Wiley最新文献

{"title":"Environmental DNA Comparative Phylogeography: Simultaneous Estimation of Population Structures Within a Species-Rich Group of Freshwater Gobies.","authors":"Satsuki Tsuji, Shota Kunimatsu, Katsutoshi Watanabe","doi":"10.1111/mec.70059","DOIUrl":"https://doi.org/10.1111/mec.70059","url":null,"abstract":"<p><p>Comparative phylogeography provides crucial insights into evolutionary processes shaping biodiversity patterns by analysing spatial genetic variations across multiple species. However, conventional capture-based methods are often labour-intensive, particularly for multi-species analyses. Environmental DNA (eDNA) analysis has significant advantages in comparative phylogeography, including simplified field surveys requiring only water collection and the potential to simultaneously analyse multiple species from a single sample. To further expand the eDNA application and demonstrate its utility in comparative phylogeographic studies, this study employed eDNA analysis to simultaneously analyse the phylogeographic patterns in a species-rich freshwater goby group (Rhinogobius) in the Japanese Archipelago. DNA amplification was performed on eDNA samples collected from 573 sites across the archipelago using newly designed group-specific primers targeting the mitochondrial cytochrome b region of Rhinogobius. High-throughput sequencing detected haplotypes of all nine known species (or species groups) occurring in this region, followed by phylogenetic and network analyses. The eDNA analysis successfully revealed the genetic population structures across multiple species. A landlocked species, R. flumineus, exhibited fine-scale population differentiation shaped by geomorphological barriers, while amphidromous species showed broader genetic patterns likely influenced by ocean currents and their ecological traits. The phylogenetic and phylogeographic patterns reconstructed by the eDNA analysis were almost completely concordant with previously identified patterns of limited groups based on conventional methods, demonstrating the reliability of eDNA-based comparative phylogeography. This study highlights the potential of eDNA to complement and partially replace conventional methods, facilitating large-scale comparative phylogeographic research to gain new insights into spatial patterns and evolutionary processes of biodiversity.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":" ","pages":"e70059"},"PeriodicalIF":3.9,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VoronaGasyCodes: A Public Database of Mitochondrial Barcodes for Malagasy Birds.","authors":"Sushma Reddy, Kristen Wacker, Mai Fahmy, Evon Hekkala, John M Bates, Steven M Goodman, Shannon J Hackett, Marie J Raherilalao, J Dylan Maddox","doi":"10.1111/1755-0998.70027","DOIUrl":"https://doi.org/10.1111/1755-0998.70027","url":null,"abstract":"<p><p>Molecular tools are increasingly being used to survey the presence of biodiversity and their interactions within ecosystems. Indirect methods, like environmental DNA (eDNA) and invertebrate-derived DNA (iDNA), are dependent on sequence databases with accurate and sufficient taxonomic representation. These methods are increasingly being used in regions and habitats where direct detection or observations can be difficult for a variety of reasons. Madagascar is a biodiversity hotspot with a high proportion of endemic species, many of which are threatened or endangered. Here we describe a new resource, VoronaGasyCodes, a curated database of newly published genetic sequences from Malagasy birds. Our database is currently populated with six mitochondrial genes or DNA barcodes for 142 species including 70% of the birds endemic to the island and will be periodically updated as new data become available. We demonstrate the utility of our database with an iDNA study of leech blood meals where we successfully identified 77% of the hosts to species. These types of resources for characterising biodiversity are critical for insights into species distribution, discovery of new taxa, novel ecological connections and advancing conservation and restoration measures.