Analytical Methods最新文献_第2页

High-throughput plasma/serum proteomics by coupling nanoparticle protein corona-based sample preparation and capillary zone electrophoresis-tandem mass spectrometry. 高通量血浆/血清蛋白质组学通过耦合纳米颗粒蛋白冠状样品制备和毛细管带电泳-串联质谱。

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-07-29 DOI: 10.1039/d5ay00721f

Qianyi Wang, Seyed Amirhossein Sadeghi, Fei Fang, Dejin Zheng, Chenxiang Luo, Guangyao Gao, Qianjie Wang, Bin Gu, Liangliang Sun

{"title":"High-throughput plasma/serum proteomics by coupling nanoparticle protein corona-based sample preparation and capillary zone electrophoresis-tandem mass spectrometry.","authors":"Qianyi Wang, Seyed Amirhossein Sadeghi, Fei Fang, Dejin Zheng, Chenxiang Luo, Guangyao Gao, Qianjie Wang, Bin Gu, Liangliang Sun","doi":"10.1039/d5ay00721f","DOIUrl":"10.1039/d5ay00721f","url":null,"abstract":"A high-throughput workflow for bottom-up proteomics (BUP) of human plasma using capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) and nanoparticle protein corona-assisted sample preparation is presented. The streamlined approach enabled the identification and quantification of hundreds of proteins from plasma/serum samples in 3.5 hours from sample to data. Nanoparticles with varied physiochemical properties studied in this work captured different pools of the plasma/serum proteome in the protein coronas, and the protein corona-based sample preparation approach enabled the measurement of low-abundance proteins compared to the approach without nanoparticles. Applying this high-throughput workflow to serum samples of a mouse NUT carcinoma (NC) cancer model allowed the determination of differentially expressed serum proteins between NC bearing mice and healthy controls. By comparing our quantitative proteomics data with published transcriptomics data, we revealed a handful of potential serum protein biomarkers of NC cancer (e.g., secreted phosphoprotein 1, SPP1). We expect this high-throughput workflow, with additional improvement in the speed of the mass spectrometer, will be useful for advancing the discovery of new protein biomarkers of diseases (e.g., cancer) using plasma/serum samples.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12305297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound-assisted ionic liquid microextraction and colorimetric analysis for trace-level gold detection in environmental and geological samples. 超声辅助离子液体微萃取和比色分析在环境和地质样品中的痕量金检测。

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-07-29 DOI: 10.1039/d5ay01045d

Fahad M Alminderej, Alaa M Younis, Alaa S Amin, Hesham H El-Feky

{"title":"Ultrasound-assisted ionic liquid microextraction and colorimetric analysis for trace-level gold detection in environmental and geological samples.","authors":"Fahad M Alminderej, Alaa M Younis, Alaa S Amin, Hesham H El-Feky","doi":"10.1039/d5ay01045d","DOIUrl":"https://doi.org/10.1039/d5ay01045d","url":null,"abstract":"A novel, precise, and targeted method has been developed for isolating, preconcentrating, and analyzing gold ions in water, ore, and food samples. This approach utilizes ultrasound-assisted dispersive liquid-liquid microextraction based on ionic liquids, combined with colorimetric detection of a distinct deep red hue. The compound 2-amino-4-(m-tolylazo)pyridine-3-ol (ATAP), introduced for the first time as a gold-specific chelating agent, was employed alongside the ionic liquid 1-octyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide in an ultrasonic bath maintained at 25 ± 2 °C for extraction. Following centrifugation, the gold complex was enriched within the ionic liquid droplets, and 200 μL of ethanol was added to the ionic liquid-rich layer prior to colorimetric analysis. Key variables influencing the recovery of Au3+ ions were systematically examined and fine-tuned to achieve optimal results. After fine-tuning both the instrumental and experimental conditions, the proposed method demonstrated a linear dynamic range for Au3+ ions between 4.0 and 240 ng mL-1, with high molar absorptivity and a Sandell sensitivity of 1.22 × 106 L mol-1 cm-1 and 0.016 ng cm-2, respectively. The method achieved quantification and detection limits of 3.92 and 1.30 ng mL-1, respectively. A preconcentration factor of 500 was obtained. The relative standard deviation (RSD) was calculated to be 1.89% for gold ions at a concentration of 100 ng mL-1 (n = 10). No significant interference from various anions and cations was detected during the assessment of Au3+. These findings confirm that the proposed technique is a fast, straight-forward, highly sensitive, selective, and effective analytical approach for isolating and quantifying Au3+ ions in environmental and geological samples, all while using minimal solvent volumes.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-response fluorescent carbon dots for Ag⁺ and Hg²⁺ detection study in actual samples and biological systems. 双响应荧光碳点在实际样品和生物系统中检测Ag+和Hg2+的研究。

