Matthew C. Yates, Taylor M. Wilcox, Shannon Kay, Pedro Peres-Neto, Daniel D. Heath
{"title":"A Framework to Unify the Relationship Between Numerical Abundance, Biomass, and Environmental DNA","authors":"Matthew C. Yates, Taylor M. Wilcox, Shannon Kay, Pedro Peres-Neto, Daniel D. Heath","doi":"10.1002/edn3.70073","DOIUrl":"https://doi.org/10.1002/edn3.70073","url":null,"abstract":"<p>Does environmental DNA (eDNA) concentration correlate with numerical abundance (<i>N</i>) or biomass in aquatic organisms? We hypothesize that eDNA can be adjusted to simultaneously reflect both. Building on frameworks developed from the Metabolic Theory of Ecology, we derive two equations to adjust eDNA data to simultaneously reflect both <i>N</i> and biomass using population size structure data and allometric scaling coefficients. We also demonstrate that these equations share model parameters, necessitating the joint estimation of regressions between adjusted eDNA, <i>N</i>, and biomass. Furthermore, our framework can be extended to model how other variables (temperature, taxa, diet, trophic level, etc.) might impact relationships between eDNA, <i>N</i>, and biomass in natural ecosystems. We applied our framework to data from two previously published studies correlating eDNA to Brook Trout (<i>Salvelinus fontinalis</i>) <i>N</i> and biomass. In both case studies, point estimates of the scaling coefficient (<i>b</i>) reflected allometric processes (<i>b</i> = 0.51 and 0.37 for Case Study 1 and 2, respectively), with credible intervals indicating that b likely differed from zero (i.e., eDNA scales with <i>N</i>) and one (i.e., eDNA scales with biomass). Directly estimating the value of b improved estimates of <i>N</i> and biomass relative to assuming b equals 0, which particularly affected the capacity to estimate biomass. However, models assuming eDNA production scaled with biomass (i.e., <i>b</i> = 1) were largely similar to estimating <i>b</i>, implying that assuming eDNA scales linearly with biomass might be a sufficient approximation for some systems. Nevertheless, the framework demonstrates that correlating eDNA directly with either <i>N</i> or biomass (as is commonly done in many studies) inherently necessitates an adjustment to infer the other metric if populations exhibit size structure variation. Collectively, we demonstrate that quantitative eDNA data is unlikely to correspond exactly to either population <i>N</i> or biomass but can be adjusted to simultaneously reflect both.</p>","PeriodicalId":52828,"journal":{"name":"Environmental DNA","volume":"7 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/edn3.70073","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Derry Permana Yusuf, Josaphat Tetuko Sri Sumantyo, Yuki Yoshimoto, Steven Gao, Koichi Ito
{"title":"Dual-Band Circularly Polarised Asymmetric Linear-Tapered Horn Antenna for Feeder of Synthetic Aperture Radar Onboard Microsatellite","authors":"Derry Permana Yusuf, Josaphat Tetuko Sri Sumantyo, Yuki Yoshimoto, Steven Gao, Koichi Ito","doi":"10.1049/mia2.70006","DOIUrl":"https://doi.org/10.1049/mia2.70006","url":null,"abstract":"<p>This paper presents a novel dual-band waveguide-fed circularly polarised (CP) horn antenna for the feeder of synthetic aperture radar (SAR) onboard microsatellite. The proposed horn antenna consists of an asymmetric linearly tapered slot and a waveguide feed. The C-/X-band left-hand circular polarisation (LHCP) design was simulated, and the 3D-printed prototype was fabricated to validate the simulation and measured in an anechoic chamber. The measured circularly polarised (CP) antenna can achieve dual-band operations with a 3 dB axial ratio bandwidth of 40% (5–6.5 GHz; 8.5–10 GHz), VSWR less than 1.4 in the operating band and a peak gain of 13.6 dBic (5.3 GHz) and 18.5 dBic (9.4 GHz). The measured half-power beamwidth (HPBW) at C-band and X-band frequencies in the xz-plane and yz-plane are achieved at 26°/37° and 18°/23°, respectively. The proposed antenna offers several advantages over current CP antenna designs, including simpler design, low-cost manufacturing, better impedance matching, wider AR bandwidth and lower sidelobe levels.</p>","PeriodicalId":13374,"journal":{"name":"Iet Microwaves Antennas & Propagation","volume":"19 1","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/mia2.70006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"计算机科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Luis Andrés Yarzábal Rodríguez, Ramón Alberto Batista-García