</p>","PeriodicalId":211,"journal":{"name":"Molecular Ecology Resources","volume":" ","pages":"e70027"},"PeriodicalIF":5.5,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the Role and Mechanism of Decidual Monocyte-Derived Macrophage Infiltration in Obstetric Antiphospholipid Syndrome at Single-Cell Resolution.","authors":"Rui Gao, Pingying Qing, Hanxiao Chen, Zhengyan Hu, Qiaoran Yang, Chenyang Lu, Huimin Liu, Rujun Zeng, Yuanting Tang, Fan Yu, Jinbiao Han, Xin Liao, Xun Zeng, Lang Qin","doi":"10.1002/advs.202503480","DOIUrl":"https://doi.org/10.1002/advs.202503480","url":null,"abstract":"<p><p>Obstetric antiphospholipid syndrome (OAPS) is an autoimmune disorder characterized by pathologic pregnancies and the presence of antiphospholipid antibodies (aPLs). Despite significant infiltration of decidual macrophages observed in OAPS patients, the underlying connections between decidual and peripheral immune cells remain unclear. In this study, an integrated single-cell atlas is constructed of the decidua and peripheral blood mononuclear cells (PBMCs) from OAPS patients and HCs. Using this atlas, substantial disparities are identified in immune cells between the decidua and PBMCs. The functional changes in immune cells of OAPS patients are also different in decidua and PBMCs. Moreover, increased infiltration of monocyte-derived macrophages (MDMs) into the decidua is found to contribute to inflammation and trophoblast dysfunction in OAPS. The role of CCL2 is further discovered in recruiting MDMs, driven by excess CCL2 secreted from decidual macrophages stimulated by the aPLs and β₂-glycoprotein I complex via the TLR4-NF-κB pathway. Decidual vascular endothelial cells express higher levels of ACKR1, which aggregates CCL2 on their surface. Targeting CCR2 and TLR4 improved pregnancy outcomes in OAPS mouse models induced by aPLs, suggesting that these pathways may serve as potential therapeutic targets for OAPS. This study provides new insights into the pathogenesis of OAPS, particularly regarding decidual MDMs infiltration.</p>","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e03480"},"PeriodicalIF":14.1,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving Tympanoplasty: Transverse External Auditory Canal Flap Advancement.","authors":"Xiaochen Gao, Wenwen Qi, Fangyu Yuan, Chengcheng Liu, Hui Li, Yuhan Dong, Xue Cao, Chengzhilin Li, Ming Xia, Fengyang Xie","doi":"10.1002/lio2.70222","DOIUrl":"https://doi.org/10.1002/lio2.70222","url":null,"abstract":"<p><strong>Objectives: </strong>We conducted this study to investigate the clinical outcomes of a new self-created technique for improving tympanoplasty. This technique, called the external auditory canal flap advancement method, aims to treat large tympanic membrane perforations, particularly those without residual edges at the anterior margin.</p><p><strong>Methods: </strong>We selected 30 patients (50 ears) with large tympanic membrane perforations located in the anterior part of the tympanic membrane without residual edges. We grouped these patients based on the surgical methods used. Twenty-six patients in the experimental group were treated with the new method of endoscopic transverse external auditory canal skin flap repair. The control group, consisting of 24 patients, received conventional endoscopic tympanoplasty. We assessed the patients for hearing, postoperative pain, and tympanic membrane healing both before and after the surgery.</p><p><strong>Results: </strong>Our new method significantly improves the success rate of tympanic membrane repair and hearing levels. The healing rate of tympanic membrane repair in the experimental group was 96.15% (25/26). Patient discharge time, postoperative pain, or recovery time was not affected.</p><p><strong>Conclusions: </strong>Endoscopic transverse external auditory canal flap nudge repair, as a complement to conventional otoscopic tympanoplasty, should be promoted in clinical practice.