Analytical Methods最新文献_第3页

Turn-on fluorescent probe based on dicyanoisophorone for bioimaging and rapid detection of peroxynitrite in aqueous media. 基于二氰异佛尔酮的开启式荧光探针，用于生物成像和水介质中过氧亚硝酸盐的快速检测。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-11-07 DOI: 10.1039/d4ay01721h

Jianwei Wu, Jia Li, Yaqiao Shi, Liting Jiang, Chenming Chan, Ru Feng, Yue Wang, Zhaoli Xue

引用次数: 0

New perspective on central nervous system disorders: focus on mass spectrometry imaging. 中枢神经系统疾病的新视角：聚焦质谱成像。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-11-07 DOI: 10.1039/d4ay01205d

Huaqing Lai, Pinglong Fan, Huiqin Wang, Zhenzhen Wang, Naihong Chen

{"title":"New perspective on central nervous system disorders: focus on mass spectrometry imaging.","authors":"Huaqing Lai, Pinglong Fan, Huiqin Wang, Zhenzhen Wang, Naihong Chen","doi":"10.1039/d4ay01205d","DOIUrl":"https://doi.org/10.1039/d4ay01205d","url":null,"abstract":"An abnormally organized brain spatial network is linked to the development of various central nervous system (CNS) disorders, including neurodegenerative diseases and neuropsychiatric disorders. However, the complicated molecular mechanisms of these diseases remain unresolved, making the development of treatment strategies difficult. A novel molecular imaging technique, called mass spectrometry imaging (MSI), captures molecular information on the surface of samples in situ. With MSI, multiple compounds can be simultaneously visualized in a single experiment. The high spatial resolution enables the simultaneous visualization of the spatial distribution and relative content of various compounds. The wide application of MSI in biomedicine has facilitated extensive studies on CNS disorders in recent years. This review provides a concise overview of the processes, applications, advantages, and disadvantages, as well as mechanisms of the main types of MSI. Meanwhile, this review summarizes the main applications of MSI in studying CNS diseases, including Alzheimer's disease (AD), CNS tumors, stroke, depression, Huntington's disease (HD), and Parkinson's disease (PD). Finally, this review comprehensively discusses the synergistic application of MSI with other advanced imaging modalities, its utilization in organoid models, its integration with spatial omics techniques, and provides an outlook on its future potential in single-cell analysis.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A dual-functional rhodamine B and azo-salicylaldehyde derivative for the simultaneous detection of copper and hypochlorite: synthesis, biological applications and theoretical insights. 用于同时检测铜和次氯酸盐的双功能罗丹明 B 和偶氮水杨醛衍生物：合成、生物应用和理论见解。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-11-07 DOI: 10.1039/d4ay01758g

Vishnu S, Avijit Kumar Das, Yatheesharadhya Bylappa, Anish Nag, Malay Dolai

{"title":"A dual-functional rhodamine B and azo-salicylaldehyde derivative for the simultaneous detection of copper and hypochlorite: synthesis, biological applications and theoretical insights.","authors":"Vishnu S, Avijit Kumar Das, Yatheesharadhya Bylappa, Anish Nag, Malay Dolai","doi":"10.1039/d4ay01758g","DOIUrl":"https://doi.org/10.1039/d4ay01758g","url":null,"abstract":"A multifunctional rhodamine derivative containing azo-salicylaldehyde (BBS) was designed and synthesized as a colorimetric and fluorescence turn-on probe for the selective detection of copper cations (Cu2+) and hypochlorite anions (OCl-) in aqueous media. In the presence of Cu2+, the probe BBS exhibited turn-on absorption and fluorescence change at 554 nm and 585 nm, respectively. The binding mechanism of BBS with Cu2+ induces the opening of a spirolactam ring in the rhodamine moiety by the formation of a metal-ligand complex, achieving 10-fold enhancement in fluorescence and quantum yield, along with a binding constant of 1 × 104 M-1 and a detection limit of 2.61 μM. Addition of OCl- enhanced the absorbance and fluorescence intensities at 520 nm and 575 nm, respectively. The probe BBS underwent hypochlorite-mediated oxidation, followed by hydrolysis, resulting in the formation of rhodamine B itself, which is detectable by the naked eye via the color and fluorescence enhancement by 11-fold with a high quantum yield and a detection limit of 1.96 μM. For practical applications, sensor BBS can be used to detect Cu2+ in water samples and on cotton swabs. For biological applications, the interaction of the BBS-Cu(II) complex with transport proteins such as bovine serum albumin (BSA) and ct-DNA was investigated through UV-vis and fluorescence titration experiments. Additionally, the structural optimization of BBS and the BBS-Cu(II) complex was demonstrated using DFT, and the interactions of the BBS-Cu(II) complex with BSA and ct-DNA were analysed through theoretical docking studies. Bioimaging studies were conducted by capturing fluorescence images of BBS with Cu2+ and OCl- in a physiological medium containing living plant tissue using green gram seeds.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Promotion of a quality standard for Scutellariae radix based on the anti-inflammatory efficacy-oriented quality marker of the effect-constituent index. 根据以抗炎功效为导向的功效成分指数质量指标，推广黄芩的质量标准。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-11-07 DOI: 10.1039/d4ay01119h

