Analytical Methods最新文献

{"title":"Determination of paclobutrazol using square wave voltammetry based on a molecularly imprinted polymer and boron-doped copper oxide/graphene nanocomposite.","authors":"Çağdaş Salih Meriç, Haci Ahmet Deveci, Müge Mavioğlu Kaya, Ayla Deveci, Neslihan Özdemir, Havva Boyacıoğlu, Mehmet Lütfi Yola","doi":"10.1039/d4ay01947d","DOIUrl":"10.1039/d4ay01947d","url":null,"abstract":"<p><p>In the present study, a novel voltammetric sensor based on a boron-doped copper oxide/graphene (B-CuO-Gr) nanocomposite and molecularly imprinted polymer (MIP) was developed for the detection of paclobutrazol (PAC) in apple and orange juice samples. The B-CuO-Gr nanocomposite was prepared using sol-gel and calcination methods. After modifying glassy carbon electrodes with the B-CuO-Gr nanocomposite, PAC-imprinted electrodes were prepared in the presence of 100.0 mM pyrrole (Py) monomer and 25.0 mM PAC using cyclic voltammetry (CV). After elucidating the surface properties of the prepared B-CuO-Gr nanocomposite and PAC-imprinted electrodes using various characterization techniques, the PAC-imprinted sensor was successfully applied to apple and orange juice samples, demonstrating high recovery. A linear range of 1.0 × 10^-9 to 1.0 × 10^-8 M PAC (<i>R</i>² = 0.9983) and a detection limit (LOD) of 3.30 × 10^-10 M were observed, along with high selectivity, stability, and reproducibility for the MIP/B-CuO-Gr/GCE sensor.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"1080-1089"},"PeriodicalIF":2.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Energized dispersive guided extraction (EDGE): a promising tool for analytical chemistry applications.","authors":"Paulo N A Santos, Yasmine B Andrade, Honnara S Granja, Lisiane S Freitas, Allan S Polidoro, Elina B Caramão","doi":"10.1039/d4ay01623h","DOIUrl":"10.1039/d4ay01623h","url":null,"abstract":"<p><p>Analytical chemistry demands precise sample preparation methods to ensure accurate qualitative and quantitative determinations, especially those capable of clean-up and preconcentration of target analytes. Extraction plays a crucial role in enhancing the selectivity and sensitivity of analytical procedures. Thus, Energized Dispersive Guided Extraction (EDGE) has emerged as an innovative alternative to traditional methods, such as Soxhlet, maceration, and percolation, as well as modern techniques like Accelerated Solvent Extraction (ASE), Supercritical Fluid Extraction (SFE), and Microwave or Ultrasound Assisted Extraction (MAE and UAE). By combining dispersive solid-phase extraction (DSPE) with pressurized liquid extraction (PLE), this technique offers distinct advantages, including faster extraction times, reduced solvent consumption, and improved reproducibility. Its automated system also minimizes operator intervention, ensuring greater precision and optimizing workflows. However, there is a significant gap in studies exploring its various potential applications and perspectives. In this scenario, this is the first paper to broaden the range of applications of the EDGE system by exploring its use in food, pharmaceutical, and environmental areas. These sectors benefit from EDGE's ability to preserve analyte integrity, increase extraction yields, and promote environmentally sustainable practices. Moreover, EDGE's flexibility and efficiency make it a powerful tool for addressing emerging challenges, such as detecting contaminants in complex matrices and complying with stricter regulatory standards. Expanding EDGE's applications in these areas underscores its potential to transform analytical processes, driving advancements in food safety, pharmaceutical quality control, and environmental monitoring.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"871-881"},"PeriodicalIF":2.