Analytical Methods最新文献_第4页

A xylenol orange-based pH-sensitive sensor array for identification of bacteria and differentiation of probiotic drinks.

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-12-10 DOI: 10.1039/d4ay01982b

Changmao Yang, Yue Xiao, Yunjun Yan, Houjin Zhang

{"title":"A xylenol orange-based pH-sensitive sensor array for identification of bacteria and differentiation of probiotic drinks.","authors":"Changmao Yang, Yue Xiao, Yunjun Yan, Houjin Zhang","doi":"10.1039/d4ay01982b","DOIUrl":"https://doi.org/10.1039/d4ay01982b","url":null,"abstract":"The development of straightforward and cost-efficient methods for bacterial identification is very important. In this study, we utilized xylenol orange, metal ions, and diverse bacterial carbon sources to construct a sensor array, achieving precise bacterial identification. Initially, we examined the absorbance variations of xylenol orange with five metal ions (Mn2+, Co2+, Ni2+, Cu2+, and Zn2+) at pH levels ranging from 4 to 7, observing significant changes at 570 nm or 580 nm. Given that bacteria can generate varying amounts of acid in different carbon sources, we employed a blend of xylenol orange and the five metal ions as pH-sensitive probes to characterize bacterial metabolism in three carbon sources, resulting in the development of a five-sensor array that effectively differentiated seven bacteria. Additionally, by utilizing xylenol orange with Co2+, we successfully identified six different bacterial mixtures and five types of probiotic drink products. These findings highlight the potential of our methods for broader practical application in bacterial identification in practical use.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized separation of astaxanthin stereoisomers from microbial sources using chiral HPLC.

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-12-10 DOI: 10.1039/d4ay01768d

Xuehua Han, Xin Wang, Yanhong Chen, Yuanfan Yang, Xiping Du, Zhipeng Li, Zedong Jiang, Hui Ni, Qingbiao Li

{"title":"Optimized separation of astaxanthin stereoisomers from microbial sources using chiral HPLC.","authors":"Xuehua Han, Xin Wang, Yanhong Chen, Yuanfan Yang, Xiping Du, Zhipeng Li, Zedong Jiang, Hui Ni, Qingbiao Li","doi":"10.1039/d4ay01768d","DOIUrl":"https://doi.org/10.1039/d4ay01768d","url":null,"abstract":"Astaxanthin (AST) is a high-value antioxidant, and its efficient isolation and utilization are challenging owing to the presence of different stereoisomers from various sources. In the present study, a semi-preparative HPLC method for the efficient separation of AST stereoisomers using a Chiralpak IC chiral column with good loading capacity and chiral recognition ability was successfully developed. The mobile phase was methanol-methyl tert-butyl ether (90 : 10, v/v), with a flow rate of 3.06 mL min-1 and a maximum injection volume of 0.32 mg. The results indicated that the purity of all-trans AST was 97.9% for Haematococcus pluvialis and 97.5% for Phaffia rhodozyma. Additionally, molecular weights and fragmentation patterns analyzed using mass spectrometry were consistent with those of all-trans AST. Linearity validation and reproducibility experiments revealed that all calibration curves had coefficients of determination (R2) greater than 0.999 and a relative standard deviation (RSD) of <3.8%. This is because all-trans AST stereoisomers could undergo specific rotations or spins due to π-π interactions, hydrogen bonding, and inclusion interactions. This process allowed the successful separation of the three all-trans AST optical isomers and provides a theoretical basis for large-scale preparation of all-trans AST stereoisomers from different sources.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An AIE active luminogen derived using 2-hydroxy-1-naphthaldehyde and 3-hydroxy-2-naphthohydrazide for the detection of sparfloxacin and azithromycin.

