Springer最新文献_第9页

Genetic Correlation of HBB, HFE and HAMP Genes to Endocrinal Complications in Egyptian Beta Thalassemia Major Patients. 埃及重型β地中海贫血患者的 HBB、HFE 和 HAMP 基因与内分泌并发症的遗传相关性。

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-08-01 Epub Date: 2024-07-02 DOI: 10.1007/s10528-024-10868-5

Mona F Sokkar, Mona Hamdy, Mohamed B Taher, Heba El-Sayed, Eman Abdelmotaleb Bayomi, Khalda S Amr, Ghada Y El-Kamah

{"title":"Genetic Correlation of HBB, HFE and HAMP Genes to Endocrinal Complications in Egyptian Beta Thalassemia Major Patients.","authors":"Mona F Sokkar, Mona Hamdy, Mohamed B Taher, Heba El-Sayed, Eman Abdelmotaleb Bayomi, Khalda S Amr, Ghada Y El-Kamah","doi":"10.1007/s10528-024-10868-5","DOIUrl":"10.1007/s10528-024-10868-5","url":null,"abstract":"Iron loading is regarded as the primary cause of endocrine abnormalities in thalassemia major patients. Thus, the purpose of the current research was to explore the impact of thalassemia genotypes, hepcidin antimicrobial peptide (HAMP) and hereditary hemochromatosis (HFE) gene variants, and hepcidin expression on serum ferritin and endocrinal complications in thalassemia patients. The study comprised fifty beta-thalassemia cases and fifty age- and sex-matched controls. Genotyping of the Beta-globin gene (HBB), HAMP, and exon 2 of the HFE gene was performed using Sanger sequencing. C282Y (c.845G > A) variant of the HFE gene was determined by PCR-RFLP. Hepcidin mRNA expression was assessed by qRT-PCR. Biochemical and hormonal studies were done for all patients. Hypogonadism and short stature were found in 56% and 20% of the investigated cases, respectively. Molecular studies reported a statistically higher frequency of the HAMP variant c.-582A > G in thalassemic patients than controls. Significant downregulation of hepcidin expression was found in cases compared to healthy subjects that was significantly associated with short stature. Considering the thalassemia alleles, the IVSI.1G > A (β0) allele was statistically related to hypogonadism. Our results proposed that thalassemia genotypes and downregulated hepcidin expression were the potential risk factors for endocrinopathies in our cases. We also demonstrated an increased incidence of the HAMP promoter variant c.- 582A > G that might have a role in the pathogenesis of iron overload in thalassemic cases. Significant downregulation of hepcidin expression, that contributes to increased iron burden, could be used as a future therapeutic target in these patients.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":"3267-3286"},"PeriodicalIF":2.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolutionary History of the DD41D Family of Tc1/Mariner Transposons in Two Mayetiola Species. 两个马耶地拉物种中 Tc1/Mariner 转座子 DD41D 家族的进化史

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-08-01 Epub Date: 2024-08-08 DOI: 10.1007/s10528-024-10898-z

Wiem Ben Amara, Salma Djebbi, Maha Mezghani Khemakhem

{"title":"Evolutionary History of the DD41D Family of Tc1/Mariner Transposons in Two Mayetiola Species.","authors":"Wiem Ben Amara, Salma Djebbi, Maha Mezghani Khemakhem","doi":"10.1007/s10528-024-10898-z","DOIUrl":"10.1007/s10528-024-10898-z","url":null,"abstract":"Tc1/mariner elements are ubiquitous in eukaryotic genomes including insects. They are diverse and divided into families and sub-families. The DD34D family including mauritiana and irritans subfamilies have already been identified in two closely related species of Cecidomyiids M. destructor and M. hordei. In the current study the de novo and similarity-based methods allowed the identification for the first time of seven consensuses in M. destructor and two consensuses in M. hordei belonging to DD41D family whereas the in vitro method allowed the amplification of two and three elements in these two species respectively. Most of identified elements accumulated different mutations and long deletions spanning the N-terminal region of the transposase. Phylogenetic analyses showed that the DD41D elements were clustered in two groups belonging to rosa and Long-TIR subfamilies. The age estimation of the last transposition events of the identified Tc1/mariner elements in M. destructor showed different evolutionary histories. Indeed, irritans elements have oscillated between periods of silencing and reappearance while rosa and mauritiana elements have shown regular activity with large recent bursts. The study of insertion sites showed that they are mostly intronic and that some recently transposed elements occurred in genes linked to putative DNA-binding domains and enzymes involved in metabolic chains. Thus, this study gave evidence of the existence of DD41D family in two Mayetiola species and an insight on their evolutionary history.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":"3694-3716"},"PeriodicalIF":2.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141905488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reintroducing threatened pine-associated fungal species in boreal forests. 在北方森林重新引入受威胁的松树相关真菌物种。

