IF 10.9 1区材料科学

Nano Today Pub Date : 2025-12-01 Epub Date: 2025-08-09 DOI: 10.1016/j.nantod.2025.102865

Yushuang Wei, Xiangyang Li, Yao Gong, Yue-Xuan Li, Jibin Guan, Bing Yuan, Yue Chen, Hong-Bo Pang

{"title":"Peroxidase-catalyzed proximity labeling to survey the proteome of nanomaterial-cell interface during macropinocytosis-mediated internalization.","authors":"Yushuang Wei, Xiangyang Li, Yao Gong, Yue-Xuan Li, Jibin Guan, Bing Yuan, Yue Chen, Hong-Bo Pang","doi":"10.1016/j.nantod.2025.102865","DOIUrl":"10.1016/j.nantod.2025.102865","url":null,"abstract":"<p><p>Nanomaterials often need to interact with proteins on the plasma membrane to get cross and access their intracellular targets. Therefore, to fully understand the cell entry mechanism, it is of vital importance to gain a comprehensive insight into the proteome at the interface when nanomaterials encounter the cells. Here, we reported a peroxidase-based proximity labeling method to survey the proteome at the nanoparticle (NP)-cell interface. Horseradish peroxidase (HRP) was conjugated to a variety of NPs and other ligand types while still being able to biotinylate the proteins surrounding NP (or ligand)-receptor complexes. Using two NP-based tracers for macropinocytosis (MP), which is highly relevant to NP internalization, we performed a proteomic survey and revealed the interface proteome difference between traditional and receptor-dependent MP. Moreover, our survey found that E-cadherin (CDH1), while not serving as the primary receptor, is present at the NP-cell interface and is functionally important for the cellular uptake of a wide variety of NPs. Overall, by integrating nanotechnology with proximity labeling, our study provides an approach to map the proteome of NP-cell interface for investigating the molecular mechanism of NP and macromolecule internalization into cells.</p>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"65 ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12410780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cancer models in nanomedicine research: Rethinking in vitro models for translational nanomedicine 纳米医学研究中的癌症模型：对转化纳米医学体外模型的再思考

IF 10.9 1区材料科学

Nano Today Pub Date : 2025-10-15 DOI: 10.1016/j.nantod.2025.102911

Lucy Wang , Oluwatomilayo Ejedenawe , Stephanie Lheureux , Danielle Rodin , Christine Allen

{"title":"Cancer models in nanomedicine research: Rethinking in vitro models for translational nanomedicine","authors":"Lucy Wang , Oluwatomilayo Ejedenawe , Stephanie Lheureux , Danielle Rodin , Christine Allen","doi":"10.1016/j.nantod.2025.102911","DOIUrl":"10.1016/j.nantod.2025.102911","url":null,"abstract":"<div><div>The U.S. FDA’s recent move to ease mandatory animal testing requirements has renewed scrutiny of in vitro models used in preclinical drug development. In nanomedicine, platforms such as spheroids, organoids, and organ-on-chip have advanced mechanistic modeling, yet the predictive validity of these systems remains limited by the biological relevance of the cell lines they incorporate. This Perspective critically evaluates the translational implications of using a narrow set of immortalized cancer cell lines in nanomedicine research – many of which lack genetic, phenotypic, or demographic alignment with cancer disease biology. Through a literature review of the 50 most cited studies in gynecologic cancer nanomedicine, we reveal an overreliance on just three cell lines in over 60–80 % of surveyed publications. We further show that most available gynecologic cancer cell lines are of European ancestry, with limited representation of global populations, despite growing evidence of population-specific differences in nanomedicine clinical efficacy and toxicity. These findings underscore a critical bottleneck in the development pipeline: the overuse of preclinical models that lack the biological variability necessary for robust clinical translation. As regulatory frameworks increasingly prioritize in vitro data in preclinical evaluation, the need to widen our cancer cell model repertoire becomes increasingly urgent. We propose actionable strategies to improve model representativeness and foster early stakeholder engagement in preclinical research. By embedding these practices into nanomedicine development, the field can strengthen translational outcomes, potentially reducing late-stage failures while better meeting the needs of the global oncology market.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"66 ","pages":"Article 102911"},"PeriodicalIF":10.9,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145321239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Layered GaPS4 dielectric for two-dimensional transistors 用于二维晶体管的层状GaPS4介电介质

