Analytical Methods最新文献

{"title":"Optimization and development of a green high performance liquid chromatography method for determination of 8 anti-epileptic drugs and 2 active metabolites in human serum assessed using AGREE, AGREEprep, GAPI and BAGI.","authors":"Xiao-Han Peng, Fan Zhang, Xin-Feng Xia, Kun Wang, Yan-Lin Zhao, Yan Du, Dao-Quan Tang","doi":"10.1039/d5ay00506j","DOIUrl":"https://doi.org/10.1039/d5ay00506j","url":null,"abstract":"<p><p>Pharmacological treatment with antiepileptic drugs (AEDs) remains the primary choice for most patients with epilepsy, while therapeutic drug monitoring is an important strategy to ensure the efficacy and safety of AEDs. The aim of this study was to optimize and develop a green high-performance liquid chromatography (HPLC) method suitable for simultaneous detection of 8 AEDs and 2 active metabolites including primidone (PRM), lamotrigine (LTG), carbamazepine (CBZ) and its active metabolite 10,11-epoxide (CBZE), oxcarbazepine (OXC) and its active metabolite 10,11-dihydro-10-hydroxyl carbamazepine (MHD), phenobarbital (PHB), eslicarbazepine acetate (ESLA), clonazepam (CZP) and diazepam (DZP) in human serum. The results showed that using chlorzoxazone as the internal standard, ethanol as the protein precipitant of serum, a ZORBAX Eclipse Plus-C18 (150 × 4.6 mm, 3.5 μm) column as the stationary phase, and gradient ethanol and 15 mM potassium dihydrogen phosphate water solution as the green alternative mobile phase, good chromatographic separation for 10 target analytes could be obtained. The calibration curves of 10 analytes demonstrated good linearity within the test range (<i>r</i> > 0.996). The intra- and inter-batch precision and accuracy were all less than 15%, while extraction recoveries were in the range of 74.57-90.89% with relative standard deviation values less than 15%. The results of greenness assessment showed that the proposed method was ecofriendly. Finally, the validated method was successfully applied in the analysis of real serum samples.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Cu₂O/hemin-GO nanozyme with aptamer-enhanced peroxidase-mimic activity for colorimetric detection of kanamycin in milk.","authors":"Yaoting Mou, Lu Han, Yanhao Yin, Jianghua Liu, Yanqing Xu, Yuhang Tian, Yanfang Wu, Dongfei Chen, Yemin Guo, Xia Sun, Falan Li","doi":"10.1039/d5ay00594a","DOIUrl":"https://doi.org/10.1039/d5ay00594a","url":null,"abstract":"<p><p>Kanamycin (KAN) is an aminoglycoside antibiotic employed for the treatment of bacterial infections in livestock and poultry and is widely used as a veterinary drug in animal husbandry. Its overuse poses serious threats to public health and ecosystems; while traditional detection methods cannot meet the requirements of widespread application and rapid detection, developing rapid and reliable technology for KAN detection is essential. In this study, a colorimetric aptasensor was developed to rapidly detect KAN residues, leveraging the enhanced catalytic effect of aptamers on the peroxidase-like activity of the Cu₂O/hemin-graphene oxide (Cu₂O/hemin-GO) nanozyme. In the presence of hydrogen peroxide, the Cu₂O/hemin-GO nanozyme demonstrated excellent peroxidase-like catalytic activity, oxidizing 3,3',5,5'-tetramethylbenzidine (TMB). The adsorption of aptamers on the nanozyme surface increased the negative charge density and significantly enhanced the affinity of the nanozyme for the positively charged chromogenic substrate TMB, boosting the catalytic activity nearly threefold. Upon exposure to KAN, the specific binding of aptamers to KAN reduced their adsorption on the Cu₂O/hemin-GO nanozyme, leading to a decrease in the peroxidase-like activity of the Cu₂O/hemin-GO nanozyme and a corresponding reduction in color change. The aptasensor exhibited excellent sensitivity with a linear detection range from 0.1 pM to 1 μM and a detection limit of 16 fM. The recovery rates for KAN in milk samples ranged from 92.05% to 110.71%. Furthermore, the colorimetric aptasensor demonstrated high selectivity and reproducibility. Overall, the colorimetric approach achieved the economical, simple and sensitive detection of KAN residues, indicating its promising potential in real-world applications. In addition, this sensing method could be applied to other targets by replacing the aptamer, providing a strategy for developing sensors facilitating rapid detection.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A stable Zn-coordination polymer for the quantitative and selective detection of biomarker phenylglyoxylic acid in urine.","authors":"Rongqi Wang, Ruxue Wang, Zeqin Chen, Laiwen Li, Xinyu Zong, Jing Wang, Ziao Zong","doi":"10.1039/d5ay00405e","DOIUrl":"https://doi.org/10.1039/d5ay00405e","url":null,"abstract":"<p><p>Developing stable sensing materials for the selective detection of phenylglyoxylic acid (PGA) in biological samples is highly important for the early diagnosis and treatment of various diseases. In this work, a Zn(II)-based coordination polymer (CP), {[Zn(L)(bibp)]·bibp·2H₂O}_<i>n</i> (1) (H₂L = 2,6-di(4-carboxylphenyl)-4-(4-(triazol-1-ylphenyl))pyridine and bibp = 4,4'-bis(imidazolyl)biphenyl), with good chemical and thermal stabilities was solvothermally synthesized. It showed an excellent fluorescence turn-off response capability for the detection of the styrene biomarker PGA with outstanding sensitivity, selectivity, recoverability and anti-interference ability. Moreover, experimental and DFT calculations suggested that the mechanism of fluorescence quenching could be attributed to the synergistic effect of the internal filter effect (IFE) and photo-induced electron transfer (PET) process between the complex and PGA.