Dan-Ni Chen, Qiao Liu, Qian-Qian Xue, Ya-Qian Zhou, Miao-Miao Wang, Hui-Xin Liu, Xun Liu, Fang-Zhou Yin

{"title":"Promotion of a quality standard for Scutellariae radix based on the anti-inflammatory efficacy-oriented quality marker of the effect-constituent index.","authors":"Dan-Ni Chen, Qiao Liu, Qian-Qian Xue, Ya-Qian Zhou, Miao-Miao Wang, Hui-Xin Liu, Xun Liu, Fang-Zhou Yin","doi":"10.1039/d4ay01119h","DOIUrl":"10.1039/d4ay01119h","url":null,"abstract":"The quality control of herbal medicines is key to their clinical efficacy. The multi-component and multi-effect characteristics of herbal medicines have prompted scholars to clarify various factors related to quality evaluation through various methods. Nevertheless, the relationship between chemical properties and their associated clinical efficacy is little reflected in the quality control techniques currently in use. To address the issue, a novel herbal quality standard system based on the efficacy-oriented Q-marker of the effect-constituent index (ECI) is promoted in this study, using Scutellariae Radix (SR), a widely used herbal medicine with anti-inflammation, anti-tumor, anti-viral and other therapeutic effects, as a case study. Combined with chromatographic analysis and bioassay, four Q-markers including baicalin, baicalein, wogonin and oroxylin A were selected based on the anti-inflammatory efficacy of SR. The ECI model of SR was constructed by combining the content determination of the Q-markers via ultra-high-performance liquid chromatography-triple quadrupole mass spectroscopy (UHPLC-QqQ-MS/MS) with the corresponding biological potency obtained from the anti-inflammatory effects on tumor necrosis factor (TNF)-α and interleukin (IL)-6 production. Correlation analysis showed that the ECI was significantly correlated with the measured anti-inflammatory activity (p < 0.01). The ECI exhibited a good ability to determine and predict the bioeffect-based quality grade for SR. Overall, the construction and application of the ECI for SR in this study provides a beneficial reference for quality evaluation methods of other herbs with distinct effects and active ingredients.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142589571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A dual-mode sensing platform for electron spin resonance and UV-vis detection of alkaline phosphatase based on Cu-based metal-organic frameworks. 基于铜基金属有机框架的碱性磷酸酶电子自旋共振和紫外-可见检测双模式传感平台。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-11-06 DOI: 10.1039/d4ay01682c

Hui Shi, Yuntong Liu, Chu Qiu, Chunyu Wang, Zhimin Zhang, Meijun Lu, Bo Wang, Yuan Tian, Daqian Song, Ziwei Zhang

