Analytical Methods最新文献_第2页

Surface enhanced Raman scattering microtips for microenvironment pH determination of semi-solid preparations. 用于测定半固体制剂微环境 pH 值的表面增强拉曼散射微尖。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-10-07 DOI: 10.1039/d4ay01523a

Xiangxin Lu, Yunqing Wang, Rongchao Mei, Xiaomeng Chong, Lingxin Chen, Baoming Ning, Rongqin Zhang, Xuming Zhuang

{"title":"Surface enhanced Raman scattering microtips for microenvironment pH determination of semi-solid preparations.","authors":"Xiangxin Lu, Yunqing Wang, Rongchao Mei, Xiaomeng Chong, Lingxin Chen, Baoming Ning, Rongqin Zhang, Xuming Zhuang","doi":"10.1039/d4ay01523a","DOIUrl":"https://doi.org/10.1039/d4ay01523a","url":null,"abstract":"Semi-solid preparations such as ointments, creams, gels, and pastes are common topical dosage forms with complex compositions and microstructures. pH is a critical quality attribute for semi-solid preparations, affecting properties such as particle size distribution, drug dissociation state, and rheological behavior. Currently, traditional pH electrode methods only provide an \"average\" pH for large-volume matrices, with low spatial resolution and accuracy. Microenvironment pH detection is crucial for accurately assessing semi-solid preparations. Herein, we developed pH-sensitive surface enhanced Raman scattering (SERS) microtips to achieve localized pH detection in semi-solid preparations. SERS microtips were prepared from glass needles with a tip size of around 1 μm and gold nanoparticles (Au NPs) grown in situ on glass surfaces for SERS enhancement. 4-Mercaptopyridine was selected as a pH sensitive Raman reporter and immobilized on the Au NPs, exhibiting characteristic Raman peak shifts within the pH range of 3-10. The SERS microtips were employed to conduct highly sensitive pH measurements in localized areas of 15 commercial ointments, 8 gels, and 1 laboratory-made ointment, providing higher spatial resolution and microenvironment differentiation compared to pH meters. The SERS microtips were used to monitor pH changes over time in ointment applied to localized wounds on live mice. This work introduces a new tool for pH detection in semi-solid preparations, offering a new method to enhance the prescription process and quality assessment of complex preparations like topical semi-solid preparations.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142379517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A label-free and ultrasensitive electrochemical biosensor using hybrid polypyrrole/gold nanoelectrocatalyst mediated signal amplification for the detection of miRNA-21. 利用混合聚吡咯/金纳米电催化剂介导的信号放大技术检测 miRNA-21 的无标记超灵敏电化学生物传感器。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-10-07 DOI: 10.1039/d4ay01215a

LiNa Zhang, YanBin He

引用次数: 0

Analysis of synthetic route and case correlation of methamphetamine crystals seized by ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) and Pearson correlation coefficient method. 利用超高效液相色谱-质谱联用仪（UPLC-MS/MS）和皮尔逊相关系数法分析缉获的甲基苯丙胺晶体的合成途径和案件相关性。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-10-07 DOI: 10.1039/d4ay01519c

Shan Liu, Yuemeng Chen, Fang Wang, Song Bai, JingJie Wu

{"title":"Analysis of synthetic route and case correlation of methamphetamine crystals seized by ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) and Pearson correlation coefficient method.","authors":"Shan Liu, Yuemeng Chen, Fang Wang, Song Bai, JingJie Wu","doi":"10.1039/d4ay01519c","DOIUrl":"https://doi.org/10.1039/d4ay01519c","url":null,"abstract":"Analysis of impurities in illicit drugs provides key information on the manufacture and distribution of methamphetamine (MA). An analysis of 22 specific target compounds in unlawful MA products was conducted using ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS). The peak area of the alkaloids was utilized as an important calculation data variable to determine the synthesis method. The results demonstrated that the application of target screening techniques for trace impurity components in samples can aid in determining the synthesis process routes for toxin production in 169 samples. These samples were initially categorized based on their synthesis modes, specifically synthesis routes N, E, and R. The correlation analysis of MA samples with known synthetic routes was carried out using principal coordinate analysis (PCoA) and the Pearson correlation coefficient method. Among the N and R synthesis routes, 15 and 7 groups with strong correlation were screened, respectively. Consistent with the observed scenario, the samples from the synthetic route E showed limited correlation with each other. The experiment provides a focused framework for the management of synthetic raw materials used for the production of drugs and the investigation of drug-related cases. This facilitates a holistic strategy to combat drug-related crimes, from manufacturing laboratory to end-user.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142379513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification of creatinine in whole blood by a paper-based device using an RGB sensor. 使用 RGB 传感器的纸质设备定量检测全血中的肌酐。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-10-07 DOI: 10.1039/d4ay01476f