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-12-09 DOI: 10.1039/d4ay01706d

Dhvani A Patel, Bigyan R Jali, Suban K Sahoo

{"title":"An AIE active luminogen derived using 2-hydroxy-1-naphthaldehyde and 3-hydroxy-2-naphthohydrazide for the detection of sparfloxacin and azithromycin.","authors":"Dhvani A Patel, Bigyan R Jali, Suban K Sahoo","doi":"10.1039/d4ay01706d","DOIUrl":"https://doi.org/10.1039/d4ay01706d","url":null,"abstract":"Herein, an aggregation-induced emission (AIE) luminogen (AIEgen) NANH was developed by condensing equimolar amounts of 2-hydroxy-1-naphthaldehyde and 3-hydroxy-2-naphthohydrazide. The AIE behaviour of NANH was explored in a mixed DMSO-H2O (HEPES buffer, pH 7.4, 10 mM) medium. The self-aggregates of NANH show enhanced emission at HEPES fractions (fHEPES) ≥70%. AIEgen NANH (fHEPES 95%, λex = 390 nm, λem = 490 nm) was applied for the fluorescence turn-off sensing of sparfloxacin and azithromycin. With the addition of different analytes, the fluorescence intensity of AIEgen NANH was quenched by sparfloxacin and azithromycin. No noticeable changes in the fluorescence of AIEgen NANH were observed with other added analytes. Experimental evidence supported that the fluorescence quenching of AIEgen NANH by sparfloxacin and azithromycin is static in nature. From the fluorescence titrations, the detection limits of AIEgen NANH were estimated down to 0.64 μM and 0.65 μM for sparfloxacin and azithromycin, respectively. Further, blood serum samples spiked with sparfloxacin and azithromycin were used to examine the analytical utility of AIEgen NANH.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast and effective assessment of 4-chlorophenol as a persistent organic pollutant (POP) using a multi-walled carbon nanotube-modified screen-printed carbon electrode (C/MWCNT-COOH/SPCE).

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-12-09 DOI: 10.1039/d4ay01916d

Ayman H Kamel, Ayman Alnakkal, Hisham S M Abd-Rabboh, A Hefnawy

{"title":"Fast and effective assessment of 4-chlorophenol as a persistent organic pollutant (POP) using a multi-walled carbon nanotube-modified screen-printed carbon electrode (C/MWCNT-COOH/SPCE).","authors":"Ayman H Kamel, Ayman Alnakkal, Hisham S M Abd-Rabboh, A Hefnawy","doi":"10.1039/d4ay01916d","DOIUrl":"https://doi.org/10.1039/d4ay01916d","url":null,"abstract":"In this study, a rapid, precise, and targeted electroanalytical method was developed for the trace determination of 4-chlorophenol (4-CP). The study reports the use of cyclic voltammetry (CV) to characterize the electrochemical response of 4-CP and the optimization of differential pulse voltammetry (DPV) settings for its sensitive quantification. Screen-printed carbon electrodes (SPCEs) were selected for the sensitive detection of 4-CP using DPV. The incorporation of multi-walled carbon nanotubes functionalized with carboxyl groups (MWCNT-COOH) as a modifier on the working SPCE significantly enhances the electrode's performance, resulting in a 5-fold increase in sensitivity compared to that of the unmodified SPCE. Under optimal conditions, oxidation peak current exhibited a detection limit of 9.2 nM and was proportional to 4-CP concentration in the range of 0.01-1.3 μM. Additionally, the constructed sensor demonstrated high stability, high selectivity, good reproducibility, and excellent feasibility. These findings suggest that the C/MWCNT-COOH/SPE offers a simple, rapid, and cost-effective method for the prospective online assessment of 4-CP in various samples with different matrices.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A label-free colorimetric aptasensor for rapid multiplex detection of leuco-malachite green and leuco-crystal violet.

