Wiley最新文献

{"title":"Localized Surface Plasmon Resonance-Enhanced SiC UV Photodetectors Based on Ordered Al/Al₂O₃ Core-Shell Nanoparticle Arrays.","authors":"Zhiyuan Zhang, Yinze Hu, Zhao Fu, Zihao Li, Jiadong Chen, Meng Yuan, Shaoxiong Wu, Rongdun Hong, Dingqu Lin, Xiaping Chen, Jiafa Cai, Zhengyun Wu, Yuning Zhang, Deyi Fu, Zhanwei Shen, Zhijie Wang, Feng Zhang, Rong Zhang","doi":"10.1002/smll.202502011","DOIUrl":"https://doi.org/10.1002/smll.202502011","url":null,"abstract":"<p><p>4H-SiC-based ultraviolet (UV) photodetectors (PDs) are urgently required for applications in flame detection and secure communication. However, these devices are hindered by their low quantum efficiency properties and sluggish response speed. Here, a substantial enhancement in UV detection is implemented by integrating periodic triangular Al/Al₂O₃ Core-Shell Nanoparticles (NPs) Arrays into 4H-SiC metal-semiconductor-metal (MSM) PDs. The detector exhibits an extremely low dark current (5.0 × 10^-14 A) and a peak responsivity of 2.14 A W^-1, corresponding to an external quantum efficiency of 984%. A high detectivity of 1.22 × 10¹⁴ Jones is achieved under illumination of 270 nm wavelength light at 30 V, while an ultra-high response speed is obtained with a rise time of 0.74 ns and a fall time of 1.47 ns. The improvement is attributed to the coupling between the lightning rod effect at the tips of the triangular NPs within the electrostatic field and localized surface plasmon resonance (LSPR), as well as the LSPR coupling effect between NPs, which enhances the electric field of the devices and triggers a localized avalanche effect. These results highlight the wide application and potential of NPs-enhanced 4H-SiC-based UV PDs in high-speed and high-precision detection.</p>","PeriodicalId":228,"journal":{"name":"Small","volume":" ","pages":"e2502011"},"PeriodicalIF":13.0,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of Boronic Acid-Functionalized Core-Shell Mesoporous Silica-TiO₂ Nanocomposite for Specific Enrichment of Glycopeptides From Biological Samples.","authors":"Mohamed Adly Gab-Allah, Maryam Adelipour, Hyojin Hwang, Reham M Marzouk, Heejin Lim, Jooyeon Oh, Ji-Hyun Cha, Jaebeom Lee, Jeongkwon Kim","doi":"10.1002/smtd.202500660","DOIUrl":"https://doi.org/10.1002/smtd.202500660","url":null,"abstract":"<p><p>Protein N-glycosylation plays a pivotal role in regulating biological processes and is closely associated with various disease pathologies. However, the identification of N-glycopeptides typically requires efficient enrichment and advanced mass spectrometric methods due to their low abundance and interference from non-glycosylated peptides. Herein, a boronic acid-functionalized core-shell mesoporous silica-TiO₂ nanocomposite is proposed for selective enrichment of N-glycopeptides from biological samples. The material holds attractive merits, including hydrophilic interaction from silica, high BET surface area (122.8 m² g^-1), large porosity (pore size: 8.5 nm), and boronic acid functionality, enabling efficient and specific glycopeptide capture. The enrichment performance using the synthesized material is demonstrated using tryptic digests of human immunoglobulin G (IgG), achieving high binding capacity (25 mg g^-1), good repeatability, and selectivity (1:100) toward glycopeptides. A considerable number of N-glycopeptides (n = 35) are successfully enriched from a 5 µg IgG digest, and the detection sensitivity is evaluated to be as low as 33.3 fmol µL^-1. Finally, the approach is validated using N-glycopeptides from human serum digest. From merely 2 µL of serum, 215 glycopeptides corresponding to 91 glycoproteins are enriched and identified by nano-LC-MS/MS, demonstrating the promising potential of the synthesized material in glycoproteomics research.</p>","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500660"},"PeriodicalIF":10.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The case for homebrew AI in diagnostic pathology.","authors":"Julien Calderaro, Helen Morement, Frédérique Penault-Llorca, Stephen Gilbert, Jakob Nikolas Kather","doi":"10.1002/path.6438","DOIUrl":"https://doi.org/10.1002/path.6438","url":null,"abstract":"<p><p>Artificial intelligence (AI) methods for digital pathology have tremendous potential to improve cancer diagnostics, biomarkers, and ultimately patient care. These AI methods, if marketed and sold, require authorisation or clearance as in vitro diagnostic (IVD) devices by regulatory bodies like the Food and Drug Administration (FDA) in the USA or Notified Bodies in the European Union (EU). Many AI tools for digital pathology are unlikely to be commercially viable and taken up by commercial entities ready to navigate these complex and costly processes. However, a longstanding quality framework already exists that allows for lab-developed tests, colloquially known as 'homebrew' tests, that are locally validated and performed under the responsibility and oversight of the pathologist. Here we argue for advancing homebrew AI systems within this existing framework to enhance patients' access to supportive digital diagnostic tools. We outline how homebrew AI models are currently permitted under regulatory provisions in the USA and the European Union, how a new US FDA rule may effectively regulate them out of existence, and propose steps to facilitate the safe and effective integration of homebrew AI models in pathology practice. © 2025 The Author(s). The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.