Analytical Methods最新文献

{"title":"Resolving severely overlapping ion mobility peaks using enhanced Fourier self-deconvolution†","authors":"Shujuan Liu, Jian Jia, Xiaoguang Gao and Xiuli He","doi":"10.1039/D4AY01854K","DOIUrl":"10.1039/D4AY01854K","url":null,"abstract":"<p >Fourier self-deconvolution is an effective method for resolving overlapping spectra. However, the selection of the half-width for the deconvolving function is often subjective, which can lead to either excessive convolution or insufficient resolution enhancement. Additionally, ion mobility peaks exhibit tailing effects, which may be misinterpreted as new peaks when the deconvolving function is modelled with a Gaussian function. This paper proposes an improved Fourier self-deconvolution method based on continuous wavelet transform. The proposed method determines the signal half-width by calculating the horizontal distance between the peaks and troughs of the wavelet coefficients, offering a more accurate estimation. Furthermore, an asymmetric function is employed to optimize the peak shape of the deconvolving function, significantly reducing the likelihood of peak misidentification. The effectiveness of the proposed method is validated using both simulated and experimental ion mobility spectrometry data. Experimental results demonstrate that the proposed method effectively enhances peak resolution and resolves overlapping peaks. Moreover, compared with other peak segmentation algorithms based on Fourier self-deconvolution, the proposed method demonstrates lower parameter estimation error and higher computational efficiency, particularly for severely overlapping peaks.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 2","pages":" 300-309"},"PeriodicalIF":2.7,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142764584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Establishment and characterization of noro-VLP measurement by digital ELISA","authors":"Takema Hasegawa, Yuriko Adachi, Kazumi Saikusa and Megumi Kato","doi":"10.1039/D4AY90160F","DOIUrl":"10.1039/D4AY90160F","url":null,"abstract":"<p >Correction for ‘Establishment and characterization of noro-VLP measurement by digital ELISA’ by Takema Hasegawa <em>et al.</em>, <em>Anal. Methods</em>, 2024, <strong>16</strong>, 7089–7094, https://doi.org/10.1039/D4AY01012D.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 2","pages":" 395-395"},"PeriodicalIF":2.7,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ay/d4ay90160f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142764579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multi-functional platform for stimulating and recording electrical responses of SH-SY5Y cells†","authors":"Fengyan Hou, Rui Wang, Xia Wang, Jianjun Dong, Tianzhu Yu, Qiuyang Deng and Zuobin Wang","doi":"10.1039/D4AY01515K","DOIUrl":"10.1039/D4AY01515K","url":null,"abstract":"<p >In recent years, multifunctional cell regulation on a single chip has become an imperative need for cell research. In this study, a novel multi-functional micro-platform integrating wireless electrical stimulation, mechanical stimulation and electrical response recording of cells was proposed. Controlling cell fate by photoexcited radio stimulation of cells on photosensitive films can precisely orchestrate biological activities. This is the first report of combining hydrogenated amorphous silicon (a-Si:H) photosensitive films and a 532 nm green laser for cell stimulation and electrical response recording. Remote wireless electrical stimulation of nerve cells through photoelectric effects based on photosensitive films evoked a change in membrane potential and promoted the neurite growth and neuronal differentiation. These effects were confirmed in a cell model of the human neuroblastoma cell line. The electrical response of cells demonstrated that the photocurrent generated by laser irradiation of the photosensitive film induced a redistribution of ions inside and outside the cell. Furthermore, a mechanical stimulus was applied to cells using a probe placed above them. The chip was used as a signal output to simultaneously obtain the electrical response of cells. The ability of photosensitive films to precisely excite cellular activity offers a novel prospect for wireless electrical stimulation. This work provides a promising strategy for the design of multi-functional biological devices based on a single chip.