Shijie Wang, Yan Cheng, Zhelin Liu, Xiangting Dong, Bo Zhao
{"title":"二茂铁功能化铟氧簇纳米酶检测过氧化氢和抗坏血酸。","authors":"Shijie Wang, Yan Cheng, Zhelin Liu, Xiangting Dong, Bo Zhao","doi":"10.1007/s00216-025-06078-6","DOIUrl":null,"url":null,"abstract":"<div><p>Nanozymes are a class of nanomaterials with intrinsic enzyme-like properties, which overcome the limitations of natural enzymes, such as poor stability, high cost, and difficulty in storage, thus achieving rapid development. Herein, the enzyme-like activity of ferrocene-functionalized indium-oxo cluster (TPP<sup>+</sup>)[In<sub>7</sub>FcDCA<sub>6</sub>(µ<sub>4</sub>-O<sup>2−</sup>)<sub>3</sub>(µ<sub>3</sub>-OCH<sub>3</sub>)(Cl<sup>−</sup>)<sub>3</sub>], named [In<sub>7</sub>Fc<sub>6</sub>], was analyzed. The obtained [In<sub>7</sub>Fc<sub>6</sub>] cluster has good peroxidase-like activity, which can catalyze the reaction of 3,3′,5,5′-tetramethylbenzidine (TMB), <i>o</i>-phenylenediamine (OPD), and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) with H<sub>2</sub>O<sub>2</sub>. Therefore, based on the peroxidase-like activity of [In<sub>7</sub>Fc<sub>6</sub>], a colorimetric sensor for the detection of H<sub>2</sub>O<sub>2</sub> was developed, which could detect H<sub>2</sub>O<sub>2</sub> in the concentration range of 1–170 μM, and the detection limit was 0.24 μM. In addition, we also established a colorimetric sensor for detecting ascorbic acid (AA) in the range of 3–130 μM with a detection limit of 0.81 μM. The practicability of the established colorimetric sensor was also verified in cells and serum. This research opens up new horizons for exploring new application possibilities for clusters in biomolecular detection.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 26","pages":"5847 - 5858"},"PeriodicalIF":3.8000,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Ferrocene-functionalized indium-oxo cluster nanozyme for the detection of hydrogen peroxide and ascorbic acid\",\"authors\":\"Shijie Wang, Yan Cheng, Zhelin Liu, Xiangting Dong, Bo Zhao\",\"doi\":\"10.1007/s00216-025-06078-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Nanozymes are a class of nanomaterials with intrinsic enzyme-like properties, which overcome the limitations of natural enzymes, such as poor stability, high cost, and difficulty in storage, thus achieving rapid development. Herein, the enzyme-like activity of ferrocene-functionalized indium-oxo cluster (TPP<sup>+</sup>)[In<sub>7</sub>FcDCA<sub>6</sub>(µ<sub>4</sub>-O<sup>2−</sup>)<sub>3</sub>(µ<sub>3</sub>-OCH<sub>3</sub>)(Cl<sup>−</sup>)<sub>3</sub>], named [In<sub>7</sub>Fc<sub>6</sub>], was analyzed. The obtained [In<sub>7</sub>Fc<sub>6</sub>] cluster has good peroxidase-like activity, which can catalyze the reaction of 3,3′,5,5′-tetramethylbenzidine (TMB), <i>o</i>-phenylenediamine (OPD), and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) with H<sub>2</sub>O<sub>2</sub>. Therefore, based on the peroxidase-like activity of [In<sub>7</sub>Fc<sub>6</sub>], a colorimetric sensor for the detection of H<sub>2</sub>O<sub>2</sub> was developed, which could detect H<sub>2</sub>O<sub>2</sub> in the concentration range of 1–170 μM, and the detection limit was 0.24 μM. In addition, we also established a colorimetric sensor for detecting ascorbic acid (AA) in the range of 3–130 μM with a detection limit of 0.81 μM. The practicability of the established colorimetric sensor was also verified in cells and serum. This research opens up new horizons for exploring new application possibilities for clusters in biomolecular detection.</p><h3>Graphical Abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":462,\"journal\":{\"name\":\"Analytical and Bioanalytical Chemistry\",\"volume\":\"417 26\",\"pages\":\"5847 - 5858\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2025-08-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical and Bioanalytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00216-025-06078-6\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical and Bioanalytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00216-025-06078-6","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Ferrocene-functionalized indium-oxo cluster nanozyme for the detection of hydrogen peroxide and ascorbic acid
Nanozymes are a class of nanomaterials with intrinsic enzyme-like properties, which overcome the limitations of natural enzymes, such as poor stability, high cost, and difficulty in storage, thus achieving rapid development. Herein, the enzyme-like activity of ferrocene-functionalized indium-oxo cluster (TPP+)[In7FcDCA6(µ4-O2−)3(µ3-OCH3)(Cl−)3], named [In7Fc6], was analyzed. The obtained [In7Fc6] cluster has good peroxidase-like activity, which can catalyze the reaction of 3,3′,5,5′-tetramethylbenzidine (TMB), o-phenylenediamine (OPD), and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) with H2O2. Therefore, based on the peroxidase-like activity of [In7Fc6], a colorimetric sensor for the detection of H2O2 was developed, which could detect H2O2 in the concentration range of 1–170 μM, and the detection limit was 0.24 μM. In addition, we also established a colorimetric sensor for detecting ascorbic acid (AA) in the range of 3–130 μM with a detection limit of 0.81 μM. The practicability of the established colorimetric sensor was also verified in cells and serum. This research opens up new horizons for exploring new application possibilities for clusters in biomolecular detection.
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Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
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