Analytical Methods最新文献

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Analysis of alkyl-anthraquinone derivatives from hydrogen peroxide industrial process, using LC × LC-HRMS with shifting gradients.
IF 2.7 3区 化学
Analytical Methods Pub Date : 2024-12-19 DOI: 10.1039/d4ay01905a
Clémence Giffard, Florent Rouvière, Olivier Falletti, Béatrice Allard-Breton, Laurent Wendlinger, Jean-Michel Bossoutrot, Karine Faure
{"title":"Analysis of alkyl-anthraquinone derivatives from hydrogen peroxide industrial process, using LC × LC-HRMS with shifting gradients.","authors":"Clémence Giffard, Florent Rouvière, Olivier Falletti, Béatrice Allard-Breton, Laurent Wendlinger, Jean-Michel Bossoutrot, Karine Faure","doi":"10.1039/d4ay01905a","DOIUrl":"https://doi.org/10.1039/d4ay01905a","url":null,"abstract":"<p><p>The characterization of industrial working solutions containing numerous structurally related compounds and isomers requires the use of two-dimensional liquid chromatography coupled with high-resolution mass spectrometry (LC × LC-HRMS). The separation of alkyl-anthraquinone derivatives produced during hydrogen peroxide production was achieved by coupling a biphenyl and a C18 in the first and second dimensions, combined with the use of continuous shifting gradients in the second dimension (<sup>2</sup>D). The use of shifting gradients offers a significant advantage over regular gradients, with a 20% increase in occupancy and better separation of isomers eluted within the same modulation. Additionally, MS spectra were improved by reducing ion suppression in the source. The analysis of the industrial solutions produced a list of 226 peaks with 75 different molar masses, thereby confirming the presence of many isomers. The excellent repeatability of the retention times (within ±0.28 s in the second dimension) enabled the method to be used to study the differences between production batches, which were divided into two groups according to their measured hydrogen peroxide yield. Using multivariate statistical tools on the area of each peak, a total of 191 variables, including 11 clusters of insufficiently resolved isomers, were studied by principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). A simple Wilcoxon test performed on the most pertinent variables was able to confirm that 28 peaks could be used to monitor the industrial process. This work highlighted the efficacy of combining a very powerful separation technique with the implementation of two simple statistical tests to rapidly identity a set of discriminant markers in a highly complex industrial mixture.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A polyaniline-enhanced quartz crystal microbalance sensor for room-temperature camphor detection.
IF 2.7 3区 化学
Analytical Methods Pub Date : 2024-12-18 DOI: 10.1039/d4ay01859a
Rizky Aflaha, Muammar Romiz Dzaki, Laila Katriani, Ahmad Hasan As'ari, Chlara Naren Maharani, Agus Kuncaka, Taufik Abdillah Natsir, Aditya Rianjanu, Ruchi Gupta, Kuwat Triyana, Roto Roto
{"title":"A polyaniline-enhanced quartz crystal microbalance sensor for room-temperature camphor detection.","authors":"Rizky Aflaha, Muammar Romiz Dzaki, Laila Katriani, Ahmad Hasan As'ari, Chlara Naren Maharani, Agus Kuncaka, Taufik Abdillah Natsir, Aditya Rianjanu, Ruchi Gupta, Kuwat Triyana, Roto Roto","doi":"10.1039/d4ay01859a","DOIUrl":"https://doi.org/10.1039/d4ay01859a","url":null,"abstract":"<p><p>A method to detect camphor gas is considered indispensable in the pharmaceutical industry. Unfortunately, the available sensors to detect the presence of camphor in the air are very limited and still on a laboratory scale, such as using chromatography-mass spectroscopy (GC-MS). The research's main focus is to obtain a portable sensing system with excellent sensitivity and selectivity. This study explored polyaniline (PANi) concentrations cast over PVAc nanofiber as a matrix to detect camphor gas using a quartz crystal microbalance (QCM) system to measure camphor exposure. Scanning electron microscopy (SEM) and Fourier-transform infrared (FTIR) were used to analyze the morphology and chemical composition of the fabricated active layer (<i>i.e.</i>, nanofiber with PANi thin film). Increasing the PANi concentration provides more PANi on the sensor surface, thus amassing the active groups to interact with camphor gas molecules. It shows that a sensor with a 0.08% PANi thin film (Nano-PANi8) has a sensitivity of 2.594 Hz ppm<sup>-1</sup>, much greater than the sensor without PANi, which is only 0.305 Hz ppm<sup>-1</sup>. In addition, the sensor also has good repeatability and rapid response and recovery time of 47 s and 133 s, respectively. Compared to other gaseous compounds, the sensor also has excellent selectivity for camphor and robust long-term stability over three weeks of testing. The produced QCM sensor employing PANi thin film can give a camphor sensor superior performance, including excellent sensitivity, selectivity, and long-term stability. Furthermore, the use of QCM as a base sensor also makes the fabricated sensor portable.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Self-reduction of gold@platinum bimetallic nanoparticles on Ti3C2Tx MXene nanoribbons coupled with hydrogel and smartphone technology for colorimetric detection of silver ions.