</p>","PeriodicalId":232,"journal":{"name":"The Journal of Pathology","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developmental rewiring of the NGAL/CUC/KLU network associated with pleiotropic roles of NGAL genes","authors":"Antoine Nicolas,&nbsp;Panagiotis Papadopoulos,&nbsp;Mattéo Caroulle,&nbsp;Bernard Adroher,&nbsp;Liudmila Chelysheva,&nbsp;Magali Goussot,&nbsp;Anne-Sophie Sarthou,&nbsp;Nicolas Arnaud,&nbsp;Aude Maugarny,&nbsp;Patrick Laufs","doi":"10.1111/tpj.70321","DOIUrl":"https://doi.org/10.1111/tpj.70321","url":null,"abstract":"<p>Gene regulatory networks (GRNs) play prominent roles in regulating developmental processes, and their modulation across species is a major source for evolutionary innovation. However, it remains poorly understood how GRNs are rewired between different organs within a single species. This question is particularly relevant for pleiotropic genes, which may exhibit organ-specific GRN modulations potentially reflecting their diverse functions. To address this, we investigated the <i>NGATHA-like</i> (<i>NGAL</i>) genes as a model for pleiotropic genes that regulate growth or patterning in multiple Arabidopsis organs via two distinct pathways involving the <i>CUP-SHAPED COTYLEDON</i> (<i>CUC</i>) and <i>KLUH</i> (<i>KLU</i>) genes. By combining genetic analysis with gene expression characterization, we uncovered significant organ-specific rewiring of the <i>NGAL</i>/<i>CUC</i>/<i>KLU</i> regulatory module. For instance, the regulation of growth by <i>NGAL</i> genes occurs through the <i>KLU</i> pathway in petals, while both the <i>KLU</i> and <i>CUC</i> pathways function downstream of <i>NGAL</i> to regulate cauline leaf growth. Our findings highlight that changes in gene expression patterns, potentially arising from developmental constraints, play a pivotal role in the organ-specific modulation of gene regulatory modules. Furthermore, gene regulatory modules at the molecular and functional levels do not always align perfectly, potentially due to the influence of additional regulatory mechanisms. Altogether, our findings reveal significant modulation of the GRNs associated with pleiotropic genes. We propose that this flexibility in GRNs facilitates gene pleiotropy.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70321","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidermotropic Metastatic Melanoma Presenting as Eruptive Primary Melanomas","authors":"Jennifer Strong,&nbsp;Mitchell J. Winkie,&nbsp;Patrick Hallaert,&nbsp;Scott B. Whitecar,&nbsp;Elaine S. Keung,&nbsp;Daniel Neelon,&nbsp;Karen G. Zeman,&nbsp;Michele M. Gage,&nbsp;William C. Schaffenburg,&nbsp;Meagan M. Simpson,&nbsp;Isaac Brownell","doi":"10.1111/pcmr.70037","DOIUrl":"https://doi.org/10.1111/pcmr.70037","url":null,"abstract":"<div>\u0000 \u0000 <p>We report on the use of molecular profiling to diagnose epidermotropic metastatic melanoma (EMM) in a patient who presented with eruptive primary melanomas. On histopathology, the patient's metastatic lesions resembled superficial spreading melanomas and were indistinguishable from independent primary melanomas. The patient's presumed primary melanoma was a stage IIIB nodular melanoma. Despite treatment with adjuvant nivolumab, the patient continued to form new superficial spreading melanomas. Due to suspicion for EMM, commercial panel sequencing was performed on tissue from four tumors. Comparison of reported somatic variants revealed a mutational profile that was conserved across all four lesions, establishing a diagnosis of stage IV EMM. Considering the progressive disease on immunotherapy, treatment was transitioned to encorafenib plus binimetinib, resulting in regression of existing lesions and cessation of new skin lesion formation. Aside from micrometastatic sentinel lymph node deposits from the presumed primary melanoma, the patient had no evidence of non-cutaneous metastases. EMM should be considered as a diagnosis for multiple superficial spreading melanomas arising synchronously or in rapid succession. As EMM and primary melanomas are often histopathologically indistinguishable, next generation sequencing is a valuable tool to confirm clonality and provide a definitive diagnosis.