Analytical Methods最新文献_第6页

Development of chemiluminescence enzyme immunoassay (CLEIA) for the determination of chlorogenic acid in pharmaceutical products†

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-03-12 DOI: 10.1039/D5AY00324E

Long Xu, Yuzhu Xu, Jingdi Niu, Feiyue Xu, Jian Qu, Yongsheng Qiao and Wanwan Wang

{"title":"Development of chemiluminescence enzyme immunoassay (CLEIA) for the determination of chlorogenic acid in pharmaceutical products†","authors":"Long Xu, Yuzhu Xu, Jingdi Niu, Feiyue Xu, Jian Qu, Yongsheng Qiao and Wanwan Wang","doi":"10.1039/D5AY00324E","DOIUrl":"10.1039/D5AY00324E","url":null,"abstract":"Chlorogenic acid (CGA) is an important and abundant bioactive compound, exhibiting various pharmacological properties including antifungal, anti-inflammatory, antioxidant, antiviral, neuroprotective and antispasmodic activities. CGA is available in many types of pharmaceutical products in the form of tablets, capsules, and injections derived from plants. The CGA content is typically regarded as an important indicator for the quality control of these pharmaceutical products. Therefore, it is particularly important to develop a reliable and accurate method for the determination of CGA. In this study, CGA was coupled to bovine serum albumin (BSA) and ovalbumin (OVA) using an active ester method to synthesise artificial antigens. A sensitive and specific monoclonal antibody (mAb) against CGA was obtained. To analyse CGA efficiently, an indirect competitive chemiluminescent enzyme immunoassay (ic-CLEIA) was developed on the basis of the generated mAb. Under optimal conditions, the limit of detection (LOD) and half-maximal inhibitory concentration (IC50) were 1.76 ng mL−1 and 18 ng mL−1, respectively. The linear range was from 2.5–100 ng mL−1, with an R2 value of 0.9963. The recoveries of CGA in spiked pharmaceutical products ranged from 77.2% to 118.3%, with coefficients of variation (CVs) ranging from 1.7% to 11.2%. The samples were validated by high-performance liquid chromatography (HPLC) coupled to a UV detector at 327 nm. The ic-CLEIA results showed a high correlation coefficient of 0.9718 when compared with those obtained by HPLC, demonstrating that the proposed ic-CLEIA would be a credible method for the quantification of CGA in pharmaceutical products.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 13","pages":" 2733-2739"},"PeriodicalIF":2.7,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploration of a modified pretreatment process coupled with GC-MS/MS for determination of 18 phthalates in edible oils†

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-03-12 DOI: 10.1039/D4AY02049A

Pei-Jin Wang, Long-Fei Lin, Yu-Jie Wu, Tian-Rong Guo, Quan-Wei Xiao and Wen-Lin Wu

{"title":"Exploration of a modified pretreatment process coupled with GC-MS/MS for determination of 18 phthalates in edible oils†","authors":"Pei-Jin Wang, Long-Fei Lin, Yu-Jie Wu, Tian-Rong Guo, Quan-Wei Xiao and Wen-Lin Wu","doi":"10.1039/D4AY02049A","DOIUrl":"10.1039/D4AY02049A","url":null,"abstract":"Phthalates (PAEs), which are pollutants that most easily migrate from plastic packages to edible oils, have received increasing attention. In this work, a sample preconditioning method was proposed and explored, involving a single-step extraction using a methanol and ethyl hexanoate mixed solvent, followed by clean-up with a mixed absorbent of graphitic carbon nitride (g-C3N4) and N-propyl ethylenediamine (PSA), for the determination of 18 PAEs in oil samples through GC-MS/MS analysis. The triazine ring structure and conjugated aromatic heterocyclic accumulation structure of g-C3N4 and the amino-rich structure of PSA can provide hydrogen bonds, conjugated π–π interactions, and hydrophobic effects for the removal of interferences such as organic acids, aliphatic acids, and pigments. To achieve optimal analytical performance, key extraction and purification parameters that may affect the pretreatment efficiency—such as the extraction solution, its volume ratio, extraction temperature, extraction time, and the amount of the clean-up absorbent—were systematically explored and optimized. When applied to edible oil analysis, an excellent linear range from 0.2 to 8.0 mg kg−1 with a preferable coefficient of determination (R2 > 0.9989) and satisfactory sensitivity (LOD and LOQ ranging from 0.0002 mg kg−1 to 0.08 mg kg−1 and 0.0004 mg kg−1 to 0.2 mg kg−1, respectively) were obtained. Moreover, a relatively satisfactory recovery varying from 71.4% to 107.9% and the corresponding repeatability ranging from 0.3% to 6.8% were acquired. Furthermore, this work offers a new idea for exploring the application of novel nanocomposites in the pretreatment process.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 12","pages":" 2607-2615"},"PeriodicalIF":2.7,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzyme cycling method using hypoxanthine-guanine phosphoribosyltransferase: a highly sensitive assay for pyrophosphate†