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-07-29 DOI: 10.1039/d5ay00688k

Xinyan Liu, Chao Liu, Qingyue Hu, Huihui Ge, Jie Tan, Jianhua Wang, Wei Bian

{"title":"Dual-response fluorescent carbon dots for Ag+ and Hg2+ detection study in actual samples and biological systems.","authors":"Xinyan Liu, Chao Liu, Qingyue Hu, Huihui Ge, Jie Tan, Jianhua Wang, Wei Bian","doi":"10.1039/d5ay00688k","DOIUrl":"https://doi.org/10.1039/d5ay00688k","url":null,"abstract":"It is important to be capable of achieving highly selective detection of Ag+ and Hg2+ because their excessive concentrations may pose a serious threat to human health and lead to a variety of diseases. Herein, a new type of orange fluorescent carbon dot with an emission wavelength of 590 nm was synthesized by a hydrothermal method using 1,2,4-triaminobenzene dihydrochloride and ethylenediamine as raw materials. The synthesized orange carbon dots have high affinity for Hg2+ and Ag+ ions, which can be selectively detected by fluorescence quenching with the detection limits of 0.37 nM and 0.65 nM, respectively. Moreover, the carbon dots have good fluorescence stability for the rapid detection of Ag+ and Hg2+ over a wide pH range. Importantly, the addition of EDTA reversibly restored the fluorescence intensity of the mixed solution of carbon dots and Hg2+, but not the fluorescence intensity of the Ag+-containing probe solution, which allowed for a clear distinction between Hg2+ and Ag+. In addition, the carbon dots have good biocompatibility and low toxicity to MCF-7 cells, which can be used for cell imaging and intracellular ion detection.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An improved system and analytical method for determining oxygen isotopes in ¹⁸O-enriched water samples. 富氧水样中氧同位素测定的改进系统和分析方法。

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-07-29 DOI: 10.1039/d5ay00505a

Hongwei Li, Lianjun Feng

{"title":"An improved system and analytical method for determining oxygen isotopes in 18O-enriched water samples.","authors":"Hongwei Li, Lianjun Feng","doi":"10.1039/d5ay00505a","DOIUrl":"https://doi.org/10.1039/d5ay00505a","url":null,"abstract":"The spatiotemporal differences in the hydrogen and oxygen isotopic composition of water offer a powerful technical approach for research on the water cycle, paleoclimate reconstruction, and water source identification. Hydrogen and oxygen isotopes in certain specialized samples, such as 18O-enriched water, have been utilized in food science, environmental ecology, and medical research. To address the low efficiency of traditional offline H2O-CO2 equilibrium methods and the pronounced memory effect associated with continuous-flow techniques for 18O-enriched water samples, we propose a novel analytical system and methodology for oxygen isotope determination in water. The system was independently designed and established in-house. A water equilibrium unit and an extraction preparation unit were organically combined in this system. A water bath shaker was designed for the equilibrium system to accelerate the complete equilibrium exchange and reduce reaction time. A 3.0 mL water sample was injected into a glass bottle and equilibrated with carbon dioxide gas in a water bath at 25 °C for 4 h. While maintaining a high analytical accuracy (within 0.10% and 0.50% for reference materials USGS45 and IAEA-608, respectively), this method significantly improves the efficiency, reducing the processing time by over 10 h. It offers a preferred solution for oxygen isotope analysis in water samples, particularly those enriched in 18O.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comonomer distribution analysis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)s using high-resolution MALDI-TOF mass spectrometry. 高分辨率MALDI-TOF质谱法分析聚（3-羟基丁酸酯-co-3-羟基己酸酯）的单体分布。

