Analytical Methods最新文献_第8页

Carbon-based nanozymes: catalytic mechanisms, performance tuning, and environmental and biomedical applications. 碳基纳米酶：催化机制，性能调整，以及环境和生物医学应用。

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-08-07 DOI: 10.1039/d5ay00997a

Hongying Ye, Yongquan Lai, Zhaodi Wu, Guizhen Li, Qingqing Hua, Wenyuan Zhu

{"title":"Carbon-based nanozymes: catalytic mechanisms, performance tuning, and environmental and biomedical applications.","authors":"Hongying Ye, Yongquan Lai, Zhaodi Wu, Guizhen Li, Qingqing Hua, Wenyuan Zhu","doi":"10.1039/d5ay00997a","DOIUrl":"10.1039/d5ay00997a","url":null,"abstract":"Carbon-based nanozymes-a significant subclass of nanomaterials mimicking natural enzyme catalytic functions-offer superior thermal/chemical stability and enhanced biocompatibility compared to natural enzymes and other nanozyme types, owing to their unique carbon matrix. This review comprehensively examines research progress in carbon-based nanozymes. It details their classification into three categories: (1) carbon nanozymes (e.g., fullerenes, graphene, carbon dots, and nanotubes), (2) heteroatom-doped variants (N, P, S, and Se), and (3) metal/metal oxide-supported types, including single-atom nanozymes with M-Nx-C sites. The primary catalytic mechanisms, focusing on peroxidase (POD)-like and superoxide dismutase (SOD)-like activities, alongside oxidase (OXD)-like and catalase (CAT)-like mechanisms, are discussed. Strategies for regulating nanozyme performance-such as size/morphology control, composition/structure tuning (doping and defect engineering), surface modification, biomolecular interactions, and external environment manipulation (pH and temperature)-are highlighted. The review emphasizes the broad applications of carbon-based nanozymes in environmental engineering (pollutant detection/degradation), biosensing (H2O2, biomolecules, antioxidants, and tumor markers), and biomedicine (antioxidant/anti-inflammatory therapy, tumor treatment, antibacterial/antiviral applications, and bioimaging). Finally, it addresses existing challenges, including relatively lower activity/specificity compared to natural enzymes, limited enzyme-mimicking types, unclear atomic-scale mechanisms, synthesis control difficulties, biocompatibility/safety concerns, and the need for standardized research frameworks. This overview underscores the immense potential and future research directions for carbon-based nanozymes.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"6264-6281"},"PeriodicalIF":2.6,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the origin traceability of Menthae Haplocalycis Herba in China based on multivariate data fusion combined with an artificial intelligence classification algorithm 基于多元数据融合结合人工智能分类算法的中国薄荷原产地溯源研究

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-08-07 DOI: 10.1039/D5AY00694E

Sichen Wang, Kewei Zhang, Tianfei Ma, Xiuqi Gan, Rao Fu, Yingtong Ren, Tulin Lu and Chunqin Mao

{"title":"Study on the origin traceability of Menthae Haplocalycis Herba in China based on multivariate data fusion combined with an artificial intelligence classification algorithm","authors":"Sichen Wang, Kewei Zhang, Tianfei Ma, Xiuqi Gan, Rao Fu, Yingtong Ren, Tulin Lu and Chunqin Mao","doi":"10.1039/D5AY00694E","DOIUrl":"10.1039/D5AY00694E","url":null,"abstract":"In this study, characteristics of Menthae Haplocalycis Herba (MHH) from different districts in China were analyzed by multidimensional data. High-performance liquid chromatography (HPLC) was used for the analysis of non-volatile indicator components, and a Heracles NEO ultra-fast gas phase electronic nose (UF-GC-e-nose) was used for the analysis of volatiles. In addition, computer vision techniques were used to determine the color and texture characteristics of samples. Besides the distinctive volatile components in different growing areas, 17 characteristic factors were screened by multivariate statistical analysis to identify the geographical origin of MHH. Moreover, the Whale Optimization Algorithm-Deep Belief Network (WOA-DBN) classification algorithm was developed and optimized in tracing the geographical producing area of MHH. The accuracy was significantly improved in comparison with regular discriminant analysis methods, such as principal component analysis (PCA) or partial least squares discriminant analysis (PLS-DA). This work provides a reference for food geographical origin traceability and quality assessment by constructing intelligent algorithms based on multidimensional data fusion.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 32","pages":" 6526-6538"},"PeriodicalIF":2.6,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144792983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic ternary MnO2/(rGO@Ag) nanocomposites for sensitive electrochemical dopamine detection† 协同三元二氧化锰/(rGO@Ag)纳米复合材料对多巴胺的电化学敏感检测。

