{"title":"A supramolecular polypseudorotaxane material based on novel pillar[5]arene for ultrasensitive Fe3+ reaction†","authors":"Wen-Bo Zhu, Xiao-Wei Zhang, Zhi-Jun Li, Li-Yuan Liu, Yu-Quan Zhang, Liang Zhang and Hui Wen","doi":"10.1039/D4AY00832D","DOIUrl":"10.1039/D4AY00832D","url":null,"abstract":"<p >In this study, a novel linear supramolecular polypseudorotaxane was synthesized using a pillar[5]arene derivative (<strong>CP5</strong>) as the host and bis-bromohexyl pillar[5]arene (<strong>DP5</strong>) as the guest, facilitated by host–guest interactions. Subsequently, these components self-assembled to create an <strong>AIE</strong>-active material (<strong>CPDP-G</strong>) through C–H⋯π interactions involving the pillar[5]arene groups. Notably, the <strong>CPDP-G</strong> fluorescence material exhibits an exceptionally sensitive response to Fe<small><sup>3+</sup></small>, with a detection limit of 0.543 nM. The fabricated <strong>CPDP-G</strong> film serves as a rudimentary kit for Fe<small><sup>3+</sup></small> detection. Moreover, <strong>CPDP-G</strong> demonstrates robust stability under diverse conditions, including variations in temperature, exposure to KClO<small><sub>4</sub></small>, AcOH, and tetrabutylammonium hydroxide, and ultrasonic treatment. Consequently, the innovative supramolecular polypseudorotaxane material <strong>CPDP-G</strong> holds promising potential for the ultrasensitive detection of Fe<small><sup>3+</sup></small> ions.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 12","pages":" 2586-2590"},"PeriodicalIF":2.7,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mehdi Navaser, Ardeshir Shokrollahi, Fereshteh Zarghampour and Fatemeh Saeidi
{"title":"Enhancing gel electromembrane extraction using glycine-doped agarose to mitigate electroendosmosis flow: application to tramadol extraction from biological specimens","authors":"Mehdi Navaser, Ardeshir Shokrollahi, Fereshteh Zarghampour and Fatemeh Saeidi","doi":"10.1039/D4AY01939C","DOIUrl":"10.1039/D4AY01939C","url":null,"abstract":"<p >This study involves incorporating glycine into agarose to effectively eliminate electroendosmosis (EEO) effects in gel electromembrane extraction (G-EME) of tramadol (TMD) from biological samples. The development of a glycine-modified agarose membrane for EEO-free tramadol extraction represents a significant advancement in analytical chemistry with far-reaching societal implications. By eliminating the confounding effects of electroendosmosis and eschewing the use of harmful organic solvents typically employed in electromembrane procedures, this environmentally friendly method enhances the accuracy and precision of tramadol quantification in biological matrices. The operational procedure consists of the extraction tramadol, from 10 mL aqueous sample solution (pH 6.0), as the donor phase (DP), transferring it into membrane (pH 5.0); as the final step, it enters into an acceptor phase (AP, 700 μL, pH 4.0). The optimization of critical parameters, including the (w/v) percentage of agarose and glycine, the gel thickness, and the pH levels of the acceptor phase, gel, and donor phase, was conducted utilizing the one-factor-at-a-time methodology, while voltage, time, and stirring rate were optimized employing the design of experiment approach. Remarkably, under the optimized conditions, a satisfactory linear relationship was established within the range of 0.05–6 μg mL<small><sup>−1</sup></small> (<em>R</em><small><sup>2</sup></small> = 0.9925), accompanied by a commendable limit of detection (LOD) amounting to 0.015 μg mL<small><sup>−1</sup></small>, with an extraction recovery rate of 94%. Finally, the proposed method was successfully applied to the determination of TMD in urine, plasma, and tablet samples.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 11","pages":" 2398-2408"},"PeriodicalIF":2.7,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jian Zhang, Wen Yao, Xuanjiao Mao, Xinyu Hu, Li Lv and Haochen Qi
{"title":"A microfluidic DNA sensor array for real-time screening of early-stage lung cancer by simultaneous detection of multiple miRNAs†","authors":"Jian Zhang, Wen Yao, Xuanjiao Mao, Xinyu Hu, Li Lv and Haochen Qi","doi":"10.1039/D5AY00109A","DOIUrl":"10.1039/D5AY00109A","url":null,"abstract":"<p >We present a DNA sensor array for simultaneous detection of four targeted miRNAs in serum for early-stage lung cancer screening. Owing to effective microfluidic enrichment coupled with ultrasensitive solid–liquid capacitive sensing, the detection limit is as low as 12.84–24.69 aM, with a dynamic range of 0.1 fM to 10 pM. The response time for each miRNA detection is only 30 s, and the total detection time is 120 s. The selectivity reaches above 10 000 : 1. The miRNA concentrations in the patients with early-stage lung cancer are determined to be 6.5–263 times higher than those in healthy individuals, demonstrating the excellent feasibility of this sensor array for clinical applications for real-time screening of early-stage lung cancer.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 11","pages":" 2376-2379"},"PeriodicalIF":2.7,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jolly Kaushal, Sain Singh, Heena, Saakshi Saini and Partha Roy
