Wiley最新文献

{"title":"Isolation and Characterization of Seneca Valley Virus From Pig Transboundary Spread to the Mink Infection","authors":"Ziliang Qin,&nbsp;Xinmiao He,&nbsp;Chao Chen,&nbsp;Shaojun Chen,&nbsp;Zida Nai,&nbsp;Yao Wang,&nbsp;Wentao Wang,&nbsp;Gang Li,&nbsp;Fang Wang,&nbsp;Ming Tian,&nbsp;Haijuan He,&nbsp;Heshu Chen,&nbsp;Di Liu,&nbsp;Xinpeng Jiang","doi":"10.1155/tbed/4428550","DOIUrl":"https://doi.org/10.1155/tbed/4428550","url":null,"abstract":"<div>\u0000 <p>Seneca Valley virus (SVV) infection has recently disseminated across pig farms in Canada, America, and China. The SVV has been identified in humans, rodents, and houseflies. Although cross-species transmission events may lead to limited subsequent transmission, sustained outbreaks have been observed in new mammalian hosts. Thus, in our study, we utilized molecular characteristics, pathological examination, and the immune response to ascertain whether mink could serve as a novel mammalian host for SVV. Here, our study utilized a porcine strain of SVV to infect minks orally, resulting in pathological changes in the intestines. In addition, SVV could stimulate a specific neutralizing antibody response. The neutralizing antibody against SVV has also been found in mink through an epidemiological investigation in Heilongjiang Province. This study highlights the role of SVV infection in minks as an impetus for viral evolution, which poses potential threats to livestock, public health, and economic prosperity.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/4428550","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Global Assessment of Plant and Animal Community Responses to Forest Management Over Time","authors":"Hanneke van 't Veen,&nbsp;Koen Kuipers,&nbsp;Aafke Schipper,&nbsp;Alexandra Marques,&nbsp;Mart-Jan Schelhaas,&nbsp;Rob Alkemade","doi":"10.1111/gcb.70279","DOIUrl":"https://doi.org/10.1111/gcb.70279","url":null,"abstract":"<p>Transitions to forest management that mitigate negative effects of forest use on biodiversity are required to bend the curve of biodiversity loss. To facilitate such transitions, it is vital to understand the effects of different forest management practices on biodiversity. In this study, we analyzed observation data of 182 studies (312,453 abundance values) from three global biodiversity databases to estimate the effects of seven types of forest management on plant and animal (i.e., insects and vertebrates) biodiversity over time, and on threatened animals. We captured biodiversity in four distinct metrics (i.e., quantifiable measures of community composition): (i) intactness, (ii) relative species richness, (iii) compositional similarity, and (iv) relative total abundance, all calculated as the difference in biodiversity between managed and undisturbed forests. Overall, we find larger effects of forest management on intactness and similarity compared to richness and abundance. This suggests that forest management leads to a loss of species that specifically occur in undisturbed forests but that this decline is partially offset by an influx of species from other areas. We find that extensive forest management, such as selective cutting and agroforestry, supports higher levels of overall and threatened biodiversity than intensive management, such as forest and perennial tree crop plantations. We also find significant increases in animal community intactness and similarity in agroforests and forest plantations over 40 and 80 years since establishment, respectively, but do not find this for relative species richness and total abundance. This indicates that animal communities in these management systems become increasingly similar to those of undisturbed forests. Overall, our results highlight the potential of selective cutting and agroforests to mitigate biodiversity loss compared to more intensive systems, as well as the potential of longer rotation periods in forest plantations to increase habitat availability for species specifically adapted to undisturbed forests.</p>","PeriodicalId":175,"journal":{"name":"Global Change Biology","volume":"31 6","pages":""},"PeriodicalIF":10.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gcb.70279","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Data-Driven Solution for Large-Scale Open-Pit Mines Excavation Monitoring Based on 3D Point Cloud","authors":"Taiming He,&nbsp;Jiasui Zhang,&nbsp;Lu Yang","doi":"10.1049/ipr2.70130","DOIUrl":"https://doi.org/10.1049/ipr2.70130","url":null,"abstract":"<p>We present an adaptive point cloud workflow that withstands heavy environmental noise and the large datasets typical of open-pit mines. The workflow automatically tunes its parameters from the statistics of each input scene, eliminating manual parameter tuning. For instance, it sets the ICP correspondence distance and the clustering threshold without user input. Additionally, our method integrates a coarse-to-fine registration strategy, robust change detection, and precise volumetric estimation based on digital elevation models. Experiments on simulated mining datasets show our method remains robust under heavy noise and misalignment, with volume errors consistently below <span></span><math>\u0000 <semantics>\u0000 <mrow>\u0000 <mn>2</mn>\u0000 <mo>%</mo>\u0000 </mrow>\u0000 <annotation>$2%$</annotation>\u0000 </semantics></math>. A field pilot study at a limestone quarry further underscores its practical reliability and operational robustness. This research provides a precise, automated solution for real-time mining monitoring, effectively advancing sustainable and intelligent mining practices. Source code and datasets are publicly available at github.com/deemoe404/volcal_baseline.