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-03-11 DOI: 10.1039/D4AY01692K

Shigeru Ueda, Yoshiaki Yasutake, Tatsuya Hirata, Takehiko Sahara and Shin-ichi Sakasegawa

引用次数: 0

Raman spectroscopy for forensic pen ink investigation: a review

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-03-11 DOI: 10.1039/D4AY01951B

Rosane R. Souza, Lucas M. Duarte and Glaucio B. Ferreira

引用次数: 0

Indirect ELISA-based detection of histatin 3 and cystatin D for the forensic identification of human saliva†

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-03-11 DOI: 10.1039/D4AY01995D

Jun Ohta, Mana Nagata, Nanaka Noda, Saki Minegishi, Hisako Saitoh and Koichi Sakurada

{"title":"Indirect ELISA-based detection of histatin 3 and cystatin D for the forensic identification of human saliva†","authors":"Jun Ohta, Mana Nagata, Nanaka Noda, Saki Minegishi, Hisako Saitoh and Koichi Sakurada","doi":"10.1039/D4AY01995D","DOIUrl":"10.1039/D4AY01995D","url":null,"abstract":"Analyzing forensically relevant body fluids contributes to proving criminal acts, and saliva is often left on the scene, especially in sexual assault cases. Currently, saliva is presumptively identified using its salivary α-amylase activity as an indicator. However, the specificity of saliva presumptive tests is low, and therefore, they cannot confidently prove the presence of saliva. This study aimed to develop and validate an indirect ELISA-based saliva confirmatory test using the novel human salivary protein markers histatin 3 and cystatin D. Histatin 3 and cystatin D are known to be uniquely expressed in human salivary glands, and the molecular evolution of both genes occurred after the divergence of hominoids; thus, the high specificity of human saliva for both markers was expected. As a result of the validation experiments, both histatin 3 and cystatin D markers were able to distinguish human saliva from other human body fluids and animal saliva using appropriate thresholds of the quantitative absorbance values. Our results indicate that both histatin 3 and cystatin D markers are human saliva-specific; thus, we consider that this method will be useful and practical in identifying human saliva.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 12","pages":" 2591-2599"},"PeriodicalIF":2.7,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid quantification of pullulan in fermentation broth using UV-visible spectroscopy and partial least squares regression†

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-03-10 DOI: 10.1039/D5AY00034C