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-12-09 DOI: 10.1039/d4ay01849d

Runze Zhang, Kexin Liu, Fangran Jia, Tianci Xu, Yongjie Yang

{"title":"A label-free colorimetric aptasensor for rapid multiplex detection of leuco-malachite green and leuco-crystal violet.","authors":"Runze Zhang, Kexin Liu, Fangran Jia, Tianci Xu, Yongjie Yang","doi":"10.1039/d4ay01849d","DOIUrl":"https://doi.org/10.1039/d4ay01849d","url":null,"abstract":"Leuco-malachite green (LMG) and leuco-crystal violet (LCV) are widespread co-pollutants in aquatic products that pose a severe threat to human health. Therefore, it is urgent and challenging to develop rapid multiplex detection of LMG and LCV. Herein, the bispecific aptamer (A5b) for LMG and LCV was characterized. Its dissociation constant (Kd) was 2.03 ± 0.13 μM for LMG and 0.97 ± 0.06 μM for LCV. Then, we used A5b as a sensing probe to develop a label-free colorimetric aptasensor using gold nanoparticles (AuNPs) as an indicator and poly-diallyl dimethylammonium chloride (PDDA) as an aggregation inducer. In the absence of LMG and LCV, PDDA hybridized A5b via electrostatic interaction to form a \"duplex\" structure, which failed to induce the aggregation of AuNPs. However, in the presence of LMG or LCV, A5b specifically bound to LMG or LCV, releasing PDDA. The free PDDA then induced the aggregation of AuNPs. Consequently, a visible color change from red to blue was observed with increasing LMG/LCV concentration. This sensor exhibited excellent sensitivity and bispecificity, and a limit of detection (LOD) of 77.9 nM for LCV and 123.1 nM for LMG, with a linear concentration range of 1-6 μM for LCV and 1-8 μM for LMG. Additionally, the potential of this sensor for real-world applications was well demonstrated in crucian samples. Overall, this work provided a bispecific affinity probe for LMG and LCV for the first time and reported a label-free colorimetric aptasensor for multiplex detection of LMG and LCV in aquatic products.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Separation and preparation of nanoparticles of urban dust for biological studies.

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-12-09 DOI: 10.1039/d4ay01592d

Alexandr I Ivaneev, Mikhail S Ermolin, Roman A Zinovkin, Anna A Dashkevich, Ludmila A Zinovkina, Boris V Chernyak, Petr S Fedotov

{"title":"Separation and preparation of nanoparticles of urban dust for biological studies.","authors":"Alexandr I Ivaneev, Mikhail S Ermolin, Roman A Zinovkin, Anna A Dashkevich, Ludmila A Zinovkina, Boris V Chernyak, Petr S Fedotov","doi":"10.1039/d4ay01592d","DOIUrl":"10.1039/d4ay01592d","url":null,"abstract":"Nanoparticles (NPs) of urban dust pose a potential threat to public health. Nevertheless, this issue remains largely unexplored due to a lack of biological research related to these NPs. This may be attributed to the complexity of the separation, characterization, analysis, and subsequent preparation of NPs of urban dust for biological studies. In the present work, the methodology for the separation and preparation of NPs of urban dust for biological assays has been developed. The isolation of NPs from bulk samples of urban dust has been carried out using coiled tube field-flow fractionation, which allows one to recover a fraction of NPs, which is sufficient for further research. The weight of the recovered fraction of NPs was 0.42 ± 15 mg; the mean size of particles in the fraction was 220 nm. Albumin was employed as a stabilizing agent for NPs, a phosphate buffer solution simulated physiological salt concentrations. The isolated NPs were found to contain microorganisms, and a sterilization procedure was therefore applied. A 5 min UV treatment ensured sterilization of the suspension of NPs. Ultrafiltration was used to pre-concentrate NPs of urban dust required for biological studies; the ultrafiltration procedure did not affect the stability of the NPs suspension. The concentrations of toxic elements like Cu, Zn, As, Mo, Cd, Sn, Sb, Hg, Pb and Bi in the obtained NPs were found to be up to 10 times higher than in the bulk dust samples, indicating a potential health threat. The proposed procedure for the separation and preparation of NPs of urban dust can serve as a reliable basis for further biological studies.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142793960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant activity of herbal medicine Pyrrosia lingua evaluated by electron paramagnetic resonance spectroscopy.

