Mediating in situ-generated fluorescence for Au nanocluster-based multi-color ratiometric detection of alkaline phosphatase†

IF 2.6 3区 化学 Q2 CHEMISTRY, ANALYTICAL
Chao-Zhe Zhu, Shuo Tian, Hua-Nong Ting, Mengke Wang and Yufeng Wang
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Abstract

The portable, rapid, and accurate detection methods for alkaline phosphatase (ALP) can significantly improve the efficiency and accuracy of medical testing, especially in early disease screening. Herein, a fluorescent detection was constructed for ALP based on the ALP-mediated in situ-generated fluorescent product for the first time, which employed Au nanoclusters (AuNCs) and ALP-mediated in situ generated fluorescent products as probes by applying 4-methylumbelliferyl phosphate (MeUP) as a substrate. ALP can catalyze the hydrolysis of MeUP to generate 4-methylumbelliferon (MeU), which exhibits blue emission centered at 440 nm. Meanwhile, the AuNCs kept their red fluorescence at 630 nm as a reference signal. Accordingly, a ratiometric fluorescent sensing of ALP was proposed with a consecutive fluorescent color change with a detection limit of 0.0025 U L−1. The pronounced fluorescence signal variation served as the impetus for the development of a facile, cost-effective portable hydrogel kit, which facilitates equipment-free, visual, and quantitative analysis of ALP activity. Additionally, this system was employed for detecting ALP inhibitors using sodium metavanadate (Na3VO4) as a model. Notably, this sensing method and hydrogel kit were applied for ALP detection in human serum samples, yielding promising results.

Abstract Image

介导基于金纳米团簇的碱性磷酸酶多色比例检测的原位荧光。
便携式、快速、准确的碱性磷酸酶(ALP)检测方法可以显著提高医学检测的效率和准确性,特别是在疾病早期筛查中。本文首次以4-甲基红花酰磷酸(MeUP)为底物,以Au纳米团簇(aucs)和ALP介导的原位生成荧光产物为探针,构建了基于ALP介导的原位生成荧光产物的ALP荧光检测。ALP可以催化MeUP水解生成4- methylumbellliferon (MeU), MeU的中心波长为440 nm。同时,aunc保持630 nm的红色荧光作为参考信号。据此,提出了一种连续荧光颜色变化的ALP比例荧光检测方法,检测限为0.0025 U L-1。这种明显的荧光信号变化推动了一种简便、经济的便携式水凝胶试剂盒的开发,该试剂盒有助于对ALP活性进行无设备、可视化和定量分析。此外,该系统还被用于检测ALP抑制剂,以偏氰酸钠(Na3VO4)为模型。值得注意的是,该传感方法和水凝胶试剂盒已应用于人血清中ALP的检测,结果令人满意。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Analytical Methods
Analytical Methods CHEMISTRY, ANALYTICAL-FOOD SCIENCE & TECHNOLOGY
CiteScore
5.10
自引率
3.20%
发文量
569
审稿时长
1.8 months
期刊介绍: Early applied demonstrations of new analytical methods with clear societal impact
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