming these limitations. We covalently-conjugated S. pyogenes Cas9–2NLS (Cas9-nuclear localization sequence) endonuclease to a generation-6 hydroxyl PAMAM dendrimer through a glutathione-sensitive disulfide linker <em>via</em> highly specific inverse Diels-alder click reaction (IEDDA), and a single guide RNA (sgRNA) was complexed to the Cas9-dendrimer conjugate nano-construct (D-Cas9). D-Cas9- RNP produces robust genomic deletion <em>in vitro</em> of GFP in HEK293 cells (∼100 %) and VEGF in a human pigmental epithelium cell line (ARPE-19) (20 %). The uptake of the D-Cas9-RNP constructs on similar timescales as small molecules highlights the robustness of the biophysical mechanisms enabling the dendrimer to deliver payloads as large as Cas9, while retaining payload functionality. This promising conjugation approach enabled better stability to the neutral construct. Combined with recent advances in hydroxyl dendrimer delivery technologies in the clinic, this approach may lead to advances in ‘neutral’ dendrimer-enabled non-toxic, cell-specific, highly efficient <em>in vitro</em> and <em>in vivo</em> genome editing.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"61 ","pages":"Article 102654"},"PeriodicalIF":13.2,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143175635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}