Analytical Methods最新文献_第7页

Serum direct SMOS-qPCR: a fast approach for miRNAs detection†

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-02-24 DOI: 10.1039/D4AY02280G

Guodong Zhao, Yanmiao Dai, Chenjing Xia, Ying Xue and Hongwei Xu

{"title":"Serum direct SMOS-qPCR: a fast approach for miRNAs detection†","authors":"Guodong Zhao, Yanmiao Dai, Chenjing Xia, Ying Xue and Hongwei Xu","doi":"10.1039/D4AY02280G","DOIUrl":"10.1039/D4AY02280G","url":null,"abstract":"This study presents a novel method for direct amplification of multiple microRNAs (miRNAs) from serum samples using Sensitive and Multiplexed One-Step RT-qPCR (SMOS-qPCR). The technique eliminates the need for separate miRNA extraction and purification steps, offering a streamlined approach for non-invasive early disease diagnosis. We optimized reaction conditions, including serum treatment methods and PCR system volumes, to enhance interference resistance and detection sensitivity. The optimized serum direct SMOS-qPCR demonstrated a detection limit as low as 6 × 103 copies per μL for single-target miRNA, with excellent amplification efficiency (R2 > 0.99). In multiplex detection, the method successfully quantified four miRNAs simultaneously, maintaining high sensitivity and reproducibility. Analysis of 20 clinical serum samples further validated the method's applicability for large-scale screening. Overall, this rapid, cost-effective, and user-friendly approach represents a significant advancement in miRNA detection technology, potentially facilitating earlier and more accessible disease diagnosis.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 10","pages":" 2335-2341"},"PeriodicalIF":2.7,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143481643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A compact flow-batch analyzer equipped with mini piezoelectric pumps and image-based volume control†

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-02-24 DOI: 10.1039/D4AY01800A

Ester Back da Trindade, Enny Priscila Gomes da Silva, Guilherme José de Paula Gonçalves and Alexandre Fonseca

{"title":"A compact flow-batch analyzer equipped with mini piezoelectric pumps and image-based volume control†","authors":"Ester Back da Trindade, Enny Priscila Gomes da Silva, Guilherme José de Paula Gonçalves and Alexandre Fonseca","doi":"10.1039/D4AY01800A","DOIUrl":"10.1039/D4AY01800A","url":null,"abstract":"Flow-batch analyzers demand meticulous volume control for successful application in quantitative determinations, with the incorporation of high-efficiency pumps and valves recommended for their construction. However, routine recalibrations are frequently needed to uphold the accuracy of manipulated volumes, highlighting the value of exploring new alternatives for volume control and measurement, with less sophisticated apparatus. In this work a compact Flow-Batch (FB) analyzer with piezoelectric micropumps was developed to perform standard addition calibration, incorporating image-based detection to perform volume control. The instrument was evaluated by quantitatively determining Cr(VI), NO2−, and Fe(II) in water samples, combining RGB-based colorimetry with established spectrophotometric methods. The results demonstrate that a comprehensive analysis with five standard additions can be completed in approximately 10 minutes, maintaining a suitable linear correlation (R2 > 0.99) and precision (0.4 ≤ RSD ≤ 12.1%). Recoveries between 90% and 105% for analyte levels below Brazilian regulatory limits underscore the accuracy of the proposed approach. The study confirms that digital image monitoring provides an elegant alternative for controlling solution volumes in FB systems, eliminating the need for more robust pumps with precisely controlled flow rates.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 11","pages":" 2456-2466"},"PeriodicalIF":2.7,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of amide-based molecular cages for the highly selective and sensitive detection of nicotine†

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-02-21 DOI: 10.1039/D5AY00206K

Mina Bagherifard, Amrit Kaur, Kamal E. S. Nassar, Neelam Tariq, Zois Syrgiannis and Ioannis Spanopoulos

引用次数: 0

Rapid antibiotic biosensors based on multiple molecular recognition elements

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-02-21 DOI: 10.1039/D4AY02212B

Feng Guo, Keren Chen, Jiaru Yang, Yifan Wu, Jiageng Cheng, Qian Yang, Longjiao Zhu, Jun Li and Wentao Xu

{"title":"Rapid antibiotic biosensors based on multiple molecular recognition elements","authors":"Feng Guo, Keren Chen, Jiaru Yang, Yifan Wu, Jiageng Cheng, Qian Yang, Longjiao Zhu, Jun Li and Wentao Xu","doi":"10.1039/D4AY02212B","DOIUrl":"10.1039/D4AY02212B","url":null,"abstract":"The extensive use of antibiotics poses significant public health concerns, including the increase in drug-resistant bacteria and environmental pollution, underscoring the urgent need for rapid, sensitive, and specific antibiotic detection methods. Most current reviews on antibiotic detection primarily focus on categorizing antibiotics based on their types or the classification of sensors used, such as electrochemical, optical, or colorimetric sensors. In contrast, this review proposes a novel and systematic theoretical framework for the detection of antibiotics using sensors using seven popular molecular recognition elements-antibodies, aptamers, microorganisms, cells, peptides, molecularly imprinted polymers (MIPs), metal–organic frameworks (MOFs) and direct recognition modalities and briefly discusses the mechanism of molecular recognition elements and antibiotic recognition. Additionally, it explores biosensors developed using these elements, offering a detailed analysis of their strengths and limitations in terms of sensitivity, specificity, and practicality. The review concludes by addressing current challenges and future directions, providing a comprehensive perspective essential for enhancing food safety and protecting public health.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 12","pages":" 2496-2514"},"PeriodicalIF":2.7,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid and accurate detection of huanglongbing in citrus by elasticity testing using a piezoelectric finger†