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-07-28 DOI: 10.1039/d5ay00910c

Naoki Kanayama, Sayaka Nakamura, Shin Sano, Mami Tanaka, Noriko Takahara, Hiroaki Sato, Hideaki Hagihara

{"title":"Comonomer distribution analysis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)s using high-resolution MALDI-TOF mass spectrometry.","authors":"Naoki Kanayama, Sayaka Nakamura, Shin Sano, Mami Tanaka, Noriko Takahara, Hiroaki Sato, Hideaki Hagihara","doi":"10.1039/d5ay00910c","DOIUrl":"https://doi.org/10.1039/d5ay00910c","url":null,"abstract":"In practical applications of poly(3-hydroxybutyrate) (PHB) copolyesters, the distribution of comonomer units within the polymer chains (i.e., comonomer distribution) is an important factor influencing various material properties, alongside the comonomer structure and content. However, analytical methodologies for the characterisation and representation of comonomer distribution in PHB copolyesters remain limited. In the present study, we examined the comonomer distribution in poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBH) using matrix-assisted laser desorption/ionisation spiral time-of-flight mass spectrometry (MALDI-spiral-TOF-MS). High-molecular-weight PHBH samples were partially methanolysed and subsequently fractionated by size-exclusion chromatography to yield oligomers of suitable molecular sizes for analysis. The high-resolution capabilities of MALDI-spiral-TOF-MS enabled the separation of ion peaks with similar m/z values in the PHBH oligomers and the determination of their accurate masses. The resulting mass spectral data were subsequently subjected to Kendrick mass defect (KMD) analysis to classify ion peaks according to their end-group structures, thereby facilitating the identification of distinct molecular species. Accurate mass measurements enabled the unambiguous determination of the corresponding molecular formulae, end groups, and monomer compositions of the PHBH oligomers. By organising the ion peak data corresponding to PHBH chains of a specific length (e.g., 21-mer), the distribution of 3-hydroxyhexanoate (3HH) units within the molecular chain was represented as a number distribution plot for that chain length. This analytical approach was also succesfully applied to PHBH film residues subjected to biodegradation in seawater.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A microfluidic paper-based analytical device based on a surface-modified screen-printed electrode Pt-Pd/RGO nanocomposite for glucose detection in urine. 基于表面修饰的丝网印刷电极Pt-Pd/RGO纳米复合材料的微流控纸基分析装置用于尿液中的葡萄糖检测。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-07-25 DOI: 10.1039/d5ay00852b

Chaozhan Chen, Ruhuan Ye, Zidong Chen, Jiayi Ye, Bin Ran, Bo Liu, Jialin Liang, Jiale Huang, Teng Shen

{"title":"A microfluidic paper-based analytical device based on a surface-modified screen-printed electrode Pt-Pd/RGO nanocomposite for glucose detection in urine.","authors":"Chaozhan Chen, Ruhuan Ye, Zidong Chen, Jiayi Ye, Bin Ran, Bo Liu, Jialin Liang, Jiale Huang, Teng Shen","doi":"10.1039/d5ay00852b","DOIUrl":"https://doi.org/10.1039/d5ay00852b","url":null,"abstract":"Urine biomarker detection with high accuracy and sensitivity is crucial for advancing point-of-care diagnostics. This paper developed a microfluidic paper-based analytical device (μPAD) for electrochemical glucose detection in urine. The proposed μPAD employs a filter paper substrate to enable pump-free sample transport to a detection zone integrated with the screen-printed electrode (SPE), allowing rapid quantification within 60 seconds. A composite of reduced graphene oxide (RGO)/platinum-palladium nanoparticles (Pt-Pd NPs) with good analytical performance has been electrodeposited on the surface of SPEs and subsequently combined with glucose oxidase (GOx) to create a glucose biosensor (GOx/Pt-Pd NPs/RGO/SPE). Under optimized conditions, the Pt-Pd NPs/RGO/SPE shows good performance, showing a 17.4-fold sensitivity enhancement over bare SPEs in H2O2 detection. The developed μPAD achieves wide linear ranges of 5 μM-20 mM (H2O2) and 20 μM-5 mM (glucose), with low detection limits of 0.97 μM and 8.1 μM, respectively. Furthermore, the μPAD shows wide linear ranges covering required detection ranges of glucose in urine, demonstrating its great potential in personal health monitoring and clinical applications requiring reliable glucose measurement in urine.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of an FR4 printed circuit board for the fabrication of a simple and low cost patterned Cu disk for the determination of paraquat. 应用FR4印刷电路板制作用于测定百草枯的简单低成本图案化铜盘。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-07-25 DOI: 10.1039/d5ay00824g