{"title":"When Science Meets Creativity: Elevating Microbiology Education With Art—Two Personal Experiences","authors":"Luis Andrés Yarzábal Rodríguez, Ramón Alberto Batista-García","doi":"10.1111/1751-7915.70099","DOIUrl":"https://doi.org/10.1111/1751-7915.70099","url":null,"abstract":"<p>Science communicators are more critical than ever in a time when misinformation and hoaxes dominate social media, especially during global emergencies like the COVID-19 outbreak. Researchers are good at communicating with peers but often struggle to explain complex ideas to the public. As shown previously, expanding outreach by combining science and art is not only possible, but effective. In the case of microbiology, the synergy between these two apparently divorced areas can help educating our communities and raising microbiological awareness, especially among students. Since Alexander Fleming's pioneering attempts, microorganisms have fascinated artists. Art may help us demystifying microbes and making them more approachable, improving public involvement with science. This article presents two personal experiences using art to teach and communicate scientific ideas (mainly from the microbiology field) to our fellow citizens. Above all, we emphasise the importance of contributing to the scientific literacy of our societies at all ages—including underrepresented and disadvantaged groups—by exploring novel ways to address this gap. By doing so, we align with the concepts and aims of the International Microbiology Literacy Initiative (IMiLI).</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70099","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correction to EuPPollNet: A European Database of Plant-Pollinator Networks","authors":"","doi":"10.1111/geb.70014","DOIUrl":"https://doi.org/10.1111/geb.70014","url":null,"abstract":"<p>\u0000 <span>Lanuza, J.B.</span>, <span>T.M. Knight</span>, <span>N. Montes-Perez</span>, et al. <span>2025</span>. “ <span>EuPPollNet: A European Database of Plant-Pollinator Networks</span>.” <i>Global Ecology and Biogeography</i> <span>34</span>: e70000. https://doi.org/10.1111/geb.70000\u0000 </p><p>In the originally published article, funding information for some authors was missing. The missing information is included below.</p><p>JBL was funded by the Federal State of Saxony-Anhalt (MLU-BioDivFund). RT was supported by the Czech Science Foundation (Project No. 21-24186M). NDM, NH, YP and FM were financially supported by the ANR ARSENIC project (grant no. 14-CE02-0012), the ANR NGB project (grant no. 17-CE32- 011), the Region Nord-Pas-de-Calais, the CNRS, the French Ministère de l'Enseignement Supérieur et de la Recherche, the Hauts-de-France Region and the European Regional Funds.</p><p>We apologize for this error.</p>","PeriodicalId":176,"journal":{"name":"Global Ecology and Biogeography","volume":"34 3","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/geb.70014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Filip Hromčík, Adéla Halusková, Lydie Izakovičová Hollá
{"title":"Lateral Approach for Regenerative Treatment of Intrabony Defects Associated With an Edentulous Alveolar Ridge: A Prospective Case Series","authors":"Filip Hromčík, Adéla Halusková, Lydie Izakovičová Hollá","doi":"10.1002/cre2.70094","DOIUrl":"https://doi.org/10.1002/cre2.70094","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This case series evaluated the clinical efficacy of the novel “lateral approach” combined with an enamel matrix derivative (EMD) and bone grafting in the regenerative surgical treatment of intrabony defects associated with an edentulous ridge.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>The innovative flap, called the “lateral approach,” is explicitly designed for regeneration of unchallenged isolated intrabony defects associated with <i>edentulous</i> alveolar ridges. The flap is defined by a curved vertical incision on the buccal side opposite the treated defect and a sulcular incision on the buccal and defect-associated sides, promoting uneventful healing and regeneration while minimizing complications.</p>\u0000 \u0000 <p>Seven intrabony defects (one per patient) distal to the lower second molar were treated using the “lateral approach” combined with EMD and grafting with deproteinized bovine bone mineral. The primary outcome was clinical attachment level (CAL) change. As additional parameters, pocket probing depth (PPD) reduction and complication rate were analyzed. All the outcomes were assessed 6 months post-surgery and compared with the baseline values.