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142996356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-precise ruler for ammonia nitrogen quantification in electrochemical synthesis experiments.","authors":"Yao Hu, Donghui Wang, Bo Hu, Haihui Lan, Wen Zhong, Qi Wang, Huicong Xia, Mingde Yao, Mingqing Chen, Mingliang Du","doi":"10.1039/d4ay02288b","DOIUrl":"https://doi.org/10.1039/d4ay02288b","url":null,"abstract":"<p><p>The field of electrochemical ammonia synthesis has made rapid advancements, attracting a large number of scientists to contribute to this area of research. Accurate detection of ammonia is crucial in this process for evaluating the efficiency and selectivity of electrocatalysts. In this study, we systematically investigate the indophenol blue method for ammonia detection, examining the effects of key factors such as solution pH, nitrate concentration, and metal ion concentration on measurement accuracy. Based on experimental optimization and mathematical algorithms, we propose an iterative refinement method supported by custom-developed code. This method automates the generation and adjustment of calibration curves, reduces measurement errors, and enhances detection precision, offering a valuable framework for the quantitative detection of ammonia and other small molecules in electrochemical synthesis.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining digital imaging and quantum dots for analytical purposes.","authors":"João Paulo B de Almeida, Thomas Fernando Ferreira Tributino Dos Santos, José Roberto Sabino Júnior, Elias Vinicius Ferreira do Amaral, Claudinéia R S Oliveira, Matheus V Maia, Willian T Suarez, Lucas B Ayres, Carlos D Garcia, Vagner B Dos Santos","doi":"10.1039/d4ay02097a","DOIUrl":"10.1039/d4ay02097a","url":null,"abstract":"<p><p>This review provides a critical assessment of the most recent advances in digital imaging (DI) methods, applied for the development of analytical methodologies combining quantum dots (QDs). The state-of-the-art, treatment of data, instrumental considerations, software, sensing approaches, and optimization of the resulting methods are reported. Applications of the technology for the analysis of food and beverages, biomedically relevant analytes, drugs, environmental samples and forensic samples are also discussed. These examples aim to highlight the advantages of DI over traditional instrumentation, that in combination with QDs represents a powerful option for low-cost and on-site analyses. Moreover, some of these DI methods have been explored in the context of green chemistry principles, demonstrating a sustainable approach to modern analytical challenges.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"916-938"},"PeriodicalIF":2.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic characteristics of saponins from <i>Panax notoginseng</i> leaves biotransformed by gut microbiota in rats.","authors":"Jing Liu, Li Shao, Jie Zhou, Shuang-Feng Li, Jia-Meng Huang, Jing-Bo Peng, Wei Zhang, Jian-Bo Wan, Wei-Hua Huang","doi":"10.1039/d4ay01941e","DOIUrl":"10.1039/d4ay01941e","url":null,"abstract":"<p><p>Saponins are responsible for the clinical effects of <i>Panax notoginseng</i> leaves, which are traditionally produced as the single herb resource of 'Qiye Shenan Pian' in Chinese patent medicine. In this study, the metabolic characteristics of PNLSs were explored in rat feces. PNLSs as well as their metabolites were analyzed by ultra-performance liquid chromatography tandem/quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS). Subsequently, seventy-five metabolites were tentatively identified in the control group mainly due to the deglycosylation and dehydration biopathways, but only twenty low yields were determined in the pseudo-germ-free (GF) group. Ginsenoside compound K was the predominant metabolite in the control group. The data presented that gut microbiota played a pivotal role in the metabolic kinetics of PNLSs.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"972-989"},"PeriodicalIF":2.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tungsten disulphide nanosheet modulated fluorescent gold nanocluster immunoprobe for the detection of tau peptide: Alzheimer's disease biomarker.","authors":"Merin K Abraham, Anju S Madanan, Susan Varghese, Ali Ibrahim Shkhair, Geneva Indongo, Greeshma Rajeevan, Arathy B Kala, Sony George","doi":"10.1039/d4ay02014f","DOIUrl":"10.1039/d4ay02014f","url":null,"abstract":"<p><p>The neuronal tau peptide serves as a key biomarker for neurodegenerative diseases, specifically, Alzheimer's disease, a condition that currently has no cure or definitive diagnosis. The methodology to noninvasively detect tau levels from body fluids remains a major hurdle for a rapid and simple diagnostic approach. Thus, developing new detection methods for sensing tau protein levels is crucial. In this work, we report an immunoprobe based on anti-tau antibody (mAb-tau)-conjugated fluorescent gold nanoclusters (AuNCs) quenched with tungsten disulphide nanosheets (WS₂ NS) for the detection of tau protein in human serum samples. The mAb-tau conjugated probe is designed to provide a specific binding site for the tau peptide by strong antigen-antibody interface. The WS₂ NS surface quenches the fluorescence of mAb-tau@AuNCs, which is subsequently recovered by the addition of tau peptide in a linear concentration range (63.3-615.38 pg mL^-1). The enhancement in fluorescence of WS₂ NS@mAb-tau@AuNCs enables the quantification of tau peptide in concentrations pertinent to human serum tau levels in Alzheimer's patients. The developed probe achieves a limit of detection (LOD) and limit of quantification (LOQ) of 6.54 pg mL^-1 and 21.8 pg mL^-1 for tau peptide in PBS buffer. The study is further extended in spiked human serum samples, with satisfactory recovery percentages in the range of 94.37-117.53%. The technique holds promise as an immunoprobe for tau peptide detection and has potential in developing an economically viable probe for the clinical diagnosis of tau-related neurodegenerative disorders.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"1125-1136"},"PeriodicalIF":2.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of flavor components between normal and gas-producing wasabi based on HS-GC-IMS, HS-GC-MS and electronic sensory technology.","authors":"Xiao Liu, Ling-Xiao Liu, Qin-Guo Xu, Xiao-Jie Hu, Yun-Guo Liu","doi":"10.1039/d4ay01993h","DOIUrl":"https://doi.org/10.1039/d4ay01993h","url":null,"abstract":"<p><p>Wasabi is a type of sauce made from the plant horseradish. During its production and storage, gas production sometimes occurs, which leads to changes in the flavor quality of wasabi. In this study, an electronic nose, electronic tongue, headspace-gas chromatography-mass spectrometry and headspace-gas chromatography-ion mobility spectrometry combined with multivariate statistical analysis were used to compare the differences in odor, taste and volatile components between normal and gas-producing wasabi. The results showed that normal and gas-producing wasabi samples could be distinguished by the electronic nose and electronic tongue. Furthermore, 72 and 65 volatile components were identified from wasabi by headspace-gas chromatography-mass spectrometry and headspace-gas chromatography-ion mobility spectrometry analysis, respectively. In addition, 33 key volatile components that caused the difference between normal and gas-producing wasabi were identified through variable projection importance index analysis. Therefore, normal and gas-producing wasabi could be effectively distinguished and their differences in odor, taste and volatile components could be clarified by the four flavor analysis techniques combined with multivariate statistical analysis, which provide a scientific basis for the quality control and process optimization of wasabi.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dioxins and their impact: a review of toxicity, persistence, and novel remediation strategies.","authors":"Nikhila Mathew, Arvindh Somanathan, Abha Tirpude, Anupama M Pillai, Pabitra Mondal, Tanvir Arfin","doi":"10.1039/d4ay01767f","DOIUrl":"https://doi.org/10.1039/d4ay01767f","url":null,"abstract":"<p><p>Dioxins rank among the most hazardous persistent organic pollutants, presenting a serious threat due to their long environmental lifespan and capacity for bioaccumulation. This comprehensive review delves into the historical, chemical, and toxicological aspects of dioxins, spotlighting significant incidents such as the Seveso disaster and the repercussions of Agent Orange. The review offers a thorough analysis of the sources of dioxin formation, encompassing natural occurrences like volcanic eruptions and wildfires, alongside man-made activities such as industrial combustion and waste incineration. It examines regional variations in dioxin contamination, revealing air concentrations that can range from less than 0.01 pg TEQ per m³ in remote regions to as high as 2 pg TEQ per m³ in urban environments. With global dioxin emissions estimated at around 97.0 kg TEQ per year, Asia and Africa emerge as the highest emitters among the continents, with the total global dioxin release approximately at 100.4 kg TEQ annually. Dioxin emissions per capita show stark contrasts across six continents, from 10.77 g TEQ per capita in Europe to a