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 2","pages":" 275-283"},"PeriodicalIF":2.7,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combination of quadruple isotope dilution strategy and dispersive solid phase extraction method for accurate quantification of selected steroid hormones†","authors":"Neşe Ular Çağatay, Süleyman Bodur, Sezin Erarpat Bodur, Murat Emrah Maviş and Sezgin Bakırdere","doi":"10.1039/D4AY01545B","DOIUrl":"10.1039/D4AY01545B","url":null,"abstract":"<p >Steroid hormones are essential for the regulation of various vital body functions. Therefore, the determination of steroid hormones in biological fluids is a significant issue to find out the mechanism of steroid hormones related to disorders. In this study, Fe<small>₃</small>O<small>₄</small>/reduced graphene oxide (Fe<small>₃</small>O<small>₄</small>/rGO) nanocomposite based dispersive solid phase extraction (DSPE) was coupled with liquid chromatography – quadruple isotope dilution – triple quadrupole mass spectrometry (LC-ID<small>⁴</small>-MS/MS) for the accurate and precise determination of aldosterone, testosterone, 17-hydroxyprogesterone and estrone in serum samples. There is no study in literature about the combination of DSPE and LC-ID<small>⁴</small>-MS/MS methods for the extraction and determination of selected steroid hormones. Significant improvements in accuracy and precision for the proposed method was achieved by the combination of DSPE and LC-ID<small>⁴</small>-MS/MS. The percent recovery results were calculated between 100.0 and 103.4% with low percent relative standard deviation values (≤2.0%). The obtained recovery results proved the application of the developed method with high accuracy and precision for the determination of steroid hormones in serum samples. Hence, the developed method is superior to the current analytical methods for the determination of selected steroid hormones in terms of sensitivity, accuracy and precision.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 2","pages":" 256-264"},"PeriodicalIF":2.7,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A biomimetic 3D DNA nanoplatform for enhanced capture and high-purity isolation of stem cell exosomes†","authors":"Lingxia Meng, Tingting Zhao, Shuaiying Wang and Wenxiao Wang","doi":"10.1039/D4AY01665C","DOIUrl":"10.1039/D4AY01665C","url":null,"abstract":"<p >Exosomes are uniformly sized vesicle-like bodies that cells secrete. Researchers now believe that exosomes can mediate various health and pathological processes. However, because the biophysical properties of exosomes are similar to those of other cell secretion products and biological fluids are rich and diverse, their separation and purification have always been challenging. Inspired by the adhesive domains in the tentacles of marine organisms that effectively capture and release mobile food particles, we have built a biomimetic 3D DNA nanoplatform. This platform not only captures exosomes efficiently but also allows for light-controlled exosome release. The surface of the 3D DNA nanoplatform can grow multivalent aptamers <em>via</em> rolling circle amplification. Aptamers fold into specific secondary structures that bind to CD63, a protein expressed on the surface of exosomes, enabling efficient exosome capture. As a photothermal reagent, the temperature of the DNA nanoplatform increases under near-infrared light irradiation, destroying the secondary structure of the CD63 aptamer and releasing the exosomes. Additionally, we have demonstrated that a 3D DNA nanoplatform with multivalent CD63 aptamer structures achieves more efficient and convenient stem cell exosome separation compared to ultracentrifugation. This strategy provides an efficient and high-purity way to capture and reversibly separate exosomes, and the separated ultrapure exosomes are used for enhancing wound healing by modulating migration and angiogenesis.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 2","pages":" 388-394"},"PeriodicalIF":2.