IF 2.7 3区环境科学与生态学

Environmental Management Pub Date : 2025-08-01 Epub Date: 2025-04-26 DOI: 10.1007/s00267-025-02166-6

Joette Crosier, Reijo Penttilä, Otto Miettinen, Brendan Furneaux, Jorma Pennanen, Leena Hamberg

{"title":"Reintroducing threatened pine-associated fungal species in boreal forests.","authors":"Joette Crosier, Reijo Penttilä, Otto Miettinen, Brendan Furneaux, Jorma Pennanen, Leena Hamberg","doi":"10.1007/s00267-025-02166-6","DOIUrl":"10.1007/s00267-025-02166-6","url":null,"abstract":"Many species of wood-inhabiting fungi, particularly in the boreal forests of Nordic countries, face significant extinction risks. The historical impact of commercial forestry has led to fragmented old-growth forests, meaning that species lost from these areas may not naturally return to newly protected habitats. This study investigates the potential of inoculation as a management tool to aid the reintroduction of threatened fungal species. Specifically, we tested whether red-listed fungal species associated with dead pine wood could successfully establish in inoculated pine logs and identified factors influencing inoculation success. We cultured mycelium from five target species (Anthoporia albobrunnea, Antrodia crassa, Antrodia infirma, Crustoderma corneum, Dichomitus squalens) and inoculated pine logs in selected forests, monitoring log characteristics and conducting DNA analysis of the fungal community before and after inoculation. Our findings demonstrate that all species successfully established in at least some of the inoculated logs (28-60% success), with variable effects of log characteristics on fungal abundance. Additionally, the presence of certain fungi correlated with the success of the inoculated species. These results suggest that inoculation can be a promising method for aiding the recovery of threatened wood-inhabiting fungi in appropriate forest habitats. Long-term monitoring is necessary to assess fruiting success and population sustainability, while further exploration of alternative techniques could enhance the effectiveness of reintroduction efforts in forest management practices.","PeriodicalId":543,"journal":{"name":"Environmental Management","volume":" ","pages":"1938-1949"},"PeriodicalIF":2.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12259776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Examining Capacity-Outcome Relationships to Develop an Environmental Stewardship Capacity Index in New York City. 检视能力-结果关系以发展纽约市环境管理能力指数。

IF 2.7 3区环境科学与生态学

Environmental Management Pub Date : 2025-08-01 Epub Date: 2025-04-02 DOI: 10.1007/s00267-025-02145-x

Michelle L Johnson, Lindsay K Campbell, Erika S Svendsen

{"title":"Examining Capacity-Outcome Relationships to Develop an Environmental Stewardship Capacity Index in New York City.","authors":"Michelle L Johnson, Lindsay K Campbell, Erika S Svendsen","doi":"10.1007/s00267-025-02145-x","DOIUrl":"10.1007/s00267-025-02145-x","url":null,"abstract":"Civic capacity to care for the urban environment is a key factor in urban sustainability efforts. Civic environmental stewardship groups play critical roles in solving governance challenges associated with sustainability, serving as brokers, acting as stewards, advocating for policies, and being repositories of social-ecological memory. Understanding the existing capacity of such groups to create social-ecological outcomes across the urban landscape enables better planning and implementation of equitable nature-based solutions. In this paper, we draw upon existing frameworks of community capacity and environmental stewardship to operationalize an empirical, organizational stewardship capacity index at a neighborhood scale. We applied survey-based civic environmental stewardship mapping data from the New York City 2017 Stewardship Mapping and Assessment Project (STEW-MAP) to create this index. We evaluated stewardship capacity variables against perceived social and environmental outcome measures collected by groups in the STEW-MAP survey to develop the stewardship capacity index. We find that neighborhoods where more groups exist, where more time is spent on stewardship, and where more types of stewardship actions are supported have better perceived Cumulative outcomes. Our results highlight the known role of networks in community capacity and reveal the roles of diversity of stewardship actions (Stewardship types) and level of effort (Time spent on stewardship) in achieving outcomes. The result of our analyses is a mapped index of stewardship capacity, which visualizes the in current capacities at a neighborhood level and identifies future opportunities for capacity building. With a focus on transparency, this index can be applied by managers, public and civic alike, as they seek to support and increase civic capacity to care for the environment.","PeriodicalId":543,"journal":{"name":"Environmental Management","volume":" ","pages":"2169-2181"},"PeriodicalIF":2.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143770864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring of miR-155-5p, miR-181b-5p, and miR-454-3p Expressions in Circulating Cell-Free RNA: Insights from Peripheral Blood of Uveal Malignant Melanoma Patients. 探索循环细胞游离 RNA 中 miR-155-5p、miR-181b-5p 和 miR-454-3p 的表达：葡萄膜恶性黑色素瘤患者外周血的启示。

