IF 10.9 1区材料科学

Nano Today Pub Date : 2026-04-01 Epub Date: 2026-02-03 DOI: 10.1016/j.nantod.2026.102996

Jiarong Li , Yonghui Li , Zhen Wan , Hongyang Li , Haitao Dai , Changlong Liu , Xiaoyu Mu , Xiao-Dong Zhang

{"title":"Spin-mediated and proton-coupled electron transfer boost NADH oxidase-like activity of dual-atom metallozymes","authors":"Jiarong Li , Yonghui Li , Zhen Wan , Hongyang Li , Haitao Dai , Changlong Liu , Xiaoyu Mu , Xiao-Dong Zhang","doi":"10.1016/j.nantod.2026.102996","DOIUrl":"10.1016/j.nantod.2026.102996","url":null,"abstract":"<div><div>Nicotinamide adenine dinucleotide (NAD<sup>+</sup>) as a pivotal coenzyme plays a central role in the pathogenesis of various inflammatory diseases. However, both natural and artificial NADH oxidase (NOX) systems exhibit limited efficacy in NAD<sup>+</sup> regeneration. Here, we developed a RuCo heterogeneous dual atom metallozymes (DAMs) with over-natural NOX activity through spin-mediated and proton-coupled electron transfer (PCET). Under simulated inflammatory microenvironment, DAMs exhibited a catalytic efficiency (<em>K</em><sub><em>cat</em></sub><em>/K</em><sub><em>m</em></sub>) of 0.99 μM<sup>−1</sup>min<sup>−1</sup>, surpassing reported artificial enzymes by ∼9–99 times. Additionally, its quantitative NOX activity reached 2139 U/mg, about 6–18 times that of common microbial enzymes. The experimental and computational studies revealed that Co not only modulated the spin state of Ru from low-spin to middle-spin, but also its electrophilic microenvironment facilitated oxygen adsorption to further modulate electronic behavior of Ru with high-spin. Thus, Ru-Co-O as high active sites preferred to trap electron and proton from NADH and acidic conditions via a PECT mechanism. In a skin injury model, DAMs alleviated inflammation and promoted skin regeneration by restoring the NAD<sup>+</sup>/NADH ratio, enhancing energy metabolism, and driving macrophage anti-inflammatory polarization. Notably, no relapse was observed within 20 days post-treatment, highlighting the long-lasting therapeutic impact of DAMs.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"68 ","pages":"Article 102996"},"PeriodicalIF":10.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146171229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorinated amphipathic cationic peptides for intracellular protein delivery and cancer immunotherapy 用于细胞内蛋白传递和癌症免疫治疗的氟化两性阳离子肽

IF 10.9 1区材料科学

Nano Today Pub Date : 2026-04-01 Epub Date: 2026-01-24 DOI: 10.1016/j.nantod.2026.102979

Wenhua Cheng , Yan Lou , Yiyun Cheng , Xin Gao , Quan Huang

{"title":"Fluorinated amphipathic cationic peptides for intracellular protein delivery and cancer immunotherapy","authors":"Wenhua Cheng , Yan Lou , Yiyun Cheng , Xin Gao , Quan Huang","doi":"10.1016/j.nantod.2026.102979","DOIUrl":"10.1016/j.nantod.2026.102979","url":null,"abstract":"<div><div>Cell penetrating peptides are widely developed as carriers to transport proteins inside cells, but there remain some drawbacks such as limited protein loading capacity and nanoparticle stability, endosomal escape and intracellular delivery efficacy. Herein, we design a family of fluorinated amphipathic cationic peptides (FACPs) to address these issues. A library of amphipathic cationic hexapeptides is conjugated with a fluorous tag at the <em>C</em>-terminus via a dynamic hydrazone bond. Among the investigated materials, FACP2 (peptide sequence: RRRWWW) demonstrates the optimal performance in protein binding, cellular uptake, endosomal escape, and thus intracellular delivery efficacy. It efficiently delivers ovalbumin into bone marrow derived dendritic cells and stimulates their maturation, enhancing the antigen cross-presentation both <em>in vitro</em> and <em>in vivo</em>. As a result, the FACP2/ovalbumin nanovaccine effectively inhibits the tumor growth in mice when combined with anti-PD1 therapy or co-delivered with the STING agonist 2′,3′-cyclic guanosine monophosphate. This study provides a highly efficient peptide nanocarrier for cytosolic protein delivery and cancer immunotherapy through antigen and adjuvant co-delivery.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"68 ","pages":"Article 102979"},"PeriodicalIF":10.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeted isolation of astrocyte-derived extracellular vesicles using peptide-imprinted nanocomposites for neurological diagnostics 利用肽印迹纳米复合材料靶向分离星形胶质细胞来源的细胞外囊泡用于神经诊断

