Rapid Communications in Mass Spectrometry最新文献

{"title":"Ecological Insights Into the Extinct Korean Sea Lion (Zalophus japonicus) in Korea Based on Stable Isotope Analysis of Bone Collagen","authors":"Yoon Ji Lee,&nbsp;Myung Joon Kim,&nbsp;Ji Seong Kim,&nbsp;Seok Nam Kwak,&nbsp;Sang Heon Lee","doi":"10.1002/rcm.10140","DOIUrl":"10.1002/rcm.10140","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>The Japanese sea lion (<i>Zalophus japonicus</i>), once abundant in Japan, Korea, and Russia, went extinct by the mid-20th century due to hunting and environmental change. This study examines the diet and ecological role of Korean <i>Z. japonicus</i> using stable carbon and nitrogen isotope analysis of bone collagen from archaeological and historical contexts.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Skeletal remains of the extinct Korean sea lion (<i>Z. japonicus</i>) were excavated from Gajae-gull, Ulleungdo, in 2021. A rib bone was analyzed for radiocarbon dating using AMS and calibrated with the Marine20 curve, applying a ΔR correction. Stable isotope analysis of extracted bone collagen (δ¹³C, δ¹⁵N) was performed via EA-IRMS. Isotopic niches of <i>Z. japonicus</i> and modern marine mammals were compared using SIBER in R, with ANOVA and post hoc tests.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Radiocarbon dating of a rib from Gajae-gull, Ulleungdo, indicated burial between 1548 and 1952 cal <span>ad</span>, representing the most recent evidence of <i>Z. japonicus</i> in Korea. Stable isotope analysis revealed dietary shifts from high δ¹⁵N values in the Chulmun periods, indicating consumption of large fish and cephalopods, to lower δ¹⁵N and higher δ¹³C values in the Mumun and historic periods, indicating reliance on coastal mid-trophic prey. Comparisons with modern marine mammals showed clear isotopic niche partitioning.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>These findings underscore the dietary flexibility of Korean <i>Z. japonicus</i> and its shifting ecological role in response to changing prey availability and environmental conditions. Future research incorporating additional samples and diverse isotopic markers will enhance paleoecological reconstructions of this extinct species and its historical marine ecosystems.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 24","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/rcm.10140","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultratrace Determination of Cd (II) in Water, Salt, Environmental, Alloy, and Biological Samples by Liquid–Liquid Extraction Followed by Inductively Coupled Plasma Mass Spectrometry Technique","authors":"Chandramauly Sharma,&nbsp;Kuldeep Sharma,&nbsp;Bignesh Thakur,&nbsp;Aniket Dixit","doi":"10.1002/rcm.10150","DOIUrl":"https://doi.org/10.1002/rcm.10150","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>Cadmium is a highly toxic heavy metal associated with severe environmental and health risks, making its precise quantification in complex matrices critical. Inductively coupled plasma mass spectrometry (ICP–MS) is one of the most powerful tools for trace metal analysis due to its exceptional sensitivity, selectivity, and multielement capability. However, accurate detection often requires effective preconcentration and matrix elimination strategies.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method</h3>\u0000 \u0000 <p>A liquid–liquid extraction procedure was developed using N,N′-diphenyl sebacoyl dihydroxamic acid (DPSDHA) as a selective complexing ligand for cadmium. The Cd–DPSDHA binary complex formed at pH 9.5 was extracted into dichloromethane, effectively preconcentrating cadmium and minimizing matrix interference prior to ICP–MS measurement. The optimized conditions ensured maximum recovery and stability of the analyte, thereby improving detection limits and analytical precision.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Result</h3>\u0000 \u0000 <p>Coupling the DPSDHA extraction with ICP–MS enabled trace-level determination of cadmium with excellent sensitivity and reproducibility. The method exhibited a wide linear dynamic range, low detection limits, and strong resistance to matrix interferences, ensuring accurate quantification in complex samples. Application to water, alloy, environmental, and biological samples yielded accurate results, which were consistent with certified reference values.