Analytical Methods最新文献

{"title":"Highly sensitive chemiluminescence of water-soluble luminol and its application in forensic bloodstain detection†","authors":"Mingjiang Wu, Aijiao Liu, Tao Xu, Shuofeng Zhang, Jiayan Lan and Haiyang Wang","doi":"10.1039/D4AY02022G","DOIUrl":"10.1039/D4AY02022G","url":null,"abstract":"<p >Chemiluminescence (CL) provides outstanding analytical performance due to its background-free nature and exceptional sensitivity and selectivity. The forensic luminol chemiluminescence test is one of the most commonly used methods for detecting latent bloodstains. However, its applications are limited by the insolubility of luminol under physiological conditions. In this study, <em>m</em>-carboxy luminol with its hydrophilic design was found to overcome these limitations and showed a chemiluminescence intensity higher than that displayed by any other luminol-based CL assay. The superior CL performance of <em>m</em>-carboxy luminol encouraged us to further apply this derivative for imaging bloodstains, with the results implying excellent application prospects for <em>m</em>-carboxy-luminol-containing imaging solutions in criminal investigations.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 15","pages":" 2934-2937"},"PeriodicalIF":2.7,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding metabolic characteristics and molecular mechanisms of large to giant congenital melanocytic nevi: implications for melanoma risk and therapeutic targets†","authors":"Ge Song, Tao Dai, Yongqiang Ren, Yajie Chang, Pengfei Guo, Zhanwei Wang, Guiping Shen and Jianghua Feng","doi":"10.1039/D5AY00122F","DOIUrl":"10.1039/D5AY00122F","url":null,"abstract":"<p >Large to giant congenital melanocytic nevi (LGCMN) present clinical challenges due to their complex phenotypic heterogeneity and increased melanoma risk. Molecular-level research is essential for understanding the pathogenic mechanisms of LGCMN and identifying potential therapeutic targets. Tissue samples from 67 LGCMN lesions and 49 matched controls were analyzed using metabolomics and transcriptomics to identify metabolic characteristics and gene expression differences. A protein–protein interaction network and a multi-layer network of key metabolites–genes-pathways were established to explore the metabolic characteristics and gene associations with LGCMN. Metabolic analysis revealed a consistent dysregulation in amino acid metabolisms, including arginine, alanine, aspartate, glutamate, phenylalanine, and tyrosine, across LGCMN lesions and subtypes. Compared to controls, 18 upregulated metabolites and 7 downregulated metabolites were identified in LGCMN lesions. Metabolic profiles varied among LGCMN subtypes, with the trunks subtype exhibiting significant alterations in branched-chain amino acids. Network analysis identified 23 genes related to melanogenesis and amino acid metabolism, including TYR, SOX10, and MITF, which showed strong correlation with tyrosine, phenylalanine, and branched-chain amino acids (<em>r</em> &gt; 0.6). High centrality values for genes (<em>e.g.</em>, EDNRB, TYR, MITF, SOX10, and MAPK3 &gt; 0.300) and amino acids (<em>e.g.</em>, tyrosine at 0.397 and phenylalanine at 0.374) emphasize their pivotal roles in melanogenesis. This study reveals significant metabolic and molecular differences between LGCMN lesions, normal skin, and across LGCMN subtypes, highlighting the deregulation of amino acid metabolism and key genes involved in melanogenesis. These insights enhance our understanding of LGCMN's biological heterogeneity and provide novel avenues for therapeutic intervention.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 16","pages":" 3229-3238"},"PeriodicalIF":2.7,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ay/d5ay00122f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A binuclear zinc complex (Zn2L) as ratiometric probe for the pyrophosphate (PPi) sensing†","authors":"Mengxia Liu, Wenwen Sun, Le Wang, Zhiqiang Niu, Xiao Zhang, Dingjia Xiao, Tianjia Xiao and Junfeng Wang","doi":"10.1039/D5AY00028A","DOIUrl":"10.1039/D5AY00028A","url":null,"abstract":"<p >Phosphate anion (PPi) as hydrolysis products of adenosine triphosphate (ATP), is related to various biochemical reactions, particularly energy storage and transfer, nucleotide metabolism, and cellular signaling. In this paper, a binuclear