Analytical Methods最新文献

{"title":"BiFusionPathoNet: fusion network for drug-resistant bacteria identification <i>via</i> optical scattering patterns.","authors":"Yichuan Wang, Xu He, Mubashir Hussain, Luyao Ma, Jingjing Wang, Mingyue Chen, Na Yang, Xiuping Zhou, Chao Wang, Haiquan Kang, Bin Liu","doi":"10.1039/d4ay02074j","DOIUrl":"10.1039/d4ay02074j","url":null,"abstract":"<p><p>The presented research introduces a new method to identify drug-resistant bacteria rapidly with high accuracy using artificial intelligence combined with Multi-angle Dynamic Light Scattering (MDLS) signals and Raman scattering signals. The main research focus is to distinguish methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and methicillin-sensitive <i>Staphylococcus aureus</i> (MSSA). First, a microfluidic platform was developed embedded with optical fibers to acquire the MDLS signals of bacteria and Raman scattering signals obtained by using a Raman spectrometer. After that, for the detection of both scattering signals of MRSA and MSSA, three models were developed: (1) ResistNet, a hybrid model combining a Transformer Encoder with ResNet, with an accuracy of 83.8% on the MDLS dataset.; (2) SERB-CNN, which attained 91.84% accuracy on a Raman scattering public dataset and 93.5% on a custom-built dataset; and (3) BiFusionPathoNet, a multimodal fusion model that reached 96.8% accuracy, significantly outperforming single-modal approaches. The acquired results demonstrated the effectiveness of this multimodal strategy for the rapid detection of drug-resistant bacteria.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"1101-1110"},"PeriodicalIF":2.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a new method using dispersive liquid-liquid microextraction with hydrophobic natural deep eutectic solvent for the analysis of multiclass emerging contaminants in surface water by liquid chromatography-mass spectrometry.","authors":"Fernanda Volpatto, Luciano Vitali","doi":"10.1039/d4ay02012j","DOIUrl":"10.1039/d4ay02012j","url":null,"abstract":"<p><p>A new analytical method was developed for the determination of 14 multiclass emerging organic contaminants in surface waters using LC-MS, and Dispersive Liquid-Liquid Microextraction (DLLME) for extraction. Different Natural Deep Eutectic Solvents (NADESs) composed of terpenes and organic acids were tested as extraction solvents and characterized by Fourier Transform Infrared Spectroscopy (FTIR), Hydrogen Nuclear Magnetic Resonance Spectroscopy (¹H-NMR), Differential Scanning Calorimetry (DSC), Thermogravimetric Analysis (TGA), density, and viscosity, eliminating the need to use traditional chlorinated solvents. NADES produced with butyric acid and thymol showed the best results and was selected for application for the first time in the extraction of emerging organic contaminants of different classes in water samples. Vortex was used as the dispersion mode, eliminating the use of the dispersion solvent. Chromatographic conditions and sample preparation were optimized using multivariate experimental designs. The optimized chromatographic conditions included the column oven temperature, mobile phase modifiers, and stationary phase type. The optimized conditions for sample preparation included the extraction temperature and pH, salting out effect, and extraction solvent volume. The analytical performance was evaluated through repeatability and intermediate precision tests, with RSD values below 20%, and recoveries between 70 and 120%. The coefficient of determination was greater than 0.98 for all analytes. LOQs varied between 1.5 and 35 μg L^-1. DLLME is a simple technique, it does not require expensive and specific equipment. Furthermore, replacing traditional chlorinated solvents with NADES makes the procedure more environmentally friendly. The method presented here can be applied to a wide range of analytes for the analysis of fresh, brackish, and salt waters. Up to the present moment, this is the first study using NADES based thymol and butyric acid for the determination of multiclass emerging contaminants in surface waters samples.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"1032-1046"},"PeriodicalIF":2.