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-08-07 DOI: 10.1039/D5AY00876J

Ankita K. Dhukate, Pramod A. Koyale, Sajid B. Mullani, Swapnajit V. Mulik, Navaj B. Mullani, Tukaram D. Dongale and Sagar D. Delekar

{"title":"Synergistic ternary MnO2/(rGO@Ag) nanocomposites for sensitive electrochemical dopamine detection†","authors":"Ankita K. Dhukate, Pramod A. Koyale, Sajid B. Mullani, Swapnajit V. Mulik, Navaj B. Mullani, Tukaram D. Dongale and Sagar D. Delekar","doi":"10.1039/D5AY00876J","DOIUrl":"10.1039/D5AY00876J","url":null,"abstract":"The development of high-performance electrochemical sensors is vital for the accurate and sensitive detection of neurochemicals such as dopamine (DA), a critical biomarker for neurological disorders. In this study, we report the fabrication of a novel ternary nanocomposite (MRA-10), composed of MnO2 nanosheets (NSs), silver nanoparticles (Ag NPs), and reduced graphene oxide (rGO). The nanocomposite was synthesized via a hydrothermal process followed by sonochemical integration and applied as a sensing layer on a glassy carbon electrode (GCE). Among the tested variants, MRA-10 (with 10 wt% rGO@Ag) exhibited the best electrochemical performance because of the synergistic interaction of the high surface area of MnO2, the excellent electrical conductivity of rGO, and the catalytic activity of Ag. Structural analyses confirmed a uniform Ag distribution and a strong interfacial contact between the components. Electrochemical studies using EIS, CV, and DPV revealed a remarkably low charge transfer resistance (178.45 Ω), high electroactive surface area (0.0803 cm2), and superior sensitivity of 353.20 μA μM−1 cm−2. The sensor demonstrated a broad linear range (10–500 μM) and a low detection limit (0.2615 μM) for DA, with excellent reproducibility (RSD = 1.41%) and long-term operational stability. The DA oxidation mechanism followed a two-electron adsorption-controlled process. This study introduces a robust, scalable, and cost-effective electrode platform for neurotransmitter sensing with promising clinical and environmental diagnostic applications.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 33","pages":" 6584-6597"},"PeriodicalIF":2.6,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144792984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent developments in reverse phase-dispersive liquid-liquid microextraction: a review. 反相分散液液微萃取研究进展综述。

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-08-07 DOI: 10.1039/d4ay00228h

Enilin de Mendonça de Azevedo, Paloma Konzgen Maciel, Ana Claudia Beduhn Luckow, Maiara Helena de Melo Malinowski, Bruno Meira Soares

{"title":"Recent developments in reverse phase-dispersive liquid-liquid microextraction: a review.","authors":"Enilin de Mendonça de Azevedo, Paloma Konzgen Maciel, Ana Claudia Beduhn Luckow, Maiara Helena de Melo Malinowski, Bruno Meira Soares","doi":"10.1039/d4ay00228h","DOIUrl":"10.1039/d4ay00228h","url":null,"abstract":"In recent years, reverse phase-dispersive liquid-liquid microextraction (RP-DLLME) has emerged as a highly versatile and efficient sample preparation method in analytical chemistry. This miniaturized extraction method distinguishes itself by employing a hydrophilic extractant solvent dispersed in a predominantly hydrophobic sample matrix, which is the inverse of conventional DLLME approaches. RP-DLLME offers numerous advantages, including significant solvent reduction, high enrichment factors, short extraction times, simplicity, cost-effectiveness, and minimal environmental impact, aligning with the principles of Green Analytical Chemistry (GAC). Additionally, RP-DLLME demonstrated remarkable versatility for the analysis of complex matrices with high organic content, such as edible oils, lubricating oils, and petroleum-derived samples. This review provides a comprehensive overview of the concepts, fundamentals, advantages, limitations, and advances of RP-DLLME applied to various samples types for elemental and organic analysis, covering studies published up to 2025.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"6282-6296"},"PeriodicalIF":2.6,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-throughput plasma/serum proteomics by coupling nanoparticle protein corona-based sample preparation and capillary zone electrophoresis-tandem mass spectrometry. 高通量血浆/血清蛋白质组学通过耦合纳米颗粒蛋白冠状样品制备和毛细管带电泳-串联质谱。