zinc complex (Zn<small>₂</small>L) was prepared for the PPi sensing in Tris–HCl solution. The results showed a clear fluorescence change from blue to green (425 nm → 500 nm) with the addition of PPi to the probe. The probe Zn<small>₂</small>L demonstrated a remarkable selectivity for PPi in comparison to common anions, with an estimated limit of detection (LOD) of 0.29 μM within a working range of 0–9 μM. UV titration experiments showed two binding steps from 1 : 1 to 1 : 2 complex (Zn<small>₂</small>L–PPi). Density-functional theory (DFT) studies were further conducted to find an energy gap that decreases with complex (probe + 2PPi) formation, resulting in a redshift of the absorption spectrum (360 nm → 430 nm). In addition, the Zn<small>₂</small>L probe was also used for PPi sensing in a urine sample, in which relative standard deviation values (1.19–2.17%) and recoveries (95.00–103.00%) were obtained.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 14","pages":" 2859-2866"},"PeriodicalIF":2.7,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using the orchard-to-fork approach in designing nutritional trials: the apple phenolics case†","authors":"Linda Nezbedova, Tony McGhie, Sunali Mehta, Mark Christensen, Noha Ahmed Nasef and Julian Heyes","doi":"10.1039/D4AY02017K","DOIUrl":"10.1039/D4AY02017K","url":null,"abstract":"<p >Nutritional trials often yield inconsistent results due to the lack of standardised study designs, especially when evaluating the health benefits of fruits and vegetables. Apple phenolics, linked to various health benefits, have particular challenges in human nutritional studies due to compositional changes influenced by factors like fruit maturity, growing conditions, tissue type, and processing methods. This study applied an ‘orchard-to-fork’ approach to evaluate Monty's Surprise apple phenolics in the skin and flesh, controlling for harvest procedures, fruit maturity, and seasonal impact. It further assessed processing methods to preserve phenolic content, with the goal of developing a standardised material for dietary intervention trials. Monty's Surprise apples were collected over two seasons following standardised harvesting protocols and various fruit maturity parameters (firmness, starch pattern, and total soluble solids) were controlled and measured for various apple sizes. Phenolic content in the skin and flesh was analysed with LC-HRAM-MS and compared across seasons. The impacts of processing (pureeing, air drying, and slicing) on phenolic content were also evaluated. Cold storage for four weeks reduced maturity variability across apple sizes. Monty's Surprise apple skin (5270.49 ± 214.95 mg kg<small>⁻¹</small> FW) had significantly (<em>p</em> &lt; 0.0001) higher phenolic concentrations than the flesh (863.69 ± 12.71 mg kg<small>⁻¹</small> FW), with procyanidins as the main phenolic group. No seasonal impact was observed. Pureed apples retained significantly higher phenolics compared to sliced or dehydrated samples. Monty's Surprise apple is a rich source of health-beneficial phenolics, and pureeing effectively preserves these compounds. This study offers guidance for the development of standardised apple material for dietary intervention trials to more accurately investigate the health benefits of apples.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 14","pages":" 2878-2886"},"PeriodicalIF":2.7,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a method for the detection of fungicide residues in foods of animal origin based on modified QuEChERS-GC-MS/MS†","authors":"Yukun Huang, Qianran Sun, Ping Li, Jun Liu, Yuan Gou, Wei Dong and Xuemei Wu","doi":"10.1039/D4AY02291B","DOIUrl":"10.1039/D4AY02291B","url":null,"abstract":"<p >In this study, an approach involving gas chromatography-mass spectrometry (GC-MSMS) combined with a modified QuEChERS method was developed for the detection of 13 novel fungicides in chicken and beef samples, in accordance with the regulatory limits proposed by Japan, South Korea, Codex Alimentarius, and the EU in recent years. The regulatory limits and detection methods for these fungicides in foods of animal origin are yet to be established in China. These 13 fungicides were linearly correlated in a range of 0.1–200 ng mL<small>⁻¹</small> with a coefficient of determination (<em>R</em><small>²</small>) of &gt;0.999. The limits of detection (LODs) and limits of quantification (LOQs) for the 13 fungicides were found to be in a range of 0.16–1.50 ng mL<small>⁻¹</small> and 0.54–5.00 ng mL<small>⁻¹</small>, respectively. The recovery of the spiked samples exhibited a range of 79.51–111.39% (RSD ≤ 10.51%), demonstrating satisfactory accuracy and precision. This study establishes a foundation for the identification of novel fungicides in foods of animal origin and provides technical references for the investigation of novel fungicides.