Wiley最新文献

{"title":"Integrating Security Controls in DevSecOps: Challenges, Solutions, and Future Research Directions","authors":"Maysa Sinan,&nbsp;Mojtaba Shahin,&nbsp;Iqbal Gondal","doi":"10.1002/smr.70029","DOIUrl":"https://doi.org/10.1002/smr.70029","url":null,"abstract":"<p>Cybersecurity has become a top priority for most organizations to protect their applications. The rapid increase in cyberattacks has necessitated a comprehensive repositioning of how security should be implemented within the software development lifecycle (SDLC). Development, Security, Operations (DevSecOps) is one of the trendy security methodologies and fastest growing development methods promoting shared responsibility for security and automating security practices at every step of the SDLC. DevSecOps is a cultural shift that integrates security controls into DevOps pipelines aiming to upscale overall security. Therefore, many organizations started to incorporate security controls within the deployment of DevSecOps through conducting continuous practices, for example, automated security testing, infrastructure as code (IaC), compliance as code, and continuous monitoring. This study aims to organize the knowledge and shed light on challenges concerning security controls during the adoption of DevSecOps, along with associated solutions and remediation workarounds reported in the literature. Further, the study aims to provide clear insights into the areas that require further investigation and research in the future. A systematic literature review (SLR) of 45 primary studies was carried out to extract data, and subsequently, the extracted data was analyzed using the thematic analysis method. This paper identifies 19 challenges related to security controls that could be experienced by security practitioners while implementing a DevSecOps model, along with 18 solutions and remediation actions suggested in literature to address and overcome some of the enlisted challenges. In addition, some gap areas are identified as opportunities for future research in this domain with the aim of improving the integration of security controls in a DevSecOps environment. Based on findings, this paper points out the importance of automation in software engineering practices, for example, continuous automation, continuous delivery, and continuous feedback, to embed security controls at the early stages of the development process.</p>","PeriodicalId":48898,"journal":{"name":"Journal of Software-Evolution and Process","volume":"37 6","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/smr.70029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"计算机科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimal Hybrid Fault Tolerant Control With Voltage Balancing for IPOS DAB Converters","authors":"Chong Zhang,&nbsp;Jie Zhu,&nbsp;Xiaogang Ding,&nbsp;Samson Shenglong Yu,&nbsp;Dongsheng Yu","doi":"10.1049/pel2.70062","DOIUrl":"https://doi.org/10.1049/pel2.70062","url":null,"abstract":"<p>The input parallel output series connected dual active bridge (DAB) converter is a typical galvanically isolated converter for a wide output voltage range in many applications, such as charging piles for electric vehicles or solar energy storage systems. Open circuit fault (OCF) is an operation accident of power switches that occurs in DAB converters, and the faulty DAB module is degraded to a semi-DAB converter. In this work, by analyzing three operation modes and the impact of the current stress/power ratio, an optimal hybrid fault-tolerant control strategy is proposed. In this control method, DAB converters are operated under zero voltage switching/zero current switching under minimum inductor current stress without DC bias current. The proposed OCF fault-tolerant control strategy helps the DAB converter maintain stable operation under OCF occurrences. A voltage balancing method considering OCF and feedback-loop competition is also designed to balance the output voltages of faulty and non-faulty. Prototype experimental results verify the correctness and effectiveness of the proposed control strategy.