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-08-07 DOI: 10.1039/d5ay00721f

Qianyi Wang, Seyed Amirhossein Sadeghi, Fei Fang, Dejin Zheng, Chenxiang Luo, Guangyao Gao, Qianjie Wang, Bin Gu, Liangliang Sun

{"title":"High-throughput plasma/serum proteomics by coupling nanoparticle protein corona-based sample preparation and capillary zone electrophoresis-tandem mass spectrometry.","authors":"Qianyi Wang, Seyed Amirhossein Sadeghi, Fei Fang, Dejin Zheng, Chenxiang Luo, Guangyao Gao, Qianjie Wang, Bin Gu, Liangliang Sun","doi":"10.1039/d5ay00721f","DOIUrl":"10.1039/d5ay00721f","url":null,"abstract":"A high-throughput workflow for bottom-up proteomics (BUP) of human plasma using capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) and nanoparticle protein corona-assisted sample preparation is presented. The streamlined approach enabled the identification and quantification of hundreds of proteins from plasma/serum samples in 3.5 hours from sample to data. Nanoparticles with varied physiochemical properties studied in this work captured different pools of the plasma/serum proteome in the protein coronas, and the protein corona-based sample preparation approach enabled the measurement of low-abundance proteins compared to the approach without nanoparticles. Applying this high-throughput workflow to serum samples of a mouse NUT carcinoma (NC) cancer model allowed the determination of differentially expressed serum proteins between NC bearing mice and healthy controls. By comparing our quantitative proteomics data with published transcriptomics data, we revealed a handful of potential serum protein biomarkers of NC cancer (e.g., secreted phosphoprotein 1, SPP1). We expect this high-throughput workflow, with additional improvement in the speed of the mass spectrometer, will be useful for advancing the discovery of new protein biomarkers of diseases (e.g., cancer) using plasma/serum samples.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"6356-6365"},"PeriodicalIF":2.6,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12305297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative evaluation of positive and negative LC-MS modes for DOM profiling† LC-MS对DOM分析的阳性和阴性模式的比较评价。

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-08-07 DOI: 10.1039/D5AY00679A