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combination of on-chip electromembrane extraction and solid phase microextraction for determination of non-steroidal anti-inflammatory drugs from biological fluids using poly(methacrylic acid-ethylene glycol dimethacrylate)-Cu/Cr layered double hydroxide composite as a sorbent.","authors":"Razieh Zamani, Yadollah Yamini, Monireh Karami","doi":"10.1039/d5ay00557d","DOIUrl":"https://doi.org/10.1039/d5ay00557d","url":null,"abstract":"<p><p>In this study, an innovative combination of on-chip electromembrane extraction and solid-phase microextraction (EME-SPME) was proposed for the first time. To develop this system, a monolithic composite of poly(methacrylic acid-ethylene glycol dimethacrylate)-Cu/Cr layered double hydroxide was <i>in situ</i> synthesized in the acceptor channel. This microfluidic device was employed in the determination of non-steroidal anti-inflammatory drugs, including naproxen, diclofenac, and mefenamic acid, in human biological fluids. During the extraction procedure, the immigration of compounds driven by an electric field and subsequent adsorption into the solid phase were achieved simultaneously. Likewise, the desorption stage was performed subsequently, and an eluent was introduced into HPLC. Optimization of key parameters affecting the extraction efficiency of the analytes was performed to achieve the highest extraction efficiency. Under optimized conditions, the method demonstrated detection limits between 0.1 and 0.25 μg L^-1 for the target analytes. The obtained linearity was within the range of 0.5-500 μg L^-1 for naproxen and 1-250 μg L^-1 for diclofenac and mefenamic acid (<i>R</i>² ≥ 0.9962). The method provided acceptable precision (RSDs ≤ 8.1%) and notable extraction recoveries of 84.3-90.9%, corresponding to preconcentration factors of 56-61. The proposed method was applied to extract the target analytes from breast milk, urine, and plasma samples, yielding satisfactory results. Eventually, the employment of a sorbent in the acceptor phase with its macroporous monolithic structure and the integration of two methods were demonstrated to be beneficial to increase efficiencies based on the achieved results.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a new biodetection system independent of known marker molecules using a novel material for microarrays made from amorphous carbon substrates.","authors":"Yuki Tominaga, Kiyoshi Nokihara","doi":"10.1039/d5ay00426h","DOIUrl":"https://doi.org/10.1039/d5ay00426h","url":null,"abstract":"<p><p>A bio-chip substrate made from amorphous carbon was successfully developed as an innovative and practical biodetection system. This peptide-microarray biochip was based on a novel principle for protein detection in diagnostics. The carbon material as a substrate carrier offered significant advantages over conventional glass slides with regard to its surface chemistry. The biochip detection system used the measurement of fluorescent intensity changes caused by the interactions between capture molecules (labelled structured peptides arrayed on the substrate) and analytes. Fluorescent intensity changes with and without analytes were dose-dependent and could be visualized as \"protein fingerprints\". In this recognition system, interactions are not limited to one-to-one correspondence as in ELISA. Hence, this method can even be applied to analytes containing uncharacterized molecules. Statistical data handling, such as multivariate analyses, enabled discrimination of complex analytes such as body fluids. The present biochip used with an in-house developed detection device could be re-used 10-20 times with the same or different analytes. The captured molecules in this system were immobilized on the chip-surface through stable amide bonds, which allowed washing and even scrub cleaning. This unique material has a potential for dual detection thorough fluorescent intensity changes and mass spectrometric analyses when the chip is used as a sample tray, since this material has high electric conductivity. Thus, this dual detection allows the possibility of discovering marker candidates.