Chao-Zhe Zhu, Shuo Tian, Hua-Nong Ting, Mengke Wang and Yufeng Wang
{"title":"Mediating in situ-generated fluorescence for Au nanocluster-based multi-color ratiometric detection of alkaline phosphatase†","authors":"Chao-Zhe Zhu, Shuo Tian, Hua-Nong Ting, Mengke Wang and Yufeng Wang","doi":"10.1039/D5AY00835B","DOIUrl":null,"url":null,"abstract":"<p >The portable, rapid, and accurate detection methods for alkaline phosphatase (ALP) can significantly improve the efficiency and accuracy of medical testing, especially in early disease screening. Herein, a fluorescent detection was constructed for ALP based on the ALP-mediated <em>in situ</em>-generated fluorescent product for the first time, which employed Au nanoclusters (AuNCs) and ALP-mediated <em>in situ</em> generated fluorescent products as probes by applying 4-methylumbelliferyl phosphate (MeUP) as a substrate. ALP can catalyze the hydrolysis of MeUP to generate 4-methylumbelliferon (MeU), which exhibits blue emission centered at 440 nm. Meanwhile, the AuNCs kept their red fluorescence at 630 nm as a reference signal. Accordingly, a ratiometric fluorescent sensing of ALP was proposed with a consecutive fluorescent color change with a detection limit of 0.0025 U L<small><sup>−1</sup></small>. The pronounced fluorescence signal variation served as the impetus for the development of a facile, cost-effective portable hydrogel kit, which facilitates equipment-free, visual, and quantitative analysis of ALP activity. Additionally, this system was employed for detecting ALP inhibitors using sodium metavanadate (Na<small><sub>3</sub></small>VO<small><sub>4</sub></small>) as a model. Notably, this sensing method and hydrogel kit were applied for ALP detection in human serum samples, yielding promising results.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 28","pages":" 5920-5928"},"PeriodicalIF":2.6000,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Methods","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/ay/d5ay00835b","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
The portable, rapid, and accurate detection methods for alkaline phosphatase (ALP) can significantly improve the efficiency and accuracy of medical testing, especially in early disease screening. Herein, a fluorescent detection was constructed for ALP based on the ALP-mediated in situ-generated fluorescent product for the first time, which employed Au nanoclusters (AuNCs) and ALP-mediated in situ generated fluorescent products as probes by applying 4-methylumbelliferyl phosphate (MeUP) as a substrate. ALP can catalyze the hydrolysis of MeUP to generate 4-methylumbelliferon (MeU), which exhibits blue emission centered at 440 nm. Meanwhile, the AuNCs kept their red fluorescence at 630 nm as a reference signal. Accordingly, a ratiometric fluorescent sensing of ALP was proposed with a consecutive fluorescent color change with a detection limit of 0.0025 U L−1. The pronounced fluorescence signal variation served as the impetus for the development of a facile, cost-effective portable hydrogel kit, which facilitates equipment-free, visual, and quantitative analysis of ALP activity. Additionally, this system was employed for detecting ALP inhibitors using sodium metavanadate (Na3VO4) as a model. Notably, this sensing method and hydrogel kit were applied for ALP detection in human serum samples, yielding promising results.