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Primary wound healing occurred in 100% of cases, and no complications were reported. At the 6-month re-evaluation, the initial median CAL of 6 mm (interquartile range 5–8 mm) was reduced to 3 mm (3–5 mm). The corresponding median PPD was reduced from 6 mm (IQR 6–8 mm) to 4 mm (IQR 3–5 mm). These differences were statistically significant (<i>p</i> < 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The “lateral approach” is a technique for the surgical treatment of intrabony defects associated with the edentulous ridge. Within the limitations of the study, this method seems to be suitable for distal intrabony defects in the lower second molars, which frequently develop after third molar extraction.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70094","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of the Ochratoxin A Reduction and Physicochemical Properties of Green Coffee Beans Following Atmospheric Nonthermal Plasma and Plasma-Activated Water Treatments","authors":"Ashrie Yacab, Chia-Min Lin, Pei-Shan Chih, Trias Mahmudiono, Chih-Yao Hou, Hsiu-Ling Chen","doi":"10.1002/efd2.70040","DOIUrl":"https://doi.org/10.1002/efd2.70040","url":null,"abstract":"<p>Nonthermal plasma (NTP) is a novel nonthermal technology with many potential applications in the food industry because of its promising effects on food decontamination, including mycotoxin decontamination. In this study, atmospheric NTP and plasma-activated water (PAW) were used to evaluate ochratoxin A (OTA) reduction and the quality of green coffee beans. Artificially contaminated green coffee beans were subjected to atmospheric NTP, PAW, atmospheric NTP-PAW, and PAW-atmospheric NTP treatments for 2 min, and OTA was then analyzed using liquid chromatography with tandem mass spectrometry before and after each treatment. NTP-based treatments significantly reduced OTA concentration in green coffee beans by 37.66%–51.80%. The maximum reduction efficiency was found in PAW-atmospheric NTP treatment. Furthermore, no negative effects were observed in terms of color, caffeine content, total phenolic content, total flavonoid content, and DPPH scavenging activity, except for atmospheric NTP treatment, which exhibited distinct color changes. In addition, a minimal decrease in the aroma of green coffee beans was observed following atmospheric NTP and PAW treatments, whereas a minimal increase was observed when both treatments were combined. NTP-based treatments provide new approaches for mitigating mycotoxin in the food industry as well as important implications for the use of atmospheric NTP and PAW in the coffee industry.</p>","PeriodicalId":11436,"journal":{"name":"eFood","volume":"6 2","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ning Chen, Guo-yun Zhang, Ya-ting Song, Yang Yang, Jian-guo Zhang, Cai-yun He
{"title":"A chromosome-scale genome of Hippophae neurocarpa provides new insights into serotonin biosynthesis and chlorophyll-derived brown fruit coloration","authors":"Ning Chen, Guo-yun Zhang, Ya-ting Song, Yang Yang, Jian-guo Zhang, Cai-yun He","doi":"10.1111/tpj.70031","DOIUrl":"https://doi.org/10.1111/tpj.70031","url":null,"abstract":"<div>\u0000 \u0000 <p>Sea buckthorn (<i>Hippophae neurocarpa</i>), a plant with both medicinal and edible properties, exhibits high content of serotonin with a unique brown color. Here, we assemble the 1002.54 Mb genome sequence of <i>H. neurocarpa</i> and find that it has evolved from two sequential polyploidizations with four sub-genomes. Based on <i>in vitro</i> enzyme activity and transient overexpression experiments, we confirm that the expression of both <i>HnT5H1</i> and <i>HnT5H2</i> genes from tandem duplication contributes to high accumulation of serotonin in sea buckthorn. Furthermore, we verify that the <i>HnGLK1</i> gene plays a crucial role in continuous chlorophyll accumulation, driving the brown color formation of <i>H. neurocarpa</i> fruit. Collectively, the high-quality genome of <i>H. neurocarpa</i> offers valuable insights into the mechanisms underlying serotonin biosynthesis and abnormal coloration and serves as a valuable resource for further functional genomic studies and molecular breeding efforts in sea buckthorn.