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The combination of selective DPSDHA-based extraction with ICP–MS provides a robust, highly sensitive approach for cadmium determination in diverse sample matrices. This methodology enhances detection accuracy by reducing matrix effects, making it well-suited for environmental monitoring, industrial quality control, and biological studies where trace-level cadmium analysis is essential.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanostructure-Dependent Signal Intensity in Through-Hole Porous Alumina Membranes for Mass Spectrometry Imaging","authors":"Masahiro Kotani,&nbsp;Takashi Yanagishita","doi":"10.1002/rcm.10149","DOIUrl":"https://doi.org/10.1002/rcm.10149","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>Matrix-assisted laser desorption/ionization (MALDI) is a widely used analytical technique for measuring high-molecular-weight compounds such as proteins. However, in the low-molecular-weight region, interference peaks derived from the matrix occur. Surface-assisted laser desorption/ionization (SALDI), which is matrix-free, does not generate background noise in the low-molecular-weight region and has the advantages of simple sample preparation and reproducibility. We previously developed an ionization method using an anodic porous alumina membrane (APAM) as a SALDI substrate. In this study, we examined the effects of the surface nanostructural properties of APAMs, such as hole diameter and pitch, on the signal intensity in mass spectrometry (MS) imaging.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>APAMs were fabricated using electrolytes of oxalic, malonic, and malic acids and were evaluated by MS using droplet samples and MS imaging. Droplet samples were applied to the back surface of the APAMs. MS imaging was conducted using 20-μm-thick mouse brain sections to compare the signal-to-noise ratio (SNR) of each APAM.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>APAMs were fabricated with hole diameters (D_h) of 24–419 nm, interhole distances (D_int) of 100–625 nm, and open area ratios (OAR) of 5%–46%. From MS and MS imaging results, signal intensity at the same OAR increased in the order of D_int = 100, 625, and 270 nm, and the condition D_h/D_int = 131/270 nm provided the highest SNR. In addition, APAMs with a D_h of less than 84 nm and an OAR lower than 10% exhibited lower signal intensities.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>We fabricated APAMs under various conditions and identified the processing conditions that provided the highest SNR of SALDI imaging. SALDI imaging using the APAMs fabricated under these high SNR conditions is expected to be applicable in various fields such as materials science and metabolomics, as it does not generate interference peaks in the low-molecular-weight region.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extreme Rainfall Contribution and Isotopic Excursion of Tropical Storm Alberto in Northeastern Mexico","authors":"Odalys J. Ibarra-Alejos,&nbsp;Ricardo Sánchez-Murillo,&nbsp;Rene Ventura-Houle,&nbsp;Oscar Guevara Mansilla,&nbsp;Tomas Peña-Alonso,&nbsp;Bárbara A. Macías-Hernández,&nbsp;Luis González-Hita,&nbsp;Miguel A. Mejía-González","doi":"10.1002/rcm.10146","DOIUrl":"https://doi.org/10.1002/rcm.10146","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>Tropical cyclones are critical extreme rainfall events that can temporarily mitigate drought impacts in semiarid regions. Despite the well-known North Atlantic hurricane season, the propagation of isotopically distinct rainfall pulses across semiarid landscapes of Mesoamerica is largely unknown.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In June 2024, tropical storm (TS) Alberto made landfall in northeastern Mexico. This study evaluates TS Alberto's monthly and annual rainfall contribution across Tamaulipas and Nuevo León and the isotopic excursions (δ¹⁸O and <i>d</i>-excess) in precipitation and surface water. Rainfall data from 86 stations and high-frequency isotope samples from Ciudad Victoria, Tampico, Chetumal, and the San Marcos River were analyzed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Results show that TS Alberto produced unprecedented rainfall, ranging from 0.22 to 11.41 times the 10-year June average, and up to 123% of the annual average in orographically influenced regions (i.e., variation is based on 86 locations across the region). Reservoir data highlight a rapid hydrological recovery, with dam levels in Tamaulipas and Nuevo León increasing significantly within weeks. Rainfall isotopic composition exhibited a broad spectrum (δ¹⁸O: −15.82‰ to −2.80‰), reflecting varying moisture sources, convective activity, and orographic