Front Cover: Microfluidic-Assisted Evolution of a Robust NAD+-Dependent Enzyme with Improved Isobutanol Tolerance at Elevated Temperatures (ChemSusChem 20/2025)

IF 6.6 2区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

ChemSusChem Pub Date : 2025-10-23 DOI:10.1002/cssc.70229

Mariko Teshima, Robert Genth, Tenuun Bayaraa, Manuel Döring, Barbara Beer, Gerhard Schenk, Volker Sieber

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Abstract

The Front Cover shows a microfluidic chip that was used in an enzyme engineering approach utilizing microdroplet-based UHTS to optimize an aldehyde dehydrogenase towards higher temperature stability and solvent tolerance. The optimized enzyme was then applied as part of a multi-enzyme cascade as indicated by the circle of enzyme structures above the chip. The ultimate goal is a continuous process for the conversion of glucose into isobutanol in a two-phase system using these enzymes and based on the formation of an isobutanol phase at sufficiently high concentration and with continuous removal of the organic phase, as shown by the reactor in the background. More information can be found in the Research Article by V. Sieber and co-workers (DOI: 10.1002/cssc.202501120).

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封面：微流体辅助进化稳健的NAD+依赖酶，在高温下提高异丁醇耐受性（ChemSusChem 20/2025）

前盖展示了一种微流控芯片，该芯片用于酶工程方法，利用基于微滴的超高温超导技术优化醛脱氢酶，使其具有更高的温度稳定性和溶剂耐受性。然后将优化的酶作为多酶级联的一部分，如芯片上方的酶结构圈所示。最终目标是利用这些酶在两相系统中将葡萄糖转化为异丁醇的连续过程，并以足够高浓度的异丁醇相的形成为基础，并连续去除有机相，如背景中的反应器所示。更多信息可以在V. Sieber及其同事的研究文章中找到（DOI: 10.1002/cssc.202501120）。

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来源期刊

ChemSusChem 化学-化学综合

CiteScore

15.80

自引率

4.80%

发文量

555

审稿时长

1.8 months

期刊介绍： ChemSusChem Impact Factor (2016): 7.226 Scope: Interdisciplinary journal Focuses on research at the interface of chemistry and sustainability Features the best research on sustainability and energy Areas Covered: Chemistry Materials Science Chemical Engineering Biotechnology