Neha Gautam, Ranjana Verma, Priya Ranjan Muduli, Shantimoy Kar, Arnab Sarkar

{"title":"Quantification of creatinine in whole blood by a paper-based device using an RGB sensor.","authors":"Neha Gautam, Ranjana Verma, Priya Ranjan Muduli, Shantimoy Kar, Arnab Sarkar","doi":"10.1039/d4ay01476f","DOIUrl":"https://doi.org/10.1039/d4ay01476f","url":null,"abstract":"Creatinine, an important biomarker for renal functions, is often conventionally estimated using the gold standard Jaffe reaction from blood, which involves the usage of a spectrophotometric measurement, thus restricting its utilization scope in point-of-care settings. Here, we report the development of a method for the single-step quantification of creatinine from whole blood using a paper-based microfluidic device. Our platform uses Whatman filter paper integrated with an LF1 membrane. The on-chip separation of blood plasma is achieved through the LF1 membrane, while the Whatman component of the device contains the embedded reagents for the Jaffe reaction. The combination of two different grades of paper enables a single-step quantification of creatinine as the separated blood plasma traverses to the reaction zone through capillary imbibition. Colorimetric readouts were quantified using an RGB sensor instead of a smartphone, which is highly platform dependent and incurs a relatively higher cost compared to the other components in typical point-of care (POC) devices. Our sensor was integrated within a 3D box, thereby making the detection virtually instrument free and perfectly suited for POC settings. The limit of detection (LOD) of our device was 0.219 mg dL-1, which falls within the lower range of physiological values. The coefficient of determination (R2) for the linearity and median accuracy were 0.978 and 94.047%, respectively. The relative standard deviation (RSD) for precision measurements remained below 5% for the developed protocol. Furthermore, we validated the performance of our device with 35 clinical samples in laboratory settings against the gold standard measurements. Our Bland-Altman plot as well as t-test and chi-square test results clearly confirmed the validity of our device within a 95% confidence interval.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142379515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fruit waste-derived carbon dots with rhodamine B for the ratiometric detection of Fe³⁺ and Cu². 水果废料衍生碳点与罗丹明 B 的比率法检测 Fe3+ 和 Cu2。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-10-04 DOI: 10.1039/d4ay01539h

Junyu Zhang, Yi Chen, Yiyang He, Yiwen Bai, Wei Wang, Guangxin Yang, Cong Kong, Xiao Cao, Lin Gu

{"title":"Fruit waste-derived carbon dots with rhodamine B for the ratiometric detection of Fe3+ and Cu2.","authors":"Junyu Zhang, Yi Chen, Yiyang He, Yiwen Bai, Wei Wang, Guangxin Yang, Cong Kong, Xiao Cao, Lin Gu","doi":"10.1039/d4ay01539h","DOIUrl":"https://doi.org/10.1039/d4ay01539h","url":null,"abstract":"A green and eco-friendly solvothermal approach is proposed for the synthesis of carbon quantum dots (CQDs) from watermelon rind. The as-prepared CQDs exhibited superior teal fluorescence in aqueous solutions, with a quantum yield of 13.9%. The CQDs and rhodamine B (RhB) were demonstrated to selectively react with Fe3+ and Cu2+, leading to a fluorescence (FL) quenching effect, which was successfully used for constructing \"double-response-off\" type ratiometric FL probes. A comparative study was conducted to assess the sensitivity and accuracy of ratiometric fluorescent probes, specifically those based on CQDs alone and in combination with RhB, for the selective detection of Fe3+ and Cu2+. By plotting the ratio of the differential fluorescence (ΔF) signals of CQDs to that of RhB against the practical application analyte concentration, the detection limits for Fe3+ (1.75 μM) and Cu2+ (0.43 μM) were markedly improved. The quenching mechanism was further explored, and the detection of Fe3+ and Cu2+ in surface water was demonstrated, showcasing the potential of efficient and effective nanosensors based on a static quenching effect. Futhermore, the addition of ascorbic acid can restore the fluorescence quenched by Fe3+. Therefore, in the presence of copper and iron, the ratiometric probe can demonstrate the ability to identify two different metals.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rational design of monodispersed Au@Pt core-shell nanostructures with excellent peroxidase-mimicking activity for colorimetric detection of Cr(VI). 合理设计具有优异过氧化物酶模拟活性的单分散 Au@Pt 核壳纳米结构，用于比色检测 Cr(VI)。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-10-04 DOI: 10.1039/d4ay01334d

Taohong Zhou, Jin Lin, Fengmei Jin, Yanxing Yao, Zhihao Li, Lihua Wang

引用次数: 0

A porphyrin-modified CoMoO₄ nanosensor array for the detection of crude baijiu. 用于检测粗白酒的卟啉修饰 CoMoO4 纳米传感器阵列。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-10-04 DOI: 10.1039/d4ay01082e