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-08-01 Epub Date: 2024-06-24 DOI: 10.1007/s10528-024-10849-8

Hassani Masoumeh, Doğan Tunay, Ödemiş Akdeniz Demet, Tuncer Samuray, Yazıcı Hülya

{"title":"Exploring of miR-155-5p, miR-181b-5p, and miR-454-3p Expressions in Circulating Cell-Free RNA: Insights from Peripheral Blood of Uveal Malignant Melanoma Patients.","authors":"Hassani Masoumeh, Doğan Tunay, Ödemiş Akdeniz Demet, Tuncer Samuray, Yazıcı Hülya","doi":"10.1007/s10528-024-10849-8","DOIUrl":"10.1007/s10528-024-10849-8","url":null,"abstract":"The identification of novel non-invasive biomarkers is imperative for the early diagnosis and monitoring of malignant melanoma. The objective of this study is to examine the expression levels of miR-155-5p, miR-181b-5p, and miR-454-3p in circulating cell-free RNA obtained from plasma samples of the 72 uveal malignant melanoma patients and to compare these levels with those of 72 healthy controls. The analysis showed that the expression level of the miR-181b-5p has increased 9.25 fold, and expression level of miR-155-5p has increased 6.67 fold, and miR-454-3p expression level has increased 4.14 fold in the patient group compared with the levels in the healthy control group (p = 0.005). It was found that the high expression levels of the three miRNAs were statistically significant in patients compared with in the healthy control group. The statistical evaluations between miRNA expression levels and clinical data showed that miR-155-5p had significant association with radiation therapy (p = 0.040), and miR-454-3p showed a significant association with smoking and alcohol use respectively (p = 0.009, and p = 0.026). The significantly elevated expression levels of miR-181b-5p, miR-155-5p, and miR-454-3p in the circulating cell-free RNA of plasma from uveal melanoma patients, in comparison to those in the healthy control group, suggest the potential usefulness of these biomarkers for both early diagnosis and disease monitoring. However, more extensive and future studies are needed to use these molecules in early diagnosis and disease monitoring.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":"3187-3205"},"PeriodicalIF":2.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12271267/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141445165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A protocol for heat treatment of four types of clay minerals used in French pelotherapy facilities. 法国理疗设施中使用的四种粘土矿物的热处理规程。

IF 3 3区地球科学

International Journal of Biometeorology Pub Date : 2025-08-01 Epub Date: 2025-06-02 DOI: 10.1007/s00484-025-02938-z

Sébastien Labarthe, Karine Dubourg, Frédéric Bauduer

{"title":"A protocol for heat treatment of four types of clay minerals used in French pelotherapy facilities.","authors":"Sébastien Labarthe, Karine Dubourg, Frédéric Bauduer","doi":"10.1007/s00484-025-02938-z","DOIUrl":"10.1007/s00484-025-02938-z","url":null,"abstract":"In France, pelotherapy is mainly performed through the application of mud-packs that are conserved in heating cabinets and deposed on patient skin at a temperature between 40 and 45 °C. Repeated contacts with persons and storage conditions for their individual re-use are associated with microbiological risks. Currently, there are no quality standards or guidelines for the sanitary monitoring of this treatment. However, we used here the most stringent requirement proposed by the French National Academy of Medicine which recommends the heating of therapeutic muds up to 70 °C for at least 30 min in order to eliminate SARS-CoV-2 that may concentrate in sediments and is more resistant to heat inactivation than usual bacteria. To achieve this objective, operators lack information for scheduling a protocol regarding heat treatment duration and temperature because these parameters may vary according to the type of heating cabinet and composition/properties of extractive clays/mineral water. A series of heating/cooling scenarios using the most popular heating cabinet existing in French medical spas on four main types of extractive clays was tested. The target thermal conditions have been met with this chamber when scheduling the following data: 80 °C during 1h45 for a montmorillonite, illite and kaolinite mix, 2 h for a Ca-Montmorillonite and Na-Montmorillonite, and 1h30 for kaolinite. Due to very slow cooling kinetics, cycles duration was important (from 17 to 33 h), rendering necessary a reorganization in the daily scheduling of the procedure for therapeutic sessions. In addition, for application of these muds to the patient at 45 °C, a setting maintenance temperature at 50 °C (or at 45 °C for a 40 °C application temperature) is to be recommended. After validation by direct microbiological tests this protocol could be proposed as a methodological reference to the French medical spas using this type of heating material for pelotherapy.","PeriodicalId":588,"journal":{"name":"International Journal of Biometeorology","volume":" ","pages":"1919-1927"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of endogenous SUMOylation sites by click chemistry-based proteomics. 内源性sumo酰化位点的蛋白质组学表征。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-08-01 Epub Date: 2025-06-16 DOI: 10.1007/s00216-025-05957-2