</p>","PeriodicalId":56302,"journal":{"name":"IET Power Electronics","volume":"18 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/pel2.70062","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Opportunities and Challenges of Microalgal Strain Development via Atmospheric and Room-Temperature Plasma (ARTP)","authors":"Jo-Chi Hung,&nbsp;Priskila A. Diankristanti,&nbsp;Jo-Shu Chang,&nbsp;I-Son Ng","doi":"10.1002/biot.70049","DOIUrl":"https://doi.org/10.1002/biot.70049","url":null,"abstract":"<div>\u0000 \u0000 <p>Enhancing microalgal strains for biofuels and bioproducts often relies on genetic modification, raising concerns over regulatory and public acceptance. As a non-GMO alternative, atmospheric and room-temperature plasma (ARTP) mutagenesis has emerged as a promising tool for strain development. This review explores ARTP potential, starting with operational principles and key parameters. Special emphasis is placed on lethality control and optimized screening conditions. Several widely-studied strains are covered, including <i>Desmodesmus</i> sp., <i>Haematococcus lacustris</i>, and <i>Chlorella sorokiniana</i>. Discussion then shifts into the microscopic world to explore differentially expressed genes (DEGs), tracing CO₂ assimilation and metabolic pathways that drive accelerated growth. Four key areas are assessed in detail, spanning from photosynthetic carbon fixation, central carbon metabolism, alongside lipid and pigment biosynthesis. Major challenges are laid bare, from genetic stability all the way to reproducibility. Addressing challenges is essential to fully harness ARTP for microalgal biotechnology and extended application in sustainability.</p>\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Novel Annexin A5 Probes and a Fluorometric Microplate-Reader Method for High-Throughput Apoptosis Detection","authors":"Mengyue Gao,&nbsp;Shihui Wang,&nbsp;Yunke Wang,&nbsp;Minjin Hu,&nbsp;Sheng Wang,&nbsp;Zichun Hua","doi":"10.1002/biot.70054","DOIUrl":"https://doi.org/10.1002/biot.70054","url":null,"abstract":"<div>\u0000 \u0000 <p>Annexin A5 (AnxA5) probes have been extensively utilized to investigate apoptotic cell death, which is crucial to various fields of biological research and clinical applications. Chemically labeled fluorescent AnxA5 has been widely used, but there is a notable scarcity of blue-emitting AnxA5 probes based on fluorescent proteins (FPs). Furthermore, methods utilizing AnxA5 predominantly rely on flow cytometry or fluorescence microscopy, both of which have inherent limitations, such as the necessity of costly instruments and specialized expertise, as well as the challenge of analyzing multiple samples. Fewer studies have focused on establishing a fluorometric microplate reader method for apoptosis detection. Herein, we reported a series of novel blue-emitting AnxA5 probes, ingeniously designed by fusing FPs. These probes can be applied for detecting apoptosis with flow cytometry and fluorescence microscopy. Additionally, based on our findings regarding the strong linearity between the fluorescence intensity and concentrations of AnxA5 probes, along with their analytical performances in apoptotic samples, we determined that blue-emitting AnxA5 probes were suitable for the microplate reader assay. We highlighted the reliability and practicality of this method by comparing with results obtained from flow cytometry. This fluorometric microplate reader assay offers a promising and high-throughput strategy for cancer researchers to evaluate the pro-apoptotic effects of potential antineoplastic drugs.</p>\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alleviating Substrate Inhibition of Leucine Dehydrogenase by Enhancing NAD+ Dissociation Efficiency","authors":"Jun-Ping Zhou,&nbsp;Yi-Nan Xue,&nbsp;Feng Wang,&nbsp;Hui Gao,&nbsp;Zhi-Cheng Zhang,&nbsp;Ai-Ping Pang,&nbsp;Zhi-Qiang Liu,&nbsp;Yu-Guo Zheng","doi":"10.1002/biot.70045","DOIUrl":"https://doi.org/10.1002/biot.70045","url":null,"abstract":"<div>\u0000 \u0000 <p>Leucine dehydrogenase catalyzes the asymmetric reductive amination of ketoacids to produce valuable chiral unnatural amino acids, but its application is often constrained by substrate inhibition. Here we employed a rational engineering strategy to alleviate substrate inhibition by enhancing the dissociation efficiency of NAD⁺. Notably, the variant H184A exhibited a 1.53-fold increase in enzyme activity and a 58% improvement in the half-maximal inhibitory concentration for 2-oxobutyric acid, alleviating substrate inhibition.