{"title":"A dual-mode sensing platform for electron spin resonance and UV-vis detection of alkaline phosphatase based on Cu-based metal-organic frameworks.","authors":"Hui Shi, Yuntong Liu, Chu Qiu, Chunyu Wang, Zhimin Zhang, Meijun Lu, Bo Wang, Yuan Tian, Daqian Song, Ziwei Zhang","doi":"10.1039/d4ay01682c","DOIUrl":"https://doi.org/10.1039/d4ay01682c","url":null,"abstract":"Alkaline phosphatase (ALP) is an indispensable hydrolase in living organisms and the abnormality of ALP activity is correlated with a variety of diseases. Exploring ALP activity is important for clinical diagnosis and biomedical research to understand its physiological function. In this study, a dual-mode biosensing platform was constructed based on Cu-based metal-organic frameworks (Cu-MOFs) for electron spin resonance (ESR) and ultraviolet-visible (UV-vis) sensing of ALP. Cu-MOFs, as peroxidase mimics, catalyzed the decomposition of hydrogen peroxide (H2O2) and the generation of reactive oxygen species (ROS) which could oxidize ABTS into ABTS˙+ with good ESR and UV-vis signals. Pyrophosphate ions (PPi) with high affinity to Cu2+ in Cu-MOFs could suppress the peroxidase-like activity of Cu-MOFs, and ALP could hydrolyze PPi, resulting in the recovery of Cu-MOF catalytic activity. Thus, a quantitative dual-mode method for detection of ALP activity was established with good linearity in the range of 0-42 U L-1 and limits of detection as low as 0.386 and 0.523 U L-1 respectively for ESR and UV-vis detection. Benefiting from its high sensitivity and excellent selectivity, this method was applied for ALP detection in human serum and satisfactory recoveries were achieved. The off-on dual-mode sensing platform is more reliable than the single-mode sensor and shows merits like simple operation and cost-friendliness, making it have great potential in the diagnosis of ALP-related diseases.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A broad-spectrum peptide screening method using an optimized solid-phase extraction and liquid chromatography-high-field asymmetric ion mobility spectrometry-mass spectrometry for doping control in equine urine. 利用优化的固相萃取和液相色谱-高场非对称离子迁移谱-质谱法筛查马尿中兴奋剂的广谱多肽方法。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-11-06 DOI: 10.1039/d4ay01477d

Kohei Ohnuma, Misato Hirano-Kodaira, Michiko Bannai, Yoshibumi Shimizu, Masayuki Yamada, Kenji Kinoshita, Gary Ngai-Wa Leung, Hideaki Ishii

{"title":"A broad-spectrum peptide screening method using an optimized solid-phase extraction and liquid chromatography-high-field asymmetric ion mobility spectrometry-mass spectrometry for doping control in equine urine.","authors":"Kohei Ohnuma, Misato Hirano-Kodaira, Michiko Bannai, Yoshibumi Shimizu, Masayuki Yamada, Kenji Kinoshita, Gary Ngai-Wa Leung, Hideaki Ishii","doi":"10.1039/d4ay01477d","DOIUrl":"10.1039/d4ay01477d","url":null,"abstract":"The abuse of prohibited peptide-based drugs with a broad spectrum of chemical characteristics poses a significant concern for the horseracing industry. Recently, there has been a notable increase in positive cases of small-peptide drugs reported in equine and canine sports. In addition to small peptides, large peptides (over 2 kDa) with structural diversity have also entered the market in increasing numbers as drugs for humans and livestock. However, the simultaneous analysis of both small- and large-peptide-based drugs is still challenging. In this study, a screening method was developed to cover 74 analytes, including peptides, their catabolites, and/or their mimetics, with molecular weights ranging from 0.3 kDa to greater than 5 kDa. The simultaneous extraction of both small and large peptides was achieved using a weak cation-exchange solid-phase extraction cartridge with a mixture of different pore sizes (suitable for large peptides), followed by analysis using liquid chromatography high-field asymmetric ion mobility spectrometry tandem mass spectrometry (LC-FAIMS-MS/MS). For method validation, the limits of detection (LoDs), reproducibility, recovery, matrix effect, selectivity, and carryover were evaluated. Remarkably, the LoDs of ∼80% of the analytes were less than or equal to 50 pg ml-1, with the lowest LoD (1 pg ml-1) being observed for selected peptides in horse urine. These results indicate a substantial advancement in achieving comprehensive coverage for both small and large peptides with high sensitivity for the purpose of doping control in horseracing and equestrian sports.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced ICP-MS detection of 24 elemental impurities in complex, high matrix mineral, medicinal lanthanum carbonates according to ICH Q2 (R2). 根据 ICH Q2 (R2)，增强 ICP-MS 对复杂、高基质矿物药用碳酸镧中 24 种元素杂质的检测。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-11-06 DOI: 10.1039/d4ay01589d

