Analytical Methods最新文献

{"title":"Real-time smartphone-based multi-parameter detection of nitrite, ammonia nitrogen, and total phosphorus in water†","authors":"Hao-Yu Zhang, Yan Zhang, Dengni Lai, Li-Qun Chen, Le-Qian Zhou, Chang-le Tao, Zhou Fang, Rui-Rui Zhu, Wen-Qi Long, Jun-Wu Liu, Ying-Chun Fang, Jia Zhao, Zhi-Bin Wu, Lin Luo and Yuan Yang","doi":"10.1039/D5AY00251F","DOIUrl":"10.1039/D5AY00251F","url":null,"abstract":"<p >Elevated levels of ammonia nitrogen and phosphorus trigger eutrophication, spurring algal blooms, depleting oxygen, and thereby degrading water quality and killing fish. Nitrites, toxic to humans and animals, contaminate water sources when present in excess, making the water unfit for drinking and making their removal <em>via</em> standard treatments challenging. Together, these factors severely endanger aquatic life, human health, and water safety. Thus, developing on-site quantitative methods for the determination of nitrite, ammonia nitrogen, and total phosphorus in environmental waters is of great significance for environmental monitoring agencies. This study introduces a fast, cost-effective, and flexible on-site testing method that leverages a smartphone and a self-designed 3D-printed portable device to detect nitrite, ammonia nitrogen, and total phosphorus in environmental water. This constitutes an on-site analysis approach as it involves portable equipment deployed at the field location, rather than <em>in situ</em> sensing that directly probes samples within their native environment without collection. The method utilizes the smartphone's color recognition feature and an adaptive deconvolution algorithm that allows for the simultaneous detection of these pollutants using a six-channel device under optimal conditions. The detection limits for nitrite, ammonia nitrogen and total phosphorus were 0.013, 0.14 and 0.034 mg L<small>⁻¹</small>, respectively. Furthermore, the linear ranges for nitrite, ammonia nitrogen and total phosphorus were 0.02–0.86, 0.18–2.25 and 0.04–1.65 mg L<small>⁻¹</small>, respectively, and their recoveries were in ranges of 90.2 ± 1.8–108.4 ± 6.8%, 84.0 ± 7.4–107.3 ± 5.5%, and 93.8 ± 7.5–107.6 ± 2.7%, respectively. This method offers a quick, precise, and sensitive alternative to traditional analysis, reducing analysis time, minimizing sample contamination and errors, and thus providing a convenient and efficient solution for the on-site environmental monitoring of ammonia nitrogen, nitrite, and total phosphorus, suitable for rapid water quality detection.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 27","pages":" 5683-5696"},"PeriodicalIF":2.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outstanding Reviewers for Analytical Methods in 2024","authors":"","doi":"10.1039/D5AY90071A","DOIUrl":"10.1039/D5AY90071A","url":null,"abstract":"<p >We would like to take this opportunity to thank all of <em>Analytical Methods</em>’ reviewers for helping to preserve quality and integrity in chemical science literature. We would also like to highlight the Outstanding Reviewers for <em>Analytical Methods</em> in 2024.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 27","pages":" 5545-5545"},"PeriodicalIF":2.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting liposomes and an accelerated CRISPR system for selective imaging of miR-21 in cells†","authors":"Wenyue Zhang, Mengting Wu, Qingqing Zhang, Xibin Chen and Yingshu Guo","doi":"10.1039/D5AY00619H","DOIUrl":"10.1039/D5AY00619H","url":null,"abstract":"<p >This study demonstrates a liposome-targeted and accelerated CRISPR strategy for specific response of miR-21 within MCF-7 cells. Targeting was achieved with the help of modification of the MUC1 aptamer. MnO<small>₂</small> NS could be used as both a delivery vehicle and an accelerator for the CRISPR system, and finally to achieve spatially selective imaging of miR-21.