Wiley最新文献

{"title":"The Dual-Active-Site Catalysts Containing Atomically Dispersed Pr³⁺ with Ni/CeO₂ for CO₂ Hydrogenation to Methane.","authors":"Neha Choudhary, Navdeep Srivastava, Harshini V Annadata, Biplab Ghosh, Patrick Da Costa","doi":"10.1002/smll.202504707","DOIUrl":"https://doi.org/10.1002/smll.202504707","url":null,"abstract":"<p><p>In this study, uniformly dispersed Pr³⁺ as an isolated atom over Ni/CeO₂ catalyst (Ni-Pr/CeO₂) is designed to enhance catalytic activity for CO₂ methanation, achieving an impressive 87% conversion with ≈100% CH₄ selectivity at 300 °C temperature. In contrast, the traditional Ni/CeO₂ and NiPr/CeO₂-imp catalysts exhibit poor conversion and selectivity, highlighting the proof of concept on the advantage of atomic-scale dispersion. Structural analysis via PXRD, XAS, and XPS confirms the successful incorporation of Pr³⁺ into the CeO₂ lattice by creating defects. XPS and XAS studies further reveal a significant increase in oxygen vacancies, a key factor in enhancing catalytic performance at lower reaction temperatures. STEM-EDS analysis confirms the ultra-dispersion of Pr³⁺ (≈7 wt.%) over CeO₂, ensuring a highly active catalyst surface. H₂-TPR and CO₂-TPD results suggest that the Pr³⁺ doping enhances the catalytic activity by decreasing the reduction temperature and increasing basic sites. Additionally, long-term stability tests demonstrate no significant loss in activity over 40 h, confirming the catalyst's robustness and recyclability. This work provides critical insights into the structure-activity relationship of Pr³⁺-modified Ni/CeO₂ catalysts, emphasizing the role of oxygen vacancies in optimizing CO₂ hydrogenation efficiency.</p>","PeriodicalId":228,"journal":{"name":"Small","volume":" ","pages":"e2504707"},"PeriodicalIF":13.0,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral Combined Probiotics Clostridium butyricum and Akkermansia muciniphila Inhibits the Progression of 4T1 Breast Cancer by Activating Bcl-2/Bax Pathway","authors":"Xiaowei Li,&nbsp;Dengxiong Hua,&nbsp;Daoyan Wu,&nbsp;Wei Hong,&nbsp;Yingqian Kang,&nbsp;Lei Tang,&nbsp;Qin Yang,&nbsp;Xinxin Wang,&nbsp;Boyan Li,&nbsp;Renmin Li,&nbsp;Zhenghong Chen,&nbsp;Guzhen Cui","doi":"10.1002/cam4.70987","DOIUrl":"https://doi.org/10.1002/cam4.70987","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Breast cancer is the most common malignant tumor among women. Recent studies have found that gut probiotics and their metabolic products play a significant role in activating the immune system, reshaping the tumor microenvironment, and inhibiting cancer progression.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We established a 4T1 tumor-bearing mice model and analyzed the proportions of CD4⁺ T and CD8⁺ T cells in the spleen using flow cytometry and immunohistochemistry. The expression levels of TNF-α, IL-6, and IL-10 were measured by enzyme-linked immunosorbent assay. Hematoxylin–eosin staining was used to observe the tumor morphology. Selective protein blotting and quantitative real-time PCR were used to analyze the expression of Bax, Bcl-2, and Caspase-3. Cell proliferation was evaluated using the MTT assay, and apoptosis was detected by flow cytometry.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The results indicated that oral administration of CB and AKK possesses the capability to inhibit the progression of 4T1 breast cancer; however, the combined treatment with both strains (CB-AKK) exhibited significantly superior effects compared to each individual strain. Further mechanistic analysis revealed that the CB-AKK combination could activate the antitumor immunity in mice and reshape the tumor microenvironment. Additionally, it was found that the live bacteria and their metabolites derived from CB-AKK could inhibit cell proliferation and promote tumor apoptosis by activating the Bcl-2/Bax signaling pathway.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study is the first to demonstrate that orally administered live bacteria CB-AKK can inhibit the progression of 4T1 breast cancer, providing a promising new strategy for the development of innovative biotherapies for breast cancer.