</p>\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Validation of a Three-Step Screening Strategy for Extracellular Salt-Tolerant Nucleases From Marine Bacteria","authors":"Aditya Achar,&nbsp;Subhojit Sen","doi":"10.1002/biot.70048","DOIUrl":"https://doi.org/10.1002/biot.70048","url":null,"abstract":"<p>This study reports the development of a 3-step strategy that is both cost-effective and quick to screen marine organisms and validate the presence of extracellular nucleases. The assay plates (M9 or Luria Broth [LB] media with 500 mM salt) were overlaid with a thin layer of top agar containing Toluidine Blue as the indicator and salmon sperm DNA as the substrate. Primary screening of halophiles was based on their zone of clearance. Secondary screening of the isolates involved assaying the supernatants using a well-diffusion assay. The isolates were further screened and validated by ammonium sulfate fractionation of the cell-free supernatants to enrich the secreted nuclease. The three-step method narrowed down nine potential isolates from ∼500 bacterial colonies, of which SH1 demonstrated nuclease activity, discernibly due to a secreted extracellular enzyme(s). Further characterization of this enriched nuclease(s) showed that it is likely made up of multiple peptides/subunits, acts as an endo- as well as exonuclease, degrades both DNA and RNA, is Mg⁺² dependent, has a wide range of salt tolerance from 80–1500 mM, is optimally active at 37°C, and is stable against reducing agents. This validates the screening strategy thus opening doors to further bioengineering of novel nucleases from other extremophiles.</p>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/biot.70048","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Replacement of Isoleucine and Leucine by their Keto Acids leads to increased formation of α-Hydroxy Acids in Chinese Hamster Ovary cells","authors":"Philipp Reifenberg,&nbsp;Lara Rosenberger,&nbsp;Maxime Le Mignon,&nbsp;Aline Zimmer","doi":"10.1002/biot.70041","DOIUrl":"https://doi.org/10.1002/biot.70041","url":null,"abstract":"<p>Keto isoleucine and keto leucine are bioavailable amino acid precursors in CHO cells producing biotherapeutics. Following their supplementation in a fed-batch culture with CHOK1 GS cells, quantitation of metabolites derived from the three branched-chain keto acids revealed a decrease of their decarboxylation products, but an accumulation of their α-hydroxy acids. The latter had minor effects on cell growth and productivity. L-lactate dehydrogenase was identified as the key enzyme forming valine's α-hydroxy acid.</p>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/biot.70041","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glutamine Attenuates Inflammation and Oxidative Stress in Ulcerative Colitis by Suppressing Wilms' Tumor 1 Associated Protein-Mediated N6-Methyladenosine Modification of Nuclear Receptor Coactivator 3","authors":"Liangxian Jiang,&nbsp;Shuai Liu,&nbsp;Feifei Ye,&nbsp;Xiaowei Yu","doi":"10.1111/cbdd.70110","DOIUrl":"https://doi.org/10.1111/cbdd.70110","url":null,"abstract":"<div>\u0000 \u0000 <p>Ulcerative colitis (UC) is a chronic inflammatory condition. Glutamine (Gln) has shown an improved effect on UC. However, its molecular determinants are incompletely understood. NCM460 cells were stimulated with lipopolysaccharide (LPS) to generate an in vitro UC cell model, and dextran sulfate sodium (DSS)-induced UC models were established in mice. Methylated RNA immunoprecipitation (MeRIP) and messenger RNA (mRNA) stability experiments were used to validate the influence of Wilms' tumor 1-associating protein (WTAP) on nuclear receptor coactivator-3 (NCOA3) mRNA. In LPS-exposed NCM460 cells, Gln promoted NCOA3 expression and reduced WTAP expression. Gln relieved LPS-triggered inflammation, oxidative stress, and apoptosis in NCM460 cells, which were abolished by NCOA3 downregulation or WTAP upregulation. Mechanistically, Gln suppressed WTAP-mediated m6A modification of NCOA3 mRNA. WTAP reduction attenuated LPS-evoked NCM460 cell phenotype alterations, which were reversed by NCOA3 downregulation. Furthermore, Gln reduced the DAI score and histopathological changes, increased colon length, and attenuated inflammation and oxidative stress in DSS-induced UC mice, which were abrogated by WTAP increase. We showed that the WTAP/NCOA3 axis underlies the protective effect of Gln on UC, providing a rationale for Gln as a promising anti-UC agent.