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 28","pages":" 5831-5835"},"PeriodicalIF":2.7,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A polypyrrole-coated GCE sensor for sensitive detection of 5-fluorouracil via molecular imprinting†","authors":"Manjeet Harijan, Akriti Srivastava and Meenakshi Singh","doi":"10.1039/D5AY00345H","DOIUrl":"10.1039/D5AY00345H","url":null,"abstract":"<p >Cytotoxic drug 5-fluorouracil (5-FU) is a fluorine derivative of uracil; it is one of the most significant medications used to treat cancers of the stomach, breast, colon, pancreas, and cervical regions. Here, a reliable, rapid, highly sensitive and selective method is proposed for determining 5-FU in real samples. In this study, a molecularly imprinted polymer (MIP) based electrochemical sensor is designed for the sensitive and selective determination of 5-FU. The MIP was developed by the electropolymerization of a pyrrole thin film around template molecules (5-FU) on a glassy carbon electrode (GCE). The sensor was characterized after each stage of fabrication using cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS). The MIP sensor exhibited a wide linear range for determining 5-FU from 2–42 μM. The developed sensor achieved a limit of detection (LOD) and limit of quantification (LOQ) of 0.605 μM and 1.834 μM, respectively. The applicability of the proposed sensor was examined for 5-FU determination in real samples. The MIP sensor exhibited excellent selectivity, repeatability, stability, and commercialization potential for 5-FU detection. Furthermore, the proposed method offers significant advantages over existing electrochemical techniques for 5-FU detection. This method provides single-step preparation alongside simple template molecule removal by cyclic voltammetry scans and does not need any extracting solvents.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 28","pages":" 5961-5972"},"PeriodicalIF":2.7,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A lysosome targetable and acidic pH-activated fluorescent probe for two-photon imaging of hydrogen sulfide in live cells and animals†","authors":"YuJia Fu, XiaoFeng Guo and Hong Wang","doi":"10.1039/D5AY00757G","DOIUrl":"10.1039/D5AY00757G","url":null,"abstract":"<p >The design strategy of targeted fluorescent probes usually involves modifying the probes with organelle-targeting groups, which then guide the probes to enter specific organelles by the traction of the targeting groups. However, the targeting ability of subcellular localization groups is not perfect, and the target analytes are not specifically distributed within the cells. Therefore, designing novel molecular fluorescent probes for highly specific and selective detection of target analytes within specific organelles is an important research direction that deserves attention. Although multiple high-efficiency lysosomal localization fluorescent probes for H<small>₂</small>S have been developed, the pH range for the fluorescence response of these probes to H<small>₂</small>S does not match the specific acidic range of the lysosome very well. In this work, by analyzing the relationship between the structures of the pH-responsive groups and the response range, excellent acidic pH-activated lysosome-localized fluorescent probes Lyso-NP-DS and Lyso-NP-NBD with a better performance have been developed. After the reaction with H<small>₂</small>S, the fluorescence activation pH ranges of their derivatives were between 2.5 and 5.5, 3.0 and 5.5, respectively. However, this could not only solve the problem of insufficient matching between the pH activation range for the fluorescence response to H<small>₂</small>S and the specific acidic range within the lysosome, but it also shows the characteristics of two-photon excitation and high sensitivity. This effectively avoids the background interference and extracellular fluorescence interference in the lysosome, and an accurate and efficient detection of H<small>₂</small>S within the lysosome is achieved.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 26","pages":" 5372-5382"},"PeriodicalIF":2.7,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144482556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A hybridization chain reaction-based electrochemical sensor for rapid detection of respiratory pathogens†","authors":"Yuting Shan, Dongmei Zhang, Yao Liu, Yijie Wang, Jiufa Zhang, Cuiping Ma, Yang Li and Chao Shi","doi":"10.1039/D5AY00418G","DOIUrl":"10.1039/D5AY00418G","url":null,"abstract":"<p >Respiratory viral infections continue to pose a significant challenge to global public health. Electrochemical nucleic acid sensors, with their high sensitivity and ease of miniaturization, demonstrate great diagnostic potential. However, the development of high-performance sensors requires comprehensive consideration of factors such as capture probe density, steric hindrance effects, and