Jiying Pei, Shiguo Chen, Jiayu Zhang, Wenfeng Yu, Weijie Qin and Kefu Yu

{"title":"Comparative evaluation of positive and negative LC-MS modes for DOM profiling†","authors":"Jiying Pei, Shiguo Chen, Jiayu Zhang, Wenfeng Yu, Weijie Qin and Kefu Yu","doi":"10.1039/D5AY00679A","DOIUrl":"10.1039/D5AY00679A","url":null,"abstract":"Dissolved organic matter (DOM) in marine environments is critical for nutrient cycling and carbon storage, forming a fundamental part of the ocean's biogeochemical cycles. Traditionally, DOM analysis has utilized negative electrospray ionization (ESI−) mass spectrometry, yet positive electrospray ionization (ESI+) mode has recently gained traction. However, few studies have compared ESI+ and ESI− to understand how these modes impact the overall DOM profiling results. This study investigates DOM profiles from the Sansha Yongle Blue Hole (SYBH)—the world's deepest blue hole—using HPLC-Orbitrap-MS/MS in both ESI+ and ESI− modes. Results show that ESI+ detects a broader range of DOM ions, with a significant correlation in ion counts between the two modes across different depths. ESI+ is more effective for detecting nitrogen-rich compounds (CHN, CHON, CHONS), while ESI− is better for sulfur-rich compounds (CHOS), especially in anoxic layers. Both modes identify similar levels of carbohydrates, carboxyl-rich alicyclic molecules (CRAM), and unsaturated aliphatic compounds (UA), but ESI+ detects more highly unsaturated compounds (HU), peptides, and polyphenols. Correlations in ion counts for carbohydrates, CRAM, and UA across depths indicate consistency between modes, while differences for HU, peptides, and polyphenols suggest lower ionization efficiency in ESI−. Both modes distinguish oxic, chemocline, and anoxic layers, but molecular parameters like double bond equivalents (DBE), modified aromaticity index (AImod), and H/C ratios correlate between modes, while O/C ratios do not. These discrepancies suggest that using only one ionization mode may bias DOM interpretation. Future studies should employ multi-mode ionization to enhance DOM profiling and understanding across marine environments.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 33","pages":" 6598-6608"},"PeriodicalIF":2.6,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144792981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple and economic solid-phase extraction method for purification of hesperidin from orange peel using a metal-organic framework-adsorbent (MIL-101 (Cr)). 采用金属-有机骨架吸附剂MIL-101 (Cr)，建立了一种简单、经济的固相萃取法纯化橙皮中橙皮苷。

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-08-07 DOI: 10.1039/d5ay00752f

Akram Sadat Nabavi, Sayed Mehdi Ghoreishi, Fateme Tajabadi

{"title":"A simple and economic solid-phase extraction method for purification of hesperidin from orange peel using a metal-organic framework-adsorbent (MIL-101 (Cr)).","authors":"Akram Sadat Nabavi, Sayed Mehdi Ghoreishi, Fateme Tajabadi","doi":"10.1039/d5ay00752f","DOIUrl":"10.1039/d5ay00752f","url":null,"abstract":"Orange peel is a rich source of nutrients and phytochemicals, widely used in medicines and foods. It contains flavonoids and antioxidants beneficial for health, including hesperidin (HES), a flavonoid abundant in citrus peels. This study used solid-phase extraction (SPE) and molecular spectrophotometry to extract and identify HES. The process is accurate, cost-effective, rapid, and environmentally friendly. HES was extracted using a hydroalcoholic method with MIL-101 (Cr), a metal-organic framework (MOF), as the adsorbent. MIL-101 (Cr) was prepared hydrothermally and characterized by Fourier transform infrared spectroscopy (FT-IR), field-emission scanning electron microscopy (FE-SEM), and X-ray diffraction (XRD). Experimental parameters such as sample concentration, adsorbent dosage, pH, and solvent type were optimized. HES was desorbed using acetonitrile, and absorption was measured at 285 nm. The limit of detection (LOD) and limit of quantification (LOQ) for HES were 0.145 and 0.530 μg mL-1, respectively. The method exhibited good reproducibility with less than 2% RSD. This study successfully demonstrated the use of MIL-101 (Cr) in HES extraction, offering a rapid, specific, accurate, and cost-effective analysis method.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"6397-6404"},"PeriodicalIF":2.6,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new electrochemical sensor for the detection of uric acid and xanthine based on a carbon paste electrode coated with a metal organic framework. 一种新型的用于检测尿酸和黄嘌呤的电化学传感器，该传感器基于涂覆金属有机框架的碳糊电极。

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-08-07 DOI: 10.1039/d5ay00832h