IF 2.7 3区 化学
Analytical Methods Pub Date : 2024-12-18 DOI: 10.1039/d4ay01907e
Jing Li, Xun Zhou, Guanghua Mao, Gangbing Zhu, Yinhui Yi
{"title":"Self-reduction of gold@platinum bimetallic nanoparticles on Ti<sub>3</sub>C<sub>2</sub>T<sub><i>x</i></sub> MXene nanoribbons coupled with hydrogel and smartphone technology for colorimetric detection of silver ions.","authors":"Jing Li, Xun Zhou, Guanghua Mao, Gangbing Zhu, Yinhui Yi","doi":"10.1039/d4ay01907e","DOIUrl":"https://doi.org/10.1039/d4ay01907e","url":null,"abstract":"<p><p>In recent years, numerous colorimetric methods have been developed for the detection of silver ions (Ag<sup>+</sup>), yet there remains a need for a simple, sensitive, real-time and quantitative sensing platform. Herein, Ti<sub>3</sub>C<sub>2</sub>T<sub><i>x</i></sub> MXene nanoribbons (Ti<sub>3</sub>C<sub>2</sub>T<sub><i>x</i></sub>NRs) were utilized as the carrier material, and gold@platinum (Au@Pt) bimetallic nanoparticles were decorated onto the Ti<sub>3</sub>C<sub>2</sub>T<sub><i>x</i></sub>NR surface, for the first time, <i>via</i> a facile self-reduction method. The resulting Au@Pt-Ti<sub>3</sub>C<sub>2</sub>T<sub><i>x</i></sub>NR nanohybrid exhibited excellent catalytic activity, facilitating the oxidation of 3,3',5,5'-tetramethylbenzidine, a colorless substrate, to generate a blue product (oxTMB), displaying prominent peroxidase-like activity. In the presence of Ag<sup>+</sup>, a remarkable inhibiting effect was observed on the catalytic activity of the Au@Pt-Ti<sub>3</sub>C<sub>2</sub>T<sub><i>x</i></sub>NR nanohybrids, effectively halting the generation of oxTMB. Based on this, the as-obtained Au@Pt-Ti<sub>3</sub>C<sub>2</sub>T<sub><i>x</i></sub>NR nanozyme was then utilized to develop a novel colorimetric sensing platform for Ag<sup>+</sup> detection, with a low detection limit of 1.57 nM and a wide linear detection range from 5.0 nM to 9.0 μM. In addition, by combining the unique advantages of hydrogel materials and smartphone technology, a simple, real-time and quantitative platform for Ag<sup>+</sup> monitoring was constructed, highlighting its potential for practical applications in Ag<sup>+</sup> detection.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An electrochemical sensor based on electrodeposited methylene blue on a carbon nanotube decorated hydrogel for the detection of ascorbic acid.