IF 10.9 1区材料科学

Nano Today Pub Date : 2025-10-13 DOI: 10.1016/j.nantod.2025.102915

Liqun Niu , Zhiren Chen , Guorui Xiao , Zhaowei Zhang , Yinning Zhou , Yu Zhou , Huamin Li , Shen Lai

{"title":"Layered GaPS4 dielectric for two-dimensional transistors","authors":"Liqun Niu , Zhiren Chen , Guorui Xiao , Zhaowei Zhang , Yinning Zhou , Yu Zhou , Huamin Li , Shen Lai","doi":"10.1016/j.nantod.2025.102915","DOIUrl":"10.1016/j.nantod.2025.102915","url":null,"abstract":"<div><div>Developing van der Waals (vdW) high-k dielectric layers is a key factor in achieving high-performance two-dimensional (2D) semiconductor field effect transistors (FETs). Here, we experimentally reveal that layered GaPS<sub>4</sub> flakes exhibit a high dielectric constant of up to 35 and a high capacitance density (∼2.95 μF/cm²), along with a bandgap larger than 4.15 eV. Band alignment of MoS<sub>2</sub>/GaPS<sub>4</sub> heterostructure indicates a unipolar-like barrier between MoS<sub>2</sub> and GaPS<sub>4</sub> for electrons of ∼1.92 eV. We employed GaPS<sub>4</sub> as gate dielectric with an equivalent oxide thickness (EOT) of 1 nm in a MoS<sub>2</sub> FET, and the device shows a low gate leakage current of 10<sup>−13</sup> A, a high on/off ratio of ∼3 × 10<sup>8</sup>, and minimal hysteresis (∼20 mV). Theoretical modeling confirms that weak interactions preserve the MoS<sub>2</sub> channel’s inherent electronic properties. Compared to other layered dielectrics, GaPS<sub>4</sub> in MoS<sub>2</sub> FETs demonstrates superior properties in terms of bandgap, dielectric constant, EOT and on/off ratio. These advantages highlight the potential of GaPS<sub>4</sub> for integration into 2D semiconductor FETs.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"66 ","pages":"Article 102915"},"PeriodicalIF":10.9,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145321237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyelectrolyte nanocomplexes responsive to pathological neuronal discharge realize precise drug delivery for treating acute epilepsy via neurotransmitter homeostasis modulation 多电解质纳米复合物响应病理神经元放电，通过调节神经递质稳态，实现治疗急性癫痫的精确给药