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-02-20 DOI: 10.1039/D4AY01952K

Pawan Rao, Shu Huang, Cheryl M. Armstrong, Joseph Capobianco, YongPing Duan, Wei-Heng Shih and Wan Y. Shih

{"title":"Rapid and accurate detection of huanglongbing in citrus by elasticity testing using a piezoelectric finger†","authors":"Pawan Rao, Shu Huang, Cheryl M. Armstrong, Joseph Capobianco, YongPing Duan, Wei-Heng Shih and Wan Y. Shih","doi":"10.1039/D4AY01952K","DOIUrl":"10.1039/D4AY01952K","url":null,"abstract":"Rapid and sensitive detection of citrus huanglongbing (HLB) is critical for the control of this devastating disease. In this study, we have evaluated using a piezoelectric finger (PEF) with a 0.4 mm probe to measure the elastic modulus of a leaf to detect HLB in four different species of citrus including grapefruit (GFT), pumelo (PUM), lemon (LEM), and Valencia orange (VAL). Diseased citrus leaves were harvested from trees testing positive for the presence of Candidatus Liberibacter asiaticus (Las), the causal agent of HLB, and included both symptomatic leaves, which were blotchy mottle or yellowing and asymptomatic leaves, which did not display outward symptoms. Healthy leaves were harvested from trees testing negative for Las. The results indicated that the PEF elastic modulus test exhibited an overall 94% sensitivity and 90% specificity against the Las status of the trees for all four citrus types combined. Comparative quantitative real-time polymerase chain reaction (qPCR) tests on the same leaves showed an overall 89% sensitivity and 100% specificity against the Las status of the trees. While a Cohen–Kappa coefficient of 0.81 was obtained between the PEF and qPCR predictions, suggesting a “strong” agreement between the PEF and qPCR tests, a more detailed examination indicated that PEF was more sensitive overall in detecting the Las positive trees than qPCR, particularly from asymptomatic leaves for which PEF was 96% sensitive versus 78% sensitive by qPCR, indicating the potential of using PEF for early detection of HLB.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 10","pages":" 2302-2311"},"PeriodicalIF":2.7,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ay/d4ay01952k?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D-printed electrodes for electrochemical detection of environmental analytes 用于电化学检测环境分析物的 3D 打印电极。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-02-20 DOI: 10.1039/D4AY02271H

Liangliang Pan, Shijing Zhou, Jiaying Yang, Tongyun Fei, Shuduan Mao, Li Fu and Cheng-Te Lin

{"title":"3D-printed electrodes for electrochemical detection of environmental analytes","authors":"Liangliang Pan, Shijing Zhou, Jiaying Yang, Tongyun Fei, Shuduan Mao, Li Fu and Cheng-Te Lin","doi":"10.1039/D4AY02271H","DOIUrl":"10.1039/D4AY02271H","url":null,"abstract":"Environmental monitoring faces increasing demands for rapid, sensitive, and cost-effective analytical methods to detect various pollutants. Three-dimensional (3D) printing technology has emerged as a transformative approach for fabricating electrochemical sensors, offering unprecedented flexibility in electrode design and potential for customization. This comprehensive review examines recent advances in 3D-printed electrochemical sensors for environmental analysis, focusing on manufacturing technologies, materials development, and surface modification strategies. We analyze various printing approaches, including fused deposition modeling, stereolithography, and selective laser melting, discussing their relative advantages and limitations for electrode fabrication. The review explores conductive materials development, from carbon-based composites to novel metal-containing filaments, and examines crucial surface modification techniques that enhance sensor performance. Key applications in environmental monitoring are evaluated, including the detection of heavy metals, pathogens, antibiotics, and organophosphates, with particular attention to analytical performance metrics and real-world applicability. Technical challenges are critically assessed, including limitations in printing resolution, material conductivity, and long-term stability. The review concludes by identifying promising research directions, such as the integration of advanced materials and the development of automated manufacturing processes, highlighting opportunities for improving sensor performance and commercial viability in environmental monitoring applications.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 10","pages":" 2235-2253"},"PeriodicalIF":2.7,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Development of a novel UHPLC-UV combined with UHPLC-QTOF/MS fingerprint method for the comprehensive evaluation of Nao-Luo-Xin-Tong: multi-wavelength setting based on traditional Chinese medicinal prescription composition

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-02-20 DOI: 10.1039/D5AY90017D

Lina Wang, Yanling Wang, Guangyun Tong, Yu Li, Mengnan Lei, Huan Wu, Bin Wang and Rongfeng Hu