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 14","pages":" 2850-2858"},"PeriodicalIF":2.7,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitive detection of H2O2 based on a MWCNTs/Ti3C2 nanocomposite-modified electrode","authors":"Wei Liu, Hongfang Li, Deming Li, Xiaoting Liu, Lei Liu, Zhiyong Zhu, Haoyu Hao, Qiongyao Zeng and Yanhua Sun","doi":"10.1039/D5AY00120J","DOIUrl":"10.1039/D5AY00120J","url":null,"abstract":"<p >In this work, MWCNTs/Ti<small>₃</small>C<small>₂</small> nanocomposites were synthesized by a convenient method and characterized in terms of structure and properties by SEM, FT-IR, XPS and electrochemical methods. The electrochemical sensor developed with MWCNTs/Ti<small>₃</small>C<small>₂</small> nanocomposites exhibits good electrochemical properties. The sensor demonstrates excellent electrocatalytic performance for H<small>₂</small>O<small>₂</small> and was used to detect the concentration of H<small>₂</small>O<small>₂</small>. The linear range of the sensor was 0.05–1080 μM, with a detection limit of 0.017 μM (S/N = 3). The sensor shows strong anti-interference performance, stability and reproducibility and exhibits good performance in real sample detection.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 16","pages":" 3210-3219"},"PeriodicalIF":2.7,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A turn-off fluorescent chemical sensor based on a thiazole-Schiff base structure for highly selective and accurate detection of Cu2+ in living cells†","authors":"Qian Sun, Lu Ren, Jing Liu, Zhaoyun Yang, Dawei Zhang and Shuangbao Li","doi":"10.1039/D5AY00243E","DOIUrl":"10.1039/D5AY00243E","url":null,"abstract":"<p >A Schiff base fluorescent probe, HTT, based on a sulfonylhydrazone structure was designed and synthesized for the sensitive and selective detection of Cu<small>²⁺</small>. The probe HTT exhibits good anti-interference performance toward Cu<small>²⁺</small> in the presence of a variety of metal ions. After the addition of Cu<small>²⁺</small>, it can quickly respond within 40 seconds, and the fluorescence detection limit is 1.10 nM. The coordination ratio of probe HTT and Cu<small>²⁺</small> is 2 : 1, and the coordination reaction between C<img>N, S<img>O and Cu<small>²⁺</small> limits the formation of hydrogen bonds between the hydroxyl group and C<img>N, disrupting the spatial coplanar effect of the probe molecule and thereby inducing fluorescence quenching. The probes can be recovered and reused using EDTA for the detection of Cu<small>²⁺</small>. The probe was also applied to the successful monitoring of Cu<small>²⁺</small> in living cells and real water samples.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 15","pages":" 3036-3044"},"PeriodicalIF":2.7,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel poly-deep eutectic solvent-functionalized magnetic graphene oxide nanomaterials for high-performance solid-phase extraction of trypsin†","authors":"Jing Chen, Xiyan He and Yuzhi Wang","doi":"10.1039/D5AY00252D","DOIUrl":"10.1039/D5AY00252D","url":null,"abstract":"<p >Three novel polymethacrylic acid-based deep eutectic solvent (DES)-functionalized magnetic graphene oxide composites were designed and successfully synthesized <em>via</em> the radical polymerization method. The aim was to achieve the selective solid-phase extraction of trypsin (Tryp). Among them, the magnetic extractant (MGO@PDES<small>₂</small>) functionalized with a poly-deep eutectic solvent composed of tetraethylammonium chloride and methylpropionic acid at a molar ratio of 1 : 2 exhibited the highest extraction efficiency for Tryp. Owing to the high specific surface area of MGO and the abundant carboxyl functional groups in PDES<small>₂</small>, the prepared MGO@PDES<small>₂</small> exhibited excellent selectivity and stability for Tryp extraction. Under