Nageswar Sahu, Biswanath Mahanty and Dibyajyoti Haldar

{"title":"Rapid quantification of pullulan in fermentation broth using UV-visible spectroscopy and partial least squares regression†","authors":"Nageswar Sahu, Biswanath Mahanty and Dibyajyoti Haldar","doi":"10.1039/D5AY00034C","DOIUrl":"10.1039/D5AY00034C","url":null,"abstract":"Quantification of exopolysaccharide (EPS) production in fermentation broth requires solvent precipitation of the polymer, followed by acid or enzymatic hydrolysis, and colorimetric or chromatographic analysis. This lengthy multistep sample preparation and analysis is a major bottleneck in bioprocess monitoring. The development of a nondestructive analytical method requiring minimal sample preparation is warranted. In this study, partial least squares (PLS) regression models were developed to quantify pullulan in cell-free supernatant (PCS) and precipitated pullulan redissolved in distilled water (PDW) from spectral data (204–400 nm). Genetic algorithm, particle swarm optimization, competitive adaptive reweighted sampling, and adaptive bottom-up space exploration strategies were employed to select optimal spectral regions. The full-spectrum model on the PCS (5 latent variables, RMSECV: 0.020 g l−1, RCV2: 0.997) outperformed the PDW (3 latent variables, RCV2: 0.990). Adaptive bottom-up space exploration achieved the lowest RMSECV (0.009 g l−1 for the PCS, 0.027 g l−1 for the PDW), retaining just 16 and 21 spectral variables, respectively. The residual predictive deviation (RPD) for all PLS model variants remains satisfactory (>6.559). The method's limit of detection (0.021 g l−1) was suitable for quantifying pullulan in fermentation broth. The proposed method can be extended to other structurally similar biopolymers where PLS-based soft sensor integration would enable real-time monitoring and bioprocess control.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 14","pages":" 2841-2849"},"PeriodicalIF":2.7,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143661620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensitive detection of Golgi protein 73 by magnetic separation combined with a dual nanozyme MOF-818 and magnetic nucleus@bifunctional shell (Fe3O4@PB-Au) cascade reaction catalytic amplification strategy†

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-03-10 DOI: 10.1039/D4AY02256D

Wei Li, Xin Hu, Anyi Chen, Rongxin Wu and Chaorui Li

{"title":"Sensitive detection of Golgi protein 73 by magnetic separation combined with a dual nanozyme MOF-818 and magnetic nucleus@bifunctional shell (Fe3O4@PB-Au) cascade reaction catalytic amplification strategy†","authors":"Wei Li, Xin Hu, Anyi Chen, Rongxin Wu and Chaorui Li","doi":"10.1039/D4AY02256D","DOIUrl":"10.1039/D4AY02256D","url":null,"abstract":"Metal–organic framework (MOF-818) and nanocomposite (Fe3O4@PB-Au) dual nanozymes for enhanced cascade signal amplification were designed. MOF-818 has excellent catechol oxidase mimetic activity and catalyzes the production of color and in situ generation of hydrogen peroxide from the substrate 3,5-di-tert-butylcatechol (3,5-DTBC). Subsequently, Fe3O4@PB-Au with peroxidase-like activity catalyzes the generation of reactive oxygen species from hydrogen peroxide, which oxidizes 3,3′,5,5′-tetramethylbenzidine (TMB) to generate the oxidized state of oxTMB, resulting in a signal-enhancing effect. The prepared dual nanozymes can be combined with aptamers with specific recognition ability, thus developing a colorimetric aptamer sensor with high sensitivity and selectivity for detecting Golgi protein 73, which provides a feasible assay for clinical detection of GP73 protein. Detection of GP73 was accomplished by measuring the UV absorption peak at 415 nm. Under the optimal conditions, the concentration of GP73 was linearly correlated with the absorbance in the range of 10.0–100.0 ng mL−1 with a detection limit of 1.83 ng mL−1. The proposed colorimetric biosensor was successfully applied to the determination of GP73 in spiked human serum samples with recoveries of 96.15–100.95% and RSDs of 1.52–6.85%, which demonstrated the great potential of the highly sensitive GP73 assay in clinical detection.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 12","pages":" 2567-2576"},"PeriodicalIF":2.7,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gaining insights into nutrient and metal element distributions in radix Angelicae sinensis using mass spectrometry†

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-03-10 DOI: 10.1039/D5AY00106D

Shiyu He, Boyan Li, Lan Wang, Zihan Wang, Jin Zhang, Yun Hu and Yali Wang

引用次数: 0

Structurally modified carbon nanotubes as twisted fibers towards electrochemical detection of environmentally hazardous cartap†