IF 10.9 1区材料科学

Nano Today Pub Date : 2026-04-01 Epub Date: 2026-02-06 DOI: 10.1016/j.nantod.2026.103001

Hyo Joo Lee , Yeonjeong Roh , Thuy Nguyen Thi Dao , Jin Hee Kim , Wangyong Shin , Dayoung Seo , Bonhan Koo , Eun-Jae Lee , Yong Shin

{"title":"Targeted isolation of astrocyte-derived extracellular vesicles using peptide-imprinted nanocomposites for neurological diagnostics","authors":"Hyo Joo Lee , Yeonjeong Roh , Thuy Nguyen Thi Dao , Jin Hee Kim , Wangyong Shin , Dayoung Seo , Bonhan Koo , Eun-Jae Lee , Yong Shin","doi":"10.1016/j.nantod.2026.103001","DOIUrl":"10.1016/j.nantod.2026.103001","url":null,"abstract":"<div><div>Neurological disorders require early detection, yet traditional diagnostic methods lack specificity and sensitivity. Blood-derived extracellular vesicles (EVs) provide a promising, non-invasive alternative for biomarker discovery in neurological disorders, despite challenges in cell-specific isolating them from brain related EVs due to their complexity and scarcity. However, the heterogeneity and low yield of EVs, common EVs isolation methods remain challenges, then the development of more efficient and accurate isolation approach. This study presents Engineering Peptide-Imprinted Nanocomposites (EPIN) as an innovative platform for isolating cell-specific EVs from biological matrices, overcoming the limitations of conventional methods. Utilizing magnetic nanoparticles, EPIN is developed with peptide template that bind specifically to EVs surface proteins, enhancing the capture efficiency. Targeting proteins CD63, CD9, and CD81, EPIN shows high specificity in isolating EVs, compared with current existing assays. In particular, EPIN-glutamate aspartate transporter (GLAST) effectively isolates astrocyte-derived EVs in blood, indicating significant promise for monitoring neuromyelitis optica spectrum disorder (NMOSD), where astrocytopathy is a key feature. The utility of EPIN-GLAST is demonstrated using 147 blood samples by integrating brain-derived proteins and miRNAs, establishing a platform for disease-specific monitoring. This approach extends beyond detecting astrocytic damage in NMOSD, representing a significant advancement in EV-based diagnostics. These findings underscore the capability of EPIN-GLAST in elucidating disease dynamics and emphasize the value of targeted EVs analysis in neurological disorder research, enabling novel biomarker discovery.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"68 ","pages":"Article 103001"},"PeriodicalIF":10.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146171232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carrier-free self-assembly of choline chloride-ammonium glycyrrhizinate hydrogel for myocardial infarction microenvironment regulation 无载体自组装氯化胆碱-甘草酸铵水凝胶对心肌梗死微环境的调节

IF 10.9 1区材料科学

Nano Today Pub Date : 2026-04-01 Epub Date: 2026-02-26 DOI: 10.1016/j.nantod.2026.103014

Yi Hu , Wenhui Yang , Jiamian Zhan , Yue Ma , Pengqi Wang , Chunyi Pu , Linfang Zhong , Xiaozhong Qiu , Honghao Hou