optimal conditions, the extraction capacity of Tryp by MGO@PDES<small>₂</small> reached 708.85 mg g<small>⁻¹</small>. The extraction driving forces between MGO@PDES<small>₂</small> and Tryp were hydrogen-bonding interactions and electrostatic interactions. Among the seven biomacromolecules, MGO@PDES<small>₂</small> displayed outstanding selectivity for Tryp. In addition, the results of cycling experiments indicated that MGO@PDES<small>₂</small> could be reused many times without a significant change in extraction capacity. Moreover, the proposed method was successfully applied to extract Tryp from the crude extract of bovine pancreas, yielding satisfactory results. All the results suggest that MGO@PDES<small>₂</small> is a promising magnetic extractant, which is expected to provide new ideas for the extraction and separation of proteins.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 16","pages":" 3132-3144"},"PeriodicalIF":2.7,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baseline matching preprocessing of temperature perturbation infrared spectra","authors":"Robert L. White","doi":"10.1039/D5AY00196J","DOIUrl":"10.1039/D5AY00196J","url":null,"abstract":"<p >An infrared spectrum baseline matching procedure that compensates for measurement drift and eliminates sloping baselines from sequentially acquired spectra is described. The theory underlying this procedure is provided and examples are given for three implementations based on infrared spectrum data sets containing at least 100 successively measured spectra. Consecutive spectra were acquired when the infrared beam contained: no sample, poly(styrene) powder, and a poly(styrene) film. The first two data sets consisted of 120 spectra and were used to characterize instrument reproducibility and identify short- and long-term measurement drifts. The 200 infrared spectra obtained while heating and then cooling a poly(styrene) film were subjected to baseline matching to reveal subtle temperature-dependent changes that are not evident when overlayed spectra are displayed. Baseline matching preprocessing is easily implemented on large numbers of similar spectra by using macro programming.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 14","pages":" 2917-2924"},"PeriodicalIF":2.7,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ay/d5ay00196j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An electrochemical DNA biosensor based on denatured vesicle-mediated chain exchange amplification combined with electric field-assistance for nucleic acid detection†","authors":"Jinling Cui, Ritong Sun, Yu Liao, Yanling Wang, Chao Jiang, Lei Wang, Chao Shi and Cuiping Ma","doi":"10.1039/D5AY00094G","DOIUrl":"10.1039/D5AY00094G","url":null,"abstract":"<p >Electrochemical DNA biosensors have been extensively used in food safety, clinical medicine and environmental monitoring due to their high specificity and sensitivity. However, electrochemical DNA biosensors based on nucleic acid hybridization still face challenges in achieving rapid and sensitive detection. In this study, a sensitive and rapid electrochemical DNA biosensor was developed using Strand Exchange Amplification (SEA) technology, with its performance evaluated against the bovine genome as the target. Additionally, gold nanoparticles (AuNPs) were employed to modify the electrode surface, a strategy to enhance both the density of probe modification and the amplification efficiency. Furthermore, the biosensor's sensitivity has been shown to be augmented by the exceptional conductivity of AuNPs. Despite the biosensor's simplicity and sensitivity, the detection time remains a limiting factor. To address this, the incorporation of an electric field within the biosensor framework has been proposed as a strategy to enhance the coupling rate of the nucleic acid amplification and streptavidin–biotin systems. This modification is anticipated to reduce the overall detection time, enabling rapid and precise real-time nucleic acid analysis. The biosensor demonstrated the capability to detect genome DNA as low as 1 fg μL<small>⁻¹</small> within 65 min, underscoring its significant potential for applications, such as detecting meat adulteration.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 13","pages":" 2657-2664"},"PeriodicalIF":2.7,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}