Peng Zhao, Xuhui Xia, Yiyao Luo, Zirui Yuan, Jiaxi Deng, Huibo Luo, Xiaogang Luo, Danqun Huo, Changjun Hou

引用次数: 0

Correction: Selective fluorescence detection of acetylsalicylic acid, succinic acid and ascorbic acid based on a responsive lanthanide metal fluorescent coordination polymer. 更正：基于响应性镧系金属荧光配位聚合物的乙酰水杨酸、琥珀酸和抗坏血酸的选择性荧光检测。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-10-04 DOI: 10.1039/d4ay90110j

Guo-Ying Chen, Mao-Ling Luo, Li Chen, Jia-Li Wang, Tong-Qing Chai, Dan Wang, Feng-Qing Yang

引用次数: 0

Combined detection of hepatitis B virus surface antigen and hepatitis B virus DNA using a DNA sensor. 使用 DNA 传感器联合检测乙型肝炎病毒表面抗原和乙型肝炎病毒 DNA。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-10-04 DOI: 10.1039/d4ay01629g

Hang Gong, Shufen Yao, Yong Li, Chunyan Chen, Feng Chen, Changqun Cai

{"title":"Combined detection of hepatitis B virus surface antigen and hepatitis B virus DNA using a DNA sensor.","authors":"Hang Gong, Shufen Yao, Yong Li, Chunyan Chen, Feng Chen, Changqun Cai","doi":"10.1039/d4ay01629g","DOIUrl":"https://doi.org/10.1039/d4ay01629g","url":null,"abstract":"Hepatitis B virus (HBV) infection is associated with the progression of liver disease. Occult HBV infection (OBI) is defined as the existence of detectable HBV DNA in HBV surface antigen negative individuals. However, HBV DNA is negative in serum while HBV surface antigen remains positive in incompletely cured chronic HBV infection. Hence, combined detection of HBV surface antigen and HBV DNA is essential for the accurate detection and rehabilitation of HBV infection. Therefore, a multiplex detection strategy based on branched DNA nanostructures was developed. The single-stranded segment of a branched DNA nanostructure (segments of S4 and S2) assembled by four single-stranded DNA was hybridized with the aptamer of HBV surface antigen and DNA hairpin to construct a DNA nanosensor, which can achieve high specificity identification and highly sensitive fluorescence responses to the targets. The detection limits of the developed nanosensor for HBV and HBV DNA are 50 pM and 5 nM, respectively. The combined detection of HBV surface antigen and HBV DNA provides a new insight for more thorough diagnostic evaluation of HBV infection and rehabilitation.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cost-effective quantification of uric acid using niobium oxide and graphene oxide-modified pencil-drawn electrodes on PVC substrates. 使用聚氯乙烯基底上的氧化铌和氧化石墨烯改性铅笔绘制电极进行尿酸定量分析，具有成本效益。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-10-03 DOI: 10.1039/d4ay01345j

Douglas P M Saraiva, Bruno Ferreira, Leonardo M A Ribeiro, Thiago R L C Paixão, Mauro Bertotti

{"title":"Cost-effective quantification of uric acid using niobium oxide and graphene oxide-modified pencil-drawn electrodes on PVC substrates.","authors":"Douglas P M Saraiva, Bruno Ferreira, Leonardo M A Ribeiro, Thiago R L C Paixão, Mauro Bertotti","doi":"10.1039/d4ay01345j","DOIUrl":"10.1039/d4ay01345j","url":null,"abstract":"This study introduces a cost-effective approach for quantifying uric acid (UA), the main antioxidant species in human physiology and implicated in inflammatory regulation. Using a PVC substrate and pencil drawing technique, electrodes were fabricated and modified with niobium oxide and graphene oxide via a straightforward \"drop casting\" method. The nanostructures of the substrate, electrode, and modified electrode were evaluated using SEM images. The synergistic effect between these materials significantly facilitated the uric acid oxidation process with a 400 mV peak potential shift and 45% current increase. The evaluation of the electrode's response to common blood and urine components showed minimal deviation. Among the components tested-ascorbic acid, glucose, nitrate, nitrite, cysteine, urea, creatinine, and ammonium ion-only the ammonium ion exhibited a 10% interference at concentrations commonly found in urine. The sensors showed a good detection limit of 8.7 μmol L-1, with a wide linear range from 8.7 to 2000 μmol L-1 with a correlation factor of 0.9993 for five different sensors. The reproducibility and repeatability of the produced sensors were estimated by the RSD at 4% and 1%, respectively. Synthetic urine samples spiked exhibited reliable analysis, with recovery values within a 5% error margin. This work presents a practical, simple, and affordable sensor platform for rapid and accurate UA quantification.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142363518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0