{"title":"Carrier-free self-assembly of choline chloride-ammonium glycyrrhizinate hydrogel for myocardial infarction microenvironment regulation","authors":"Yi Hu , Wenhui Yang , Jiamian Zhan , Yue Ma , Pengqi Wang , Chunyi Pu , Linfang Zhong , Xiaozhong Qiu , Honghao Hou","doi":"10.1016/j.nantod.2026.103014","DOIUrl":"10.1016/j.nantod.2026.103014","url":null,"abstract":"<div><div>Myocardial infarction (MI)-induced persistent disruption of the myocardial microenvironment is one of the key mechanisms driving the onset of heart failure. Precise modulation of this pathological microenvironment <em>in vitro</em> has emerged as an important strategy to improve MI prognosis and delay heart failure progression. In this study, we constructed carrier‑free choline chloride (ChCl)–ammonium glycyrrhizinate (AG) self‑assembled hydrogels. Initially, network pharmacology combined with big data analysis was employed to predict the synergistic therapeutic effects of ChCl and AG. Subsequently, multi‑scale characterisation techniques, including FT-IR, SEM, and rheology, were used to analyse the structural features of the gel system. Molecular dynamics and quantum chemical calculations indicated that hydrophobic, electrostatic, and hydrogen‑bond interactions jointly drive self‑assembly, and Cryo‑SEM revealed for the first time a near‑native interpenetrating sheet‑like fibrous network. In an <em>in vitro</em> MI model, the ChCl-AG self-assembled hydrogel reduced oxidative stress, shifted macrophages from pro‑inflammatory M1 to reparative M2, increased endogenous antioxidant enzyme activity, and downregulated pro‑inflammatory cytokines, thereby preserving microenvironmental homeostasis and providing cardioprotection. Transcriptomics indicated positive regulation of pathways linked to cardiac structure, contractility, energy metabolism, and cardiovascular disease. <em>In vivo</em> MI models, the ChCl-AG self-assembled hydrogel markedly promoted myocardial repair and functional recovery while exhibiting good biocompatibility and sustained release. Overall, the carrier‑free ChCl-AG self-assembled hydrogel provides a precise strategy to modulate post‑MI inflammatory and oxidative imbalances, promote myocardial repair, and holds broader biomedical potential.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"68 ","pages":"Article 103014"},"PeriodicalIF":10.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147381601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular architectonics of semiconducting polymer dots for next-generation NIR-II fluorescence bioimaging 用于下一代NIR-II荧光生物成像的半导体聚合物点的分子结构

IF 10.9 1区材料科学

Nano Today Pub Date : 2026-04-01 Epub Date: 2026-02-28 DOI: 10.1016/j.nantod.2026.103016

Nabil Omri, Vignesh Kumaravel

{"title":"Molecular architectonics of semiconducting polymer dots for next-generation NIR-II fluorescence bioimaging","authors":"Nabil Omri, Vignesh Kumaravel","doi":"10.1016/j.nantod.2026.103016","DOIUrl":"10.1016/j.nantod.2026.103016","url":null,"abstract":"<div><div>Next-generation semiconducting polymer dots (Pdots) engineered through molecular architectonics are redefining NIR-II fluorescence bioimaging and precision theranostics. By integrating donor-acceptor engineering, π-conjugated backbone rigidification, and side-chain modulation, molecular architectonics enables precise bandgap tuning, exciton delocalization, and enhanced photochemical stability. Architectonic control across molecular, supramolecular, and interfacial levels yields Pdots with superior quantum yields, ultradeep tissue penetration, and negligible autofluorescence. This review elucidates the structure-function paradigms governing NIR-II emission and examines frontier strategies for photostability enhancement, including nanoscale confinement within hybrid matrices, intersystem energy dissipation modulation, and conformational locking. At the biointerface, rational surface architectonics, via dynamic ligand exchange, stealth polymer cloaking, and adaptive functionalization, prolongs circulation, minimizes opsonization, and facilitates targeted delivery. Integrating NIR-II fluorescence with photothermal, photodynamic, and image-guided drug delivery modalities, Pdots emerge as molecularly architected cores for multimodal theranostic systems synergized with photoacoustic and magnetic resonance imaging. Despite rapid progress, biodegradability, long-term biosafety, and regulatory compatibility remain major challenges. Emerging architectonic solutions, bioresponsive degradation and hybridization with clinically approved carriers, are bridging molecular design and clinical translation, positioning NIR-II Pdots as key enablers of next-generation intelligent nanomedicine.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"68 ","pages":"Article 103016"},"PeriodicalIF":10.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147385845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound-activated pyroptosis using silk microneedle patch for cancer immunotherapy 丝质微针贴片在肿瘤免疫治疗中的应用