Narjes Negahdari, Behzad Haghighi

引用次数: 0

Development of a size exclusion chromatography cartridge-based analytical method for determination of free drug in nano-liposomal oncology drug formulations. 纳米肿瘤药物脂质体制剂中游离药物含量测定方法的建立。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-07-25 DOI: 10.1039/d4ay02124j

Wei Zhang, Mark Paciolla, Lijun Duan, Elise Bradley, Aastha Chadha, Bhavesh Barot, Kaylee Worrell

{"title":"Development of a size exclusion chromatography cartridge-based analytical method for determination of free drug in nano-liposomal oncology drug formulations.","authors":"Wei Zhang, Mark Paciolla, Lijun Duan, Elise Bradley, Aastha Chadha, Bhavesh Barot, Kaylee Worrell","doi":"10.1039/d4ay02124j","DOIUrl":"https://doi.org/10.1039/d4ay02124j","url":null,"abstract":"A reliable and stability-indicating size exclusion chromatography (SEC) cartridge-based free drug testing method was developed for active loading nanoliposome formulations through a systematic development approach. The SEC spin cartridge columns (7K MWCO, 2 mL) were used for developing the liposome free drug testing procedure. The SEC cartridge column retention capacity for a Mirati drug was determined (445 μg per cartridge). SEC testing conditions were studied to achieve a good separation between liposomes and the free drug, including cartridge conditioning, buffer wash steps for liposomal drug elution, organic media wash steps for free drug elution, and sample size effect. Qualification of this newly developed SEC cartridge method has demonstrated its specificity/selectivity without interference and excellent detection linearity (correlation coefficient (R) > 0.999) over a study concentration range (1.0 to 46.9 μg mL-1), sufficient LOQ (0.52 μg mL-1 equivalent to 1.7% of free drug in a liposome formulation at 2.5 mg mL-1), acceptable accuracy/recovery of 81 to 89% for free drug in spiked samples at 4.5%, 9%, and 18% levels (sample loading by a regular pipet) and 88 to 97% at 10% spike level (sample loading by a positive displacement pipet), good method precision (RSD (n = 6) of 4% NMT) for free drug determination, and 3 days solution stability for both standard and sample solutions (2-8 °C). In comparative sample testing, the SEC free drug test results were in good agreement with the solid phase extraction (SPE) test results for active loading formulations. The new method's stability-indicating ability has been proved through monitoring free drug in a liposome sample stored at -20 °C and -80 °C.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Golgi-targeting fluorescent probe for the detection of hydrazine in biological and environmental systems. 用于生物和环境系统中肼检测的高尔基靶向荧光探针。

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-07-25 DOI: 10.1039/d5ay00690b

Hui-Juan Lai, Xu Wang, Yuan Wang, Wei-Na Wu, Zhi-Hong Xu

引用次数: 0

Nondestructive presymptomatic detection of blue-fluorescing substances in tomato leaves infected with Ralstonia solanacearum using a polyvinyl alcohol hydrogel. 聚乙烯醇水凝胶法对茄枯病番茄叶片中蓝色荧光物质的症状前无损检测

IF 2.7 3区化学

Analytical Methods Pub Date : 2025-07-25 DOI: 10.1039/d5ay00579e

Shigeyuki Iwasa, Yuso Kobara, Katsumi Maeda, Arina Asano, Tatsuya Shimanuki, Kuniaki Nagamine

引用次数: 0