IF 10.9 1区材料科学

Nano Today Pub Date : 2025-10-11 DOI: 10.1016/j.nantod.2025.102916

Xinrui Zhao , Rong Yang , Yage Sun , Wenguang Liu

{"title":"Polyelectrolyte nanocomplexes responsive to pathological neuronal discharge realize precise drug delivery for treating acute epilepsy via neurotransmitter homeostasis modulation","authors":"Xinrui Zhao , Rong Yang , Yage Sun , Wenguang Liu","doi":"10.1016/j.nantod.2025.102916","DOIUrl":"10.1016/j.nantod.2025.102916","url":null,"abstract":"<div><div>Abnormal neuronal discharge due to disordered neurotransmitter homeostasis and harsh inflammation in epileptic focus is prone to cause recurrent seizures. However, current difficulties in drug delivery across blood-brain barrier (BBB) and regulation of complex pathological microenvironment hinder complete control of epilepsy with clinical therapies. Herein, a novel microenvironment-responsive drug-loaded polyelectrolyte nanocomplex (HCT-Fu@VB<sub>6</sub>) formed by electrostatic self-assembly between tryptophan-modified hydroxypropyl chitosan (HCT) and fucoidan (Fu) with efficient vitamin B<sub>6</sub> (VB<sub>6</sub>) loading, is developed for potent epilepsy treating, innovatively aiming to modulate neurotransmitters balance and attenuate the neuroinflammatory responses. Specifically, HCT confers the nanoparticles with specific binding affinity towards L-type amino acid transporter 1 expressed on brain endothelial cells, thereby significantly contributing to enhanced trans-BBB drug delivery efficiency. The fucoidan-containing polyelectrolyte nanocomplexes serve to provide intrinsic antioxidant property. Notably, the prepared polyelectrolyte nanocomplexes are dissociated in response to the mimic pathological neuronal discharges due to disturbed electrostatic equilibrium, facilitating accelerated VB<sub>6</sub> release for enhanced oxidative stress mitigation and effective neurotransmitter homeostasis modulation, acting as a competitive antagonist of ATP-gated purinergic P2RX7. The developed HCT-Fu@VB<sub>6</sub> nanoparticles demonstrate significant therapeutic efficacy as an electrically responsive trans-BBB drug delivery system, remarkably reducing seizure in epileptic rats while maintaining an excellent safety profile.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"66 ","pages":"Article 102916"},"PeriodicalIF":10.9,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145321238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tailored apoptotic vesicles promote bone regeneration by stepping on osteoinductive accelerator via ITGA10-AKT signaling activation 定制的凋亡囊泡通过ITGA10-AKT信号激活，通过踩骨诱导加速器促进骨再生

IF 10.9 1区材料科学

Nano Today Pub Date : 2025-10-06 DOI: 10.1016/j.nantod.2025.102912

Yuhe Jiang , Zeying Wang , Ruiyi Guo , Yike Liao , Hao Qiu , Yunsong Liu , Ping Zhang , Yuan Zhu , Wenyue Li , Xiao Zhang , Yongsheng Zhou

{"title":"Tailored apoptotic vesicles promote bone regeneration by stepping on osteoinductive accelerator via ITGA10-AKT signaling activation","authors":"Yuhe Jiang , Zeying Wang , Ruiyi Guo , Yike Liao , Hao Qiu , Yunsong Liu , Ping Zhang , Yuan Zhu , Wenyue Li , Xiao Zhang , Yongsheng Zhou","doi":"10.1016/j.nantod.2025.102912","DOIUrl":"10.1016/j.nantod.2025.102912","url":null,"abstract":"<div><div>Apoptotic vesicles (apoVs) have shown good potential in treating bone disease including osteoporosis. However, the efficient production of highly energetic apoVs is one of the critical limitations for their clinical applications. In this study, we contributed to reconstructing a gradual senescence process in mesenchymal stem cell (MSC)-derived apoVs. Our findings displayed that their essential properties remained consistent throughout replicative aging and individual’s aging. Nevertheless, their protein composition and biological functions were significantly altered by the age of donor. Through proteomic assay, we identified integrin α 10 (ITGA10) as a highly efficient apoV osteoinductivity accelerator in both humans and mice, and have targeted it for apoV customization. On this basis, we demonstrated accelerator-based approaches to prepare amounts of ITGA10-enriched apoVs by magnetic-activated sorting and sonic loading. These engineered apoVs proved effective in ameliorating osteoprotic bone loss. Their osteoinductivity was attributed to the activation of downstream AKT signaling in recipient MSCs by the high content of ITGA10. In summary, we have laid the groundwork for customizing apoVs through an accelerator-based strategy, aiming to obtain superior therapeutic effectors in the field of bone regenerative biomedicine.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"66 ","pages":"Article 102912"},"PeriodicalIF":10.9,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Redox-driven mechanoregulation of invasive TNBC cells using poly(tannic acid) nanospheres 利用聚单宁酸纳米球对侵袭性TNBC细胞的氧化还原驱动机制调控