IF 2.7 3区 化学
Analytical Methods Pub Date : 2024-12-18 DOI: 10.1039/d4ay01827c
Linghui Tang, Yufeng Huang, Zhihuan Qian, Jifan Zhao, Yasushi Hasebe, Yan Dong, Yue Wang
{"title":"An electrochemical sensor based on electrodeposited methylene blue on a carbon nanotube decorated hydrogel for the detection of ascorbic acid.","authors":"Linghui Tang, Yufeng Huang, Zhihuan Qian, Jifan Zhao, Yasushi Hasebe, Yan Dong, Yue Wang","doi":"10.1039/d4ay01827c","DOIUrl":"https://doi.org/10.1039/d4ay01827c","url":null,"abstract":"<p><p>In this study, a self-assembled electrochemical sensor was prepared by coating with a carbon nanotube (CNT) decorated hydrogel (HG) combined with electrodeposition of methylene blue (MB), and then used for the detection of ascorbic acid (AA). The three-dimensional network of HG has the advantages of large electroactive surface area, rapid diffusion and electron transfer rate, strong adhesive ability and stabilization of the polymerized MB. The MB provides high electrocatalytic activity and works as an electron transfer mediator to facilitate the oxidation of AA. The successful synthesis of the hydrogel and the preparation of the sensor are confirmed by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS). The layer-by-layer assembly was identified by AFM with the heights of 22.1 nm and 186.8 nm for the hydrogel and MB layers, respectively. Under the optimal conditions, the sensor has a linear range of 0.1 mM to 10 mM and a detection limit of 0.05 mM. What's more, the prepared sensor also exhibits good stability (current retention of 91.22% after 100 cycles for testing 0.25 mM AA), excellent anti-interference ability, good reproducibility (RSD of 4.26% for five independent experiments), excellent operational stability (RSD of 1.66% for 30 consecutive AA additions), fast response time (<4 s) and shows satisfactory results in the detection of AA in vitamin C tablets.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Localized surface plasmon resonance sensing based on monometallic gold nanoparticles: from material preparation to detection of bioanalytes.
IF 2.7 3区 化学
Analytical Methods Pub Date : 2024-12-18 DOI: 10.1039/d4ay01509f
Peng Zhang, Huizhen Hou, Songshi Xu, Yingfei Wen, Yonghui Zhang, Fei Xing
{"title":"Localized surface plasmon resonance sensing based on monometallic gold nanoparticles: from material preparation to detection of bioanalytes.","authors":"Peng Zhang, Huizhen Hou, Songshi Xu, Yingfei Wen, Yonghui Zhang, Fei Xing","doi":"10.1039/d4ay01509f","DOIUrl":"https://doi.org/10.1039/d4ay01509f","url":null,"abstract":"<p><p>The tunable geometrical properties of gold nanoparticles (AuNPs) endow them with the capacity to exhibit distinct behaviors with respect to both macroscopic (color) and microscopic (resonance wavelength) aspects, which has been extensively utilized in localized surface plasmon resonance (LSPR) sensing platforms. Additionally, functionalizing AuNP surfaces enhances the platforms' capabilities, allowing for the detection of a wide range of molecules related to various aspects of human health. In this review, we comprehensively elucidate the fundamental principles of LSPR biosensing and provide an in-depth survey of the preparation processes for metal nanoparticles, encompassing deposition technology for large-scale particle production as well as ion reduction methods that afford superior control over the particles' physical and chemical attributes. The sensing strategies based on adjustment of the dielectric environment and particle dispersion-aggregation levels are thoroughly reviewed and discussed. The discussion focused on a specific class of nanoparticles, characterized by their uniform shape and size, with each section bifurcated into two parts: a summary of the salient features and recent discoveries pertaining to the sensing strategy, as well as illustrations of representative, cutting-edge applications employing the strategy. We specifically aim to scrutinize analytes commonly encountered in the biomedical realm, encompassing biomarkers that serve as indicators of a wide range of diseases and microbial pathogens, while also prognosticating the future development trends of LSPR optical biosensor platforms within the biomedical field.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An interpretable multi-scale convolutional attention residual neural network for glioma grading with Raman spectroscopy. 利用拉曼光谱对胶质瘤分级的可解释多尺度卷积注意残差神经网络。
IF 2.7 3区 化学
Analytical Methods Pub Date : 2024-12-17 DOI: 10.1039/d4ay02068e
Qingbo Li, Xupeng Shao, Yan Zhou, Yinyan Wang, Zeya Yan, Hongbo Bao, Lipu Zhou
