二茂铁功能化铟氧簇纳米酶检测过氧化氢和抗坏血酸。

IF 3.8 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS
Shijie Wang, Yan Cheng, Zhelin Liu, Xiangting Dong, Bo Zhao
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引用次数: 0

摘要

纳米酶是一类具有酶样特性的纳米材料,它克服了天然酶稳定性差、成本高、储存困难等缺点,从而获得了快速发展。本文分析了二茂铁功能化铟氧团簇(TPP+)[In7FcDCA6(µ4-O2-)3(µ3- och3)(Cl-)3],命名为[In7Fc6]的酶样活性。所得的[In7Fc6]簇具有良好的过氧化物酶样活性,可催化3,3',5,5'-四甲基联苯胺(TMB)、邻苯二胺(OPD)和2,2'-氮基-双(3-乙基苯并噻唑-6-磺酸)(ABTS)与H2O2的反应。因此,基于[In7Fc6]的过氧化物酶样活性,研制了一种检测H2O2的比色传感器,可检测1 ~ 170 μM浓度范围内的H2O2,检出限为0.24 μM。此外,我们还建立了抗坏血酸(AA)的比色传感器,检测限为0.81 μM,检测范围为3 ~ 130 μM。所建立的比色传感器的实用性也在细胞和血清中得到验证。本研究为探索簇在生物分子检测中的新应用可能性开辟了新的视野。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ferrocene-functionalized indium-oxo cluster nanozyme for the detection of hydrogen peroxide and ascorbic acid.

Nanozymes are a class of nanomaterials with intrinsic enzyme-like properties, which overcome the limitations of natural enzymes, such as poor stability, high cost, and difficulty in storage, thus achieving rapid development. Herein, the enzyme-like activity of ferrocene-functionalized indium-oxo cluster (TPP+)[In7FcDCA64-O2-)33-OCH3)(Cl-)3], named [In7Fc6], was analyzed. The obtained [In7Fc6] cluster has good peroxidase-like activity, which can catalyze the reaction of 3,3',5,5'-tetramethylbenzidine (TMB), o-phenylenediamine (OPD), and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) with H2O2. Therefore, based on the peroxidase-like activity of [In7Fc6], a colorimetric sensor for the detection of H2O2 was developed, which could detect H2O2 in the concentration range of 1-170 μM, and the detection limit was 0.24 μM. In addition, we also established a colorimetric sensor for detecting ascorbic acid (AA) in the range of 3-130 μM with a detection limit of 0.81 μM. The practicability of the established colorimetric sensor was also verified in cells and serum. This research opens up new horizons for exploring new application possibilities for clusters in biomolecular detection.

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来源期刊
CiteScore
8.00
自引率
4.70%
发文量
638
审稿时长
2.1 months
期刊介绍: Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
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