IF 10.9 1区材料科学

Nano Today Pub Date : 2025-10-06 DOI: 10.1016/j.nantod.2025.102907

Minhee Ku , Suhui Jeong , Nara Yoon , Hwain Myeong , Jinwon Kwon , Jaemoon Yang , Sungbaek Seo

{"title":"Redox-driven mechanoregulation of invasive TNBC cells using poly(tannic acid) nanospheres","authors":"Minhee Ku , Suhui Jeong , Nara Yoon , Hwain Myeong , Jinwon Kwon , Jaemoon Yang , Sungbaek Seo","doi":"10.1016/j.nantod.2025.102907","DOIUrl":"10.1016/j.nantod.2025.102907","url":null,"abstract":"<div><div>Poly(tannic acid) (pTA) nanospheres, assembled from natural tannic acid molecules, exhibit strong intracellular antioxidant activity and effectively modulate the invasive behaviour of triple-negative breast cancer (TNBC) cells. Acting as redox-active nanostructures, pTA nanospheres suppress proliferation and induce mechanoregulatory changes, including altered nuclear morphology, cytoskeletal disassembly, and diminished cell polarity. Specifically, pTA treatment causes spatial mislocalization of MT1-MMP from the invasive front to the perinuclear zone, disrupting its colocalization with F-actin and reducing its matrix-degrading capacity. High-resolution STED and TEM imaging reveal vimentin network collapse and mitochondrial redistribution along microtubules. Metabolic profiling shows a marked decline in oxidative phosphorylation-linked ATP production. Despite these functional disruptions, cleaved caspase-3 remains undetectable, indicating a non-apoptotic, cytostatic state accompanied by autophagy and redox signalling compensation. These findings demonstrate that pTA nanospheres exert redox-driven mechanoregulation in TNBC cells, limiting their invasive potential without inducing cell death, and highlight their promise as a non-lethal nanotherapeutics approach for post-surgical or adjuvant control of metastatic progression.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"66 ","pages":"Article 102907"},"PeriodicalIF":10.9,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinct immunological features of Ferritin and AP205 nanovaccines lead to differing therapeutic outcomes against chronic hepatitis B 铁蛋白和AP205纳米疫苗不同的免疫学特性导致对慢性乙型肝炎不同的治疗结果

IF 10.9 1区材料科学

Nano Today Pub Date : 2025-10-01 DOI: 10.1016/j.nantod.2025.102909

Xiaoxiao Zhou , Wenjun Wang , Yiyuan Zheng , Jiyu Ding , Mingzhao Zhu

{"title":"Distinct immunological features of Ferritin and AP205 nanovaccines lead to differing therapeutic outcomes against chronic hepatitis B","authors":"Xiaoxiao Zhou , Wenjun Wang , Yiyuan Zheng , Jiyu Ding , Mingzhao Zhu","doi":"10.1016/j.nantod.2025.102909","DOIUrl":"10.1016/j.nantod.2025.102909","url":null,"abstract":"<div><div>Ferritin and AP205 are two well-established representative nanoparticle platforms widely used in vaccine development. However, their immunological properties, vaccine efficacy, and underlying mechanisms remain incompletely characterized. In this study, we systematically compared the immune profiles of Ferritin- and AP205-based nanovaccines, explored their mechanisms of action, and evaluated their efficiency in the AAV-HBV infection mouse model. Our results demonstrated that the AP205 vaccine, which incorporates intrinsic ssRNA as a built-in adjuvant, elicited a stronger antibody response with a balanced IgG1/IgG2c profile. In contrast, the Ferritin vaccine supplemented with extrinsic CpG adjuvant induced an IgG1-biased antibody response. At the T cell level, the AP205 vaccine promoted a more mature germinal center T follicular helper (GC-Tfh) cell response, whereas the Ferritin+CpG vaccine stimulated a stronger Th1 response, likely due to enhanced dendritic cell activation by CpG. We further showed that codelivery of antigen and adjuvant is necessary and sufficient to augment IgG2c response for both platforms. Functionally, although the AP205-preS1 vaccine exhibited superior preventive efficacy against acute AAV-HBV infection compared to the Ferritin-preS1 +CpG vaccine, it showed reduced therapeutic efficacy against chronic AAV-HBV infection, highlighting the importance of Th1 immunity in viral clearance. Together, these findings suggest that the AP205 platform may serve as an effective platform for prophylactic vaccines, while Ferritin+CpG may hold greater potential for therapeutic applications requiring strong Th1 responses, such as chronic hepatitis B (CHB).</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"66 ","pages":"Article 102909"},"PeriodicalIF":10.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-dimensional ferromagnetism revealed by Kondo effect and linear V/W-shaped anisotropic magnetoresistance 近藤效应揭示的一维铁磁性和线性V/ w型各向异性磁阻