{"title":"An interpretable multi-scale convolutional attention residual neural network for glioma grading with Raman spectroscopy.","authors":"Qingbo Li, Xupeng Shao, Yan Zhou, Yinyan Wang, Zeya Yan, Hongbo Bao, Lipu Zhou","doi":"10.1039/d4ay02068e","DOIUrl":"https://doi.org/10.1039/d4ay02068e","url":null,"abstract":"<p><p>Since the malignancy of gliomas varies with their grade, classifying gliomas of different grades can assist doctors in developing personalized surgical plans during surgery, thereby improving the prognosis. Raman spectroscopy is an optical method for real-time glioma diagnosis. However, high-grade glioma (HGG, WHO grades III and IV), low-grade glioma (LGG, WHO grades I and II) and normal tissue have similar biochemical components, leading to similar spectral characteristics. This similarity reduces classification accuracy when using traditional machine learning methods. In contrast, deep learning offers enhanced feature extraction capabilities without the need for extensive feature engineering. Nevertheless, the diversity in the scale of spectral features presents challenges in designing a neural network that effectively adapts to these characteristics. To address these issues, this paper proposes a Multi-Scale Convolutional Attention Residual Network (M-SCA ResNet), which incorporates multi-scale channel and spatial attention mechanisms along with residual structures to improve the model's feature extraction capabilities. The algorithm presented in this study, was employed to classify HGG, LGG, and healthy tissue and was compared with conventional machine learning and neural networks. The results indicate that the M-SCA ResNet achieved an identification accuracy exceeding 85% for all three tissue types, along with the highest weighted <i>F</i><sub>1</sub>-score. Furthermore, to enhance the interpretability of deep learning models, Gradient-weighted Class Activation Mapping (Grad-CAM) was utilized to extract and visualize key Raman shifts that significantly contribute to classification. Most of the extracted Raman shifts correspond to characteristic peaks of brain tissue which have been demonstrated to be effective in distinguishing between glioma of different grades and normal tissue in previous studies. This finding proves the strong correlation between the feature extraction capabilities of the M-SCA ResNet and the biomolecular characteristics of various tissues. The experiments conducted in this study validate the feasibility of using the M-SCA ResNet for glioma grading and provide valuable support for formulating subsequent surgical and treatment plans, indicating its promising application in <i>in vivo</i> and <i>in situ</i> spectral diagnosis of glioma grading.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A DNA conformational nanoswitch for amplification of both low-abundance protein imaging in living cells and photodynamic therapy.
IF 2.7 3区 化学
Analytical Methods Pub Date : 2024-12-17 DOI: 10.1039/d4ay02053g
Mingzhu Fan, Wei Guo, Xinran Li, Huile Fan, Zhihui Luo, Jiayao Xu, Huakui Huang, Shulong Wang
{"title":"A DNA conformational nanoswitch for amplification of both low-abundance protein imaging in living cells and photodynamic therapy.","authors":"Mingzhu Fan, Wei Guo, Xinran Li, Huile Fan, Zhihui Luo, Jiayao Xu, Huakui Huang, Shulong Wang","doi":"10.1039/d4ay02053g","DOIUrl":"https://doi.org/10.1039/d4ay02053g","url":null,"abstract":"<p><p>A method was developed for inducing a DNA conformational nanoswitch triggered by proteins, intended for fluorescence signal amplification imaging and photodynamic therapy targeting tumor cells.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recent developments in isothermal amplification technology for rapid detection of SARS-CoV-2. 用于快速检测 SARS-CoV-2 的等温扩增技术的最新发展。
IF 2.7 3区 化学
Analytical Methods Pub Date : 2024-12-16 DOI: 10.1039/d4ay01106f
Linlin Wang, Ruitong Tang, Wentao Wang, Lingguang Bu, Jingle Sun, Yurong Fu, Meng Li, Zhengjun Yi
{"title":"Recent developments in isothermal amplification technology for rapid detection of SARS-CoV-2.","authors":"Linlin Wang, Ruitong Tang, Wentao Wang, Lingguang Bu, Jingle Sun, Yurong Fu, Meng Li, Zhengjun Yi","doi":"10.1039/d4ay01106f","DOIUrl":"https://doi.org/10.1039/d4ay01106f","url":null,"abstract":"<p><p>Coronavirus disease 2019 (COVID-19), an infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has spread globally, posing a significant threat to human health. Rapid and accurate detection of infectious disease pathogens is of crucial practical significance for early screening, timely intervention, and outbreak prevention. However, conventional diagnostic methods are increasingly unable to meet clinical demands. Recently developed isothermal analysis methods offer mild reaction conditions and reduce dependence on specialized instruments. These convenient, fast, and reliable methods show great promise for diagnosing infectious pathogens, especially for on-site detection in areas without laboratories or with limited resources. Among them, loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA), which integrate various widely used detection techniques, stand out as rapidly advancing and relatively mature isothermal nucleic acid amplification technologies. This review outlines several representative isothermal amplification technologies and associated detection methods. We summarize the latest advancements in LAMP and RPA technologies for the rapid detection of SARS-CoV-2 and discuss the future prospects of isothermal amplification in diversified testing.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142826632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quantitative and rapid lateral flow immunoassay for cardiac troponin I using dendritic mesoporous silica nanoparticles and gold nanoparticles. 使用树枝状介孔二氧化硅纳米颗粒和金纳米颗粒对心肌肌钙蛋白 I 进行定量和快速横向流动免疫测定。