{"title":"An easily synthesizable naphthalene-based sensing platform for Al3+ and Zn2+ ions: theoretical insights and live cells imaging†","authors":"Jolly Kaushal, Sain Singh, Heena, Saakshi Saini and Partha Roy","doi":"10.1039/D5AY00044K","DOIUrl":"10.1039/D5AY00044K","url":null,"abstract":"<p >A new naphthalene-derived Schiff base probe, 1-((2-(diphenylphosphino)ethylimino)methyl)naphthalen-2-ol (<strong>HL</strong>), has been demonstrated for fluorometric detection of Al<small><sup>3+</sup></small> and Zn<small><sup>2+</sup></small> ions. <strong>HL</strong> was characterized by elemental analysis, FT-IR, NMR, UV-Vis, fluorescence, and ESI-MS analyses. It exhibited high sensitivity and selectivity toward Al<small><sup>3+</sup></small> and Zn<small><sup>2+</sup></small> ions in a semi-aqueous medium (CH<small><sub>3</sub></small>CN–H<small><sub>2</sub></small>O; 4 : 1, v/v), remaining unaffected by other competing metal ions. As a ‘turn-on’ fluorogenic probe, <strong>HL</strong> displayed strong emission enhancements at 430 nm and 450 nm (<em>λ</em><small><sub>ex</sub></small> 300 nm) upon the addition of Al<small><sup>3+</sup></small> and Zn<small><sup>2+</sup></small> ions, respectively, with detection limits of 0.62 μM for Al<small><sup>3+</sup></small> and 0.54 μM for Zn<small><sup>2+</sup></small>. The addition of Al<small><sup>3+</sup></small> caused <em>ca.</em> 20 nm blue-shift in emission and absorption maxima of <strong>HL</strong> due to strong complex formation. The calculated binding constant values were found to be 1.39 × 10<small><sup>3</sup></small> M<small><sup>−1</sup></small> and 4.78 × 10<small><sup>3</sup></small> M<small><sup>−1</sup></small>, respectively, for Al<small><sup>3+</sup></small> and Zn<small><sup>2+</sup></small> ions. Job's plot, NMR, ESI-MS, and density functional theory (DFT) studies supported the metal ion binding mechanism with 1 : 1 stoichiometry. Fluorescence imaging experiments further revealed <strong>HL</strong>'s ability to detect intracellular Al<small><sup>3+</sup></small> in live cells with very low cytotoxicity, highlighting its potential as a selective chemosensory probe.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 12","pages":" 2541-2549"},"PeriodicalIF":2.7,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A high-density 4-vinylpyridine membrane for rapid purification of antibodies†","authors":"Qingrong Wu, Bin Wang and LiYun Zhang","doi":"10.1039/D4AY02100B","DOIUrl":"10.1039/D4AY02100B","url":null,"abstract":"<p >In order to further investigate the mechanism of action of rituximab in humans and its biotransformation, it is essential to obtain high purity rituximab while maintaining its structural and functional integrity. In this study, we proposed and fabricated a small-molecule 4-vinylpyridine-modified high-density membrane material (P4VP@M) using the surface-initiated atom transfer radical polymerization (SI-ATRP) method. This approach can overcome the limitations of conventional enrichment materials, such as long incubation time and low adsorption capacity. P4VP@M incorporates high-density HEMA polymer brushes to prevent non-specific protein adsorption and 4-vinylpyridine polymer brushes for high-performance rituximab enrichment under mild conditions. In addition, P4VP@M has high permeability, hydrophilicity and mechanical strength. Compared to previous methods, this novel material showed excellent rituximab binding capacity (100.3 mg g<small><sup>−1</sup></small>), shorter processing time (only 15 min), and lower cost (only $2). Notably, P4VP@M was able to purify rituximab rapidly and efficiently from the mixture samples and exhibited good selectivity, recovery (>92.6%) and purity (>94.8%). Thus, this proposed purification strategy using P4VP@M has great potential in enriching target proteins from complex samples, as well as contributing to the in-depth study of their biological functions.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 12","pages":" 2577-2585"},"PeriodicalIF":2.7,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Haitao Xu, Chunlei Yu, Keyun Ren, Kunying Nie, Jinmiao Ma, Xuezhen Xu, Qingyang Li, Qingqing Yang