IF 10.9 1区材料科学

Nano Today Pub Date : 2025-10-01 DOI: 10.1016/j.nantod.2025.102908

Zheng Wei , Yu-Hao Wan , Wenxiang Wang , Jiawang You , Zhisheng Peng , Julienne Impundu , Tao He , Changzhi Gu , Hongxuan Ren , Yong Jun Li , Qing-Feng Sun , Lianfeng Sun

{"title":"One-dimensional ferromagnetism revealed by Kondo effect and linear V/W-shaped anisotropic magnetoresistance","authors":"Zheng Wei , Yu-Hao Wan , Wenxiang Wang , Jiawang You , Zhisheng Peng , Julienne Impundu , Tao He , Changzhi Gu , Hongxuan Ren , Yong Jun Li , Qing-Feng Sun , Lianfeng Sun","doi":"10.1016/j.nantod.2025.102908","DOIUrl":"10.1016/j.nantod.2025.102908","url":null,"abstract":"<div><div>Magnetic anisotropy can remove Mermin-Wagner prohibitions for magnetic order in two-dimensional materials. An interesting and fundamental question is to explore magnetism in materials/structures with still lower dimensions. Here we show that there is one-dimensional ferromagnetism along the edges between a molybdenum (Mo) strip and a monolayer graphene (MLG). When a Mo strip is deposited across an MLG with magnetron sputtering, the MLG underneath Mo is removed due to a solid-solid reaction, making two newly-formed, one-dimensional edges between the Mo strip and the MLG. For one Mo strip on an MLG, Kondo effect and anisotropic magnetoresistance (AMR) can be observed, which exhibits a unique linear V/W-shape in small magnetic fields. For two parallel Mo strips on a single MLG, besides Kondo effect and AMR, spin Hall effect (SHE) and inverse spin Hall effect (ISHE) have been observed at temperature up to 300 K, which implies the antiferromagnetic coupling between the edges. These results indicate the coexistence of Kondo effect and one-dimensional ferromagnetism and great potential applications.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"66 ","pages":"Article 102908"},"PeriodicalIF":10.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revolutionizing role of magnetic field-guided MnFe2O4@ZIF-8@retinoic acid in DUCA conduits for inflammation inhibition and peripheral nerve regeneration 革命性的作用磁场引导MnFe2O4@ZIF-8@维甲酸在DUCA导管炎症抑制和周围神经再生

IF 10.9 1区材料科学

Nano Today Pub Date : 2025-09-30 DOI: 10.1016/j.nantod.2025.102910

Majid Sharifi , Majid Salehi , Somayeh Ebrahimi-Barough , Mohammad Kamalabadi-Farahani