IF 2.7 3区 化学
Analytical Methods Pub Date : 2024-12-16 DOI: 10.1039/d4ay02060j
Yafei Li, Yu Yao, Qingqing Hua, Jishun Li
{"title":"Quantitative and rapid lateral flow immunoassay for cardiac troponin I using dendritic mesoporous silica nanoparticles and gold nanoparticles.","authors":"Yafei Li, Yu Yao, Qingqing Hua, Jishun Li","doi":"10.1039/d4ay02060j","DOIUrl":"https://doi.org/10.1039/d4ay02060j","url":null,"abstract":"<p><p>Acute myocardial infarction (AMI) is considered to be one of the predominant causes of human death; therefore, a rapid and accurate diagnostic method for AMI is urgently required. In this work, a highly sensitive lateral flow immunoassay (LFIA) platform was designed and fabricated for the quantitative determination of cardiac troponin I (cTnI) using a scanner, a smartphone and a colloidal gold immunoassay analyzer. To overcome the limitation of low sensitivity of traditional colloidal gold-based LFIA, three-dimensionally assembled gold nanoparticles (AuNPs) within a dendritic mesoporous silica nanoparticle (DMSN) scaffold were fabricated as signal labels. The assembly structure greatly enhanced the light extinction ability of a single label for signal amplification. The DMSNs@Au-based LFIA strips exhibited excellent detection performance including a high sensitivity (LOD = 70 pg mL<sup>-1</sup>) and wide linear range (0.5-40 ng mL<sup>-1</sup>) and precision with good specificity. The successful determination of cTnI by the test strips provides the ability to diagnose AMI at an early stage and expands the diagnostic window of AMI, while also having advantages such as low cost and user-friendliness. Therefore, we believe that the test strips fabricated in this work have great potential to be applied for practical clinical applications for the early and accurate diagnosis of AMI.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142826628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multiplexed centrifugal microfluidic system for rapid and non-invasive detection of myocardial fibrosis. 用于快速无创检测心肌纤维化的多重离心微流控系统。
IF 2.7 3区 化学
Analytical Methods Pub Date : 2024-12-16 DOI: 10.1039/d4ay01703j
Luhai Wang, Jiaze Sun, Siwei Dai, Pengfei Zhang, Yefei Zhu, Yu Zhang
{"title":"Multiplexed centrifugal microfluidic system for rapid and non-invasive detection of myocardial fibrosis.","authors":"Luhai Wang, Jiaze Sun, Siwei Dai, Pengfei Zhang, Yefei Zhu, Yu Zhang","doi":"10.1039/d4ay01703j","DOIUrl":"https://doi.org/10.1039/d4ay01703j","url":null,"abstract":"<p><p>Myocardial fibrosis is a pathological condition characterized by the excessive accumulation of extracellular matrix proteins, primarily collagen, within the myocardium. Early symptoms of myocardial fibrosis are often subtle, leading to late detection. As the disease progresses, it is often associated with life-threatening complications. Therefore, myocardial fibrosis urgently demands a diagnostic system that is rapid, effective, and preferably non-invasive. In this study, we developed a highly sensitive microfluidic light-initiated chemiluminescent assay (LICA) for the simultaneous detection of cTnI, NT-proBNP, HA, LN, PICP, and PIIINP, the key biomarkers involved in the occurrence and development of myocardial fibrosis. The system is highly integrated and compact, incorporating reagent lyophilization and whole blood separation technologies, and is suitable for point-of-care testing. By combining advantages of centrifugal microfluidics and LICA, the system effectively shortens the detection time for key biomarkers associated with MACEs. Moreover, the system provides optimal analytical performance, with LoDs of 0.0018 ng mL<sup>-1</sup>, 0.0127 ng mL<sup>-1</sup>, 3.52 ng mL<sup>-1</sup>, 6.88 ng mL<sup>-1</sup>, 4.68 ng mL<sup>-1</sup>, and 0.072 ng mL<sup>-1</sup> for cTnI, NT-proBNP, HA, LN, PIIINP and PICP, respectively. Ultimately, the system can achieve an AUC of 0.548, 0.670, 0.772, 0.752, 0.833 and 0.713 for cTnI, NT-proBNP, HA, LN, PIIINP, and PICP, respectively, and an overall combined ROC of 0.946 for the detection of myocardial fibrosis, indicating its excellent efficacy and potential for widespread clinical applications.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142826626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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