</p>\u0000 </section>\u0000 </div>","PeriodicalId":139,"journal":{"name":"Cancer Medicine","volume":"14 11","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cam4.70987","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144256028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current treatment guidelines and glycated haemoglobin goals for type 2 diabetes: Which patients are most likely to benefit from fixed-ratio basal insulin glucagon-like peptide-1 receptor agonist combinations?","authors":"Kevin Cowart, Nicholas W Carris","doi":"10.1111/dom.16502","DOIUrl":"https://doi.org/10.1111/dom.16502","url":null,"abstract":"<p><p>Basal insulin and glucagon-like peptide-1 receptor agonists (GLP-1 RAs) are available in two fixed-ratio combinations (FRCs) for the treatment of type 2 diabetes (T2D), insulin degludec with liraglutide and insulin glargine with lixisenatide. FRCs offer a convenient strategy to administer basal insulin and a GLP-1 RA, taking advantage of complementary mechanisms in a single injection to minimize treatment burden and improve glycemic control while mitigating weight gain. For most, a glycated haemoglobin (HbA1c) goal for patients with T2D using a FRC would be <7%, though it can be adjusted based on patient-specific factors. An HbA1c goal of <6.5% is less likely to be appropriate for patients requiring basal insulin, which is included in the FRCs. Ideal candidates for FRCs are those (1) taking oral antihyperglycemic medications only with an HbA1c <10% and <2% away from goal, (2) those on basal insulin and above HbA1c goal, (3) those unwilling or unable to initiate or manage multiple daily injections (i.e., basal-bolus insulin therapy) and (4) those tolerating a GLP-1 RA who require basal insulin to achieve glycemic goals.</p>","PeriodicalId":158,"journal":{"name":"Diabetes, Obesity & Metabolism","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DDM1 Controls Heritable Natural Epigenetic Variation in Arabidopsis.","authors":"Peng Zhang, Datong Xing, Muhammad Shahbaz Chishti, Linan Xie, Qingzhu Zhang","doi":"10.1111/mec.17828","DOIUrl":"https://doi.org/10.1111/mec.17828","url":null,"abstract":"<p><p>As a part of epigenetic modifications, DNA methylation involves the addition of a methyl group to cytosine. In plants, this process occurs in three sequence contexts (CG, CHG and CHH) through two pathways: de novo DNA methylation and DNA methylation maintenance. DNA methylation is highly conserved between ecotypes due to its heritability and role in genomic stability. However, numerous naturally occurring differentially methylated regions (NMRs) exist between ecotypes, which are also heritable and contribute to differential gene expression and phenotypic variation. Decreased DNA methylation observed in mutants of RdDM or DNA methylation maintenance pathways highlights the potential roles of these pathways in NMR formation and inheritance. Nevertheless, the complexity of plant DNA methylation across different contexts and pathways has made the contributions of these two pathways in NMR formation and inheritance remain unclear. To address this, we chose two Arabidopsis thaliana ecotypes, Col-0 (Col) and C24, and utilised mutants of pol4/5 and ddm1 in both ecotypes. By examining the changes in NMRs within these mutants, we aimed to elucidate the roles of DNA methylation pathways in NMR formation and inheritance. Our results revealed (1) NMRs can be categorised into three types, but classification is not solely based on methylation contexts; (2) compared to RdDM, the DNA methylation maintenance pathway plays a more dominant role in NMR formation and is less influenced by SNPs; (3) DNA methylation maintenance, rather than RdDM, controls NMR inheritance. Our findings highlight the role of the DNA methylation maintenance pathway in NMR formation and inheritance.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":" ","pages":"e17828"},"PeriodicalIF":4.5,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-Range Spin Transport in Chiral Gold.","authors":"Tapan Kumar Das, Offek Marelly, Shira Yochelis, Yossi Paltiel, Ron Naaman, Jonas Fransson","doi":"10.1002/adma.202506523","DOIUrl":"https://doi.org/10.1002/adma.202506523","url":null,"abstract":"<p><p>Any attempt to use spintronics-based logic elements will need to have spin interconnects to transfer information between its elements. Typically, the mean free path of an electron's spin in metals, at room temperature, is of the order of tens to hundreds of nanometers. Here chiral gold films are used to demonstrate that spin information can be transferred to distances of several microns at room temperature. The conduction of spins is accompanied by a Hall effect that exists without applying an external magnetic field. It is verified that the spin diffusion length is consistent with the frequency-dependent Hall effect which indicates a spin-effective lifetime in the order of nanoseconds. A theoretical model is presented that involves the anisotropic electronic polarizability of the system, its spin-orbit coupling, and spin exchange interactions.