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-12-09 DOI: 10.1039/d4ay02045f

Li Quan, En-Chao Zhou, Xue-Wen Guo, Gui Yin

{"title":"Antioxidant activity of herbal medicine Pyrrosia lingua evaluated by electron paramagnetic resonance spectroscopy.","authors":"Li Quan, En-Chao Zhou, Xue-Wen Guo, Gui Yin","doi":"10.1039/d4ay02045f","DOIUrl":"https://doi.org/10.1039/d4ay02045f","url":null,"abstract":"The hydroxyl radical (˙OH) is one of the most reactive and harmful reactive oxygen species in living organisms. In this study, we used electron paramagnetic resonance (EPR) spectroscopy to evaluate the antioxidant activity of herbal medicine Pyrrosia lingua (PL) by measuring its scavenging activity against ˙OH radicals. Using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical as a scavenging model, the PL extract (0.6 mg mL-1) was able to scavenge 95.1% of 0.5 mmol L-1 DPPH radicals, showing a quantitative linear relationship between the DPPH radical concentration and the double integral area under the characteristic peaks of the EPR spectra, with a coefficient of determination R2 = 0.9986. Additionally, we also studied the scavenging activity of the PL extract towards hyperactive ˙OH radicals through the reduction of the intensity of the DMPO-OH˙ adducts. The PL extract (0.5 mg mL-1) showed a high scavenging ability against extremely short-lived ˙OH radicals, with a scavenging rate of 93.6% for ˙OH radicals generated by continuous ultraviolet-irradiation of a 5 mM H2O2 aqueous solution for 30 minutes. This study developed a method based on EPR spectroscopy measurements to detect ˙OH radicals captured by herbal medicine, providing a reference for the application of PL in antioxidant and clinical disease therapies.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An electrochemical aptasensor based on C-ZIF-67@PAN nanofibers for detection of ampicillin in milk.

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-12-09 DOI: 10.1039/d4ay02001d

Yuting Luo, Yiwei Sun, Haoxiang Wang, Yuyang He, Yuxun Zhang, Hailu Lei, Hong Yang, Jinhao Wei, Dongpo Xu

引用次数: 0

Electroanalytical overview: the use of laser-induced graphene sensors.

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-12-09 DOI: 10.1039/d4ay01793e

Robert D Crapnell, Elena Bernalte, Rodrigo A A Muñoz, Craig E Banks

{"title":"Electroanalytical overview: the use of laser-induced graphene sensors.","authors":"Robert D Crapnell, Elena Bernalte, Rodrigo A A Muñoz, Craig E Banks","doi":"10.1039/d4ay01793e","DOIUrl":"10.1039/d4ay01793e","url":null,"abstract":"Laser-induced graphene, which was first reported in 2014, involves the creation of graphene by using a laser to modify a polyimide surface. Since then, laser-induced graphene has been extensively studied for application in different scientific fields. One beneficial approach is the use of laser-induced graphene coupled with electrochemistry, where there is a growing need for disposable, conductive, reproducible, flexible, biocompatible, sustainable, and economical electrodes. In this mini overview, we explore the use of laser-induced graphene as the basis of electroanalytical sensors. We first introduce laser-induced graphene, before moving to the use of laser-induced graphene electrodes highlighting the various approaches and different laser parameters used to produce different graphene micro and macro structures, whilst describing how these structures are characterised and benchmarked for those working in the field of laser-induced graphene electrodes for comparison aspects. Next, we turn to the use of laser-induced graphene electrodes as the basis of electrochemical sensing platforms towards key analytes and its use in the development of biosensors. We provide a critical overview of the use of laser-induced graphene sensors compared to screen-printed and additive manufactured electrodes, providing future suggestions for the field.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142793950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid detection of volatile organic compounds emitted from plants by multicapillary column-ion mobility spectrometry.

IF 2.7 3区化学

Analytical Methods Pub Date : 2024-12-09 DOI: 10.1039/d4ay01817f

Vahideh Ilbeigi, Younes Valadbeigi, Martina Zvaríková, Peter Fedor, Štefan Matejčík

引用次数: 0