{"title":"Revolutionizing role of magnetic field-guided MnFe2O4@ZIF-8@retinoic acid in DUCA conduits for inflammation inhibition and peripheral nerve regeneration","authors":"Majid Sharifi , Majid Salehi , Somayeh Ebrahimi-Barough , Mohammad Kamalabadi-Farahani","doi":"10.1016/j.nantod.2025.102910","DOIUrl":"10.1016/j.nantod.2025.102910","url":null,"abstract":"<div><div>Neuroma formation following the repair of nerve injuries exceeding 0.9 cm in length severely impedes functional recovery. To overcome this challenge, we engineered magnetically responsive core-shell nanoparticles (MnFe<sub>2</sub>O<sub>4</sub>@ZIF-8@Retinoic acid, MFZR; 90–360 nm) to guide regeneration within a decellularized umbilical cord artery (DUCA) conduit. We investigated the synergistic effect of MFZR under an external magnetic field (MF) on Schwann cell behavior in vitro and on sciatic nerve repair in a rat model. Under MF exposure, MFZR significantly enhanced Schwann cell migration, alignment, and elongation on DUCA conduits. <em>In-vivo</em>, the MFZR+MF combination potently promoted functional recovery, as measured by the sciatic functional index, muscle compound action potential, and nerve conduction velocity, without inducing DUCA-related inflammation. Histological analysis demonstrated robust regeneration, characterized by increased axon diameter, an improved G-ratio, and elevated expression of S100 and NF-200. This regeneration was facilitated by a healing-promoting M2 macrophage polarization at the injury site. Critically, the strategy exhibited no systemic toxicity. These findings establish that magnetically guided MFZR effectively prevents neuroma in the DUCA conduits, orchestrates a pro-regenerative microenvironment, and achieves significant functional recovery, offering a promising translatable strategy for nerve repair.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"66 ","pages":"Article 102910"},"PeriodicalIF":10.9,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-invasive CRISPR/Cas9 nanocapsules specifically edit α-synuclein for effective Parkinson’s disease treatment 非侵入性CRISPR/Cas9纳米胶囊特异性编辑α-突触核蛋白，有效治疗帕金森病

IF 10.9 1区材料科学

Nano Today Pub Date : 2025-09-30 DOI: 10.1016/j.nantod.2025.102903

Qingshan Yang , Yujing Sang , Nan Geng , Yang Liu , Dongya Zhang , Yan Zou , Meng Zheng

{"title":"Non-invasive CRISPR/Cas9 nanocapsules specifically edit α-synuclein for effective Parkinson’s disease treatment","authors":"Qingshan Yang , Yujing Sang , Nan Geng , Yang Liu , Dongya Zhang , Yan Zou , Meng Zheng","doi":"10.1016/j.nantod.2025.102903","DOIUrl":"10.1016/j.nantod.2025.102903","url":null,"abstract":"<div><div>Parkinson’s disease (PD) is the most common movement disorders, affecting more than 1 % of the elderly population aged over 60 years old. Targeting the accumulation of the toxic protein α-synuclein (α-Syn) (<em>SNCA</em>) is a common therapeutic strategy for PD. CRISPR/Cas9 gene technology could provide an avenue to achieve reduced levels of this protein. However, the lack of effective and safe brain delivery vectors greatly hampers its applications for brain disorders. In this paper, we developed glucose directed single-particle nanocapsules that efficiently delivers CRISPR/Cas9 into targeted brain lesions to specifically edit the <em>SNCA</em> gene. Our CRISPR/Cas9 nanocapsules have a small size of 32 nm and formed with a polymeric shell which protects Cas9/sgRNA from enzymatic degradation. Benefitting from surface glucose decoration, our nanocapsules exhibited blood brain barrier (BBB) permeability and accumulation in brain lesions after intravenous administration. Additionally, CRISPR/Cas9 nanocapsules selectively reduced expression of the <em>SNCA</em> leading to down regulation of α-Syn protein, M1/M2 microglial re-polarization, amelioration of neuroinflammation and recovery of tryptophan hydroxylase (TH) in A53T transgenic mice. Importantly, CRISPR/Cas9 nanocapsules significantly improved performance of mice in a variety behavioral test with negligible side effects. Therefore, the CRISPR/Cas9 nanocapsules provides a versatile but potent platform for genetic engineering in brain disorders, especially genome mutations relevant to neuronal disease.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"66 ","pages":"Article 102903"},"PeriodicalIF":10.9,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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