{"title":"Dual-mode immunochromatographic test strips based on aggregation-induced luminescence nanoprobes for the detection of ZEN in corn.","authors":"Haitao Xu, Chunlei Yu, Keyun Ren, Kunying Nie, Jinmiao Ma, Xuezhen Xu, Qingyang Li, Qingqing Yang","doi":"10.1039/d5ay00079c","DOIUrl":"https://doi.org/10.1039/d5ay00079c","url":null,"abstract":"<p><p>Zearalenone (ZEN) is a secondary metabolite produced by various Fusarium species. Its widespread contamination has raised deep concern globally. Also, ZEN is reproductively toxic, hepatotoxic and carcinogenic. Rapid detection methods for ZEN mainly suffer from the aggregation-caused quenching (ACQ) effect of fluorescent materials. Therefore, there is an urgent need to develop new methods for detecting ZEN. On this basis, we synthesised AIE polymer microspheres (AIEPMs) using aggregation-induced emission (AIE) dyes. We coupled these microspheres with antibodies to prepare immunoprobes to establish immunochromatographic test strips with a colorimetric/fluorescent dual mode. Under optimal parameters, the visual limit of detection (vLOD) for the colorimetric mode was 0.625 ng mL<sup>-1</sup>. In comparison, the quantitative limit of detection (qLOD) for the fluorescent mode was as low as 0.039 ng mL<sup>-1</sup>. The average recoveries ranged from 97.60% to 102.46%, and the coefficients of variation (CV) ranged from 2.10% to 10.70%. These data demonstrate the excellent reproducibility and reliability of the established method. The researchers have successfully applied the method for accurate sample detection. The method demonstrated the great potential of AIEPMs-ICA as a colorimetric/fluorescent dual-mode test strip for the rapid detection of ZEN.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vellaisamy Indirakumari, Dharmalingam Sakthilatha, Kumarasamy Jayakumar, Habibulla Imran, Sooman Lim and Mohammad Rashid Khan
{"title":"Advanced detection of bisphenol A in plastic water bottles using liquid–liquid phase extraction and LC-MS","authors":"Vellaisamy Indirakumari, Dharmalingam Sakthilatha, Kumarasamy Jayakumar, Habibulla Imran, Sooman Lim and Mohammad Rashid Khan","doi":"10.1039/D4AY02094D","DOIUrl":"10.1039/D4AY02094D","url":null,"abstract":"<p >The release of endocrine-disrupting chemicals (EDCs) can harm humans and wildlife. It is therefore important to monitor bisphenol A (BPA) consumption, an endocrine disruptor commonly found in water from plastic products, and detect BPA at low concentrations for accurate health risk assessments. We present a method for estimating BPA levels in plastic-bottled products that is highly sensitive, precise, and effective. BPA analysis was performed using advanced liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS), with multiple reaction monitoring (MRM) on a state-of-the-art Orbitrap mass spectrometry system using negative ionization techniques. To assess the quality of Indian brands of water, we used LC-MS to obtain balanced hydrophilic–lipophilic extracts. Brand water samples showed efficiently separated BPA in 2.35 minutes, with other sources typically taking between 5 and 8 minutes. BPA concentrations, measured within a range of 10 ng mL<small><sup>−1</sup></small> to 1 pg mL<small><sup>−1</sup></small> with a lower detection limit (LOD) of 0.037 ng mL<small><sup>−1</sup></small>, were characterized by dynamic linear ranges and corresponding linear equations for each compound. We also evaluated the reproducibility and sensitivity of the detection of BPA in different water samples, including mineral, river, and tap water, with low levels of BPA found in Indian river water (below 4.54 ng mL<small><sup>−1</sup></small>). Thus, this study explored alternatives to solid phase extraction (SPE) for screening BPA analogs in water samples, and real samples from an Indian supermarket revealed BPA in plastic bottles at concentrations comparable to those described in Europe, the United States, Korea, Japan, and China.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 10","pages":" 2355-2363"},"PeriodicalIF":2.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of a novel label-free NIR aptasensor based on triphenylmethane dyes for rapid and sensitive detection of copper ions†","authors":"Junhao Hu, Xinxin Li, Teck-Peng Loh and Lingli Bu","doi":"10.1039/D5AY00156K","DOIUrl":"10.1039/D5AY00156K","url":null,"abstract":"<p >Heavy metal pollution, particularly from copper ions (Cu<small><sup>2+</sup></small>), poses a significant threat to both the ecological environment and human health. However, traditional copper ion analysis techniques are often hindered by the need for expensive equipment, labor-intensive sample preparation and skilled operation, which limits their effectiveness for field and real-time applications. In this work, we report a novel near-infrared aptamer sensor (NIRApt) that originates from the binding reaction between the DNA aptamer Apt<small><sub>Cu</sub></small> and the fluorescent small molecule crystal violet (CV), enabling rapid detection of Cu<small><sup>2+</sup></small> through the competitive effect of Cu<small><sup>2+</sup></small> with Apt<small><sub>Cu</sub></small>. This sensor shows a significant enhancement in NIR fluorescence after aptamer binding. NIRApt exhibits superior performance, requiring only three core components to achieve a fast response time and operational simplicity in less than a minute. The sensor shows high sensitivity with a detection limit as low as 61 nM, making it suitable for the detection of trace amounts of Cu<small><sup>2+</sup></small> in diverse samples. The efficacy of NIRApt has been validated through successful applications in real water samples, highlighting its promising potential for environmental monitoring.