IF 10.9 1区材料科学

Nano Today Pub Date : 2026-04-01 Epub Date: 2026-02-24 DOI: 10.1016/j.nantod.2026.103010

Ming Zhang , Yifan Li , Hongyu Tang , Mingxuan Li , Meifang Shen , Tiejun Bing , Yingjie Yu , Yushu Wang , Wenhao Hu

{"title":"Ultrasound-activated pyroptosis using silk microneedle patch for cancer immunotherapy","authors":"Ming Zhang , Yifan Li , Hongyu Tang , Mingxuan Li , Meifang Shen , Tiejun Bing , Yingjie Yu , Yushu Wang , Wenhao Hu","doi":"10.1016/j.nantod.2026.103010","DOIUrl":"10.1016/j.nantod.2026.103010","url":null,"abstract":"<div><div>Cancer immunotherapy is limited by the immunosuppressive tumor microenvironment, which restricts immune activation and limits therapeutic efficacy. Pyroptosis, a pro-inflammatory form of programmed cell death mediated by Caspase-dependent gasdermin cleavage, has emerged as an effective strategy for remodeling the tumor microenvironment and promoting antitumor immunity. Reactive oxygen species (ROS) can activate the Caspase-3/GSDME pathway to induce pyroptosis, offering spatiotemporal control over immune activation. Among ROS-based approaches, sonodynamic therapy (SDT) provides key advantages, including deep tissue penetration, noninvasive ultrasound (US) activation, and efficient ROS generation. In this study, an ultrathin 2D Cu-TCPP nanosheet (CuT) is developed as a high-performance sonosensitizer, with enhanced oxygen interaction and electron transfer for amplified ROS generation under US irradiation. To improve intratumoral delivery and retention, CuT is further integrated into silk fibroin-based microneedles (CuT-MN) for localized, minimally invasive administration. This platform enables precise tumor targeting, sustained sonosensitizer enrichment, and on-demand pyroptosis induction, while minimizing off-target toxicity. Overall, the CuT-MN platform effectively and enhances antitumor immune responses, representing a promising approach for precision sonodynamic immunotherapy.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"68 ","pages":"Article 103010"},"PeriodicalIF":10.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147385850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the size and surface effects of spinel Co3O4 on the activity of oxygen evolution 揭示了尖晶石Co3O4的尺寸和表面对析氧活性的影响

IF 10.9 1区材料科学

Nano Today Pub Date : 2026-04-01 Epub Date: 2026-02-12 DOI: 10.1016/j.nantod.2026.103004

Ce Zhao , Zhou Jiang , Bing Zhang , Zhangqiang Li , Xuanping Zhou , Liuyang Xiao , Wei Zhang , Yong Zhang