</p>","PeriodicalId":56303,"journal":{"name":"IET Image Processing","volume":"19 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/ipr2.70130","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"计算机科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Allyl Isothiocyanate Addition on Consumers’ Sensory Perception in a Solid Food Matrix","authors":"Emily Dolan,&nbsp;Nicoletta Faraone,&nbsp;Matthew B. McSweeney","doi":"10.1111/1750-3841.70336","DOIUrl":"https://doi.org/10.1111/1750-3841.70336","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p>Cross-modal interactions have been investigated for different chemical irritants, but allyl isothiocyanate (AITC) remains largely unexplored. The objective of this study was to evaluate AITC's cross-modal interactions and impact on sensory perception of a solid food matrix (i.e., curried rice) when added at detection threshold (0.123 mg/100 mL) and below detection threshold (0.115 mg/100 mL). Furthermore, the study investigated the impact of encapsulation of AITC (spray dried with gum Arabic and Tween-20) on sensory perception. Curried rice was prepared in five variations: without AITC, with AITC added at and below the detection threshold, and with encapsulated AITC added at and below the detection threshold. The participants (<i>n</i> = 88) evaluated the samples using hedonic scales and rate-all-that-apply. The encapsulated AITC did not impact the consumers' liking scores, but the unencapsulated AITC significantly decreased their liking of texture and overall liking. The encapsulated AITC only increased bitterness and spiciness when added at the detection threshold and did not suppress the sweetness. Both forms of AITC at the detection threshold increased the saltiness perception of the curried rice. The unencapsulated AITC added at both concentration levels suppressed the sweetness of the curried rice and increased the intensity of bitterness and spiciness. Below the detection threshold, neither form of AITC increased saltiness perception. Future studies should continue to explore AITC in different food matrices, using different encapsulation methods and using dynamic sensory methodologies to fully explore its impact on sensory perception of foods.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Practical Application</h3>\u0000 \u0000 <p>The food industry is investigating new methods to increase saltiness perception of food items, while reducing sodium content and salt. This study evaluated how AITC can be used to enhance the saltiness perception of a solid food. Furthermore, it evaluated the impact of encapsulation of AITC's cross-modal interactions. By investigating the addition of AITC and encapsulated AITC, this study helps identify how the addition of AITC impacts sensory perception, as well as if it is a suitable candidate to enhance the saltiness perception of food.</p>\u0000 </section>\u0000 </div>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1750-3841.70336","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cover Feature: C3-Symmetric Chiral Cage-Shaped Phosphates: Synthesis and Application as Organocatalysts in Asymmetric Iodolactonizations (Asian J. Org. Chem. 6/2025)","authors":"Xiao Liu,&nbsp;Dr. Akihito Konishi,&nbsp;Prof. Dr. Makoto Yasuda","doi":"10.1002/ajoc.202580602","DOIUrl":"https://doi.org/10.1002/ajoc.202580602","url":null,"abstract":"<p>The <i>C</i>₃-symmetric, cage-shaped phosphate creates a helical chiral environment that functions like a vortex with a single direction. When a linear starting material enters this chiral environment, it is transformed into a precisely controlled stereochemical product. This transformation represents how the chiral vortex causes a dragon to curl up into a coil! The cover image depicts the dragon's transformation, which is catalyzed by our vortex-shaped catalyst. More details can be found in article number e202500299 by Xiao Liu, Akihito Konishi, and Makoto Yasuda. \u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":130,"journal":{"name":"Asian Journal of Organic Chemistry","volume":"14 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ajoc.202580602","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Ethiopian sorghum germplasm for resistance to anthracnose and performance for desirable agronomic traits","authors":"Chemeda Birhanu,&nbsp;Tesfaye Mengiste,&nbsp;Dagnachew Lule,&nbsp;Firew Mekbib,&nbsp;Zelalem Bekeko,&nbsp;Alemu Tirfessa,&nbsp;Pascal Okoye,&nbsp;Getachew Ayana,&nbsp;Gezahegn Girma,&nbsp;Habte Nida,&nbsp;Abera Takele","doi":"10.1002/agg2.70138","DOIUrl":"https://doi.org/10.1002/agg2.70138","url":null,"abstract":"<p>Anthracnose, caused by the fungus <i>Colletotrichum sublineola</i>, is an economically important disease of sorghum [(<i>Sorghum bicolor</i> (L.) Moench] in warm and humid regions of Ethiopia. Frequent screening for resistance sources and the development of resistant varieties is crucial for disease management, but achieving resistance has been challenging due to the high variability of the pathogen. A total of 358 Ethiopian sorghum landraces were evaluated to identify anthracnose resistance and other desirable agronomic traits under natural infestation in the field and inoculation of plants in the greenhouse. Field evaluations were conducted at Asosa, Bako, Jimma, and Haramaya in Ethiopia for two growing seasons. Greenhouse screening was performed at Purdue University using a mixture of five strains of <i>C. sublineola</i>. Under field conditions, area under disease progress curve was used to determine anthracnose disease severity. In addition, major agronomic traits such as panicle weight, panicle yield, grain yield, and thousand-grain weight were recorded. Combined analysis of variance revealed highly significant (<i>p</i> &lt; 0.01) differences among accessions, locations, and genotype-by-environment interactions for disease parameters and agronomic traits. We identified 71 accessions showing consistent resistance, developing disease severity levels between 12% and 30% across locations. The analysis of greenhouse multi-strain screenings also revealed significant genetic variation with 33 accessions exhibiting resistant reactions without showing any disease symptoms. Overall, 55 accessions showed resistant reactions to anthracnose both under fields and greenhouse evaluations. The accessions with low anthracnose severity and high grain yield with desirable traits are valuable for future sorghum improvement programs.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"8 2","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70138","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimating biomass and carbon stock in orange trees (Citrus sinensis L. Osbeck) of the São Paulo and southwestern Minas Gerais citrus belt, Brazil","authors":"Lauro Rodrigues Nogueira Jr.,&nbsp;Carlos Cesar Ronquim,&nbsp;José Carlos Barbosa,&nbsp;Vinicius Gustavo Trombin,&nbsp;Roseli Reina,&nbsp;Fernando Alvarinho Delgado,&nbsp;Fernando Antônio de Pádua Paim","doi":"10.1002/agg2.70147","DOIUrl":"https://doi.org/10.1002/agg2.70147","url":null,"abstract":"<p>The living biomass and carbon stock of <i>Citrus sinensis</i> (L. Osbeck) orchards from the citrus belt in the states of São Paulo and southwestern Minas Gerais, Brazil, is receiving increasing attention due to its role in the carbon balance of citrus production and in climate change mitigation. Orange trees were analyzed to develop allometric equations to estimate the carbon stock of living biomass at citrus orchards in Brazilian citrus belt. Above- and belowground living biomass and biometric variables were measured directly for 80 harvested orange trees, considering the Pera and Valencia orange varieties and four age classes (3–5, 6–10, 11–15, &gt;15-year old). Considering the 80 orange trees evaluated by direct method, the biomass ranged between 22 and 224 kg tree⁻¹, with the branches (54%) being the main compartment, followed by the roots (28%), leaves (10%), and trunk (8%). Allometric equations were developed using stepwise backward regression analysis. For carbon stock estimation, the allometric equation was applied in a sample of 1321 orange trees distributed inside the citrus belt studied. This sample represents the 162 million orange trees that are more than 3 years old in 337,091 ha, and has stocked more than 8.4 million Mg C in the living biomass. We show for the first time that orange tree biomass can be estimated by allometric equations and that these equations can be used to estimate the biomass of orange trees, and can be recommended for carbon biomass inventories for similar regions, orchards, tree size ranges, and site characteristics.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"8 2","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70147","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term benefits of single-dose psilocybin in depressed patients with cancer","authors":"Manish Agrawal MD,&nbsp;Kim Roddy MBA,&nbsp;Betsy Jenkins MS,&nbsp;Celia Leeks,&nbsp;Ezekiel Emanuel MD, PhD","doi":"10.1002/cncr.35889","DOIUrl":"https://doi.org/10.1002/cncr.35889","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Patients with cancer often struggle with depression, which can negatively impact quality of life as well as be challenging to manage.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A phase 2 trial was conducted that demonstrated safety, feasibility, and efficacy of a single dose of psilocybin combined with psychological support in a community cancer setting in 30 patients with cancer and a major depressive disorder. Here, efficacy outcomes at 2 years’ follow-up are reported.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Of 28 patients, 15 (53.6%) demonstrated significant reduction in depression as measured by the Montgomery Asberg Depression Rating Scale (average, –15.0 points from baseline; <i>p</i> &lt; .001), and 14 (50%) had sustained depression reduction. Thirteen patients (46.4%) experienced significant reduction in anxiety as measured by the Hamilton Anxiety Rating Scale (average, –13.9 points from baseline, <i>p</i> &lt; .001), and 12 (42.9%) had sustained anxiety reduction.