electrostatic repulsion, which pose significant challenges for the direct detection of long-sequence nucleic acids and limit the application of electrochemical sensors in pathogen diagnosis. This work reports a novel sensor platform based on a concatenated DNA circuit and modified electrodes to achieve efficient and rapid detection of long-sequence nucleic acids. First, the pathogen genomic target sequence replaces the Trigger strand (Ts) through a toehold displacement (TD) reaction. After this, Ts initiates the HCR and the two biotin-modified hairpins hybridize to generate a long double-stranded product. Crowding agents were introduced into the system to enhance the hybridization efficiency of the toehold displacement-mediated hybridization chain reaction (TDHCR) by increasing the local nucleic acid concentration and reducing the free water content in the system, resulting in a significant reduction in reaction time from 120 min to 30 min. Subsequently, the biotin labeled on the TDHCR products rapidly binds to streptavidin immobilized on the electrode surface, enabling efficient and rapid product immobilization. This strategy overcomes the common limitation of low nucleic acid hybridization efficiency at the solid–liquid interface. Finally, the HRP-catalyzed redox reaction between 3,3′,5,5′-tetramethylbenzidine (TMB) and H<small>₂</small>O<small>₂</small> converts the DNA hybridization event into a measurable electrochemical signal, generating a significant current response. The entire detection process can be completed in less than 50 min, with a detection limit as low as 4.876 fM. This platform demonstrates great potential for clinical pathogen detection and is well-suited for integration into microfluidic devices.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 26","pages":" 5498-5507"},"PeriodicalIF":2.7,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144482555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemiluminescence video assisted by chemometric modeling for forensic identification of blood at crime scenes†","authors":"Thomas F. F. T. dos Santos, José R. S. Júnior, Licarion Pinto, Tadeu Morais Cruz, Jose Ailton M. Nascimento, Severino Carlos B. Oliveira and Vagner Bezerra dos Santos","doi":"10.1039/D5AY00633C","DOIUrl":"10.1039/D5AY00633C","url":null,"abstract":"<p >The development of advanced chemiluminescent compounds and hematology methodologies has significant implications for forensic science, particularly for the detection of evidential residues at crime scenes. This study introduces a novel chemiluminescent (CL) method that utilizes a smartphone to produce digital videos of the chemiluminescent reaction between the luminol (5-amino-2,3-dihydrophthalazine-1,4-dione) reagent and blood. This innovative approach significantly reduces reagent consumption by 6 times, requiring less than 1 mL/0.01 g of sample/chemicals, which agrees with green chemistry principles. Blood samples used in this study were sourced from bovine liver and human subjects and were collected by the official forensic police at crime scenes. All samples were subsequently discarded by the criminal police. Frames from a 3-minutes video were processed using ImageJ software and the Color Grab app to generate RGB, HSV, and CMYK pattern recognition, combined with chemometric modeling. This enabled the differentiation of samples based on positive and negative patterns, effectively preventing false results. The pattern recognition models developed were able to distinguish bovine from human blood, even after dilution, which simulated attempts to hide traces at crime scenes through washing. The method demonstrated an accuracy of 90.30% with only four prediction errors and presented 100% sensitivity and specificity for the cotton + ceramics class, with 77.78% sensitivity and 93.10% specificity for both the wood and glass classes. Additionally, it was possible to estimate the age of the samples with a precision of 3.6 days. These results were obtained using a new data fusion strategy that facilitated the modeling of digital videos as a combination of frames to enhance model sensitivity and selectivity without increasing model complexity. These results indicate that the developed method is accurate, sensitive, and rapid. Supported by these results, this method represents a significant advancement in forensic science, offering a practical and efficient solution for crime scene investigations.