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-dimensional profiling and peptide sequencing of angiotensin-I converting enzyme (ACE) inhibitory proteolysate from winged bean (<i>Psophopcarpus tetragonolobus</i>) seeds.","authors":"Shyan Yea Chay, Fatema Hossain Brishti, Shehu Muhammad Auwal, Zulhisyam Abdul Kari, Nurdiyana Aqilah Roslan, Clement Kiing Fook Wong, Nazamid Saari","doi":"10.1039/d4ay02073a","DOIUrl":"https://doi.org/10.1039/d4ay02073a","url":null,"abstract":"<p><p>Winged bean seed (WBS) is an underutilised tropical leguminous crop that represents a green, sustainable source of plant protein. The potential of WBS to produce biofunctional peptides, particularly ACE-inhibitory peptide, remains unexplored. In the current work, the seeds were enzymatically proteolysed using papain, and the proteolysate was separated <i>via</i> a two-step mechanism (reverse-phase high performance liquid chromatography and isoelectric focusing). A peptide search was conducted <i>via</i> sequencing using liquid chromatography with tandem mass spectrometry. Three subfractions from isoelectric focusing, namely F-12-12 (ACE inhibition rate = 87.8%; peptide content = 1.0 mM; isoelectric point, pI = 10.0), F-16-2 (ACE inhibition rate = 50.1%; peptide content = 0.4 mM; pI = 3.6) and F-16-6 (ACE inhibition rate = 77.3%; peptide content = 0.9 mM, pI = 6.8) were selected for sequencing. Based on the structural requirements for a peptide to exhibit ACE-inhibitory properties, as well as the confidence score obtained during sequencing, a total of eleven peptides (TSISSEDKTPPQPRN, ISSEDKTPPQPR, SEDKTPPQPR, SISSEDKTPPQPR, ADQLDTAR, DVKERAKDYG, TASSVEEAT, ERAKDYG, STNPIT, RGVFPCLK, TQLDLPTQ) and four peptides (LSSEDKTP, EPALVP, MRSVVT, DMKP) were successfully identified upon a database search and <i>de novo</i> sequencing, respectively. All peptides were oligopeptides, with low molecular weights of <2 kDa (specifically, 489.9-1656.7 Da) and contained 4-15 amino acid residues. An in-depth technical interpretation of the sequencing parameters was subsequently elucidated. In short, the current work demonstrates the potential of the sustainable plant protein source WBS to produce ACE-inhibitory peptides, which could ultimately aid in blood pressure regulation, and concurrently sheds light on the technical fundamentals of peptide separation and sequencing.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Critical analysis of advanced nanosensors for dengue detection.","authors":"Renu Poria, Ankur Kaushal, Narinder Kaur, Deepak Kala, Gajanan Ghodake, Shagun Gupta, Deepak Kumar","doi":"10.1039/d4ay02241f","DOIUrl":"https://doi.org/10.1039/d4ay02241f","url":null,"abstract":"<p><p>Dengue, a major global health risk, is a virus spread by <i>Aedes</i> aegypti and <i>Aedes albopictus</i> mosquitoes. It is most common in subtropical and tropical areas. For efficient epidemic management and control, dengue virus (DENV) identification must be performed accurately and in a timely manner. Traditional diagnostic techniques, such as molecular detection, antigen detection, and serological testing, have several drawbacks, including being expensive and time-consuming, having high turnaround times, having low sensitivity, and showing delayed results. With the advent of nanotechnology, these issues can be addressed by developing nanosensors with enhanced sensitivity, specificity, and fast detection rates. A comprehensive investigation of nanosensor applications in DENV detection is provided in this article. The dengue virus is briefly discussed, along with its composition and life cycle and drawbacks of the current diagnostic methods. After that, we evaluate the fundamental ideas behind nanosensors and how they work with contemporary technologies to identify diseases. The main approaches, including using various types of nanosensors, such as optical and fluorometric nanobiosensors, colorimetric nanosensors, and surface-enhanced Raman scattering (SERS) biosensors, impedimetric nanobiosensor-based detection methods, electrochemical and piezoelectric-based detection methods, microfluidic-based and nonconventional microfluidic-based detection of the dengue virus, CRISPR-based assays, DNA switch-based sensing technologies and using smartphone-based biosensors, are discussed in detail, highlighting their mechanisms and applications in the context of dengue detection. There is an emphasis on the benefits of these nanosensors in terms of quick, precise and affordable diagnosis. The discussion then shifts to the current challenges faced in the application of nanosensors for dengue detection. Finally, we outline future directions and emerging trends in the field, outlining possible avenues for research and development to overcome existing barriers and improve the application of nanosensors in clinical settings.