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of triazine herbicides in water, juice, and tea using deep eutectic solvent-based emulsive liquid–liquid microextraction combined with high-performance liquid chromatography†","authors":"Yulin Wang, Yuxin Wang, Yarong Chen, Xiaojiao Zheng, Jiajun Han, Xu Jing and Jiali Yang","doi":"10.1039/D4AY01823K","DOIUrl":"10.1039/D4AY01823K","url":null,"abstract":"<p >This study developed a deep eutectic solvent-based emulsive liquid–liquid microextraction method combined with high-performance liquid chromatography (DES-ELLME-HPLC) for the detection of triazine herbicides. The technique was validated for food sample detection. Hydrophobic DES was prepared by using tributylphosphine oxide and octanoic acid to replace toxic solvents. In ELLME, the prepared DES was mixed with distilled water and then added to samples for emulsification. During the emulsion, triazine herbicides are transferred from the sample to the extractant. The limit of detection was 0.008 mg L<small>⁻¹</small>, while the extraction recoveries ranged between 84.8% and 102.0%, exhibiting relative standard deviations between 0.3 and 4.9%. This pre-treatment technique is simple, fast, and green and can be applied to water, juice, and tea samples. Compared with traditional ELLME, DES pre-treatment solves the singularity of the extractant selection and allows the technique to be applied to multiple pesticides.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 2","pages":" 368-376"},"PeriodicalIF":2.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colorimetric aptasensors for sensitive low-density lipoprotein detection based on reduced oxide graphene@molybdenum disulfide-ferrocene nanosheets with peroxidase-like activity†","authors":"Guiyin Li, Tingting Yu, Haimei Li, Bingbing Wan, Xiaohong Tan, Xueqing Zhou, Jintao Liang and Zhide Zhou","doi":"10.1039/D4AY01648C","DOIUrl":"10.1039/D4AY01648C","url":null,"abstract":"<p >Low-density lipoprotein (LDL) is a key biomarker for cardiovascular disease (CVD) risk assessment. Monitoring LDL for the early diagnosis of CVD and its complications is an important clinical analysis tool. In this work, a novel colorimetric aptasensor for LDL detection was constructed <em>via</em> reduced graphene oxide@molybdenum disulfide-ferrocene-carboxylic nanosheets (rGO@MoS<small>₂</small>-Fc) with excellent peroxidase-like activity. On this basis, the LDL aptamer (LDLapt) immobilized on the surface of rGO@MoS<small>₂</small>-Fc served as a signal probe (rGO@MoS<small>₂</small>-Fc/LDLapt), while the unmodified LDLapt served as a capture probe. When LDL was present, it was recognized by the LDLapt and rGO@MoS<small>₂</small>-Fc/LDLapt to form an rGO@MoS<small>₂</small>-Fc/LDLapt/LDL/LDLapt sandwich-type conjugate with excellent enzymatic catalytic properties that can catalyze the generation of hydroxyl radicals (·OH) from hydrogen peroxide (H<small>₂</small>O<small>₂</small>), which in turn oxidized the colorless substrate <em>o</em>-phenylenediamine (OPD) to the yellow compound 2,3-diamino phenothiazine (DAP). In addition, the catalytic mechanism of the reaction was confirmed to be induced by ·OH through free radical experiments. The aptasensor had a linear range of 15.0 to 200.0 μg mL<small>⁻¹</small>, and a limit of detection (LOD) of 2.199 μg mL<small>⁻¹</small>. Overall, the assay has high selectivity, sensitivity and operability, showing broad application prospects in the clinical diagnosis of CVD.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 1","pages":" 136-144"},"PeriodicalIF":2.7,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction: NiFe2O4 nanoparticles decorated with MWCNTs as a selective and sensitive electrochemical sensor for the determination of epinephrine using differential pulse voltammetry","authors":"Ali A. Ensafi, F. Saeid, B. Rezaei and Ali R. Allafchian","doi":"10.1039/D4AY90152E","DOIUrl":"https://doi.org/10.1039/D4AY90152E","url":null,"abstract":"<p >Retraction of ‘NiFe<small>₂</small>O<small>₄</small> nanoparticles decorated with MWCNTs as a selective and sensitive electrochemical sensor for the determination of epinephrine using differential pulse voltammetry’ by Ali A. Ensafi <em>et al.</em>, <em>Anal. Methods</em>, 2014, <strong>6</strong>, 6885–6892, https://doi.org/10.1039/C4AY01232A.