</p>\u0000 </div>","PeriodicalId":219,"journal":{"name":"Pigment Cell & Melanoma Research","volume":"38 4","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Twenty Years of Innovation: SAINT Paving the Way for Nanotechnology and Breaking New Ground Through Convergence of Next-Generation Technologies (Adv. Mater. 26/2025)","authors":"Il Jeon,&nbsp;Pil Jin Yoo,&nbsp;Ji Beom Yoo,&nbsp;Sungjoo Lee","doi":"10.1002/adma.202570176","DOIUrl":"https://doi.org/10.1002/adma.202570176","url":null,"abstract":"<p>SAINT 20th Anniversary Special Section\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":114,"journal":{"name":"Advanced Materials","volume":"37 26","pages":""},"PeriodicalIF":27.4,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adma.202570176","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144550850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperglycemia Aggravates 6-Hydroxydopamine-Induced Neuronal Ferroptosis via SLC7A11-Dependent Pathway in Diabetic PD Rat Model","authors":"Ya Zhao,&nbsp;Dan Wang,&nbsp;Yanwei Wang,&nbsp;Dan Mu,&nbsp;Lang Qu,&nbsp;Rong Li","doi":"10.1111/cns.70487","DOIUrl":"https://doi.org/10.1111/cns.70487","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The epidemiological link between diabetes mellitus (DM) and Parkinson's disease (PD) is well-established, but the mechanistic basis remains unclear. Chronic hyperglycemia, a hallmark of DM, may exacerbate PD pathogenesis, though the underlying molecular pathways are poorly defined.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Using an integrative approach combining metabolomic profiling, proteomic analysis, and molecular characterization in vitro and in vivo models, we investigated the role of the cystine/glutamate antiporter system in glucose-induced neuronal vulnerability. SLC7A11 expression was genetically restored, and adeno-associated viral vectors delivered SLC7A11 to the nigrostriatal pathway in a streptozotocin-induced diabetic PD rat model to evaluate neuroprotection.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Chronic high glucose impaired SLC7A11 function, reducing cystine uptake and depleting intracellular glutathione in dopaminergic neurons, increasing susceptibility to 6-hydroxydopamine-induced ferroptosis. SLC7A11 restoration rescued neuronal viability, restored redox homeostasis, and attenuated motor deficits and dopaminergic neuron loss in the diabetic PD model. Mechanistically, SLC7A11 enhanced glutathione synthesis and suppressed ferroptosis signaling pathways.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Chronic hyperglycemia disrupts the cystine/SLC7A11/glutathione axis, accelerating neuronal degeneration and linking DM to PD susceptibility. SLC7A11 emerges as a potential therapeutic target to mitigate neurodegeneration in diabetic individuals at risk for PD.</p>\u0000 </section>\u0000 </div>","PeriodicalId":154,"journal":{"name":"CNS Neuroscience & Therapeutics","volume":"31 7","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cns.70487","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144550874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-Guided Functionalized Extracellular Vesicles for Visualized, Controlled, and Efficient Renal Delivery for Enhanced Kidney Repair (Adv. Healthcare Mater. 17/2025)","authors":"Yuhao Chen,&nbsp;Chunjia Sheng,&nbsp;Tuo Xiao,&nbsp;Bo Jiang,&nbsp;Xieguanxuan Xu,&nbsp;Shaoyuan Cui,&nbsp;Wanjun Shen,&nbsp;Xumin Zheng,&nbsp;Xinru Guo,&nbsp;Xiangmei Chen,&nbsp;Chuyue Zhang,&nbsp;Guangyan Cai","doi":"10.1002/adhm.202570100","DOIUrl":"https://doi.org/10.1002/adhm.202570100","url":null,"abstract":"<p><b>Ultrasound-Guided Functionalized Extracellular Vesicles</b></p><p>In article 2404719, Chuyue Zhang, Guangyan Cai, and co-workers develop ultrasound-guided extracellular vesicles for visualized and enhanced renal delivery. By enhancing F-actin restoration and facilitating uptake, these engineered carriers overcome cisplatin-induced dysfunction. The cover is inspired by Chinese tale of Nezha's Rebirth, symbolizing kidney regeneration as Nezha's renewal. The immortal's power, akin to “ultrasound exposure,” promotes efficient endocytosis to enable kidney repair.