Ke Ma, Sixian Chen, Jun Zhang, Xinglong Jia, Rufeng Fan, Mingjun Li, Li Dong, Minjia Tan, Wensi Zhao, Dong Xie

{"title":"Characterization of endogenous SUMOylation sites by click chemistry-based proteomics.","authors":"Ke Ma, Sixian Chen, Jun Zhang, Xinglong Jia, Rufeng Fan, Mingjun Li, Li Dong, Minjia Tan, Wensi Zhao, Dong Xie","doi":"10.1007/s00216-025-05957-2","DOIUrl":"10.1007/s00216-025-05957-2","url":null,"abstract":"SUMOylation, an essential ubiquitin-like modification in eukaryotes, plays vital roles in both physiological and pathological regulation, positioning it as a promising therapeutic target. However, the low abundance of SUMOylation and the high enzymatic activity of sentrin/SUMO-specific proteases (SENPs) complicate the identification of endogenous sites. In this study, we integrated click chemistry, acid cleavage, and SUMOylated peptide enrichment into the workflow and developed a promising methodology for system-wide identification of SUMOylation sites. In total, we identified 962 endogenous SUMOylation sites in HEK293T cells under heat shock conditions, which showed good complementarity with previous studies. Our approach uncovered 105 potentially new sites, including SSRP1-K248/K319/K612, DHX9-K806, and ILF3-K241, which showed high conservation and were located in functionally important domains. The overlap between SUMOylation sites and the known ubiquitination or acetylation sites suggested the potential PTM crosstalks. KEGG analysis further suggested SUMOylated proteins were associated with carbon metabolism and biosynthesis of amino acids pathways. Collectively, this study provides a valuable tool for systematically identifying SUMOylation sites, advancing further biological understanding of their dynamic regulatory networks and pathophysiological mechanisms.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"4419-4433"},"PeriodicalIF":3.8,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and characterization of angiotensin I certified reference materials. 血管紧张素I标准物质的研制与表征。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-08-01 Epub Date: 2025-06-19 DOI: 10.1007/s00216-025-05955-4

Fanyu Deng, Ruiqi Wang, Kaifa Liu, Liqing Wu, Rui Su, Yahui Liu

{"title":"Development and characterization of angiotensin I certified reference materials.","authors":"Fanyu Deng, Ruiqi Wang, Kaifa Liu, Liqing Wu, Rui Su, Yahui Liu","doi":"10.1007/s00216-025-05955-4","DOIUrl":"10.1007/s00216-025-05955-4","url":null,"abstract":"Angiotensin I (ANGI) plays an important role in regulating blood pressure and maintaining homeostasis; the accurate analysis of its levels is beneficial in the clinical diagnosis and treatment of diseases. Despite the existence of several detection techniques for ANGI, a \"yardstick\" to evaluate the results of different detection techniques is still lacking. To ensure the reliability of ANGI tests along with the accuracy and comparability of the analytical results, natural and isotope-labeled ANGI certified reference materials were characterized, and their purities were assessed by mass balance and quantitative nuclear magnetic resonance methods. The moisture content of the samples was determined using the Karl Fischer method, anion content was measured via ion chromatography, and inorganic elements were detected via inductively coupled plasma mass spectrometry. In the quantitative nuclear magnetic resonance method, maleic acid was used as a quantitative internal standard, and the final values of natural and isotope-labeled ANGI were determined on the basis of their quantitative peaks. The final purity was verified by isotope dilution mass spectrometry. The natural and labeled ANGI purities were (0.8511 ± 0.041) g/g and (0.8696 ± 0.042) g/g, respectively. Furthermore, the abundance of double-labeled ANGI-which refers to the labeling rate of 13C and 15N in labeled ANGI-was determined via high-resolution liquid-mass spectrometry, affording 98.98% 13C and 99.43% 15N. This study establishes high-purity natural and isotope-labeled ANGI reference materials, ensuring accurate, comparable measurements for clinical diagnostics and biomarker research. It also supports method validation, quality control, and interlaboratory comparisons through advanced analytical techniques.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"4395-4406"},"PeriodicalIF":3.8,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification of poly(ethylene terephthalate) in environmental samples after methanolysis via gas chromatography-mass spectrometry. 气相色谱-质谱联用法测定甲醇分解后环境样品中的聚对苯二甲酸乙酯。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-08-01 Epub Date: 2025-07-09 DOI: 10.1007/s00216-025-05963-4