{"title":"Unveiling the size and surface effects of spinel Co3O4 on the activity of oxygen evolution","authors":"Ce Zhao , Zhou Jiang , Bing Zhang , Zhangqiang Li , Xuanping Zhou , Liuyang Xiao , Wei Zhang , Yong Zhang","doi":"10.1016/j.nantod.2026.103004","DOIUrl":"10.1016/j.nantod.2026.103004","url":null,"abstract":"<div><div>Size control is a facile and effective strategy to design high-performance electrocatalysts of oxygen evolution reaction (OER) but remains challenging, due to the insufficient understanding of size-dependent catalytic activity of electrocatalysts like spinel cobalt oxide (Co<sub>3</sub>O<sub>4</sub>). Here, we report a top-down method to produce Co<sub>3</sub>O<sub>4</sub> nanoparticles (NPs) and quantum dots (QDs) with sizes ranging from 27.5 nm to 2.8 nm, enabling a precise investigation of the size effect on OER activity. Our results show that while the OER performance increases with smaller size due to enhanced surface area, only the smallest Co<sub>3</sub>O<sub>4</sub> QDs exhibit a significant improvement in intrinsic activity. Such QDs display notable size-reduction-induced lattice expansion along with increased Co−O bonding length, which can facilitate intermediate adsorption and contribute to their superior catalytic performance. Building on this enhanced intrinsic activity, we demonstrate that surface hydroxylation of Co<sub>3</sub>O<sub>4</sub>-OH QDs further enables an impressive overpotential of 293 mV at 10 mA cm<sub>geo</sub><sup>−2</sup> and remarkable stability over 240 h. Theoretical calculations reveal that size reduction and surface hydroxylation synergistically optimize the electronic structure, lowering the energy barrier for OER process. This work provides a practical strategy in designing efficient non-noble catalysts beyond conventional size limitations.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"68 ","pages":"Article 103004"},"PeriodicalIF":10.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146171233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ZnO nanoparticle-educated exosomes inhibit osteosarcoma metastasis through HIF-1α/miR-1287–5p/Snail axis ZnO纳米颗粒外泌体通过HIF-1α/ miR-1287-5p /蜗牛轴抑制骨肉瘤转移

IF 10.9 1区材料科学

Nano Today Pub Date : 2026-04-01 Epub Date: 2026-02-21 DOI: 10.1016/j.nantod.2026.103008

Guangping He , Zhengyuan Huang , Yuru Zong , Yong Hai , Jiayu Zhang , Jun Chen , Hanqing Chen

{"title":"ZnO nanoparticle-educated exosomes inhibit osteosarcoma metastasis through HIF-1α/miR-1287–5p/Snail axis","authors":"Guangping He , Zhengyuan Huang , Yuru Zong , Yong Hai , Jiayu Zhang , Jun Chen , Hanqing Chen","doi":"10.1016/j.nantod.2026.103008","DOIUrl":"10.1016/j.nantod.2026.103008","url":null,"abstract":"<div><div>Osteosarcoma (OS) remains the most common primary bone malignancy in children and adolescents. Despite advances in neoadjuvant chemotherapy, metastasis and recurrence constitute major clinical challenges. Zinc oxide nanoparticles (ZnO NPs) possess promising anticancer activity, yet their anti‑metastatic mechanisms in OS remain unclear. Here, we reported a novel bio-inspired strategy using exosomes derived from ZnO NP‑treated OS cells (ZnO‑Exos), that significantly suppressed cell viability, migration, and invasion in OS cells. Mechanistically, ZnO-Exos-derived Zn<sup>2 +</sup> ions activated and stabilized hypoxia-inducible factor-1α (HIF-1α), which transcriptionally upregulated the expression of exosomal miR-1287–5p, and then in turn directly targeted and downregulated the mRNA of Snail, a master transcriptional repressor driving epithelial-mesenchymal transition (EMT). In an orthotopic OS mouse model, intratumoral injection of ZnO-Exos significantly inhibited primary tumor growth and spontaneous lung metastasis, extended survival, and exhibited excellent biocompatibility. Collectively, this study elucidated a novel ZnO NP-educated exosomes that exert anti‑metastatic effects, offering a targeted, low‑toxicity strategy for OS therapy and metastasis inhibition through Zn<sup>2+</sup>/HIF-1α/miR-1287–5p/Snail axis.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"68 ","pages":"Article 103008"},"PeriodicalIF":10.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147385846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptide-specific nanoprobes for matrix-free mass spectrometric imaging of multiplexed proteins and their distributions on cells or tissues 多肽特异性纳米探针用于多路蛋白及其在细胞或组织中的分布的无基质质谱成像

IF 10.9 1区材料科学

Nano Today Pub Date : 2026-04-01 Epub Date: 2026-02-23 DOI: 10.1016/j.nantod.2026.103013