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":"14 17","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adhm.202570100","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipocalin-2 Restores Soluble Guanylyl Cyclase-Dependent Dilation of the Afferent Arteriole After Renal Transplantation or Ex Vivo Hypoxia/Reoxygenation in Mice","authors":"Liang Zhao,&nbsp;Minze Xu,&nbsp;Anna Maria Pfefferkorn,&nbsp;Cem Erdogan,&nbsp;Hubert Schwelberger,&nbsp;Pinchao Wang,&nbsp;Pratik Hemant Khedkar,&nbsp;Marc Eigen,&nbsp;Falk-Bach Lichtenberger,&nbsp;Rusan Catar,&nbsp;En Yin Lai,&nbsp;Felix Aigner,&nbsp;Pontus B. Persson,&nbsp;Igor Maximilian Sauer,&nbsp;Andreas Patzak,&nbsp;Muhammad Imtiaz Ashraf","doi":"10.1111/apha.70077","DOIUrl":"https://doi.org/10.1111/apha.70077","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>Dilatory microvascular function is impaired in ischemia/reperfusion injury in the kidney. Nitric oxide independent activators of soluble guanylyl cyclase (sGC) provide renal protection by dilating microvessels and preserving perfusion, but their efficacy declines after severe hypoxia. This study explores whether lipocalin-2 (Lcn2), a key iron-transporting protein, can restore the sGC-mediated dilation in mouse afferent arterioles (AAs) after hypoxia/reoxygenation (<i>H</i>/<i>R</i>) and kidney transplantation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Dilation of isolated, angiotensin II (Ang II) pre-constricted, AAs was induced by application of sGC activator cinaciguat after pre-constriction with Ang II following <i>H</i>/<i>R</i> (<i>H</i>: 30 min, <i>R</i>: 10 min ± holo-rLcn2, apo-rLcn2, deferoxamine) and syngeneic kidney transplantation (cold ischemia: 30 min or 5.5 h, reperfusion: 20 h ± holo-rLcn2) in C57BL/6 mice. To corroborate the dilatory function at the organ level, vascular relaxation was assessed using an isolated mouse kidney perfusion system.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Dilation of AAs was impaired following <i>H</i>/<i>R</i>. Pretreatment with holo-rLcn2 (iron-bound) preserved dilation, whereas apo-rLcn2 (iron-free) had no effect. The reversal of holo-rLcn2's effect by deferoxamine confirmed the role of iron. AAs from kidney transplants showed reduced dilation compared to sham-operated controls, with greater impairment following prolonged ischemia. Treatment with holo-rLcn2 significantly improved dilatory function after extended cold ischemia (5.5 h), restoring it to levels seen with shorter ischemia (30 min). Ex vivo perfusion of the isolated mouse kidney with holo-rLcn2 enhanced cinaciguat-induced vascular relaxation, confirming its beneficial effect at the organ level.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study identifies a novel role for holo-rLcn2 in preserving renal vascular function post-<i>H</i>/<i>R</i> and kidney transplantation, apparently by upholding iron levels in vascular cells.</p>\u0000 </section>\u0000 </div>","PeriodicalId":107,"journal":{"name":"Acta Physiologica","volume":"241 8","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apha.70077","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic Insights Into Serum-Free Medium Adaptation and Temperature Reduction in Chinese Hamster Ovary Cell Cultures","authors":"Benjamin F. Synoground,&nbsp;Yogender Gowtham,&nbsp;Timothy Lindquist,&nbsp;Junessa Pressley,&nbsp;Derrick C. Scott,&nbsp;Christopher S. Saski,&nbsp;Sarah W. Harcum","doi":"10.1002/biot.70055","DOIUrl":"https://doi.org/10.1002/biot.70055","url":null,"abstract":"<p>Chinese hamster ovary (CHO) cells are widely used in recombinant biopharmaceutical production; yet, yields remain low, leading to high market prices. Improving product yield and quality has heavily relied on empirical characterization with limited insight into internal molecular dynamics. RNA-seq offers a powerful alternative to understand intracellular responses to process changes through gene expression measurement. In this study, three RNA-seq datasets across three CHO cell lines and four industrially relevant treatments were integrated to characterize the global transcriptome changes, construct a weighted gene co-expression network, assess the impact on recombinant anti-interleukin 8 (anti-IL8) immunoglobulin heavy and light chain transcript abundance, and expression of glycosylation genes. Treatments included adaptation to serum-free medium, low temperature, low pH, and low glucose concentration in the medium. The findings suggest upregulation of cholesterol biosynthesis is critical for serum-free medium adaptation, and the rate-limiting enzymes in the sterol regulatory element-binding protein pathway (<i>Insig1</i> and <i>Srebf2</i>) could be targeted to accelerate adaptation. Temperature-induced cell cycle suppression was likely mediated by p53 activation, consistent with previous reports, with the p53-targets, <i>Zmat3</i> and <i>Btg2</i>, identified as key hub genes. Conversely, glucose and pH were observed to have negligible impacts on the transcriptome. This study uniquely identifies novel genes mediating temperature-induced cell cycle arrest, distinct glycosylation-related gene responses impacting product quality, and new stable housekeeping genes for accurate gene expression normalization in CHO cells.</p>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 7","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/biot.70055","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}