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 28","pages":" 5836-5848"},"PeriodicalIF":2.7,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144598938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective detection of tyrosine kinase inhibitor erdafitinib using nitrogen-doped carbon dots synthesized at room temperature†","authors":"Mohamed N. Goda, Laila S. Alqarni, Hossieny Ibrahim, Al-Montaser Bellah H. Ali and Mohamed M. El-Wekil","doi":"10.1039/D5AY00833F","DOIUrl":"10.1039/D5AY00833F","url":null,"abstract":"<p >Erdafitinib (ERDF), despite its known adverse effects, remains a key chemotherapeutic agent for metastatic urothelial carcinoma, requiring reliable monitoring in biological matrices to ensure safe and effective treatment. In this study, we developed a cost-effective, energy-efficient method for synthesizing highly fluorescent nitrogen-doped carbon dots (NCDs) using simple precursors. The resulting NCDs displayed excellent photostability and a high fluorescence quantum yield of 41.87%. ERDF induced a concentration-dependent quenching of the NCDs' fluorescence at 520 nm, attributed to the inner filter effect, with a low detection limit of 0.013 μM (S/N = 3). The NCDs demonstrated high selectivity toward ERDF with minimal interference from coexisting substances. Recovery rates in spiked human serum and urine ranged from 97.8% to 104.4%, validating the method's practical applicability. The relative standard deviations (RSDs) remained below 3.72%, confirming the precision and reproducibility of the NCD-based sensing platform.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 28","pages":" 5929-5938"},"PeriodicalIF":2.7,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mediating in situ-generated fluorescence for Au nanocluster-based multi-color ratiometric detection of alkaline phosphatase†","authors":"Chao-Zhe Zhu, Shuo Tian, Hua-Nong Ting, Mengke Wang and Yufeng Wang","doi":"10.1039/D5AY00835B","DOIUrl":"10.1039/D5AY00835B","url":null,"abstract":"<p >The portable, rapid, and accurate detection methods for alkaline phosphatase (ALP) can significantly improve the efficiency and accuracy of medical testing, especially in early disease screening. Herein, a fluorescent detection was constructed for ALP based on the ALP-mediated <em>in situ</em>-generated fluorescent product for the first time, which employed Au nanoclusters (AuNCs) and ALP-mediated <em>in situ</em> generated fluorescent products as probes by applying 4-methylumbelliferyl phosphate (MeUP) as a substrate. ALP can catalyze the hydrolysis of MeUP to generate 4-methylumbelliferon (MeU), which exhibits blue emission centered at 440 nm. Meanwhile, the AuNCs kept their red fluorescence at 630 nm as a reference signal. Accordingly, a ratiometric fluorescent sensing of ALP was proposed with a consecutive fluorescent color change with a detection limit of 0.0025 U L<small>⁻¹</small>. The pronounced fluorescence signal variation served as the impetus for the development of a facile, cost-effective portable hydrogel kit, which facilitates equipment-free, visual, and quantitative analysis of ALP activity. Additionally, this system was employed for detecting ALP inhibitors using sodium metavanadate (Na<small>₃</small>VO<small>₄</small>) as a model. Notably, this sensing method and hydrogel kit were applied for ALP detection in human serum samples, yielding promising results.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 28","pages":" 5920-5928"},"PeriodicalIF":2.7,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasensitive detection of deoxynivalenol by mass spectrometric immunoassay with cascade signal amplification†","authors":"Hanxu Ji, JingJing Xu, Yufeng Li, Xiaoxiao Wu, Diyao Jiang, Xiuming Mei, Miao Yang, Yuwei Qiang, Jungui Zhou and Chi Zhang","doi":"10.1039/D5AY00724K","DOIUrl":"10.1039/D5AY00724K","url":null,"abstract":"<p >We developed a novel mass spectrometry immunoassay for the ultrasensitive and selective detection of deoxynivalenol by introducing nucleic-acid-amplification techniques. By selecting thymine as a mass tag, the primer DNA modified on the antibody could serve dual functions as both a signal probe and an initiator for the cascade amplification reaction.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 29","pages":" 5993-5996"},"PeriodicalIF":2.7,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}