Ali Safaei, Susan Sadeghi

{"title":"A new electrochemical sensor for the detection of uric acid and xanthine based on a carbon paste electrode coated with a metal organic framework.","authors":"Ali Safaei, Susan Sadeghi","doi":"10.1039/d5ay00832h","DOIUrl":"10.1039/d5ay00832h","url":null,"abstract":"This study presents a simple strategy for developing a new electrochemical sensor based on a carbon paste electrode (CPE) coated with a copper nanotube-based MOF (NT Cu-MOF/CPE) using electrochemical/chemical approaches. This enzyme-free sensor was employed for the selective determination of uric acid (UA) and xanthine (XA) biomolecules in Britton-Robinson buffer (pH 3) individually and simultaneously by differential pulse voltammetry. The electrode surface was characterized using XRD, FE-SEM, EDX, and AFM techniques. Additionally, EIS and CV techniques were employed to investigate the electrochemical behavior of the prepared sensor. The obtained results indicated the facilitated oxidation of UA and XA on NT Cu-MOF/CPE compared to CPE. Under optimized conditions, the simultaneous determination of UA and XA showed a linear dependence of the sensor response on the analyte concentration in the range of 0.25-75.0 μM with detection limits of 0.10 and 0.25 μM for UA and XA, respectively. The sensor sensitivity for UA and XA was found to be 1.16 μA μM-1 cm-2 and 1.02 μA μM-1 cm-2, respectively. The repeatability of the sensor for six repeated measurements of UA and XA at a concentration of 10.0 μM was 1.3% and 0.5%, respectively. Finally, UA and XA were determined using the developed sensor in biological samples (urine and serum) with acceptable recoveries ranging from 93% to 106%. Furthermore, to assess the freshness of the meat, the XA content in fish meat at different storage times was determined using the developed sensor. The finding demonstrates the excellent potential of the developed sensor for clinical diagnostics and food preservation evaluation.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"6405-6419"},"PeriodicalIF":2.6,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a size exclusion chromatography cartridge-based analytical method for determination of free drug in nano-liposomal oncology drug formulations. 纳米肿瘤药物脂质体制剂中游离药物含量测定方法的建立。

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-08-07 DOI: 10.1039/d4ay02124j

Wei Zhang, Mark Paciolla, Lijun Duan, Elise Bradley, Aastha Chadha, Bhavesh Barot, Kaylee Worrell

{"title":"Development of a size exclusion chromatography cartridge-based analytical method for determination of free drug in nano-liposomal oncology drug formulations.","authors":"Wei Zhang, Mark Paciolla, Lijun Duan, Elise Bradley, Aastha Chadha, Bhavesh Barot, Kaylee Worrell","doi":"10.1039/d4ay02124j","DOIUrl":"10.1039/d4ay02124j","url":null,"abstract":"A reliable and stability-indicating size exclusion chromatography (SEC) cartridge-based free drug testing method was developed for active loading nanoliposome formulations through a systematic development approach. The SEC spin cartridge columns (7K MWCO, 2 mL) were used for developing the liposome free drug testing procedure. The SEC cartridge column retention capacity for a Mirati drug was determined (445 μg per cartridge). SEC testing conditions were studied to achieve a good separation between liposomes and the free drug, including cartridge conditioning, buffer wash steps for liposomal drug elution, organic media wash steps for free drug elution, and sample size effect. Qualification of this newly developed SEC cartridge method has demonstrated its specificity/selectivity without interference and excellent detection linearity (correlation coefficient (R) > 0.999) over a study concentration range (1.0 to 46.9 μg mL-1), sufficient LOQ (0.52 μg mL-1 equivalent to 1.7% of free drug in a liposome formulation at 2.5 mg mL-1), acceptable accuracy/recovery of 81 to 89% for free drug in spiked samples at 4.5%, 9%, and 18% levels (sample loading by a regular pipet) and 88 to 97% at 10% spike level (sample loading by a positive displacement pipet), good method precision (RSD (n = 6) of 4% NMT) for free drug determination, and 3 days solution stability for both standard and sample solutions (2-8 °C). In comparative sample testing, the SEC free drug test results were in good agreement with the solid phase extraction (SPE) test results for active loading formulations. The new method's stability-indicating ability has been proved through monitoring free drug in a liposome sample stored at -20 °C and -80 °C.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"6336-6347"},"PeriodicalIF":2.6,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Golgi-targeting fluorescent probe for the detection of hydrazine in biological and environmental systems. 用于生物和环境系统中肼检测的高尔基靶向荧光探针。

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-08-07 DOI: 10.1039/d5ay00690b

Hui-Juan Lai, Xu Wang, Yuan Wang, Wei-Na Wu, Zhi-Hong Xu

引用次数: 0