</p>","PeriodicalId":114,"journal":{"name":"Advanced Materials","volume":" ","pages":"e2506523"},"PeriodicalIF":27.4,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of DnaB-DnaG Interaction in M. tuberculosis using a SAXS-Based Dissociation Assay.","authors":"Barak Akabayov, Adi Dayan, Stefan Ilic","doi":"10.1002/cbic.202500289","DOIUrl":"https://doi.org/10.1002/cbic.202500289","url":null,"abstract":"<p><p>We studied the complex interactions between helicase and primase, two key components of the replisome involved in DNA replication in Mycobacterium tuberculosis. Utilizing purified, complementary domains of these proteins, we employed a surface plasmon resonance (SPR) analysis and a cross-linking assay to characterize their binding dynamics. The SPR analysis revealed a binding dissociation constant of 0.21 ± 0.08 µM, and the cross-linking assay suggested the possible formation of a heterodimer species. Importantly, we utilized a small-angle X-ray scattering (SAXS) dissociation assay to study the dynamic interactions between the proteins in solution. Our findings provide new opportunities for targeted therapeutic strategies aimed at DNA replication in M. tuberculosis by revealing the structural interplay between helicase and primase.</p>","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202500289"},"PeriodicalIF":2.6,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemoenzymatic Synthesis of 3-Halochromones via Oxidative α-Halogenation of Enaminones in TPGS-750-M Micelles.","authors":"Chisanu Krongyut, Jakkarin Limwongyut, Nittaya Wiriya, Anyanee Kamkaew, Ailada Jantasin, Rung-Yi Lai","doi":"10.1002/cbic.202500277","DOIUrl":"https://doi.org/10.1002/cbic.202500277","url":null,"abstract":"<p><p>Synthesis strategies of chromones have been widely investigated due to the abundance of chromone moiety in bioactive compounds and natural products. Of which, 3-halochromones are a versatile set of precursors to synthetically access valuable compounds with chromone frameworks. Generally, 3-halochromones were synthesized from o-hydroxy enaminones through oxidative α-halogenation, a process that often uses toxic and corrosive chemicals. Herein, we present an alternative strategy of oxidative α-halogenation catalyzed by vanadium-dependent chloroperoxidase from Curvularia inaequalis (CiVCPO) with H2O2/KX (X = Cl, Br, and I) in an aqueous medium. With a micellar system from a surfactant TPGS-750-M, substrate concentration can be increased to 50 mM without compromising the yield, thereby significantly reducing the use of organic solvents. Substrate scope investigation revealed that bromination and chlorination processes prefer electron-donating substituents although moderate electron-withdrawing groups were tolerated (20 examples). Additionally, iodination processes can be performed under the optimized condition. However, slow conversion indicated that further optimization is needed. We also found that iodination can occur without CiVCPO, albeit at a lower conversion. Further investigation suggested that such a conversion took place via I2 generated in situ. Overall, this chemoenzymatic method could offer an environmentally friendly approach to access a variety of 3-bromo or 3-chlorochromones.</p>","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202500277"},"PeriodicalIF":2.6,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Needle in a Haystack: A Droplet Digital Polymerase Chain Reaction Assay to Detect Rare Helminth Parasites Infecting Natural Host Populations.","authors":"Chloe A Fouilloux, Eric Neeno-Eckwall, Ipsita Srinivas, Jonathan S Compton, Josh Sampson, Jesse Weber, Cole Wolf, Amanda Hund, John Berini, Heather Alexander, Emma Choi, Daniel I Bolnick, Jessica L Hite","doi":"10.1111/1755-0998.14131","DOIUrl":"https://doi.org/10.1111/1755-0998.14131","url":null,"abstract":"<p><p>Helminths infect humans, livestock, and wildlife, yet remain understudied despite their significant impact on public health and agriculture. Because many of the most prevalent helminth-borne diseases are zoonotic, understanding helminth transmission among wildlife could improve predictions and management of infection risks across species. A key challenge to understanding helminth transmission dynamics in wildlife is accurately and quantitatively tracking parasite load across hosts and environments. Traditional methods, such as visual parasite identification from environmental samples or infected hosts, are time-consuming, while standard molecular techniques (e.g., PCR and qPCR) often lack the sensitivity to reliably detect lower parasite burdens. These limitations can underestimate the prevalence and severity of infection, hindering efforts to manage infectious diseases. Here, we developed a multiplexed droplet digital PCR (ddPCR) assay to quantify helminth loads in aquatic habitats using 18S rRNA target genes. Using Schistocephalus solidus and their copepod hosts as a case study, we demonstrate ddPCR's sensitivity and precision. The assay is highly reproducible, reliably detecting target genes at concentrations as low as 1 pg of DNA in lab standards and field samples (multi-species and eDNA). Thus, we provide a toolkit for quantifying parasite load in intermediate hosts and monitoring infection dynamics across spatio-temporal scales in multiple helminth systems of concern for public health, agriculture, and conservation biology.