</p>","PeriodicalId":48529,"journal":{"name":"Laryngoscope Investigative Otolaryngology","volume":"10 4","pages":"e70222"},"PeriodicalIF":1.7,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12329914/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144800664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of the addition of antithymocyte globulin to post-transplantation cyclophosphamide in haploidentical transplantation with peripheral blood compared to post-transplantation cyclophosphamide alone: A retrospective study on behalf of the Cellular Therapy and Immunobiology Working Party of the European Society for Blood and Marrow Transplantation.","authors":"Antoine Capes, Jarl E Mooyaart, Didier Blaise, Stefania Bramanti, Mi Kwon, Mohamad Mohty, Patrice Chevallier, Jan Vydra, Péter Reményi, Edouard Forcade, Lucía López Corral, Maija Itälä-Remes, Ali Bazarbachi, Enrico Derenzini, Jorinde D Hoogenboom, Jürgen Kuball, Giorgia Battipaglia, Florent Malard, Annalisa Ruggeri","doi":"10.1111/bjh.70050","DOIUrl":"https://doi.org/10.1111/bjh.70050","url":null,"abstract":"<p><p>In the setting of haploidentical haematopoietic cell transplantation (HCT), post-transplant cyclophosphamide (PTCy) has dramatically reduced the incidence of graft-versus-host disease (GVHD) and non-relapse mortality. To further reduce GVHD incidence, the addition of antithymocyte globulin (ATG) to PTCy was evaluated in retrospective and non-comparative prospective studies showing promising results. We conducted a large retrospective analysis of the European Society for Blood and Marrow Transplantation (EBMT) registry to evaluate this approach. We analysed haploHCT with peripheral blood stem cells performed for haematological malignancies between 2014 and 2021. GVHD prophylaxis included either PTCy alone or PTCy+ATG. Four thousand five hundred and nineteen patients were analysed in the PTCy only group versus 675 with PTCy+ATG. Median follow-up was 29.80 months. In univariate analysis, 2-year GVHD-free, relapse-free survival (GRFS), relapse-free survival (RFS), overall survival (OS), cumulative incidence of relapse, non-relapse mortality (NRM) and chronic GvHD (cGVHD) were, respectively: 40.5% versus 37.5% (p = 0.098), 50.9% versus. 45.8% (p = 0.015), 56.9% versus 52.5% (p = 0.01), 24.2% versus 28.1% (p = 0.032), 25% versus 26.1% (p = 0.49) and 28.4% versus 18.5% (p < 0.001). aGVHD did not differ. After multivariable adjustment, OS and RFS were lower in the PTCy+ATG group: HR = 1.18 (p = 0.037) and HR = 1.18 (p = 0.027) and patients receiving PTCy+ATG had less cGVHD: HR = 0.68 (p = 0.004). In that retrospective analysis, the addition of ATG to PTCy for GVHD prophylaxis in haploHCT was associated with a reduction of cGVHD but also a worse OS and RFS.</p>","PeriodicalId":135,"journal":{"name":"British Journal of Haematology","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ion-Pairing Assemblies of Polarized Charged Porphyrin.","authors":"Ryo Kitayama, Yohei Haketa, Yuto Maruyama, Masaki Fujita, Hiroki Tanaka, Hiroki Horita, Hiromitsu Maeda","doi":"10.1002/chem.202502225","DOIUrl":"https://doi.org/10.1002/chem.202502225","url":null,"abstract":"<p><p>A porphyrin Au^III complex comprising 5,10-penatafluorophenyl units was synthesized as a dipolar π-electronic cation. The π-electronic cation was combined with anions, including a bulky borate anion and planar π-electronic anions. The dipolar π-electronic cation formed crystal-state ion-pairing assemblies based on antiparallel stacking structures, which were stabilized by interionic dipole-dipole interactions.</p>","PeriodicalId":144,"journal":{"name":"Chemistry - A European Journal","volume":" ","pages":"e02225"},"PeriodicalIF":3.7,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Opinion: Why Sex-Based Genomic Differentiation Should Not Be Overlooked in Population Genetics.","authors":"Yu-Chi Chen, Nikolas Vellnow, Justin J S Wilcox, Sahar Javaheri Tehrani, Toni I Gossmann","doi":"10.1111/mec.70061","DOIUrl":"https://doi.org/10.1111/mec.70061","url":null,"abstract":"<p><p>Sex-specific genomic differentiation is a crucial yet frequently overlooked factor in population genetics. In this opinion piece, we leverage the substantial genomic resources available for the great tit (Parus major), including population-scale