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural characterization and quantification of isomeric bioactive flavonoid C-glycosides utilizing high-performance liquid chromatography coupled with diode array detection and mass spectrometry.","authors":"Arvind Kumar Maurya, Ashish Singh Tanwar, Dipak K Mishra, Sanjeev Kanojiya","doi":"10.1039/d5ay00398a","DOIUrl":"https://doi.org/10.1039/d5ay00398a","url":null,"abstract":"<p><p>Flavonoid C-glycosides are secondary metabolites that occur selectively in plants. They are recognized for their antioxidant and broad range of biological properties. A recent study has reported that isoorientin regulates the GSK3β, NF-κB and Nrf2/HO-1 signalling pathways to exert neuroprotection. In this study, isomeric bioactive flavonoid C-glycosides were structurally characterized as isoorientin, orientin, vitexin, and isovitexin using tandem mass spectrometry. Furthermore, a simple isocratic, and robust RP-HPLC analytical method was developed and validated for the simultaneous quantification of these isomeric flavonoid C-glycosides in multiple plant matrices. The chromatographic separation was achieved on a C18 (150 × 4.6 mm, 3.5 μm) column at a flow rate of 1.2 mL min^-1. Acetonitrile and buffer (0.1% formic acid in a 95% water-methanol mixture) with 12 : 88 v/v were used as the mobile phase. The retention times (<i>t</i>_R) of isoorientin (9.23 ± 0.07 min), orientin (10.46 ± 0.09 min), vitexin (16.00 ± 0.13 min) and isovitexin (17.35 ± 0.10 min) were observed and quantified successfully. The limit of detection (LOD) and limit of quantification (LOQ) for the analytes were 2.53-4.31 and 7.66-13.08 μg mL^-1, respectively, while regression coefficients (<i>r</i>²) were found to be between 0.99989 and 0.99996. The abundance of isoorientin, orientin, vitexin and isovitexin was determined to be 13.25-94.85, 4.00-106.25, 2.16-25.03 and 7.56-14.14 mg g^-1 (w/w), respectively, in multiple matrices of <i>Cajanus scarabaeoides</i> and <i>Rhynchosia minima</i>. The developed isocratic HPLC method is the first to be reported and validated for its precision, accuracy, repeatability, and stability for the simultaneous quantification of flavonoid C-glycosides in six plant matrices.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A gold nanoparticle-based colorimetric strategy for DNA detection: principles and novel approaches.","authors":"Nguyen Tran Truc Phuong, Hanh An Nguyen, Thi Ngoc Diep Trinh, Kieu The Loan Trinh","doi":"10.1039/d5ay00148j","DOIUrl":"https://doi.org/10.1039/d5ay00148j","url":null,"abstract":"<p><p>The development of nanotechnology has led to the rapid growth of many different fields, including sensors. Bulky and complex sensor systems are gradually being replaced by streamlined sensor devices with advantages in size, simplicity, cost-effectiveness, and fast response, allowing qualitative detection of target analyte on-site application for clinical diagnosis. Significantly, since the COVID-19 pandemic, research on developing test kits for detecting biological molecules has grown rapidly, with an increasing number of publications. The number of studies developing colorimetric sensors based on gold nanoparticles (AuNPs) has increased continuously over the years, demonstrating the potential application of this material. The surface plasmon resonance (SPR) effect and high biocompatibility of AuNPs make them different from many other metal nanomaterials. In addition, the peroxidase activity properties of AuNPs have also received much attention in colorimetric sensors. In this review, the colorimetric sensors developed based on the AuNP material platform for DNA detection will be discussed in detail. Among them, the commonly used synthesis methods of AuNPs based on their applications and the primary mechanism of AuNP-based colorimetric sensors for DNA detection will be discussed. In addition, AuNP-based colorimetric applications in POCT for pathogenic bacteria and viruses are also mentioned in this review to provide a broader perspective on the potential and developmental direction of AuNP-based colorimetric sensors. Another aspect this review provides is development strategies that allow simple readout using the naked eye, a spectrophotometer, or a smartphone camera, which present many opportunities for integration into other electronic devices.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on primary microplastics in cosmetics: their isolation, spectral and thermal analysis.","authors":"Ivan M Zorin, Pavel S Chelushkin, Anna S Senchukova, Petr A Fetin, Jose M Kenny, Sergey V Lyulin","doi":"10.1039/d5ay00178a","DOIUrl":"https://doi.org/10.1039/d5ay00178a","url":null,"abstract":"<p><p>Eight samples of personal care and cosmetic products (PCCPs) available on the market were analyzed to isolate and characterize intentionally added polymer particles. In four of the samples, particles of synthetic polymers (microplastics, MP) were detected. Among the various types of PCCPs, the most abundant MP sources were glitter gels and hand cleaning pastes. Results demonstrate that the combination of FTIR and Raman spectroscopy, which is the most widely used approach for MP identification, is not always sufficient for the accurate detection and differentiation of synthetic polymers in the products analyzed. The use of independent methods (solubility tests and DSC thermal analysis) allowed us to achieve additional important information and helped prevent incorrect conclusions regarding the presence of microplastics in PCCPs. DSC thermal analysis proved to be particularly useful in cases wherein a combination of spectroscopic methods was ineffective in MP identification.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}