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Galactose oxidase oxidation and glycosidase digestion for glycoRNA analysis.","authors":"Jianbo Deng, Xinyu Miao, Xiaotong Wang, Sheng-Ye Wen, Zeyang Zhou, Shuwei Li, Junhong Jiang, Xiaodong Yang, Shuang Yang","doi":"10.1039/d4ay02046d","DOIUrl":"10.1039/d4ay02046d","url":null,"abstract":"<p><p>Ribonucleic acid (RNA), essential for protein production and immune function, undergoes glycosylation, a process that attaches glycans to RNA, generating unique glycoRNAs. These glycan-coated RNA molecules regulate immune responses and may be related to immune disorders. However, studying them is challenging due to RNA's fragility. Therefore, a robust method for identifying glycoRNA is important. To address this, we optimized parameters for RNA stability, oxidation, and digestion, thereby enriching and identifying glycoRNAs. This breakthrough paves the way for exploring their potential interactions with immune receptors and tumor suppression. Our approach involved investigating factors such as preservation reagent, enzyme buffer, digestion temperature, oxidant, glycosidase, and incubation time. We successfully optimized digestion conditions, achieving efficient cleavage of <i>N</i>-linked glycoRNAs at room temperature using 25 mM ammonium bicarbonate, demonstrating the effectiveness of this method. Additionally, RNA preservation in RNAlater at -80 °C allows controlled release of glycoRNAs within hours. While sequential digestion of different glycoRNA types is possible, significant degradation occurs after the first glycosidase step. Therefore, we recommend separate harvesting for each glycoRNA type. We also established RNA-seq analysis for identifying various glycoRNA types, including snoRNAs and tRNAs. The optimized SPCgRNA method paves the way for further research on <i>N</i>-glycosylation in health and disease.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"964-971"},"PeriodicalIF":2.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrated magnetoimpedance biosensor microfluidic magnetic platform for the evaluation of the cardiac marker cTnI.","authors":"Zhen Yang, Jingyuan Chen, Mengyu Liu, Jiabao Huang, Jieping Liang, Mengjiao Zhu, Yuanwei Shen, Danqing Li, Chong Lei, Xuecheng Sun","doi":"10.1039/d4ay02021a","DOIUrl":"10.1039/d4ay02021a","url":null,"abstract":"<p><p>An integrated magnetoimpedance (MI) biosensor microfluidic magnetic platform was proposed for the evaluation of the cardiac marker, cardiac troponin I (cTnI). This bioanalyte evaluation platform mainly comprised three external permanent magnets (PMs), one MI element, two peelable SiO₂ film units and a microfluidic chip (MFC). The MI element was made of micro-electro-mechanical system (MEMS)-based multilayered [Ti (6 nm)/FeNi (100 nm)]₅/Cu (400 nm)/[Ti (6 nm)/FeNi (100 nm)]₅ thin films and designed as meander structures with closed magnetic flux. The MFC was fabricated using 3D printing and inverted molding techniques, designed with a solution by mixing the reaction region, magnetic separation region and detection region. Peelable SiO₂ film units with the same size as the MI sensing element were used as the immunoreactivity interface of the bioanalytes. Two large PMs were placed directly below the MI sensing unit to provide a bias magnetic field, and the smaller PM was embedded in MCF for magnetic separation function. Different concentrations of the biological target (cTnI antigen)-, PBS buffer-, and Dynabeads-labeled polyclonal cTnI antibody solution were injected sequentially into the MCF. After immunoreactivity and magnetic separation, a classical sandwich immunoreaction process occurred on the surface of the monoclonal antibody-modified SiO₂ film <i>via</i> self-assembling process in the reaction region of the MFC. The fundamental principle for evaluation of cTnI was based on variations of the MI signal under different concentrations of the biological target coupled with different numbers of Dynabeads. It was demonstrated that the mentioned MI-based magnetic platform could perform quantitative detection analyses over a range of cTnI concentrations (lowest concentration = 0.1 ng mL^-1 and highest concentration = 100 ng mL^-1). The proposed MI-based magnetic platform provides a sensitive, reliable, stable and reusable bioanalytical platform, and it has potential in future biomedical applications.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"990-998"},"PeriodicalIF":2.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142918702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on a molecularly imprinted electrochemical sensor based on a graphene quantum dot-gold nanoparticle composite for the determination of 17β-estradiol.","authors":"Wei Yuan, Yipeng Wang, Meng Jiang, Yan Jin, Wen Yan, Qingyu Wang, Lingmei Niu","doi":"10.1039/d4ay01943a","DOIUrl":"10.1039/d4ay01943a","url":null,"abstract":"<p><p>In this study, a molecularly imprinted electrochemical sensor (MIECS) was constructed based on the combination of graphene quantum dots-gold nanoparticles (GQDs-AuNPs), molecular imprinting polymer (MIP), and electrochemical technology for the ultra-sensitive detection of 17β-estradiol (E₂). GQDs-AuNPs were synthesized and modified on the surface of glassy carbon electrodes (GCE). Safranine T was used as the functional monomer and E₂ was the template molecule for self-assembly and electropolymerization, thus generating