</p>","PeriodicalId":211,"journal":{"name":"Molecular Ecology Resources","volume":" ","pages":"e14131"},"PeriodicalIF":5.5,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photocrosslinkable Hydrogel Microparticle Bioink for Digital-Light-Processing 3D Bioprinting.","authors":"Shuiling Jin, Yanzhen Jing, Haowen Lu, Lufeng Shi, Mengying An, Xuesong Ye, Yifan Wang, Xiujun Cai, Shangjing Xin","doi":"10.1002/smtd.202500115","DOIUrl":"https://doi.org/10.1002/smtd.202500115","url":null,"abstract":"<p><p>Digital light processing (DLP) 3D bioprinting is a powerful technique for fabricating intricate tissue structures, yet it faces challenges related to limited bioink selection, difficulty in printing heterogeneous materials, and poor cell incorporation. In this study, photocrosslinkable hydrogel microparticle (HMP) bioinks tailored for DLP bioprinting are developed and optimized. Unlike traditional extrusion-based printing of HMPs that rely on jamming, the DLP approach eliminates this constraint, enabling improved printing resolution and cytocompatibility. It is demonstrated that the aqueous components, such as crosslinkers and photoinitiators, mixed in HMP bioinks ensure sufficient fluidity, supporting consistent DLP printing across a broad range of parameters. Small HMPs (28.2 ± 1.78 µm) provided better structural fidelity than large HMPs (75.0 ± 4.73 µm). Optimized crosslinking conditions and finely tuned UV exposure settings further enabled high-resolution printing, including the formation of serpentine inner channels. Importantly, cell-laden HMP bioinks maintained high viability, achieved uniform cell distribution within printed constructs, and supported their continued growth due to the void spaces between HMPs. The modular nature of HMPs also allowed straightforward multi-material printing. This work expands the application of HMP bioinks to DLP bioprinting, offering a versatile platform for biomanufacturing tissue constructs with microscopic compartmentalization for tissue engineering applications.</p>","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500115"},"PeriodicalIF":10.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"5-ALA Assisted Surgery of Human Glioblastoma Samples Reveals an Enrichment of T Cells Expressing PD-1 and CD103 in the Intermediate and Marginal Layers","authors":"Anna Vanni,&nbsp;Francesca Matani,&nbsp;Camilla Bonaudo,&nbsp;Alessio Mazzoni,&nbsp;Manuela Capone,&nbsp;Giulia Lamacchia,&nbsp;Lorenzo Salvati,&nbsp;Lucia Bartoli,&nbsp;Stefania Francalanci,&nbsp;Mirko Petti,&nbsp;Federico Capelli,&nbsp;Filippo Nozzoli,&nbsp;Lorenzo Cosmi,&nbsp;Francesco Liotta,&nbsp;Alessandro Della Puppa,&nbsp;Laura Maggi,&nbsp;Francesco Annunziato","doi":"10.1002/eji.202451681","DOIUrl":"https://doi.org/10.1002/eji.202451681","url":null,"abstract":"<p>Glioblastoma is the most common malignant brain tumor in adults, for which immunotherapy shows reduced efficacy. Current knowledge on immunotherapy failure is limited and detailed information about immune infiltrates in glioblastoma is urgently needed. We enrolled 34 glioblastoma patients collecting peripheral blood (PB), total tumor resection, or tumor from the necrotic area, the intermediate, and the marginal tissue through 5-aminolevulinic-acid (5-ALA) assisted surgery. T cells were evaluated for immune checkpoints and tissue residence memory (Trm) cell markers expression, and their cytokine production profile. Biological data were correlated with the patient's overall survival. Flow cytometry analysis showed a significantly higher frequency of T lymphocytes expressing PD-1, Trm markers in glioblastoma than in PB. In particular, we observed a preferential enrichment of CD8 cells expressing PD-1 and Trm markers in the intermediate and marginal tissue. T cells cytokine production resulted in increased glioblastoma compared with PB, in particular in PD-1+ cells and in the intermediate and marginal layers. These data suggest that CD103+ T-cell frequency in the core and TNF-a+CD8+ T cells in the intermediate layer influence the patient's survival. In conclusion, T cells obtained from different GBM layers showed different phenotypes and cytokines expression, suggesting new prognostic factors and supporting surgery particle strategy.</p>","PeriodicalId":165,"journal":{"name":"European Journal of Immunology","volume":"55 6","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/eji.202451681","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}