Tim Lauschke, Ann-Christin Merfels, Thomas A Ternes, Georg Dierkes

{"title":"Quantification of poly(ethylene terephthalate) in environmental samples after methanolysis via gas chromatography-mass spectrometry.","authors":"Tim Lauschke, Ann-Christin Merfels, Thomas A Ternes, Georg Dierkes","doi":"10.1007/s00216-025-05963-4","DOIUrl":"10.1007/s00216-025-05963-4","url":null,"abstract":"Quantification of the polyester poly(ethylene terephthalate) (PET) in environmental samples is a particular challenge. Due to strong matrix effects by inorganic compounds, thermoanalytical methods are not recommendable for a precise quantification of PET in complex environmental matrices. It was shown that depolymerization followed by determination of chemolysis products is a good alternative. In this study, we developed a quantification method for PET based on methanolysis to terephthalic acid dimethyl ester using sodium methoxide as a catalyst, and subsequent determination via GC-MS. With poly(ethylene terephthalate-d4), we introduce a new internal standard covering the whole analytical process. Satisfactory detection and quantifications limits (1 and 4 µg g-1) as well as recoveries of 87-117% were achieved. Tests with various PET-free natural compounds exhibited no interfering matrix effects. The newly developed method was applied for MP quantification in a variety of environmental samples such as sediments, sewage sludge, indoor dust, and water. In all these matrices, PET was present. Highest concentrations were detected in indoor dust with up to 57 mg/g. In bottled water, PET concentrations were detected as high as 463 ng/L. The described depolymerization method offers a straightforward approach for a reliable quantification of PET in complex environmental matrices suitable for routine analysis.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"4461-4468"},"PeriodicalIF":3.8,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uncertainty factors and relative response factors: correcting detection and quantitation bias in extractables and leachables studies. 不确定因素和相对响应因素：在可提取物和可浸物研究中纠正检测和定量偏差。

IF 3.8 2区化学

Analytical and Bioanalytical Chemistry Pub Date : 2025-08-01 Epub Date: 2025-07-15 DOI: 10.1007/s00216-025-05946-5

Marco Giulio Rozio, Davide Angelini, Simone Carrara

{"title":"Uncertainty factors and relative response factors: correcting detection and quantitation bias in extractables and leachables studies.","authors":"Marco Giulio Rozio, Davide Angelini, Simone Carrara","doi":"10.1007/s00216-025-05946-5","DOIUrl":"10.1007/s00216-025-05946-5","url":null,"abstract":"The transfer of chemicals from packaging or medical devices to drug formulations, known as extractables and leachables (E&L) release, can affect drug strength and safety. These released substances must be monitored and assessed through toxicological evaluation. Identifying and quantifying analytes above a specific analytical evaluation threshold (AET) is crucial, but variability in response factors (RFs) complicates accurate detection, leading to potential errors in quantitation. An uncertainty factor (UF) can partially correct this, though it is limited by RF variability, and a multidetector approach improves characterization but does not fully resolve quantitation bias. The RRFlow model proposed in this study offers a solution by determining E&L concentrations without real-time reference standards analysis. It involves identity confirmation, RRF validation, and applies an average corrective factor (RRFi). A numerical simulation benchmark (NSB) is used to compare different scenarios, such as varying UF values, RRFlow application, and fixed rescaling factors. The benchmark assigns concentration values to model compounds with different response factors, iterating the process to evaluate the number of false positive and negative errors. The numerical simulations show that RRFlow reduces detection bias and outperforms UF-based methods, mitigating false positives and negatives.","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":"4331-4349"},"PeriodicalIF":3.8,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0