an MIP film on the electrode surface. By elution, a large number of recognition sites for E₂ were left in the polymer matrix. Before and after the combination with the target, there is an obvious change in the peak current signal, which enables the quantitative detection of E₂ to be achieved. Under the optimized conditions, the concentration of E₂ showed a good linear relationship with the peak current of the sensor in the range of 1 × 10^-5-1 × 10^-14 M, and the detection limit was 2.2 fM. The molecularly imprinted electrochemical sensor based on GQDs-AuNPs established in this study offers the features of simplicity of operation, low experimental cost, and high sensitivity. This method successfully detected E₂ in milk, urine, and human serum, demonstrating its potential for broad application in clinical practice.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"999-1009"},"PeriodicalIF":2.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142918725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Encapsulation of biomimetic nanozymes in MOF matrices as peroxidase mimetics for sensitive detection of L-cysteine.","authors":"Shaohong Zhang, Ran Zhang, Minzi Li, Hongying Wang, Zhijuan Wang","doi":"10.1039/d4ay01844c","DOIUrl":"10.1039/d4ay01844c","url":null,"abstract":"<p><p>A metal-organic framework (MOF)-encapsulated nanozyme has been developed to detect L-cysteine (L-Cys) through a facile colorimetric sensing method in this study. This nanozyme was prepared by encapsulating Fmoc-histidine (FH) and hemin within ZIF-8 (FH/hemin@ZIF-8) and exhibited significantly enhanced catalytic activity and great stability because of its unique structure. FH/hemin@ZIF-8 oxidized colorless 3,3',5,5'-tetramethylbenzidine (TMB) to a distinct blue color with the assistance of H₂O₂. However, after the addition of L-Cys, this oxidation process was inhibited, resulting in the solution fading from blue to colorless. This change can be observed by the naked eyes and quantitatively analyzed using a UV-vis spectrophotometer. Additionally, this system demonstrated excellent resistance to interference and exceptional selectivity. In addition, this system showed a low detection limit of 23.1 nm (S/N = 3). Consequently, it is believed that the strategy of encapsulating biomimetic nanozymes within MOFs holds significant potential for applications in bioanalysis and the early diagnosis of L-Cys.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"955-963"},"PeriodicalIF":2.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142929937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel fluorescent probe for rapid and selective detection of fluoride ions in living cells.","authors":"Tingting Feng, Jiaxue Yang, Yi Wang, Taozhu Hu, Longjia Yan, Yi Le, Li Liu","doi":"10.1039/d4ay02173h","DOIUrl":"10.1039/d4ay02173h","url":null,"abstract":"<p><p>A novel fluorescent probe DTP, based on fluorine-silicon complexation, extends emission to 590 nm and achieves a 5 minutes response time. It shows high selectivity and a 0.98 μM detection limit for fluoride ions, with successful bioimaging application in living cells.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"939-943"},"PeriodicalIF":2.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-cell NMR reveals metabolic adaptations in central carbon pathways driving antibiotic tolerance in <i>Salmonella</i> Typhimurium.","authors":"Alexandra L N Zahid, Ke-Chuan Wang, Line Elnif Thomsen, Sebastian Meier, Pernille Rose Jensen","doi":"10.1039/d4ay02023e","DOIUrl":"10.1039/d4ay02023e","url":null,"abstract":"<p><p>Antibiotic tolerance presents a significant challenge in eradicating bacterial infections, as tolerant strains can survive antibiotic treatment, contributing to the recurrence of infections and the development of resistance. However, unlike antibiotic resistance, tolerance is not detectable by standard susceptibility assays such as minimal inhibitory concentration (MIC) tests. Consequently, antibiotic tolerance often goes unnoticed in clinical settings. Bacterial metabolism is closely linked to antibiotic efficacy, and thus presents as a potential target for novel diagnostic methods. Recent advancements in nuclear magnetic resonance (NMR) spectroscopy, including dynamic nuclear polarization (DNP-NMR), enable a non-invasive real-time approach to analyzing bacterial metabolism. In this study, we applied both ¹H and in-cell ¹³C NMR spectroscopy to investigate metabolic adaptations in a tolerance-evolved <i>Salmonella</i> Typhimurium strain, C10, developed through ten cycles of ampicillin treatment. Our results demonstrated that despite similar MICs and growth rates, the C10 strain exhibited a 25-fold increase in tolerance compared to the wild-type, while exhibiting lower metabolic activity. Under ampicillin stress, however, the C10 strain maintained higher metabolic activity and demonstrated greater resilience in glucose consumption and metabolite production relative to the wild-type. Using DNP-NMR, rapid metabolic shifts in the C10 strain were identified within 10 minutes of exposure to high concentrations of ampicillin, characterized by accumulation of key metabolites such as pyruvate and acetate. Overall, our findings underscore the potential of real-time NMR-based analyses to provide deeper insights into antibiotic tolerance and distinguish between susceptible and tolerant bacterial strains.