</p>\u0000 </div>","PeriodicalId":143,"journal":{"name":"Chemical Biology & Drug Design","volume":"105 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RsWRKY49 promotes cold tolerance via activating the expression of RsCBF2 and RsNR2 in radish (Raphanus sativus L.)","authors":"Sen Chen,&nbsp;Nannan Liao,&nbsp;Hongyu Bi,&nbsp;Liang Xu,&nbsp;Yan Wang,&nbsp;Baozhen Mao,&nbsp;Xiaoli Zhang,&nbsp;Feng Cui,&nbsp;Yingbo Ma,&nbsp;Liwang Liu","doi":"10.1111/tpj.70256","DOIUrl":"https://doi.org/10.1111/tpj.70256","url":null,"abstract":"<div>\u0000 \u0000 <p>Cold stress adversely affects crop growth and development. WRKY transcription factors play the critical role in regulating abiotic stress response and balancing plant growth and cold stress defense. However, the roles of WRKY in the trade-off between root growth and cold stress response are still rarely known in radish. Herein, <i>RsWRKY49</i> was specifically expressed in the radish root and the expression was highly induced by low temperature in the root of cold-tolerant radish genotype ‘NAU-RG’ compared to the cold-sensitive radish genotype ‘NAU-XBC’. Overexpression of <i>RsWRKY49</i> in ‘NAU-XBC’ enhanced cold tolerance, while interference of its expression in ‘NAU-RG’ increased cold sensitivity. The increase in cell division activity and root meristem size was observed in the radish hairy root overexpressing <i>RsWRKY49</i> under both normal and low-temperature conditions, demonstrating its ability to regulate cold stress response and root growth. Natural variation in the <i>RsWRKY49</i> promoter affects the differences in its expression level in different cold-tolerant radish genotypes, thereby modulating cold tolerance. Comparative promoter analysis identified additional <i>cis</i>-acting regulatory elements (ten TATA boxes, two ABRE elements, and one DRE element) in the <i>RsWRKY49</i> promoter of ‘NAU-RG’, which showed enhanced promoter activity compared to that of ‘NAU-XBC’ under cold stress. In addition, RsWRKY49 could transactivate <i>RsCBF2</i> and <i>RsNR2</i> expression to regulate cold stress response. These results provide insights into the molecular mechanism underlying WRKY TFs balancing root growth and defense to cold stress in radish and would facilitate achieving genetic improvement of cold-tolerant cultivars in radish breeding programs.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soft Pneumatic Device Designed to Mimic the Periosteal Environment for Regulating the Fate of Mesenchymal Stem Cells (Adv. Healthcare Mater. 15/2025)","authors":"Gwang-Bum Im,&nbsp;Jae Gyeong Lee,&nbsp;Hosub Lim,&nbsp;Jae-Won Lee,&nbsp;Hyun Su Park,&nbsp;Yongju Kim,&nbsp;Nauman Asad,&nbsp;Hak-Rin Kim,&nbsp;Jeong Jae Wie,&nbsp;Suk Ho Bhang","doi":"10.1002/adhm.202570093","DOIUrl":"https://doi.org/10.1002/adhm.202570093","url":null,"abstract":"<p><b>Soft Pneumatic Tissue Mimicking Device</b></p><p>In article 2403229, Jeong Jae Wie, Suk Ho Bhan, and co-workers introduce a cutting-edge soft pneumatic device (SPD) that mimics the complex mechanical forces of the periosteum during movement, bridging biomechanics and stem cell biology. By integrating shear stress, strain, and fluid dynamics, SPD directs mesenchymal stem/progenitor cell fate—promoting osteogenic differentiation under mild forces while inducing controlled detachment under higher loads. Unlike conventional systems, SPD preserves the extracellular matrix, offering a transformative platform for studying mechanotransduction, bone regeneration, and cell therapy.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":"14 15","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adhm.202570093","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}