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 48","pages":" 8483-8483"},"PeriodicalIF":2.7,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/ay/d4ay90152e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142810787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of dimethylamine in low concentration particulate matter by reducing the concentration of 9-fluorenylmethyl chloroformate†","authors":"Susana García-Alonso, Francisco Javier Gómez-Moreno, Elisabeth Alonso-Blanco and Rosa María Pérez-Pastor","doi":"10.1039/D4AY00894D","DOIUrl":"10.1039/D4AY00894D","url":null,"abstract":"<p >This study presents a refined method that uses liquid chromatography with a fluorescence detector (LC-FD) to quantify trace amounts of dimethylamine in particulate matter (PM). This method was optimized to prioritize simplicity, cost-effectiveness and practicality. To ensure accurate and reliable analysis, strict protocols and procedures were followed to minimize cross-contamination. Separate workspaces were designated for preparing control blanks and sample treatments in one area and standard solutions in another, thus mitigating the risk of cross-contamination. An evaluation was conducted on different concentrations of 9-fluorenylmethyl chloroformate to derivatize dimethylamine. The results showed that a concentration of 3 μg mL<small>⁻¹</small> was effective in derivatizing dimethylamine concentrations up to 300 ng mL<small>⁻¹</small>. Increasing the concentration of the derivatization reagent from 2.9 to 7.3 μg mL<small>⁻¹</small> resulted in slightly elevated dimethylamine levels in blank measurements. Also, during the preparation of standards at low concentrations, high analytical coefficients of variation were observed. This highlights the importance of checking for potential sources of contamination. Method precision and quantification limits were evaluated through blank analysis, yielding values of approximately 20% and 20 ng mL<small>⁻¹</small>, respectively, consistent with chromatographic determination for environmental analysis. The suitability of the method for environmental analysis was demonstrated by analyzing eight PM<small>_2.5</small> samples. The concentrations of methylamine and dimethylamine were found to range from 0.8 to 3 ng m<small>⁻³</small> and 1.4 to 7.1 ng m<small>⁻³</small>, respectively, in accordance with the literature. Comparison with concurrent carbonyl measurements revealed similar concentration profiles. Both types of analyses can be performed using affordable methodologies that involve prior derivatization using a reduced concentration of the derivatization reagent.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 1","pages":" 145-154"},"PeriodicalIF":2.7,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ay/d4ay00894d?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the access to nature-identical vanillin via isoeugenol ozonation: in situ ATR-IR monitoring and safety evaluation†","authors":"Yun Zhao, Tingfei Li, Sisi Xie, Pingyi Zhang and Haifang Mao","doi":"10.1039/D4AY01306A","DOIUrl":"10.1039/D4AY01306A","url":null,"abstract":"<p >The transformation of renewable feedstocks into aromatic chemicals holds immense potential for advancing a green, low-carbon economy and fostering sustainable development. Herein, we present a novel approach for the conversion of isoeugenol, a renewable lignin derivative, into the valuable flavoring agent vanillin, utilizing ozone as an environmentally benign oxidant. The process optimization was significantly enhanced by the integration of <em>in situ</em> Attenuated Total Reflectance Infrared (ATR-IR) monitoring. The introduction of H<small>₂</small>O not only accelerated the decay of carbonyl oxides (Criegee intermediates) but also mitigated safety hazards stemming from the vigorous decomposition and heat release of secondary ozonides. Compared to the conventional Thin Layer Chromatography (TLC) method, ATR-IR monitoring demonstrated superior sensitivity and precision in determining the reaction endpoint, leading to a remarkable vanillin yield of 96.86% upon complete conversion of isoeugenol. Additionally, a comparative assessment of the sustainability of our approach with existing methods was undertaken, and valuable recommendations for safety assessments were provided to ensure the inherent safety of chemical engineering reactions. The present study serves as a pioneering effort in facilitating the implementation of a scalable, economically feasible and environmentally sustainable strategy for biomass flavor production, while contributing to the broader adoption of <em>in situ</em> spectroscopic technology within the larger economy.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 2","pages":" 310-319"},"PeriodicalIF":2.7,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142764586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}