Meng-Meng Shen, Sun-Xin Zhou, Yi-Wen Jing, Chao-Qiang Xiao, Shu-Wang He, Jie Yang

{"title":"Enhanced ICP-MS detection of 24 elemental impurities in complex, high matrix mineral, medicinal lanthanum carbonates according to ICH Q2 (R2).","authors":"Meng-Meng Shen, Sun-Xin Zhou, Yi-Wen Jing, Chao-Qiang Xiao, Shu-Wang He, Jie Yang","doi":"10.1039/d4ay01589d","DOIUrl":"https://doi.org/10.1039/d4ay01589d","url":null,"abstract":"Validation of the specificity of ICP-MS methods by ICH Q2 (R2) through spiking experiments cannot characterize the multi-atomic interference of complex high matrix samples, possibly affecting the accuracy of the method and reliability of results. In this study, we highlight this issue by investigating the elemental impurities in lanthanum carbonate raw materials and use isotope ratio analysis to overcome this limitation and establishing an accurate ICP-MS method for 24 elemental impurities (listed in ICH Q3D). An Agilent 7900 ICP-MS was used with an automatic sampler for sample determination in the He mode. The isotope ratio of elemental impurities in the spiked samples was analyzed to characterize polyatomic interference and select the exclusive mass number of elemental impurities. While the recovery rate of most elemental impurities met the requirements, that of selenium (default measurement mass number 78Se) exceeded the standard in the matric sample. The isotope ratio of Se (78Se/82Se) was much higher than the theoretical value, indicating that the response intensity of m/z 78 was formed by the combination of 78Se and other mass-to-charge ratios, such as LaOH2+ formed by the large amount of La in the matrix. Therefore, 82 was chosen as the mass number for Se. The validation results indicate that the method has strong specificity, high accuracy, and is simple and facile. This study provides technical support for the control of elemental impurities in lanthanum carbonates and isotope ratios can be used as a supplementary means of spiked recovery rates.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A rapid method for assessing seed drought resistance using integrated ID-BOA-SVM. 利用集成 ID-BOA-SVM 快速评估种子抗旱性的方法。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-11-05 DOI: 10.1039/d4ay01455c

Qiaohan Wu, Xiaoyu Zhao, Biqing Zhou, Jiangcheng Liao, Qian Luo, Yue Zhao, Lijing Cai, Zhe Zhai, Liang Tong

{"title":"A rapid method for assessing seed drought resistance using integrated ID-BOA-SVM.","authors":"Qiaohan Wu, Xiaoyu Zhao, Biqing Zhou, Jiangcheng Liao, Qian Luo, Yue Zhao, Lijing Cai, Zhe Zhai, Liang Tong","doi":"10.1039/d4ay01455c","DOIUrl":"https://doi.org/10.1039/d4ay01455c","url":null,"abstract":"This study investigates the application of near-infrared spectroscopy (NIR) for assessing drought resistance in seeds, aiming to offer a rapid and efficient method suitable for large-scale primary screening. NIR spectroscopy is utilized to analyze four key factors (water, sugars, amino acids content, and genes) associated with maize seed drought responses. Signature NIR bands indicative of drought resistance-related molecules are identified using the Competitive Adaptive Reweighted Sampling (CARS) technique. Furthermore, an Improved Discrete Bayesian Optimization Support Vector Machine (ID-BOA-SVM) classification model is developed to address issues related to sparse features in traditional Bayesian Optimization Support Vector Machines (BOA-SVM). To enhance classification performance, a stacking model integrating Random Forest (RF), ID-BOA-SVM, Logistic Regression (LR), and Gradient Boosted Decision Trees (GBDT) classifiers is constructed, ensuring robustness and minimizing overfitting risks. The model achieves satisfactory recognition accuracy (94.28% accuracy, 94% precision, 94.61% recall, and 94.23% F1-score) even under conditions of substantial interference and dataset variability. This research demonstrates that NIR spectroscopy-derived data can support genetic and physiological studies of drought-resistant seed varieties, facilitating a deeper understanding of drought resistance mechanisms and optimizing breeding strategies.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-throughput optimisations for 3D chemical imaging of pharmaceutical solid oral dosage forms. 药物固体口服剂型三维化学成像的高通量优化。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-11-04 DOI: 10.1039/d4ay01806k