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"1062-1073"},"PeriodicalIF":2.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facile preparation of a hydrophilic Eu-based ratiometric fluorescent nanosensor for Cu²⁺ ion detection and imaging in living cells.","authors":"Moru Zhang, Chunyun Deng, Jing Chen, Shucai Liang","doi":"10.1039/d4ay01984a","DOIUrl":"10.1039/d4ay01984a","url":null,"abstract":"<p><p>In this work, a hydrophilic Eu-based ratiometric fluorescent nanosensor (PAAC-Eu) was developed for Cu²⁺ ion detection in aqueous solutions and imaging in living cells. The sensor was prepared <i>via</i> a simple one-step reaction at room temperature, leveraging the synergistic coordination of commercially accessible polyacrylic acid (PAA) and coumarin-3-carboxylic acid (CCAH) with Eu³⁺ ions. PAAC-Eu was easy to disperse in aqueous media and exhibited two characteristic emission bands at 406 nm and 618 nm, respectively, upon excitation at 350 nm. Cu²⁺ ions could bind with the free carboxyl groups in PAAC-Eu within 10 min, leading to a decrease in fluorescence at 618 nm (<i>I</i>₆₁₈) and a negligible effect on fluorescence at 406 nm (<i>I</i>₄₀₆). Accordingly, a rapid, sensitive and selective method for detecting Cu²⁺ ions was established, which could complete Cu²⁺ ion assay within 30 min. A good linear relationship was observed between <i>I</i>₄₀₆/<i>I</i>₆₁₈ and Cu²⁺ ion concentration at 0-20.0 μM (0-1.28 mg L^-1) with a detection limit as low as 0.175 μM (11.2 μg L^-1). The proposed method was successfully applied to quantify Cu²⁺ ions in real water samples. Moreover, a portable paper-based sensor was developed by loading PAAC-Eu in filter paper, which could enable visual detection of Cu²⁺ ions without using large instruments. Finally, cell experiments demonstrated the low cytotoxicity and good cell permeability of PAAC-Eu, and ratiometric fluorescence imaging of Cu²⁺ ions in living cells was successfully performed.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"944-954"},"PeriodicalIF":2.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new stability indicating HPLC method with QDa and PDA detectors for the determination of process and degradation impurities of ivabradine including separation of diastereomeric <i>N</i>-oxides.","authors":"Büşra Gülşen, Sıdıka Ertürk Toker","doi":"10.1039/d4ay01986e","DOIUrl":"10.1039/d4ay01986e","url":null,"abstract":"<p><p>In this study, a new reversed phase high performance liquid chromatography method using two detectors was developed for the analysis of degradation and process impurities of ivabradine in pharmaceutical preparations. A PDA detector set to 285 nm wavelength and a QDa detector set to positive scan mode were used in the method. In the developed method, the separation process was carried out in a Zorbax phenyl column with a gradient application of a 0.075% trifluoroacetic acid, acetonitrile, and methanol mixture at a flow rate of 1.5 ml min^-1. During the degradation studies, the samples were exposed to acidic, alkaline, oxidative, thermal, and photolytic conditions. Process-related impurities were separated not only without interfering with each other but also with the degradation product and ivabradine peaks, and thanks to QDa, all impurities could be identified with their molecular weights. This method, in addition to providing stability data, was also able to separate two diastereomeric <i>N</i>-oxide impurities which are major oxidative degradation impurities of ivabradine having a chiral center. Some additional measurements such as solubility, specific rotation, melting point and differential scanning calorimetry analysis proved the formation of the two diastereomeric <i>N</i>-oxide impurities under oxidative conditions. Method validation was performed according to the International Council for Harmonization guidelines and the analysis of ivabradine, its process related impurities (dehydro ivabradine, acetyl ivabradine, and hydroxy ivabradine) and a major oxidative degradation product (ivabradine <i>N</i>-oxide) was successfully performed by this method.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"1111-1124"},"PeriodicalIF":2.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}