effects. TS Alberto reproduced nearly the entire annual δ¹⁸O variability within a few hours, underscoring the strong isotopic imprint of extreme events. During TS Alberto's landfall and passage, an inverse trend between δ¹⁸O and precipitation amount was observed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The storm's isotopic signature was traced into surface waters, providing evidence of rapid river response and potentially for aquifer recharge. These findings emphasize the dual hydrological and isotopic role of TCs in modulating water scarcity and improving hydroclimate reconstructions in drought-prone landscapes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/rcm.10146","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping the Metabolic Fate of Suzetrigine in Equine and Camel Models: A Step Toward Reliable Antidoping Detection","authors":"Shino Ann Koshy,&nbsp;Meleparappil Muhammed Ajeebsanu,&nbsp;Abdul Khader Karakka Kal,&nbsp;Michael Benedict Subhahar,&nbsp;Tajudheen K. Karatt,&nbsp;Moses Philip","doi":"10.1002/rcm.10147","DOIUrl":"https://doi.org/10.1002/rcm.10147","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>Effective pain management remains a persistent challenge, with opioids limited by tolerance, dependence, respiratory depression, and misuse. This has created demand for safer, nonaddictive alternatives. Suzetrigine (VX-548, Journavx) is a novel analgesic with promising efficacy, but its potential misuse in competitive racing requires investigation. Limited information is available on its metabolism and detection, emphasizing the need for metabolic characterization to aid antidoping strategies.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In vitro metabolism of suzetrigine was evaluated using equine liver microsomes and homogenized camel liver. Metabolic profiling was carried out using high-resolution LC–HRMS. Fragmentation analysis was performed to assign metabolite structures and characterize biotransformation pathways.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Seven phase I metabolites (M1–M7) were identified, primarily via hydroxylation, methylation, demethylation, detrifluoromethylation, and cleavage of the pyridine-2-carboxamide moiety. One phase II metabolite (M8), formed by sulfation of the demethylated metabolite M5, was detected exclusively with camel liver. Metabolite structures were tentatively assigned based on mass fragmentation data, which showed characteristic neutral losses and diagnostic ions. Comparative analysis revealed that both species shared common phase I pathways, but camel liver demonstrated additional conjugation capacity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study provides the first comprehensive characterization of suzetrigine metabolism in equine and camel liver systems. The results underscore metabolic variations, propose a biotransformation pathway, and offer crucial insights to support the development of antidoping detection strategies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic and Metabolomic Mechanism of Ibuprofen, Chondroitin Sulfate, and Vitamins With Minerals Tablets (21) for Kashin–Beck Disease Treatment","authors":"Jinfeng Wen,&nbsp;Xingxing Deng,&nbsp;Zhengjun Yang,&nbsp;Hui Niu,&nbsp;Qian Zhang,&nbsp;Yijun Zhao,&nbsp;Gaowa Naren,&nbsp;Huan Liu,&nbsp;Feng Zhang,&nbsp;Xiaodong Yang,&nbsp;Cuiyan Wu","doi":"10.1002/rcm.10130","DOIUrl":"10.1002/rcm.10130","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Kashin–Beck disease (KBD) is an osteoarthropathy with no specific drug treatment. This study seeks to uncover the molecular mechanisms of ibuprofen, chondroitin sulfate, and vitamins with minerals tablets (21) in treating KBD.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Integrated transcriptomic and metabolomic analyses were performed on peripheral blood samples collected from four KBD patients before and 1 month after treatment. Differential gene expression was assessed using the DESeq package, while differential metabolites were identified through univariate and multivariate statistical analyses.