Liam Davison-Gates, Andrew V Ewing, Don Clark, Fiona C Clarke

{"title":"High-throughput optimisations for 3D chemical imaging of pharmaceutical solid oral dosage forms.","authors":"Liam Davison-Gates, Andrew V Ewing, Don Clark, Fiona C Clarke","doi":"10.1039/d4ay01806k","DOIUrl":"https://doi.org/10.1039/d4ay01806k","url":null,"abstract":"Chemical imaging of pharmaceutical solid oral dosage forms is a key technique for quality assurance and issue diagnosis. This technique can be further augmented using 3D chemical imaging via serial sections and image stacking. However, the additional collection time this entails means that 3D imaging is utilised for a very niche set of applications. Previous attempts have been made to optimize the process but have often compromised the quality of the resulting chemical images to achieve the gains in process time. In this study, several optimisation strategies are employed to increase the efficiency of 3D chemical image collection without sacrificing the quality of the final chemical images. The use of automated microscope macros and a kinematic mounting system allowed for rapid sample processing and efficient utilisation of equipment time. The automated macros allow the Raman microscope to collect mapping data continuously from multiple samples without the need for operator intervention steps. The kinematic mounting system allows rapid and accurate sample transfer and positioning between instruments. These optimisations resulted in a three times speed increase in collection time while keeping the same signal-to-noise ratio of the resulting chemical images. These optimisations will allow the rapid collection of statistically robust 3D chemical image data within a set time frame that is more amenable to an industrial workflow.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A sensitive SERS-based assay technique for accurate detection of foodborne pathogens without interference. 一种基于 SERS 的灵敏检测技术，可在无干扰的情况下准确检测食源性病原体。

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-11-04 DOI: 10.1039/d4ay01555j

Xiangru Bai, Wei Luo, Wenyu Zhou, Wei Chen, Xinling Guo, Aiguo Shen, Jiming Hu

{"title":"A sensitive SERS-based assay technique for accurate detection of foodborne pathogens without interference.","authors":"Xiangru Bai, Wei Luo, Wenyu Zhou, Wei Chen, Xinling Guo, Aiguo Shen, Jiming Hu","doi":"10.1039/d4ay01555j","DOIUrl":"https://doi.org/10.1039/d4ay01555j","url":null,"abstract":"The accurate and sensitive detection of foodborne pathogens is critical for timely food quality supervision and human health. To address this issue, herein, we developed a simple and novel surface-enhanced Raman scattering (SERS) assay using p-mercaptobenzoic acid (MBN)-modified gold nanoparticles (Au NPs) and magnetic beads for interference-free detection of Escherichia coli (E. coli). This assay technique cleverly reduced silver ions (Ag+) on the surface of E. coli (bacteria@Ag NPs), and the functionalized magnetic beads (capture probes) captured and enriched bacteria@Ag NPs, forming the structure of the capture probes-bacteria@Ag NPs. Then, the capture probes-bacteria@Ag NPs were dissolved in the acidic medium, and the Ag NPs on the surface of E. coli was converted to Ag+ again. Due to the special coordination between Ag+ and MBN-modified Au NPs (functionalized Au NPs), the SERS intensity of MBN exhibited a positive correlation with the E. coli concentration, and the SERS detection assay of E. coli was established. The signal of the functionalized Au NPs located at 2228 cm-1 perfectly avoided the spectral overlap with coexisting materials in the Raman fingerprint region, which ensured the accuracy of the technique. The controlled aggregation of the functionalized Au NPs ensured the reproducibility and reliability of the detection system; the emergence of MBs greatly reduced the reaction time and made sure the operation was rapid, simple and portable. The limit of detection (LOD) for E. coli was as low as 10 cfu mL-1, and the detection assay was successfully applied for the detection of E. coli in bottled water and milk. As a sensitive and accurate analytical technique for the detection of pathogens, this SERS-based method has great potential to be applied in the field of food safety.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0