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 12","pages":" 2536-2540"},"PeriodicalIF":2.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Meiling Zhu, Weiran Song, Xuan Tang and Xiangzeng Kong
{"title":"Quantitative analysis of creatine monohydrate using near-infrared spectroscopy and hyperspectral imaging combined with multi-model fusion and data fusion strategies","authors":"Meiling Zhu, Weiran Song, Xuan Tang and Xiangzeng Kong","doi":"10.1039/D5AY00072F","DOIUrl":"10.1039/D5AY00072F","url":null,"abstract":"<p >Creatine monohydrate is an important sports nutrition supplement that enhances energy and promotes muscle growth. Recent concerns about the quality and authenticity of creatine monohydrate have highlighted the urgent need for rapid and cost-effective assessment methods. This study presents a new approach for assessing the quality of creatine monohydrate using spectroscopy combined with machine learning. Spectral data of creatine monohydrate samples from 15 brands are acquired using portable near-infrared (NIR) spectroscopy and benchtop hyperspectral imaging (HSI). Machine learning methods are employed to extract high-level features from the spectral data and model the relationship between the data and creatine concentrations. The root mean square error (RMSE) for models based on NIR data ranges from 0.258 to 0.291, whereas those derived from HSI data vary between 0.468 and 0.576. To improve the accuracy and reliability of spectral data analysis, multi-model fusion and data fusion strategies are used to integrate the outputs of different models and data from different sources, respectively. By combining NIR-HSI data fusion with multi-model fusion, the lowest RMSE for creatine quantification is reduced to 0.18. These results demonstrate that spectroscopic techniques coupled with machine learning can provide a rapid and cost-effective solution for assessing the quality and authenticity of creatine monohydrate.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 11","pages":" 2409-2416"},"PeriodicalIF":2.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ana C. O. Neves, Maria Paraskevaidi, Pierre Martin-Hirsch and Kássio M. G. de Lima
{"title":"Evaluating the effectiveness of whole blood plasma versus protein precipitates in ovarian cancer detection through infrared spectroscopy","authors":"Ana C. O. Neves, Maria Paraskevaidi, Pierre Martin-Hirsch and Kássio M. G. de Lima","doi":"10.1039/D4AY02321H","DOIUrl":"10.1039/D4AY02321H","url":null,"abstract":"<p >Early diagnosis of ovarian cancer remains challenging due to the absence of effective screening tests. The success of treatment and 5 year survival rates are significantly reliant on identifying the disease at a non-advanced stage, which highlights the urgent need for novel early detection and diagnostic approaches. Blood-based spectroscopic techniques, combined with chemometrics, have the potential to be used as tools for screening and diagnostic purposes in this context. In this study, we utilised attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy to analyse blood plasma samples from benign (<em>n</em> = 15) and ovarian cancer (<em>n</em> = 15) cases. We conducted multivariate discrimination models to compare the results in terms of sensitivity, specificity, and diagnostic accuracy when using either plasmatic protein precipitates or whole plasma to distinguish between benign and ovarian cancer. Notably, diagnostic accuracy values of 96% (sensitivity and specificity of 96%) and 92% (sensitivity and specificity of 88% and 96%, respectively) were achieved for the protein precipitates and whole plasma datasets respectively using genetic algorithms with linear and quadratic discriminant analysis. Furthermore, this methodology demonstrated its capability to categorise samples within the ovarian cancer class, distinguishing between early stage (FIGO I) and advanced stage (FIGO II–III), with excellent accuracy exceeding 97% for protein precipitate dataset. These findings highlight the utilisation of a specific class of biomolecules in a proteomic-like approach based on infrared spectroscopy and chemometrics for detecting ovarian cancer using blood plasma samples.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 11","pages":" 2477-2486"},"PeriodicalIF":2.7,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}