data sets from many European populations, to investigate genomic differentiation between males and females. Unlike in some other species, where high-quality genome assemblies exist but broad population sampling is lacking, the great tit offers a unique opportunity to study sex-based differentiation at both the genomic and population level. We identify significant differentiation at an autosomal locus on chromosome 5, which we hypothesise originates from sex-linked variation present on the sex chromosomes (Z and potentially W). By referencing genomic data from other songbirds with well-assembled sex chromosomes, we illustrate how autosomal loci may exhibit high sequence similarity to sex-linked regions. Our analyses demonstrate that uneven sex ratios in sampled populations can substantially bias differentiation metrics (e.g., F_ST), potentially resulting in false-positive interpretations of adaptive differentiation. To mitigate such issues, we stress the importance of sex-aware study designs, including balanced sex sampling and explicitly incorporating sex as a covariate. Furthermore, while optimal study designs would include high-quality reference genomes from both sexes, we recommend, as a pragmatic and cost-effective alternative for labs with limited resources, generating a reference genome from the heterogametic sex (females in birds) to ensure both sex chromosomes are represented in mapping and analysis. Finally, we emphasise the need for rigorous validation of candidate loci to ensure accurate and biologically meaningful outcomes in evolutionary genomic studies.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":" ","pages":"e70061"},"PeriodicalIF":3.9,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Top-Down Fabricated Wood-Derived Pressure and Strain Sensors: A Review.","authors":"Yi Ren, Fuyao Liu, Yanan Zhong, Shengbo Ge, Zhipeng Shi, Siling Lin, Hongli Song, Yingying Zhang, Wei Fan","doi":"10.1002/advs.202507712","DOIUrl":"https://doi.org/10.1002/advs.202507712","url":null,"abstract":"<p><p>As the most abundant and renewable natural resource on earth, wood has been widely used in daily life and industry since ancient times owing to its low cost, facile processability, and environmental sustainability. In recent years, advanced wood-based materials have rapidly emerged/developed, among which wood-derived pressure/strain sensors have attracted attention. To date, most reviews on wood-based sensors have focused on bottom-up strategies, while relatively few have explored the top-down approach. However, the top-down strategies offer notable advantages in terms of process efficiency and reduced energy consumption. This review summarizes recent advances in top-down fabricated wood-derived pressure/strain sensors (TWPSS), including raw material selection, the influence of wood composition and microstructure on sensor performance, and top-down pretreatment methods. Furthermore, the characteristics, sensing mechanisms, and practical applications of TWPSS are analyzed. Finally, current challenges and future research directions are proposed. This review aims to provide insights and serve as a reference for further development of TWPSS.</p>","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e07712"},"PeriodicalIF":14.1,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How to Quickly Diagnose Catatonia, and a Farewell Salute to Max Fink, MD.","authors":"Dirk Dhossche, Lee Elizabeth Wachtel","doi":"10.1111/acps.70024","DOIUrl":"https://doi.org/10.1111/acps.70024","url":null,"abstract":"","PeriodicalId":108,"journal":{"name":"Acta Psychiatrica Scandinavica","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comment on \"Biology and Management of Male-Bodied Athletes in Elite Female Sports\" by Handelsman and Bermon.","authors":"Gregory A Brown, Brandon Shaw, Ina Shaw, Michael J Joyner, Sandra K Hunter, Jonathon W Senefeld, Ross Tucker, Emma Hilton, Tommy R Lundberg, Chad Carlson, Christopher Kirk","doi":"10.1002/dta.3916","DOIUrl":"https://doi.org/10.1002/dta.3916","url":null,"abstract":"","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}