引用次数: 0

A fast, accurate strategy based on LAMP–NALF for detecting human adenovirus serotype 3 in children

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-02-20 DOI: 10.1039/D4AY01973C

Qi Yun, Wen Hui Xia, Si Fei Ma, Dong Ming Lu, Meng Gu and Ao Shuang Zhu

{"title":"A fast, accurate strategy based on LAMP–NALF for detecting human adenovirus serotype 3 in children","authors":"Qi Yun, Wen Hui Xia, Si Fei Ma, Dong Ming Lu, Meng Gu and Ao Shuang Zhu","doi":"10.1039/D4AY01973C","DOIUrl":"10.1039/D4AY01973C","url":null,"abstract":" Objective: To establish a rapid, convenient and accurate visual detection protocol for human adenovirus serotype 3 in children's respiratory infections based on LAMP–NALF. Methods: Adenovirus serotypes prevalent in the region (Changzhou City, Jiangsu Province, China) were analyzed by assessing the results of tNGS analysis of respiratory pathogens in children. On this basis, multiple sets of LAMP primers were designed for the adenovirus hexon gene as the detection target. Optimal primer combinations were further screened, and the composition of the reagent system and amplification procedures were improved to achieve efficient amplification. To realize an instrument-independent, fast-testing strategy, by combining NALF, which is based on the principle of lateral chromatography, small molecule antigens were modified for primers. Moreover, amplification products were bound to the corresponding antibodies on nucleic acid chromatography test strips for visualization. Finally, a series of tests were performed to validate the sensitivity, specificity, and methodological consistency of the LAMP–NALF assay. Results: Adenovirus infections in children in this region during this period were mainly caused by serotype 3. The optimal amplification temperature for optimal primers of LAMP was 65 °C. The combination of LAMP and NALF could detect femtogram-grade positive plasmid of the hexon gene; besides, there was no cross-reactivity with five other types of common respiratory pathogens in children. The agreement between the LAMP–NALF assay and tNGS/qPCR for detecting the adenovirus in 100 respiratory clinical specimens was 98%. Conclusion: The combination of LAMP and NALF can achieve rapid, convenient and accurate detection of human adenovirus type 3, which can provide a powerful support for the pathogenic diagnosis of adenoviral infections in children, thus contributing to the promotion of the development of point-of-care testing for pathogens.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 10","pages":" 2295-2301"},"PeriodicalIF":2.7,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Strategies for the detection of site-specific DNA methylation and its application, opportunities and challenges in the field of electrochemical biosensors

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-02-20 DOI: 10.1039/D5AY90024G

Chenliu Ye, Zhibin Zhao, Penghui Lai, Chunmei Chen, Fumei Jian, Haiying Liang and Qiongying Guo

引用次数: 0

Development and validation of a combined QuEChERS and HPLC-MS/MS method for trace analysis of ten diamide insecticides in agricultural products†

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-02-19 DOI: 10.1039/D4AY02117G

Fajun Tian, Zhenzhen Zhou, Junfeng Lu, Chengkui Qiao, Caixia Wang, Tao Pang, Linlin Guo, Jun Li, Rongli Pang and Hanzhong Xie

{"title":"Development and validation of a combined QuEChERS and HPLC-MS/MS method for trace analysis of ten diamide insecticides in agricultural products†","authors":"Fajun Tian, Zhenzhen Zhou, Junfeng Lu, Chengkui Qiao, Caixia Wang, Tao Pang, Linlin Guo, Jun Li, Rongli Pang and Hanzhong Xie","doi":"10.1039/D4AY02117G","DOIUrl":"10.1039/D4AY02117G","url":null,"abstract":"Diamide insecticides are being widely registered worldwide, yet most of them lack established maximum residue limits (MRLs) in agricultural products. In this study, we combined a QuEChERS (quick, easy, cheap, efficient, rugged, and safe) extraction method with high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) analysis to simultaneously identify and quantify ten diamide insecticides in seven matrices for the first time. The method was validated in accordance with SANTE/11312/2021 guidelines, including sensitivity, linearity, trueness, and precision. Excellent linearity (R2 > 0.99) was obtained for all diamide insecticides within the concentration range of 5–1000 µg kg−1. The limit of detection (LOD) and limit of quantification (LOQ) were 0.01–1 µg kg−1 and 5 µg kg−1, respectively. The recoveries of the ten diamide insecticides at three levels (5, 100, and 1000 µg kg−1) ranged from 76.6% to 108.2% with good intra-day relative standard deviation (RSDr) (1.0–13.4%) and inter-day relative standard deviation (RSDR) (2.3–15.7%). The proposed method was applied to analyze 70 real agricultural product samples, and only six samples contained diamide insecticides. The results demonstrated that the method was both convenient and reliable for detecting diamide insecticides in agricultural products. The method was then applied to analyze agricultural product samples collected in a field trial to estimate the MRLs for the next step.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 10","pages":" 2282-2294"},"PeriodicalIF":2.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ay/d4ay02117g?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0