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>These findings demonstrate robust antidepressive activity from a single 25 mg dose of psilocybin combined with psychotherapy and suggest a potentially paradigm-changing alternative to traditional antidepressants requiring further study.</p>\u0000 </section>\u0000 </div>","PeriodicalId":138,"journal":{"name":"Cancer","volume":"131 12","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traditional and Emerging Physical Processing Technologies: Applications and Challenges in Allergen Control of Animal and Plant Proteins","authors":"Lili Zhang,&nbsp;Vijaya Raghavan,&nbsp;Jin Wang","doi":"10.1111/1541-4337.70196","DOIUrl":"https://doi.org/10.1111/1541-4337.70196","url":null,"abstract":"<p>Under global food shortages and environmental challenges, the food industry is shifting from animal to plant proteins, coinciding with a rising prevalence of food allergies. Structural and functional differences between animal and plant proteins significantly influence their allergenic potential. Traditional and emerging physical processing technologies can modify protein structures and reduce immunoreactivity without chemical additives, preserving food quality. These technologies are categorized into thermal, radiation, light, high-pressure, transient pressure, mechanical wave, plasma, and electric field effects. Cold plasma is particularly effective in modifying protein structures by generating reactive species. High hydrostatic pressure also demonstrates significant potential, particularly when combined with heat treatment. Ultrasound, especially when integrated with other methods (e.g., enzymatic hydrolysis or glycosylation), offers versatile and cost-effective solutions. Microwave heating remains a practical option due to its efficiency and scalability, while gamma irradiation remains an effective tool for allergen reduction despite its regulatory and consumer acceptance challenges. Low-allergenic processed foods can induce oral tolerance in allergic individuals, necessitating comprehensive safety evaluations covering allergenicity, toxicity, and nutrition. Unintentional allergens in food processing pose risks, and current regulations are insufficient. International organizations have proposed precautionary allergen labeling to protect consumers. While processing technologies cannot fully eliminate allergenicity, optimized selection can mitigate risks. Future research should focus on their impact on functional properties, nutritional value, safety, cost, and production efficiency to ensure safe and effective application in the food industry.</p>","PeriodicalId":155,"journal":{"name":"Comprehensive Reviews in Food Science and Food Safety","volume":"24 4","pages":""},"PeriodicalIF":12.0,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1541-4337.70196","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of Arginine Metabolism by Transcription Factor Btn2p in a Mixed Culture of Saccharomyces cerevisiae and Pediococcus pentosaceus and Subsequent Ethanol Tolerance","authors":"Huqi Zhou,&nbsp;Ting Xia,&nbsp;Tangchao Chen,&nbsp;Anan Zhou,&nbsp;Qiqi Xia,&nbsp;Gongnian Xiao,&nbsp;Ruosi Fang","doi":"10.1111/1750-3841.70333","DOIUrl":"https://doi.org/10.1111/1750-3841.70333","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p>Ethyl carbamate (EC) is a natural carcinogen widely found in fermented alcoholic beverages. The compound is mainly generated through the reaction of urea and citrulline with ethanol. The transcription factor Btn2p may affect arginine metabolism, and thus regulate EC in different fermentation systems. Therefore, in this study, we investigated the effects of Btn2p on arginine metabolism in <i>Saccharomyces cerevisiae</i> in different culture systems and analyzed the potential regulatory mechanisms of EC formation. In addition, we studied the ethanol tolerance of <i>BTN2</i>-modified yeast to determine its applicability in huangjiu fermentation and to provide a theoretical basis for subsequent studies. We found that <i>BTN2</i> knockout inhibited two major EC precursors, and the inhibitory effect was better in mixed cultures with <i>Pediococcus pentosaceus</i>. In addition, <i>BTN2</i> knockout promoted the activities of urease and ornithine transcarbamoylase, but reduced the activity of arginine deiminase, which led to the reduction of urea and citrulline concentrations. The growth conditions of <i>BTN2</i>-modified strains under different ethanol concentrations were also studied for future applications in huangjiu fermentation. The results showed that <i>BTN2</i> overexpression promoted cell growth and increased ethanol tolerance, whereas <i>BTN2</i> knockout reduced the ethanol tolerance of cell. The findings indicated that Btn2p was involved in arginine metabolism, possibly through the regulation of urea and citrulline metabolism, and <i>BTN2-</i>knockout strains can be used as a potential target for EC reduction.</p>\u0000 </section>\u0000 </div>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}