Selective detection of tyrosine kinase inhibitor erdafitinib using nitrogen-doped carbon dots synthesized at room temperature†

IF 2.6 3区化学 Q2 CHEMISTRY, ANALYTICAL

Analytical Methods Pub Date : 2025-06-23 DOI:10.1039/D5AY00833F

Mohamed N. Goda, Laila S. Alqarni, Hossieny Ibrahim, Al-Montaser Bellah H. Ali and Mohamed M. El-Wekil

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Abstract

Erdafitinib (ERDF), despite its known adverse effects, remains a key chemotherapeutic agent for metastatic urothelial carcinoma, requiring reliable monitoring in biological matrices to ensure safe and effective treatment. In this study, we developed a cost-effective, energy-efficient method for synthesizing highly fluorescent nitrogen-doped carbon dots (NCDs) using simple precursors. The resulting NCDs displayed excellent photostability and a high fluorescence quantum yield of 41.87%. ERDF induced a concentration-dependent quenching of the NCDs' fluorescence at 520 nm, attributed to the inner filter effect, with a low detection limit of 0.013 μM (S/N = 3). The NCDs demonstrated high selectivity toward ERDF with minimal interference from coexisting substances. Recovery rates in spiked human serum and urine ranged from 97.8% to 104.4%, validating the method's practical applicability. The relative standard deviations (RSDs) remained below 3.72%, confirming the precision and reproducibility of the NCD-based sensing platform.

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室温合成氮掺杂碳点选择性检测酪氨酸激酶抑制剂埃尔达非替尼。

Erdafitinib （ERDF）尽管有已知的副作用，但仍然是转移性尿路上皮癌的关键化疗药物，需要在生物基质中进行可靠的监测，以确保安全有效的治疗。在这项研究中，我们开发了一种成本效益高，节能的方法来合成高荧光氮掺杂碳点（NCDs）使用简单的前体。所制备的NCDs具有良好的光稳定性和41.87%的荧光量子产率。ERDF诱导NCDs的荧光在520 nm处呈浓度依赖性猝灭，这是由于内部过滤效应，检测限低至0.013 μM （S/N = 3）。NCDs对ERDF具有很高的选择性，且对共存物质的干扰最小。加标后的人血清和尿液的回收率为97.8% ~ 104.4%，验证了该方法的实用性。相对标准偏差（rsd）保持在3.72%以下，证实了基于ncd的传感平台的精密度和重现性。

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