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>After treatment, 5671 differential genes (|log₂FC| ≥ 1, q &lt; 0.05) were identified, with 4954 upregulated and 717 downregulated. Enriched pathways included PI3K-Akt, MAPK, ECM-receptor interaction, Rap1, and Ras signaling. Untargeted metabolomics analyses revealed five differentially expressed metabolites (VIP ≥ 1, |log₂FC| &gt; 0.58, <i>p</i> &lt; 0.05): L-glutamine, 2-methylpropan-2-amine, epsilon-caprolactam, phosphocholine, and 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine. Metabolite enrichment analysis suggested alterations in amino acid and fatty acid metabolism. Integrated analysis revealed key pathways such as alanine, aspartate, and glutamate metabolism and arginine biosynthesis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The combination of ibuprofen, chondroitin sulfate, and vitamins with minerals tablets (21) may alleviate KBD through modulation of key genes (GDNF, BDNF, CCL2, VEGFA, and PPARG) and glutamine-related metabolic pathways.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 24","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct Metabolic Profiling of Biofluids via Wimshurst Machine–Nanoelectrospray Ionization Mass Spectrometry","authors":"Qiming Kang,&nbsp;Guangming Huang","doi":"10.1002/rcm.10142","DOIUrl":"10.1002/rcm.10142","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>Direct metabolic analysis is crucial for understanding the mechanisms of various diseases. However, it is still difficult to perform direct metabolic analysis of native biofluids for many biological sample types because conventional workflows often necessitate extensive sample pretreatment to mitigate matrix interference from high salt concentrations.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In this study, we developed a novel approach using a Wimshurst Machine–nanoelectrospray ionization (WM-nanoESI) system for direct metabolic analysis of native biofluids. This device achieves direct metabolic analysis due to its sampling method (injecting biofluid directly into nanoESI emitters with an orifice diameter of approximately 10 μm) and the adaptive electricity provided by the Wimshurst machine (preventing tip clogging and extending duration time).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>This method enables qualitative analysis of drugs and metabolites, prevents tip clogging when analyzing PBS and serum, and extends the duration time by approximately 15-fold longer than traditional DC nanoESI does. HeLa and MCF-7 cells were distinguished according to their metabolic profiles.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our results indicate that the WM-nanoESI will offer significant advancements for real-time metabolic monitoring, boost disease understanding, and therapeutic monitoring.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"40 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145190548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Conjugated Octupole–Quadrupole Ion Guide Achieving High Transmission","authors":"Masuyuki Sugiyama,&nbsp;Hideki Hasegawa,&nbsp;Yuichiro Hashimoto","doi":"10.1002/rcm.10136","DOIUrl":"10.1002/rcm.10136","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>Ion transmission efficiency from the atmospheric pressure ion source to the vacuum is critical for the sensitivity of mass spectrometers. In this study, we developed a novel conjugated octupole–quadrupole ion guide—the 8–4 pole ion guide—that achieves high transmission efficiency.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The dependence of signal intensity on parameters, including DC voltages, RF voltage applied to the 8–4 pole ion guide, and pressure within the ion guide, was measured. The transmission efficiency of the 8–4 pole ion guide was estimated from the ion current introduced into the ion guide and passing through the aperture downstream of the guide. The sensitivity of the mass spectrometer equipped with the 8–4 pole ion guide was evaluated using testosterone. Potential calculations and ion trajectory simulations were employed to better understand ion behavior within the 8–4 pole ion guide.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The dependence of signal intensity on pressure within the 8–4 pole ion guide suggests that the device achieves high ion transmission in the pressure range of 100–200 Pa. The ion current introduced into the 8–4 pole ion guide was 1.8 nA, while the ion current passing through the downstream aperture was 1.0 nA, resulting in a transmission efficiency of 56%. The mass spectrometer with the 8–4 pole ion guide achieved a coefficient of variation of 2.9% for 1 pg/mL testosterone. The lower limit of detection for testosterone was 0.12 pg/mL.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>We successfully developed a novel 8–4 pole ion guide. The high transmission efficiency, demonstrated by ion current measurements, and the enhanced sensitivity of the mass spectrometer equipped with this ion guide indicate that it can maintain high transmission efficiency even under a high inlet gas flow of 5 L/min.