concerning 71.66 g TEQ per capita in Oceania. Furthermore, the concentration of dioxin compounds produced during combustion varies significantly, ranging from 15 to 555 ng m^-2. While dioxin emission regulations are intricate and differ globally, most nations require that concentrations remain below one ng m^-2. Globally, dioxin production is estimated at 17 226 kilograms annually, equating to about 287 kilograms in toxic equivalent (TEQ). This review critically examines the severe health implications of dioxins, which include carcinogenic effects, endocrine disruption, and immunotoxicity. Innovative remediation strategies, such as using nanomaterials for adsorption and advanced oxidation processes, are identified as promising pathways to tackle this pressing issue. Ultimately, this review underscores the necessity for enhanced monitoring systems and comprehensive policy frameworks to facilitate sustainable dioxin management and regulatory compliance. Taking decisive action is vital to protect public health and the environment from the ongoing threat posed by dioxins.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual recycling signal amplification strategy based on autocatalytic entropy-driven circuit and DNAzyme for colorimetric detection of platelet-derived growth factor-BB.","authors":"Yuxin Cheng, Hongli Shi, Min Li, Jing Yuan, Cuisong Zhou, Jianyuan Dai","doi":"10.1039/d4ay02128b","DOIUrl":"https://doi.org/10.1039/d4ay02128b","url":null,"abstract":"<p><p>Platelet-derived growth factor-BB (PDGF-BB), an important protein biomarker, is closely associated with tumorigenesis. Therefore, it is important to develop a simple and sensitive method to detect PDGF-BB. Herein, we developed a dual recycling signal amplification strategy for colorimetric and sensitive detection of PDGF-BB using a PDGF-BB specific aptamer. In the presence of PDGF-BB, the first entropy-driven circuit (EDC) reaction cycle was triggered for colorimetric signal amplification. Meanwhile, a catalytic Mg²⁺-dependent DNAzyme was formed on one side of the EDC product, which could cleave its substrate and generate numerous \"mimic trigger\" DNA products. Then, an autocatalytic EDC (AEDC) reaction was triggered by the \"mimic trigger\" DNA, and the signal amplification efficiency of this colorimetric assay was significantly improved. This AEDC- and DNAzyme-based colorimetric assay showed a good linear range from 5.0 to 100 nM for PDGF-BB and the limit of detection was calculated to be 2.2 nM. In addition, PDGF-BB has been quantitatively detected in human serum samples. This colorimetric assay can be easily extended to detect different protein biomarkers by using other recognition elements (aptamers) and shows great potential for clinical diagnosis and prognosis.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of 36 non-phthalate plasticizers with the QuEChERS method in milk powder by gas chromatography coupled with quadrupole/orbitrap high-resolution mass spectrometry.","authors":"Jia-Jia Li, Lu Kou, Jun Li, Pei-Yu Shi, Tian-Rong Guo, Quan-Wei Xiao, Yi Wang, Min Zhang, Wen-Lin Wu","doi":"10.1039/d4ay02223h","DOIUrl":"https://doi.org/10.1039/d4ay02223h","url":null,"abstract":"<p><p>A method involving gas chromatography coupled with quadrupole/orbitrap high-resolution mass spectrometry (GC-Q/Orbitrap HRMS) with the QuEChERS method was developed to analyze 36 non-phthalate plasticizers in milk powder products. The samples were dissolved in 20% NaCl, extracted with acetonitrile, and purified using silica, PSA, and C₁₈. The results showed the excellent linear relationship of the calibration curves of 36 non-phthalate plasticizers in the range of 10-1000 ng mL^-1, with correlation coefficients (<i>r</i>) not less than 0.995. The limit of detection (LODs) of 36 non-phthalate plasticizers ranged from 0.02 to 0.04 mg kg^-1, and the limit of quantification (LOQs) ranged from 0.04 to 0.08 mg kg^-1. The recovery rates were between 64.1% and 116.5%, with the relative standard deviation less than 10%. The proposed method was applied to the detection and analysis of 36 non-phthalate plasticizers in 50 milk powder samples. The findings indicate that this highly sensitive, accurate, and high-throughput method is suitable for the rapid screening and identification of 36 non-phthalate plasticizers in milk powder products.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}