Jiahui Sun, Nan Feng, Si Zhang, Mengchen Wang, Huangxian Ju

{"title":"Peptide-specific nanoprobes for matrix-free mass spectrometric imaging of multiplexed proteins and their distributions on cells or tissues","authors":"Jiahui Sun, Nan Feng, Si Zhang, Mengchen Wang, Huangxian Ju","doi":"10.1016/j.nantod.2026.103013","DOIUrl":"10.1016/j.nantod.2026.103013","url":null,"abstract":"<div><div><em>In situ</em> detection of multiplexed proteins is greatly constrained by targeted recognition and operational process. Here we design a one-pot method to synthesize several peptide-specific nanoprobes (NPs) for quickly recognizing the targeted protein molecules, and present a matrix-free laser desorption/ionization mass spectrometric imaging (LDI-MSI) strategy for simple <em>in situ</em> imaging of multiplexed proteins on cells or tissues with amplified signals. Unlike conventional citrate-synthesized and post-modified gold nanoparticles, the resulting NPs demonstrate their simple preparation, high resolution and low background with controllable matrix-free and ion-friendly properties. These NPs have been used to conveniently and sensitively detect the relative expression of three specific proteins on four cancer cell lines and efficiently evaluate the suppression of different drugs on these proteins. The proposed MSI method possesses powerful ability to distinctly distinguish different pathological regions of tumor tissues by the change of multiplexed protein distributions, indicating its excellent promise in clinical disease diagnosis and precision medicine.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"68 ","pages":"Article 103013"},"PeriodicalIF":10.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147385848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecularly imprinted nanoreactors: Bridging enzyme mimicry and synthetic catalysis 分子印迹纳米反应器：桥接酶模拟和合成催化

IF 10.9 1区材料科学

Nano Today Pub Date : 2026-04-01 Epub Date: 2026-02-07 DOI: 10.1016/j.nantod.2026.103002

Ya Liu , Yuanyuan Cheng , Yi Hao , Xuemeng Tian , Yue Wang , Xueyi Liu , Ruixia Gao

{"title":"Molecularly imprinted nanoreactors: Bridging enzyme mimicry and synthetic catalysis","authors":"Ya Liu , Yuanyuan Cheng , Yi Hao , Xuemeng Tian , Yue Wang , Xueyi Liu , Ruixia Gao","doi":"10.1016/j.nantod.2026.103002","DOIUrl":"10.1016/j.nantod.2026.103002","url":null,"abstract":"<div><div>Molecularly imprinted nanoreactors (MIRs) represent a pivotal advance in biomimetic catalysis, offering robust and tunable alternatives to natural enzymes, yet their rational design remains a central challenge due to persistent issues such as binding-site heterogeneity, mass-transfer constraints, and limited catalytic scope. We systematically elucidate the synergistic design principle that integrates precise active-site engineering—via coordination-driven anchoring, post-imprinting modification, and spatial encapsulation—with deliberate catalytic microenvironment programming, encompassing cofactor regulation, tandem catalysis, nanoconfinement, and electronic effects. This combined approach is essential for emulating enzymatic efficiency and selectivity. Furthermore, we contrast key synthetic strategies from a “nanoreactor engineering” perspective, critically analyzing the merits and constraints of spatial encapsulation and compartmentalized designs in mimicking enzymatic cascades. Beyond model reactions, the review provides a critical assessment of MIR performance in advanced applications, including the degradation of micropollutants in environmental matrices, precision biosensing in complex media, and chiral synthesis under sustainable conditions, while honestly addressing the translation gaps between idealized systems and real-world operational demands. Finally, we propose that the convergence of MIRs with single-atom catalysts, <em>in situ</em>/<em>operando</em> spectroscopy, and synthetic biology interfaces constitutes an emerging paradigm. This integration is poised to unlock unprecedented catalytic precision, stability, and programmability, steering MIRs from laboratory toward practical deployment in green chemistry, environmental technology, and programmable biomedicine.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"68 ","pages":"Article 103002"},"PeriodicalIF":10.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146171171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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