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 24","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/rcm.10136","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145146701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Untargeted Metabolomics Analysis Combined With Network Pharmacology Reveals Differences in Chemical Profiles and Activities of Different Processed Products of Clematis chinensis Osbeck","authors":"Jianlin Ke,&nbsp;Lei Wang,&nbsp;Lili Huang,&nbsp;Chongchong Li,&nbsp;Jingjing Yang,&nbsp;Chao Zhang","doi":"10.1002/rcm.10143","DOIUrl":"10.1002/rcm.10143","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p><i>Clematis chinensis</i> Osbeck (WLX), an herbal medicine, has been used to treat diseases for thousand years. In clinic, WLX presents four forms, including unprocessed WLX (SZ), WLX mixed with yellow wine (JB), WLX soaked in yellow wine (JJ), and WLX stir-fried with yellow wine (JC). However, few studies have focused on revealing differences in its composition and activity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In this study, metabolomics was used to screen featured metabolites of WLX and its processed products. Subsequently, network pharmacology was applied to reveal the differences in their activity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The results showed that a total of 310 metabolites were identified, and 11 featured metabolites were selected, such as tracheloside, astilbin, and genistein. Network pharmacology analysis suggested that SZ samples mainly exert its specific therapeutic effects by regulating pain pathways, including glutamatergic synapse, spinocerebellar ataxia, and retrograde endocannabinoid signaling. JB, JJ, and JC groups predominantly exert their effects through pathways, including calcium signaling pathway, estrogen signaling pathway, and cAMP signaling pathway, thereby inhibiting the development of inflammatory and pain transmission.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study will provide a theoretical basis for clinical application of WLX and its processed products and offer a reference for their quality control.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 24","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145146434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stable Carbon Isotope Analysis of Perfluorooctanoic Acid (PFOA) by Microflow-High Pressure Liquid Chromatography-Orbitrap Mass Spectrometry","authors":"Paul K. Wojtal,&nbsp;Brett Davidheiser-Kroll,&nbsp;Chad S. Lane,&nbsp;Ralph N. Mead","doi":"10.1002/rcm.10139","DOIUrl":"10.1002/rcm.10139","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Rationale</h3>\u0000 \u0000 <p>As contamination of perfluoroalkyl and polyfluoroalkyl substances (PFAS) in the environment becomes better understood, it is increasingly important to develop forensic tools capable of tracing contamination to emission sources. Isotopic methods have been previously inaccessible for PFAS due to limitations of the instrumentation, but high-pressure liquid chromatography (HPLC) coupled to Orbitrap mass spectrometry (Orbitrap MS) through electrospray ionization (ESI) allows measurement of the isotopic composition of individual PFAS compounds, such as perfluorooctanoic acid (PFOA).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Six separate supplier-procured powders of PFOA were analyzed for their <i>δ</i>¹³C isotopic composition by microflow-HPLC-ESI-Orbitrap MS.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The method presented replicated, within error, the <i>δ</i>¹³C values measured using an elemental analyzer-isotope ratio mass spectrometer (EA-IRMS) for the six different supplier-procured PFOA powders. The offset from the EA-IRMS values for repeated analysis was between 0.2‰ and 1.1‰ and the error was between 0.8‰ and 1.5‰. Our Orbitrap-MS method requires approximately 0.04% of the material required to make an EA-IRMS measurement.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>We developed a method capable of measuring the carbon isotopic composition of PFOA, using HPLC-Orbitrap MS, with high precision and accuracy. This will allow future research to expand analytical capabilities for additional isotopologues of PFOA and other PFAS compounds.</p>\u0000 </section>\u0000 </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 24","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/rcm.10139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}