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-03-10 DOI: 10.1039/D5AY00087D

Gul Tasam, Sheza Muqaddas, Abid Ali, Maryam Kaleem, Farah Kanwal, Munawar Iqbal, Norah Alwadai and Arif Nazir

{"title":"Structurally modified carbon nanotubes as twisted fibers towards electrochemical detection of environmentally hazardous cartap†","authors":"Gul Tasam, Sheza Muqaddas, Abid Ali, Maryam Kaleem, Farah Kanwal, Munawar Iqbal, Norah Alwadai and Arif Nazir","doi":"10.1039/D5AY00087D","DOIUrl":"10.1039/D5AY00087D","url":null,"abstract":"Pesticides such as cartap play a crucial role in agriculture, as they are extensively used in controlling pests in crops like sugarcane, rice and vegetables. However, their excessive usage poses major threats to human health, causing neurotoxicity and respiratory disorders. In addition, they harm aquatic ecosystems by disrupting biodiversity. Consequently, effective detection and remediation are essential to minimize their adverse effects on human health and the ecosystem. For this purpose, an electrochemical method was used for the simultaneous detection and degradation of the under-reported insecticide cartap. In this work, vertically aligned carbon nanotube arrays were grown and converted into fibers via simple mechanical spinning and used as a flexible electrode material for cartap electro-oxidation. The fabricated electrode exhibited excellent performance towards sensing and remediation without any surface modification. The fiber-based electrode showed a wide linear range, a low detection limit (LOD) of 0.575 mM, high sensitivity (7.98 μA cm−2 mM−1), reproducibility, and excellent electrocatalytic activity. The multi-functionality of this electrode was attributed to its unique properties like flexibility, compatibility with various media (aqueous and organic), cost-effectiveness, and seamless integration with membranes, making these fibers promising candidates for on-site facile detection and long-term degradation of cartap.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 12","pages":" 2550-2558"},"PeriodicalIF":2.7,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conventional versus AI-based spectral data processing and classification approaches to enhance LIBS's analytical performance†

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-03-07 DOI: 10.1039/D5AY00027K

Zakaria E. Ahmed, Rania M. Abdelazeem, Mahmoud Abdelhamid, Zienab Abdel-Salam and Mohamed Abdel-Harith

{"title":"Conventional versus AI-based spectral data processing and classification approaches to enhance LIBS's analytical performance†","authors":"Zakaria E. Ahmed, Rania M. Abdelazeem, Mahmoud Abdelhamid, Zienab Abdel-Salam and Mohamed Abdel-Harith","doi":"10.1039/D5AY00027K","DOIUrl":"10.1039/D5AY00027K","url":null,"abstract":"Laser-Induced Breakdown Spectroscopy (LIBS) combined with Artificial Intelligence (AI) offers a powerful method for analyzing and comparing spectral data. This study presents a comparative analysis of conventional and AI-developed methods for processing and interpreting LIBS data, especially in forensic applications, focusing on toner sample discrimination. We propose a novel AI-developed approach that combines normalization, interpolation, and peak detection techniques to simplify LIBS spectral analysis without user preprocessing and easily identify unique spectral features. This method was compared with conventional principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA), which are commonly used for LIBS data analysis. The AI-developed method demonstrated superior performance in discriminating between toner samples from various brands and models of printers and photocopiers. The quantitative evaluation of the performance of the AI-developed approach was performed using statistical analysis, including accuracy difference percentage, component-wise variance analysis, paired t-test, and cross-validation test. The results confirmed a significant improvement in accuracy with the AI-developed method compared to conventional approaches. This proposed work highlights the potential of AI in enhancing spectroscopic analysis for forensic applications, offering increased efficiency and accuracy in sample discrimination and classification. Additionally, it accelerates the analysis of LIBS data with no need for user preprocessing.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 13","pages":" 2771-2782"},"PeriodicalIF":2.7,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0