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Transcriptional Regulator DhyR Positively Modulates Daptomycin Biosynthesis in Streptomyces roseosporus","authors":"Fuqiang He, Xinpeng Liu, Haiyi Wang, Xu Li, Yun Wu, Dan Zhang, Shufang Liang","doi":"10.1111/1751-7915.70110","DOIUrl":"https://doi.org/10.1111/1751-7915.70110","url":null,"abstract":"<p>Daptomycin (DAP) is a cyclic lipopeptide antibiotic produced by <i>Streptomyces roseosporus</i>, and its biosynthesis is precisely regulated by a complex regulatory network. Although the biosynthetic pathway of DAP has been elucidated, the regulatory mechanism governing its biosynthesis at the transcriptional level is not yet fully understood. In the present study, a new transcriptional regulator, DhyR, was identified. A deletion mutant of <i>dhyR</i> was constructed using the CRISPR-Cas9 tool to elucidate the biological role of DhyR thanks to functional and transcriptomic analyses. The results demonstrated that DhyR positively regulates DAP biosynthesis in <i>S. roseosporus</i>. The in-frame deletion of the <i>dhyR</i> gene resulted in a significant downregulation of the transcription levels of all structural genes within the DAP biosynthetic gene cluster and a significant decrease in DAP yield. In contrast, overexpression of <i>dhyR</i> enhanced the transcription levels of the DAP biosynthetic gene cluster, leading to a 23% increase in DAP yield. Deletion of <i>dhyR</i> caused significant changes in the expression of multiple genes involved in carbohydrate metabolism, energy metabolism and amino acid metabolic pathways through transcriptome analysis. Especially, deletion of <i>dhyR</i> led to a significant downregulation of transcription levels of three DAP biosynthesis-associated genes, including <i>atrA</i>, <i>depR1</i> and <i>ssig-05090</i>. In summary, DhyR positively regulates DAP biosynthesis in <i>S. roseosporus</i> by influencing the expression of the DAP gene cluster and modulating precursor flux. It functions as a pleiotropic regulator of primary and secondary metabolism in <i>S. roseosporus</i>.</p>","PeriodicalId":209,"journal":{"name":"Microbial Biotechnology","volume":"18 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-7915.70110","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Scrofuloderma of the Intermammary Area in an Adolescent Female: A Case Report","authors":"Mandeep Dutta Joshi, Om Prakash Bhatta, Arun Kalikote, Surendra Sapkota, Avinash Yadav, Ajay Kumar","doi":"10.1002/ccr3.70285","DOIUrl":"https://doi.org/10.1002/ccr3.70285","url":null,"abstract":"<p>Scrofuloderma, a rare form of cutaneous tuberculosis (CTB), typically arises from underlying tuberculous lymphadenitis. Although CTB comprises only 1%–2% of extrapulmonary cases, its burden may be underestimated in endemic countries, such as Nepal, leading to delayed diagnosis and increased morbidity. We present the case of a 14-year-old girl with a painless, ulcerated lesion in the intermammary region for 3 months, histopathologically diagnosed as scrofuloderma.</p>","PeriodicalId":10327,"journal":{"name":"Clinical Case Reports","volume":"13 3","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ccr3.70285","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Efficacy of prostatectomy using a Retzius-sparing approach as an additional excision in anal canal carcinoma after brachytherapy for prostate adenocarcinoma","authors":"Takahiro Yanase, Taku Naiki, Aya Naiki-Ito, Ryosei Okawa, Sosuke Niwa, Nobuhiko Shimizu, Shuzo Hamamoto, Yoshiaki Fujii, Hiroki Takahashi, Takahiro Yasui","doi":"10.1002/iju5.70003","DOIUrl":"https://doi.org/10.1002/iju5.70003","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Standard treatments for squamous cell carcinoma of the anal canal include chemoradiotherapy and surgical resection. We report a rare case of squamous cell carcinoma of the anal canal with prostate invasion treated with curative surgical methods using a Retzius-sparing robot-assisted approach.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Case presentation</h3>\u0000 \u0000 <p>A 76-year-old male had undergone brachytherapy for prostate adenocarcinoma (cT2aN0M0). Nine years later, the patient was diagnosed with anal canal carcinoma (cT2N0M0) following the onset of anal pain. During a robot-assisted abdominoperineal resection, prostate invasion was suspected and a prostatectomy was performed as an additional excision of the prostate using a Retzius-sparing approach. Although an anastomotic leak was observed postoperatively, it was conservatively managed with bilateral ureteral stent placement. The patient remained recurrence-free after surgery.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>A Retzius-sparing robot-assisted approach in radical prostatectomy may be a safe and feasible option for cases of anal canal carcinoma with suspected prostate invasion.</p>\u0000 </section>\u0000 </div>","PeriodicalId":52909,"journal":{"name":"IJU Case Reports","volume":"8 2","pages":"174-178"},"PeriodicalIF":0.0,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/iju5.70003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}